157 results about "Oryza" patented technology

The embodiment of the invention provides a rice blast disease grade intelligent evaluation and disease resistance preliminary screening method, system and equipment, and the method comprises the steps: S1, collecting image data of wild rice leaves infected with rice blast, carrying out the instance segmentation of the image data through an improved YOLOv8-Seg segmentation model, obtaining a segmentation result, and carrying out the segmentation of the image data through an improved YOLOv8-Seg segmentation model; the improvement measures comprise a GhostHierachicalNet backbone network, a CAHSFPN neck network and an LSDES Head segmentation head, and the LSDES Head segmentation head is connected with the CAHSFPN neck network and the LSDES Head segmentation head; s2, according to the segmentation result, counting the number and proportion of pixels of diseased leaves and disease areas, and obtaining a disease grade evaluation result in combination with disease resistance identification technical regulations; and S3, screening wild rice materials with strong disease resistance based on the disease grade evaluation results which are repeated for multiple times. The method can effectively improve the accuracy and efficiency of crop disease evaluation, and provides support for large-scale screening of disease resistance of wild rice, thereby promoting the development of rice blast-resistant breeding work.

The invention discloses a cladosporium sp. Fungus YS37 and application thereof in promoting rice growth, and belongs to the technical field of microorganisms. The cladosporioides sp. Fungus YS37 is a new species separated from the root system of wild rice, is named as Parasarocladium oryzae YS37, is preserved in the China Center for Type Culture Collection (CCTCC), and has a preservation number of CCTCC NO: M 20242286, and the cladosporioides sp. Fungus YS37 is a new species which is separated from the root system of wild rice and is named as Parasarocladium oryzae YS37. The Parasarocladium oryzae YS37 strain can be used for preparing a biological bacterial fertilizer, and when the Parasarocladium oryzae YS37 strain or a fermentation product of the Parasarocladium oryzae YS37 strain is applied to a rice root system, the growth of rice can be effectively promoted, and the plant biomass is increased. The invention provides a new way for application of microorganisms to development of crop production.

The invention discloses an endophytic fungus LH001 and application thereof, and belongs to the technical field of plant disease control. The endophytic fungus LH001 is separated from the root system of wild rice, is classified and named as Pseudophialophora sp.LH001, is preserved in the China Center for Type Culture Collection (CCTCC), and has the preservation number of CCTCC NO: M 20241751. The endophytic fungus LH001 strain is co-cultured with rice and colonized at the root of a rice seedling, so that leaf harm caused by magnaporthe oryzae can be reduced, the disease resistance of the rice to leaf blast in the seedling stage is enhanced, and the prevention and treatment effect reaches 94.05%. Therefore, the endophytic fungus LH001 has great value in popularization and application of the biological control effect on the rice blast in the agricultural field.

The invention discloses an application of a oryza longistaminata OlARF9 gene in improving hypoxia stress resistance of rice, and belongs to the field of bioengineering. According to the invention, overexpression is carried out on the oryza longistaminata OlARF9 gene in rice by adopting an overexpression technology, and according to phenotypic changes of transgenic plants, overexpression of the oryza longistaminata OlARF9 gene in the rice can significantly improve the ability of the rice to resist hypoxia stress, so that the oryza longistaminata OlARF9 gene can be applied to improve the resistance of the rice to abiotic stress and improve the resistance of the oryza longistaminata OlARF9 gene in the rice. Comprising the following steps: overexpressing the oryza longistaminata OlARF9 gene in rice to obtain a transgenic plant, wherein the resistance of the obtained transgenic plant to hypoxia stress is obviously enhanced. The method has application prospects in the aspects of improving and enhancing rice stress resistance, accelerating the stress-resistant molecular breeding process and the like.

The invention discloses application of a rice OsbHLH083 gene in regulation and control of seed germination capability, and relates to the technical field of plant genetic engineering. The CDS sequence of the rice OsbHLH083 gene is as shown in SEQ ID NO. 2, and the amino acid sequence of the coded protein is as shown in SEQ ID NO. 3. The molecular biology function of the rice OsbHLH083 gene for regulating rice germination is studied, after the rice OsbHLH083 gene is edited through CRISPR / Cas9, compared with a wild type, the rice OsbHLH083 gene mutant rice seeds germinate slower, the rice OsbHLH083 gene and a protein coded by the rice OsbHLH083 gene can be used for regulating the phenotype of seed germination, and the rice OsbHLH083 gene mutant rice seeds germinate more slowly. And a gene resource is provided for the genetic improvement of rapid germination and seedling formation characters of the direct seeding rice seeds in the field.

