51 results about "Binding properties" patented technology

The present invention addresses the problem of providing a humanized anti-CKAP4 antibody, or an antigen-binding fragment thereof, having good binding properties with respect to CKAP4 and also having an anti-tumor action. The problem is solved by a humanized anti-CKAP4 antibody, or an antigen-binding fragment thereof, comprising: a heavy chain variable region that includes a heavy chain CDR1 having the amino acid sequence represented by SEQ ID NO: 1 or 6, a heavy chain CDR2 having the amino acid sequence represented by SEQ ID NO: 2 or 7, and a heavy chain CDR3 having the amino acid sequence represented by SEQ ID NO: 3 or 8, and that includes an amino acid sequence having at least 90% identity to the amino acid sequence represented by SEQ ID NO: 4 or 9; and a light chain variable region that includes a light chain CDR1 having the amino acid sequence represented by SEQ ID NO: 11, a light chain CDR2 having the amino acid sequence represented by SEQ ID NO: 12, and a light chain CDR3 having the amino acid sequence represented by SEQ ID NO: 13, and that includes an amino acid sequence having at least 90% identity to the amino acid sequence represented by SEQ ID NO: 14.

The purpose of the present disclosure is to provide a molecule that shows different binding properties between a full capsid and an empty capsid of AAV. An antibody is provided that has a higher binding ability to an AAV empty capsid than to an AAV full capsid, under a condition (I) of pH 7.4 or a condition (II) of pH 3.0.

This invention discloses a lactide-Nisin solid dispersion and its preparation method. The method includes preparing a mixture of lactide and a hydrophobic solvent; dispersing Nisin in the mixture obtained in the previous step to obtain a multi-component mixture; and cold-treating the multi-component mixture to obtain a solid mixture containing lactide and Nisin. This invention fully utilizes the binding properties of lactide and Nisin, effectively enhancing the stability of Nisin in complex environments, further expanding the application potential of the prepared antibacterial agent, and providing a new approach for developing multifunctional green food preservatives.

Due to the constituent components of the cell walls of filamentous fungi, the texture when eating a filamentous fungus body resembles a paper-like texture, and applications other than meat analogs are limited. Additionally, filamentous fungus bodies have characteristics of absorbing water but also readily releasing water, and having no binding property between mycelia, and thus even when a crushing treatment is performed, it has been difficult to process the result into foodstuffs that have a feeling of unity due only to a filamentous fungus culture product, or into foodstuffs that have uniform and smooth paste-like physical properties. This edible filamentous fungus body having an adjusted texture can be provided by treating a filamentous fungus body with a cell wall degrading enzyme.

The invention discloses a PTFE / modified polyimide composite film with high temperature bonding performance, a preparation method and application thereof, and belongs to the technical field of polyimide films. The method comprises coating FPAA glue on the surface of a PI film to obtain a multilayer modified polyimide film, and then coating PTFE resin on the surface of the multilayer modified polyimide film, drying, and curing to obtain a PTFE / modified polyimide composite film; the FPAA glue is prepared from aromatic diamine and aromatic dianhydride. The FPAA of the invention has good bonding with PTFE and can replace FEP as a functional layer bonded to PTFE. The PTFE and modified polyimide of the prepared composite film have the advantage of not peeling off after multiple heating, and can be applied to special environment fields such as high temperature resistance, chemical corrosion resistance, and special cable preparation.

A binder for secondary battery electrodes, which enables the achievement of a secondary battery electrode that has higher binding properties than ever before contains a block polymer that has a polymer block (A) and a polymer block (B), and it is configured such that: the polymer block (A) includes less than 30% by mass of a structural unit derived from (meth)acrylic acid to a total structural units of the polymer block (A); and the polymer block (B) includes 30 mass % or more and 100 mass % or less of a structural unit derived from (meth)acrylic acid to a total structural units of the polymer block (B).

Provided are methods for efficiently and comprehensively screening antibody repertoires from B cells to obtain and produce molecules with binding characteristics and functional activities for use in human therapy.

