1766 results about "Cell strain" patented technology

The invention relates to a mouse anti-PBP2a hybridoma cell strain, a monoclonal antibody and application of the mouse anti-PBP2a hybridoma cell strain, staphylococcus aureus PBP2a protein is extracted as an antigen, after the high-purity antigen is obtained, a good immunoreaction is aroused in a mouse body, then a hybridoma technology is adopted, two cell strains with high affinity and specificity are screened, and the corresponding antibody is prepared; the two antibodies are used as a gold-labeled antibody and a coating antibody in a colloidal gold immunoassay kit, and the kit can be used for detecting PBP2a protein in methicillin-resistant staphylococcus aureus and can be used for developing related in-vitro diagnostic reagents.

The invention discloses a porcine muscle stem cell line capable of being cultured in vitro for a long time and application of the porcine muscle stem cell line, and belongs to the technical field of stem cells and cell culture meat. The porcine muscle stem cell line is prepared by the following steps: performing CDKN2A gene knockout on porcine muscle stem cells by using a CRISPR / Cas9 gene editing technology, performing monoclonal screening and genotype identification to obtain a cell strain with successful CDKN2A knockout, and then performing carrier-free and serum-free suspension culture domestication on the cell strain. And a carrier-free and serum-free porcine muscle stem cell line capable of being subjected to in-vitro long-term suspension culture is obtained. Verification shows that the CDKN2A knockout porcine muscle stem cell line subjected to carrier-free and serum-free suspension culture domestication not only can be subjected to carrier-free and serum-free suspension culture in a shake flask for a long time, but also can maintain a good differentiation characteristic. The porcine muscle stem cell line which can be cultured on a large scale, is derived from edible animals and can be cultured in vitro for a long time is created, and excellent cell resources are provided for commercial production of cultured meat.

The invention discloses a site for stably expressing protein in a CHO cell gene NW023276806.1 and application of the site, and belongs to the technical field of biological genes. The site belongs to a fixed position in a CHO cell genome, different protein genes are introduced based on a micro-homologous end connection mechanism through a CRISPR / Cas9 tool, and stable expression is carried out. By adopting a site-specific integration method, a target gene is integrated to a stable expression area in a site-specific manner, repeated high-expression monoclonal screening is effectively avoided, and an MMEJ mechanism is introduced to integrate a donor fragment, so that the research and development time for constructing a stable expression cell strain in biological pharmacy can be effectively shortened, and the cost is reduced.

The invention belongs to biotechnology, and particularly relates to an African swine fever virus p30 protein monoclonal antibody as well as a preparation method and application thereof. The main technology comprises the following steps: immunizing a BALB / c mouse by using a recombinant p30 protein prepared by prokaryotic expression as an antigen, and carrying out cell fusion, indirect ELISA method screening, limited dilution method subcloning and hybridoma continuous subculture by using a hybridoma technology to obtain a hybridoma cell line capable of stably secreting an anti-African swine fever virus p30 protein monoclonal antibody. The invention also discloses a sequence of an immunogen of the prepared monoclonal antibody, a subtype of the monoclonal antibody, a CDR sequence, a preservation number of a hybridoma cell strain and application of the monoclonal antibody in a Western Blot experiment, an indirect immunofluorescence experiment, a neutralization experiment and a blocking ELISA method for detecting the African swine fever virus antibody in swine serum.

The invention relates to the technical field of biomedicine, in particular to an anti-influenza A virus NP protein antibody pair and application thereof. The antibody pair comprises two monoclonal antibodies; the monoclonal antibody comprises a heavy chain variable region and a light chain variable region; one monoclonal antibody in the monoclonal antibody pair is named as a 10G10 antibody, and the other monoclonal antibody is named as a 11F8 antibody. The invention also discloses a nucleic acid molecule for coding the monoclonal antibody and a hybridoma cell strain for secreting the monoclonal antibody. In addition, the invention also discloses a kit for detecting influenza virus protein, which comprises the monoclonal antibody pair, the 10G10 antibody is used as a capture antibody, and the 11F8 antibody is used as a labeled antibody. The antibody for resisting the influenza A virus NP protein provided by the invention has relatively good specificity on the influenza virus protein.

