1748 results about "Cell activity" patented technology

The present invention generally provides methods for B-cell reduction in an individual using CD37-specific binding molecules. In particular, the invention provides methods for B-cell reduction using CD37-specific binding molecules alone, or a combination of CD37-specific binding molecules and CD20-specific binding molecules, in some instances a synergistic combination. The invention further provides materials and methods for treatment of diseases involving aberrant B-cell activity. In addition, the invention provides humanized CD37-specific binding molecules.

RNA prepared by in vitro transcription using a polymerase chain reaction (PCR)-generated template can be introduced into a cell to modulate cell activity. This method is useful in de-differentiating somatic cells to pluripotent, multipotent, or unipotent cells; re-differentiating stem cells into differentiated cells; or reprogramming of somatic cells to modulate cell activities such as metabolism. Cells can also be transfected with inhibitory RNAs, such as small interfering RNA (siRNA) or micro RNA (miRNA), or combinations thereof to induce reprogramming of somatic cells. For example, target cells are isolated from a donor, contacted with one or more RNA's causing the cells to be de-differentiated, re-differentiated, or reprogrammed in vitro, and administered to a patient in need thereof. The resulting cells are useful for treating one or more symptoms of a variety of diseases and disorders, for organ regeneration, and for restoration of the immune system.

The invention, in some aspects relates to compositions and methods for altering cell activity and function and the use of light-activated ion pumps (LAIPs).

The invention is directed to methods of modulating an immune response in an animal, comprising administering a composition comprising one or more soluble CD1d complexes, in particular non-specific soluble CD1d complexes. Soluble CD1d complexes comprise a soluble CD1d polypeptide, a β2-microglobulin polypeptide, and a ceramide-like glycolipid antigen bound to the CD1d antigen binding groove, and in certain embodiments, an immunogen. The administration of compositions of the present invention affects the activity of CD1d-restricted NKT cells, and in particular, allows for multiple administrations without causing CD1d-restricted NKT cell anergy.

Methods for modulating (inhibiting or stimulating) retinoid-related orphan receptor γ (RORγ) activity. This modulation has numerous effects, including inhibition of T_H-17 cell function and/or T_H-17 cell activity, and inhibition of re-stimulation of T_H-17 cells, which are beneficial to treatment of inflammation and autoimmune disorders. Stimulation of RORγ results in stimulation of T_H-17 cell function and/or activity which is beneficial for immune-enhancing compositions (e.g., vaccines).

The present invention provides reagents and methods for modulating cone photoreceptor activity, and devices for assessment of cone photoreceptor activity.

The invention provides a serum-free cell culture fluid suitable for enriching and culturing tumour stem cells. DMEM/f12 serves as the basic culture fluid of the serum-free cell culture fluid and transferrin, insulin and other substances are also added as the components of the serum-free cell culture fluid. The serum-free cell culture fluid provided by the invention can efficiently enrich tumour stem cells from malignant tumour cell strains and tumour tissues. In addition, the serum-free cell culture fluid can promote stable growth of the tumour stem cells and maintain fine cell activity and physiological properties of the tumour stem cells, thus being very suitable for research fields related to tumour cells and tumour stem cells.

Extracts from plant material, or semi-purified/purified molecules or compounds prepared from the extracts that demonstrate the ability to modulate one or more cellular activities are provided. The extracts are capable of slowing down, inhibiting or preventing cell migration, for example, the migration of endothelial cells or neoplastic cells and thus, the use of the extracts to slow down, inhibit or prevent abnormal cell migration in an animal is also provided. Methods of selecting and preparing the plant extracts and methods of screening the extracts to determine their ability to modulate one or more cellular activity are described. The purification or semi-purification of one or more molecules from the described extracts is also contemplated as well as the use of these molecules, alone or in combination with an extract, to slow down, inhibit or prevent abnormal cell migration in an animal.

The invention relates to a novel bufadienolides compound and a preparation method and application thereof, and provides a bufadienolides compound having a general formula (I) or pharmaceutical acceptable salts as well as bufadienolides extract or medication composition containing the compound. The compound and the extract have effect for inhibiting tumour cell activity and application to preparation of anti-tumour drugs. The invention also provides the preparation method applicable for industrial production of the compound and the extract.

