589 results about "Cell activity" patented technology

The invention relates to an organ-like core-shell microsphere as well as a preparation method and application thereof. The preparation method comprises the steps that 1, raw materials are prepared, specifically, shell raw materials and inner core raw materials are prepared, the shell raw materials comprise a photoinitiator and methacrylated hyaluronic acid (HAMA), and the inner core raw materials comprise organoid precursor cell suspension and matrigel; 2) molding: extruding the dispersion phase of the shell raw material wrapping the core raw material to a continuous phase by using a micro-fluidic chip and a high-precision injection pump, cutting the dispersion phase into liquid drops by the continuous phase, and performing illumination curing on the liquid drops to form core-shell microspheres; and (3) incubating, namely culturing the core-shell microspheres in a culture medium, so that the organoid precursor cells in the inner core are developed into organoids. According to the preparation method, rapid forming and curing of the organ-like precursor cells carried by the matrigel are achieved, so that the time of the organ-like precursor cells staying in the oil phase is shortened, the cells can obtain oxygen and nutrient substances, and the cell activity is improved.

The invention discloses preparation of a whey protein peptide mixture with immune and muscle-building effects, and relates to the technical field of food processing and bioengineering. The neutral protease is adopted and the pH is adjusted, so that the enzymolysis efficiency is effectively improved, and the enzymolysis time is greatly shortened; meanwhile, the whey protein peptide which is uniform in molecular weight distribution, free of peculiar smell and good in thermal stability is successfully prepared by accurately controlling enzymolysis conditions and combining microbial fermentation. The protein peptide can effectively improve thymus indexes and spleen indexes, enhance the proliferation capacity of splenic lymphocytes and the activity of NK cells, play an excellent immune regulation role and have a remarkable muscle building effect. The preparation process has the advantages of high efficiency, low cost, simplicity, convenience and the like, gives consideration to high quality and high functionality of the product, and has great application value.

The invention provides a uterine muscle cell tension analysis method based on a uterine muscle cell activity image, and relates to the technical field of image processing. The method comprises the steps of tension analysis mode obtaining, resting state analysis mode tension analysis, uterine contraction state analysis mode tension analysis and tension analysis result feedback. According to the method, a tension analysis mode is obtained, if the tension analysis mode is a resting state analysis mode, a resting uterine muscle cell image is obtained, and a resting uterine muscle cell tension analysis result is obtained, and if the tension analysis mode is a uterine contraction state analysis mode, a uterine contraction uterine muscle cell image is obtained, and a uterine contraction uterine muscle cell tension analysis result is obtained. Finally, the uterine muscle cell tension analysis result is transmitted to a preset medical staff terminal, the reliability of the uterine muscle cell tension analysis result is improved, and the problem that in the prior art, the reliability of the uterine muscle cell tension analysis result is unstable due to the difference of ultrasonic probe scanning is solved.

The invention relates to the field of image processors and biological medicines, and discloses an NK cell activity rapid detection method based on image processing. The method comprises the following steps: acquiring an unmarked time sequence phase image sequence of an NK cell and target cell co-culture system; performing cell instance segmentation to track individual cells; extracting a morphological dynamic characteristic parameter set of the target cell, wherein the morphological dynamic characteristic parameter set comprises a volume change rate, a phase gradient entropy, a cytoplasm phase fluctuation frequency and a nuclear region phase mean value; inputting the parameters into a pre-trained death state discrimination model, and outputting a death probability; and calculating a killing efficiency index based on the death probability evolution curve, and judging the activity level of the NK cells. The system comprises a phase image acquisition unit, a cell segmentation unit, a feature extraction unit, a death judgment unit and an activity judgment unit. Through unmarked imaging and deep learning fusion analysis, high-precision, real-time, quantitative and ultra-early NK cell activity evaluation is realized, and the method is suitable for clinical instant inspection and immunotherapy monitoring.

