57 results about "Morphogenesis" patented technology

The invention discloses a culture system, culture method and application of gastrointestinal tumor chemotherapy drug-resistant organoid, the culture system comprises: a basic culture medium, which is a DMEM / F12 culture medium, and is additionally added with a special growth promoting factor obtained from a natural extract; the multiple growth factors comprise an epidermal growth factor (EGF), a fibroblast growth factor (FGF), a bone morphogenetic protein (BMP) and a vascular endothelial growth factor receptor inhibitor (VEGFRi), and the concentration of each growth factor is accurately screened and optimized; the extracellular matrix is Matrigel modified by physical and chemical methods; the specific additive comprises an antioxidant N-acetylcysteine (NAC). According to the culture system, the culture method and the application of the gastrointestinal tumor chemotherapy drug-resistant organoid, the culture system which is closer to a real environment in vivo is constructed by optimizing basic culture medium components, setting various growth factors and specific additive concentrations and adopting modified Matrigel as an extracellular matrix.

Combinations of morphoregulator polypeptides that can be used to improve the capacity of the plant cells to regenerate embryogenic plant tissues, plant organs, and whole plants are disclosed. Also disclosed are plant cells comprising the polypeptides and related methods for improving the capacity of the plant cells to regenerate embryogenic plant tissues, plant organs, and whole plants.

Disclosed herein are methods for treating a disease or disorder of the central or peripheral nervous system by administering to a subject in thereof an agent capable of modulating the activity or expression of bone morphogenetic protein and activin membrane-bound inhibitor (BAMBI). Additionally, methods for screening agents capable of modulating the activity or expression of BAMBI are disclosed.

Plant cell fate and development is altered by treating cells with cellular reprogramming factors. Embryogenesis inducing embryogenesis factor genes and / or morphogenic developmental genes are used as cellular reprogramming factors, specifically comprising polypeptides or polynucleotides encoding gene products for generating doubled haploids or haploid plants from gametes. Maize microspores treated by contacting the isolated cells with an exogenous purified, recombinant embryogenesis inducing embryogenesis factor gene products and / or morphogenic developmental gene polypeptide results in embryogenesis. The gametes of a maize plant develop into embryoids when transformed with a genetic construct including regulatory elements and structural genes capable of acting in a cascading fashion to alter cellular fate of plant cells. Embryogenesis factor proteins and / or developmental morphogenic proteins expressed from a genetic construct are used for ex situ treatment methods and for in planta cellular reprogramming.

To provide a method for producing a sheet-like cell culture showing a three-layer structure including a cell layer of superficial cells, a cell layer of intermediate cells, and a cell layer of basal cells, in this order. Provided is a method for producing a sheet-like cell culture that includes culturing a progenitor cell population in a second compartment separated from a first compartment via a porous member to form a sheet-like cell culture in the second compartment. The sheet-like cell culture includes a cell layer of superficial cells derived from the progenitor cell population, a cell layer of intermediate cells derived from the progenitor cell population, and a cell layer of basal cells derived from the progenitor cell population, in this order. The first compartment holds an induction medium containing retinoic acid, bone morphogenetic protein (BMP), and fibroblast growth factor (FGF).

The invention discloses a construction method and application of a cerebellar organoid model, and belongs to the technical field of organoid culture. According to the method, the stability and repeatability of cerebellar organs are remarkably improved by optimizing the components of the culture medium and the induction process. Key morphogenetic factors are regulated and controlled in a stepped mode, and cells are accurately guided to be directionally differentiated to cerebellum; meanwhile, a culture medium without Matrigel is used in the nerve expansion and maturation stages, batch variation of exogenous matrixes is eliminated, and development path consistency and cell composition uniformity are ensured. The organoid constructed on the basis of the method has remarkable advantages in SCA3 disease modeling, single cell sequencing proves that the difference in batches is remarkably smaller than that of an existing method, and a reliable in-vitro human model platform is provided for SCA3 mechanism research and drug screening.

The application provides a bone morphogenetic protein BMP-2 recombinant protein, and the amino acid sequence of the BMP-2 recombinant protein is shown as SEQ ID No. 1. The coding gene, the expression plasmid, the prokaryotic expression vector and the prokaryotic expression method are also disclosed. The prokaryotic expression system is adopted, and a solubilization tag is creatively introduced, so that the inclusion body expression is converted into soluble expression. The BMP-2 is expressed in the form of inclusion body in the prokaryotic system, the protein is converted into soluble expression by adding the solubilization tag, the difficulty of protein separation and purification is reduced, the purification cycle is shortened, and the purification efficiency is improved.

