10 results about "Microspore" patented technology

The invention relates to a haploid induction method based on a brassica napus phospholipase gene BnaPLA2-a and application of the haploid induction method, the BnaPLA2-a gene in brassica napus is knocked out through a gene editing technology, a mutant material capable of inducing haploid generation is obtained, and the application blank of a phospholipase pathway in dicotyledon haploid induction is filled. Compared with a traditional microspore culture technology, the invention provides a brand-new haploid induction path which is derived from the rape and is used for the rape. The invention provides a brand new technical tool and germplasm resources for genetic breeding of brassica napus. Meanwhile, the invention discloses a gene, a mutant, a creation method and application in breeding.

The invention relates to the technical field of bioengineering, in particular to a method for extracting exosomes from microspore ganoderma lucidum immune protein.The method comprises the steps that microspore ganoderma lucidum immune protein extraction comprises pretreatment, extraction, centrifugation, salting-out, dialysis and chromatographic purification; exosome is extracted from cell culture supernate by using the immune protein through an immunoaffinity capture method, and the method comprises the steps of incubation combination, centrifugal separation and elution purification. The microspore ganoderma lucidum immune protein is specifically combined with the exosome for the first time, the extraction method is efficient, mild and low in cost, and the extracted exosome is high in purity and good in integrity, has double activities of the exosome and the ganoderma lucidum immune protein, and has wide application prospects in the fields of disease diagnosis, drug delivery, immune regulation and the like.

Plant cell fate and development is altered by treating cells with cellular reprogramming factors. Embryogenesis inducing embryogenesis factor genes and / or morphogenic developmental genes are used as cellular reprogramming factors, specifically comprising polypeptides or polynucleotides encoding gene products for generating doubled haploids or haploid plants from gametes. Maize microspores treated by contacting the isolated cells with an exogenous purified, recombinant embryogenesis inducing embryogenesis factor gene products and / or morphogenic developmental gene polypeptide results in embryogenesis. The gametes of a maize plant develop into embryoids when transformed with a genetic construct including regulatory elements and structural genes capable of acting in a cascading fashion to alter cellular fate of plant cells. Embryogenesis factor proteins and / or developmental morphogenic proteins expressed from a genetic construct are used for ex situ treatment methods and for in planta cellular reprogramming.

This disclosure provides automated, high-throughput methods and systems for generating microspore-derived doubled haploid structures, plantlets, and crop plants, and progeny thereof. Methods of integrating computer vision and artificial intelligence into a variety of steps including microspore selection, culture, sorting, and genotyping are disclosed. Multiplexed genome-editing of microspores through cargo delivery, non-destructive sampling, and genotyping are also described.

The invention provides an anther liquid culture method of hybrid highland barley, and belongs to the technical field of plant tissue culture. The method comprises the following steps: (1) refrigerating young ears of hybrid highland barley, disinfecting, and collecting anther; (2) inoculating the collected anther into a barley pre-culture solution for culture to obtain a pre-cultured anther; (3) inoculating the pre-cultured anther into a barley induction culture medium for culturing to obtain a callus; (4) inoculating the callus into a differential culture medium for culturing to obtain a green regenerated plant; and (5) inoculating the green regenerated plant into a strong seedling culture medium for culturing to obtain the highland barley seedling. According to the method, single ears are cultured in dishes, the damage and cross contamination probability of a mechanical dissociation mode to microspores is reduced, and the method is a small-scale culture mode suitable for being limited by material drawing. The method solves the problems that the number of materials of hybrid highland barley is limited and the activity of anther free microspores is relatively weak, and has a wide application prospect.

The application discloses a kind of multiple qPCR detection primer combinations of superficial mycosis pathogen, it includes the specific primer and probe of detecting Trichophyton rubrum, Trichophyton mentagrophytes, Epidermophyton floccosum, Microsporum canis, Microsporum audouinii, Microsporum gypseum, Candida albicans, Malassezia furfur;The application detects pathogen target gene using real-time fluorescent quantitative PCR technology;The method has the advantages of short time consumption, high specificity, low cost, and can detect multiple pathogens at a time, provides a convenient method for the detection of superficial mycosis pathogen, and has important significance for the early molecular diagnosis of superficial fungal infection patients.

The present disclosure provides novel methods and compositions for the maturation and regeneration of plantlets from microspore-derived embryos. The compositions provided herein include a liquid maturation composition comprising a first plant hormone and about 430 mM to about 880 mM of a monosaccharide solute, a disaccharide solute, or a polysaccharide solute. The methods provided herein include the steps of contacting a microspore-derived embryo with a liquid maturation medium for a time period sufficient to produce a matured microspore-derived embryo, transferring the matured microspore-derived embryo to a substrate, and regenerating the plantlet from the matured microspore-derived embryo.

The invention discloses a rape high oleic acid Pol restorer line breeding method and application thereof, and relates to the technical field of rape breeding, the breeding method comprises the following steps: hybridizing a high oleic acid content line with a common oleic acid content Pol restorer line fully fertile plant, and combining a rape microspore technology, molecular marker identification and gas chromatography to breed a high oleic acid Pol restorer line material; the molecular marker comprises an oleic acid KASP marker, and primer sequences of the oleic acid KASP marker are shown in SEQ ID NO: 1-SEQ ID NO: 3. According to the molecular marker and the breeding method thereof, single plants can be effectively selected according to genotypes at any period in the breeding process, the selection efficiency is improved, the breeding period is greatly shortened through the rape microspore technology, the breeding efficiency is improved, and the efficient breeding method is provided for breeding of the high-oleic-acid rape Pol restoring line.

The invention provides a breeding method for obtaining haplobionts through apple anther culture, and belongs to the technical field of plant breeding. Comprising the following steps: selecting flower buds with microspores in the late uninucleate stage, carrying out low-temperature pretreatment and heat shock treatment, disinfecting and sterilizing, stripping anther, sequentially selecting corresponding culture mediums, and carrying out induction culture, differentiation culture, subculture multiplication and rooting culture to obtain apple test-tube plantlets, acclimatizing and transplanting to obtain apple haplobionts. According to the method, low-temperature pretreatment cooperates with short-time heat shock treatment, the culture medium in each culture stage is optimized, the efficient, stable and repeatable method for obtaining the haplobiont through apple anther culture is established, the induction rate and the differentiation rate can be increased, and the malformation rate can be reduced.

The invention provides a method for establishing an ATAC-seq library by using quinoa microspores, and belongs to the technical field of bioengineering. By improving a microspore sample pretreatment process and adjusting parameters of a transposase reaction system, key technical bottlenecks in the prior art are solved, efficient construction of the quinoa microspore ATAC-seq library is realized, and reliable technical support is provided for analyzing a chromatin regulatory network developed by quinoa male gametophytes and excavating and regulating key genes.