115 results about "Oryza" patented technology

The invention discloses application of a rice OsbHLH083 gene in regulation and control of seed germination capability, and relates to the technical field of plant genetic engineering. The CDS sequence of the rice OsbHLH083 gene is as shown in SEQ ID NO. 2, and the amino acid sequence of the coded protein is as shown in SEQ ID NO. 3. The molecular biology function of the rice OsbHLH083 gene for regulating rice germination is studied, after the rice OsbHLH083 gene is edited through CRISPR / Cas9, compared with a wild type, the rice OsbHLH083 gene mutant rice seeds germinate slower, the rice OsbHLH083 gene and a protein coded by the rice OsbHLH083 gene can be used for regulating the phenotype of seed germination, and the rice OsbHLH083 gene mutant rice seeds germinate more slowly. And a gene resource is provided for the genetic improvement of rapid germination and seedling formation characters of the direct seeding rice seeds in the field.

The invention relates to the technical field of plant genetic engineering, and discloses a haplotype identification marker of a rice flood-resistant gene ARF9 and application of the haplotype identification marker. The molecular marker is derived from an exon of an ARF9 gene, is located at the 22009082bp and 22010019bp of a No.4 chromosome of rice, and has a basic group of C. The SNP molecular marker is related to the waterflooding resistance of a male parent oryza longistaminate of perennial rice, and the waterflooding resistance of the male parent oryza longistaminate with a locus genotype of CC is obviously higher than that of rice with a locus genotype of non-AA type. Therefore, the invention provides the loop-mediated isothermal amplification primer group for rapidly identifying the ARF9 gene haploid molecular marker, and the loop-mediated isothermal amplification primer group is used for identifying the ARF9 gene haploid molecular marker so as to rapidly screen out waterflooding-resistant rice. The invention provides a group of primers, the flood-resistant rice can be quickly identified by adopting the primers to perform loop isothermal reaction, the detection cost is low, the detection speed is high, the flood-resistant germplasm can be quickly screened in the rice seedling stage by adopting the method provided by the invention, a time-consuming flooding test does not need to be performed in the later stage, and the breeding process is greatly accelerated.

The invention discloses application of rice protein MBS and a coding gene thereof in plant light protection. The invention provides any one of the following applications of the protein OsMBS. C1, regulating and controlling the plant yield; c2, regulating and controlling light protection of the plants; c3, regulating and controlling the strong light adaptability of the plants; c4, regulating and controlling the photosynthetic activity of the plants; and C5, cultivating a strong light resistant plant. Transgenosis experiments and field yield measurement verify that the homologous protein OsMBS has enhanced expression in rice, the chloroplast is wrapped in a sharp increase mode under strong light, a shading layer for directly protecting the chloroplast is formed, light protection of the rice fragrance of the rice can be enhanced, and remarkable and efficient yield increase in the field is achieved.

The invention provides application of Ustilaginoidea virens secretory protein UvWMT and a coding gene thereof in prevention and control of plant diseases. The gene sequence of the Ustilaginoidea virens secretory protein UvWMT is researched, found and cloned for the first time, and the new application of the Ustilaginoidea virens secretory protein UvWMT in disease-resistant breeding of crops such as rice is developed. Research finds that the protein is a key pathogenic factor in the process that rice is infected by ustilaginoidea virens, heterologous expression experiments further find that heterologous expression of UvWMT in rice can significantly improve the resistance of crops to various pathogenic bacteria such as ustilaginoidea virens, magnaporthe oryzae and xanthomonas oryzae, and has no significant difference in key agronomic traits compared with a wild type. An important theoretical basis is provided for rice disease-resistant breeding, crops can be safely and effectively endowed with broad-spectrum disease resistance by heterologous expression of the gene, and the gene has great application prospects and economic values in the aspects of crop breeding disease resistance improvement, rice disease prevention and control and crop yield increase and pesticide reduction.

