422 results about "Shoot apex" patented technology

Multiple shoot structures are induced from plant tissues (e.g., shoot apices or axillary buds on an artificial medium) to produce multiple shoot cultures. These multi-shoot cultures are then transformed by known transformation methods. Plants are subsequently regenerated from the transformed cells. Crops that may be efficiently transformed by this method include plants normally recalcitrant to transformation such as sugar beet, sunflower, soybean, cotton, tobacco, tomato, peanuts, melons, watermelon, squash, Brassica, and pepper.

The invention relates to a high-efficiency breeding method of healthy seedlings of cane, the method is proposed aiming at the problems that seed performance degenerates, yield is greatly reduced and the quality is reduced, which are caused by that the cane is taken as asexually propagated crop, propagation coefficient is low, the amount of seeds used for cane implantation is large, and after the implantation of a plurality of years, the cane is easy to be infected with a disease; improved type healthy seedlings of the cane can be cultured by nine steps of seedling mild water detoxication processing, seedling sprouting, in vitro peeling inoculation, induction culture, virus check, propagation culture, rootage culture, domesticated seedling-refining and outdoor temporary planting; the method adopts the combination of mild water detoxication and stem tip detoxication with thorough detoxication and good effect; the direct peeling inoculation of the explant does not need to be treated with disinfection processing, has low pollution rate and light browning, and is easy to generate clustering buds by induction; the propagation coefficient is high, the speed is high, the period is short; the seed performance is table, the seedling is strong; the virus detection is precise and accurate; the survival rate of the outdoor temporary planting is high and is up to more than 90 percent; the production cost is low, the efficiency is high, and the method is suitable for industrialized production.

The fast tissue culture propagation process for azalea and camellia includes the following steps: cutting tender stem segment from the maternal plant in April or May; soaking in alcohol solution, sterilizing in HgCl2 solution and cutting the stem tip; inoculating the stem tip in primary culture medium to grow cespitose bud and cutting single bud seedling after the bud grows to 2-3cm length; culturing the single bud seedling in secondary culture medium inside a sealed container to proliferate test tube seedling repeatedly; culturing the test tube seedling in rooting culture medium inside sealed container to grow new root to 0.3-0.5cm long, and transplanting. The process can provide seedling of azalea and camellia continuously.

The invention discloses root tip detoxification and rapid propagation technology for purple potatoes and belongs to the technical field of plant detoxification and tissue culture rapid propagation. The method comprises the following steps of: (1) preparing a culture medium; (2) performing root tip detoxification and rapid propagation; and (3) performing multi-reverse transcription-polymerase chain reaction (RT-PCR) synchronous and rapid detection on complex virus infection of a purple potato test tube plantlet or a test tube mini-tuber and the like. In the technology, each level of culture medium is adjusted according to the characteristic of the purple potato and conventional stem tip detoxification is changed into root tip detoxification so as to simplify operation process, avoid high pollution rate and improve working efficiency. Moreover, the technology has the advantages of good inoculant differentiation, high test tube plantlet propagation speed, complete detoxification, high mini-tuber growth vigor, monthly mini-tuber reproduction rate of 4 to 6 times and mini-tuber rooting rate and transplanting survival rate of over 98 percent. The technology can be popularized in regions which are suitable for the growth of purple potatoes.

The invention discloses a dendrobium officinale sprout rapid propagation method with high efficiency. The method comprises the following steps: a) selecting dendrobium officinale shoot tip or aseptic seedling as explants, performing induction and propagation culture of protocorm like bodies; b) performing differentiation and seeding formation on protocorm like bodies; c) culturing strong plantlet and rootage of seedling grown thickly; and d) hardening seedling and transplanting of the aseptic seedling. According to the invention, shoot tip and aseptic seedling are taken as the explants, thereby the problem that stem bud and top bud as the explants to induce the protocorm like bodies, the explant source is limited can be solved, the mature protocorm like bodies can be rapidly and continuously obtained with large amount, the incidence rate of the protocorm like bodies can reach as high as 99%, the seedling differentiation coefficient can reach as high as 50 strains/0.1g protocorm like bodies, the period for acquiring the seedling is 4 months, the seedling is tidy, the rooting rate is 100%, the root length is about 5cm, and the root number can reach 10 strips/crowd, the transplanting survival rate can reach as high as more than 95%, and the purposes of shortening the seedling period, increasing the propagation coefficient and unlimited germplasm source can be reached. The rapid propagation method can rapidly produce large batch of sprouts, and is suitable for large scale production.

