511 results about "Sporeling" patented technology

The invention discloses a microbial agent and application thereof in preventing and treating rice blast and promoting rice growth, active ingredients of the microbial agent are formed by compounding cold bacillus with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO: 33492 and bacillus velezensis with the preservation number of CGMCC NO: 33493, and the microbial agent aims at solving the synergistic problem of preventing and treating rice blast and promoting rice growth. According to the complex microbial inoculant, through the synergistic interaction between the two strains, the metabolite of the complex microbial inoculant can completely inhibit the spore germination of magnaporthe oryzae; meanwhile, the fungicide can degrade cellulose, obviously promote the growth of plant height, fresh weight and dry weight of rice, and realize the integration of disease prevention and growth promotion. The invention further discloses a preparation method of the fungicide and an application method of the fungicide for preventing and treating rice blast and promoting growth in a root irrigation mode in the three-leaf stage of rice. The microbial agent disclosed by the invention has dual functions of efficiently preventing diseases and stably promoting growth, and an efficient and safe biological solution is provided for green and sustainable development of agriculture.

The invention discloses a hypha mineral composite bionic sand stabilization unit body and a preparation method thereof, and relates to the technical field of sand stabilization, the interior of the unit body is provided with a bionic three-dimensional porous skeleton structure, the unit body comprises a porous core layer, a porous core layer and a porous core layer, the porous core layer takes cleaning sand grains with the particle size of 0.1-2 mm as a sand-based carrier; uniformly mixing and curing with organic nutrient substances, mineral precursors and strain spores; the composite reinforcing layer is formed by interweaving and compounding a mycelium network formed by germination and growth of strain spores, mineral crystals generated by in-situ mineralization induced by metabolic activity of hyphae and a plant fiber reinforcing material which is externally added and embedded into the pores in the pores of the porous core layer; according to the hypha mineral compounded bionic sand stabilization unit body and the preparation method thereof, mold pressing or 3D printing prefabrication is adopted, the structure is controllable, and standardized production and transportation are facilitated; and through a controllable fermentation and gradient dehumidification mineralization culture process, hyphae and minerals are ensured to be uniformly and fully compounded in pores, and the product quality is stable.

The invention discloses a fermentation process of citric acid residues, and relates to the technical field of organic acid fermentation. The fermentation process comprises the following steps: mixing and crushing citric acid residues and wheat bran according to a mass ratio of (4-5): 1 to obtain a mixed material; spraying an ammonia water solution, a magnesium sulfate solution and a zinc sulfate solution into the mixed material, adjusting the pH value to 5.0-5.5, sterilizing, adding a urea solution and a basic nutrient solution, and uniformly mixing to obtain slurry; aspergillus niger and saccharomycopsis fibuligera are mixed according to the spore number ratio of 100: 1, then sterile water is added, the mixture is stirred to be uniform, and bacterial liquid with the spore concentration of 1 * 10 < 7 >-5 * 10 < 7 > / mL is obtained; spraying the bacterial liquid to the surface of the slurry, performing aerobic culture for 40-48 hours under the conditions that the temperature is 30-35 DEG C and the humidity is 80-85%, then cooling to 25-30 DEG C, reducing the humidity to 70-75%, spraying methanol at the same time, and performing sealed culture for 5-6 days; performing drying; and the acid yield and the nutrient substance yield in the finally obtained fermentation product are obviously improved.

The invention discloses a spore microscopic imaging automatic focusing method and system based on a target recognition algorithm, and the method comprises the steps: analyzing the image focusing effect of spore images collected under different object distances in the process of changing the object distance of a microscope, and determining the optimal positive focus object distance based on the object distance corresponding to the spore image with the optimal image focusing effect; the image focusing effect is cooperatively determined based on the number of spores which can be recognized by using a target recognition algorithm in the image and a focusing evaluation value obtained by analyzing the image by using a focusing evaluation function; the image focusing effects of different images are preferentially determined according to the number of the spores which can be recognized; and when the number of the spores is consistent and the image focusing effect difference cannot be determined, further determining the image focusing effects of different images based on the focusing evaluation values of the images. According to the method, the problem of focal plane misjudgment caused by complicated background interference of micro-particle impurities such as pollen and dust in a spore microscopic image is solved.

