204 results about "High cell" patented technology

The present disclosure provides an interdigitated back contact cell and a manufacturing method thereof. The interdigitated back contact cell includes: a substrate including a front side and a back side, the front side being arranged opposite to the back side; where, along a first direction, first functional regions and second functional regions are alternately arranged on the back side of the substrate; an isolation region is arranged between every adjacent first functional region and second functional region; and the first emitter is spatially isolated from one adjacent second emitter by a corresponding isolation region; and the first diffusion layer is in contact with at least one adjacent second diffusion layer in a corresponding region of a corresponding isolation region. The interdigitated back contact cell provided by the present disclosure has a lower reverse breakdown voltage, a high component reliability, and a high cell efficiency.

The invention provides a coordination nano assembly of chiral cyclopeptide and manganese ions as well as a preparation method and application of the coordination nano assembly. According to the method, a manganese superoxide dismutase protein structural domain and a unique tumor microenvironment of melanoma high tyrosinase are combined, and polypeptide sequences L-YD-hL-DD-h, L-YL-HL-DL-H and D-yD-hD-dD-h with different chirality are designed for the first time; the chirality of the polypeptide is regulated and controlled, the in-vivo circulation stability and the cell entry efficiency of the nano assembly are improved, the coordination self-assembly of manganese and different chiral cyclopeptides is realized, and the in-situ oxidation of an in-vivo tumor microenvironment is simulated for photo-thermal therapy and cell membrane coating. The method disclosed by the invention is simple, mild in experimental condition and easy to operate, the prepared cell membrane coated nano assembly not only has long circulation stability and high cell entry efficiency, but also can realize mild photo-thermal therapy through in-situ oxidation, releases manganese ions to activate a CGAS-STING pathway for immunotherapy of tumors, and has a wide application prospect. The potential application value is realized in the field of combined treatment of tumors.

The invention belongs to the technical field of back contact cells, and particularly relates to a back contact cell with a specific front surface structure and a cell assembly thereof, and the specific front surface structure comprises a tunneling oxide layer and a specific doped amorphous silicon layer which are sequentially arranged on the front surface of a silicon substrate, a low-doped amorphous silicon layer, a high-doped amorphous silicon layer and an oxygen-doped amorphous silicon layer are sequentially arranged in the specific doped amorphous silicon layer along the direction far away from the tunneling oxide layer, and the specific doped amorphous silicon layer is doped with a P element and contains an H element; the P doping concentration C1 of the low-doping amorphous silicon layer and the P doping concentration C3 of the oxygen-doping amorphous silicon layer are respectively smaller than the P doping concentration C2 of the high-doping amorphous silicon layer, and the specific front structure enables the back contact cell to absorb incident light within 400 nm, and the highest absorptivity exceeds 85%. The back contact battery disclosed by the invention can still keep good performance after being exposed to ultraviolet rays for a long time, the UV reliability of the battery is improved, meanwhile, high light transmittance is realized, and relatively high battery efficiency is also realized.

The invention belongs to the technical field of back contact cells, and particularly relates to a back contact cell capable of reducing the thickness difference between an N-type region and a P-type region and preparation and application of the back contact cell capable of reducing the thickness difference between the N-type region and the P-type region. Wherein the surface of one side, far away from the crystalline silicon substrate, of the amorphous silicon layer is provided with an N-type region and a P-type region which are alternately formed, the N-type region comprises an N-type doped base layer and an N-type transition layer, and the P-type region comprises a P-type doped base layer and a P-type transition layer; wherein the content ratio of phosphorus to silicon in the N-type doped base layer is smaller than 1, the content ratio of phosphorus to silicon in the N-type transition layer is larger than 1, the content ratio of boron to silicon in the P-type doped base layer is smaller than 1, and the content ratio of boron to silicon in the P-type transition layer is larger than 1. The vertical contact resistance can be obviously reduced while the thickness difference between the N-type region and the P-type region is reduced, the high cell conversion efficiency is obtained, the stability of the cell is improved, and the service life of the cell is prolonged; meanwhile, the risk of film explosion in the subsequent process is reduced, and the edge compounding problem is relieved.