The present invention discloses a pipless fructus momordicae and the cultivation method. The cultivation method is: 1) breeding the tissue cultivation seedling with the male and the female ex-plant of diploid fructus momordicae; 2) doubly introducing the chromosome with the tissue-cultivation successive-transfer seedling; 3) performing the differential cultivation with different parts of the male and the female plants after introducing treatment; 4) cutting the stem tip and the stem section of the sprout differentially cultivated to perform the gemmulation cultivation; 5) examining the number of the chromosome of a cultivated entire plant to select the tetraploid plant for performing the root cultivation and the seedling exercise; 6) planting the tetraploid plant and the diploid plant according to the conventional techniques and performing the artificial pollination to get the hybrid seed when in flower; 7) breeding the hybrid seed to be a complete plant and examining the number of the chromosome; selecting the triploid plant to perform the root cultivation and seedling exercise; 8) planting the triploid plant and the diploid plant and performing the artificial pollination on the male diploid plant with the female triploid plant when in flower; the pipless fructus momordicae can be obtained after the fruiting of the female plant.

The invention provides a method for culturing and rapidly propagating the stem tip tissue of a rare cymbidium goeringii. By taking the stem tip of a bud of the rare cymbidium goeringii as an explants, the method comprises the following steps: inducing rhizome from the stem tip, performing successive multiplication on the rhizome, inducing the bud from the rhizome, rooting and strengthening the seedling, transplanting the test-tube plantlet and the like; an optimal culture medium is chosen at the stages of cutting and sterilizing the bud, stripping the stem tip, inducing the rhizome, multiplying, bud differentiation, rooting and strengthening and the like, so that quick multiplication of the seedling, high yield, good quality and low cost of the rare cymbidium goeringii can be realized to lay the foundation for the industrial production of the cymbidium goeringii. The method of the invention has high operability, well solves the problems of slow multiplication speed, easy degeneration and the like in a traditional division method of the cymbidium goeringii, can gradually realize the industrial production of the cymbidium goeringii species of high commodity value and has high application and popularization value.

The invention relates to a tissue culture propagating method of Manihot esculenta Crantz. As one of the global three-top tuber crops and the number one alimentarn crop in Africa, the Manihot esculenta Crantz is currently ranked as a vital biological energy crop in our country. Stem section vegetative cottage is commonly adopted for propagation, and the low propagation rate thereof is a restrictive factor for popularizing the new variety and shortening the breeding period. The method of the invention comprises the following steps: the Manihot esculenta Crantz stem section with stem apexes or lateral buds is taken as an explant, and the Manihot esculenta Crantz tissue culture plantlets are quickly obtained in virtue of the micro-propagation method; young leaves, stem apexes and axillary buds of the sterile plantlets obtained from micro-propagation are taken as explants, somatic embryo generation and plant regeneration are induced, thus the Manihot esculenta Crantz somatic cell regeneration system is established. By adopting the method, the Manihot esculenta Crantz has the advantages of high propagation rate, no genotype dependence, and the like; the method has high value on the quick propagation and large-scale production of the Manihot esculenta Crantz improved variety in a short term, and establishes a technical basis for the Manihot esculenta Crantz transgene and other breeding.