The invention relates to a direct seeding and seedling raising method for cibotium barometz spores, and belongs to the technical field of plant planting. The invention provides a direct seeding seedling raising method for cibotium barometz spores. The method comprises the following steps: directly seeding the spores to obtain sporophyte bud seedlings; transplanting the sporophyte bud seedlings to obtain sporophyte seedlings; seedling hardening is carried out on the sporophyte seedlings; the sporophyte seedlings are subjected to seedling hardening when the height is 10-15 cm and the number of leaves is 3-4. By means of the direct seeding seedling raising method, the seedling yield is remarkably increased, the seedling yield of the obtained sporophyte seedlings is 85% or above, large-scale industrial popularization and planting can be conducted, and the market supply amount is guaranteed.

The invention provides an agricultural pest diagnosis and early warning method and system based on an intelligent algorithm. The system comprises a spore collection and detection module, a credibility evaluation module, a wind field inversion and source region identification module, a incubation period modeling module, a risk judgment module, a control decision and scheduling module and an output interface module. The spore collection and detection module obtains a spore living body load value in real time through an air sampling and quantitative PCR detection technology; the credible evaluation module realizes pathogen effective trigger state recognition through multi-dimensional threshold judgment; the wind field inversion and source region identification module determines an upstream source region and a propagation path by using a particle trajectory inversion and clustering algorithm; the incubation period modeling module is used for calculating an incubation period and deducing a potential disease time window based on the environmental parameters and an Arrhenius model; quantitative modeling and intelligent early warning of the whole disease and pest propagation process are achieved, the timeliness and accuracy of prevention and control can be improved, and the prevention and control cost is reduced.

The invention belongs to the technical field of plant polysaccharides, and particularly discloses a ganoderma lucidum spore polysaccharide, a preparation method and application of the ganoderma lucidum spore polysaccharide in preparation of medicines for treating tumors. The ganoderma lucidum spore polysaccharide comprises a repetitive structure unit as shown in a formula (I), is a novel uniform-structure polysaccharide separated from ganoderma lucidum spore powder for the first time, has an anti-tumor effect, and is expected to be developed into a new anti-tumor medicine. The preparation method is simple and easy to operate, high in yield, mild in condition and suitable for large-scale production and application.

The invention discloses an intelligent monitoring, prevention and control system for tobacco brown spot, and relates to the technical field of agricultural disease monitoring and intelligent prevention and control. A lower canopy monitoring array, elevation thermal infrared and red edge near infrared imaging and spore sampling (fluorescent quantitative PCR / loop-mediated isothermal amplification) are used as inputs; fog drop deposition D, leaf surface water film W and spore concentration S are predicted through multi-modal alignment and a physical field generator; carrying out long sequence risk assessment by inputting a correlation state space model and superposing a liquid state self-correlation kernel, and carrying out long sequence risk assessment by using concentric wheel patterns and narrow yellow halo (rlt; 0.15) + near-infrared / short-wave infrared black mould layer triple discrimination and checking, and a prescription is formed by combining double-pulse triggering that leaf surface wetting is larger than or equal to 8 h or a spore index reaches a threshold. The prescription comprises night microclimate intervention, variable spraying and leaf thinning control; resistance rotation compliance is realized through prescription uplink pre-check and electronic control unit unlocking; based on row belt / Thiessen polygon scheduling, 36 h coverage is larger than or equal to 95%, the dosage and residues are reduced, and the hit rate and timeliness are improved.