The present invention is a method for manufacturing a treated substrate having a surface of which at least a partial region is modified, the method comprising: a step for exposing the surface of a substrate (10) to a surface modifier containing a compound (A) (20) having binding properties with respect to the substrate (10); and a step for cleaning the exposed substrate (10) with a cleaning solution to obtain a treated substrate (100) in which a film is formed by controlling the compound (A) (20) in the plane direction and the height direction of the substrate (10). The method is characterized in that a cleaning solution in which the distance (Ra) between the Hansen solubility parameter of the compound (A) (20) and the Hansen solubility parameter of the cleaning solution satisfies the relationship (Ra) 2 < = 128 is selected.

The invention provides a Trop2-combined nano antibody as well as a product and application thereof, and relates to the technical field of antibodies. The invention discloses a nano antibody specifically binding to Trop2 protein, and a VHH sequence and a corresponding CDR sequence of the nano antibody. The nano antibody has the capability of high-affinity binding to target protein, and the nano antibody subjected to humanized modification still keeps the high-affinity binding characteristic.

Provided is an amino acid recognition agent having improved binding properties, which is capable of obtaining more structural information from a polypeptide based on the kinetics of the on-off binding between the recognition agent and the polypeptide. The amino acid recognition agent may comprise an amino acid binding protein having an engineered binding pocket with one or more modifications relative to a homologous protein. Compared with an unmodified homologous protein binding pocket, the modified binding pocket can increase the number of interactions formed between the binding pocket and an amino acid ligand, and can increase the number of types of amino acid ligands capable of detecting binding compared with the unmodified homologous protein binding pocket. According to the present invention, the amino acid ligands of one or more types can be bound to one or more types of amino acid ligands, and can improve kinetics (e.g., KD, koff, kon) binding to one or more types of amino acid ligands, which advantageously increases the amount or confidence of structural information obtained from polypeptide analysis.

An water-in-oil emulsion composition that enables imparting binding properties and juiciness to a meat substitute processed product comprising plant raw material. The water-in-oil emulsion composition for use in a meat substitute processed product comprising plant raw material includes an emulsifier A, and an emulsifier B and / or an emulsifier C. The emulsifier A is selected from the group consisting of a polyglycerin-condensed ricinoleic acid ester, a glycerin fatty acid ester, a sucrose fatty acid ester, and a lecithin, and has an HLB of 9.0 or less. The emulsifier B is a fatty acid ester and has saturated a fatty acid as a constituent fatty acid. The emulsifier C is a fatty acid ester and has only an unsaturated fatty acid as constituent a fatty acid.

The invention provides an anti-TIGIT antibody and IL-15 fusion protein and application thereof, and specifically, the fusion protein comprises a first binding region having IL-15 receptor binding activity, a second binding region having IL-15 receptor binding activity, and a third binding region having IL-15 receptor binding activity; and a second binding region, the second binding region having a TIGIT binding activity. The fusion protein can be combined with TIGIT and IL-15 receptor protein molecules, an anti-TIGIT antibody relieves an immunosuppressive signal of TIGIT, the fusion protein is promoted to be enriched into TIGIT high-expression cells and tissues by utilizing the targeted combination characteristic of the anti-TIGIT antibody, meanwhile, IL-15 promotes proliferation and maintenance of immune cells and enhances the anti-tumor immune function, and the fusion protein has good clinical application value.

The utility model relates to the technical field of electronic wire harnesses, in particular to an electronic wire harness with a self-binding property, and compared with the prior art, the electronic wire harness with the self-binding property effectively solves the problems that a wire body is protected and isolated through a fixing assembly by placing a wire into a combined groove, and the wire body is not easy to store due to too many wire harnesses of a product. The problems that wires are fixed through fixing blocks, combined grooves and fixing assemblies are used for storing and classifying the wires and protecting the interior of the wire body are solved, and the problem that when an existing electronic wire harness is used, due to the fact that the wire harness has a multi-wire structural form, the wire harness is convenient to store or classify, and the wire harness is not convenient to store or classify is effectively solved. The problems that in an existing wire harness, too many wires are bound together through a binding belt, the internal structure of the wire harness is affected after long-time binding, a binding assembly is oxidized, and the binding effect is affected are solved, and therefore the service life of the wires is prolonged.

