234 results about "Huperzia serrata" patented technology

The present invention provides formulas for producing compositions for the structural / functional nutritional support for those who struggle with poor focus, concentration and / or memory. In addition, the present invention provides compositions comprising nutritional / botanical factors helpful to those who subjectively experience transient mental fatigue or poor cognitive function. The compositions of this invention consist primarily of the following ingredients B-complex vitamins, antioxidants, minerals, phosphatidyl serine (PS), choline, dimenthyl-aminoethanol (DMAE), docosahexaenoic acid (DHA), L-pyroglutamic acid, as well as herbal extracts from Bacopa monniera, Vinca minor, and Huperzia serrata. The present invention also relates to the administration of these compounds to alleviate mental fatigue or poor cognitive function.

The invention relates to a drug for treating senile postoperative acute mental disorders. The drug is prepared from the following substances in proportion by weight: common alstonia bark leaves, herba selaginellae, ophicslcite ophicalcitum, fagopyrum cymosum, fiveleaf akebia fruits, euryale ferox, pummelo peel, celosia cristata, stalactite, folium polygoni tnctorii, seeds of Chinese dodder, grand torreya seeds, litchi, dyosma difformis, eucommia ulmoides, exocarpium benincasae, peucedanum decursivum maxim, tuber fleeceflower stems, chingma abutilon seeds, longan pulp, semen lepidii, soybean germinated, biotite schist and mica, valeriana jatamansi jones, saxifraga stolonifera, spina gleditsiae, folium turpiniae, Chinese honey locust, glechoma longituba, nodular branches of pine, adonis, impatiens balsamina, caulis bambusae in taeniam, desert asparagus, murraya paniculata, saururus chinensis, string beans, leaves of purpleflower holly, veronicastrum herb, Chinese starjasmine stems, abalone, lysionotius pauciflorus maxim, radix ampelopsis, radix scrophulariae, microcos paniculata, dayflowers, fructus piperis longi, corn stigmas, huperzia serrata and amethyst. The drug for treating senile postoperative acute mental disorders disclosed by the invention is prepared from natural medicines, has a clear curative effect, can be used for effectively treating the senile postoperative acute mental disorders, and therefore, the drug is very suitable for clinical application.

The invention discloses a huperzia serrata endophytic fungus for producing huperzine A, and belongs to the technical field of biological engineering. The fungus is obtained by separating from fern H. serrata living plant according to the endophytic fungus separation and purification technology, named as shiraia sp. slf-14 by microbial taxonomy authentication and collected in CCTCC on Nov. 6th, 2009 with the collection number of CCTCC No: M 209294. The strain can produce the huperzine A by liquid fermentation. The invention discovers the huperzine A contained in the shiraia sp. fermentation liquid for the first time, and provides a new path for seeking an active huperzine A component resource for treating senile dementia.

The invention discloses a method for inducing and multiplying a prothallium of Huperzia serrata. The method comprises the following steps of taking a sporangium or a spore as an explant, performing in vitro tissue culturing on the explant; establishing an induced system of the prothallium of the Huperzia serrata, wherein the induction frequency of the prothallium reaches 15 percent; and under an artificial regulation, performing multiplication culturing for the prothallium until the month multiplication time reaches 5 times. The method is easy, has high efficiency, and can be used for establishing an import technical base for artificial propagation for the Huperzia serrata.

The invention discloses endophytic fungi from huperzia serrata. The invention is obtained through being separated from the living body of pteridophyta huperzia serrata by adopting endophytic fungi separation and purification technology, and is identified through biosystematics to be acremonium endophytium. The preservation number of the strain is CCTCC M 206118. The invention generates Huperzine A analogous compound through the strain liquid fermentation of the endophytic fungi from huperzia serrata, and is significant micro organism for searching the Huperzine A new resource of the active component for curing the presenile dementia.

The invention relates to a method for extracting and separating huperzine A and huperzine B from huperzia serrata, which comprises total huperzine extraction and purification, and huperzine A / huperzine B separation and crystallization. The invention has the following advantages: acid soaking and ultrasonic repeated extraction can sufficiently precipitate total huperzines in the huperzia serrata; activated carbon and cation exchange resin are utilized to remove impurities; reversion phase chromatography columns are utilized to separate huperzine A and huperzine B; and concentration crystallization and recrystallization are respectively utilized to enhance the purity of the huperzine A and huperzine B. The invention effectively utilizes natural resources, enhances the recovery rate and extract content, and improves the economic benefit.

