70 results about "Myogenic cell" patented technology

The invention discloses a method for promoting skeletal muscle development and application, and belongs to the technical field of biology. The invention provides a method for promoting skeletal muscle development, an AMPK / p38MAPK pathway is regulated and controlled by activating PPARalpha, the way of activating the PPARalpha is to give a PPARalpha agonist GW7647, and the regulation and control of the AMPK / p38MAPK pathway is shown as reduction of AMPK and P38 phosphorylation levels. Experiments show that the expression of MyoD, MyoG and Pax7 in skeletal muscle can be remarkably improved by activating PPARalpha, the phosphorylation level of AMPK and P38 is inhibited, and therefore muscle fiber proliferation and myoblast proliferation are promoted. The technology can be applied to livestock breeding to improve meat yield or develop drugs for treating skeletal muscle atrophy, and has remarkable economic and clinical values.

The invention provides a method for producing cell culture meat through 3D scaffold-free co-culture of pseudosciaena crocea stem cell spheres, and belongs to the technical field of preparation of cell culture meat. A method for producing cell culture meat by 3D scaffold-free co-culture of pseudosciaena crocea stem cell spheres comprises the following steps: mixing and inoculating pseudosciaena crocea myoblasts and pseudosciaena crocea precursor adipocytes into a cell culture plate subjected to anti-adhesion treatment for proliferation culture, spontaneously gathering to form cell spheres, and carrying out differentiation culture to obtain the cell culture meat. According to the co-culture mode provided by the invention, high-efficiency myogenic differentiation and adipogenic differentiation of myoblasts and precursor adipocytes of the large yellow croakers can be realized through proliferation culture and differentiation culture, and an in-vivo microenvironment can be effectively simulated, so that cultured meat similar to a traditional meat product in texture and flavor is produced.

The invention relates to application of a yeast peptide-derived polypeptide in promoting proliferation and differentiation of myoblasts and a composition, and belongs to the technical field of biological medicines and nutrition and health care. The amino acid sequence of the polypeptide comprises SEQ ID No.1 (LGGPLL), SEQ ID No.2 (PQAVTI), SEQ ID No.3 (VASGGL) or SEQ ID No.4 (LGPTGISM, and the polypeptide can be used for remarkably promoting the proliferation and the cell cycle progress of C2C12 myoblasts and up-regulating the expression level of myogenic protein. The polypeptide can be used as an active component to be applied to development of anti-sarcopenia related medicines or functional food compositions. Cell experiments prove that the polypeptide has a good muscle promoting function and is suitable for intervention of senile muscle loss such as sarcopenia and the like.

The invention discloses application of an FGF9 gene in regulation and control of growth and development of chicken skeletal muscles, and further discloses application of an overexpression vector of the FGF9 gene or an interference fragment of the overexpression vector in promotion or inhibition of proliferation and differentiation of chicken myoblasts. The invention also discloses a specific primer pair for detecting the expression quantity of the FGF9 influencing the growth and development of the chicken skeletal muscle. The invention also discloses a detection kit and a detection method thereof. The detection kit provided by the invention can be used for detecting the expression quantity of the FGF9 gene in chicken muscle tissues and myoblasts, and the detection method is simple and quick. The FGF9 gene can be used as an important gene for researching a chicken skeletal muscle growth and development regulation theory.

The invention discloses a CircSESN1 gene as well as an overexpression vector, a specific primer pair, a detection kit and application of the CircSESN1 gene. The invention also discloses application of the CircSESN1 gene, an overexpression vector, the specific primer pair or the fluorescent quantitative detection kit in regulation and control of growth and development of chicken skeletal muscle. The invention also discloses a method for inhibiting chicken myoblast proliferation and / or promoting differentiation. The detection kit provided by the invention can be used for detecting the expression quantity of the CircSESN1 gene in chicken muscle tissues and myoblasts, and the detection method is simple and quick. The gene can be used as an important non-coding RNA for researching a regulation theory of growth and development of chicken skeletal muscles.

The present invention provides a production method for a three-dimensional muscle tissue including the steps of: preparing an approximately rectangular first cell module containing skeletal myoblasts in a hydrogel, and having a plurality of approximately rectangular holes parallel to each other, and an approximately rectangular second cell module containing skeletal myoblasts in a hydrogel, and having a plurality of approximately rectangular holes parallel to each other at positions different from those of the first cell module in a vertical direction; alternately stacking the prepared first cell module and the prepared second cell module to obtain a stack; subjecting the skeletal myoblasts contained in the obtained stack to proliferation culture; and inducing the proliferated skeletal myoblasts to differentiate into myotubes.