Provided is a binder for electrodes of lithium secondary batteries that has excellent binding properties, can reduce electrode expansion due to charge and discharge, and has excellent charge and discharge cycle characteristics. [Solution] The electrode binder of the lithium secondary battery according to the embodiment includes an aqueous dispersion of polyurethane obtained by reacting (A) at least one of an aliphatic polyisocyanate and an alicyclic polyisocyanate, (B) a polyol, (C) a compound having two or more hydroxyl groups and a carboxyl group, and (D) a chain extender. The (B) component includes a hydrogenated polybutadiene polyol. The molar ratio (NCO / OH) of the isocyanate groups of the (A) component to the hydroxyl groups of the (B) component and the (C) component is 1.15 or more and less than 1.35.

The present invention relates to a core-shell particle (or a core-shell particle type binder) having a perfluorinated resin core comprising a perfluorinated polymer and a resin shell layer comprising an acrylic polymer, a composition comprising a core-shell particle (or a core-shell particle type binder), or an electrode made from the composition, wherein the core-shell particles are prepared by emulsion polymerizing an acrylic monomer to the perfluorinated resin core using a polyglycol ether having a C8 or greater alkyl group substituted with at least one alkyl group of chemical formula 1 as an emulsifier, and the acrylic monomer comprises (a) methyl methacrylate (MMA), (b) methacrylic acid (MAA) or acrylic acid (AA) and (c) a C2-C6 alkyl (meth) acrylate.

The invention belongs to the technical field of plant biology, and particularly relates to a nucleic acid aptamer with high binding force specificity with arabidopsis thaliana COP1 protein and a preparation method of the nucleic acid aptamer. In order to solve the problem that the existing screening technology wastes time and labor, the invention provides a nucleic acid aptamer specifically bound with arabidopsis thaliana COP1 protein and a preparation method thereof. The nucleic acid aptamer Lib2-11 of arabidopsis thaliana COP1 is obtained through a One-step screening technology, the nucleic acid aptamer has high binding property and high specificity, and the nucleic acid aptamer Lib2-11c with high binding property is obtained after a sequence is optimized. The nucleic acid aptamers Lib2-11 and Lib2-11c disclosed by the invention have important application prospects in determining the protein level of the arabidopsis thaliana COP1, subcellular localization and a light-operated synthesis gene route.

The present invention relates to core-shell particles (or a core-shell particle-type binder), a composition comprising the core-shell particles (or the core-shell particle-type binder), or an electrode manufactured from the composition, the core-shell particles having a perfluorinated resin core, which comprises a perfluorinated polymer, and a resin shell layer, which comprises an acrylic polymer, wherein the core-shell particles are prepared by emulsion-polymerizing an acrylic monomer to the perfluorinated resin core by using, as an emulsifier, a polyethylene glycol ether having an alkyl group of C8 or greater, which is substituted with at least one alkyl of chemical formula 1, and the acrylic monomer comprises (a) methyl methacrylate (MMA), (b) methacrylic acid (MAA) or acrylic acid (AA), and (c) a C2-C6 alkyl (meth)acrylate.

An apparatus for detecting and communicating measurements related to the occurrence, magnitude, and duration of one or more binding events between a targeting agent and a target is disclosed. The apparatus may comprise a sensing subsystem with at least one targeting agent and electrically conductive linkers to enable detection of a target within a chemical or biological environment or sample. A deciding subsystem facilitates the identification of single binding events and their duration. Signaling circuitry processes input signals and transmits binding event-related data to a user device. The apparatus may be housed with additional components to measure binding characteristics of drug candidates, enabling precise, efficient, and cost-effective testing, screening, and validation of drug candidates. Alternatively, the apparatus may be configured and used to analyze spatial and temporal characteristics at the genomic, protein, and post-translational modification levels in cells, tissues, and organs in both in vitro and in vivo contexts.