The invention provides an application of a Ustilaginoidea virens UvWSC1 gene in improvement of plant disease resistance. The invention develops a new application of the Ustilaginoidea virens UvWSC1 gene in improving the broad-spectrum disease resistance of crops such as rice for the first time, and the UvWSC1 gene is subjected to heterologous high expression in the rice, so that the broad-spectrum resistance of the rice to various pathogenic bacteria such as Ustilaginoidea virens, Magnaporthe oryzae and Xanthomonas oryzae can be obviously enhanced, and the agronomic traits of the rice are not influenced; an important theoretical basis is provided for rice disease-resistant breeding. By heterologous expression of the UvWSC1 gene, broad-spectrum disease resistance can be safely and effectively endowed to crops, a broad-spectrum disease-resistant rice variety is obtained for production, and the broad-spectrum disease-resistant rice gene has great application prospects and economic values in the aspects of crop breeding disease resistance improvement, rice disease prevention and control and crop yield increase and pesticide reduction.

The invention discloses a method for in-vitro preservation of common wild rice and Nwa wild rice, and belongs to the technical field of plant tissue culture. The method comprises the following steps: selecting a stem node with an axillary bud as an explant; soaking in 75% alcohol for 10-15 seconds, soaking in 10% 84 disinfectant for 15 minutes, performing vacuum treatment in 0.1% mercuric chloride solution under-0.1 Mpa for 5 minutes, and performing water bath at 40 DEG C for 20 minutes for combined sterilization; inserting the sterilized explant into a bud differentiation culture medium containing 2mg / L KT and 0.1 mg / L NAA, and culturing for 3-5 days under the conditions that the temperature is 23 DEG C and illumination is performed for 16 hours per day; cutting off buds growing to 3-5cm, and transferring the buds into a rooting culture medium; (5) carrying out test-tube plantlet propagation through stem node shearing or tillering cutting; the tissue culture seedlings with well developed root systems are hardened and then transplanted to a mixture of vermiculite and nutrient soil (v / v = 1 / 1). According to the method, the pollution rate is as low as 16.35%, the differentiation rate of the explant reaches 47.36%, the process is simple, convenient and efficient, the production period is short, the seed quality resources of common wild rice and Nilval wild rice can be effectively preserved, and the method has important significance on protection of precious wild rice resources.

The invention relates to the technical field of agriculture, and particularly provides a CAPS molecular marker of a rice salt-tolerant ST4 promoter region and application of the CAPS molecular marker. The method comprises the following steps: selecting leaves of rice to extract genome DNA (Deoxyribonucleic Acid) of the tissues, and then respectively carrying out PCR (Polymerase Chain Reaction) amplification by taking the DNA of the tissues as a template and taking corresponding sequences of ST4-PRO-F1 and ST4-PRO-R1 as specific primers to obtain amplified fragments; carrying out enzyme digestion on the amplified fragment by using Eco81I restriction enzyme, and carrying out electrophoresis after enzyme digestion; after the ST4 promoter fragment is subjected to electrophoresis, a single plant with only one 2162bp main band is a salt-tolerant single plant, and a single plant with 1366bp and 784bp bands appearing in electrophoresis is a salt-sensitive single plant. According to the invention, the rice variety improved by utilizing the wild rice ST4 gene can be rapidly bred, the current situations of much field work and large workload caused by carrying out a large amount of salt tolerance identification by utilizing a population material can be improved, and the method has important theoretical and practical significance for breeding a new variety of rice with salt tolerance character.

The present application relates to the application of allantoin in the prevention and treatment of fungal diseases of grain crops. The present application finds that allantoin can inhibit the growth of pathogenic fungi of grain crops, especially inhibit the infection of pathogenic fungi of grain crops to host cells or inhibit the expansion of pathogenic fungi of grain crops in host cells, thus indicating that allantoin can be used as a pesticide to prevent and treat pathogenic fungi of grain crops, especially Magnaporthe oryzae, Cochliobolus miyabeanus and Ustilaginoidea virens.