The present invention relates to an albumin nanocomposite comprising a phytochemical, and a composition for improving muscle diseases comprising the same, and more specifically, to an albumin nanocomposite comprising a phytochemical, the present invention relates to an albumin nanocomposite containing phytochemicals, and more specifically, to an albumin nanocomposite containing phytochemicals, which exhibits the effects of inhibiting muscle reduction caused by oxidative stress or inflammatory response and promoting the differentiation of myoblasts into muscle cells. The albumin nanocomposite containing the phytochemicals can target and induce immune cells of active oxygen and inflammatory reaction and effectively deliver the phytochemicals, so that muscle cell atrophy is inhibited and differentiation is promoted by adjusting a muscle reduction signal mechanism induced by the active oxygen and the inflammatory reaction, and further muscle reduction can be improved, so that the curative effect of the albumin nanocomposite is improved. The muscular dystrophy is prevented and treated. Therefore, the composition containing the albumin nanocomposite containing phytochemicals of the present invention can be provided as a composition for improving muscle diseases.

This invention relates to the application of oridonin in the preparation of agents that improve muscle function, specifically by improving skeletal muscle dysfunction, enhancing insulin sensitivity, promoting myoblast differentiation into myotube cells, and / or increasing myoblast insulin sensitivity. Using an in vitro C2C12 myoblast model, this invention demonstrates that oridonin can promote myoblast differentiation and growth and increase C2C12 myoblast insulin sensitivity. Through an obesity-induced skeletal muscle dysfunction model, it is demonstrated that oridonin can improve skeletal muscle dysfunction and insulin resistance by increasing muscle strength, muscle endurance, muscle mass, and reducing muscle lipid accumulation. This invention provides new insights for drug research on improving skeletal muscle dysfunction and enhancing insulin sensitivity, and also enriches the pharmacological system of oridonin.

The invention discloses application of glutamic acid and alpha-KG in promoting differentiation and fusion of skeletal muscle myoblasts. In particular discloses application of glutamic acid and / or alpha-ketoglutaric acid in promoting myoblast fusion. The invention also discloses a method for promoting myoblast fusion. The method comprises the step of contacting myoblasts with glutamic acid and / or alpha-ketoglutaric acid. It is found for the first time that glutamic acid and alpha-KG have the effect of promoting myoblast differentiation and / or fusion, and glutamic acid and alpha-KG can be prepared into feed, feed additives, myoblast fusion promoters and other products to be used for promoting animal muscle development and breeding high-yield and high-quality meat animal products. Experiments show that by adding a proper amount of glutamic acid and alpha-KG in the muscle cell differentiation process, the fusion rate of mouse C2C12 cells and sheep skeletal muscle myoblasts can be remarkably increased, the number of formed myotubes is increased, and the differentiation level of the two cells is promoted. The method has a wide application prospect in the fields of livestock breeding and the like.

The present invention relates to a composition for the improvement and treatment of muscle diseases and cachexia, wherein diflunisal has the effect of promoting the differentiation and proliferation of myogenic cells, and further has the effect of improving and treating cachexia induced by chronic diseases such as muscle diseases or cancer through effects such as increased muscle mass and recovery of muscle strength.

The application discloses a kind of construction method and application of seawater fish muscle stem cell line, culture medium with basic medium as background medium;Culture medium includes LIF growth factor, EGF growth factor and leopard bristletooth-derived FGF2 protein;The final concentration of leopard bristletooth-derived FGF2 protein is 20~30 ng / mL, 30~45 ng / mL, 45~55 ng / mL or 55~70 ng / mL. Through the medium, immortalized seawater fish muscle stem cell line with good stability, cell division vigorous, short subculture time can be constructed, and can be stably subcultured for more than 50 generations. It provides raw materials for exploring the function of myoblasts, gene regulation mechanism, and provides the prerequisite for in vitro verification experiment for further exploring the influence of seawater fish muscle stem cells on growth rate, and lays a foundation for good strain breeding of seawater fish.

The invention discloses application of deaminotyrosine in improvement or prevention and treatment of muscular atrophy, and relates to the technical field of biological medicines. The invention finds that in the cellular level, DAT can effectively resist C2C12 myoblast senescence induced by etoposide; in a dexamethasone-induced C2C12 myotube atrophy model, DAT not only inhibits myotube diameter reduction from the form, but also down-regulates mRNA expression of key atrophy genes Atrogin-1 and MuRF-1 from the molecular level, and inhibits excessive activation of a ubiquitin-proteasome system; in-vivo animal experiments prove that DAT can reverse aging-related dyskinesia of rapidly-aged SAMP8 mice, and gait parameters of the rapidly-aged SAMP8 mice are all remarkably improved. According to the invention, a complete evidence chain from cells to the whole is constructed, and the DAT is proved to improve the senescent skeletal muscle atrophy through multiple ways of intervening cell senescence, antagonizing protein degradation, improving muscle microenvironment and the like, and shows important potential application value.