The invention relates to plant endophytic fungi of huperzia serrata. The biological classification and molecular biological identification are (Trichodermasp.) L44. The bacterium has been already preserved in China Center for Type Culture Collection, with the preserving number of CCTCC NO: M2013198. The invention further provides an application of the plant endophytic fungi of huperzia serrata in preparing huperzine A. The invention provides a novel microorganism for source of the huperzine A. The microorganism provided by the invention has the advantages of quick growth and reproduction, no limitation on external environments such as seasons and climates and large yield promoting space and the like. The wild resource of the medicinal plant huperzia serrata can be effectively prevented from being excessively excavated.

A method for extracting huperzine a from huperzia serrata comprises procedures including dilute acid soaking, repeated ultrasonic assisted extraction, repeated dilute acid and dilute alkali extraction, impurity removal by the aid of activated carbon, multiple times of low temperature recrystallization, and the like. The method has the advantages that alkaloid can be converted into alkaloid salt so as to be dissolved out by means of the dilute acid soaking procedure, cell walls of the huperzia serrata can be broken by means of the ultrasonic assisted extraction procedure in an extracting process, so that the alkaloid can be dissolved into soaking liquid better, and recovery rate is improved. Upon testing, the recovery rate of the huperzine a extracted by the method can reach 98.3%. Dilute acid and dilute alkali are used for extracting and re-extracting, so that impurities can be removed well and then are further removed better by the activated carbon, finally, residual impurities are removed by means of the repeated low temperature recrystallization procedure, and accordingly the high-purity huperzine a can be obtained. An embodiment detects that the purity of the extracted huperzine a reaches 99.11% HPLC (high performance liquid chromatography).

The invention belongs to the technical fields of medicinal plants and agricultural microorganism and particularly relates to an endophytic fungi which is separated from a medicinal plant huperzia serrata, can generate huperzine a or analogues, and can be used in mass production of the huperzine a or analogues. The separated endophytic fungi ES026 is identified as colletotrichum gloeosporioides bymolecular biology and morphology, and the strain has been preserved in China Center For Type Culture Collection (CCTCC), with the preservation number of CCTCC NO: M2011046. The endophytic fungi is significantly characterized in that after liquid fermentation, the strain can generate an active substance with reversibility which can inhibit acetylcholinesterase, and high-performance liquid chromatography and mass spectrum analysis shows that the compound is huperzine a (HupA), thus a new medicinal herb source for treating alzheimer's disease is provided.

The invention relates to the technical field of microbes and especially relates to huperzia serrata endophytic fungi and its use in production of huperzine a. The huperzia serrata endophytic fungi are named as Colletotrichum sp. SY-1, are preserved in the China center for type culture collection (CCTCC) on August 19, 2011, and have the preservation number CCTCC M2011293. Through fermentation, the huperzia serrata endophytic fungi can produce a huperzine a compound. Through utilization of characteristics of microbe-based huperzine a production and the modern fermentation breeding technology, huperzine a industrial production is realized; bottleneck problems of huperzine a exploitation are solved; and the endangered huperzine a natural resource is protected.

The invention discloses a huperzia serrate endophytic fungus which is obtained from a pteridophyte, namely, a huperzia serrate plant by adopting a separating and purifying technology, is named Ceriporia lacerate HS-ZJUT-C13A and is collected in the China Center for Type Culture Collection on October 28, 2012 with the collection number CCTCC M 2012433. The strain provided by the invention can be used for preparing a compound, namely, 8alpha, 15alpha-epoxydized huperzine A with a nerve protection effect. A conversion method has the advantages of simple fermentation conditions, high substrate conversion rate and the like, is easily cultures the strain, has the potential of industrial large-scale production, is a novel way for obtaining the compound, protects valuable and rare medicinal huperziaceae plant resources from being damaged and can provide a novel thought for reliving the current situation of lack of huperzine A in clinical administration.