The invention discloses a method for combining straw returning to field, pond fish culture and rice, fish and soft-shelled turtle / shrimp comprehensive planting and breeding, and belongs to the technical field of rice, soft-shelled turtle, fish and shrimp comprehensive planting and breeding. After rice in the rice field is harvested, straw is smashed and returned to the field, a hidden survival place is provided for crayfish seedlings, rotten straw and derived zooplankter provide bait for crayfish and fish, and the straw decomposition rate is increased by eating; after water in the rice field module becomes serious along with straw rotting, the water is discharged into the culture pond through tail water treatment engineering treatment, and the water fertility of the pond is increased; meanwhile, after eutrophication of the water body in the culture pond, the water body is discharged into the rice field after being treated by a tail water treatment project and flows into the culture pond again after being further purified by the rice field. According to the constructed mode, the straw is converted into the organic fertilizer through the crayfish and the fish, and the paddy field is adopted for purifying pond culture tail water. No breeding wastewater is discharged in the mode. The investment of aquatic product baits and rice field fertilizers is reduced, and the income and quality of rice and fishes are increased.

The invention discloses a Ustilaginoidea virens inoculant, a preparation method and application of the Ustilaginoidea virens inoculant in Ustilaginoidea virens inoculation, and belongs to the technical field of agricultural microorganisms. According to the method, two-step shake-flask culture is combined with the steps of filtering, centrifuging and spore germination, so that the ustilaginoidea virens inoculum taking germinated spores (GC) as the core is prepared, and the problems of hypha aging, low spore activity, asynchronous infection and the like in the existing inoculation method are effectively solved. The inoculant has a tender hypha structure and strong infection activity, and the inoculation efficiency and the pathogenesis consistency are remarkably improved. When being used for preparing transcriptome samples, metabolome samples and other omics samples, the strain avoids non-specific immune interference caused by PAMPs released by broken hyphae, ensures synchronous infection process, and is real and reliable in immune response. The invention provides a stable and efficient inoculation system with a clean background for the research on the ustilaginoidea virens-rice interaction mechanism, and has a good application prospect.

The invention relates to the technical field of weed rice prevention and control, in particular to a comprehensive prevention and control method for rice field weed rice, which comprises the following steps: acquiring rice field environment and seed state data, extracting characteristics containing a multi-factor coupling relationship after processing, and inputting a weed rice dormancy-germination coupling prediction model to accurately output a germination probability and a time window. And a self-adaptive prevention and control scheme is generated and issued according to the self-adaptive prevention and control scheme and is cooperatively executed by a machine system, an irrigation system and a medicament system, and finally data feedback after execution is updated to form a closed loop. According to the method, the problem of low prevention effect caused by inaccurate germination prediction, prevention and control decision stiffness and single execution means of the weedy rice is fundamentally solved, accurate prediction of germination rules, intelligent judgment of prevention and control opportunities and cooperative control of multiple measures are realized, and the prevention and control effect and ecological economic benefits are remarkably improved.

The application discloses a reverse making method of terraced rice picture and belongs to the field of straw picture, and comprises the following steps: S1, breeding color rice varieties with different color and plant height, and carrying out planting and management according to the characteristics of terraces; S2, designing a plane pattern by using PS and AI; S3, importing the plane pattern into CAD and adjusting the angle and perspective; S4, measuring the position and range of the rice field picture area field plot and the height difference of the field ridge; S5, discharging the whole pattern in the reverse direction of the field ridge by using the Ovi interactive map, and adjusting the position and angle of the field ridge in the picture to be optimal; and S6, placing the converted pattern in the rice field by using a positioning instrument and placing and planting. The terraces are curved, the field ridge is high, the field plot surface is small, and the rice field picture cannot be made like the basin or the plain, the application adopts a reverse terraced picture making technology, the technology overcomes the disorder and the broken feeling caused by the large height difference of the terraces, the small field plot and the large bending degree, and improves the overall viewing effect of the picture.

This invention discloses a cultivated rice from Africa pi- cnv9 The application of genes in improving rice disease resistance belongs to the field of genetic engineering technology. This invention has screened a gene associated with rice blast resistance. pi-cnv9 The gene, whose nucleotide sequence is shown in SEQ ID NO.1, was used in this invention to construct complementary transgenes for *Rhizophora flavescens* and *Rhizophora esculenta*, respectively. pi-cnv9 Rice plants with complementary genes. Inoculation experiments were conducted on wild-type and transgenic plants, and the results demonstrated that complementary transgenic... pi-cnv9 Genetically modified plants are more resistant to disease than wild-type plants; indicating that... pi-cnv9 Genes that positively regulate the disease resistance of rice can be used as target genes for genetic improvement and molecular breeding of rice resistance to rice blast, which is of great significance for breeding rice varieties with improved disease resistance.