Disclosed is a covalently-linked multilayered three-dimensional matrix comprising capture molecules, linkers and spacers (referred to as a Molecular Net) for specific and sensitive analyte capture from a sample. Also disclosed herein is a Molecular Net comprising covalently-linked multilayered three-dimensional matrix comprising more than one type of capture molecule and more than one type of linker and may comprise one or more spacer for specific and sensitive capture of more than one type of analyte from a sample. A Molecular Net may comprise a pseudorandom nature. Use of various capture molecules, linkers and spacers in a Molecular Net may confer unique binding properties to a Molecular Net. Porosity, binding affinity, size exclusion abilities, filtration abilities, concentration abilities and signal amplification abilities of a Molecular Net may be varied and depend on the nature of components used in its fabrication. Uses of a Molecular Net may include analyte capture, analyte enrichment, analyte purification, analyte detection, analyte measurement and analyte delivery. Molecular Nets may be used in liquid phase or on solid phases such as nanomaterials, modified metal surfaces, nanospheres, microspheres, microtiter plates, slides, pipettes, cassettes, cartridges, discs, probes, lateral flow devices, microfluidics devices, microfluidics devices, optical fibers and others.

The invention belongs to the technical field of electromagnetic shielding material preparation, and specifically relates to a method for preparing a PVC / LDH electromagnetic shielding film. The invention prepares an LDH / polymer solution by a coprecipitation method, thereby improving the binding property between the polymer and the LDH and avoiding the problem of LDH peeling off in the polymer matrix. Then, a certain water-soluble solvent is added to the LDH / polymer mixed solution to cause part of the polymer to precipitate and deposit on the surface of the LDH layer. The LDH / polymer settles in the solution, and due to the mutual entanglement between the polymer chains, the LDH / polymer has ductility and film-forming characteristics. Then, a surface coating method is used to coat the surface of a non-woven fabric to form an LDH film, and finally a layer of PVC film is coated on the surface of the LDH film to improve the mechanical properties of the film and solve the problem of poor mechanical properties when the content of inorganic nanoparticles is high. By controlling the thickness of the LDH film, PVC / LDH films with different electromagnetic shielding properties are prepared. The preparation process is simple, environmentally friendly, has few by-products, and can reduce industrialization costs.

The present application relates to chimeric DNA polymerases and methods of making the same. The chimeric DNA polymerases comprise 2-8, e.g., 3, domains or segments derived from different polymerases. The chimeric DNA polymerases of the present application have improved properties, e.g., superior extension properties, superior DNA binding properties, superior proofreading activity, superior fidelity, faster amplification speed, superior tolerance to inhibitors, superior long fragment amplification ability, etc.

According to various aspects, the present invention provides a method of delivering a plurality of sample solutions to a sensor surface (102) for analysis. It may additionally or alternatively provide systems and methods for enabling an improved study of analyte behavior. A sensor (102) may be used, for example, to determine kinetic binding properties of an analyte, such as a protein, to a surface binding ligand. In one aspect, a method includes: a) introducing (902) a first sample solution (502) into a first channel (506), the first channel (506) configured to deliver the sample solution to a sensor surface (102); b) introducing (904) a second sample solution (504) into the first channel (506); c) flowing (906) the first sample solution and the second sample solution through the first channel (506) and over the sensor surface (102); and d) detecting (912) at the sensor surface (102) the presence or absence of binding of the analyte from at least one of the sample solutions (502, 504) wherein no running buffer solution passes over the sensor surface (102) throughout steps a)-d).

