144 results about "Subtilisin" patented technology

The disclosure relates to isolated oligonucleotides comprising duplex regions targeting Proprotein convertase subtilisin kexin 9 (PCSK9), and delivery systems, kits and compositions comprising same, and methods of using same for inhibiting or downregulating PCSK9.

The invention relates to the technical field of biological extraction, and provides a cattle spleen peptide composition as well as a preparation method and application thereof. The preparation method of the cattle spleen peptide comprises the following steps: S1, taking a cattle spleen, adding normal saline, and homogenizing to obtain first homogenate; s2, adding combined enzyme into the first homogenate, uniformly stirring, and performing first water bath treatment to obtain second homogenate; s3, adding chymotrypsin into the second homogenate, uniformly stirring, and performing second water bath treatment to obtain third homogenate; s4, centrifuging the third homogenate to obtain supernate; s5, performing ultrafiltration and drying on the supernate to obtain the cattle spleen peptide. The combined enzyme comprises subtilisin, bromelain and papain. Through the technical scheme, the problem of low cattle spleen peptide extraction rate in related technologies is solved.

The present invention relates to methods for treating diseases or conditions in which proprotein convertase subtilisin / kexin type 9 (PCSK9) expression or activity causes an impact by administration of PCSK9-specific antibodies or antigen-binding fragments thereof and preferably by additional administration of an inhibitor of 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMG-COA reductase). The present invention further relates to PCSK9-specific antibodies or antigen-binding fragments thereof for use in the treatment of diseases or conditions in which PCSK9 expression or activity causes an impact.The present invention also relates to articles of manufacture comprising packaging material, PCSK9-specific antibodies or antigen-binding fragments thereof, and a label or packaging insert indicating which groups of patients can be treated with said antibodies or fragments, which groups of patients must not be treated with said antibodies or fragments, and which dosage regimen should be used.The present invention further relates to methods of testing the efficacy of PCSK9-specific antibodies or antigen-binding fragments thereof for the treatment of certain diseases or conditions and for the treatment of specific sub-groups of patients.

An human antibody or antigen-binding fragment of a human antibody that specifically binds and inhibits human proprotein convertase subtilisin / kexin type 9 (hPCSK9) characterized by the ability to reduce serum LDL cholesterol by 40-80% over a 24, 60 or 90 day period relative to predose levels, with little or no reduction in serum HDL cholesterol and / or with little or no measurable effect on liver function, as determined by ALT and AST measurements.

An human antibody or antigen-binding fragment of a human antibody that specifically binds and inhibits human proprotein convertase subtilisin / kexin type 9 (hPCSK9) characterized by the ability to reduce serum LDL cholesterol by 40-80% over a 24, 60 or 90 day period relative to predose levels, with little or no reduction in serum HDL cholesterol and / or with little or no measurable effect on liver function, as determined by ALT and AST measurements.

The invention discloses a method for preparing a liquid fertilizer from vitamin B12 waste liquid, and relates to the field of liquid fertilizers. When the liquid fertilizer is prepared, the vitamin B12 waste liquid is filtered and then subjected to alkaline pretreatment, and solid protein and filtrate are obtained through separation; acidifying the filtrate with sulfuric acid, adding potassium hydroxide, and concentrating under reduced pressure to obtain regenerated mixed salt; carrying out flash explosion treatment on the solid protein, and carrying out enzymolysis by using trypsin and subtilisin to obtain a proteolysis solution; urea, formaldehyde and dimethyl hydrogen phosphate are mixed to prepare slow-release phosphorus-nitrogen macromolecules; and uniformly mixing the protein enzymatic hydrolysate, the regenerated mixed salt, fulvic acid, slow-release phosphorus-nitrogen macromolecules and deionized water to obtain the liquid fertilizer. The prepared liquid fertilizer has the advantages of excellent long-term effect, easy absorption and waste liquid utilization rate.