The invention belongs to the field of monoclonal antibodies, and particularly relates to a mouse anti-pelteobagrus fulvidraco IgM monoclonal antibody, a hybridoma cell strain secreting the monoclonal antibody and application, a CDR region of a heavy chain variable region of the mouse anti-pelteobagrus fulvidraco IgM monoclonal antibody comprises three sequences shown in SEQ ID NO.1-3, and a CDR region of a light chain variable region of the mouse anti-pelteobagrus fulvidraco IgM monoclonal antibody comprises three sequences shown in SEQ ID NO.4-6; the mouse anti-pelteobagrus fulvidraco IgM monoclonal antibody can be obtained by being secreted by a hybridoma cell strain PFM-1B7C12A1B2 with the preservation number of CCTCC (China Center for Type Culture Collection) NO: C2024234. The mouse anti-pelteobagrus fulvidraco IgM monoclonal antibody provided by the invention can be used for Western blot and ELISA specific detection of IgM in a pelteobagrus fulvidraco secretion form, and can be used for flow cytometry identification of pelteobagrus fulvidraco IgM + B cells. The monoclonal antibody is good in specificity, high in sensitivity and strong in affinity, can be used for structural analysis, immune response level detection and the like of pelteobagrus fulvidraco IgM, and lays a foundation for in-depth study of a pelteobagrus fulvidraco immune system and establishment of a vaccine immune effect evaluation method taking the antibody level as an index.

The invention provides an FWCH2 monoclonal antibody and a preparation method and application of a kit of the FWCH2 monoclonal antibody. A monoclonal antibody hybridoma cell strain FWCH2 is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.46112, and the prepared monoclonal antibody can effectively recognize the FWCH2.

The invention discloses a construction method and application of a gene modified mesenchymal stem cell. The construction method comprises the following steps: constructing a recombinant lentivirus carrying PD-L1 and ICAM1 genes; the recombinant lentivirus carrying the PD-L1 gene and the ICAM1 gene is transfected with mesenchymal stem cells, and the mesenchymal stem cells are screened by puromycin, so that an engineered mesenchymal stem cell strain modified by the PD-L1 gene and the ICAM1 gene is obtained; the mesenchymal stem cells provided by the invention are modified by engineering genes of PD-L1 and ICAM1, and are screened by puromycin to obtain a mesenchymal stem cell strain capable of stably and highly expressing the genes of PD-L1 and ICAM1; after the mesenchymal stem cells highly expressing the PD-L1 and ICAM1 genes are intravenously injected into an autoimmune hepatitis animal model, the autoimmune hepatitis symptom can be remarkably relieved, the liver injury degree can be reduced, the expression of inflammatory factors can be regulated, the inflammatory reaction can be inhibited, and the obvious curative effect can be achieved.

The invention discloses a bovine rotavirus monoclonal antibody and a preparation method of a bovine rotavirus quantum dot immunochromatography test strip, and belongs to the technical field of bovine rotavirus monoclonal antibodies. According to the invention, a hybridoma cell strain BRV 3G9 is utilized, and a monoclonal antibody is prepared by adopting an ascites method; the BRV 3G9 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.46138. The BRV 3G9 is named as BRV 3G9. According to the present invention, the concentrated live virus immunized mouse is adopted to prepare the monoclonal antibody with strong immunoreaction, and the BRV quantum dot immunochromatography test strip is researched and developed on the basis of the monoclonal antibody, and has advantages of convenient use, high efficiency, high sensitivity, strong specificity and the like, and can achieve the rapid clinical differential diagnosis of calf diarrhea pathogens, the problem that grassroots veterinarians lack a clinical rapid detection technology is solved, the diagnosis and treatment level and the prevention and treatment effect are improved, and technical guarantee is provided for efficiently preventing and treating calf diarrhea and guaranteeing calf health.

The invention provides a monoclonal antibody capable of recognizing a recombinant EGF-CRM197 vaccine and a preparation method thereof, on the basis of researching, developing and preparing the recombinant EGF-CRM197 vaccine, after the recombinant EGF-CRM197 vaccine is further immunized and inoculated to a mouse, mouse spleen cells and mouse myeloma cells are fused, and the monoclonal antibody capable of recognizing the recombinant EGF-CRM197 vaccine is obtained. And screening a hybridoma cell strain which generates a specific monoclonal antibody for recognizing the recombinant EGF-CRM197 vaccine, and sequencing to obtain an antibody sequence. After the hybridoma cells are cultured in a mouse body, the monoclonal antibody for recognizing the recombinant EGF-CRM197 vaccine can be prepared in vitro through methods of chromatographic purification and the like. The method can be used for detecting the release titer of the recombinant EGF-CRM197 vaccine and evaluating the immune efficacy after the recombinant EGF-CRM197 vaccine is immunized.