The invention discloses an extraction method of huperzine A. The extraction method comprises the following steps: fermentation treatment of leaves of huperzia serrata, diethyl ether soaking, centrifugation, water bath evaporation and cooling crystallization. By virtue of the extraction method, the yield and extraction purity of huperzine A can be greatly increased, and furthermore, the production cost is low; and the extraction method is suitable for industrial production.

A rapid propagation method of a huperzia serrata comprises the steps of: selecting water culture matrix and selecting growth regulator and concentration, and particularly comprises the steps of: (1) material preparation: selecting the harvesting time to be between March and July, shearing the material into terminal buds with the length of 5-8cm, using tap water to clean the material firstly, and then rinsing the material in the water added with detergent, and finally using clean water to wash out the material; (2) water culture matrix selection: taking water as a matrix; and (3) hormone combination and concentration selection: combining indolylacetic acid and naphthyl acetic acid which are applied by the growth regulator, wherein the ratio of the indolylacetic acid to the maphthyl acetic acid is 1:1. The rapid propagation method adopts a water culture method to conduct rapid propagation, greatly improves the index of rapid propagation, provides technical support for realizing industrialized production and is low in cost.

The invention discloses a method for separating and purifying huperzine by a molecular imprinting technology, which includes the following steps: 1 huperzia serrata is smashed and extracted in ultrasonic counter-current mode, obtained extracting solution is decompressed and concentrated, and then active carbon is added in the extracting solution to perform decoloration; 2 the decolorized extracting solution is arranged on a solid phase column, huperzine molecular imprinting polymer serving as a solid phase is installed in the solid phase column, 5% to 10% of ethanol water or methanol water is used for elution, 40% to 50% of ethanol water or methanol water is used for elution till eluent is colorless, an disassembling column enables the solid phase to be taken out from the solid phase column, and 60% to 70% of ethanol water or methanol water is used for extracting adsorbed huperzine monomers in the solid phase to obtain extraction liquid; and 3 the extraction liquid is decompressed, concentrated and recrystallized by using solvent to obtain the huperzine. The method for preparing the huperzine has the advantages of being safe, easy and simple to operate, low in cost, short in production cycle, high in purity and the like.

The invention discloses a cultivation method for producing hupenine A through in vitro induction proliferation of thallus of huperzia serrata. The method comprises the following steps: selecting a wilding huperzia serrata plant which is healthy and has no plant disease or insect pests, and taking a new branch which grows in the current year and is 3 cm in length; producing thallus through induction, proliferating into a piece of thalline tissue block with the diameter of 3 cm, and using the two culture mediums as follows: a thalline differentiation launching culture medium and a thalline mulitiplication culture medium; and dividing and subculturing thalline. The cultivation method and a substrate formula provide essential environmental conditions to in vitro induction and proliferation ofthallus of huperzia serrata in production of hupenine A. When the method provided by the invention is used for in vitro culture of huperzia serrata, a great number of medicinal plant resources of thalline of huperzia serrata can be obtained. Significant economic values can be produced when the method is used for enlarging the scale of extracting produced medicine hupenine A (Hup-A) through in vitro culture of thalline.

The invention discloses a fungus strain with high-efficiency expression of huperzine A. The strain is separated from endophytic fungi of wild huperzia serrata (huperziaceae, huperzia), belongs to the NSH-D strain of mucor racemosus and has genetic stability till the passage to 15th generation in a laboratory; and the amino acid sequence is shown by SEQ ID NO:1. The fungus strain with high-efficiency expression of huperzine A, disclosed by the invention, serves as a biological strain for treating the Alzheimer's disease, the good huperzine A generating high-efficiency expression gene is transferred into other type strains, and more good characters are obtained through gene modification; and moreover, further optimization of the strain can be realized through mutation breeding. The study on the fungus strain is of great significance to the biosynthesis and industrial production of huperzine A, protection of plant resources, meeting of clinical pharmaceutical needs and reduction of the medical expense for treating the Alzheimer's disease.

