The invention relates to a narcissus latent virus detection kit and its detecting method that are special for narcissus latent virus detection. The kit comprises: an upstream primer, a downstream primer, an RT Buffer, an RNA enzyme inhibitor, a reverse transcriptase, dNTPs, a PCR Buffer, Mgcl2, Taq DNA polymerase, a positive control, a negative control and RNase-free ddH2O. In the invention, specific primers are designed according to a coat protein CP gene sequence of the narcissus latent virus, which is detected by a reverse transcription-polymerase chain reaction (RT-PCR) technology. With the obvious advantages of rapidity, accuracy, sensitivity, strong operability and simple kit preparation method, the kit and method of the invention are not only suitable for ports of China to conduct rapid detection on the narcissus latent virus of entry and exit narcissus, and also can be used for narcissus latent virus detection and diagnosis, epidemic surveillance as well as early warning and prediction, thus having broad application prospects.