The invention discloses a trichoderma virens preparation capable of preventing diseases and increasing yield as well as a preparation method and application of the trichoderma virens preparation. Belongs to the technical field of biological agents. According to the trichoderma virens strain T259 disclosed by the invention, the trichoderma virens strain T259 is respectively subjected to strain amplification culture, liquid amplification culture and solid fermentation culture, a solid fermentation product of the T259 strain is successfully prepared, the solid fermentation product is mixed with an auxiliary agent, and the trichoderma virens preparation capable of preventing diseases and increasing yield is prepared. The trichoderma virens spore content in the preparation is improved; solid fermentation can effectively improve the colonization ability of trichoderma virens in the field. The production of the trichoderma virens preparation has the characteristics of large sporulation quantity, short period, wide raw material source and low cost; the fertilizer is easy to use and operate, suitable for various crops and remarkable in disease prevention and yield increase effect. And a foundation is laid for quality and efficiency improvement and high-quality and high-efficiency development of modern agriculture.

The invention relates to the technical field of biological prevention and control, in particular to a method for analyzing rice leaf rhythm change after rice is infected by pyricularia grisea and application of the method in accurate prevention and control of rice blast. The analysis method comprises the following steps: (1) spraying a pyricularia grisea spore suspension on rice leaves; (2) carrying out whole-genome transcriptome analysis on the rice leaves, and carrying out clustering heat map; (3) analyzing expression conditions of the biological clock genes and the phytochrome related genes in the circadian rhythm pathway of the rice leaves, and analyzing the biological clock genes and the phytochrome related genes by a MetaCycle method; and (4) analyzing the deposition amount of H2O2 and callose in the rice leaves. Determining the dynamic change rule of the rice immune process caused by magnaporthe oryzae infection according to the method; in combination with the characteristics of small molecules, small-molecule pesticides are reasonably applied at a specific time point aiming at the rice blast, so that efficient and accurate prevention and control of the rice blast are realized.

The invention discloses a Trichoderma harzianum-containing biocontrol fertilizer and a preparation method thereof, and belongs to the technical field of mixed fertilizers, the fertilizer comprises composite matrix particles (containing an organic carrier, an inorganic fertilizer and trace elements), a microsphere coating layer (composed of sodium alginate / chitosan gel microspheres) coated with Trichoderma harzianum on the surface, and a water-soluble fertilizer coating layer (composed of sodium alginate / chitosan gel microspheres) coated with Trichoderma harzianum on the surface, and the outermost layer is a biodegradable protective film (modified starch / PVA / PLA). The preparation method comprises the following steps: granulating a matrix, preparing a bacterial suspension, forming bacteria-containing gel microspheres (the spore recovery rate is greater than or equal to 95%), attaching the microspheres to the matrix, and coating the outer layer. According to the structure, the survival rate of viable bacteria stored at room temperature for 120 days is larger than or equal to 68.3%, the rhizosphere bacterial count is larger than or equal to 4.58 * 10 < 6 > CFU / g soil after the viable bacteria are applied to the soil for 30 days, soil-borne diseases are remarkably inhibited, and the crop yield is increased by 13.5% (cucumber yield is increased by 13.5%).

Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifiations of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.

The present application provides a kind of prevention and control method of green moss in water lily planting pool, specifically belongs to water quality control technical field.The prevention and control method of green moss in water lily planting pool described in the present application includes the following steps: before planting water lily, water injection, water level is controlled at 15~20cm, copper sulfate is applied to kill green moss and its spores, keep water body not to flow during disinfection;After disinfection, apply sodium thiosulfate, and plant water lily;After planting water lily, keep replenishing water, control water level to 40~50cm, when green moss first outbreak, apply mineral fulvic acid potassium and composite bacillus;Subsequent green moss outbreak, apply mineral fulvic acid potassium, or apply mineral fulvic acid potassium and composite bacillus.The prevention and control method described in the present application is safe and efficient, which can quickly and effectively remove green moss without inhibiting the growth of water lily, and also does not affect the harvest of water lily.