The invention provides a process for producing the oriental lily sterilized seed globule in the bottle, comprising: choosing explant, sterilizing, inducing culture and getting the sterilized stem tip, then breeding culture, subculture, and producing the sterilized seed globule in the bottle directly. The invention produces the sterilized seed globule in the bottle directly to avoid the problem of long period of ball forming and the accumulation of virus existing in the oriental lily, the seed globule can be planted in the soil when out of the bottle, and the survival rate is high. The breeding procedure of the sterilized seed globule is simplified by modifying and optimizing the whole producing procedure for the lily seed globule, and the breeding time of sterilizing the oriental lily is shortened greatly, the quality of seed globule is improved when the producing cost is decreased, which reinforces market competitive power of the domestic self-breeding seed globule and is suitable for the mass production of the oriental lily sterilized seed globule.

The invention provides a method for quickly cultivating Blumea riparia medicinal material, which comprises that collecting Blumea riparia explants in wild, which it contains stem sharp, young stem, old stem, blades, bulbs, and seeds, to be washed and disinfected, and using plant organism quick cultivating method and grafting cultivating method to quickly cultivate the Blumea riparia, cultivating seeds, while the obtained seed can be directly used in field. The invention has high cultivate speed, short period as 2-3 months, with high survival rate more than 90%, simple operation, simple device, low cost, and application in whole year. And the product cultivated by the invention has stable gene character, without variation.

The invention discloses a tissue culture and rapid propagation method for kadsura coccinea. The method includes the step A of selection and processing of explants, the step B of induction of explants, the step C of browning prevention processing of calluses, the step D of differentiation of calluses, the step E of subculture multiplication culture, the step F of strong seedling culture, the step G of rooting culture and the step H of seedling hardening and transplanting, wherein in the step A, young and tender leaves close to tips of kadsura coccinea stems are taken and disinfected to obtain sterile explants. By means of the method, the browning rate of explants of kadsura coccinea is controlled at 7% or below, the rooting rate reaches 95%, and the seedling formation rate reaches 97% or above.

A fast propagation method of tissue culture of Dendrobium primulinum Lindl belongs to the technical field of plant fast propagation. The method is characterized by comprising the steps: 1) a young stem of the Dendrobium primulinum Lindl with a length of 3 to 5cm is chosen as an explant; 2) the treating of the explant; 3) inoculation and culture for 10 to15 days in an aseptic inoculation chamber; 4) a survived stem tip is transferred to a inducing medium for inducing an adventitious bud or a protocorm which, after 30 to 40 days, is transferred to a reproducing medium for reproducing of 2 to 3 months; 5) successive transfer and rooting culture; 6) a single plant is transplanted in a 128 plug-tray. The fast propagation method of tissue culture of the Dendrobium primulinum Lindl is not affected by seasons. Due to the easy controlled propagation environment, the Dendrobium primulinum Lindl adapts to various places of China. Therefore, the problems of hard-breeding, easy degeneration, quick variation and irregular seedling quality of the Dendrobium primulinum Lindl are resolved. Seedlings of the Dendrobium primulinum Lindl with good and uniform quality are produced and have wide market prospect.

The invention provides a method for cryopreservation and plant regeneration of Dianthus caryophyllus shoot tips. Growth-restored regenerated Dianthus caryophyllus plants are obtained through preculture, loading, dehydration, freezing, thawing, restoration culture and the like. By adopting the method provided by the invention, the regeneration rate of Dianthus caryophyllus can reach 52.4 percent, the device for the vitrification cryopreservation of the Dianthus caryophyllus shoot tips is simple, the process is simplified, the operation is easy, the freezing effect is good, the restoration growth of the preserved Dianthus caryophyllus is good, the hereditary variation probability is small and the method is an effective way for the in vitro preservation of Dianthus caryophyllus germ plasm resources.