The invention relates to the technical field of plant genetic engineering, and discloses a haplotype identification marker of a rice flood-resistant gene ARF9 and application of the haplotype identification marker. The molecular marker is derived from an exon of an ARF9 gene, is located at the 22009082bp and 22010019bp of a No.4 chromosome of rice, and has a basic group of C. The SNP molecular marker is related to the waterflooding resistance of a male parent oryza longistaminate of perennial rice, and the waterflooding resistance of the male parent oryza longistaminate with a locus genotype of CC is obviously higher than that of rice with a locus genotype of non-AA type. Therefore, the invention provides the loop-mediated isothermal amplification primer group for rapidly identifying the ARF9 gene haploid molecular marker, and the loop-mediated isothermal amplification primer group is used for identifying the ARF9 gene haploid molecular marker so as to rapidly screen out waterflooding-resistant rice. The invention provides a group of primers, the flood-resistant rice can be quickly identified by adopting the primers to perform loop isothermal reaction, the detection cost is low, the detection speed is high, the flood-resistant germplasm can be quickly screened in the rice seedling stage by adopting the method provided by the invention, a time-consuming flooding test does not need to be performed in the later stage, and the breeding process is greatly accelerated.

The invention discloses application of rice protein MBS and a coding gene thereof in plant light protection. The invention provides any one of the following applications of the protein OsMBS. C1, regulating and controlling the plant yield; c2, regulating and controlling light protection of the plants; c3, regulating and controlling the strong light adaptability of the plants; c4, regulating and controlling the photosynthetic activity of the plants; and C5, cultivating a strong light resistant plant. Transgenosis experiments and field yield measurement verify that the homologous protein OsMBS has enhanced expression in rice, the chloroplast is wrapped in a sharp increase mode under strong light, a shading layer for directly protecting the chloroplast is formed, light protection of the rice fragrance of the rice can be enhanced, and remarkable and efficient yield increase in the field is achieved.

The invention discloses an application of a FopstS gene in regulation and control of endophytic fungi viosphaeria fusarium to promote rice growth, and belongs to the technical field of gene engineering. The nucleotide sequence of the FopstS gene is as shown in SEQ ID NO. 1. The invention discloses the gene FopstS for regulating and controlling the growth of the rice in the rice endophytic fungus Bottle fusarium oxysporum for the first time, the gene FopstS is cloned and analyzed, and functional verification is performed on the gene by utilizing gene knockout and gene overexpression technologies, and the fact that the growth promoting property of a strain on the rice is obviously reduced due to the fact that the gene FopstS is knocked out is found, so that the growth promoting property of the strain on the rice is obviously reduced, and the growth promoting property of the strain on the rice is obviously improved. The overexpression of the FopstS gene leads to the substantial enhancement of the growth promoting property of the strain on the rice, and the research result has huge application potential in sustainable agriculture, and provides a research thought for improving the rice yield and reducing the use of chemical fertilizers.

Disclosed are: an improved Oryza plant, or a plant portion thereof, having two copies of a gene encoding OsNramp5 protein, and a method for producing an improved Oryza plant. The method and method for producing an improved Oryza plant comprises: (I) a step for crossing Pokkali with an Oryza plant other than Pokkali to obtain an F1 individual; (II) a step for bringing about self-fertilization of the F1 individual to obtain an F2 individual; and (A) a step for confirming that the obtained F2 individual has two copies of a gene encoding OsNramp5 protein. The method also comprises: (I) a step for crossing Pokkali with an Oryza plant other than Pokkali to obtain an F1 individual; (II') as necessary a step for bringing about self-fertilization of the F1 individual to obtain an F2 individual; (III) a step for backcrossing the F1 individual or F2 individual with an Oryza plant other than Pokkali to obtain a BC1F1 individual; and (IV) as necessary a step for further repeating steps (II') to (III) n-1 times using the BC1F1 individual to obtain a BCnF1 individual (n represents an integer of 2 or more), wherein the method includes (A) a step for confirming that an individual obtained in at least one step selected from the group consisting of steps (I)-(IV) has two copies of a gene encoding OsNramp5 protein.