The application discloses a LAMP primer group, a kit and a detection method for detecting Pinus armandii rust fungus, and belongs to the technical field of genetic engineering. The application takes the specific nucleotide sequence of the large subunit ribosomal RNA of the Pinus armandii rust fungus as a target gene to design and screen a primer group which is specific and high in sensitivity, and can be used for LAMP detection of the Pinus armandii rust fungus. The LAMP primer group provided by the application has the advantages of high universality, high specificity, high sensitivity and good accuracy, and the detection limit can reach 4.5x10 ‑4 ng / μL, which is 100 times higher than the sensitivity of the LAMP detection of different species in the same genus in the prior art, the detection method can rapidly detect the Pinus armandii rust fungus from spore piles and host plant materials, and is suitable for the detection of Pinus armandii seedling materials in forest fields and customs quarantine ports.

The utility model discloses a spore analyzer, which belongs to the technical field of spore analyzers, and comprises an analyzer body, the upper surface of the analyzer body is fixedly connected with four connecting columns, the upper surfaces of the four connecting columns are fixedly connected with a protective cover, the upper surface of the analyzer body is fixedly connected with a filter cartridge, and the filter cartridge is fixedly connected with a filter screen. The filter cartridge is sleeved with a cleaning device, and a blowing device is fixedly connected into the protective cover. According to the utility model, the cleaning operation of the filter cartridge is realized through the synergistic effect of the handheld groove and the push plate, when an operator puts the hand in the handheld groove to apply thrust, the push plate drives the lantern ring and the scraper to move along the axial direction, and the scraper keeps close contact with the outer surface of the filter cartridge, so that particulate matters accumulated on the surface are effectively stripped in the relative movement process; and the specially designed inclined scraper structure can guide the stripped pollutants to the upper surface of the analyzer body to realize directional removal, so that the permeability of the filter cartridge is maintained.

A process 100 for the decontamination and microbiological stabilisation of liquid egg white stored at room temperature, comprising the operating steps of: preparing the egg white in a liquid state; carrying out a first thermal treatment 110 by carrying out a first and a second sub-step for heating to specific temperature ranges and for specific time intervals in order to eliminate part of the microorganisms present in the egg white and activate any spores present; incubating 130 the egg white at a pre-established temperature and for a specific incubation time interval for the germination of any spores present; carrying out a second thermal treatment 140 on the egg white equivalent to the first to eliminate the spore-derived bacteria; carrying out an isothermal treatment 160 of the egg white to eliminate the bacteria present in the egg white that were not eliminated by the second thermal treatment; carrying out a third thermal treatment 170 similar to the aforesaid first and second thermal treatment in order to eliminate the bacteria present in the egg white that were not eliminated by said first and second thermal treatments.

The application provides a strain of antagonistic cucumber downy mildew Microbacterium luteum C10-2 and application thereof, and belongs to the technical field of biocontrol bacteria. The Microbacterium luteum C10-2 is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20253025. The strain can effectively inhibit cucumber downy mildew. Indoor test shows that the spore germination inhibition rate of the strain on Pseudoperonospora cubensis is 86.54%, the leaf disc prevention efficiency is 84.23%, and the strain is superior to the chemical agent dimethomorph; the fermentation liquor of the strain is sprayed in a field at a concentration of 1x10 8 cfu / mL, and the prevention efficiency reaches 80.50%. The strain can stably colonize in cucumber, inhibit pathogenic bacteria infection, and is environment-friendly, free of drug hazards and not prone to drug resistance. The application provides a new strain resource for green prevention and control of cucumber downy mildew, and has a wide application prospect.

A modified sporulating bacteria may comprise a nucleic acid cassette having a silent promotor that inhibits transcription by the modified sporulating bacteria. The nucleic acid cassette may include a nucleic acid sequence including a gene of interest that encodes a protein of interest. The nucleic acid cassette may include a nucleic acid sequence encoding a loading peptide that incorporates into a coat of a spore. The modified sporulating bacteria may comprise a pre-spore having the nucleic acid sequence having the gene of interest that encodes the protein of interest. A modified spore may be formed comprising a spore body containing the nucleic acid cassette, a spore coat, and a protein of interest derived from the gene of interest inserted into the spore coat made by exogenous cell-free biosynthesis components.