The invention discloses a preparation method of a Chinese medicine irrigation solution for treating erosive type chronic cervicitis, belonging to the technical field of preparation method of Chinese medicines. At present, generally, antibiotics and sulfanilamide medicines are used for treating erosive type chronic cervicitis, and the disadvantage is large toxic and side effect. The Chinese medicine irrigation solution disclosed herein is prepared by putting 29 kinds of herbs consisting of verbena, Galium aparine, dysosma versipellis, wikstroemia indica, saururus chinensis, radix berberidis, folium isatidis, garden euphorbia herb, Pennywort Herb, Rohdea japonica Roth, Aster ageratoides, radix sophorae tonkinensis, edible tulip, senecio scandens, Huperzia serrata, Japanese stephania root, thymifoious euphorbia herb, meadowrue root and rhizome, purslane, leaf of Chinese coriaria, sodaapple nightshade herb, radix scutellariae, coptidis rhizome, cortex phellodendri, Broomjutre Sida Herb, ovateleaf holly bark, starwort root, Chinese Iris seed and liquorice in water for soaking, then decocting with slow fire, filtering and removing the residues to obtain a liquid medicine. The Chinese medicine irrigation solution disclosed herein has the advantages of simple preparation, small toxic and side effect, short treatment course and high cure rate.

The invention is directed to the field of natural medicine, specifically to alkaloid extracts of Huperzia serrata (Thunb.) Trev. A Huperzia serrata (Thunb.) Trev. composition and the method for preparing it are provided. The Huperzia serrata (Thunb.) Trev. composition comprises Huperzine A and Huperzine B, wherein the total content of the said Huperzine A and Huperzine B is 50-99 wt % of total alkaloids and the weight ratio of the said Huperzine A and Huperzine B is 1:0.5-5. The Huperzia serrata (Thunb.) Trev. composition of the invention has good therapeutic effect and improved safety.

The invention discloses a huperzia serrata hairy root system preparation and cultivation method, and belongs to the biological cell engineering technology. The method comprises the following steps: tender stems of huperzia serrata is adopted for dedifferentiation treatment of an explant to acquire huperzia serrata calluses; the callused and agrobacterium rhizogenes (DL 1968) containing Ri plasmid are co-cultivated and transferred to an induced medium for induced cultivation after residual fungus liquid is extracted by aseptic paper, and then hairy roots grow at the huperzia serrata calluses; and the explant with the hairy roots is placed in an expansion medium for expanded cultivation of the hairy roots after bacteriostatic cultivation. According to the invention, the biological cell engineering technology is utilized to build a huperzia serrata hairy root system cultivation system to prepare a huperzia serrata hairy root system, so that standardized production of huperzia serrata is realized, wild resources are replaced, the ever-increasing market demand for huperzia serrata is relieved, and the problem of dependency on huperzia serrata import is solved. Therefore, the huperzia serrata hairy root system preparation and cultivation method has great significance on industrialized and commercialized development of medicinal plant hairy roots.

The invention discloses a method for producing huperzine A by using huperzia serrata prothallium. The huperzine A is produced from in vitro huperzia serrata prothallium by mass culture. The method is simple and easy to operate, provides new ways for sources of the huperzine A, and protects resource of wild huperzia serrata simultaneously.

Belonging to the technical field of Chinese medicine preparation methods, the invention relates to a preparation method of a Chinese medicinal lotion treating mustard preferring-type cellulitis. Current treatment of mustard preferring-type cellulitis generally employs antibiotics and sulfonamides, and has the disadvantage of great side effect. A technical scheme adopted by the invention consists of: taking sapindus mukurossi, Soda-apple Night-shade Herb, tartarian buckwheat roots and rhizomes, dandelions, Dysosma versipellis, Indian Stringbush Roots, Saururus chinensis, barberry roots, folium isatidis, garden euphorbia herb, Longhairy Antenoron Herb, Rohdea japonica roots, Aster ageratoides, Radix Sophorae Tonkinensis, Asarum sagittarioides, climbing groundsel herb, Huperzia Serrata, Japanese Stephania Roots, Thymifoious Euphorbia Herb, meadowrue roots and rhizomes, portulaca oleracea, Coriaria sinica leaves, Scutellaria baicalensis, Sedum sarmentosum, Sagina subulata, sugarcane, cottonrose hibiscus flowers, Sophora flavescens, bitter gourds, Folium llicis Latifoliae, Elephantopus scaber, polygonum cuspidatum, Saxifraga stolonifera, Polygonum hydropiper and licorice, etc., and putting the 39 medicines into water for immersion, then conducting decocting with slow fire, and carrying out filtration to remove the residue, thus obtaining a liquid medicine, i.e. the Chinese medicinal lotion treating mustard preferring-type cellulitis. The prepared Chinese medicine has the advantages of small toxic and side effect, short treatment course, and high curative rate.

