Patents
Literature
Patsnap Eureka AI that helps you search prior art, draft patents, and assess FTO risks, powered by patent and scientific literature data.

875 results about "Sporeling" patented technology

A sporeling is a young plant or fungus produced by a germinated spore, similar to a seedling derived from a germinated seed. They occur in algae, fungi, lichens, bryophytes and seedless vascular plants.

New streptomyces for antagonizing broad-spectrum plant pathogenic fungi and application of new streptomyces

The invention provides a streptomyces, which is named as Streptomyces sp. And is registered and preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 65917. The invention also provides a fermentation broth of the streptomyces, and an ethanol extract and a crude extract of the fermentation broth. The novel streptomyces, the crude extract thereof and the like have broad-spectrum antibacterial activity, have a good antagonistic effect on various pathogenic bacteria such as banana long leaf spot germs and banana colletotrichum gloeosporioides, can enable hyphae of the banana colletotrichum gloeosporioides to shrink, deform and break, inhibit spore germination of the banana colletotrichum gloeosporioides, and have a good anti-bacterial effect on the banana colletotrichum gloeosporioides. According to the present invention, with the application of the bacterial strain, the colletotrichum gloeosporioides spores are shrunk and broken, the growth of the colletotrichum gloeosporioides is inhibited, the peroxidase activity, the polyperoxidase activity, the CAT activity and the like in the banana peel can be improved, and the bacterial strain is the potential biological agent for the prevention and control of the colletotrichum gloeosporioides diseases, and has wide development space and good development and application prospects.
Owner:HAINAN NORMAL UNIV

Seed priming method for improving early-stage disease resistance of pepper seeds

The invention discloses a seed priming method for improving early-stage disease resistance of pepper seeds, which belongs to the field of agricultural planting and comprises the following steps: S1, pre-cleaning: soaking the seeds with sodium hypochlorite and then cleaning the seeds with sterile deionized water; s2, pulse phosphorylation activation treatment: adding seeds and an activation solution into a pressure-resistant polypropylene container connected with a vacuum pipeline, sealing, vacuumizing to-0.08 MPa, maintaining for 5-10 minutes, then recovering to normal pressure, transferring to a constant-temperature oscillator, and carrying out phosphorylation activation treatment; s3, immune training treatment: transferring the seeds subjected to phosphorylation activation treatment to an initiation solution containing UV-inactivated phytophthora capsici spore suspension and H2O2, and carrying out intermittent ventilation treatment; s4, stabilizing treatment: transferring the seeds subjected to immune training treatment into an ascorbic acid solution for treatment, filtering, and drying in the shade to obtain the pepper seeds. According to the invention, the problem of immune response interruption caused by insufficient signal intensity and fast protein turnover when a simulated environment is used for excitation in the prior art is solved.
Owner:VEGETABLE RES INST OF GANSU ACAD OF AGRI SCI

Application of turmeric rhizome extract missible oil in prevention and treatment of corn leaf spot disease and tomato gray mold

The invention relates to application of turmeric rhizome extract emulsifiable concentrate in preventing and treating corn leaf spot and tomato gray mold. Compared with the prior art, the turmeric rhizome extract missible oil disclosed by the invention contains an effective substance aromatic turmerone, and the aromatic turmerone can reduce the spore germination rate and sporulation quantity of pathogenic bacteria, increase the permeability of hypha cell membranes and cause overflow of contents, so that normal growth of hyphae is inhibited, and an antibacterial effect is achieved. Aromatic turmerone acts on plants, so that plant cells generate more polyphenol and flavonoid substances, and the immunity of the plants is improved. Pharmacodynamic test results in fields in two fields show that the 6.5% turmeric rhizome extract emulsifiable concentrate can be used for stably and effectively preventing and treating corn northern leaf blight and tomato gray mold after being diluted by 150-300 times, and also has a yield increasing effect.
Owner:SHANGHAI JIAOTONG UNIV

Method for producing dark septum endophytic fungus cladosporium chlamydospore through liquid fermentation