To provide methods for producing an artificial skeletal muscle tissue from pluripotent stem cells.SOLUTION: The present invention describes a method for preparing an engineered skeletal muscle tissue, as well as skeletal myoblasts, skeletal myotubes, and satellite cells, a medium used being serum-free and different chemicals and their concentrations, as well as the physical stimuli, being defined. Additionally, the methods described herein are performed without transfection of human cells with transgenes. The engineered skeletal muscle cells exhibit myoblast-, myotube-, or satellite cell-specific genetic markers, demonstrating efficient differentiation of these cell types. Despite its engineered production, the skeletal muscle tissue possesses excellent stimulus-dependent contractility and exhibits contractions in response to different stimulation frequencies.SELECTED DRAWING: None

The invention discloses an application of a YTHDC1 gene in regulating myoblast proliferation or differentiation and skeletal muscle development. The influence of the YTHDC1 gene on proliferation and differentiation of myoblasts is researched by inhibiting and overexpressing the YTHDC1 gene in the myoblasts, and it is found that the overexpressed YTHDC1 gene has the functions of promoting differentiation of the myoblasts and inhibiting proliferation of the myoblasts; myoblast proliferation can be promoted and differentiation of myoblasts can be inhibited by inhibiting YTHDC1 gene expression, and muscle growth of mice after birth can be promoted. And further research finds that the YTHDC1 gene affects muscle development by regulating and controlling the variable splicing process of muscle development related genes such as AkaP13, Smarca2, Tnnt3 and Neb. Experimental results provide a research basis for skeletal muscle growth and development regulation and control, and also provide theoretical support for improving meat product quality.

The invention relates to application of an FGFBP2 gene in regulation and control of growth and development and cell cycle of chicken skeletal muscle. The inventor finds the existence of the FGFBP2 gene in early-stage work of a team, and also finds that the expression quantity difference of the gene in muscular tissues of chickens in different development stages and varieties is obvious. In order to further reveal the effect of the FGFBP2 gene in the growth and development process of chicken skeletal muscles, the FGFBP2 gene is overexpressed in chicken myoblasts and AA broiler chickens respectively by using an overexpression technology, and detection finds that the overexpressed FGFBP2 gene can promote the proliferation of myoblasts. The discovery that the FGFBP2 gene can regulate and control the proliferation function of the chicken skeletal muscle cells provides a new theoretical basis for revealing the development of the chicken skeletal muscle and improving the meat production character, and provides a new thought for improving the meat production performance of livestock.

The present disclosure relates to an albumin nanocomposite comprising a phytochemical and a composition for muscle disease comprising the same, and more specifically, to an albumin nanocomposite comprising a phytochemical that exhibits an effect of inhibiting muscle loss caused by oxidative stress or inflammatory response and promoting the differentiation of myoblasts into muscle cells. The albumin nanocomposite comprising the phytochemical effectively delivers the phytochemical by targeting immune cells that induce reactive oxygen species and inflammatory responses. Accordingly, by regulating muscle-loss signaling pathways induced by reactive oxygen species and inflammatory responses, the nanocomposite inhibits muscle cell atrophy and promotes differentiation. Therefore, a composition comprising the albumin nanocomposite comprising the phytochemical according to the present disclosure can be provided as a composition for improving, preventing, or treating muscle diseases.

The invention discloses a method for separating, identifying and proliferating myoblasts of small-tailed han sheep, which comprises the following steps: (1) taking and treating skeletal muscle tissues: selecting healthy small-tailed han sheep, collecting the skeletal muscle tissues under sterile conditions, washing and cutting into pieces to obtain tissue blocks; (2) primary myoblast isolated culture: digesting, filtering and centrifuging the obtained tissue block, and culturing; (3) myoblast purification: purifying the cultured primary myoblasts to obtain purified myoblasts; (4) identifying myoblasts: identifying the obtained purified myoblasts; and (5) proliferation of myoblasts: after identification is completed, carrying out cell proliferation on the purified myoblasts obtained in the step (3) by utilizing a proliferation culture medium containing vitamin E. According to the method provided by the invention, high-purity and high-activity myoblasts can be obtained; vitamin E is utilized, so that the proliferation capacity of myoblasts can be remarkably improved, and the cell differentiation function of the myoblasts is not influenced.