We offer a furikake (rice seasoning) product that allows you to enjoy the unique flavor of the leaves and stems of the wild rice plant. [Solution] The seasoning product 10 containing Makomo charcoal powder comprises a powdered seasoning 200 and a container body 100 for holding the seasoning. The container body has a storage section 140 for holding the seasoning, a first zipper member 162, and a second zipper member 164, and the storage section can be repeatedly sealed by interlocking the first zipper member and the second zipper member. The seasoning is a powder mixture of Makomo leaf powder, which is made by crushing dried Makomo leaves, Makomo charcoal powder, which is made by crushing carbonized Makomo stems, kelp powder, which is made by crushing dried kelp, salt koji powder, which is made by crushing dried salt koji, and salt powder.

The invention discloses a LAMP-DA3-ITS-based visual detection kit for rice flaxseed spot and application thereof, relates to the technical field of detection equipment, and aims to separate and identify rice flaxseed spot DA3 from field rice diseased leaves. The invention further develops a specific detection target DA3-ITS for rapidly and accurately identifying the rice flaxseed spot DA3. The invention further develops an LAMP primer, a kit and a detection method based on a DA3-ITS detection target. The detection method of the rice cochliobolus miyabeanus DA3, provided by the invention, has relatively high accuracy, sensitivity, effectiveness and specificity, is convenient to operate and good in practicability, and can realize accurate and rapid detection of the rice cochliobolus miyabeanus DA3. The primer pair can be used for identifying the cochliobolus miyabeanus and early detection of diseases caused by the cochliobolus miyabeanus when symptoms do not appear, and is further applied to monitoring of the distribution range of the pathogenic bacteria and the caused rice diseases, and the purpose of timely prevention is achieved.

The present invention relates to a rice seed dehulling and sorting device, and more specifically, to a rice seed dehulling and sorting device that improves work efficiency by allowing dehulling and sorting to proceed continuously by dehulling a dehulling machine for removing awns from rice seeds, and sorting chaff and husks from rice seeds from which awns have been removed through the dehulling machine, and then separating and discharging them through separate discharge lines. To this end, the present invention comprises a dehulling unit (10) for feeding rice seeds and removing awns from rice seeds, a conveying unit (20) for moving rice seeds discharged from the dehulling unit (10) to the upper side, and a sorting unit (30) for sorting and discharging rice seeds moved to the lower side through the conveying unit (20) into rice seeds, immature rice seeds, chaff, and husks. The dehulling unit (10) comprises a supply hopper (11) with an upper side open to feed rice seeds, and a falling... provided on the lower side of the supply hopper (11) The apparatus comprises a dehulling body (15) having a drive shaft and a plurality of blades inside a horizontal cylinder to remove awns from rice seeds, a discharge channel (15a) provided on the lower side of the dehulling body (15) to discharge rice seeds, and a first motor (17) mounted on a first ground support (19) provided on the lower side of the dehulling body (15) to rotate the drive shaft; the conveying unit (20) comprises a receiving hopper (21) that receives rice seeds dropped through the discharge channel (15a), a conveyor body (25) that moves rice seeds to the upper side of the receiving hopper (21), and a first hose (27) that moves rice seeds moved to the upper side through the conveyor body (25) to the lower side; and the sorting unit (30) comprises a downwardly inclined first-stage inclined body (41) that receives rice seeds fed through the first hose (27), and the It is composed of a downwardly inclined second stage inclined body (51) provided in the reverse direction at the lower end of the first stage inclined body (41), and a downwardly inclined third stage inclined body (67) provided parallel to the lower side of the second stage inclined body (51), and a first discharge path (43) for discharging rice seeds is provided at the lower end of the first stage inclined body (41).A second hose (45) for discharging chaff and husks separated from rice seeds is provided on the upper side of the first inclined body (41), a second discharge passage (52) for discharging immature rice seeds is provided on the lower side of the second inclined body (51), and a third discharge passage (59) for discharging husks is provided on the lower side of the third inclined body (67). The first discharge passage (43), the second discharge passage (52), and the third discharge passage (59) are provided on a second ground support body (31) in the form of a frame, a handle (35) is provided on the rear side of the second ground support body (31), a wheel (33) is provided on the rear lower side of the second ground support body (31), a connecting hole (21a) is vertically formed on the lower side of the side wall of the receiving hopper (21), and the passage gap of the connecting hole (21a) is adjusted. A rice seed sifting and sorting device is provided, characterized in that a spacing adjustment plate (22) is provided to slide on the upper side of a connecting hole (21a), a vertical elongated hole (22a) is formed on the spacing adjustment plate (22), and an adjustment bolt (23) is provided to be coupled to the vertical elongated hole (22a) to fix the position of the spacing adjustment plate (22).