The present invention relates to the field of biotechnology, and particularly to a culture medium, a composition and a culture method for human NK cells. Through optimization, the present invention provides a culture medium, a composition and a culture method for NK cell culture. The culture medium, the composition and the culture method are used for NK cell culture, with clear culture components, simple culture steps, low cost, good amplification multiple of the cultured NK cells and high cell purity. At the same time, the cultured NK cells have good tumor cell killing ability and have good market promotion value.

To provide a shellac modified object which can be changed from a state where cell adhesiveness to a mammalian cell is high, to a state where the cell adhesiveness is low.SOLUTION: A shellac modified object is expressed by a formula (1). In the formula, Z is a part excluding a carboxy group modified by a functional group Y in the structure of the shellac, X1 to X4 are a dialkylamino group, alkylamino group, hydroxy group, halogen group, H or the like.SELECTED DRAWING: Figure 1

The invention belongs to the technical field of cell cryopreservation, and particularly relates to a cell cryopreservation solution and a cell cryopreservation method. The cell freezing medium comprises the following components in percentage by volume: 95% of fetal calf serum and 5% of dimethyl sulfoxide. The temperature conditions for cryopreserving the cells by adopting the cell cryopreservation liquid are as follows: standing for 90 minutes at 4 DEG C, then standing for more than 12 hours or overnight at-70 DEG C, and then immersing into liquid nitrogen for long-term storage. According to the cell cryopreservation liquid and the cell cryopreservation method provided by the invention, the problems that a traditional cell cryopreservation technology and an improved cell cryopreservation box are tedious in operation and low in cell resuscitation success rate, and the improved cell cryopreservation liquid is relatively high in cost are solved, and the cell cryopreservation liquid is simple in ingredient, simple in operation, low in cost and high in cell survival rate.

The invention discloses a plant cell nucleus extraction method and application, and belongs to the technical field of plant single cell sequencing. The method comprises the following steps: carrying out tissue immobilization on plant tissues by using precooled methanol; preparing a cell nucleus lysis solution, namely an NIB solution; cleaning and chopping the fixed tissue sample, and incubating the tissue sample in an NIB solution; filtering with a screen; centrifuging, discarding supernatant, and resuspending and precipitating the NIB solution to obtain a suspension; slowly dripping the suspension into a Percoll solution, and carrying out density gradient centrifugation to purify cell nucleuses. The invention provides a high-applicability and high-quality cell nucleus extraction method which is suitable for cryopreserved or fresh tissue samples, higher cell nucleus yield and mRNA capture rate are obtained, and the data quality of single cell sequencing is improved.

The invention relates to the technical field of biological materials, and discloses a polymer nanofiber as well as a preparation method and application thereof. The polymer nanofiber is prepared from the following raw materials: gelatin, a cross-linking agent, a nano reinforcing phase and a solvent, and the nano reinforced phase comprises nano hydroxyapatite. The polymer nanofiber provided by the invention takes gelatin as a main raw material and takes a substance comprising nano-hydroxyapatite as a nano reinforced phase, and the obtained polymer nanofiber has the advantages of good biocompatibility, high cell activity, large specific surface area, pore structure, good structural stability and low cost, and has the effects of enhancing the affinity between adjacent cells and improving the biocompatibility of the cells. The connection between the cells is enhanced, so that the cell sphere forming capability of the cells is promoted; according to the preparation method of the cell sphere, the polymer nanofiber reinforced cell sphere can be obtained by co-culturing the polymer nanofiber and the cell, the operation is simple, the cost is low, the control is easy, the method is suitable for large-scale culture, the cell sphere forming speed is high, and the structural stability is good.

The embodiment of the invention provides a battery consistency determination method and related products, and the method comprises the steps: determining a voltage difference value corresponding to each frame of data, the voltage difference value being a difference value between the highest cell voltage and the lowest cell voltage in each frame of data; based on the corresponding relation between the voltage difference value corresponding to each frame of data and the battery SOC value in each frame of data, a battery SOC interval is determined, and the voltage difference value corresponding to the battery SOC interval is larger than a set threshold value; and determining the difference value between the upper limit value of the battery SOC interval and the lower limit value of the battery SOC interval as the SOC deviation of the battery, wherein the SOC deviation of the battery is used for measuring the consistency of the battery. According to the embodiment of the invention, the calculation amount for calculating the SOC deviation can be reduced.