The invention discloses the technical field of animal cell culture and cell culture meat, and provides a food-grade natural compound combination method for promoting efficient myogenic differentiation in vitro. According to the method disclosed by the invention, a combination of xanthophyll and arachidic acid is added into a basic myogenic differentiation induction culture medium to form a myogenic differentiation promoting culture medium. According to the myogenic differentiation culture medium system, the myogenic differentiation efficiency of the myogenic cells can be improved from about 23% to 45% or above, the myogenic fusion efficiency can be improved from about 18% to 33% or above, the problems that the in-vitro differentiation efficiency of the myogenic cells is low, the content of key proteins such as myosin is insufficient and the like are effectively solved, and the myogenic differentiation culture medium system is suitable for large-scale popularization and application. And a feasible scheme is provided for large-scale muscle fiber production of cell culture meat.

The present invention relates to a phytochemical-containing albumin nanocomplex and a composition containing the same for improving muscle diseases. More specifically, the present invention relates to a phytochemical-containing albumin nanocomplex that has the effect of suppressing muscle loss caused by oxidative stress or inflammatory responses and promoting the differentiation of myoblasts into muscle cells. The phytochemical-containing albumin nanocomplex effectively delivers phytochemicals to immune cells that induce reactive oxygen species and inflammatory responses, thereby regulating the muscle loss signaling mechanism induced by reactive oxygen species and inflammatory responses, suppressing muscle cell atrophy, and promoting differentiation. This can improve muscle loss and prevent and treat muscle atrophy. Therefore, a composition containing the phytochemical-containing albumin nanocomplex of the present invention can be provided as a composition for improving muscle diseases.

Disclosed herein are systems and methods for testing effects of simulated microgravity on muscle structures. The system includes a clinostat and an adapter that is receivable into the clinostat. The adapter includes a housing that defines at least one receptacle. The system further includes an assembly having a substrate and a plurality of fibrils deposited on the substrate. The plurality of fibrils comprise collagen and extend along an axis. The assembly is receivable into a first receptacle of the at least one receptacle. The plurality of fibrils can have myoblasts thereon. Operation of the clinostat simulates microgravity. Test substances can screened using the muscle structures to determine effects on myogenesis.

The invention also discloses application of the bovine-derived SYPL2 gene in regulation and control of animal muscle development and regeneration. According to the invention, the SYPL2 gene is found to be a key regulatory factor for muscle development, and an intervention strategy based on the gene is developed, so that a part of technical problems are effectively solved, and an innovative solution is provided for livestock breeding and muscle regeneration medicine. The invention discovers that the SYPL2 gene has high specific expression in bovine skeletal muscle tissues for the first time, and has the capability of promoting proliferation and differentiation of bovine myoblasts cultured in vitro. More importantly, when the bovine SYPL2 gene overexpression plasmid is locally injected into a mouse with acute muscle injury, a remarkable muscle injury regeneration and repair treatment effect is shown, so that a brand new target and a solution are provided for livestock molecular breeding and muscle regeneration medicine; and an important basis is provided for improving the production efficiency of animal husbandry and developing a novel muscle repair therapy.

The present invention relates to the use of a hemp seed extract for preventing or ameliorating muscle diseases by promoting myoblast differentiation or muscle regeneration. In the present invention, the hemp seed extract may exhibit preventive or therapeutic effects on various muscle diseases by increasing muscle mass, strengthening muscle power, improving muscle function, and enhancing exercise performance, and also has an effect of improving muscle function in healthy individuals in addition to treating muscle diseases.

The present invention relates to a composition for preventing, alleviating, or treating muscle diseases, the composition comprising an NFAT inhibitor as an active ingredient. An NFAT inhibitor (VIVIT peptide) comprising the amino acid sequence of SEQ ID NO: 1 according to the present invention was found to: alleviate the inhibition of muscle differentiation by suppressing NFAT signaling pathway activation induced by excessive calcium exposure, and increase the expression levels of muscle differentiation-related proteins in C2C12 myoblasts and mouse muscle-derived myoblasts. Thus, the NFAT inhibitor can be effectively used as a composition for preventing, alleviating, or treating muscle diseases.

The present application relates to the technical field of muscle chip, in particular to a vascularized muscle model and its preparation and application. The present application first mixes myoblasts, endothelial cells, cell culture solution and extracellular matrix substitute to obtain a suspension; then the suspension is placed in a pretreated muscle chip to induce differentiation to obtain a vascularized muscle model. The present application realizes the co-culture of muscle and blood vessels in the chip, which is more bionic muscle microenvironment, so that the formed tissue is more similar to in vivo in terms of morphological structure and key physiological function, and can be more effectively applied to the basic research and clinical drug screening of muscle injury related diseases, so that the obtained research results are more accurate and reliable. The vascularized muscle model prepared by the present application can be widely applied to the basic research and drug evaluation system of muscle related diseases.