The invention discloses a hybridoma cell strain, a monoclonal antibody, a linear epitope antigen and application thereof, and relates to the field of genetic engineering, in particular to a hybridoma cell strain, a monoclonal antibody, a linear epitope antigen and application thereof. The preservation number of the hybridoma cell strain P27-A1 is CGMCC (China General Microbiological Culture Collection Center) No. 46352. The hybridoma cell strain P27-A1 is prepared from feline leukemia virus p27, and a monoclonal antibody secreted by the hybridoma cell strain P27-A1 recognizes a linear epitope antigen of feline leukemia virus p27 protein; the kit for the feline leukemia virus p27 comprises the monoclonal antibody A1 secreted by the hybridoma cell strain P27-A1. The monoclonal antibody secreted by the hybridoma cell strain can greatly improve the sensitivity of the p27 protein for detecting feline leukemia virus. The invention provides the monoclonal antibody secreted by the hybridoma cell strain for identification, and the monoclonal antibody can be used for preparing a diagnostic kit such as a colloidal gold test strip.

The invention belongs to the field of veterinary biotechnology detection, and particularly relates to a hybridoma cell strain and a monoclonal antibody for detecting or identifying MDV-1 and application of the hybridoma cell strain and the monoclonal antibody. The hybridoma cell strain MDV-1Mab-pp38-E2 is obtained through screening by taking pp38 protein of MDV-1 as immunogen, the preservation number is CCTCC NO: C202501, the monoclonal antibody generated by the hybridoma cell strain can recognize I-type chicken Marek's disease virus strains with different serum, and the monoclonal antibody can be used for identifying the type I chicken Marek's disease virus strains with different serum types. The kit has good specificity and sensitivity and can be used for exogenous virus detection of the serum type I chicken Marek's disease virus in poultry virus live vaccines without cross reaction with serum type II chicken Marek's disease virus, serum type III chicken Marek's disease virus, chicken egg drop syndrome virus (EDSV) and other common poultry disease viruses, and the kit has good specificity and sensitivity and can be applied to exogenous virus detection of the serum type I chicken Marek's disease virus in poultry virus live vaccines. The kit can also be used for clinical identification, virus content determination and epidemiological investigation of serotype I chicken Marek's disease virus.

The invention relates to a CHO-S cell strain capable of stably expressing H5N1 hemagglutinin protein and a construction method of the CHO-S cell strain, and belongs to the field of bioengineering.H5N1 hemagglutinin protein expression plasmids are obtained in an in-vitro synthesis and seamless cloning mode, 293T cells are transfected through the H5N1 hemagglutinin protein expression plasmids, and it is proved that the H5N1 hemagglutinin protein is expressed; the CHO-S cell strain capable of stably expressing the H5N1 hemagglutinin protein is obtained by transfecting CHO-S cells by using the H5N1 hemagglutinin protein expression plasmids and carrying out multiple rounds of cloning and screening, so that a basis is provided for obtaining H5N1 recombinant protein influenza vaccines.

The invention provides a K1143 monoclonal antibody and a preparation method and application of a kit of the K1143 monoclonal antibody. The monoclonal antibody hybridoma cell strain K1143 is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.46110, and the prepared monoclonal antibody can effectively recognize K1143. The invention further discloses a preparation method of the monoclonal antibody K1143.

The invention provides a ZN428 monoclonal antibody and a preparation method and application of a kit of the ZN428 monoclonal antibody. The monoclonal antibody hybridoma cell strain ZN428 is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.46111, and the prepared monoclonal antibody can effectively recognize the ZN428.

The invention relates to the technical field of biological medicine, in particular to a monoclonal antibody of BRVA virus VP7 protein and an epitope and application of the monoclonal antibody. The invention provides a hybridoma cell strain (preservation number: CCTCC NO: C202570). A monoclonal antibody prepared from the hybridoma cell strain can be combined with VP7 protein of G6, G8 and G10 type BRVA; the antigen epitope amino acid sequence of the monoclonal antibody is SEQ ID NO: 1. The monoclonal antibody and the antigen epitope peptide provided by the invention have broad-spectrum neutralizing activity, and provide a new strategy and tool for prevention and control of BRVA.

The invention provides an LRC58 monoclonal antibody and a preparation method and application of a kit of the LRC58 monoclonal antibody. The monoclonal antibody hybridoma cell strain LRC58 is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.46113, and the prepared monoclonal antibody can effectively recognize the LRC58. The invention further discloses a method for preparing the monoclonal antibody.