The invention provides a bone repair material and a preparation method and application thereof, the bone repair material is composed of BMP protein (bone morphogenetic protein), carboxymethyl chitosan and calcium chloride, the carboxymethyl chitosan is crosslinked through calcium chloride to form nanoparticles, and the BMP protein is effectively loaded through electrostatic interaction; the bone repair material has good biocompatibility, biosafety and biodegradability, not only can effectively wrap and protect BMP protein from rapid degradation by enzyme, enhance in-vivo stability of BMP protein and realize continuous and controllable release of BMP protein, but also is beneficial to reduction of oedema and ectopic bone risks due to low-dose medication, and has good application prospects. And finally, by promoting angiogenesis, promoting bone tissue development and relieving inflammation, formation and repair of bones are achieved.

The application belongs to the technical field of biology, and particularly relates to a dsRNA of a target Helicoverpa armigera HaPburs gene and application thereof in prevention and treatment of the Helicoverpa armigera. HaPburs RNA interference mediated knockdown reduces the transcript level by 60.01%, and induces morphological abnormalities of pheromone glands (PG), which are irregular black protrusions; the silencing HaPburs reduces the main pheromone component Z 11-16: Ald content, while the male insect attraction rate and mating rate, cumulative egg production also significantly decrease, and the transcript level of the sex pheromone biosynthesis related genes HaPKA and HaACC is also significantly reduced. It is shown that HaPburs the genes participate in PG morphogenesis and regulate sex pheromone biosynthesis through a cAMP-PKA-ACC regulatory axis, thereby affecting the reproductive communication of the Helicoverpa armigera. The application provides a novel target and technical scheme for green and efficient prevention and control of the Helicoverpa armigera, and has important research and development value and application prospect.

The invention relates to the technical field of regenerative medicine application, in particular to a serum-free induction culture medium with definite components for promoting adipose tissue-derived cells to be differentiated into dermal papilla cells, an induction method and application. The invention relates to a method for promoting adipose tissue-derived cells to differentiate into dermal papilla cells, which is characterized in that the adipose tissue-derived cells are induced and cultured by using a serum-free induction culture medium, so that the adipose tissue-derived cells are differentiated into the dermal papilla cells. The formula of the serum-free induction culture medium comprises hepatocyte growth factors, hydrocortisone, bone morphogenetic protein 2, BIO and basic fibroblast growth factors. Based on the differentiation potential of adipose tissue-derived mesenchymal cells and the function maintenance mechanism of dermal papilla cells, a serum-free induction system with definite components is constructed, and directional differentiation is realized. According to the technical scheme, the technical problem that a method for inducing the adipose-derived mesenchymal cells to be directionally differentiated into the dermal papilla cells with the effective hair follicle regulation function is lacked in the prior art can be solved, and the method has ideal application and popularization potential.

The invention relates to a method for differentiating chimeric antigen receptor pluripotent stem cells into immune cells and application. The CAR-hPSCs with anti-FITC scFv are obtained by means of a CRISPR / Cas9 technology, then the CAR-hPSCs are differentiated into CAR-PMNs or CAR-NK by clearly combining chemical components with morphogenetic factors in a specific stage, various immune cells with excellent performance are obtained more simply, more accurately and more reliably, the production cycle can be shortened, the yield of target products is increased, the production cost is effectively reduced, and the immunotherapy effect is improved. The CAR-PMNs or CAR-NK can specifically recognize tumor cells highly expressed by folic acid receptors in a targeted manner through the bridging effect of the FITC-folic acid dual aptamers, the tumor cells are effectively killed, meanwhile, off-target toxic and side effects on normal tissues are avoided, and the effect of improving the treatment accuracy and the treatment effect of immune cells is achieved.

A method of modeling cell movement to predict a cellular process, including: providing a cell set including a plurality of cells, in which each cell within the cell set includes an assigned value and an assigned governing behavior; executing a self-sorting process of the plurality of cells within the cell set based on the assigned value and the assigned governing behavior of each of the plurality of cells, in which during the sorting process the cell set includes a plurality of intermediate arrangements of the plurality of cells within the cell set; determining an evolution of the intermediate arrangements of the plurality of cells during the sorting process; and analyzing the evolution of the intermediate arrangements and a final arrangement of the plurality of cells.