The invention relates to the technical field of biology, and particularly provides an InDel molecular marker for screening a rice salt-tolerant gene Ts33 and application of the InDel molecular marker. The method comprises the following steps: selecting leaves of rice to extract genome DNA (deoxyribonucleic acid) of the tissues, then respectively carrying out PCR (polymerase chain reaction) amplification by taking the DNA of the tissues as a template and taking corresponding sequences of ProTs33-F1 and ProTs33-R1 as specific primers to obtain amplified fragments, and after electrophoresis, determining that the strains with 238bp bands are salt-sensitive strains, and determining that the strains without bands are salt-tolerant strains. According to the invention, the rice variety improved by utilizing the wild rice Ts33 gene can be rapidly bred, the current situations of more field work and large workload caused by carrying out a large amount of salt tolerance identification by utilizing a population material can be improved, and the method has important theoretical and practical significance for breeding a new variety of rice with salt tolerance character.

The invention provides application of Ustilaginoidea virens secretory protein UvWMT and a coding gene thereof in prevention and control of plant diseases. The gene sequence of the Ustilaginoidea virens secretory protein UvWMT is researched, found and cloned for the first time, and the new application of the Ustilaginoidea virens secretory protein UvWMT in disease-resistant breeding of crops such as rice is developed. Research finds that the protein is a key pathogenic factor in the process that rice is infected by ustilaginoidea virens, heterologous expression experiments further find that heterologous expression of UvWMT in rice can significantly improve the resistance of crops to various pathogenic bacteria such as ustilaginoidea virens, magnaporthe oryzae and xanthomonas oryzae, and has no significant difference in key agronomic traits compared with a wild type. An important theoretical basis is provided for rice disease-resistant breeding, crops can be safely and effectively endowed with broad-spectrum disease resistance by heterologous expression of the gene, and the gene has great application prospects and economic values in the aspects of crop breeding disease resistance improvement, rice disease prevention and control and crop yield increase and pesticide reduction.

The invention provides an application of a Ustilaginoidea virens UvWSC1 gene in improvement of plant disease resistance. The invention develops a new application of the Ustilaginoidea virens UvWSC1 gene in improving the broad-spectrum disease resistance of crops such as rice for the first time, and the UvWSC1 gene is subjected to heterologous high expression in the rice, so that the broad-spectrum resistance of the rice to various pathogenic bacteria such as Ustilaginoidea virens, Magnaporthe oryzae and Xanthomonas oryzae can be obviously enhanced, and the agronomic traits of the rice are not influenced; an important theoretical basis is provided for rice disease-resistant breeding. By heterologous expression of the UvWSC1 gene, broad-spectrum disease resistance can be safely and effectively endowed to crops, a broad-spectrum disease-resistant rice variety is obtained for production, and the broad-spectrum disease-resistant rice gene has great application prospects and economic values in the aspects of crop breeding disease resistance improvement, rice disease prevention and control and crop yield increase and pesticide reduction.

The invention relates to the technical field of plant molecular breeding, in particular to application of PH8 protein or a coding gene thereof in regulation of plant height, ear length or lodging resistance of plants. The PH8 protein is cytochrome P450 CYP78A15, and the plant is an oryza plant. The PH8 protein comprises an amino acid sequence as shown in SEQ ID NO.1. The application comprises the following steps: reducing the expression level of the PH8 protein in the plant, reducing the plant height and spike length of the plant, and improving the lodging resistance of the plant. The PH8 protein related to the plant height of the oryza plant is obtained through screening, and the plant height and the ear length of the oryza plant can be regulated and controlled by regulating and controlling the expression level of the PH8 protein in the oryza plant. The PH8 protein provided by the invention can be used for cultivating oryza plants with strong lodging resistance, does not influence the yield of the oryza plants, and has important application value.