The invention discloses a preparation method of traditional Chinese medicine lotion for treating heat-toxin colpitis mycotica, belonging to the technical field of traditional Chinese medicine preparation methods. At present, the heat-toxin colpitis mycotica is generally treated by antibiotics and sulfonamides, causing great toxic and side effects. The traditional Chinese medicine lotion for treating heat-toxin colpitis mycotica is prepared by the following steps of: soaking fig, Chinese soapberry fruit, soda-apple night-shade herb, Tianqiaomai root, dandelion, dysosma versipellis, root of Indian stringbush, saururus chinensis, radix berberidis, folium isatidis, garden euphorbia herb, longhairy antenoron herb, rohdea japonica root, aster ageratoides, radix sophorae tonkinensis, cremastra appendiculata makino, climbing groundsel herb, huperzia serrata, Japanese stephania root, thymifoious euphorbia herb, meadowrue root and rhizome, herba portulacae, leaf of Chinese coriaria, polygonum hydropiper, sugarcane, hlblsclls llutabilis, radix sophorae flavescentis, bitter gourd, broadleaf holly leaf, elephantopus scaber, rhizoma polygoni cuspidati, saxifraga stolonifera, umbilicaria esculenta, lycoris radiata and liquorice in water; decocting with slow fire; and filtering to remove slag to obtain the liquid medicine. Through the invention, the prepared traditional Chinese medicine has the advantages of little toxic and side effect, short course of treatment and high cure rate.

The invention discloses a fungus strain with an Alzheimer's disease treating function and application of the fungus strain. The fungus strain is separated from wild huperzia serrata endophytic fungi and belongs to the Trichoderma harzianum NSW-V strain, the fungus strain has genetic stability after 15 generation passages in a lab, and the amino sequence of the fungus strain is as shown in SEQ ID NO. 1. The fungus strain with the Alzheimer's disease treating function has the advantages that the fungus strain serving as the biological strain for treating the Alzheimer's disease transfers the excellent efficient expression genes for producing huperzine a into strains of other modes, more excellent properties are obtained through gene modification, and the strain can be further optimized through mutation breeding; the researches of the fungus strain are greatly significant to the biological synthesizing and industrial production of the huperzine a, plant resource protection, satisfying of the clinical front-line medicine use requirements, and lowering of the medical cost for treating the Alzheimer's disease.

The invention discloses a method for separating traditional Chinese medicine huperzia serrata total alkaloids, in particular to a method for separating total alkaloids from traditional Chinese medicine huperzia serrata extract, belonging to the traditional Chinese medicine field. The method includes the following technical scheme: huperzia serrata extract is taken, pH value is adjusted to be 1-7, filtering is carried out, filtrate is taken and added onto cation exchange resin, eluting by water is firstly carried out to remove impurities, and then 0.5-20% saline solution is used for socking the resin, and then 0.5-20% saline solution containing acid or alcohol is used for eluting until no alkaloid is detected, eluant is collected and added with alkali to be natural, and desalinization treatment and concentrated drying are carried out, thus obtaining the traditional Chinese medicine huperzia serrata total alkaloids. The method overcomes the defects of low extraction rate, high organic solvent dosage, excessive high acid-base concentration, complex technology and impossible for large production and the like of the prior art and can efficiently separate high purity huperzia serrata total alkaloids in traditional Chinese medicine huperzia serrata extract.

The invention relates to a composition capable of treating vascular dementia, which is prepared from the following raw materials in parts by weight: 10-20 parts of ginseng, 10-20 parts of ginkgo biloba, 10-20 parts of huperzia serrata, 5-15 parts of szechuan lovage rhizome, 5-15 parts of earthworm and 5-15 parts of polygala root. The composition has simplified composing prescriptions and accurate curative effect on curing vascular dementia. The invention further relates to a preparation method of the composition, which comprises the following steps: taking ginseng, grinding, and extracting ginsenoside; taking ginkgo biloba, and extracting the total flavone of ginkgo biloba; taking szechuan lovage rhizome, and extracting essential oil; and decocting the residual medicinal materials, merging decocted liquid, filtering, concentrating to condensed paste, adding the ginsenoside, the total flavone of ginkgo biloba and the essential oil of szechuan lovage rhizome, adding auxiliary materials, and preparing a pharmaceutically acceptable preparation. The method is adopted to prepare the composition so that the composition can be ensured to be used conveniently and have controllable quality.