The invention relates to the technical field of biomedical engineering and microfluidics, in particular to a fluid dynamics topological optimization design method of a circulating tumor cell sorting structure, which comprises the following steps of: establishing a three-dimensional flow field model containing a key separation area in a preset design domain, dispersing by adopting a tetrahedral mesh and setting boundary conditions; the method comprises the following steps: establishing a multi-objective optimization function based on a maximized transverse focusing distance, a minimum shear force peak value and a total pressure drop, dispersing a design domain into units and endowing the units with density variables, and realizing global automatic topological optimization by adopting penalty material interpolation and minimum flow velocity threshold constraint. An optimization result is subjected to structural reconstruction processing to generate a CAD model adaptive to micro-nano machining, closed-loop feedback is formed through secondary verification and experimental verification of computational fluid mechanics, it is ensured that the design meets the requirements for sorting efficiency and cell activity, limitation of a traditional flow channel form is broken through, and the design freedom degree, manufacturability and application value are improved.

The invention discloses an NKT cell culture optimization method, and belongs to the technical field of cell culture.The method comprises the steps that firstly, PBMC is sorted through a spiral micro-fluidic chip integrated with CD3 / CD56 double-labeled magnetic beads, and CD3 + CD56 + cells with the purity larger than or equal to 95% are obtained through the synergistic effect of dielectrophoretic force and a magnetic field; then, in a GIBCO culture solution added with the IL-2 / IL-15 / alpha-GalCer sustained release microspheres, the pH, dissolved oxygen and lactic acid concentration are accurately regulated and controlled through a real-time monitoring system, and temperature-oxygen partial pressure gradient culture is adopted; and finally, three times of centrifugation are combined with NKT4 solution treatment, and the cells are collected in cooperation with a gradient cooling procedure, so that the cell activity maintenance rate is greater than or equal to 95%. Compared with the traditional technology, the method has the advantages that the NKT cell sorting purity and activity are remarkably improved, the culture environment is regulated and optimized through dynamic metabolism, and accurate control over the culture process is achieved.

The invention discloses a function-enhanced mesenchymal stem cell preparation obtained by utilizing arthritis effusion and hypoxia pretreatment of mesenchymal stem cells as well as a preparation method and application of the function-enhanced mesenchymal stem cell preparation, and relates to the technical field of biological medicines. The preparation method of the function-enhanced mesenchymal stem cell preparation obtained by utilizing arthritis effusion and hypoxia pretreatment of mesenchymal stem cells comprises the following steps: S1, obtaining mesenchymal stem cells and performing hypoxia amplification passage; s2, pretreatment of arthritis effusion; S3, pre-stimulation of the arthritis effusion of the mesenchymal stem cells to obtain function-enhanced mesenchymal stem cells; s4, index detection and screening; s5, preparing a function-enhanced mesenchymal stem cell preparation; optimized low-oxygen pretreatment is adopted, so that the cell activity is improved; the mesenchymal stem cells subjected to joint pretreatment of arthritis effusion and hypoxia enhance expression and secretion of anti-inflammatory factors and immunomodulatory molecules in the cells; the pre-stimulated mesenchymal stem cells show a stronger immunosuppression function in vitro.

The invention discloses a single cell activity evaluation method based on cell nucleus geometric parameters and a Bayesian framework, which comprises the following steps: establishing a HeLa cell activity gradient model through adriamycin induction, carrying out cell nucleus segmentation by adopting Cell pose 3.0 and extracting 26 geometric features, screening a high-confidence sample in combination with a Bayesian deep learning framework, and evaluating the activity of a single cell according to the high-confidence sample. And constructing a multi-modal feature fusion model to integrate geometric features and deep semantic features, and finally realizing unmarked and high-precision single cell activity evaluation. The method overcomes the limitation that a traditional technology depends on biochemical markers, has the advantages of being easy and convenient to operate, high in flux and high in interpretability, and provides an innovative technical means for tumor liquid biopsy and precise medical treatment.

The invention provides a precise magnetic control whole blood rare cell flow magnetic separation system, a magnetic separation and identification method and application, and belongs to the technical field of micro-control flow biological detection. According to the invention, the magnetic separation chip can form a gradient magnetic field, and cells are fixed at a certain position on the sealing film under the combined action of magnetic field force and friction force, so that the separation of the cells is realized. Whole blood cell detection is carried out based on the gradient magnetic field, whole blood sample loading treatment can be directly carried out, the problem that the capture rate is reduced due to mechanical death, damage and stress gene expression caused by repeated elution / transfer steps is avoided, and the capture efficiency and cell activity of the chip are guaranteed; the magnetic field provided by electricity is not needed, the cost is low, the detection accuracy is high, the problems existing in existing CTC detection are solved, economic and practical values are achieved, further development of CTC detection can be promoted, and a novel and effective method is provided for complete blood cell separation detection.

The invention discloses a gradient alcohol extraction preparation method of compound honeysuckle flower drops for improving T cell activity, and relates to the technical field of traditional Chinese medicine compound preparations, honeysuckle flower and radix astragali are selected for pretreatment, an ethanol aqueous solution and an interaction regulating agent are added for infiltration, an online traditional Chinese medicine compound preparation HPLC is adopted for dynamic alcohol concentration adjustment and temperature extraction under monitoring of the traditional Chinese medicine compound preparation, the method disclosed by the invention has the advantages that the interaction regulating agent is added in the raw material infiltration stage to block bad interaction among active components, and the online monitoring system is utilized to dynamically adapt the ethanol concentration and the extraction temperature to realize step-by-step efficient dissolution of the multi-polar components; the antioxidant is matched to inhibit oxidation of thermosensitive components, and the solubilizer assists dissolution of insoluble components, so that the core defect that synergistic extraction of multi-polar active components, degradation of the thermosensitive components and interaction inhibition of the components in the compound honeysuckle flowers cannot be considered in the prior art is overcome, and loss or antagonism of the active components is effectively avoided.

The invention discloses a multifunctional hydrogel patch and a preparation method and application thereof.The biological patch comprises decellularized freeze-dried tumor tissue (dFCT) and a hydrogel layer loaded with hair melanin nanoparticles (HNPs), the dFCT is obtained by decellularizing and freeze-drying the tumor tissue, and rich natural extracellular matrix components are reserved; hNPs is extracted from hair and has excellent active oxygen scavenging capacity and photothermal conversion performance; it is preferable that the hydrogel layer is a methacrylated gelatin (GelMA). The patch disclosed by the invention shows a remarkable cell viability promoting effect and antibacterial ability in vitro, and can accelerate wound healing by reducing oxidative stress, inhibiting inflammatory response and promoting angiogenesis in vivo. The patch is simple and convenient in preparation process and natural in component source, has multiple functions of resisting bacteria, resisting oxidation, promoting tissue regeneration and the like, and is suitable for wound repair treatment.

The invention discloses a pseudo-ginseng exosome-like nano vesicle loaded with an epidermal growth factor / application of the pseudo-ginseng exosome-like nano vesicle in wound healing. The research shows that not all exosome-like nano-vesicles from Chinese herbal medicines capable of promoting wound healing have the effect of promoting wound healing, the action mechanism of the pseudo-ginseng exosome-like nano-vesicles in wound healing is further explored, and the research shows that PNVs can promote the proliferation and migration of an epidermal keratinocyte line, so that the PNVs can be used for promoting the healing of the wound. The treatment can be realized by regulating and controlling the activity of immune cells to promote cell proliferation and migration, and the wound healing effect of the EGF-loaded EGF-coated PNVs is remarkably enhanced, so that the EGF-loaded EGF-coated PNVs have a synergistic effect. The invention not only provides a theoretical basis for deeply researching PELNVs, but also provides a new method and a new idea for treating acute wounds.

The invention provides a nasal delivery preparation for cerebral diseases as well as a preparation method and application of the nasal delivery preparation, and relates to the technical field of pharmaceutical preparations. The nasal delivery preparation for the cerebral diseases comprises gelatin derivative chitosan microspheres coated with stem cell exosomes, and the microspheres are prepared through the method that the stem cell exosomes are coated with gelatin derivatives of a specific structure and then cross-linked with chitosan. The gelatin derivative used in the invention has good surface activity, and can be well compatible with cells and active factors to form a stable inner core coating structure; meanwhile, the surface of the chitosan of which the outer layer is crosslinked with the gelatin derivative is positively charged and can be combined with anions in nasal mucus, so that the retention time of the microspheres in the nasal cavity can be effectively prolonged. Therefore, the nasal delivery preparation not only can slowly release the stem cell exosome, but also improves the absorption efficiency of the stem cell exosome by the nasal cavity, so that the stem cell exosome can reach the brain more effectively.

Described herein are engineered cytokine receptor switches that can include a signal peptide, an extracellular activator binding domain, a hinge, a transmembrane domain, and / or an intracellular signaling domain. Binding of an activator to the activator binding domain can activate cytokine signaling through the intracellular signaling domain. These cytokine receptor switches can be expressed in immune cells, sometimes in combination with a chimeric antigen receptor (CAR), to increase immune cell persistence by promoting adoption of memory-like phenotypes. Also described herein are methods of using engineered cytokine receptors in immune cell therapies, such as CAR T-cell therapy, to improve patient outcomes and prevent disease relapse.

The invention provides a medical-grade recombinant three-type collagen and application thereof. The recombinant humanized three-type collagen has certain cell proliferation promoting capacity and is free of cytotoxicity. The medical-grade recombinant three-type collagen provided by the invention not only has the capability of promoting cell proliferation, is free of cytotoxicity and high in cell adhesion activity, but also has a three-helix structure characterized by the collagen and is extremely high in stability, the purity can reach about 85% after the collagen is induced for 120H in a 100L fermentation tank, the difficulty of a downstream purification process is greatly reduced, and the production cost is reduced. The effect in the aspect of cell activity is obvious.

The invention discloses copper selenide / tricalcium silicate bone cement. The bone cement comprises a liquid phase component and a solid phase component, the liquid phase component is water, and the solid phase component is tricalcium silicate powder and copper selenide nanoparticles. Compared with the existing tricalcium silicate bone cement, the tricalcium silicate bone cement has the advantages that by introducing a trace amount of copper selenide nanoparticles, not only can the hydration and coagulation time of tricalcium silicate be shortened, but also the porosity and degradation rate of the tricalcium silicate after coagulation and solidification can be reduced, the compressive strength can be improved, the stimulation effect on ALP activity of MC3T3-E1 cells can be enhanced, the survival rate of the MC3T3-E1 cells can be improved, and the application prospect is broad. The immunogenic apoptosis of K7M2-wt cells is promoted, and the tumor growth rate is reduced. Besides, the introduction of the copper selenide nanoparticles can also endow the tricalcium silicate bone cement with the performance of temperature rise under near-infrared light, so that in clinical application, the tricalcium silicate bone cement can be further combined with a near-infrared technology to realize ablation of bone tumors, and a new strategy is provided for treatment of the bone tumors.

The invention discloses a bioreactor multi-parameter adaptive control system and method based on machine learning, and belongs to the technical field of bioreactor intelligent control, and the bioreactor multi-parameter adaptive control system comprises a bioreactor hepatocyte culture unit, a data acquisition and control unit and a machine learning intelligent decision-making unit. And the data acquisition and control lower computer controls operation variables such as gas supply, sampling and perfusion in the culture unit execution mechanism, collects culture process data returned by the parameter detection module, constructs a data-driven cell state prediction model by utilizing machine learning, and optimizes model parameters and operation variables based on a prediction result to realize closed-loop control. The method can overcome the defect that there is no precise mechanism model for hepatic cell culture, and multi-parameter collaborative optimization can be realized only depending on process data, so that the state of the hepatic cells is sensed in real time, full-cycle optimization of cell viability and functions is realized, and the activity and density of hepatic cell culture are effectively improved.

Provided is a polypeptide which contains a polypeptide having at least 80%, 85%, 90%, 95%, or 99% identity to amino acid sequence MTYRPGYNPFG (SEQ ID NO: 41) or amino acid sequence AKGYYRPYGVPV (SEQ ID NO: 22), or a polypeptide having one or more amino acid substitutions, deletions and / or additions relative to the amino acid sequence as shown in SEQ ID NO: 41 or 22. The provided polypeptide can interfere with the binding of a Brag2 synaptic protein to the C terminus of an AMPA receptor, thereby inhibiting NMDA-mediated excessive endocytosis of an AMPA receptor and neuronal apoptosis, and playing a role in protecting neuronal cell activity. Therefore, the polypeptide has application prospects and potential development values as a drug for treating nervous system diseases such as strokes, depression, Alzheimer's disease and drug addiction.

The invention relates to the technical field of cell culture, in particular to an oriented porous bionic gelatin scaffold for controlling cell orientation arrangement, a preparation method and application thereof, and the oriented porous bionic gelatin scaffold can be used for cell culture meat production. The preparation method comprises the following steps: mixing and solidifying gelatin and glutamine transaminase, directionally freezing, freeze-drying, sterilizing, inoculating and culturing cells and the like. Parameters and culture medium components in each step are also explained. Compared with the prior art, a regular directional porous structure is formed through a hydrogel scaffold and directional freezing, directional arrangement and growth of fish muscle satellite cells are guided, the cell activity is high, and the proliferation and differentiation effects are good; the gelatin material is adopted, so that the cost is low, the biocompatibility is good, and the gelatin material is superior to collagen and wheat glutelin; the preparation process is simple, does not need complex equipment, is suitable for large-scale production, and promotes the development of cell culture meat.

The invention discloses a medicine screening method based on colorectal cancer organoid, which comprises the following steps: adding a colorectal cancer culture medium into the cultured colorectal cancer organoid, culturing for 48-72 hours, adding a to-be-detected colorectal medicine, continuously culturing for 72-96 hours, detecting the cell activity, and screening the medicine capable of reducing the cell activity. According to the method, the tumor microenvironment of the organoid is utilized, the modeling period is shortened, the cost is reduced, the tumor state can be simulated, high-throughput drug screening can be carried out in vitro, and the method has important significance on accurate treatment of lung cancer.

The invention provides a culture medium and a method for promoting mesenchymal stem cells to secrete exosomes, and belongs to the technical field of cell biology. The culture medium provided by the invention comprises a basic culture medium and the trichostatin A with the final concentration of 10-100nM. The TSA in the culture medium can significantly enhance MSCs exosome secretion under low-concentration and short-time stimulation, does not damage cell activity, does not affect basic biological characteristics and functions of the exosome, significantly increases the MSCs exosome secretion amount by more than 2 times, greatly improves the production efficiency, and provides a new strategy for large-scale production of the exosome.

The invention provides a dynamic regulation and control method for optimizing CAR-T cell amplification conditions, which comprises the following steps: acquiring high-frequency cell activity monitoring data from a cell culture system, and extracting metabolic marker fluctuation and proliferation rate mutation from the high-frequency cell activity monitoring data to obtain real-time cell state parameters; comparing the real-time cell state parameters with a T cell subset proportion and a cell factor release mode in historical immune data of the patient to obtain a cell state deviation value, and determining an abnormal fluctuation condition of proliferation rate mutation according to the cell state deviation value; if the abnormal fluctuation of the proliferation rate mutation exceeds a preset fluctuation threshold value, capturing proliferation signal abnormity by analyzing metabolic marker fluctuation and metabolic waste accumulation according to the cell state deviation value; according to the targeted culture parameter set, adjusting the oxygen supply quantity and the culture solution microenvironment of an oxygen concentration control system to obtain a culture environment for inhibiting cytokine storm and proliferation signal abnormity.

The present disclosure relates to an inhibitor for promoting collagen expression, the inhibitor having a structural formula as shown in the following formula (I). The azacycloalkane compound and a pharmaceutical composition thereof provided in the present disclosure have significant Rho kinase inhibitory activity, have better enzymatic activity and cell activity than existing ROCK inhibitors such as Ripasudil, Netarsudil and belumosudil, and have JAK inhibitory activity. Therefore, the compound and composition have great application potential.

The utility model discloses a cell transfer bag with a novel structure, which comprises a transfer bag which is respectively connected with an input port, a liquid inlet conduit and an output port, and is characterized by further comprising a sampling bag which is communicated with the transfer bag through a plastic hose and is a closed bag body. The cell preparation sampling device is novel in structure and high in practicability, the upper end of the transfer bag is connected with the sampling bag through the plastic hose, when a cell preparation needs to be sampled, 1-2 ml of cell suspension is manually squeezed into the sampling bag, then the plastic hose between the sampling bag and the transfer bag is subjected to heat sealing, after heat sealing is completed, the sampling bag is taken down, and then cell activity and cell quantity detection can be conducted; the sampling is convenient and sterile.

The present invention relates to lipid-based nanoparticles comprising an antigen-binding domain capable of specifically binding to a target expressed on the surface of activated immune cells, and one or more mRNA molecules encoding an activity-enhancing protein of the activated immune cells, and to the use thereof.

The invention discloses an application of samidocardone in preparation of a medicine for treating pulmonary fibrosis. According to the application disclosed by the invention, the condition that the samidojun can inhibit the activity of the pulmonary fibrosis cells and reduce proliferation by inducing the ferroptosis of the pulmonary fibrosis cells is proposed and verified for the first time, and meanwhile, the expression of fibrosis-related proteins is reduced, so that the progress of the pulmonary fibrosis is effectively relieved, and a new direction is provided for treating the pulmonary fibrosis.

The preparation method of the camellia oil fermentation product comprises the following steps that a fermentation base solution containing camellia oil and a strain seed solution are mixed, the strain seed solution comprises at least one of lactobacillus plantarum and saccharomyces cerevisiae, fermentation culture is conducted, and the camellia oil fermentation product is obtained. The fermentation liquor containing the camellia oil leavening is obtained. Compared with a camellia oil fermentation product obtained by fermenting other strains, the camellia oil fermentation product provided by the scheme has higher antioxidant activity (DPPH and ABTS free radical scavenging ability), has stronger ability of inhibiting cells from spontaneously generating NO (nitric oxide), shows good biocompatibility in a cell experiment, and can be used for preparing a camellia oil fermentation product. The obvious adverse effect on the cell activity is not generated.

The present invention belongs to the field of medicines, and specifically relates to a triazole compound, a preparation method therefor and the use thereof, and a compound as shown in formula (I), or a pharmaceutically acceptable salt. The triazole compound of the present invention has a novel structure. The cell activity test proves that the compound of the present invention has a good potential application value in anti-tumor drugs, and can provide a brand-new drug selection for developing anti-tumor and anti-inflammatory drugs.

The application discloses a kind of composition with immunoregulation and anti-aging function and preparation method thereof, and core component is marine collagen peptide, fusion polypeptide CPF-1, targeting senescent cell monoclonal antibody mAb Senolix, five gallate acyl glucose (PGG) and Hericium erinaceus polysaccharide.Fusion polypeptide CPF-1 is designed by domain fusion strategy, with collagen binding, immune activation and antibacterial function;Monoclonal antibody mAb Senolix targets the surface antigen p16^(INK4a) of senescent cell, and removes senescent cell by ADCC effect.The composition significantly improves immune cell activity, promotes collagen synthesis and reduces chronic inflammation through the synergistic effect of multiple components, and shows excellent immunoregulation and anti-aging effect in in vitro cell model and immunosuppressed mouse model, and can be used for developing immune enhancer, anti-aging drug or functional food.