The invention provides a nucleic acid composition and a kit for identifying a rice variety rice flower No.2, and relates to the technical field of biochemical detection. The nucleic acid composition comprises a specific amplification primer pair and a specific detection probe. The nucleic acid composition locks the specific SNP site of the rice, and by utilizing the synergistic effect of the primer and the double probes, the rice flowery No.2 variety and the similar variety can be accurately distinguished, and the doping can be sensitively identified. The method is suitable for a real-time fluorescent PCR platform, electrophoresis and sequencing are not needed, rapid and high-sensitivity closed tube detection is achieved, and an efficient means is provided for authenticity identification and purity analysis.

The invention discloses a common wild rice OrLRR1 gene and application thereof in improving rice blast resistance of rice, and belongs to the technical field of genetic engineering and phytopathology. The nucleotide sequence of the oryza vulgaris OrLRR1 gene is as shown in SEQ ID NO: 1. It is found that the OrLRR1 gene plays an important role in rice blast resistance of rice. The deletion of the OrLRR1 gene reduces the resistance of the rice to the magnaporthe oryzae, and the over-expression of the OrLRR1 gene can improve the resistance of the rice to the magnaporthe oryzae. The invention provides an application of an OrLRR1 gene or an encoding protein thereof in improving the resistance of rice to rice blast, and a method for cultivating rice blast-resistant rice by regulating and controlling the expression of OrLRR1. The invention provides a new gene resource and a theoretical basis for cultivating a new rice variety with high rice blast resistance, and has important application value.

The utility model relates to the technical field of wild rice storage devices, and discloses an ex-situ storage device for oryza meyeriana, which comprises a base, a sliding plate is slidably connected to the inner wall of the base, a spring is fixedly connected to the outer wall of the sliding plate, and a hollow frame is fixedly connected to the outer wall of the spring. The outer wall of the hollow frame is fixedly connected to the inner wall of the base, the outer wall of the sliding plate is slidably connected to the inner wall of the hollow frame, the outer wall of the sliding plate is fixedly connected with a sliding column, the outer wall of the sliding column is slidably connected to the inner wall of the hollow frame, and the outer wall of the sliding column is fixedly connected with a clamping block. And a fixing block is arranged on the outer wall of the clamping block. According to the flowerpot, the sliding plates are pushed to extrude the springs and drive the sliding columns to slide, then the clamping blocks are driven to slide out of the inner walls of the fixing blocks, locking of the fixing blocks is relieved, the flowerpot wall can be detached from the base, a user can conveniently clean rotten or diseased soil, and the effects of facilitating cleaning and maintenance and enhancing plant protection are achieved.

To provide a brown rice quality improving agent and a method for improving quality of brown rice, with which brown rice with high quality can be provided. The brown rice quality improving agent of the present invention contains zerumbone as an active ingredient. The method for improving quality of brown rice of the present invention has a step of applying zerumbone to rice plants in the cultivation period.

According to the rice area small and micro wetland nitrogen reduction potential evaluation and area grading control method and system, firstly, relevant data are collected, nitrogen reduction potential evaluation indexes of rice area small and micro wetlands are calculated, and the nitrogen reduction potential evaluation indexes comprise the nitrogen removal rate, the nitrogen removal amount and the nitrogen removal economic benefits. Then, based on the nitrogen removal amount of the small and micro wetlands in the rice area, the small and micro wetlands in different areas in the rice area are subjected to graded management and control in combination with the rice field nitrogen loads in all the areas in the rice area. The system evaluates the nitrogen reduction potential of the small and micro wetlands, fully considers biochemical factors having important influence on nitrogen removal, such as large aquatic plants, temperature, nitrogen concentration and the like, provides more reliable nitrogen reduction efficiency prediction for the actual management of the small and micro wetlands in the rice area, improves the accuracy and applicability of the evaluation model, and has good application prospects. And hierarchical management and control are carried out according to regional characteristics, so that scientific formulation and accurate implementation of protection and management measures of the small and micro wetlands in the rice area are facilitated.