The invention relates to an shRNA (short hairpin Ribonucleic Acid) interference sequence of a targeted silence PCSK9 gene and a construction method and lipid-lowering application of a recombinant adeno-associated virus vector of the shRNA interference sequence. Hyperlipidaemia is a metabolic disease characterized by abnormal rising of cholesterol and triglyceride levels in blood, and the design of lipid-lowering drugs is the focus of attention to improvement of hyperlipidaemia. Proprotein convertase subtilisin / kexin type 9 (PCSK9) can be combined with a low-density lipoprotein receptor (LDL-R) and degrade the LDL-R, so that accumulation of LDL-C in blood is further promoted, and hyperlipidemia is caused. Aiming at the key target PCSK9, a specific shRNA interference sequence is designed, and a recombinant adeno-associated virus vector (rAAV) carrying the sequence is constructed by an enzyme digestion-connection method. In-vitro experiments prove that the vector can remarkably reduce the expression level of PCSK9 protein, so that the cyclic utilization of a low-density lipoprotein receptor (LDL-R) is promoted, and the concentration of low-density lipoprotein cholesterol (LDL-C) in plasma is reduced. The rAAV vector provided by the invention has the characteristics of low production cost, high transfection efficiency, lasting action time and the like, and provides a new thought for gene therapy of hyperlipidemia.

The present disclosure relates to small molecule inhibitors of proprotein convertase subtilisin-like / kexin type 9 (PCSK9) pharmaceutical compositions comprising said compounds and their use in prevention and treatment of diseases and conditions associated with PCSK9.

The disclosure relates to inhibitors of PCSK9 useful in the treatment of lipid metabolism, and other diseases in which PCSK9 plays a role, having the Formula (I):or a pharmaceutically acceptable salt, hydrate, solvate, stereoisomer, or tautomer thereof, wherein R1, X1, X2, X3, X4, X5, X6, X7, X8, X9, X10, X11, X12, and X13 are described herein.

Provided are a small interfering RNA (siRNA) for inhibiting expression of proprotein convertase subtilisin / kexin type 9 (PCSK9) in a cell, a vector and a cell comprising a nucleotide encoding the same, and a method for treating a disease or symptom associated with PCSK9 expression in a subject using the siRNA, the vector or the cell.

Disclosed herein is one or more subtilisin variant, nucleic acid encoding same, and compositions and methods related to the production and use thereof, including one or more subtilisin variant that has improved stability compared to one or more reference subtilisin.

The invention provides an anti-aging and skin-tightening composition and a preparation method thereof, and belongs to the technical field of anti-aging and skincare, and the composition comprises a hovenia acerba infructescence extract, an okra seed extract, tetrapeptide-4, acetyl octapeptide-1, a hill-pit snail extract, paeonol and a carnosine-zinc gluconate chelate. And carrying out aerobic fermentation on the raisin tree infructescence powder through aspergillus oryzae, and purifying the centrifuged supernatant through a composite filler resin column to obtain the raisin tree infructescence extract. The preparation method comprises the following steps: sequentially carrying out enzymolysis on okra seeds by virtue of compound enzyme, ficin and subtilisin, adding absolute ethyl alcohol for extraction, centrifuging supernate, and purifying by virtue of an LH-20 sephadex column, so as to obtain the okra seed extract. The preparation method comprises the following steps: sequentially carrying out enzymolysis on the mountain snail meat through trypsin and lumbrukinase, centrifuging the enzymatic hydrolysate, and carrying out ultrafiltration on the supernate to obtain the mountain snail extract with the molecular weight of less than 3kDa. The components are matched for use, so that the balance of high-efficiency anti-aging and low-irritation can be realized through synergism of three major pathways of inhibiting collagen degradation, promoting collagen synthesis and resisting sugar and relieving.

The invention relates to the technical field of cosmetics, in particular to a composition containing PQQ, application of the composition and a skin care product. The composition of pyrroloquinoline quinone or sodium salt thereof and sodium surfactin provided by the invention has good permeability and stability, and meanwhile, has a remarkable synergistic interaction effect in the aspects of oxidation resistance, whitening, bacteriostasis and the like. Wide application prospects are realized in the field of cosmetics. The preparation method of the composition is simple in steps, easy to operate and suitable for industrial production.

Disclosed herein is one or more subtilisin variant, nucleic acid encoding same, and compositions and methods related to the production and use thereof, including one or more subtilisin variant that has improved stability and / or soil removal compared to one or more reference subtilisin.

