111 results about "Myogenic cell" patented technology

An analgesic benefit is realized by continuously supplying a peptide in vivo that activates an opioid receptor or that interferes with the binding of substance P to its receptors. The long-term, continuous provision of such a peptide can be accomplished by (a) transducing myogenic cells with DNA expressing the peptide and (b) administering the transduced myogenic cells to a patient, such that the cells continuously produce the peptide.

The present invention provides muscle-derived cells, preferably myoblasts and muscle-derived stem cells, genetically engineered to contain and express one or more heterologous genes or functional segments of such genes, for delivery of the encoded gene products at or near sites of musculoskeletal, bone, ligament, meniscus, cartilage or genitourinary disease, injury, defect, or dysfunction. Ex vivo myoblast mediated gene delivery of human inducible nitric oxide synthase, and the resulting production of nitric oxide at and around the site of injury, are particularly provided by the invention as a treatment for lower genitourinary tract dysfunctions. Ex vivo gene transfer for the musculoskeletal system includes genes encoding acidic fibroblast growth factor, basic fibroblast growth factor, epidermal growth factor, insulin-like growth factor, platelet derived growth factor, transforming growth factor-β, transforming growth factor-α, nerve growth factor and interleukin-1 receptor antagonist protein (IRAP), bone morphogenetic protein (BMPs), cartilage derived morphogenetic protein (CDMPs), vascular endothelial growth factor (VEGF), and sonic hedgehog proteins.

An improved device and method for extended repair and regeneration of muscle tissue. An exemplary device comprises (a) a scaffold comprising an ECM component; (b) a combination of growth factors such as VEGF and IGF; and (c) a population of myogenic cells. Implantation of the device leads to muscle regeneration and repair over an extended period of time.

Catheters and methods for their use are presented that provide automated delivery of cells to structures in the body such as degenerative or weak muscle. The catheters contain one or more sensing components and a system for delivery of cells such as autologous myogenic cells. The sensing component(s) triggers automatic discharge of the cells upon detection of a body surface that needs repair. The discharge of cells may occur through an automated lancing mechanism that alleviates operator error from manual injection timing. During use, an operator can position the catheter end to a structure in need of cellular repair, and the device can automatically discharge cells at a location and time as determined by conditions sensed at the catheter end. A catheter and system is particularly useful for repair of degenerative and or weak heart muscle, such as that found after a myocardial infarct. The catheters and methods of their use also may be used for cellular repair of other interior body structures, particularly muscles such as bladder, intestine, stomach and diaphram. The catheters provide greater use of myogenic cell therapy for heart disease, while avoiding complications of open heart surgery.

The object of the present invention is to provide a method of preparing excellent cultured muscle cells having high metabolic capacity and insulin responsiveness, and further provide a method for the measurement of sensitive metabolic capacity using the cells. Moreover, its purpose is to provide a culture system/culture apparatus that can smoothly translocate such highly advanced cultured muscle cells intact to activity evaluation systems of a number of drugs. Moreover, the object of the present invention is to provide cultured muscle cells that are very suitable for measurement of the membrane-translocation activity of GLUT4 in an extraneous stimulus-dependent manner such as insulin, etc., and to provide a method for the measurement of the membrane-translocation activity of GLUT4 using the cells. The present invention is a method of preparing myotube cells, comprising a step (1) of culturing myoblast cells, a step (2) of differentiation-inducing the myotube cells into the myoblast cells in a culture medium with a high content of amino acids, and a step (3) of applying an electric pulse to the differentiation-induced myotube cells, and a method for the measurement of insulin-dependent sugar uptake using the myotube cells prepared by said method, and relates to the method for the measurement, comprising applying insulin stimulation by culturing the cells in a culture medium containing insulin, culturing the cells in the culture medium further supplemented with sugar, and measuring the sugar uptake. Furthermore, the present invention relates to a differentiation-type culture myotube cell constitutively expressing a recombinant GLUT4 having a labeled substance at its extra-cellular site, which is prepared by co-culturing wild-type myoblast cells and recombinant myoblast cells constitutively expressing said recombinant GLUT4, and a method for the measurement of membrane-translocation activity of the recombinant GLUT4 using the cells, and particularly a method for the measurement of insulin-dependent sugar uptake activity.

The invention discloses a CHO cellar strain of high-effective expressive exogenous rhBMP2, which is characterized by the following: adopting CHO-dhfr- in the dihydrofolic acid reductase flaw-typed Chinese hamster ovary cell as host cell; infecting exogenous hBMP2 plasmid; adopting secretory expressive product to induce myoblast system C2C12 to display the activity to stimulate sclerotomal cell; obtaining single-clone strain of CHO (rhBMP-2) with the content of BMP2 at 7.83ug/24h*106; fitting for clinic treatment.