The invention belongs to the technical field of microorganisms, and particularly relates to a method for producing dark isolated endophytic fungus cladosporium chlamydospore through liquid fermentation. The culture medium and culture conditions suitable for growth of the Cladosporium kwangsiensis HX2 are found out through experiments such as single factor and response surface optimization, the yield of the chlamydospore produced by the Cladosporium kwangsiensis is greatly improved through optimization, the production cost of the chlamydospore is effectively reduced, and the shelf life of the fungicide is prolonged; through measurement and calculation, compared with a traditional PDB culture medium, the yield of chlamydospore is increased by 139.8%, the cost is reduced by 47%, and the shelf life is prolonged by about 8 months. The culture medium is simple in preparation, stable in fermentation process and suitable for large-scale production, the prepared chlamydospore preparation is high in stress resistance and long in shelf life, and a new effective thought is provided for industrialization of bottle cladosporium strains in the later period.
Owner:GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI +1

High-brittleness yellow flammulina velutipes HJ277 and application thereof

The invention belongs to the technical field of edible mushroom cultivation, and particularly relates to a high-brittleness yellow flammulina velutipes HJ277 strain and application thereof. The technical problem to be solved by the invention is to cultivate a flammulina velutipes variety with better quality. According to the technical scheme, the yellow flammulina velutipes HJ277 with the high brittleness is obtained, and the preservation number of the yellow flammulina velutipes HJ277 is CCTCC (China Center for Type Culture Collection) No: M 20242219. The invention also provides a food which comprises the yellow flammulina velutipes, spores of the yellow flammulina velutipes or / and an extract of the yellow flammulina velutipes.
Owner:CHENGDU ACAD OF AGRI & FORESTRY SCI

Lecanicillium lecanii as well as fungicide and application thereof

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to Lecanicillium lecanii as well as a fungicide and application thereof, Lecanicillium lecanii GC-2 is obtained by screening and separating phyllospheric microorganisms, and the strain is proved to have relatively strong inhibitory activity on wheat stripe rust, fusarium graminearum and pseudocereal fusarium; a pot experiment verifies that the strain has a very strong inhibition effect on uredospores of wheat stripe rust, has a relatively strong antagonism effect on pathogenic bacteria of wheat scab and wheat stem rot, has a remarkable prevention and treatment effect on the wheat stripe rust, the wheat stem rot and the wheat scab, has a better prevention effect than a treatment effect, and can be widely applied to prevention and treatment of wheat stripe rust, wheat stem rot and wheat scab. Meanwhile, the strain has no adverse effect on growth and development of wheat and can be applied to prevention and treatment of wheat stripe rust, basal stem rot and gibberellic disease. The invention provides a new strain resource for a biocontrol resource library of plant diseases.
Owner:SHENZHEN RESEARCH INSTITUTE OF NORTHWEST A & F UNIVERSITY

A microbial inoculant for controlling peanut root rot and its preparation method

This invention discloses a microbial inoculant for controlling peanut root rot and its preparation method, belonging to the field of microbial materials technology. This invention screened and obtained a *Trichoderma harzianum* strain with highly efficient inhibitory effects against the pathogen causing peanut root rot. Firstly, the strain itself exhibits antagonistic activity against the peanut pathogen. Secondly, it was prepared as a spore suspension and fermented in solid-state with rice husks and wheat bran. During the solid-state fermentation process, the metabolites produced by *Trichoderma harzianum* promote the growth and development of plant roots, increasing plant biomass and yield. This microbial inoculant effectively controls peanut root rot, exhibiting good inhibitory effects against the pathogen, thus improving peanut quality and disease resistance. Furthermore, it improves soil ecological activity to a certain extent, making it worthy of application and promotion.
Owner:SHANDONG ZHIZHIYUAN ECOLOGICAL ENG CO LTD

Method for inducing formation of nematode-trapping fungus predatory organ

The invention discloses a method for inducing nematode-trapping fungus predatory organ formation, and belongs to the field of applied microorganisms. The method comprises the following specific steps: culturing activated nematode-trapping fungi until spores are generated; washing off the cultured spores, and diluting to obtain a spore suspension with the concentration of 30000-60000 spores / mL; coating the spore suspension into an agar culture medium paved with glass paper, uniformly coating the spore suspension, culturing for 28-36 hours at the temperature of 25-28 DEG C, adding a mixed solution of a predatory organ inducer and water, and continuously culturing for 36-60 hours at the temperature of 25-28 DEG C, so that the nematode-trapping fungi can generate a large number of predatory organs; the predatory organ inducer is one of oleic acid, linoleic acid or pregnenolone. It is found for the first time that a large number of predatory organs can be generated when pregnenolone, oleic acid or linoleic acid is used for inducing nematode-trapping fungi.
Owner:YUNNAN UNIV