The invention discloses a multi-enzyme preparation for industrial cell dissociation as well as a use method and application thereof, and belongs to the technical field of extraction of seed cells for industrial culture of meat. According to the invention, a multi-enzyme preparation composed of a component 1 and a component 2 is adopted; the component 1 comprises neutral protease, hyaluronidase and elastase; and the component 2 comprises matrix metalloproteinase, papain and plasmin. The invention also discloses a preparation method and application of the multienzyme preparation. The reasonable enzyme ratio and the optimized treatment conditions are beneficial to protecting the integrity of cell membranes, maintaining high cell activity and remarkably improving the dissociation efficiency and the cell recovery amount; the complex enzyme preparation is high in adaptability, can be used for various tissue types and dissociation requirements, and is convenient for standardized and large-scale production; in combination with automatic dissociation equipment, the compound enzyme preparation can realize efficient and stable industrial operation, and provides important technical support and wide application prospects for the fields of meat culture industry, tissue engineering, cell therapy and the like.

The embodiment of the specification provides a preparation method of amination modified magnetic beads, and the preparation method comprises the following steps: mixing ferric salt, a ferrous salt aqueous solution and a dextran aqueous solution for reaction to obtain dextran magnetic beads; then adding an epichlorohydrin solution into the glucan magnetic beads to react for a certain time, and then adding an amination reagent to react for a certain time to obtain amination modified magnetic beads; the embodiment of the invention also provides a magnetic bead for cell sorting and cell activation, and a target cell sorting and target cell activation method. The cell sorting magnetic bead provided by the invention is high in cell sorting purity, high in sorting recovery rate and high in cell viability; the activation magnetic bead provided by the invention has a good activation effect, and activated cells can be amplified at high times.

Provided are methods for the creation of ultra-high density cryopreserved cell banks. In certain embodiments, these methods employ altered perfusion culture techniques that allow for production of ultra-high density cell cultures that can be cryopreserved at unexpectedly high cell densities without the need for any cell concentration steps, while retaining excellent cell viability and quality.

The invention relates to a cell-free catalysis system construction optimization method and application thereof. The system comprises efflux pump protein for discharging signal molecules, synthase for synthesizing quorum sensing signal molecules, a transcription factor (TF), a signal molecule response type promoter, lyase and other functional proteins. Wherein the synthetase is used for synthesizing signal molecules; the efflux pump protein is used for discharging signal molecules; signal molecules which are not discharged are responded by transcription factors, generated receptor proteins are combined with the signal molecules and then are induced by the signal molecule response type promoter, the receptor proteins act on the signal molecule response type promoter, and expression of related functional proteins such as lyase is started and regulated. Expression of efflux pump protein is activated by overexpression of efflux pump protein or introduction of activation regulation protein in a cell-free catalysis system, and programmed cell autolysis under high cell density is realized without physical or chemical assistance.

The present invention belongs to the field of cell culture and preservation, and specifically discloses a serum-free and DMSO-free mesenchymal stem cell cryopreservation solution, its preparation method and application. The cryopreservation solution contains the following components: a basic diluent 40-80 v / v%, a permeable cryoprotectant 5-20 v / v%, a non-permeable cryoprotectant 1-10 v / v%, a cell membrane protectant 5-20 v / v%, a cell activity additive 5-20 v / v%, and a plant-derived natural antioxidant composition; the basic diluent contains the following components: sodium chloride, potassium chloride, potassium dihydrogen phosphate, disodium hydrogen phosphate, sodium bicarbonate, D-glucose, HEPES, and sodium pyruvate; the plant-derived natural antioxidant composition is cyanidin-3-O-glucoside C3G and epigallocatechin gallate EGCG; this cryopreservation solution does not contain serum or DMSO, and creatively combines the two plant-derived natural antioxidants C3G and EGCG. The sources and dosages of all components are clear, it does not contain any cytotoxic components, is easy to use, has a high cell recovery viability after cryopreservation, and has a wide range of applications.