The invention provides an SCGB1D4 monoclonal antibody and a preparation method and application of a kit of the SCGB1D4 monoclonal antibody. An applicant is preserved in the China General Microbiological Culture Collection Center (CGMCC) through a hybridoma cell strain SCGB1D4, and the preservation number is CGMCC No.45958. The prepared monoclonal antibody can effectively recognize the SCGB1D4.

The invention is applicable to the technical field of C-reactive protein detection, and provides an anti-C-reactive protein monoclonal antibody idiotype antibody, a preparation method and application, and the anti-C-reactive protein monoclonal antibody idiotype antibody is secreted by a hybridoma cell strain; the hybridoma cell strain is a 7C4 hybridoma cell strain; the preparation method of the anti-C-reactive protein monoclonal antibody idiotype antibody comprises the following steps: S1, obtaining hybridoma cells: preparing an anti-C-reactive protein monoclonal antibody Fab fragment, then carrying out animal immunization, then carrying out cell fusion, and finally screening and cloning the hybridoma cells; and S2, preparing and screening an anti-C reactive protein monoclonal antibody idiotype antibody. The anti-C-reactive-protein monoclonal antibody idiotype antibody is used for replacing C-reactive protein to serve as a labeled antibody, the labeling process is simple, the labeling efficiency is high, and meanwhile the situation that a final product is affected due to instability of the C-reactive protein is avoided. Experimental results show that the detection method is good in specificity and wide in linear range.

The invention discloses an anti-HCV (hepatitis C virus) core antigen monoclonal antibody, a hybridoma cell strain and application, and particularly relates to the hybridoma cell strain screened according to a common conserved sequence of core proteins of different genotypes of HCV, the antibody secreted by the hybridoma cell strain is applied to HCV antigen detection, different genotypes of hepatitis C viruses can be detected, and the monoclonal antibody has good inclusiveness and can be applied to detection of HCV antigens. The technical effect can be realized by only two antibodies, so that the production cost of the HCV antigen detection kit can be reduced, the production difficulty can be reduced, and the production efficiency can be improved.

The invention discloses a breast adenoma B3 type source cell strain as well as construction and application thereof, the breast adenoma B3 type source cell strain is named as human breast adenoma cell Hthy2301 Homo sapiens and is preserved in China Center for Type Culture Collection on March 14, 2024, and the preservation number is CCTCC NO: C202478. The human thymoma cell line is stable in character, can be passaged in vitro for a long time, and has high proliferation activity. The human thymoma cell line disclosed by the invention can be used as an effective cell model, and a new experimental model can be provided for deeply researching the occurrence, development and transfer mechanisms of thymoma, the drug resistance mechanism, drug screening, drug effect evaluation and the like.

The invention discloses a PROTAC compound for targeted degradation of ALK protein and application of the PROTAC compound, the structural formula of the compound is # imgabs0 #, and Linker is a linker. The PROTAC compound capable of degrading the ALK protein in a targeted manner is applied to preparation of drugs for treating ALK positive tumors. The compound can effectively solve the problem that an existing small-molecule inhibitor cannot degrade ALK protein, and the compound can inhibit proliferation of an ALK drug-resistant mutation cell strain.

The invention provides a subunit fusion protein RBD-Fc on the surface of a porcine D-type coronavirus and a preparation method and application thereof, the preparation method comprises the following steps: 1) cloning a gene sequence as shown in SEQ ID NO.5 into a eukaryotic expression vector to obtain a recombinant plasmid containing a fusion protein RBD-Fc coding gene; (2) transfecting a recombinant plasmid containing the fusion protein RBD-Fc coding gene into an expression cell; 3) culturing, screening and domesticating the expression cells in the step 2) to obtain a highly expressed cell strain; and 4) fermenting and culturing the cell strain in the step 3), and purifying to obtain the subunit fusion protein RBD-Fc. According to the present invention, the RBD-Fc can be subjected to mass soluble expression, the protein is stable, the problems that the porcine D-coronavirus surface S protein cannot be subjected to large-scale expression and the like in the prior art are solved, the preparation method is simple, and the cost is low.