The invention relates to the technical field of eggplant manufacturing, in particular to a method for manufacturing primary-color high-anthocyanin eggplants, which comprises the following steps: selecting purple eggplants, cleaning, cutting into long strips, soaking the long-strip-shaped eggplants in a honey solution, and adding citric acid into the honey solution; preparing a color protection solution, adding an enzymolysis blocking agent into the color protection solution, and pouring the eggplants into the color protection solution for later use; preparing a soaking solution, soaking the eggplants in the soaking solution for 10 minutes, taking out the eggplants, performing vacuum packaging on the eggplants to obtain the primary-color high-anthocyanin eggplants, and performing cold storage. According to the method, color protection and synergism of the eggplants are achieved through a dual-targeting mechanism, meanwhile, nutrition preservation of the eggplants is achieved, synergistic repair of cell membranes in the eggplants is achieved through combination of ficin protease and bromelain, and triple-effect removal of fungi, bacteria and biological membranes of the eggplants is achieved through combination of natamycin and subtilin.

The invention belongs to the technical field of biological medicine, and particularly relates to application of a biomarker in preparation of a product for diagnosing sepsis, the biomarker is proprotein convertase subtilisin 6 (PCSK6), and the biomarker has remarkably high sensitivity and specificity in the aspect of sepsis diagnosis.

The utility model relates to a device for removing impure protein in crude polysaccharide of traditional Chinese medicine by utilizing immobilized protease, which comprises a protein removal reactor loaded with the immobilized protease, a dissolved oxygen tester and a constant-temperature magnetic stirrer, and a dissolved oxygen electrode of the dissolved oxygen tester is connected to the protein removal reactor. The papain immobilized on the microcrystalline cellulose is used for carrying out enzymolysis reaction on impure protein in the traditional Chinese medicine crude polysaccharide, and the device has the characteristics of simplicity in operation, mild conditions and environment friendliness. The immobilized papain is limited between the two layers of screens, so that the problem of separation of the immobilized papain in the traditional Chinese medicine crude polysaccharide solution can be avoided, and the method has the characteristics of rapidness and convenience. The immobilized papain in the device can be expanded to immobilize other proteases, such as bromelain, subtilisin, protease K and the like, on cellulose according to protein types in different traditional Chinese medicine polysaccharide solutions.

The invention provides a pharmaceutical composition for improving bone mineral density and a preparation method thereof, and belongs to the technical field of biological medicine, the pharmaceutical composition comprises lactarius vomica extract peptide, winged yam rhizome peel extract, rainbow trout extract, calcium citrate-glycine chelate, vitamin K2 and vitamin D3. The lactarius volemus extract peptide is obtained by sequentially carrying out enzymolysis on lactarius volemus by bromelain and subtilisin and purifying and extracting enzymatic hydrolysate through an LH-60 sephadex column. The winged yam rhizome peel extract is obtained by extracting the peels of winged yam rhizome tubers with an ethanol water solution and purifying and extracting the extracting solution with an AB-8 macroporous adsorption resin column. The rainbow trout extract is a product below 5kDa obtained by sequentially performing enzymolysis on fish heads, fish skins and fish scales of rainbow trout through trypsin and lumbrukinase. All the components are compounded according to a specific proportion, and the core effect of improving the bone mineral density is achieved through two-way regulation and control of bone metabolism, optimization of calcium utilization and improvement of safety.

The present invention relates to methods of treating or preventing cholesterol-related diseases (such as hypercholesterolemia, hyperlipidemia or dyslipidemia) using antibodies against proprotein convertase subtilisin / kexin type 9 (PCSK9). Also described are formulations and methods of producing the formulations.

Provided are a small interfering RNA (siRNA) used to inhibit the expression of proprote in convertase subtilisin / kexin type 9 (PCSK9) in a cell, a vector comprising nucleotides encoding same, and a cell, as well as a method using the siRNA, the vector or the cell for the treatment of diseases or symptoms associated with the expression of PCSK9 in a subject.