The invention discloses a hydrophobic polymer, a preparation method thereof and a single cell encapsulation method based on interaction of a subject and an object. The hydrophobic polymer disclosed by the invention can be embedded into a cell membrane to carry out hydrophobic modification on cells, and anchoring groups are modified on the surfaces of the cells, so that single-cell encapsulation of the cells is facilitated. The single cell encapsulation method based on interaction of the subject and the object comprises the following steps: 1) preparing hydrophobic polymer intercalation modified cells; and 2) carrying out layer-by-layer packaging on the hydrophobic polymer intercalation modified cells by using the cyclodextrin grafted hyaluronic acid and the adamantane grafted hyaluronic acid. The single cell encapsulation method based on host-guest interaction has the advantages of high encapsulation efficiency, high cell survival rate, good biocompatibility, simplicity in operation, mild reaction process, high reaction speed and the like, is suitable for large-scale industrial application, and cells obtained by encapsulation can be used for tissue repair in multiple aspects and are wide in application range.

To provide an aqueous polymer solution for the cryopreservation of a biological sample and a solidified body containing a biological sample, with high cell survival rate.SOLUTION: Provided are an aqueous polymer solution containing a water-soluble polymer or a salt thereof in an aqueous solvent and used for the cryopreservation of a biological sample, the intrinsic viscosity (η) of the aqueous polymer solution being 0.20 dL / g or more and 0.95 dL / g or less, the orthogonal projected area of the biological sample when solidified at -80°C having decreased to less than 8 / 10 compared to before solidification, and in the differential scanning calorimeter (DSC) curve of a temperature cooling process using a DSC, the calorific value of an exothermic peak during the temperature cooling process being 0 J / g, or 65% or less of the corresponding calorific value of the standard solution consisting of water; and a frozen solidified body thereof.SELECTED DRAWING: None

This invention discloses a multi-enzyme preparation for industrial cell dissociation, its usage method, and applications, belonging to the field of seed cell extraction technology for industrial meat culture. The invention employs a multi-enzyme preparation composed of component 1 and component 2; component 1 includes neutral protease, hyaluronidase, and elastase; component 2 includes matrix metalloproteinase, papain, and plasmin. The invention also discloses the preparation method and applications of the multi-enzyme preparation. The rational enzyme ratio and optimized treatment conditions of this invention help protect cell membrane integrity, maintain high cell activity, and significantly improve dissociation efficiency and cell recovery. The compound enzyme preparation is highly adaptable, applicable to various tissue types and dissociation requirements, and facilitates standardized and large-scale production. Combined with automated dissociation equipment, the compound enzyme preparation enables efficient and stable industrial operation, providing important technical support and broad application prospects for the cultured meat industry, tissue engineering, and cell therapy.

The invention discloses a method for low-density amplification of T cells and an obtained product, and belongs to the technical field of cell culture. The method comprises the following steps: activating a cell population containing T cells, and co-culturing with a lentiviral vector carrying a target gene for transduction; the method comprises the following steps: transduction is carried out on cells, then the transduction cells are inoculated into a shake flask at an inoculation density of not higher than 2 * 10 < 5 > cells / mL, shake culture is carried out to realize amplification, and amplification of 30-120 times can be realized. According to the method, a low-density cell microenvironment is optimized through dynamic oscillation, the problems of signal insufficiency, factor dilution and metabolism imbalance are solved, the efficiency bottleneck of traditional low-density amplification is broken through, and the method is suitable for large-scale production. The method has the beneficial effects of simplicity in operation, high cell product viability, high purity, low cost, wide scale adaptability and the like. The invention also provides a T cell product prepared by the method.

To provide solidified bodies containing a biological sample with high cell viability, and to provide production method thereof.SOLUTION: Provided is a solidified body containing a matrix and biological sample containing an aqueous solvent and a water-soluble polymer or salt thereof, the biological sample having an orthogonal projection area reduced by less than 1 and 1 / 3.5 or more compared to that in the culture medium before solidification, the difference between the brightness in the Munsell color system of the region occupied by the biological sample and the brightness in the Munsell color system of the region occupied by the matrix being 3 or less, when viewed from the light emitting side when visible light is transmitted, and no endothermic peak on the DSC curve being confirmed, or the temperature at which the endothermic peak is confirmed exceeding -1.4°C and is 1.1°C or less.SELECTED DRAWING: None