The invention discloses a Ustilaginoidea virens inoculant, a preparation method and application of the Ustilaginoidea virens inoculant in Ustilaginoidea virens inoculation, and belongs to the technical field of agricultural microorganisms. According to the method, two-step shake-flask culture is combined with the steps of filtering, centrifuging and spore germination, so that the ustilaginoidea virens inoculum taking germinated spores (GC) as the core is prepared, and the problems of hypha aging, low spore activity, asynchronous infection and the like in the existing inoculation method are effectively solved. The inoculant has a tender hypha structure and strong infection activity, and the inoculation efficiency and the pathogenesis consistency are remarkably improved. When being used for preparing transcriptome samples, metabolome samples and other omics samples, the strain avoids non-specific immune interference caused by PAMPs released by broken hyphae, ensures synchronous infection process, and is real and reliable in immune response. The invention provides a stable and efficient inoculation system with a clean background for the research on the ustilaginoidea virens-rice interaction mechanism, and has a good application prospect.

The invention provides a streptomyces flavogriseus C-21 strain, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the registration number of the streptomyces flavogriseus C-21 strain is CGMCC No. 36050. The preparation method of the sterile fermentation filtrate of the streptomyces fulvus C-21 comprises the following steps: inoculating a strain C-21 to an oat culture medium plate in a streak manner, culturing for 7 days at 28 DEG C, scraping spores on the surface of the culture medium, adding sterile water to prepare a spore suspension with the concentration of 108 / mL, inoculating the spore suspension into a millet immersion culture solution according to the inoculum size of 2%, and culturing for 7 days at the temperature of 28 DEG C to obtain the sterile fermentation filtrate of the streptomyces fulvus C-21. Performing shaking culture for 7 days in a constant-temperature shaking table at the temperature of 28 DEG C and the speed of 180 r / min, centrifuging for 30 minutes at the speed of 12000 r / min, and filtering supernate by using a 0.22 mu m microporous filter membrane to obtain sterile fermentation filtrate. The streptomyces fulvus C-21 can generate extracellular enzymes such as cellulase, protease and amylase, sterile fermentation filtrate of the streptomyces fulvus C-21 can destroy mycelial morphology and cell membrane integrity of the tomato late blight bacteria, mycelial growth, sporangium germination and zoospore release of the tomato late blight bacteria are remarkably inhibited, and the streptomyces fulvus C-21 has a good control effect on the tomato late blight.

The invention provides a spore solid bacterium inoculation method. Relates to the technical field of edible mushrooms. The method comprises the following steps: S1, culture medium group preparation: selecting trees and setting a spore source culture medium group and a receptor culture medium group in groups; s2, inducing the spore source culture medium group to form a mature sporocarp, inoculating a target edible mushroom strain on the spore source culture medium group, inducing the strain to grow and forming the mature sporocarp; s3, controlling environmental parameters to promote spores to eject and fall on the receptor culture medium group; s4, the sporocarp is obtained through natural culture, the spore source culture medium group accounts for 5%-20% of the total culture medium group in volume, the surface of the receptor culture medium group in the S1 needs to be subjected to scribing treatment, the depth of a notch is 2-5 mm, the condition that the notch makes contact with the xylem is adopted as the criterion, and if the length of the tilia wood is smaller than 30 cm, scribing is not needed. The spore solid bacterium inoculation method provided by the invention has the advantages of reducing the planting cost and improving the economic benefit.