The invention relates to the technical field of agriculture, and particularly provides a specific primer pair for detecting a rice salt-tolerant gene HKT1-W and application of the specific primer pair. The method comprises the following steps: selecting leaves of rice, extracting genome DNA (deoxyribonucleic acid) of the tissues, and respectively carrying out PCR (polymerase chain reaction) amplification by taking the DNA of the tissues as a template and taking corresponding sequences of YDH-F and YDH-R as specific primers to obtain amplified fragments; after electrophoresis of the amplified fragment, the single plant with only one 300bp main band is a salt-tolerant single plant, and the single plant without a band is a salt-sensitive single plant. According to the invention, the rice variety improved by using the wild rice HKT1-W gene can be rapidly bred, the current situations of much field work and large workload caused by performing a large amount of salt tolerance identification by using population materials can be improved, and the method has important theoretical and practical significance for cultivating a new variety of rice with salt tolerance character.

The application discloses an endophytic fungus LH001 and application thereof, and belongs to the technical field of plant disease control. The endophytic fungus LH001 is separated from the root system of wild rice, is classified and named as Pseudophialophora sp. LH001, is preserved in the China Center for Type Culture Collection, and has a preservation number of CCTCC NO: M 20241751. The endophytic fungus LH001 strain is co-cultured with rice, is allowed to colonize the root of rice seedlings, can reduce the leaf damage caused by Magnaporthe oryzae, enhances the disease resistance of rice seedlings to leaf blight, and the control effect reaches 94.05%. Therefore, the biological control effect of the endophytic fungus LH001 on rice blast has great value in the popularization and application in the agricultural field.

The invention discloses application of a rice OsBZR4 gene in cultivation of rice embryo-free seeds, relates to the technical field of gene engineering, and aims to provide application of the rice OsBZR4 gene in cultivation of rice embryo-free seeds, which has important practical significance and agricultural utilization value. A CRISPR (clustered regularly interspaced short palindromic repeats) / Cas9 (CRISPR associated 9) technology is utilized to perform targeted knockout on the rice gene OsBZR4, so as to obtain the rice gene OsBZR4 knockout According to the gene knockout mutant of the rice OsBZR4 gene under the background of different rice varieties, 60-90% of non-embryo seeds and 10-40% of small embryo and normal embryo seeds can be generated. On the premise that the yield and the quality character are not affected, the polished rice rate of the rice can be increased, and the storage resistance of the rice can be improved. Precious germplasm resources are provided for rice seed embryo and endosperm development research, and the method has very important scientific value for research of a rice seed embryo and endosperm development regulation mechanism.

This invention discloses an InDel marker, Pmct3, for cold tolerance during rice budding, along with its identification method and applications, belonging to the field of molecular marker technology. This invention develops an InDel molecular marker from the insertion / deletion sequence between the Dongxiang wild rice introgression line D2 (background of the cold-tolerant rice 9311) and the cold-sensitive rice 9311 at the Chr1:28249918 and Chr1:28249919 sites in the O. sativa reference genome. Primers are then developed based on this molecular marker for identifying cold tolerance during rice budding. The detection cycle of this invention is only 2-3 hours, does not rely on subjective experience in traditional detection methods, and can obtain accurate and objective detection and identification results in a short time. It is low-cost, high-throughput, and highly specific, making it suitable for rice breeding and production practices.