The invention discloses application of an alpha-amylase / subtilisin inhibitor LASI gene in improvement of drought resistance and salt resistance of crops. The nucleotide sequence of the Ligusticum wallichii LASI gene is as shown in SEQ ID NO. 1; transgenic arabidopsis thaliana plants are obtained through genetic engineering and transgenic identification methods, the drought resistance and the salt resistance of transgenic and wild type arabidopsis thaliana are analyzed through growth indexes and biochemical indexes, and LASI transgenic arabidopsis thaliana with the drought resistance and the salt resistance is successfully obtained. The invention provides a new gene source for cultivating crops with stronger drought resistance and salt tolerance by utilizing a gene engineering technology.

Disclosed herein are one or more subtilisin variants, nucleic acids encoding the same, and compositions and methods relating to the production and use thereof, including one or more subtilisin variants having improved stability and / or soil removal compared to one or more reference subtilisin.

Disclosed herein is one or more subtilisin variant useful for cleaning applications and in methods of cleaning. One embodiment is directed to one or more subtilisin variant, including one or more Bacillus sp. subtilisin polypeptide variant, and one or more cleaning composition comprising one or more such variant.

This invention provides an antigen-binding protein that binds to proprotein converterase subtilisin kexin type 9 (PCSK9), as well as a method for using and producing the antigen-binding protein. [Solution] The present invention provides an isolated neutralizing antigen-binding protein that interacts with proprotein converterase subtilisin kexin type 9 (PCSK9), and which contains a specific amino acid sequence that binds to the PCSK9 protein and reduces the LDLR-reducing effect of PCSK9 on LDLR.

Disclosed herein is one or more subtilisin variant, nucleic acid encoding same, and compositions and methods related to the production and use thereof, including one or more subtilisin variant that has improved stability and / or soil removal compared to one or more reference subtilisin.

Disclosed herein is a wearable drug delivery device including a container filled at least partially with a drug including at least one of a PCSK9 (Proprotein Convertase Subtilisin / Kexin Type 9) specific antibody, a granulocyte colony-stimulating factor (G-CSF), a sclerostin antibody, or a calcitonin gene-related peptide (CGRP) antibody. The wearable drug delivery device may include a needle and an insertion mechanism configured to insert the needle into a patient. A fluid pathway connector may define a sterile fluid flowpath between the container and the insertion mechanism. Optionally, a cannula initially disposed about the needle may be included. The cannula may be retained in the patient at an injection site created by the needle after the needle is withdrawn from the patient. Methods of assembly and operation are also provided.

The present invention belongs to the field of biomedical technologies, and specifically relates to the use of a biomarker in the preparation of a product for diagnosing sepsis. The biomarker is proprotein convertase subtilisin / kexin type 6 (PCSK6), which has significantly high sensitivity and specificity in diagnosing sepsis.

The disclosure relates to isolated oligonucleotides comprising duplex regions targeting Proprotein convertase subtilisin kexin 9 (PCSK9), and delivery systems, kits and compositions comprising same, and methods of using same for inhibiting or downregulating PCSK9.

Disclosed herein is a detergent composition comprising a subtilisin variant that has improved stability and / or soil removal compared to one or more reference subtilisin.

The invention relates to a natural plant extract compound formula with a skin whitening enhancing effect and a preparation method of the natural plant extract compound formula, and belongs to the technical field of biology, and the natural plant extract compound formula comprises the following components: a white eggplant extract, a noctilucent flower extract, a broccoli extract, nicotinamide, resveratrol, lecithin and the like; wherein the eggplant extract is an extract which is less than 3kDa and is obtained by carrying out enzymolysis on eggplant through hemicellulase and pectinase together and then carrying out step-by-step enzymolysis on the eggplant through papain and ficin in sequence; the noctilucent noctilucent flower extract is an extract which is less than 3kDa and is obtained by sequentially carrying out step-by-step enzymolysis on the noctilucent noctilucent flower by virtue of ficin The broccoli extract is an extract which is less than 3kDa and is obtained by fermenting broccoli through streptococcus thermophilus and then carrying out enzymolysis through subtilisin. All the components are matched for use, so that the composition has outstanding anti-oxidation and whitening effects, takes effect quickly, and is safe and non-irritant.