The invention discloses a method for in-vitro preservation of common wild rice and Nwa wild rice, and belongs to the technical field of plant tissue culture. The method comprises the following steps: selecting a stem node with an axillary bud as an explant; soaking in 75% alcohol for 10-15 seconds, soaking in 10% 84 disinfectant for 15 minutes, performing vacuum treatment in 0.1% mercuric chloride solution under-0.1 Mpa for 5 minutes, and performing water bath at 40 DEG C for 20 minutes for combined sterilization; inserting the sterilized explant into a bud differentiation culture medium containing 2mg / L KT and 0.1 mg / L NAA, and culturing for 3-5 days under the conditions that the temperature is 23 DEG C and illumination is performed for 16 hours per day; cutting off buds growing to 3-5cm, and transferring the buds into a rooting culture medium; (5) carrying out test-tube plantlet propagation through stem node shearing or tillering cutting; the tissue culture seedlings with well developed root systems are hardened and then transplanted to a mixture of vermiculite and nutrient soil (v / v = 1 / 1). According to the method, the pollution rate is as low as 16.35%, the differentiation rate of the explant reaches 47.36%, the process is simple, convenient and efficient, the production period is short, the seed quality resources of common wild rice and Nilval wild rice can be effectively preserved, and the method has important significance on protection of precious wild rice resources.

The invention relates to the technical field of biological genes, in particular to clone of an oryza brachytonkinensis gene SBPL6 and application of the oryza brachytonkinensis gene SBPL6 to improvement of stigma traits of a rice male sterile line, in particular to application of the oryza brachytonkinensis gene SBPL6 to improvement of stigma traits of a sterile line, and improvement of the stigma traits of the sterile line is beneficial to improvement of outcrossing ability of the sterile line. The cDNA (complementary deoxyribonucleic acid) sequence of the gene SBPL6 is shown as SEQ ID NO.2. The wild brachyglossum gene SBPL6 can effectively increase the hair brush length and the stigma length of a rice stigma, so that the stigma exsertion rate is increased, and the outcrossing capability of a sterile line is favorably improved.

The invention relates to the technical field of agriculture, and particularly provides a CAPS molecular marker of a rice salt-tolerant ST4 promoter region and application of the CAPS molecular marker. The method comprises the following steps: selecting leaves of rice to extract genome DNA (Deoxyribonucleic Acid) of the tissues, and then respectively carrying out PCR (Polymerase Chain Reaction) amplification by taking the DNA of the tissues as a template and taking corresponding sequences of ST4-PRO-F1 and ST4-PRO-R1 as specific primers to obtain amplified fragments; carrying out enzyme digestion on the amplified fragment by using Eco81I restriction enzyme, and carrying out electrophoresis after enzyme digestion; after the ST4 promoter fragment is subjected to electrophoresis, a single plant with only one 2162bp main band is a salt-tolerant single plant, and a single plant with 1366bp and 784bp bands appearing in electrophoresis is a salt-sensitive single plant. According to the invention, the rice variety improved by utilizing the wild rice ST4 gene can be rapidly bred, the current situations of much field work and large workload caused by carrying out a large amount of salt tolerance identification by utilizing a population material can be improved, and the method has important theoretical and practical significance for breeding a new variety of rice with salt tolerance character.

The present application relates to the application of allantoin in the prevention and treatment of fungal diseases of grain crops. The present application finds that allantoin can inhibit the growth of pathogenic fungi of grain crops, especially inhibit the infection of pathogenic fungi of grain crops to host cells or inhibit the expansion of pathogenic fungi of grain crops in host cells, thus indicating that allantoin can be used as a pesticide to prevent and treat pathogenic fungi of grain crops, especially Magnaporthe oryzae, Cochliobolus miyabeanus and Ustilaginoidea virens.

The present invention relates to the field of rice genetic engineering and discloses the application of the rice orphan gene DUD1 in controlling plant drought resistance. The applicant isolated, cloned and functionally verified a rice orphan gene that can enhance rice drought resistance. DUD1 (

The invention discloses a method for combining straw returning to field, pond fish culture and rice, fish and soft-shelled turtle / shrimp comprehensive planting and breeding, and belongs to the technical field of rice, soft-shelled turtle, fish and shrimp comprehensive planting and breeding. After rice in the rice field is harvested, straw is smashed and returned to the field, a hidden survival place is provided for crayfish seedlings, rotten straw and derived zooplankter provide bait for crayfish and fish, and the straw decomposition rate is increased by eating; after water in the rice field module becomes serious along with straw rotting, the water is discharged into the culture pond through tail water treatment engineering treatment, and the water fertility of the pond is increased; meanwhile, after eutrophication of the water body in the culture pond, the water body is discharged into the rice field after being treated by a tail water treatment project and flows into the culture pond again after being further purified by the rice field. According to the constructed mode, the straw is converted into the organic fertilizer through the crayfish and the fish, and the paddy field is adopted for purifying pond culture tail water. No breeding wastewater is discharged in the mode. The investment of aquatic product baits and rice field fertilizers is reduced, and the income and quality of rice and fishes are increased.