This invention relates to an indoor closed-loop Sargassum culture system and method. The sterilization chamber is an opaque chamber containing a sterilization device and a first pipeline. The first pipeline has a return branch, a first output branch, and a control valve at its upper end. The regulating chamber has a second pipeline and a carbon dioxide regulating system, with the second pipeline connected to the culture chamber. The pH value of the water is controlled by the carbon dioxide regulating system, which regulates the carbon dioxide input. The culture chamber contains a substrate and an oxygen regulating system. A lighting system is located above the culture chamber. The substrate has a height-adjustable telescopic rod, and the Sargassum algae are placed on the telescopic rod. The water temperatures in both the regulating and culture chambers are regulated by a temperature regulating system. This invention provides large-volume, closed-loop culture, simulating the wild growth mode and environment of Sargassum algae, enabling rapid growth, development, and morphogenesis of the algae.

A method of modeling cell movement to predict a cellular process, including: providing a cell set including a plurality of cells, in which each cell within the cell set includes an assigned value and an assigned governing behavior; executing a self-sorting process of the plurality of cells within the cell set based on the assigned value and the assigned governing behavior of each of the plurality of cells, in which during the sorting process the cell set includes a plurality of intermediate arrangements of the plurality of cells within the cell set; determining an evolution of the intermediate arrangements of the plurality of cells during the sorting process; and analyzing the evolution of the intermediate arrangements and a final arrangement of the plurality of cells.

To provide pharmaceutical compositions for osteoarthritis treatment, comprising immune-tolerized fetal stem cell-derived extracellular vesicles containing various components that promote hyaline cartilage regeneration and relieve inflammation at higher levels than those stem cell-derived extracellular vesicles obtained under general culture conditions, and thus exhibiting superior hyaline cartilage regeneration and anti-inflammatory efficacy as well as methods for manufacturing the extracellular vesicles and compositions for osteoarthritis treatment using the extracellular vesicles.SOLUTION: These extracellular vesicles derived from immune-tolerized fetal stem cells contain fibroblast growth factor-2 (FGF-2), fibroblast growth factor-7 (FGF-7), insulin-like growth factor-1 (IGF-1), transforming growth factor-beta 1 (TGF-β1), bone morphogenetic protein-4 (BMP-4), bone morphogenetic protein-5 (BMP-5), and bone morphogenetic protein-7 (BMP-7) and HLA-G protein.SELECTED DRAWING: Figure 1

The application provides a bone nodule organoid and a preparation method and application thereof, the bone nodule organoid comprises a microsphere carrier system, the microsphere carrier system is made of CGRP, BMP-2, bone marrow mesenchymal stem cells and GelMA-HA-NB hydrogel by a light curing 3D printing technology, the microsphere carrier system is mixed with GelMA hydrogel containing SP to be crosslinked and solidified to form the bone nodule organoid, and the bone nodule organoid is used for in-vivo transplantation to stimulate endogenous bone tissue rapid regeneration;The bone nodule organoid has the ability of two-phase delivery of bioactive factors, different bioactive factors can realize time sequence, differential low dose, slow and continuous release, so that the healing speed and bone repair effect of large-size bone defect are accelerated in a divide-and-rule manner.The application also provides a bioactive factor composition of a bionic bone nodule tissue neurotrophic factor and a physiological dose of bone morphogenetic protein, the composition releases the bone marrow mesenchymal stem cell osteogenic differentiation in the bone nodule organoid according to signal time sequence.

The present application relates generally to the field of bone morphogenetic protein (BMP) antagonists (soluble ALK6 fusion proteins), compositions thereof, and methods for treating degenerative and / or demyelinating diseases of the nervous system.

The application provides a self-development method and system based on a morphogenetic field, and a storage medium. The self-development method comprises the following steps: receiving a user intention, converting the user intention into a structured intention message; generating a functional semantic manifold and a mixed manifold space, and constructing a morphogenetic field; determining an implementation mode of a task target; decomposing the task target into a plurality of design tasks, and determining a design task of each morphogenetic engine; for each local state field, a corresponding morphogenetic engine is used to solve it; determining whether a cooperative stable state is reached among the plurality of morphogenetic engines; after the cooperative stable state is reached, collecting achievement data of the morphogenetic engine, and generating a standardized delivery package. Through intention analysis and construction of a morphogenetic field, the hardware and software implementation mode of the task target is objectively determined, the subjective bias of manual disassembly is eliminated, and the delivery process from abstract intention to standardized engineering products is realized.