The invention discloses a production mold for porous reticular cultured meat with regularly arranged micro-columns and a production method of porous muscle tissue based on the mold. Myogenic cells, type I collagen, a DMEM medium containing phenolic red and a sodium hydroxide solution are mixed, a mixture containing cells is prepared, the mixture containing the cells is added into the mold, and hydrogel muscle tissue is cultured at 37 DEG C after 2 hours in an incubator containing 5% carbon dioxide. A growth medium is added, after the hydrogel muscle tissue is cultured for 1-3 d, the growth medium is changed into a differential medium, after the hydrogel muscle tissue is cultured in the differential medium for 5-7 d, the porous reticular muscle tissue is prepared, so that the hydrogel muscle tissue can contract between the micro-columns to form muscle bundles, contracting space around the micro-columns due to the muscle bundles can increase the diffusion of nutrients to the cells, wastedischarge is facilitated, spatial pattern of mechanical tension is controlled to guide the partial three-dimensional cell arrangement, differentiation is promoted, and the production of larger in vitro, in line with industrial production of cultured meat is realized.

The invention is directed to a method for producing an edible composition, comprising incubating a three-dimensional porous scaffold and a plurality of cell types comprising: myoblasts or progenitor cells thereof, at least one type of extracellular (ECM)-secreting cell and endothelial cells or progenitor cells thereof, and inducing myoblasts differentiation into myotubes.

The invention provides a drug for promoting myoblast proliferation and improving myoblast autophagy level, which is characterized in that the active ingredient of the drug is DNA tetrahedron. The invention also provides a new drug for promoting muscle regeneration, which is prepared by taking a DNA tetrahedron as an active ingredient and adding pharmaceutically acceptable auxiliary materials. Theinvention provides the new application of the DNA tetrahedron in preparation of muscle regeneration promoting drugs, and the DNA tetrahedron has a very good application prospect.

The invention relates to oyster mushroom galactomannan as well as a preparation method and an application thereof, and belongs to the field of preparation of effective components of edible mushrooms.The pleurotus ostreatus galactomannan is obtained by performing normal-temperature wall-breaking extraction on dried fruiting bodies of pleurotus ostreatus, and combining extrusion separation, proteinremoval and ion exchange chromatography. According to the wall breaking in combination with extrusion filtration, in the preparation method, the extraction time is shortened, the reagent consumptionis reduced, the energy is saved, and the polysaccharide yield is improved; and an adopted extrusion filtration technology is suitable for separation of a viscous solution, and the obtained oyster mushroom galactomannan has the functional activity of protecting myocyte oxidative damage, and can be used for preparing auxiliary medicines and health foods for protecting myocyte oxidative damage.

The present invention relates to establishing method of active cell strain for quantitative determination of bone morphogenetic protein (BMP). By means of the transgenic technology of molecular biology, partial promoter of BMP action target molecular osteocalcium together with leuciferinase report gene is transfered to myogenic cell C2C12, monoclonal cell strain to express leuciferinase report gene stably is obtained through screening and the reaction of the cell strains to BMP action is determined. Strain with high reaction strength to BMP stimulation and sensitive determination index is finally sorted. Specificity and stability test proves the stability and reliability of the cell strain and multiple BMP sample testing result shows that the action of BMP is correlated with leuciferinase activity linearly and directly. The cell strain of the present invention is suitable for quantitative determination of BMP activity.

The invention discloses a culture method for improving oxidative metabolic capability of chicken skeletal muscle cells, belonging to the technical field of biology. The method provided by the invention comprises the following steps: respectively inoculating separated and purified chicken skeletal muscle sarcoblasts and muscle-derived fibroblasts into an upper chamber and a lower chamber of a Transwell culture dish, wherein the chicken skeletal muscle sarcoblasts and the muscle-derived fibroblasts are separated by a PET membrane having a pore size of 1 mu m but share a myogenic differentiation culture medium with added H2O2; and performing myogenic differentiation induced culture for 7-10 days to obtain myotubes having an independent retraction capability, and performing low-temperature impact stimulation once every 8-12 hours in the culture period. According to the method provided by the invention, the mitochondrion content and transmembrane potential of the chicken skeletal muscle cells as well as the activity of aerobic metabolic enzymes can be obviously improved; and meanwhile, the activity of ATPase can be reduced. The method has the characteristics of short culture period and stable effect, and lays a foundation for researching a chicken skeletal muscle energy metabolism law, a fiber type formation mechanism and the like, thus having favorable research and application value.

The invention provides a making method of a three-dimensional vascularized musculocutaneous flap based on biological 3D printing. Coaxial biological 3D printing is utilized; a calcium chloride solution is taken as an inner layer; methacrylic anhydride gelatin, sodium alginate, polyethylene glycol diacrylate and vascular smooth muscle cells are mixed to serve as outer-layer biological ink; a printed scaffold is thoroughly cross-linked with blue light through calcium chloride; a three-in-one perfusate scaffold is finally formed, so that blood supply is established; vascular endothelial cells are poured into the scaffold; vascular cell components are simulated; fibroblasts, adipose tissue-derived stromal cells and skeletal muscle myoblasts are respectively inoculated on a three-layer scaffold; a fibroblast culture medium, an adipogenesis induction culture medium and a myoblast culture medium are respectively added and put into a reactor for culture; and the three-layer scaffold is folded and bond through rat tail collagen to form the stacked scaffold. According to the invention, integrated perfusion of blood flow and independent culture differentiation and assembly of different cell components are realized.