The invention discloses a method for transplanting perennial rice seedlings. The method comprises the following steps: releasing water before harvesting, leaving a 25-30 cm pile, and timely covering with water to a depth of 20 cm; maintaining a field water depth of 20 cm during winter management; lowering the water level to 10 cm when seedlings emerge in March of the following year to promote the germination of new buds, and then covering with water to 20 cm; applying fertilizer to promote seedling growth in early April; and pulling out seedlings for transplanting in late April. The method can successfully overwinter under flooding conditions, and the winter survival rate in the second year can reach about 98%. Moreover, according to different flooding depths, the seedling positions in the second year are also different. Under deep flooding conditions, the seedlings emerging from the elongated internodes of perennial rice account for more than 85% of the total number of seedlings, and the seedlings emerging from underground stems account for less than 15%, which provides favorable conditions for pulling out and transplanting perennial rice seedlings. At the same time, the first year of perennial rice can be grown in a nursery field with dense planting for the second year. Through seedling separation and transplanting technology, one acre of seedlings grown in the next year can be transplanted to about 10 acres of the field. At the same time, there is no need to raise seedlings, early seedlings, early transplanting, and two harvests can be achieved in the second year.

The invention discloses a Ustilaginoidea virens inoculant, a preparation method and application of the Ustilaginoidea virens inoculant in Ustilaginoidea virens inoculation, and belongs to the technical field of agricultural microorganisms. According to the method, two-step shake-flask culture is combined with the steps of filtering, centrifuging and spore germination, so that the ustilaginoidea virens inoculum taking germinated spores (GC) as the core is prepared, and the problems of hypha aging, low spore activity, asynchronous infection and the like in the existing inoculation method are effectively solved. The inoculant has a tender hypha structure and strong infection activity, and the inoculation efficiency and the pathogenesis consistency are remarkably improved. When being used for preparing transcriptome samples, metabolome samples and other omics samples, the strain avoids non-specific immune interference caused by PAMPs released by broken hyphae, ensures synchronous infection process, and is real and reliable in immune response. The invention provides a stable and efficient inoculation system with a clean background for the research on the ustilaginoidea virens-rice interaction mechanism, and has a good application prospect.

The invention relates to the technical field of weed rice prevention and control, in particular to a comprehensive prevention and control method for rice field weed rice, which comprises the following steps: acquiring rice field environment and seed state data, extracting characteristics containing a multi-factor coupling relationship after processing, and inputting a weed rice dormancy-germination coupling prediction model to accurately output a germination probability and a time window. And a self-adaptive prevention and control scheme is generated and issued according to the self-adaptive prevention and control scheme and is cooperatively executed by a machine system, an irrigation system and a medicament system, and finally data feedback after execution is updated to form a closed loop. According to the method, the problem of low prevention effect caused by inaccurate germination prediction, prevention and control decision stiffness and single execution means of the weedy rice is fundamentally solved, accurate prediction of germination rules, intelligent judgment of prevention and control opportunities and cooperative control of multiple measures are realized, and the prevention and control effect and ecological economic benefits are remarkably improved.

The application discloses a reverse making method of terraced rice picture and belongs to the field of straw picture, and comprises the following steps: S1, breeding color rice varieties with different color and plant height, and carrying out planting and management according to the characteristics of terraces; S2, designing a plane pattern by using PS and AI; S3, importing the plane pattern into CAD and adjusting the angle and perspective; S4, measuring the position and range of the rice field picture area field plot and the height difference of the field ridge; S5, discharging the whole pattern in the reverse direction of the field ridge by using the Ovi interactive map, and adjusting the position and angle of the field ridge in the picture to be optimal; and S6, placing the converted pattern in the rice field by using a positioning instrument and placing and planting. The terraces are curved, the field ridge is high, the field plot surface is small, and the rice field picture cannot be made like the basin or the plain, the application adopts a reverse terraced picture making technology, the technology overcomes the disorder and the broken feeling caused by the large height difference of the terraces, the small field plot and the large bending degree, and improves the overall viewing effect of the picture.

