The invention discloses a method for obtaining germination effective symbiotic fungus of Cymbidium bicolor seeds, which includes: using dead twigs, fallen leaves, bark, moss, humus and the like around an original habitant of Cymbidium bicolor as culture medium, subjecting the culture medium and the seeds of Cymbidium bicolor to ex-site symbiotic germination to induce the seeds to germinate and generate protocorms; sterilizing the surface of each obtained protocorm, culturing with thy artificial culture medium under the sterile condition, inducing endophytic fungus in the protocorms to grow, purifying the fungus to obtain a pure colony after mycelia appears; and subjecting the obtained fungus and the seeds of Cymbidium bicolor to symbiotic germination culturing, carrying out a control experiment to check the effectiveness of the obtained fungus to germination of the seeds of Cymbidium bicolor, screening germination effective symbiotic fungus for the seeds of Cymbidium bicolor, and subjecting the fungus to molecular identification and storage. The method is simple to operate, singe variety of fungi can be obtained, massive and troublesome screening is not needed, and a new way of using Cymbidium bicolor seeds and fungus to symbiotically germinate to culture seedlings is opened.