The utility model discloses a fish, soft-shelled turtle and rice symbiotic system which comprises a breeding base, an inverted trapezoidal plate is fixedly connected to the center of the top of the breeding base, a soil layer is arranged at the bottom in the inverted trapezoidal plate, a dirt collecting box is fixedly connected to the position, close to the bottom, in the breeding base, and a purifying box is fixedly installed at the bottom of one side of the breeding base. A first pump body is fixedly installed on one side of the bottom in the sewage collecting box, the output end of the top of the first pump body communicates with a sewage discharging pipe, one side of the sewage discharging pipe penetrates into the purifying box and extends to the top in the purifying box, and an inner side plate is fixedly connected to the center of one side of the inner wall of the purifying box. According to the fish, soft-shelled turtle and rice symbiotic system, pollutants such as excrement and residual feed generated in the breeding process are collected through the sewage collection tank, the pollutants are prevented from being directly deposited in the breeding environment, water quality cleanliness is effectively maintained, the first pump body is matched with the sewage discharge pipe to pump the pollutants in the sewage collection tank to the top of the purification tank, and preliminary separation and transfer of the pollutants are achieved.

The invention provides a perennial rice seed-fertilizer-pesticide integrated paper film direct seeding method suitable for a terrace environment, and relates to the technical field of modern agricultural direct seeding planting. The perennial rice'seed-fertilizer-pesticide 'integrated paper film direct seeding method suitable for the terraced field environment specifically comprises the following steps of S1, integrated direct seeding paper film preparation, S2, terraced field preparation and paper film laying, and S3, field management. Aiming at the pain points of complex terrace terrain, difficulty in mechanization, high labor cost, low utilization rate of water, fertilizer and pesticide and the like, the degradable integrated direct seeding paper film integrating perennial rice seeds, sustained and controlled release fertilizer and herbicide is prepared, so that seeding, base fertilizer application and weed closing are synchronously completed at one time, agricultural non-point source pollution is reduced, and the yield is increased. The yield and economic benefits of the terrace perennial rice are remarkably improved, and technical support is provided for terrace grain safety and ecological protection.

The present invention relates to the field of agricultural technology, and specifically provides a CAPS molecular marker for the salt-tolerant ST4 promoter region of rice and its application. Rice leaves are selected to extract the genomic DNA of the above-mentioned tissue, and then the DNA of the above-mentioned tissue is used as a template, and the corresponding sequences of ST4-PRO-F1 and ST4-PRO-R1 are used as specific primers to perform PCR amplification respectively to obtain amplified fragments; the amplified fragments are digested with Eco81I restriction endonuclease, and electrophoresis is performed after digestion; after electrophoresis of the ST4 promoter fragment, only one 2162bp main band is present for salt-tolerant individual plants, while two bands of 1366bp and 784bp appear in the electrophoresis for salt-sensitive individual plants. The present invention can quickly breed rice varieties improved by using the wild rice ST4 gene, which helps to improve the current situation of much field work and heavy workload caused by using group materials for a large amount of salt-tolerant identification, and has important theoretical and practical significance for breeding new rice varieties with salt-tolerant traits.

The application discloses a plant growth regulator and application thereof in relieving imazamox herbicide injury to rice. The main components of the plant growth regulator include leucine, isoleucine and valine, and the plant growth regulator is combined with imazamox in a certain application mode and proportion, and is sprayed on rice seedling leaves, so that the effect of relieving imazamox herbicide injury is better than that of the commonly used protective agent, namely, bihuhu or brassinolide on the market, and the herbicide effect of the herbicide is not affected. The application also clearly defines the application conditions of the safety agent, and is a plant growth regulator which can effectively relieve imazamox herbicide injury to rice and does not reduce the herbicide effect on weeds.

The invention relates to the field of cosmetics, in particular to a skin care composition capable of resisting environmental pollution. The skin care composition comprises main functional components such as a mixture of a rice germ extract and a rice extract, a mixture of a chondrus crispus extract and a lithocladus calcaratus extract, a nannochloropsis oculata extract, a mixture of olive fruit water and an olive fruit extract and the like. The composition can isolate air pollutants, inhibit the air pollutants from adhering to sebum, prevent the air pollutants from invading into skin, and reduce the size and number of facial pores to effectively reduce sebum secretion; the tolerance of skin to irritants is improved, the oxidation resistance is improved, and the probability of inflammation caused by sebum peroxidation and the like is reduced. The cell viability is improved, so that pollutants in the air are resisted.