The invention provides a local breeding technology for the virus-free seedling of Korla pear. The technology comprises the following steps: 1) raising the seedling of Korla pear by grafting; 2) raising the virus-free seedling by using the stem tip of Korla pear; 3) carrying out T-shaped bud grafting; and 4) cultivating Korla pear by using a flat shed net rack. According to the invention, through virus-free cultivation and micro-grafting of the stem tip of Korla pear, virus-free cultivation of the seedling of Korla pear is realized and the high-quality Korla pear seedling is produced; since appropriate rootstocks are selected from local Korla pear rootstocks, a cultivation model for Korla pear applicable in acidic soil is established; and since Korla pear has a shed frame shape, ventilation and light transmission are realized, strong wind resistance and high fruit quality are obtained, and management is easy.

The invention relates to a factory propagation method for detoxified red sweet potato. The method comprises the step of detoxifying test-tube plantlets, breeder seeds, stock seeds and improved variety. The method specifically comprises the following steps: firstly, cleaning potato blocks, using 800-times diluted solution of 50% carbendazim for disinfecting for 20min and then performing 26 DEG C constant temperature sprouting; adopting a high/low temperature alterative treating mode of treating for 16 hours at 26 DEG C and treating for 8 hours at 35-40 DEG C in each day for continuously treating for about 30 days when the buds grow to 0.1-0.5cm; performing stem apex detoxification, rapid tissue culture propagation, rooting and seedling hardening, rapid water culture propagation, potato vine transplanting and breeder seeds propagation; and using the breeder seeds for rowing potato and seedling in the greenhouse with a fly net in the next year; transplanting into field, performing field management, controlling pests, timely harvesting and storing the stock seeds before frosting and propagating the excellent stock seeds. According to the invention, the detoxified seedlings can be subjected to factory propagation within a short period of time, the production cost of the detoxified seedlings can be reduced and the lastly acquired detoxified improved variety is convenient for the peasant to plant, transport and store.

A tissue culture method for clematis Multi-Blue includes such steps as disinfecting the stem tip of clematis multi-blue by alcohol or corrosive sublimate, flushing with aseptic water, primary culture, secondary culture, rooting culture and reproducing embryoids.

The invention discloses an orient lily tissue culture detoxification method, and selects healthy lily and cut into squamas, induces adventitious buds; to transplant the adventitious buds to the growth substrate, after 30 days cultivation, to cut off the stem tip and transplant in the growth substrate for variable temperatures cultivation for 30-60 days again, and then the needed nontoxic plant series is obtained. The invention has obvious detoxification effect, high efficiency which can reach 90-100 percent commonly. With simple operation and low cost, and thereby the invention has bright application prospect.

The invention discloses a large-power mulberry shoot reaper, which comprises a traveling mechanism, a power mechanism and a cutting mechanism, wherein the traveling mechanism is a tricycle or a quadricycle, the power mechanism is a main transmission device which is driven by a diesel engine, the main transmission device is connected with front wheels or rear wheels of the tricycle or the quadricycle, the cutting mechanism comprises a branch collection bracket which is arranged on the front end of a head of the tricycle or the quadricycle and rotary-type cutters corresponding to each guide groove on the branch collection bracket, each guide groove is in a flaring shape with a large front port and a small rear port, each rotary-type cutter is horizontally arranged below the rear port of each guide groove, a cutter transmission device is connected with the main transmission device, and branch supporting rods which rotate coaxially and synchronously with the rotary-type cutters in the same direction are arranged above the branch collection bracket. The large-power mulberry shoot reaper is driven by a large-power cutting power source and a large-power mechanical power to travel, and the rotary-type cutters are adopted, so the working efficiency is effectively improved on the basis that the reaping quality is guaranteed, and the labor intensity is alleviated.