Potassium-dissolving microbial inoculum containing yellow microspora fungi, seed coating solution and application of potassium-dissolving microbial inoculum containing yellow microspora fungi and seed coating solution

The invention relates to a potassium-dissolving bacterial agent containing yellow microspora fungi, a seed coating solution and application of the potassium-dissolving bacterial agent and the seed coating solution, and belongs to the technical field of microorganisms. In order to solve the technical blank of the yellow microspora fungi in the aspects of potassium dissolving and kidney bean growth promotion in the prior art, the invention provides the potassium-dissolving microbial inoculum containing the yellow microspora fungi, the yellow microspora fungi are respectively yellow microspora landrace and yellow microspora Xing'an, and the potassium-dissolving microbial inoculum has better potassium-dissolving activity and can be used for preparing the potassium-dissolving microbial inoculum containing the yellow microspora fungi. Symbiosis with plant root systems can be achieved, and plant growth and development are promoted; the kidney bean seed coating liquid containing the potassium bacteria agent can promote accumulation of biomass of kidney bean plants, the setting rate of kidney beans is increased, and the yield of the kidney beans is increased; when the spore concentration is 1 * 10 < 6 > / mL to 1 * 10 < 8 > / mL, the seed coating solution has an obvious and stable growth promoting effect on kidney beans. The potassium-dissolving bacterial agent and the seed coating solution provided by the invention can be applied to soil potassium dissolving and promotion of kidney bean growth.
Owner:HEILONGJIANG UNIV

Microbial agent and application thereof in prevention and treatment of rice blast and promotion of rice growth

The invention discloses a microbial agent and application thereof in preventing and treating rice blast and promoting rice growth, active ingredients of the microbial agent are formed by compounding cold bacillus with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO: 33492 and bacillus velezensis with the preservation number of CGMCC NO: 33493, and the microbial agent aims at solving the synergistic problem of preventing and treating rice blast and promoting rice growth. According to the complex microbial inoculant, through the synergistic interaction between the two strains, the metabolite of the complex microbial inoculant can completely inhibit the spore germination of magnaporthe oryzae; meanwhile, the fungicide can degrade cellulose, obviously promote the growth of plant height, fresh weight and dry weight of rice, and realize the integration of disease prevention and growth promotion. The invention further discloses a preparation method of the fungicide and an application method of the fungicide for preventing and treating rice blast and promoting growth in a root irrigation mode in the three-leaf stage of rice. The microbial agent disclosed by the invention has dual functions of efficiently preventing diseases and stably promoting growth, and an efficient and safe biological solution is provided for green and sustainable development of agriculture.
Owner:SHENYANG AGRI UNIV

Method for rapidly propagating spores of cyatheridae rare and valuable fern type pteridium aquilinum

PendingCN120266723AGrowth substratesCulture mediaSporelingSporophyte
The invention discloses a rapid spore propagation method of a cyatheridae rare fern type pteridium aquilinum var. Spinulosa var. Spinulosa. Comprising the following steps: (1) collecting and storing spores; (2) selecting and preparing a spore culture medium; (3) spore treatment and sowing; (4) culturing spores; (5) observing and maintaining gametophytes; (6) transplanting sporophyte young plants; (7) seedling management and protection; (8) transplanting seedlings. When seedlings in the seedling raising box grow to about 5-8 cm high, the seedling raising box is moved into a non-imitation cloth seedling raising bag in an outdoor greenhouse nursery. Compared with tissue culture, the method has the advantages of high success rate, simplicity and high efficiency. A manual climatic box is not needed in a spore-gametophyte cultivation stage, but on a light-cared cultivation frame, so that the operation is simple and easy, and the limitation of space is avoided.
Owner:SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI

Method for improving embryogenesis rate of free microspores of non-heading Chinese cabbage by using 5-AzaC