The invention belongs to the technical field of exosome preparation, and discloses a plant exosome with an anti-acne effect and a preparation method and application of the plant exosome with the anti-acne effect. According to the application, an integrated technical route of microbial regulation and induction, complexing-agent-free green extraction and synergistic acne resistance is established: a microbial strain spore suspension is used as a biological inducer, a plant active precursor is released by dynamic countercurrent of ethanol, and PEG / CaCl2 residues are completely eradicated through physical separation in the whole process; finally, the microorganism-induced plant exosome with high yield, high activity and high safety is obtained.

The present invention is in the field of hard surface cleaning compositions. Particularly, it relates to an aqueous hard surface cleaning composition with a good environmental profile that remains stable on storage and provides efficient cleaning and hygiene benefit that sustain for a longer period. There is a need for an improved liquid hard surface cleaning composition with a good environmental profile that provides efficient cleaning and hygiene benefit which sustains for a longer period. It has been found that select Bacillus spore in combination with select copolymers and surfactant provide a storage stable composition. The spore remains viable for germination after storage under various condition and provides efficient cleaning and hygiene efficacy that sustain for a longer period.

The invention relates to a ganoderma lucidum spore oil high-purity deacidification process based on a molecular distillation technology, and discloses a process for removing free fatty acid in ganoderma lucidum spore oil and effectively retaining active ingredients by optimizing parameters of molecular distillation equipment and combining multistage molecular distillation with a nanofiltration membrane pre-concentration process. The purity, the quality and the stability of the ganoderma lucidum spore oil are improved. The method is characterized by comprising the following steps: (1) raw material pretreatment: primarily heating ganoderma lucidum spore oil by adopting a constant-temperature water bath at 30-50 DEG C, removing mechanical impurities by virtue of a vacuum filtering device, then carrying out vacuum drying treatment on the filtered ganoderma lucidum spore oil at 60-70 DEG C for 2-3 hours, controlling the vacuum degree to be 0.01-0.03 MPa, and removing moisture, so as to obtain pretreated ganoderma lucidum spore oil; (2) primary molecular distillation: the ganoderma lucidum spore oil pretreated in the step (1) is subjected to primary molecular distillation, the evaporation surface temperature is 120-140 DEG C, the condensation surface temperature is 40-60 DEG C, the system pressure is maintained within the range of 0.1-0.5 Pa, and light components and heavy components are collected.

The invention discloses an application of trichoderma griseaginii TR2122 in prevention and treatment of beet root rot, a strain of the trichoderma griseaginii TR2122 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.21025, and the strain has strong antagonism and hyperparasitism effects on fusarium oxysporum causing the beet root rot. Meanwhile, the invention further provides a microbial preparation containing the live spores of the strain, the spore content is not lower than 2 * 10 < 8 > CFU / g, and the microbial preparation is preferably wettable powder containing a parent drug, a dispersing agent, a wetting agent and filler. In addition, the invention further discloses a preparation method of the preparation, and the preparation method comprises the steps of fermentation culture, concentration drying and mixing preparation. The strain and the preparation have stable and lasting control effect on beet root rot, are superior to or equivalent to conventional chemical agents, are safe and environment-friendly, and provide an effective solution for beet green control.

The invention belongs to the technical field of agricultural biology, and discloses myrothecium verrucaria with herbicidal activity and application of the myrothecium verrucaria. The strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC M 20251228. Experiments show that the bacterial strain has remarkable pathogenic activity on various weeds such as atrazine, ageratina adenophora, barnyard grass, amaranthus retroflexus and digitaria sanguinalis, the fatality rate of part of weeds under high spore concentration can reach 100%, and the maximum fresh weight inhibition rate can reach 87.47%. More importantly, the strain is safe to the growth of tomato crops and has no obvious adverse effect. The invention also provides application of the strain in preparation of microbial herbicides. The strain and the product thereof provide important strain resources and technical support for developing efficient, safe and environment-friendly novel biological herbicides, and are particularly suitable for green weed control in specific scenes such as tomato fields.