Provided is a binder for an electrode of a lithium secondary battery, the binder having excellent binding properties, being capable of reducing electrode swelling caused by charge / discharge, and bringing about excellent charge / discharge cycling characteristics.　A binder for an electrode of a lithium secondary battery according to an embodiment comprises an aqueous dispersion of a polyurethane obtained by reacting (A) an aliphatic polyisocyanate and / or an alicyclic polyisocyanate, (B) one or more polyols, (C) a compound having two or more hydroxyl groups and a carboxy group, and (D) a chain extender. The (B) component includes a hydrogenated polybutadiene polyol. The molar ratio (NCO / OH) of the isocyanate groups of the (A) component to the hydroxyl groups of the (B) component and (C) component is 1.15 or higher but less than 1.35.

Provided are: a peptide having good binding properties with isovaleric acid; and a composition for isovaleric acid measurement and an odor sensor each using the peptide. The peptide comprises an amino acid sequence represented by any one of SEQ ID NOS: 1-13. The composition for isovaleric acid measurement and the odor sensor contain a peptide comprising an amino acid sequence represented by any one of SEQ ID NOS: 1-13.

This prediction method to be executed by a computer includes a prediction step (S13) in which biological properties of a test substance are predicted on the basis of physicochemical properties based on at least the electrical charge or the hydrophobicity of the test substance. The biological properties can be at least one property selected from the group consisting of the immunogenicity, the pharmacokinetics, and the nonspecific binding properties of the test substance. The physicochemical properties can be further based on a dipole moment. The physicochemical properties can also be based on at least electrical charge distribution or hydrophobicity distribution. The physicochemical properties can also be based on at least deviation in electrical charge distribution or deviation in hydrophobicity distribution. The electrical charge distribution can also be based on electrical charge and a dipole moment, and the hydrophobicity distribution can also be based on hydrophobicity and a dipole moment.

A chimera DNA polymerase and a preparation method therefor. The chimera DNA polymerase contains 2-8 (for example 3) domains or segments derived from different polymerases. The chimera DNA polymerase has improved properties such as a better extension characteristic, a better DNA binding characteristic, a better corrective activity, a better fidelity, a higher amplification speed, a better tolerance to an inhibitor, and a higher long fragment amplification capability.

The present invention relates to a method for screening engineered antibody Fc regions having modified Fc receptor binding properties, the method comprising the steps of: (a) providing a plurality of cells displaying engineered antibody Fc regions on their cell surface, preferably wherein each cell comprised in the plurality of cells displays an engineered antibody Fc region comprising at least one mutation; (b) contacting the cells in step (a) with an Fc receptor; (c) separating cells that have been contacted with the Fc receptor in step (b) into two or more cell populations according to their ability to bind to the Fc receptor, preferably wherein the ability of an engineered antibody Fc region to bind to the Fc receptor is determined by comparison with a reference antibody Fc region; and (d) sequencing cells from at least one of the populations obtained in step (c), preferably to identify mutations in the engineered antibody Fc region that (i) confer improved binding to the Fc receptor, (ii) do not affect binding to the Fc receptor, and / or (iii) confer reduced binding to the Fc receptor. Further provided herein are methods for predicting the Fc receptor binding properties of an engineered antibody Fc region, as well as methods for generating an engineered antibody Fc region having desired Fc receptor binding properties

According to various aspects, the present invention provides a method of delivering a plurality of sample solutions to a sensor surface (102) for analysis. It can additionally, or alternatively, provide systems and methods for enabling improved investigation of analyte behaviours. The sensor (102) may, for example, be used to determine kinetic binding properties of analytes (such as proteins) to surface bound ligands. In one aspect, a method comprises: a) introducing (902) a first sample solution (502) into a first channel (506), the first channel (506) configured to deliver sample solution to the sensor surface (102); b) introducing (904) a second sample solution (504) into the first channel (506); c) flowing (906) the first sample solution and the second sample solution through the first channel (506) and over the sensor surface (102); and d) detecting (912) the presence or absence of binding of analyte from at least one of the sample solutions (502, 504) at the sensor surface (102), wherein no running buffer solution is passed over the sensor surface (102) throughout steps a)-d).