The invention provides a method for storing mesenchymal stem cells and a method for culturing mesenchymal stem cells. The storing method comprises the following steps of: cutting the umbilical cord into blocks, and putting the blocks into a mixed solution of freezing storage solution and base solution in a volume rate of 1:3 for pretreatment; putting the blocks in the mixed solution of the freezing storage solution and the base solution in a volume rate of 1:1 for treatment at the temperature of 0 DEG C; putting the blocks in the freezing storage solution for treatment at the temperature of 0DEG C; and freeze-storing the blocks in liquid nitrogen. The culturing method is that the stored umbilical cord blocks are used for culture. After babies are delivered, the expensive cell culturing operation of the umbilical cords is not required immediately, the umbilical cords can be directly frozen at the deep low temperature, and the cell activity is not affected. One freezing storage tube can be picked up at any time, the umbilical cords are recovered and successfully cultured and amplified to obtain MSCs, so that the culture operation is not required to be made for each portion of umbilical cord, and the operating cost of the umbilical cord MSCs storage warehouse is greatly lowered.

The invention discloses a mesenchymal stem cell self-preserving liquid which is prepared from the following raw materials according to volume ratios: 1-6% of self platelet lysate, 95-97% of solution medium and 0-1% of vitamin C injection, wherein the solution medium is a mixed sugar electrolyte injection or physiological saline injection. The mesenchymal stem cell self-preserving liquid has the advantages that: growth factors contained in the mesenchymal stem cell self-preserving liquid can maintain the activity state of cells, the vitamin C can maintain the activity of various peroxidases, at the same time, the mixed sugar electrolyte injection is especially suitable for cell preservation, and osmotic pressure is also favorable for nutrient absorption of the cells so as to carry out metabolism; the activity of the mesenchymal stem cells which are preserved in the mesenchymal stem cell self-preserving liquid within 8 hours is still larger than 90%, the cell activity change is not larger than 7%, and the activity of the mesenchymal stem cells which are preserved in the mesenchymal stem cell self-preserving liquid within 12 hours is still maintained at 85%; and by using the mesenchymal stem cell self-preserving liquid, the requirements for human intravenous back-transfusion safety can be met, and the cell preserving problem of long-time transport is solved, thus more patients have opportunity to undergo mesenchymal stem cell treatment.

The present subject matter relates to red emitting mitochondria-targeted aggregation induced emission (AIE) probes as an indicator for membrane potential and mouse sperm activity. The present subjectmatter relates to AIE-active fluorescent probes for reactive oxygen species (ROS) detection and related biological applications, such as inflammation imaging and glucose assay, as well as the preparation and application of red fluorescent AIEgens.

The invention discloses a dendrobium officinale stem cell and an isolated culture method thereof. The method comprises the following steps: culturing plant root tissue containing a quiescent centre, performing subcultring and scale-up culture on quiescent centre stem cell, and then preparing stem cell dry powder or extracting solution according to requirements. The dendrobium officinale stem cell overcomes the problem that dendrobium officinale in vitro material is easy to brown, the cell of a stem cell line is not differentiated, the cell increment is large in a nutritional condition, the culture time is short, and the cell activity is high. The dendrobium officinale stem cell and the method have the advantages that the technical process is easy to operate, the quantity of technological processes is less, the output is high, and the dendrobium officinale stem cell obtained is anti-aging and oxidation resistant, promotes hair growth and high baldness-resistant activity, and can be manufactured into functional health care products and cosmetics for preventing and curing various senescence and baldness and relevant Chinese drugs for preventing senescence and baldness.

A method of making a three-dimensional biocompatible scaffold capable of supporting cell activities such as growth and differentiation, the method includes providing a supporting grid that forms an open network and provides mechanical support of a second biocompatible material. The second biocompatible material has interconnected cavities that allow nutrients, metabolites and soluble factors to diffuse throughout the scaffold. The scaffold design can be understood as a hierarchically organised structure. At the micron to submicron length scale a top/down manufacturing approach is used to make a structure that will constitute the frame into which a bottom/up processing approach is applied to form an open porous scaffold with specific nano sized features. The advantage of this hierarchically organised design is that benefits can be drawn independently from both the micron and the nano sized structures, promoting specific cell activities and providing sufficient mechanical compliance.

The invention discloses an anti-allergic skin-whitening mask solution and a preparation method thereof. The anti-allergic skin-whitening mask solution is prepared from the following components in percentage by weight: 0.1-2 percent of O/W-type emulsifier, 0.1-2 percent of co-emulsifier, 0.1-1 percent of antioxidant, 3-8 percent of emollient, 0.01-0.2 percent of ethylene diamine tetraacetic acid disodium (EDTA-2Na), 0.01-5 percent of desensitizer, 1-8 percent of first moisturizer, 0.01-0.5 percent of thickener, 0.01-0.5 percent of second moisturizer, 0.01-0.5 percent of pH modifier, 0.01-0.5 percent of preservative, 1-6 percent of glucan, 0.01-0.5 percent of rose essential oil, 0.01-0.3 percent of agave leaf extract, 0.5-2 percent of rice bran yeast and the balance of deionized water. The mask solution has the advantages that the preparation method is simple; by virtue of an oil-in-water emulsification system, functional matters in the mask solution are more easily absorbed and permeate into the skin, high activity is kept, the cell activity is comprehensively improved, the skin reviving process is accelerated, the skin is kept wetted and translucent, the health and the elasticity of the skin are restored, and the soothing effect and the repairing effect are achieved.