The invention provides a composite insulating layer of a light superconducting cable and a preparation method and application of the composite insulating layer. The composite insulating layer is prepared from the following raw materials in parts by weight: 40-60 parts of fusible polytetrafluoroethylene, 30-50 parts of fluorinated ethylene propylene, 0.5-5 parts of an auxiliary agent, 1-5 parts of filler and 0.1-0.6 part of a nucleating agent. The composite insulating layer of the light superconducting cable has relatively high tensile strength, relatively low dielectric constant and dielectric loss factor, good mechanical property and high insulating strength; compared with the prior art, the composite insulation layer has the advantages of low average cell diameter and high cell density, which indicates that a uniform and fine cell structure is formed in the composite insulation layer, the overall density of the material can be effectively reduced, the insulation layer is light, and the composite insulation layer can be applied to preparation of light superconducting cables for medical equipment and realizes integrity and low loss of high-frequency signal transmission.

The invention discloses a solar cell, a manufacturing method thereof and a photovoltaic module, and relates to the field of photovoltaic technology. The solar cell comprises a silicon substrate, a first passivation layer and an ultraviolet cut-off layer, the first passivation layer and the ultraviolet cut-off layer are arranged on the front face of the silicon substrate in a stacked mode, the ultraviolet cut-off layer is arranged on the side, away from the silicon substrate, of the first passivation layer, the ultraviolet cut-off layer comprises m sub-layers, and the refractive index and the thickness of each sub-layer are controlled, the principle that phase difference of incident light and reflected light on the two sides of the film is used for destructive interference is utilized, antireflection of light with the target wavelength is achieved, and the transmittance of the ultraviolet cut-off layer to light with the medium-long-section target wavelength is improved. Therefore, the problem that the short-circuit current density of the battery is reduced due to the fact that the ultraviolet cut-off layer absorbs ultraviolet light is solved. Therefore, the solar cell provided by the embodiment of the invention has relatively high cell efficiency while being capable of effectively resisting ultraviolet induced degradation.

The present invention relates to the technical field of biomaterials, and discloses a polymer nanofiber, a preparation method thereof and an application thereof. The polymer nanofiber comprises the following raw materials for preparation: gelatin, a crosslinking agent, a nano reinforcing phase and a solvent; the nano reinforcing phase comprises nano-hydroxyapatite. The polymer nanofiber provided by the present invention uses gelatin as the main raw material and a substance including nano-hydroxyapatite as the nano reinforcing phase. The obtained polymer nanofiber has good biocompatibility, high cell activity, a large specific surface area, a pore structure, good structural stability and low cost, and has the ability to enhance the affinity between adjacent cells, strengthen the connection between cells, and further promote the formation of cell spheres by cells; the preparation method of the cell spheres provided by the present invention co-cultures the polymer nanofiber with cells, and then polymer nanofiber-reinforced cell spheres can be obtained. The operation is simple, the cost is low, it is easy to control, it is suitable for large-scale culture, and the cell spheres have a fast sphere formation speed and good structural stability.

The invention discloses an intracellular biomass detection method with small damage to cells and low disturbance, and relates to the technical field of cell detection. The invention provides the intracellular biomass detection method with small damage to cells and low disturbance, which depends on the integrated intracellular biomass detection device and adopts an electroporation sampling mode to carry out molecular sampling, so that the activity damage to the cells is avoided; electroporation sampling and nanopore detection are integrated, a one-way main flow channel of a Tesla valve structure is introduced in a matched mode so as to achieve physical communication and eliminate reverse flow on the fluid side, transfer is completed through a one-way path, the high cell viability is kept, and extracted biomolecules do not need to be manually transferred.

The application provides a kind of all-inorganic CsPbBr3 perovskite solar cell based on NH4Cl additive regulation and application thereof, SnO2 quantum dot solution is spin-coated on conductive film substrate to prepare electron transport layer, after preheating, spin-coat PbBr2 solution containing NH4Cl, annealing forms PbBr2 porous film, then spin-coat CsBr solution again to prepare CsPbBr3 perovskite film, scrape carbon electrode on the surface of CsPbBr3 film to prepare solar cell.The application adds NH4Cl in PbBr2 solution, and the porosity of PbBr2 film is regulated by the gas generated by the decomposition of ammonium chloride during annealing, so that the reaction is more complete, and it is beneficial to the growth of perovskite large grains.At the same time, chloride ions can passivate halogen vacancy defects.The solar cell of the application has good stability and high cell efficiency, and has important practical value and economic value for promoting the industrialization process of perovskite solar cell.