The invention provides application of a compound for regulating and controlling an METTL3 target spot in preparation of an anti-enterovirus drug, and belongs to the field of biological medicine, the compound is AN-465 / 42162872, and the compound has a relatively strong anti-EV71 effect in both in-vitro and in-vivo infection models. An in-vitro cell experiment, EV71 infected C57BL / 6 suckling mouse model, m6A methylation sequencing and other methods are utilized to research the activity and potential action mechanism of anti-EV71, and the result shows that AN-465 / 42162872 can inhibit duplication of EV71 in a human RD cell strain in a dose-dependent manner under the condition of maintaining relatively good cell activity, so that the application of AN-465 / 42162872 in the preparation of the EV71 drug is developed. The action mechanism is mainly as follows: the methylation level of the 5002 adenine in the 2C region of EV71 nucleic acid is remarkably reduced by regulating the METTL3, so that virus replication is inhibited, and the METTL3 has relatively high anti-EV71 drug utilization value.

The invention provides application of a phenolic compound and / or pharmaceutically acceptable salt thereof in preparation of a medicine for treating breast cancer and a pharmaceutical composition, and relates to the technical field of biological medicines. The invention discloses application of phenolic compounds and / or pharmaceutically acceptable salts thereof in preparation of medicines for treating breast cancer. The phenolic compounds comprise a compound 1 and / or a compound 2. The compound 1 (arnebidin) and the compound 2 ((+)-(R)-de-O-metalasiodiplodin) adopted in the invention have good inhibition effects on human triple-negative breast cancer MDA-MB-231 cell strains, the IC50 value of the compound 1 is 1.16 [mu] M, and the IC50 value of the compound 2 is 0.68 [mu] M, so that a new choice is provided for developing drugs for treating triple-negative breast cancer, and the compound 1 and the compound 2 have good application prospects. The compound has a good application prospect in preparation of medicines for treating breast cancer, especially medicines for treating triple-negative breast cancer.

The invention discloses a monoclonal antibody targeting all CD45 subtypes and application of the monoclonal antibody, and belongs to the field of monoclonal antibody preparation. The hybridoma cell strain capable of stably secreting all anti-human CD45 subtype antibodies is successfully obtained by stably expressing short and long molecular subtypes of human CD45 extracellular regions in L929 mouse fibroblasts, immunizing BALB / c mice with L929 cells expressing the two molecular subtypes of human CD45, and fusing splenocytes with SP20 myeloma cells after the serum titer reaches the standard. And the monoclonal antibody targeting all subtypes of CD45 is obtained. The monoclonal antibody provided by the invention shows a high-affinity binding characteristic with all human CD45 molecular subtypes, has important application value in the aspect of human CD45 molecular detection, and can be used as a therapeutic antibody in the fields of tumor immunotherapy, autoimmune disease treatment, transplant rejection resistance and the like.

The invention discloses a cell strain capable of generating antibodies for detecting Pseudomonas amygdali and diseases caused by the Pseudomonas amygdali and application of the cell strain. The preservation number of the cell strain is CCTCC (China Center For Type Culture Collection) NO: C2024169 and / or CCTCC NO: C2024170. The monoclonal antibody generated by the monoclonal antibody hybridoma cell strain can be prepared into handheld colloidal gold rapid test paper, and the pseudomonas amygdali in a sample can be rapidly and sensitively detected on site.

The invention discloses a recombinant protein and a detection reagent for detecting encephalitis of an anti-GABABR antibody, and belongs to the technical field of neuroimmunology diagnos.A GABABR1 partial sequence, a GABABR2 partial sequence and a transmembrane sequence (and a fluorescent label) are adopted to construct a cell strain for stably and highly expressing the recombinant protein by transfecting CHO cells or HEK293 cells, and the recombinant protein and the detection reagent are used for detecting the encephalitis of the anti-GABABR antibody. The sensitivity and the specificity of the kit in anti-GABABR antibody encephalitis detection can be remarkably improved, the aim of detecting the antibody by using a fixed CBA method or a living cell CBA method can be achieved, and the kit is suitable for auxiliary diagnosis of autoimmune encephalitis of cerebrospinal fluid / serum.

The invention provides an R3HD4 monoclonal antibody and a preparation method and application of a kit of the R3HD4 monoclonal antibody. The monoclonal antibody hybridoma cell strain R3HD4 is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.46114, and the prepared monoclonal antibody can effectively recognize R3HD4. The invention further discloses a preparation method of the monoclonal antibody.

The invention provides a CK052 monoclonal antibody and a preparation method and application of a kit of the CK052 monoclonal antibody. The monoclonal antibody hybridoma cell strain CK052 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.46107. The prepared monoclonal antibody and the kit thereof can effectively recognize the CK052.