The invention relates to Chinese toon tissue culture and rapid propagation technology. The technology comprises the following steps: clipping an annual semi-lignified branch with plump axillary buds from a growing plant, clipping the branch into a stem section containing 1 to 2 axillary buds after sterilization, and rapidly inoculating the stem section to a differentiation induction culture medium for differentiation culture; placing the plant obtained through the differentiation culture into a propagation culture medium for propagation and rapid propagation; and when a certain quantity is reached through the propagation, putting the plant into a rootage culture medium for sound seedling rootage culture, and hardening the seedling after the sound seedling takes root. The method carries out the asexual tissue rapid propagation and disinfection through the stem apex and stem section of Chinese toon. The propagated filial generation maintains the good seed nature of the original variety, ensures the seed purity and has no diseases and insects. The technology overcomes the defect of low propagation speed lying in the conventional seed and cut root propagation, enables the artificial control on the culture and growth conditions, is not limited by natural conditions, and has a small amount of drawn material. The technology can realize the industrialized seedling culture and high-efficiency production.

The invention relates to the propagation production of cane seedlings, and provides a method for removing cane perennial root dwarfing germs and rapidly propagating healthy cane seedlings, which comprises the following steps: little amount of original seeds are treated by hot water, stem tips which are detected to be feminine by the regenerated plantlet by carrying out speckle enzyme marker immune test (DB-EIA) is taken as an explant, healthy seedlings are produced by induction culture, secondary propagation culture, root-promoting culture, temporary planting and second-stage or third-stage nursery propagation-extension; furthermore, the disinfection of seed-cutting tools (cane knives) is paid attention to in the nursery propagation-extension, before the seedlings are used as seeds for large field production after coming out from nursery, cane stem juice is extracted by sampling, and RSD virus is detected by using the DB-EIA, thus guaranteeing the providing of qualified seedlings. The method has the advantages that: dwarfing disease pathogenic bacteria of the perennial root of the cane seedling can be thoroughly removed, annually propagation coefficient can reach as high as more than 10 thousand times, furthermore, the seedling has no variation, has the advantages of being fast, high-efficient and safe, and is suitable for the industrialized and standardized production of the cane healthy seedlings on scale.

The invention discloses a method for establishing a lotus regeneration system. The method comprises the following steps: breaking shells of sterilized Guangchang white lotus seeds by using pruning shears; taking out mature lotus nuts (embryos) as explants to culture aseptic seedlings; cutting 2-3mm of stem tips and folded tender leaves (with short petiole) as explants for inducing callus from the aseptic seedlings after 2 months; inducing and differentiating the callus to develop buds after 2 months; after 1 month, cutting off the black callus at the bottom, and proliferating the differentiated seedlings; after 3 months, inducing the differentiated seedlings to develop roots; and strengthening, domesticating and transplanting the rooted tissue culture seedlings after 1 month. By adopting the method, technical basis is provided for transgenic technology of lotus in China, and the inductive callus rate of lotus is increased and the callus is large and stable in size compared with a conventional technique; the propagation coefficients of adventitious buds are increased; as tender leaves of aseptic seedlings of lotus are innovatively taken as explants for inductive callus and are successfully differentiated to obtain seedlings, the material selection of lotus inductive callus is widened, and material guarantee is provided.

The invention relates to a stem tip detoxification and cultivation method for a sweet potato virus seedling. The method comprises the following steps: (1) taking a sweet potato block infected by sweet potato feathery mottle virus and/or sweet potato G virus; cultivating the sweet potato block indoors to sprout; (2) shearing scissoring terminal buds growing to 1.5-2.0 cm for disinfection; (3) peeling off the stem tips with two leaf primordiums in a sterile manner among the terminal buds as explants for vaccination cultivation to obtain regeneration plants; (4) conducting 2-3 times of successive transfer cultivation on the regeneration plants obtained in the step (3) to build a strain; conducting inspection by adopting an indicator plant method and a cellulose nitrate membrane ELISA in sequence to obtain a detoxified strain; (5) proliferating the detoxified strain quickly to obtain the detoxification seedling. The method can quickly build cultivation system suitable for stem tip cultivation and rapid propagation detoxification seedlings of majority of sweet potato varieties/strains.