The invention relates to the technical field of plant tissue culture, and provides a method for improving the embryo emergence rate of free microspores of non-heading Chinese cabbage by using 5-AzaC, and in the microspore culture stage, 5-AzaC with the concentration of 0.005-0.020 mg is added into a microspore culture medium. The invention designs a method for increasing the embryogenesis rate of free microspores of the non-heading Chinese cabbage by adding 5-AzaC into a microspore culture medium of the non-heading Chinese cabbage. According to the method, the embryogenesis rate of the free microspores of the non-heading Chinese cabbage can be remarkably improved, a non-heading Chinese cabbage microspore culture system is more easily established, the haploid breeding efficiency is improved, and the breeding process is accelerated.
Owner:ANHUI AGRICULTURAL UNIVERSITY +1

Spiropolyspora pink strain for preventing and treating root knot nematode disease as well as fungicide and application of spiropolyspora pink strain

The invention provides a spiropolyspora roseus strain for preventing and treating root knot nematode disease, and the preservation number of the spiropolyspora roseus strain is CGMCC No.41822. The invention also provides a microbial inoculum containing the spiropolyspora pink strain, and the microbial inoculum is prepared by the following steps: inoculating a culture medium with the spiropolyspora pink HLF-14-11, and culturing until spores are produced; spores are eluted to prepare a spore suspension, or a bacterial block is cut to serve as a liquid fermentation inoculant; inoculating the inoculant into a liquid culture medium, and carrying out shake culture; and taking the fermentation liquor to prepare liquid, powder and granules. The spiropolyspora pink strain provided by the invention is separated from root-knot nematode oocysts of diseased tomatoes, can parasitize eggs of root-knot nematodes and inhibit incubation of the eggs, fermentation filtrate of the spiropolyspora pink strain has a relatively strong poisoning effect on second-stage larvae of the root-knot nematodes, and the fatality rate of the second-stage larvae reaches 89.1%. Meanwhile, the strain can produce auxin substances such as indoleacetic acid and the like, and plant growth and root development are promoted.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI

Artificial cultivation method of xylaria fungus sclerotium

The invention discloses an artificial cultivation method of xylaria fungus sclerotium, and relates to the field of sclerotium cultivation. The method comprises the following steps: collecting xylaria producing sclerotia, obtaining spores by adopting a spore separation method or obtaining core tissues by adopting a tissue separation method, inoculating the spores or the core tissues on an enriched PDA culture medium to be cultured into bacterial colonies, selecting hyphae with vigorous growth vigor at the edges of the bacterial colonies, and performing purification culture until a mother strain only containing a single strain is obtained; performing gene sequencing to identify the strain; propagating the mother strain to obtain an original strain, inoculating the original strain into a sterilized solid culture medium, culturing in a dark place to obtain a cultivated strain, placing the cultivated strain on soil, reserving a space for sclerotium development around the cultivated strain, culturing in the dark place for 33-90 days, and harvesting the sclerotium. The xylaria sclerotium with single germplasm can be cultivated on a large scale, and the problems of wild resource shortage and germplasm mixing are effectively solved.
Owner:RENMIN HOSPITAL OF WUHAN UNIVERSITY (HUBEI GENERAL HOSPITAL)

Fusion proteins, recombinant bacteria, and methods for using recombinant bacteria

ActiveUS12391729B2Waste water treatment from quariesBacteriaFusion Protein ExpressionSporeling
Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifications of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.
Owner:SPOGEN BIOTECH INC

Attenuation of phytofungal pathogenicity by autophagy modulators

PCT designated stage expiredWO2025151885A1BiocideDead plant preservationBiotechnologySporeling
The present invention is in the field of agricultural pathogen control. Methods and compositions are described for controlling plant diseases caused by pathogens, including fungi and oomycete phytopathogens. Chemicals were screened for activity as autophagy inhibitors. The screening method used a bioluminescence resonance energy transfer (BRET)-based high-throughput screening strategy to identify compounds that inhibit fungal ATG4 cysteine protease-mediated cleavage of ATG8 for autophagosome formation. Ebselen and ebselen analogs were identified as inhibitors of fungal pathogens ATG4-mediated ATG8 processing. Identified chemicals inhibit spore germination, hyphal development, and appressorium formation. The autophagy inhibitors significantly reduced the virulence of pathogens in their corresponding plant hosts. The invention also provides for compositions comprising the same, methods of making the same, and methods of controlling plant disease.
Owner:UNIVERSITY OF WYOMING