This invention discloses a cultivated rice from Africa pi- cnv9 The application of genes in improving rice disease resistance belongs to the field of genetic engineering technology. This invention has screened a gene associated with rice blast resistance. pi-cnv9 The gene, whose nucleotide sequence is shown in SEQ ID NO.1, was used in this invention to construct complementary transgenes for *Rhizophora flavescens* and *Rhizophora esculenta*, respectively. pi-cnv9 Rice plants with complementary genes. Inoculation experiments were conducted on wild-type and transgenic plants, and the results demonstrated that complementary transgenic... pi-cnv9 Genetically modified plants are more resistant to disease than wild-type plants; indicating that... pi-cnv9 Genes that positively regulate the disease resistance of rice can be used as target genes for genetic improvement and molecular breeding of rice resistance to rice blast, which is of great significance for breeding rice varieties with improved disease resistance.

We offer a furikake (rice seasoning) product that allows you to enjoy the unique flavor of the leaves and stems of the wild rice plant. [Solution] The seasoning product 10 containing Makomo charcoal powder comprises a powdered seasoning 200 and a container body 100 for holding the seasoning. The container body has a storage section 140 for holding the seasoning, a first zipper member 162, and a second zipper member 164, and the storage section can be repeatedly sealed by interlocking the first zipper member and the second zipper member. The seasoning is a powder mixture of Makomo leaf powder, which is made by crushing dried Makomo leaves, Makomo charcoal powder, which is made by crushing carbonized Makomo stems, kelp powder, which is made by crushing dried kelp, salt koji powder, which is made by crushing dried salt koji, and salt powder.

The invention discloses a LAMP-DA3-ITS-based visual detection kit for rice flaxseed spot and application thereof, relates to the technical field of detection equipment, and aims to separate and identify rice flaxseed spot DA3 from field rice diseased leaves. The invention further develops a specific detection target DA3-ITS for rapidly and accurately identifying the rice flaxseed spot DA3. The invention further develops an LAMP primer, a kit and a detection method based on a DA3-ITS detection target. The detection method of the rice cochliobolus miyabeanus DA3, provided by the invention, has relatively high accuracy, sensitivity, effectiveness and specificity, is convenient to operate and good in practicability, and can realize accurate and rapid detection of the rice cochliobolus miyabeanus DA3. The primer pair can be used for identifying the cochliobolus miyabeanus and early detection of diseases caused by the cochliobolus miyabeanus when symptoms do not appear, and is further applied to monitoring of the distribution range of the pathogenic bacteria and the caused rice diseases, and the purpose of timely prevention is achieved.