This invention provides a non-radioactive assay to monitor and quantify the target-cell killing activities mediated by cytotoxic T lymphocytes (CTLs). This assay is predicated on the discovery that apoptosis pathway activation and, in particular, granzyme B activity, provides a measure of cytotoxic effector cell activity. In one embodiment, measurement of CTL-induced granzyme B activation in target cells is achieved through detection of the specific cleavage of fluorogenic granzyme B substrates. This assay reliably detects antigen-specific CTL killing of target cells, and provides a more sensitive, more informative and safer alternative to the standard ⁵¹Cr-release assay most often used to quantify CTL responses. The assay can be used to study CTL-mediated killing of primary host target cells of different cell lineages, and enables the study of antigen-specific cellular immune responses in real time at the single-cell level. As such, the assay can provide a valuable tool for studies of infectious disease pathogenesis and development of new vaccines and immunotherapies.

NTNRalpha, NTNRalpha extracellular domain (ECD), NTNRalpha variants, chimeric NTNRalpha (e.g., NTNRalpha immunoadhesion), and antibodies which bind thereto (including agonist and neutralizing antibodies) are disclosed. Various uses for these molecules are described, including methods to modulate cell activity and survival by response to NTNRalpha-ligands, for example NTN, by providing NTNRalpha to the cell.

The invention relates to an improved mesenchyme stem cell protection solution as well as application and a preparation method thereof. The protection solution can effectively prolong the activity remaining time of mesenchyme stem cells, reduces preparation cost, and has the advantages of wide raw material source, simple preparation, safe and reliable direct clinical application; and after the mesenchyme stem cells are preserved for 48 hours in the protection solution, the cell activity is still above 90 percent, the cell morphology is normal, and the multiplication capacity and mesenchyme stem cell phenotype characteristics are not influenced.

The invention discloses a novel chondrocyte epimatrix membrane and a preparation method thereof. The membrane contains cell epimatrix ingredients which have bioactivity and cell activity, wherein the cell epimatrix comprises the following main ingredients in percentage by weight: 80 to 95 percent of bionic type II collagen in an acellular cartilage matrix and 10 to 20 percent of hyaluronic acid, 10 to 20 percent of aminopolysaccharide and the like which serve as a chondrocyte epimatrix; the chondrocyte epimatrix biological membrane prepared from the acellular cartilage matrix has the bionic chondrocyte epimatrix ingredients, is excellent in cell consistency, does not arouse immunological rejection of host cartilage tissue, can be used for repairing the damage of hyaline cartilage tissue of joints and reconstructing the hyaline cartilage tissue, and also can be used for a seed cell inoculating vector and a growth factor sustained-release vector; and the chondrocyte epimatrix contains blood vessel inhibiting factor ingredients, so the membrane also can be used for preventing the conglutination of tissue such as muscle tendons.

The invention discloses an adherent-cultured cell activity detection method and device based on impedance spectroscopy. An interdigital electrode, which is parallel with the bottom surface of a culture dish, is arranged at the bottom of the interior of the culture dish; two poles of the interdigital electrode are connected to a controller by virtue of lead wires; a DDS (direct digital synthesizer) frequency generator, which is controlled by a single chip microcomputer in the controller, is promoted to transmit sine waves which are in n pieces of frequencies from high frequency to low frequency; a current excitation signal, after being transformed by a voltage-controlled current source, is outputted to the interdigital electrode; n pieces of response voltages of the interdigital electrode, by virtue of a differential amplifier, are inputted to a single chip microcomputer after undergoing analog-to-digital conversion; the single chip microcomputer is used for calculating a complex impedance value under different frequencies, and is used for calculating a dual-layer capacitance value in accordance with an equivalent circuit model and the complex impedance value; the capacitance value of a dual-layer capacitor in an equivalent circuit serves an index for assessing cell activity, so that more accurate cell activity information can be obtained; and the method and the device can be used for monitoring the cell activity in real time, cannot damage the steady state of cells and can achieve quantitative measurement on the cell activity.