The invention relates to a novel method for extracting huperzine A from Huperziaserrataf.longipetiolata (Spring) Ching, and relates to the technical field of medicines. The novel method comprises the technical scheme as follows: (1) preparing extracting liquid; (2) purifying the extracting liquid; (3) preparing the total alkaloid of huperzia serrata; (4) preparing a rough product of huperzine A; and (5) preparing high-purity huperzine A, thus obtaining the white solid huperzine A with the purity of 98% to 99%. The novel method of extracting the huperzine A provided by the invention has the advantages that: (1) the new resource huperzia serrata is served as the raw material; and the single source of the medicine and technology method for extracting huperzine A from huperziaserrataf.longipetiolata (Spring) Ching are expanded; (2) 20% to 60% of ethanol water is adopted for extracting, therefore, little damage is brought to instruments and equipment, and low toxic and side effects are generated; and (3) the huperzine A is enriched through a reverse phase (Middle Pressure Liquid Chromatography) is adopted, thus, the cost is low, the purity is high, the yield is high, and the mass industrial production can be easily carried out.

The invention discloses a test tube cottage method for huperzia serrata sporophyte. The method uses terminal buds of fresh huperzia serrata sporophyte as materials, and reproduction and preservation of the materials are performed in a test tube cottage mode. The method is simple, easy to operate and high in efficiency, has important significance to preservation of the material resources of huperzia serrata and can provide fresh and alive experimental materials for laboratory studies on relevant aspects in various regions.

The invention discloses an effective huperzia serrata callus culture method. The method includes: adopting a detergent to wash a huperzia serrata stem tip twice, adopting sterile water to wash the same for 4 times, adopting 0.15% mercuric chloride liquid to sterilize for 5min, and adopting sterile water to wash for 4-6 times; taking down a micro stem tip of 0.1-0.2mm in length from the huperzia serrata stem tip under an anatomical lens, adopting an MS culture medium, adding 0.1uM of IBA, 1.5uM of KT and 3mg / L glutamine, and performing photoperiod culture at constant temperature of 25 DEG C for 14h. A method of repeated sterilization combined with AAS to inhibit endophyte is adopted, so that the problem of endophytic fungi pollution in the process of huperzia serrata callus culture is solved, and callus induction rate is higher than 25% generally.

The invention discloses a hormone-autotrophic huperzia serrata in-vitro thallus cloning line for producing huperzine-a. A method comprises the steps are of (1.1) preparing materials; (1.2) preparing a germfree material from an explant and; (1.3) inoculating the germfree material to a prepared thallus differentiation starting culture medium for culture; (1.4) partitioning a thallus tissue subjected to inducible proliferation into small blocks, transferring the small blocks into a thallus subculture multiplication culture medium for directional culture; (1.5) subculturing the hormone-autotrophic huperzia serrata in-vitro thallus cloning line for producing the huperzine-a. Thallus of the hormone-autotrophic huperzia serrata in-vitro thallus cloning line is directly harvested for preparing tea or serving as an additive of tea, so that a novel health-protection product for resisting aging and enhancing memory can be developed; the medical component, namely huperzine-a, can be extracted from the thallus, so that extremely high economical and social values can be achieved.

The invention discloses a Fusarium endophytic fungus with huperzine-A-producing function. The strain is separated from a wild Huperzia serrata (Huperziaceae, Huperzia) endophytic fungus, belongs to a (Fusarium verticillioides) NSH-5 strain, and has hereditary stability after 20 generations of passage in the laboratory. The amino sequence is disclosed as SEQ ID NO:1. The fungus strain can be used as a biological strain for treating vascular dementia and senile dementia; the excellent huperzine-producing high-efficiency expression gene is transformed into the type strains to obtain more favorable characters by gene modification; and besides, induced mutation breeding can be performed to further optimize the strain.