The present invention relates to a rice seed dehulling and sorting device, and more specifically, to a rice seed dehulling and sorting device that improves work efficiency by allowing dehulling and sorting to proceed continuously by dehulling a dehulling machine for removing awns from rice seeds, and sorting chaff and husks from rice seeds from which awns have been removed through the dehulling machine, and then separating and discharging them through separate discharge lines. To this end, the present invention comprises a dehulling unit (10) for feeding rice seeds and removing awns from rice seeds, a conveying unit (20) for moving rice seeds discharged from the dehulling unit (10) to the upper side, and a sorting unit (30) for sorting and discharging rice seeds moved to the lower side through the conveying unit (20) into rice seeds, immature rice seeds, chaff, and husks. The dehulling unit (10) comprises a supply hopper (11) with an upper side open to feed rice seeds, and a falling... provided on the lower side of the supply hopper (11) The apparatus comprises a dehulling body (15) having a drive shaft and a plurality of blades inside a horizontal cylinder to remove awns from rice seeds, a discharge channel (15a) provided on the lower side of the dehulling body (15) to discharge rice seeds, and a first motor (17) mounted on a first ground support (19) provided on the lower side of the dehulling body (15) to rotate the drive shaft; the conveying unit (20) comprises a receiving hopper (21) that receives rice seeds dropped through the discharge channel (15a), a conveyor body (25) that moves rice seeds to the upper side of the receiving hopper (21), and a first hose (27) that moves rice seeds moved to the upper side through the conveyor body (25) to the lower side; and the sorting unit (30) comprises a downwardly inclined first-stage inclined body (41) that receives rice seeds fed through the first hose (27), and the It is composed of a downwardly inclined second stage inclined body (51) provided in the reverse direction at the lower end of the first stage inclined body (41), and a downwardly inclined third stage inclined body (67) provided parallel to the lower side of the second stage inclined body (51), and a first discharge path (43) for discharging rice seeds is provided at the lower end of the first stage inclined body (41).A second hose (45) for discharging chaff and husks separated from rice seeds is provided on the upper side of the first inclined body (41), a second discharge passage (52) for discharging immature rice seeds is provided on the lower side of the second inclined body (51), and a third discharge passage (59) for discharging husks is provided on the lower side of the third inclined body (67). The first discharge passage (43), the second discharge passage (52), and the third discharge passage (59) are provided on a second ground support body (31) in the form of a frame, a handle (35) is provided on the rear side of the second ground support body (31), a wheel (33) is provided on the rear lower side of the second ground support body (31), a connecting hole (21a) is vertically formed on the lower side of the side wall of the receiving hopper (21), and the passage gap of the connecting hole (21a) is adjusted. A rice seed sifting and sorting device is provided, characterized in that a spacing adjustment plate (22) is provided to slide on the upper side of a connecting hole (21a), a vertical elongated hole (22a) is formed on the spacing adjustment plate (22), and an adjustment bolt (23) is provided to be coupled to the vertical elongated hole (22a) to fix the position of the spacing adjustment plate (22).

The invention provides a nucleic acid composition and a kit for identifying a rice variety rice flower No.2, and relates to the technical field of biochemical detection. The nucleic acid composition comprises a specific amplification primer pair and a specific detection probe. The nucleic acid composition locks the specific SNP site of the rice, and by utilizing the synergistic effect of the primer and the double probes, the rice flowery No.2 variety and the similar variety can be accurately distinguished, and the doping can be sensitively identified. The method is suitable for a real-time fluorescent PCR platform, electrophoresis and sequencing are not needed, rapid and high-sensitivity closed tube detection is achieved, and an efficient means is provided for authenticity identification and purity analysis.

The invention discloses a multi-effect microbial organic compound fertilizer and a preparation method and application thereof, compared with a traditional organic fertilizer or a bio-organic fertilizer, the compound microbial agent provided by the invention is extracted from plant rhizosphere, has the functions of growth promotion and yield increase, immune induced resistance, stress adaptation and resistance and the like, and can be applied to large-scale fermentation production of the multi-effect microbial organic compound fertilizer. The compound is compounded into a fermented and decomposed organic fertilizer, is applied to commercial crops or field crops, and can achieve the effects of promoting crop growth, resisting diseases and the like; according to the invention, through combined use of the hyphae rhamnoides and the fusarium oxysporum, pathogenic bacteria can be inhibited, and root growth and nutrient absorption capability of crops can be enhanced at the same time; the combination of pseudobottle oryzae and piriformospora indica can improve the disease resistance of crops, promote the growth of the crops and increase the yield of the crops; through the synergistic effect of multiple strains, the yield and quality of crops can be improved, soil health can be effectively improved, and sustainable development of green agriculture is promoted.

The invention discloses a common wild rice OrLRR1 gene and application thereof in improving rice blast resistance of rice, and belongs to the technical field of genetic engineering and phytopathology. The nucleotide sequence of the oryza vulgaris OrLRR1 gene is as shown in SEQ ID NO: 1. It is found that the OrLRR1 gene plays an important role in rice blast resistance of rice. The deletion of the OrLRR1 gene reduces the resistance of the rice to the magnaporthe oryzae, and the over-expression of the OrLRR1 gene can improve the resistance of the rice to the magnaporthe oryzae. The invention provides an application of an OrLRR1 gene or an encoding protein thereof in improving the resistance of rice to rice blast, and a method for cultivating rice blast-resistant rice by regulating and controlling the expression of OrLRR1. The invention provides a new gene resource and a theoretical basis for cultivating a new rice variety with high rice blast resistance, and has important application value.