E3 ubiquitin ligase gene ulA and application thereof

The invention discloses an E3 ubiquitin ligase gene ulA, the nucleotide sequence of which is shown as SEQ ID NO: 1. The gene is knocked out from fusarium oxysporum by using a homologous recombination method to obtain a mutant strain ulA; compared with a wild strain, the mutant hulA has the advantages that the spore yield is reduced, the spore germination is delayed, the response capability to various abiotic stresses is reduced, the pathogenicity to panax notoginseng plants is reduced, and the sensitivity to six bactericides is increased, and experimental results show that the gene plays an important role in pathogenicity, drug resistance and the like of fusarium oxysporum. The gene can be used as a target gene for developing an anti-fusarium oxysporum preparation.
Owner:KUNMING UNIV OF SCI & TECH

Hypha network reinforced ecological slope protection structure and construction method

The invention discloses a hypha network reinforced ecological slope protection structure and a construction method.The hypha network reinforced ecological slope protection structure comprises a plurality of ecological soil nails arranged on a side slope, and each ecological soil nail comprises a soil nail head located outside the side slope; a hollow shell extending into the slope is arranged at one end of the soil nail head, a grouting hole communicated with the hollow shell is formed in the soil nail head, and a plurality of leakage holes are formed in the surface of the hollow shell; a plurality of ecological panels are arranged on the inclined face of the side slope, and the ecological soil nails are filled with grouting liquid with hypha spores. An assembly type structure frame is formed by inserting and matching the ecological panels and the ecological soil nails, grouting liquid with spore bags is injected, after the spore bags are activated, mycelium is combined in a winding mode in the growth process, and a high-ductility biological fiber network structure is constructed between soil particles and components. And the structural integrity and the shear resistance and disturbance resistance of the side slope are improved.
Owner:WUHAN UNIV

Fluorescence image recognition system for detecting fungi based on deep learning algorithm

The invention provides a fluorescence image recognition system for detecting fungi based on a deep learning algorithm. The fluorescence image recognition system comprises an image acquisition module, a single spore and hypha recognition module and a hypha group recognition module, the image acquisition module is used for acquiring a target fluorescence image; and the single spore and hypha identification module and the hypha mass identification module are used for inputting the target fluorescence image into a pre-established deep learning algorithm model, identifying the single spore or hypha and the hypha mass through the pre-established deep learning algorithm model, and outputting a fungus negative or positive result according to an identification result. Compared with the prior art, after the target fluorescence image is obtained by the image acquisition module, the target fluorescence image is input into the pre-established deep learning algorithm model for automatic analysis, and analysis of spores / hyphae and hypha masses which are greatly different in form is divided into two modules for processing; and spores, hyphae and hypha masses can be identified and distinguished more accurately.
Owner:AFFILIATED HUSN HOSPITAL OF FUDAN UNIV +1

Application of demethylzeylasteral in preparation of product for preventing and treating phytophthora

The invention belongs to the technical field of modern agriculture, and particularly relates to application of demethylzeylasteral (DZL) to preparation of a product for preventing and treating phytophthora. The invention determines the effect of pentacyclic triterpenoid demethylzeylasteral on activity inhibition of phytophthora capsici and other crop phytophthora, and demethylzeylasteral can inhibit phytophthora, sporangium release, zoospore germination and pathogenicity, which indicates that demethylzeylasteral (DZL) has the effect of resisting phytophthora capsici. Therefore, the compound provided by the invention can be used for preparing a small-molecule inhibitor for growth, development and pathopoiesis of phytophthora of crops, is expected to become a potential drug for resisting phytophthora of crops, and has good development and application values.
Owner:HAINAN UNIVERSITY SANYA NANFAN RESEARCH INSTITUTE +1

Metarhizium ledebusii GZFW2211 and application thereof

The invention provides metarhizium ledebusii GZFW2211 and an application of the metarhizium ledebusii GZFW2211. The green muscardine fungus GZFW2211 is preserved in Guangdong Microbial Culture Collection Center on February 13, 2025, and the preservation number is GDMCC No: 65784. The spodoptera frugiperda spore suspension is a strain separated from spodoptera frugiperda larvae, the strain is high in growth speed, short in conidium production time and large in spore production quantity, and the prepared spore emulsion suspension is high and stable in pathogenicity to important pests such as spodoptera frugiperda, prodenia litura and agrotis ypsilon. The insecticidal composition can be widely used for preventing and treating pests such as spodoptera litura, prodenia litura and agrotis ypsilon.
Owner:INST OF ZOOLOGY GUANGDONG ACAD OF SCI