This invention provides a compound preparation for promoting fracture regeneration and healing, and its preparation method. The compound preparation contains bioactive peptides, active ingredients from traditional Chinese medicine, angiogenesis-promoting components, bone morphogenetic proteins, chondroitin sulfate, vitamin D3, and alginate, among other active ingredients. The active ingredients from traditional Chinese medicine include various raw materials such as puerarin. The bioactive peptides and angiogenesis-promoting components are specifically formulated. During preparation, the bioactive peptides and active ingredients from traditional Chinese medicine are microencapsulated, the angiogenesis-promoting components are made into a nanoemulsion, and the bone morphogenetic proteins are freeze-dried, ensuring the stability and bioavailability of these active ingredients, thereby further enhancing the efficacy of the preparation. This compound preparation not only effectively promotes angiogenesis and bone tissue repair at the fracture site but also accelerates the fracture healing process and reduces patient pain. It demonstrates significant advantages in promoting fracture regeneration and healing, providing patients with a more efficient and safer treatment option.

The present invention relates to a ground extracellular matrix (ECM) produced by a human stem cell line with chondrogenic activity, wherein the human stem cell line is genetically modified to overexpress Bone Morphogenetic Protein-2 (BMP-2). The invention further relates to a composition comprising said extracellular matrix, and a gel-like substrate or precursor thereof. The invention further relates to a kit comprising said composition. The invention further relates to a method of manufacturing said composition. The invention further relates to a method for bone and / or bone marrow formation in vivo. The invention further relates to a method for testing a candidate pharmaceutical product. The invention further relates to a method for identifying treatment responders. The invention further relates to said composition for use in medicine.

The present invention relates to a method for assessing atrial fibrillation in a subject, said method comprising the steps of determining the amount of BMP10 in a sample from the subject, and comparing the amount of BMP10 to a reference amount, whereby atrial fibrillation is to be assessed. Moreover, the present invention relates to a method for diagnosing heart failure based on the determination of BMP10 in a sample from a subject. Further, the present invention relates to a method for predicting the risk of a subject of hospitalization due to heart failure based on the determination of a BMP10-type peptide in a sample from a subject. The present invention further pertains to antibodies which bind to one or more BMP10-type peptides such as NT-proBMP10.

The present application relates to the technical field of regenerative medicine application, and particularly relates to a serum-free induction medium with clear components for promoting differentiation of adipose-derived mesenchymal cells into dermal papilla cells, and an induction method and application. A method for promoting differentiation of adipose-derived mesenchymal cells into dermal papilla cells, which is to use a serum-free induction medium to induce culture of adipose-derived mesenchymal cells, so that the adipose-derived mesenchymal cells are differentiated into dermal papilla cells. The formula of the serum-free induction medium comprises hepatocyte growth factor, hydrocortisone, bone morphogenetic protein 2, BIO, and basic fibroblast growth factor. The present application is based on the differentiation potential of adipose-derived mesenchymal cells and the mechanism of maintaining the function of dermal papilla cells, and constructs a serum-free induction system with clear components to realize directional differentiation. The technical scheme can solve the technical problem that there is no method for inducing directional differentiation of adipose-derived mesenchymal cells into dermal papilla cells with effective hair follicle regulation function in the prior art, and has ideal application and popularization potential.

The invention discloses a device and a method for researching interaction of carbon nutrition and mineral nutrient elements of crops, the device comprises a culture dish and a middle cover plate, the culture dish comprises a dish body and a dish cover, the middle cover plate is provided with an upward support and a downward foot stool, the foot stool of the middle cover plate is supported in the dish body of the culture dish, and the dish cover is provided with a water inlet and a water outlet. And the bracket of the middle cover plate upwards supports the dish cover of the culture dish. According to the device and the method, photosynthesis can be well inhibited on the basis of not influencing plant photomorphological establishment, endogenous carbon nutrient substances of crops can be consumed in a short time, and the plants can quickly respond to exogenous carbon and nitrogen supply. Meanwhile, the device and the method can be expanded and applied to research on interaction of carbon nutrition and other mineral nutrient elements of other crops such as wheat, soybean and corn.