The application discloses CircSESN1 a gene, an overexpression vector thereof, a specific primer pair, a detection kit and application thereof. CircSESN1 The application also discloses application of the gene, the overexpression vector, the specific primer pair or the fluorescent quantitative detection kit in regulation of chicken skeletal muscle growth and development. CircSESN1 The application further discloses a method for inhibiting proliferation and / or promoting differentiation of chicken myoblasts. The detection kit provided by the application can be used for detecting

This invention discloses a method for constructing and applying a marine fish muscle stem cell line. The culture medium uses basal medium as the background medium and contains LIF growth factor, EGF growth factor, and FGF2 protein derived from the leopard-gill sea bass. The final concentration of FGF2 protein from the leopard-gill sea bass is 20-30 ng / mL, 30-45 ng / mL, 45-55 ng / mL, or 55-70 ng / mL. Passaging using this medium can construct a stable, rapidly dividing, and short-passaging immortalized marine fish muscle stem cell line, capable of stable passage for more than 50 generations. This provides raw materials for exploring the function and gene regulation mechanisms of myoblasts, and provides in vitro verification experimental conditions for further research on the effects of marine fish muscle stem cells on growth rate, laying the foundation for the breeding of superior marine fish species.

The application discloses functions and application of KIF5B gene in regulating physiological activity of myoblasts, and belongs to the technical field of animal cell culture. By investigating influences of KIF5B knockdown and overexpression on proliferation of myoblasts, it is found that KIF5B knockdown can significantly inhibit DNA synthesis speed of myoblasts, thereby inhibiting proliferation. In addition, overexpression of KIF5B can significantly inhibit expression of MYOD and MYOG, improve DNA synthesis speed of myoblasts, thereby significantly improving proliferation rate of myoblasts. The application provides an important key molecular target and technical support for efficient and rapid culture of myogenic cells.

The application belongs to the technical field of poultry breeding, and discloses siRNA for targeted inhibition of chicken ULK2 expression and application thereof; the siRNA comprises ULK2-1170; the siRNA for targeted inhibition of chicken ULK2 gene expression can effectively reduce the expression of the chicken ULK2 gene after being transferred into chicken primary myoblast cells, the inhibition efficiency is more than 50%, and the knockdown efficiency is very high; the siRNA can be used for identifying gene functions by specifically knocking down the expression of the chicken ULK2 gene in vitro, and can be applied to genetic selection of high-quality broiler leg muscle development as a molecular marker, and has great economic value and scientific research value.

The application discloses a use and application based on CDKN1A influence on proliferation and differentiation of goat skeletal muscle, relates to the field of agricultural genetic engineering, and discloses that iRNA-seq and RNA-seq technology reveals mRNA expression maps of the longest dorsal muscle of 5-month-old Hainan black goats and 24-month-old Hainan black goats in two development stages, and CDKN1A with differential expression is screened out; the CDKN1A gene is used for negatively regulating proliferation of goat myoblasts; and the application finds that CDKN1A has a regulation effect on proliferation and differentiation of skeletal muscle cells of Hainan black goats, and can be applied to development and utilization of germplasm resources of the breed and seed selection and breeding.

The invention belongs to the technical field of bioengineering, and particularly relates to a long-chain non-coding RNA (Ribonucleic Acid) and application thereof in regulation and control of growth and development of chicken skeletal muscle. An animal individual level LncIGFBP5 overexpression model is constructed, gastrocnemius muscle cells are taken as a research object, and the glucolipid metabolism function of the liver is influenced by inhibiting proliferation and differentiation of muscle cells, promoting muscle fiber type conversion, inhibiting growth and development of gastrocnemius muscle and quadriceps femoris muscle and promoting the gastrocnemius muscle glycogen accumulation and adiposis process. The growth performance and metabolic balance of the broiler chicken are further influenced; myoblasts are used as research objects, an LncIGFBP5 overexpression and interference model is constructed, overexpression promotes myoblast proliferation, interference promotes myoblast differentiation, and overexpression promotes expression of fast muscle marker genes and slow muscle marker genes at the same time; a theoretical basis is provided for understanding the effect of the LncIGFBP5 in muscle development and metabolism regulation, and a potential target is provided for poultry meat quality improvement, molecular breeding and nutrition regulation.