Mycelium mineral compounded bionic sand stabilization unit body and preparation method thereof

The invention discloses a hypha mineral composite bionic sand stabilization unit body and a preparation method thereof, and relates to the technical field of sand stabilization, the interior of the unit body is provided with a bionic three-dimensional porous skeleton structure, the unit body comprises a porous core layer, a porous core layer and a porous core layer, the porous core layer takes cleaning sand grains with the particle size of 0.1-2 mm as a sand-based carrier; uniformly mixing and curing with organic nutrient substances, mineral precursors and strain spores; the composite reinforcing layer is formed by interweaving and compounding a mycelium network formed by germination and growth of strain spores, mineral crystals generated by in-situ mineralization induced by metabolic activity of hyphae and a plant fiber reinforcing material which is externally added and embedded into the pores in the pores of the porous core layer; according to the hypha mineral compounded bionic sand stabilization unit body and the preparation method thereof, mold pressing or 3D printing prefabrication is adopted, the structure is controllable, and standardized production and transportation are facilitated; and through a controllable fermentation and gradient dehumidification mineralization culture process, hyphae and minerals are ensured to be uniformly and fully compounded in pores, and the product quality is stable.
Owner:INNER MONGOLIA AGRICULTURAL UNIVERSITY

Method for large-scale seedling culture of rhizoma drynariae

The invention relates to a large-scale seedling raising method for rhizoma drynariae. The method comprises the steps of rhizoma drynariae greenhouse transplanting, seedling raising pretreatment, rhizoma drynariae spore collection, spore germination, rhizome formation promotion, conventional seedling management and the like, and a large number of seedlings can be obtained. According to the method, fortune's drynaria rhizome spores are promoted to germinate on a camphor tree sawdust substrate through weak illumination and a nutrient solution, and the problems that fortune's drynaria rhizome spores do not germinate due to water shortage and cannot form seedlings due to nutrition deficiency are effectively avoided The growth environment of the rhizoma drynariae cultivated in a greenhouse is fully utilized, and the seedling culture space is saved and the seedling culture cost is greatly reduced by modifying the seedling culture substrate and directly breeding spores; by means of an existing hanging spraying facility, water and nutrient supply for seedling raising is guaranteed, the labor cost is reduced, the spore germination rate and the seedling survival rate are greatly increased, and after small rhizomes are directly adopted for transplantation in subsequent management, the spore collection and sowing difficulty is reduced, and the seedling cultivation time is effectively shortened.
Owner:ZHANGJIAJIE XINNONG AGRI TECH CO LTD

Aptamers against clostridium difficile

Compositions comprising aptamers capable of specifically binding to a surface protein of Clostridium difficile spore are provided. A method for detecting, enriching, separating, and / or isolating Clostridium difficile spores is provided.
Owner:LIV PROCESS INC

Bacillus atrophaeus for preventing and treating wheat gibberellic disease caused by gibberellic maize and application of bacillus atrophaeus

The invention relates to the technical field of biological control of plant diseases, in particular to application of a bacillus atrophaeus strain and a preparation thereof to control wheat gibberellic disease caused by gibberellic maize. The preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.29799, and the strain is classified and named as the bacillus atrophaeus strain HHQGTS13-1 (Bacillus atrophaeus HHQGTS13-1). The bacillus atrophaeus strain HHQGTS13-1 is wide in application range to natural environment conditions such as temperature and pH, and has the capability of antagonizing fusarium maydis when the temperature is 10-45 DEG C and the pH value is 4-10. The bacillus atrophaeus strain HHQGTS13-1 can be used for obviously inhibiting the growth of fusarium maydis hyphae and enabling the hyphae to be deformed; the antagonistic bacillus HHQGTS13-1 strain has an obvious inhibition effect on spore germination of fusarium maydis, the spore germination inhibition rate is 97.10%, the prevention and treatment effect of the antagonistic bacillus HHQGTS13-1 strain fermentation liquor after wheat is inoculated with fusarium maydis pathogenic bacteria is 63.43%, and the antagonistic bacillus HHQGTS13-1 strain has good potential of preventing and treating plant diseases caused by fusarium maydis.
Owner:NANNING HARWORLD BIOLOGICAL TECH CORP +2