The present disclosure provides an interdigitated back contact cell and a manufacturing method thereof. The interdigitated back contact cell includes: a substrate (101) including a front side and a back side, the front side being arranged opposite to the back side; where, along a first direction (D1), first functional regions (116) and second functional regions (117) are alternately arranged on the back side of the substrate (101); an isolation region is arranged between every adjacent first functional region (116) and second functional region (117); and the first emitter (108) is spatially isolated from one adjacent second emitter (109) by a corresponding isolation region (118); and the first diffusion layer (110) is in contact with at least one adjacent second diffusion layer (111) in a corresponding region of a corresponding isolation region (118). The interdigitated back contact cell provided by the present disclosure has a lower reverse breakdown voltage, a high component reliability, and a high cell efficiency.

A cell group division method based on overburden remodeling coordination belongs to the field of cell group division in single-cell RNA sequencing data analysis, and comprises the following steps: firstly, constructing cell multi-fractal dimension characteristics for single-cell RNA sequencing data so as to better utilize different fractal dimension characteristic information; and then multi-fractal-dimension low-dimensional representation is extracted. Secondly, extracting multi-fractal-dimension fusion representation, multi-fractal-dimension remodeling representation and cross-fractal-dimension prediction representation by adopting an obstacle covering mechanism so as to improve the robustness of fractal-dimension remodeling; and optimizing the initial division loss to obtain the initial division of the cells. Then, a confidence weight is obtained based on the remodeling error, the division entropy, and the suspension to reduce the negative effects of the low-quality fractal dimension data. And the consistency and coordination of cell group division are ensured by minimizing the division loss target, and a final cell group division result is obtained. Compared with other methods, the cell group division accuracy is higher, and the performance is more stable.

The present invention relates to the technical field of state estimation of lithium batteries, and in particular to a state estimation and self-correction method and device for a lithium iron phosphate battery pack. The method comprises: performing real-time SOC estimation on a lithium iron phosphate battery pack; determining whether the average temperature T of the battery pack is abnormal; during charging, measuring in real time dU / dt of a battery cell having the highest cell voltage, if dU / dt is greater than or equal to i / 10, correcting an SOCR to 98%, and calculating a correction value e; during discharging, measuring in real time dU / dt of a battery cell having the lowest cell voltage; if dU / dt is less than or equal to i / 10, correcting an SOCR to 5%, and calculating the correction value e; correcting an SOH on the basis of the correction value e; and calculating an SOE of the battery pack on the basis of the SOCR. In the method, a correction point is added on the basis of the characteristic that a voltage suddenly changes in the first and final stages of charging and discharging, the cumulative error in an ampere-hour integral method is reduced, and an SOC, an SOH, and an SOE are corrected.

This invention provides a functional slurry and its preparation method, a coated separator, and a lithium-ion battery, belonging to the field of lithium-ion battery technology. The functional slurry prepared by this invention has a low film-forming temperature, stable colloidal particles, no agglomeration, stable coating adhesion, and good uniformity in peel strength. When coated on a separator substrate, it can impart heat-resistant safety performance or electrode adhesion characteristics to the separator: low thermal shrinkage rate at high temperatures, excellent electrode adhesion, high peel strength, and no coating powder shedding during processing. The functional coating prepared using this slurry is firmly bonded to the substrate surface, effectively improving the problem of large fluctuations in coating performance, especially peel strength, under different seasons, temperatures, and geographical conditions. This improves the consistency of coated separator products, improves the consistency of cell performance, increases the yield of separators and cells, and reduces the problem of high cell short-circuit rates caused by coating powder shedding.

A semiconductor device includes: an upper conductive line; and a first lower lead, a second lower lead, and a third lower lead arranged in the first direction. The semiconductor device further includes a lower active contact connected to one of the first lower conductive line, the second lower conductive line, and the third lower conductive line. Further, the semiconductor device includes a first high cell, a second high cell, a first small cell, and a second small cell disposed between the upper conductive line and the first lower conductive line, the second lower conductive line, and the third lower conductive line, and arranged in the first direction. Each of the first high cell and the second high cell includes a high pattern and a high source / drain pattern connected to the high pattern. Each of the first small cell and the second small cell includes a small pattern and a small source / drain pattern connected to the small pattern.