The invention discloses an Anemonium aureum SkORC3 gene as well as an expression protein and application thereof, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the SkORC3 gene is as shown in SEQ ID NO. 1, and the amino acid sequence of the protein coded by the SkORC3 gene is as shown in SEQ ID NO. 2. According to the invention, the gene is constructed into an overexpression vector and is converted into arabidopsis thaliana, so that the key function of the SkORC3 in regulating and controlling the morphogenesis of plant leaves is disclosed for the first time. A transgenic arabidopsis thaliana plant over-expressing the gene shows remarkable rosette leaf dysplasia, and the specific phenotypes comprise double main vein bifurcated heart-shaped leaves, symmetrical twin leaves at the top end of a petiole, uniaxial special-shaped leaves and the like. The invention provides a new gene resource and tool for molecular mechanism research of plant leaf development, and has potential application value in the field of ornamental plant leaf type molecular breeding.

This method for producing mesenchymal cells of the ventral hindgut mesoderm comprises: culturing pluripotent stem cells in an induction medium A containing a TGF-β inhibitor, a Wnt agonist, and a fibroblast growth factor to induce differentiation into epiblast-like cells; culturing the epiblast-like cells in an induction medium B containing a Wnt agonist, a fibroblast growth factor, and retinoic acid to induce differentiation into caudal epiblast-like cells; culturing the caudal epiblast-like cells in an induction medium C containing a Wnt agonist, a bone morphogenetic protein, and a TGF-β family member to induce differentiation into posterior primitive streak cells; and culturing the posterior primitive streak cells in an induction medium D containing a Wnt agonist, a fibroblast growth factor, a bone morphogenetic protein, and a hedgehog signaling agonist to induce differentiation into mesenchymal cells of the ventral hindgut mesoderm.

Disclosed are compositions containing human pancreatic islet organoids. The disclosed human pancreatic islet organoid culture systems mimic the morphogenic event in the natural islet development process, thereby enabling spontaneous generation of islet structure with high efficiency and preserving crucial islet structure. Also disclosed are methods of generating human pancreatic islet organoids, wherein the methods require a six-stage culture system. Further disclosed are methods using the in vitro generated organoids and compositions thereof. The in vitro generated organoids are particularly suited for research purposes, providing optimized cell models for the study of pancreatic islet biology, endocrine biology and metabolic disease pathology. The in vitro generated organoids are also particularly suitable for biomarker identification, and evaluation of new molecules and existing therapies for treating metabolic and / or endocrine diseases.

There is provided a method for efficient differentiation induction from human pluripotent stem cells into hypothalamic neurons. Also, provided is a method for constructing, from human pluripotent stem cells, a cellular structure in which hypothalamic tissue and pituitary tissue are integrated. A cellular structure including hypothalamic tissue is obtained by a method including the steps of: culturing an aggregate of human pluripotent stem cells in suspension in a medium containing a low concentration of a bone morphogenetic protein signal transduction pathway activating substance and a low concentration of a substance acting on the Shh signaling pathway; and further culturing the cell aggregate obtained in the step in suspension in a medium containing a low concentration of a substance acting on the Shh signaling pathway.

This invention discloses the application of oxalic acid in the preparation of drugs against Riemerella anatipestifer infection, belonging to the field of biomedical technology. This invention is the first to discover that oxalic acid can inhibit the growth of Riemerella anatipestifer (RA). After treatment with oxalic acid, the bacterial morphology changes significantly, RA biofilm formation is inhibited, and existing biofilms are destroyed. This indicates that oxalic acid can inhibit related virulence phenotypes, exerting an inhibitory effect on the key steps of RA infection—adhesion and colonization—thereby preventing and controlling RA disease. This invention provides a new potential drug for the development of drugs against Riemerella anatipestifer and lays a theoretical foundation.

Methods and compositions for generating transgene-free plants are described. In particular, the transgene-free plants are regenerated by transient expression of morphogenic regulators in a plant cell that is precisely edited using a gene-editing methodology. Also, provided are transformed plants, plant cell, tissues, and seeds in which a target gene is edited, but non-transgenic.