The utility model relates to the technical field of ganoderma lucidum spore oil processing, in particular to storage equipment for decoloring ganoderma lucidum spore oil, which comprises a storage tank, a filter plate is fixedly connected inside the storage tank, a motor is fixedly connected to the bottom of the storage tank, and an output shaft of the motor extends into the storage tank, penetrates through the filter plate and is fixedly connected with a connecting plate. A scraping plate is fixedly connected to one side of the bottom of the connecting plate and makes contact with the top of the filter plate, dirt suction heads are fixedly connected to the interior of one side of the connecting plate at equal intervals, a communicating pipe is fixedly connected between the input ends of the multiple dirt suction heads, and a sediment suction pump is fixedly connected to one side of the storage tank. The ganoderma lucidum spore oil storage device has the advantages that the storage tank effectively isolates oxygen, the temperature control module accurately controls the temperature, the sediment suction pump is connected with the dirt suction heads through the dirt suction pipes, the sediment problem is solved, the quality stability of ganoderma lucidum spore oil in the storage process is comprehensively guaranteed, and the quality guarantee period is prolonged; compared with a traditional device, the operation quality and the use efficiency are greatly improved.

This invention discloses a biocontrol spore cultivation device and its usage method for controlling Japanese pine scale, belonging to the field of pine scale control technology. The device includes an inoculation unit and several cultivation units. The inoculation unit includes a frame with a chain conveyor inside. Multiple positioning support plates are evenly spaced on the chain conveyor. A pre-compression mechanism and an inoculation mechanism are located directly above the chain conveyor on the frame, both spanning the chain conveyor. Several cultivation units are located at the outlet of the inoculation unit. This invention achieves automatic inoculation of biocontrol spores, improving inoculation efficiency and enabling precise control of the inoculation amount, thus improving the uniformity and consistency of biocontrol spore concentration. Next, the inoculated bottles are transferred to multiple cultivation units for static cultivation to improve the viability and yield of biocontrol spores, meeting the needs of large-scale production.

The utility model relates to the technical field of distribution structures, and discloses a noise reduction type spore machine distribution structure which comprises a water inlet pipe and further comprises a pipe sleeve and an installation assembly, an anti-siphon valve for controlling water vapor inside the water inlet pipe to flow is arranged outside the water inlet pipe, connecting pipes are fixedly connected outside the water inlet pipe, and a distribution header pipe is fixedly connected between the connecting pipes. A distribution pipe is fixedly connected into the distribution header pipe, and an installation assembly installed at the outer end is arranged at the right end of the outer portion of the distribution pipe. A part of water-gas mixed liquid continues to flow in the water inlet pipe, the other part of the water-gas mixed liquid enters the connecting pipes, and then the water-gas mixed liquid entering from the water inlet pipe collides with the water-gas mixed liquid in the connecting pipes to form fluid hedging through cooperation of the capillary tube, the friction piece and the damping piece. The sound generated by the impact of the water-gas mixed liquid on the inner wall of the distribution header pipe is reduced, the influence of the noise on the surrounding environment is reduced, and the health condition of workers during working is ensured.

The application provides a kelp breeding performance evaluation method based on sporangium spore dissemination parameters and application, and belongs to the technical field of seaweed cultivation. The method comprises the following steps: S1, taking mature kelp samples, washing, and air-drying treatment to obtain pretreated kelp; S2, taking kelp tissue blocks from the pretreated kelp obtained in step S1, loading the kelp tissue blocks into a mesh bag, immersing the kelp tissue blocks in seawater, promoting the kelp tissue blocks to disseminate, and collecting zoospore liquid; S3, measuring sporangium spore dissemination parameters in step S2; S4, calculating dissemination capacity according to the sporangium spore dissemination parameters obtained in step S3, and evaluating the breeding performance of the kelp according to the dissemination capacity. The above method can quantify the sporangium spore dissemination characteristics of kelp, systematically evaluate the breeding potential of kelp, and improve the breeding efficiency and yield of kelp.