Biological indicator reader systems for determining efficacy of sterilization

A biological indicator includes: a BI housing; a germinant container inside the BI housing and housing a germinant composition; a germinant releaser configured to release the germinant composition from the germinant container; a germinant releaser support supporting the germinant releaser and configured to bring the germinant releaser into contact with the germinant container upon application of a force to the germinant releaser support or the germinant container; a first spore carrier inside the BI housing, the first spore carrier having a plurality of spores deposited at a first surface thereof; and an imaging window at a first surface of the BI housing. A BI reader is configured to detect and quantify the presence of live spores in the BI, and includes an excitation source, a camera for capturing images of the spores over time, and a processor for analyzing the images to determine the presence of live spores.
Owner:STERITEC PRODUCTS MANUFACTURING CO INC

Fermentation process of citric acid residues

PendingCN121465153AAnimal feeding stuffSporelingSpore Count
The invention discloses a fermentation process of citric acid residues, and relates to the technical field of organic acid fermentation. The fermentation process comprises the following steps: mixing and crushing citric acid residues and wheat bran according to a mass ratio of (4-5): 1 to obtain a mixed material; spraying an ammonia water solution, a magnesium sulfate solution and a zinc sulfate solution into the mixed material, adjusting the pH value to 5.0-5.5, sterilizing, adding a urea solution and a basic nutrient solution, and uniformly mixing to obtain slurry; aspergillus niger and saccharomycopsis fibuligera are mixed according to the spore number ratio of 100: 1, then sterile water is added, the mixture is stirred to be uniform, and bacterial liquid with the spore concentration of 1 * 10 < 7 >-5 * 10 < 7 > / mL is obtained; spraying the bacterial liquid to the surface of the slurry, performing aerobic culture for 40-48 hours under the conditions that the temperature is 30-35 DEG C and the humidity is 80-85%, then cooling to 25-30 DEG C, reducing the humidity to 70-75%, spraying methanol at the same time, and performing sealed culture for 5-6 days; performing drying; and the acid yield and the nutrient substance yield in the finally obtained fermentation product are obviously improved.
Owner:HEFEI FISUGARPEPTIDE BIOTECH CO LTD

Wheat scab spore germination form detection method based on microscopic image

The invention relates to a wheat gibberellic disease spore germination form detection method based on microscopic images. Compared with the prior art, the wheat gibberellic disease spore germination form detection method solves the defect that germination form spore microscopic images are difficult to detect. The method comprises the following steps: collecting and preprocessing a wheat scab spore microscopic image; construction of a germination form target key point detection model; training a germination form target key point detection model; acquiring and preprocessing a medicament treatment spore image; and outputting a spore detection result. Through the germination convolution cascade module and the germination recursion gating module, spore sub-pixel level details and a germination tube global contour are accurately captured in a microscopic image, the problem of missing detection and false detection of tiny targets is solved, and microscopic image accurate detection of germinated spores is realized.
Owner:NORTHWEST A & F UNIV

Macrosporium globosum capable of efficiently producing spores and promoting plant growth and application of macrosporium globosum

The invention discloses a strain of globosoma macrosporium capable of efficiently producing spores and promoting plant growth and application of the globosoma macrosporium. The preservation number of the globular cystospora margarita LBN1103 is GDMCC (China General Microbiological Culture Collection Center) No: 66650, and the preservation number of the globular cystospora margarita LBN1103 is GDMCC No: 66650. The globular cystomycosis LBN1103 has extremely high spore production capacity, and the spore production proportion is about 1: 353.4 and is 5.20-18.03 times that of globular cystomycosis strains reported in other literatures. On the premise that the inoculation amount is very low, the biomass of paspalum natatum is remarkably increased by inoculating the globular cystosporium macrosporum LBN1103 into the sterilized soil, and the biomass of tomatoes is also remarkably increased by inoculating the globular cystosporium macrosporum LBN1103 into the unsterilized soil. The globosporium macrosporum LBN1103 disclosed by the invention is high in spore production efficiency and strong in ecological niche competitive advantage, obviously promotes plant growth, and has a wide application prospect in the aspects of biological reagents, biological agriculture and the like.
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY