1279 results about "Cell activity" patented technology

The invention discloses an immune cell state analysis method and system, and relates to the technical field of image processing. The method comprises the following steps: acquiring a motion image sequence of immune cells by using microscopic imaging equipment; performing edge detection on the motion image sequence, and marking contour pixel data labels of immune cells; performing trajectory tracking on the immune cells under a time sequence, and outputting motion trajectory pixel data; inputting the motion trail pixel data into a dynamic change rate recognition model, obtaining a first-order change rate, a second-order change rate and a change rate mean value, and generating a dynamic change feature embedding vector; inputting into an immune cell state prediction large model for state prediction to obtain an active state prediction index, and performing active labeling on the immune cells according to the active state prediction index. The technical problems of inaccurate analysis and incomplete prediction of the immune cell activity state in the prior art are solved, and the technical effect of improving the accuracy and comprehensiveness of immune cell activity state prediction is achieved.

The invention provides a culture medium for immune cells as well as a preparation method and application thereof. On the basis of a serum-free culture medium, a specific additive is added into the culture medium and is used as a nutrient substance for growth of immune cells, so that the concentrations of various components in the final culture medium are fixed, the culture efficiency of the immune cells is improved, the cell activity is increased, and the repeatability of subsequent experiments is ensured. Meanwhile, due to the use of a specific additive, animal serum protein added into a conventional culture medium is avoided, so that the risk of infection of exogenous viruses and pathogenic factors is avoided, the immune cells obtained by culture have no sensitization, and the condition that a receptor is allergic to exogenous serum is avoided. In addition, the cost of the immune cell culture medium is reduced, and the preparation process of the culture medium is simplified. Compared with the prior art, the additive provided by the invention can be preserved for a long time in a low-temperature environment, the activity is kept unchanged, and the additive can be taken and used when a culture medium is prepared, so that the preservation cost of immune cells is effectively reduced.

The invention discloses tissue cell activity detection equipment, and relates to the technical field of cell activity detection.The tissue cell activity detection equipment comprises a detection box, a box door is hinged to the outer wall of one side of the detection box, and a culture plate is slidably connected to the bottom of an inner cavity of the detection box; at least four sliding grooves are symmetrically formed in the inner wall of the detection box, a detection person pulls an operation handle to promote a connecting base to slide along the surface of a limiting column, a gear is meshed with a plurality of racks to promote the gear to drive a liquid storage tank to rotate by 180 degrees in a reciprocating mode, and then cell suspension is continuously shunted through a plurality of stop levers. Therefore, the liquid storage tank can be continuously rotated when the cell suspension is dripped, so that the cell suspension can be prevented from being in a static state for a long time to generate a precipitation phenomenon, the cell density distribution of the cell suspension in the holes in the culture plate can be ensured to be uniform, the accuracy and the reliability of a detection result can be improved, and the detection efficiency is improved. The quality of detection data can be improved, and meanwhile, the time and the cost of repeated detection due to inaccurate detection results are reduced.

Disclosed are a standing wave acoustofluidic device for sorting exosomes in body fluids and a method for using same. The standing wave acoustofluidic device for sorting exosomes in body fluids comprises an upper microfluidic chip and a lower acoustic wave excitation apparatus. The microfluidic chip comprises a channel chip and a cover plate, and the acoustic wave excitation apparatus comprises a piezoelectric transducer and an acoustic wave coupling liquid layer. The piezoelectric transducer is reversibly connected to the cover plate by means of the acoustic wave coupling liquid layer. The piezoelectric transducer is a piezoelectric ceramic. The channel chip is provided with a body fluid sorting channel, and the cover plate is provided with a micro-channel groove array structure corresponding to the body fluid sorting channel. The micro-channel groove array structure adjusts acoustic waves excited by the piezoelectric transducer, such that the acoustic waves are modulated in a space composed of the channel chip and the cover plate to form a required standing wave acoustic field. The present invention has advantages such as not affecting cell activity, being unaffected by temperature and the like, offering a stable sorting process, a simple manufacturing process, and low regulation difficulty, and eliminating the need to match the width, depth, and the like of microfluidic channels with the half-wavelength of acoustic waves.

The invention discloses pediococcus pentosaceus CCFM1402 capable of effectively repairing a skin barrier and a self-fermentation metabolite of the pediococcus pentosaceus CCFM1402, and belongs to the technical field of microorganisms and medicines. The pediococcus pentosaceus CCFM1402 provided by the invention has the effect of improving the skin barrier function in both external use and oral administration; the cell activity of damaged HaCaT cells and the expression of the gene level of skin barrier function protein and tight junction protein can be improved by virtue of a thallus lysate and a fermented supernatant (metagen) of the external pediococcus pentosaceus CCFM1402; and the DNFB-induced mouse skin barrier injury can be relieved in the aspects of appearance, skin barrier protein expression and the like by orally taking viable bacteria and postbiotics of the pediococcus pentosaceus CCFM1402. Therefore, the pediococcus pentosaceus CCFM1402 has a huge application prospect in preparation of external or oral medicines for repairing the skin barrier and preparation of food, health care products or cosmetics.

A dual screen technique using DEL and 2DCS screens with libraries of synthesized small molecules and synthesized RNA structures (4,096 targets) delivers bona fide ligand-RNA 3D fold target pairs. One of the newly discovered ligands bound a 5′GAG / 3′CCC internal loop that is present in pri-miR-27a, the oncogenic precursor of microRNA-27a. The DEL-derived pri-miR-27a ligand is cell-active, potently and selectively inhibits pri-miR-27a processing to reprogram gene expression and halt an otherwise invasive phenotype in triple-negative breast cancer cells.

The invention discloses a method for identifying the stability of granular sludge based on microbial activity change characteristics. The method comprises the following steps: taking a certain amount of sludge at the aeration tail end of an AGS device as a sample; the method comprises the following steps: putting a sample into a transparent measuring cylinder, judging the settling performance of granular sludge, quantitatively detecting the cell activity of microorganisms in the sludge by using a flow cytometer, and meanwhile, carrying out online monitoring on the sludge sample of an aerobic granular sludge system by using a respiration graph measuring device (LB-WBM-450PI type smart workstation). Through multi-dimensional analysis of sedimentation characteristics, microbial metabolic activity and flow cytometry activity of the aerobic granular sludge, characteristic information such as particle morphological structure integrity, dynamic change between the microbial metabolic activity and the flow cytometry activity and the like can be provided, and data support is provided for stability regulation and control research of the aerobic granular sludge.

The invention relates to an organ-like core-shell microsphere as well as a preparation method and application thereof. The preparation method comprises the steps that 1, raw materials are prepared, specifically, shell raw materials and inner core raw materials are prepared, the shell raw materials comprise a photoinitiator and methacrylated hyaluronic acid (HAMA), and the inner core raw materials comprise organoid precursor cell suspension and matrigel; 2) molding: extruding the dispersion phase of the shell raw material wrapping the core raw material to a continuous phase by using a micro-fluidic chip and a high-precision injection pump, cutting the dispersion phase into liquid drops by the continuous phase, and performing illumination curing on the liquid drops to form core-shell microspheres; and (3) incubating, namely culturing the core-shell microspheres in a culture medium, so that the organoid precursor cells in the inner core are developed into organoids. According to the preparation method, rapid forming and curing of the organ-like precursor cells carried by the matrigel are achieved, so that the time of the organ-like precursor cells staying in the oil phase is shortened, the cells can obtain oxygen and nutrient substances, and the cell activity is improved.

The invention discloses preparation of a whey protein peptide mixture with immune and muscle-building effects, and relates to the technical field of food processing and bioengineering. The neutral protease is adopted and the pH is adjusted, so that the enzymolysis efficiency is effectively improved, and the enzymolysis time is greatly shortened; meanwhile, the whey protein peptide which is uniform in molecular weight distribution, free of peculiar smell and good in thermal stability is successfully prepared by accurately controlling enzymolysis conditions and combining microbial fermentation. The protein peptide can effectively improve thymus indexes and spleen indexes, enhance the proliferation capacity of splenic lymphocytes and the activity of NK cells, play an excellent immune regulation role and have a remarkable muscle building effect. The preparation process has the advantages of high efficiency, low cost, simplicity, convenience and the like, gives consideration to high quality and high functionality of the product, and has great application value.

The invention discloses application of an MDH2 inhibitor in preparation of a preparation for reducing toxicity of fumonisins, and belongs to the technical field of biology. A fumonisins treatment experiment is carried out on MDH2 gene mutant cells and wild type MDH2 gene cells, and the result shows that the cell activity of the MDH2 gene mutant cells is remarkably improved by 15.2% compared with that of wild type MDH2 gene expression cells under the condition that the fumonisins concentration is 100 micrograms / mL, the cell apoptosis rate is remarkably reduced by 7.76%, and the cell apoptosis rate can be reduced by 7.76%. Therefore, the MDH2 gene mutation can improve the tolerance to the fumonisins. In addition, the LW6 inhibitor is used for inhibiting the MDH2 activity of the wild type IPEC-J2 cell, and the tolerance of the cell to the fumonisins can also be improved. The invention provides a potential solution way for preventing adverse reaction caused by fumonisins, and provides a theoretical basis for preventing and controlling harm of FB1 to food and animal husbandry.

The invention provides a method for distinguishing cell activity behaviors in a culture medium under the assistance of a computer, and relates to the technical field of cell image processing, and the method comprises the following steps: obtaining a target microscopic image of a target cell at the current moment, extracting the static characteristics of the target cell in the target microscopic image, and if the recognition confidence is lower than a first threshold value, determining that the target cell is a cell activity behavior; if yes, calling a historical image sequence of an area where the target cell is located; at least N candidate images are obtained through screening in the historical image sequence, time sequence dynamic characteristics of the target cells are obtained based on the candidate images, and the time sequence dynamic characteristics comprise the motion trail, the motion speed, the morphological change related to the activity behavior and the periodic change condition presented by the target cells within a certain time period; and combining the time sequence dynamic characteristics with the static characteristics, and carrying out activity behavior matching to obtain activity behaviors of the target cells. According to the method, the robustness and the accuracy of cell behavior recognition in a complex culture environment are remarkably improved through static-dynamic feature fusion.

The invention relates to the field of tea fermentation, in particular to a preparation method and application of black tea fermentation liquor with an anti-aging effect. The black tea fermentation liquor rich in tea polyphenol, thearubigins, theaflavins and theabrownin is prepared by jointly fermenting black tea with pichia kudriavzevii and bacilli. The black tea fermentation liquor has the functions of regulating cell metabolism and functions, repairing oxidative damage of cells, keeping completeness of mitochondrial functions, promoting synthesis of a high-energy phosphate compound ATP, enhancing cell activity and resisting aging, and can be applied to food, cosmetics and health care products. The fermentation process provided by the invention is green, safe and simple, and has practical application significance.

The invention provides a single-cell suspension and a preparation method thereof. Specifically, the preparation method comprises the following steps: 1) mixing a condensed-state cell sample to be dispersed with a pretreatment solution, and carrying out ultrasonic treatment; 2) mixing the sample obtained in the step 1) with the enzymatic hydrolysate, and carrying out an enzymatic hydrolysis reaction; 3) adding a nanometer material with the surface modified with a target cell specific marker, controlling the distribution of the nanometer material through an external magnetic field, and enriching target single cells; 4) separating the sample obtained in the step 3) to obtain a target single-cell suspension; wherein the conditions of the ultrasonic treatment and the enzymolysis reaction are controlled by an intelligent control system. According to the technical scheme, ultrasonic treatment and enzymolysis reaction are combined, an intelligent control system is used for controlling ultrasonic conditions and enzymolysis conditions respectively, and a nano material labeling technology is combined, so that the finally obtained cell suspension is high in cell viability and high in purity.

The invention provides application of a compound for regulating and controlling an METTL3 target spot in preparation of an anti-enterovirus drug, and belongs to the field of biological medicine, the compound is AN-465 / 42162872, and the compound has a relatively strong anti-EV71 effect in both in-vitro and in-vivo infection models. An in-vitro cell experiment, EV71 infected C57BL / 6 suckling mouse model, m6A methylation sequencing and other methods are utilized to research the activity and potential action mechanism of anti-EV71, and the result shows that AN-465 / 42162872 can inhibit duplication of EV71 in a human RD cell strain in a dose-dependent manner under the condition of maintaining relatively good cell activity, so that the application of AN-465 / 42162872 in the preparation of the EV71 drug is developed. The action mechanism is mainly as follows: the methylation level of the 5002 adenine in the 2C region of EV71 nucleic acid is remarkably reduced by regulating the METTL3, so that virus replication is inhibited, and the METTL3 has relatively high anti-EV71 drug utilization value.

The invention relates to the technical field of biological medicines, and particularly discloses application of gambogic acid in preparation of a medicine for treating ulcerative colitis. The gambogic acid provided by the invention can be used for preparing a programmed necrosis inhibitor and a medicine for treating ulcerative colitis, and the gambogic acid is beneficial to improving cell morphology, increasing cell viability and improving symptoms of ulcerative colitis caused by DSS. Chemical structure of gambogic acid: # imgabs0 #

The invention provides cubilose peptide derived from cubilose and capable of promoting silk polyprotein and inhibiting expression of inflammatory factors and application of the cubilose peptide. The amino acid sequence of the peptide is as shown in at least one of SEQ ID NO: 1-5. The peptide disclosed by the invention is derived from cubilose, has the performance advantages of being small in molecular weight, promoting the expression of cell filament protein, inhibiting the expression of cell inflammatory factors and reducing the level of reactive oxygen free radicals of cells, has the biological activity of moisturizing, resisting inflammation and resisting oxidation, can be used for preparing medicines, daily chemical products, moisturizing products, anti-oxidation products or anti-inflammatory products, and is wide in application prospect.

The invention provides a uterine muscle cell tension analysis method based on a uterine muscle cell activity image, and relates to the technical field of image processing. The method comprises the steps of tension analysis mode obtaining, resting state analysis mode tension analysis, uterine contraction state analysis mode tension analysis and tension analysis result feedback. According to the method, a tension analysis mode is obtained, if the tension analysis mode is a resting state analysis mode, a resting uterine muscle cell image is obtained, and a resting uterine muscle cell tension analysis result is obtained, and if the tension analysis mode is a uterine contraction state analysis mode, a uterine contraction uterine muscle cell image is obtained, and a uterine contraction uterine muscle cell tension analysis result is obtained. Finally, the uterine muscle cell tension analysis result is transmitted to a preset medical staff terminal, the reliability of the uterine muscle cell tension analysis result is improved, and the problem that in the prior art, the reliability of the uterine muscle cell tension analysis result is unstable due to the difference of ultrasonic probe scanning is solved.

The invention discloses high-purity recombinant collagen as well as a preparation method and application thereof, the recombinant collagen is composed of a recombinant collagen monomer, and the recombinant collagen monomer has an amino acid sequence as shown in any one of SEQ ID NO: 1-22. According to the present invention, the easy-to-break site and the hydrophobicity of the collagen are optimized, such that the obtained recombinant collagen monomer and the prepared recombinant collagen have characteristics of high cell activity (adhesion, migration and proliferation), high triple helix structure stability, high purity, no cytotoxicity, large-scale production, and production cost reduction.

The present invention relates to the fields of medicine and cosmetology, and discloses a cell active peptide composition that promotes the regeneration of multi-type collagen structural proteins, its preparation method, and its application in medicine and cosmetology. The composition uses raw materials such as tripeptide-10 citrulline, hexapeptide-9, hydrolyzed sodium hyaluronate, γ-aminobutyric acid, decarboxylated carnosine hydrochloride, acetyl tetrapeptide-3, acetyl tetrapeptide-11, palmitoyl dipeptide-7, and palmitoyl pentapeptide-4. The composition can promote the coordinated expression of skin type I, type III, type IV, type VII, type XVII collagen, fibronectin, elastin, and glycosaminoglycans; prevent collagen cross-linking, maintain triple helix structure, and inhibit collagen degradation. Applied to dermatology and cosmetology, it can accelerate the regeneration of structural proteins such as skin collagen, repair skin damage, reduce wrinkles such as crow's feet, tear groove lines, nasolabial folds, and under-eye lines, solve the skin aging problem caused by the loss of structural proteins such as collagen, and improve skin elasticity and firmness.

The invention belongs to the technical field of cell biology, and particularly relates to a method for detecting cell stability, a cell sample is detected in three aspects of cell performance, cell mutation and cell stability, and when the cell proliferation effect is good, the cell does not migrate, and the cell activity is gt; the cell of which the cell concentration is 80%, the cell early-stage and late-stage apoptosis ratio is less than or equal to 10%, the cell autophagy ratio is less than 5%, no proto-oncogene is remarkably expressed, the cell aging ratio is less than or equal to 20% and the cell multiplication time is less than 48 hours is a stable cell; if one of the indexes does not reach the standard, it is determined that cell passage is unstable, and the cell culture process is stopped. The method provided by the invention can comprehensively judge the life cycle of the cell, so that the cell is applied to the optimal economic / efficient service life, the cell state can be effectively and accurately judged, and the method has good reliability.

The present invention relates to a chimeric costimulatory antigen receptor (CoStAR) useful in adoptive cell therapy (ACT), and cells comprising the CoStAR. The CoStAR can act as a modulator of cellular activity enhancing responses to defined antigens. The present invention also provides CoStAR proteins, nucleic acids encoding the CoStAR and therapeutic uses thereof.

The invention relates to the field of image processors and biological medicines, and discloses an NK cell activity rapid detection method based on image processing. The method comprises the following steps: acquiring an unmarked time sequence phase image sequence of an NK cell and target cell co-culture system; performing cell instance segmentation to track individual cells; extracting a morphological dynamic characteristic parameter set of the target cell, wherein the morphological dynamic characteristic parameter set comprises a volume change rate, a phase gradient entropy, a cytoplasm phase fluctuation frequency and a nuclear region phase mean value; inputting the parameters into a pre-trained death state discrimination model, and outputting a death probability; and calculating a killing efficiency index based on the death probability evolution curve, and judging the activity level of the NK cells. The system comprises a phase image acquisition unit, a cell segmentation unit, a feature extraction unit, a death judgment unit and an activity judgment unit. Through unmarked imaging and deep learning fusion analysis, high-precision, real-time, quantitative and ultra-early NK cell activity evaluation is realized, and the method is suitable for clinical instant inspection and immunotherapy monitoring.

The present application is directed to methods and compositions that are useful for reducing the number of myeloid-derived suppressor cells (MDSC), tumor associated macrophages (TAM), or both in a subject. The methods include administering an antigen binding protein that binds to an antigen expressed by MDSC and / or TAM, or administering a modified T cell or NK-92 cell that expresses an antigen binding protein that binds to an antigen expressed by MDSC and / or TAM, or a combination of both, to a subject. For example, the antigen binding protein can bind to CD33 expressed by MDSC and / or TAM. The methods and compositions are useful for treating a disease associated with MDSC and / or TAM infiltration into a tissue or tumor.

The invention relates to the technical field of biomedical engineering and microfluidics, in particular to a fluid dynamics topological optimization design method of a circulating tumor cell sorting structure, which comprises the following steps of: establishing a three-dimensional flow field model containing a key separation area in a preset design domain, dispersing by adopting a tetrahedral mesh and setting boundary conditions; the method comprises the following steps: establishing a multi-objective optimization function based on a maximized transverse focusing distance, a minimum shear force peak value and a total pressure drop, dispersing a design domain into units and endowing the units with density variables, and realizing global automatic topological optimization by adopting penalty material interpolation and minimum flow velocity threshold constraint. An optimization result is subjected to structural reconstruction processing to generate a CAD model adaptive to micro-nano machining, closed-loop feedback is formed through secondary verification and experimental verification of computational fluid mechanics, it is ensured that the design meets the requirements for sorting efficiency and cell activity, limitation of a traditional flow channel form is broken through, and the design freedom degree, manufacturability and application value are improved.

The invention relates to a preparation method of a 3D printing high-density cell tubular vascular graft. The preparation method comprises the following steps: sucking a supernatant obtained by centrifuging a cell suspension away and only remaining bottom pure cells; the preparation method comprises the following steps: uniformly stirring and mixing OMA, pig gelatin and an LAP photoinitiator in a Hank's solution to form a hydrogel precursor solution; extruding the pure cells and the hydrogel precursor solution from a three-axis nozzle by using printing equipment, and immersing the pure cells and the hydrogel precursor solution into a calcium chloride solution; lyase is added to degrade the hydrogel precursor solution, the hydrogel precursor solution is completely degraded, and an independent single-layer pipeline structure with only a remaining pure cell layer is obtained. The invention provides a method for manufacturing high-density pure-cell lumen constructs to mimic native blood vessels by using a self-made microfluidic coaxial 3D bioprinting device. And after the support hydrogel on the inner layer and the outer layer is removed, a single-layer tubular structure of high-density MOVAs only limited to pure cells is obtained. The cells in the pure cell vascular constructs exhibit good cell viability and cell function.

Disclosed are devices, systems, and methods for ultrasonically stimulating brain tissue using operating parameters associated with a. target region of a brain. The operating parameters can be determined, for example, by combined ultrasonic stimulation and photometry devices and methods. The operating parameters can include pulse repetition frequency, temporal compression, and intensity. Using selected operating parameters, ultrasound stimulation of the target region of the brain can excite or inhibit brain cell activity.

Provided are a recombinant collagen, an expression method and use thereof. In the present disclosure, the recombinant collagen is expressed by a human embryonic kidney cell Expi293F at a high protein expression level, thereby realizing the expression of a large-molecular-weight recombinant collagen. Activity studies have showed that the expressed recombinant collagen has desirable activity in promoting cell migration, while the recombinant collagen III+I with a fusion tag shows high stability at 4° C. In addition, cell activity studies have shown that the recombinant type III collagen expressed by human cells has better activity in promoting the cell migration than that of a commercially available type III collagen. These results indicate that the human cell expression system is conducive to the expression of highly active and large-molecular-weight recombinant collagen.

The invention discloses an effectiveness analysis method of a drug entrapment material for treating intrauterine adhesion. The effectiveness analysis method comprises the following steps: performing multi-aspect research on the drug entrapment material and generating an effectiveness analysis result. In performance characterization, the morphology and pores of the material are analyzed by using microscopy, specific surface area and other technologies, the compatibility of the drug and the carrier is verified, and the physical state of the drug is detected; in-vitro analysis covers drug release kinetics, the release amount is measured in a simulated environment, and a curve is fitted; the biocompatibility evaluation refers to co-culture of a material leaching solution and cells, detection of cell activity and the like; implanting a medicine carrying material into the animal model in vivo, and evaluating the curative effect by using technologies such as image and pathology; and finally, integrating various results to obtain an entrapment material effectiveness analysis conclusion. According to the method, the limitation of traditional single-point testing is broken through, a full-chain evaluation model of structural characteristics-release behavior-biocompatibility-repair efficiency is constructed, and a scientific basis is provided for rational design and clinical transformation of intrauterine adhesion treatment materials.

The invention discloses a closed double-layer stirring type photobioreactor, and relates to the technical field of microalgae culture. A double-layer special-shaped paddle combination is formed through the composite design of a four-straight-blade overall open turbine type (SK) and a three-blade left-handed push type spiral stirring paddle (TXL), paddle combination optimization is formed through the cooperative flow field design of the TXL paddle (lower layer) and the SK paddle (upper layer), axial-radial flow coupling is achieved, and the material exchange rate between gas and liquid is increased; according to the combined design of the double-layer special-shaped paddles, the proportion of a high-shear area is reduced to be lower than 12% through a wide-area low-strength shear mode of the TXL paddles, the shear stress is greatly reduced, and the problem that the shear stress is too high is solved; a dynamic rotating speed strategy is convenient to implement, and the mass transfer efficiency and the cell activity are both considered by adjusting the rotating speed in stages.

The invention relates to the technical field of animal experiments, and discloses a method for constructing a myocardial ischemia animal model, which comprises the following steps: calculating morphological characteristics of contrast mesenchymal stem cells in a preprocessed image, carrying out principal component analysis on the morphological characteristics, and outputting cell activity corresponding to the principal component through an activity analysis model; the method comprises the following steps: constructing an animal three-dimensional model of a myocardial injury animal, and collecting a culture environment of experimental mesenchymal stem cells; the method comprises the following steps: selecting a growth environment in a myocardial injury animal by utilizing a culture environment, collecting an environment image of the growth environment, and selecting a transplantation position of the myocardial injury animal from the environment image; and determining a three-dimensional position of a transplantation position from the animal three-dimensional model, constructing a transplantation path from the surface position to the three-dimensional position of the animal three-dimensional model, and transplanting the experimental mesenchymal stem cells to the transplantation position to obtain the orthotopic transplantation animal model. According to the invention, the cell activity recognition process capable of repairing myocardial injury can be realized, and sufficient growth space of cells is ensured.