110 results about "Subtilisin" patented technology

The invention relates to the technical field of biological extraction, and provides a cattle spleen peptide composition as well as a preparation method and application thereof. The preparation method of the cattle spleen peptide comprises the following steps: S1, taking a cattle spleen, adding normal saline, and homogenizing to obtain first homogenate; s2, adding combined enzyme into the first homogenate, uniformly stirring, and performing first water bath treatment to obtain second homogenate; s3, adding chymotrypsin into the second homogenate, uniformly stirring, and performing second water bath treatment to obtain third homogenate; s4, centrifuging the third homogenate to obtain supernate; s5, performing ultrafiltration and drying on the supernate to obtain the cattle spleen peptide. The combined enzyme comprises subtilisin, bromelain and papain. Through the technical scheme, the problem of low cattle spleen peptide extraction rate in related technologies is solved.

An human antibody or antigen-binding fragment of a human antibody that specifically binds and inhibits human proprotein convertase subtilisin / kexin type 9 (hPCSK9) characterized by the ability to reduce serum LDL cholesterol by 40-80% over a 24, 60 or 90 day period relative to predose levels, with little or no reduction in serum HDL cholesterol and / or with little or no measurable effect on liver function, as determined by ALT and AST measurements.

An human antibody or antigen-binding fragment of a human antibody that specifically binds and inhibits human proprotein convertase subtilisin / kexin type 9 (hPCSK9) characterized by the ability to reduce serum LDL cholesterol by 40-80% over a 24, 60 or 90 day period relative to predose levels, with little or no reduction in serum HDL cholesterol and / or with little or no measurable effect on liver function, as determined by ALT and AST measurements.

The invention discloses a method for preparing a liquid fertilizer from vitamin B12 waste liquid, and relates to the field of liquid fertilizers. When the liquid fertilizer is prepared, the vitamin B12 waste liquid is filtered and then subjected to alkaline pretreatment, and solid protein and filtrate are obtained through separation; acidifying the filtrate with sulfuric acid, adding potassium hydroxide, and concentrating under reduced pressure to obtain regenerated mixed salt; carrying out flash explosion treatment on the solid protein, and carrying out enzymolysis by using trypsin and subtilisin to obtain a proteolysis solution; urea, formaldehyde and dimethyl hydrogen phosphate are mixed to prepare slow-release phosphorus-nitrogen macromolecules; and uniformly mixing the protein enzymatic hydrolysate, the regenerated mixed salt, fulvic acid, slow-release phosphorus-nitrogen macromolecules and deionized water to obtain the liquid fertilizer. The prepared liquid fertilizer has the advantages of excellent long-term effect, easy absorption and waste liquid utilization rate.

The invention relates to an shRNA (short hairpin Ribonucleic Acid) interference sequence of a targeted silence PCSK9 gene and a construction method and lipid-lowering application of a recombinant adeno-associated virus vector of the shRNA interference sequence. Hyperlipidaemia is a metabolic disease characterized by abnormal rising of cholesterol and triglyceride levels in blood, and the design of lipid-lowering drugs is the focus of attention to improvement of hyperlipidaemia. Proprotein convertase subtilisin / kexin type 9 (PCSK9) can be combined with a low-density lipoprotein receptor (LDL-R) and degrade the LDL-R, so that accumulation of LDL-C in blood is further promoted, and hyperlipidemia is caused. Aiming at the key target PCSK9, a specific shRNA interference sequence is designed, and a recombinant adeno-associated virus vector (rAAV) carrying the sequence is constructed by an enzyme digestion-connection method. In-vitro experiments prove that the vector can remarkably reduce the expression level of PCSK9 protein, so that the cyclic utilization of a low-density lipoprotein receptor (LDL-R) is promoted, and the concentration of low-density lipoprotein cholesterol (LDL-C) in plasma is reduced. The rAAV vector provided by the invention has the characteristics of low production cost, high transfection efficiency, lasting action time and the like, and provides a new thought for gene therapy of hyperlipidemia.

Disclosed herein is one or more subtilisin variant, nucleic acid encoding same, and compositions and methods related to the production and use thereof, including one or more subtilisin variant that has improved stability compared to one or more reference subtilisin.

The invention provides an anti-aging and skin-tightening composition and a preparation method thereof, and belongs to the technical field of anti-aging and skincare, and the composition comprises a hovenia acerba infructescence extract, an okra seed extract, tetrapeptide-4, acetyl octapeptide-1, a hill-pit snail extract, paeonol and a carnosine-zinc gluconate chelate. And carrying out aerobic fermentation on the raisin tree infructescence powder through aspergillus oryzae, and purifying the centrifuged supernatant through a composite filler resin column to obtain the raisin tree infructescence extract. The preparation method comprises the following steps: sequentially carrying out enzymolysis on okra seeds by virtue of compound enzyme, ficin and subtilisin, adding absolute ethyl alcohol for extraction, centrifuging supernate, and purifying by virtue of an LH-20 sephadex column, so as to obtain the okra seed extract. The preparation method comprises the following steps: sequentially carrying out enzymolysis on the mountain snail meat through trypsin and lumbrukinase, centrifuging the enzymatic hydrolysate, and carrying out ultrafiltration on the supernate to obtain the mountain snail extract with the molecular weight of less than 3kDa. The components are matched for use, so that the balance of high-efficiency anti-aging and low-irritation can be realized through synergism of three major pathways of inhibiting collagen degradation, promoting collagen synthesis and resisting sugar and relieving.

The invention relates to the technical field of cosmetics, in particular to a composition containing PQQ, application of the composition and a skin care product. The composition of pyrroloquinoline quinone or sodium salt thereof and sodium surfactin provided by the invention has good permeability and stability, and meanwhile, has a remarkable synergistic interaction effect in the aspects of oxidation resistance, whitening, bacteriostasis and the like. Wide application prospects are realized in the field of cosmetics. The preparation method of the composition is simple in steps, easy to operate and suitable for industrial production.

The invention relates to the technical field of eggplant manufacturing, in particular to a method for manufacturing primary-color high-anthocyanin eggplants, which comprises the following steps: selecting purple eggplants, cleaning, cutting into long strips, soaking the long-strip-shaped eggplants in a honey solution, and adding citric acid into the honey solution; preparing a color protection solution, adding an enzymolysis blocking agent into the color protection solution, and pouring the eggplants into the color protection solution for later use; preparing a soaking solution, soaking the eggplants in the soaking solution for 10 minutes, taking out the eggplants, performing vacuum packaging on the eggplants to obtain the primary-color high-anthocyanin eggplants, and performing cold storage. According to the method, color protection and synergism of the eggplants are achieved through a dual-targeting mechanism, meanwhile, nutrition preservation of the eggplants is achieved, synergistic repair of cell membranes in the eggplants is achieved through combination of ficin protease and bromelain, and triple-effect removal of fungi, bacteria and biological membranes of the eggplants is achieved through combination of natamycin and subtilin.

The utility model relates to a device for removing impure protein in crude polysaccharide of traditional Chinese medicine by utilizing immobilized protease, which comprises a protein removal reactor loaded with the immobilized protease, a dissolved oxygen tester and a constant-temperature magnetic stirrer, and a dissolved oxygen electrode of the dissolved oxygen tester is connected to the protein removal reactor. The papain immobilized on the microcrystalline cellulose is used for carrying out enzymolysis reaction on impure protein in the traditional Chinese medicine crude polysaccharide, and the device has the characteristics of simplicity in operation, mild conditions and environment friendliness. The immobilized papain is limited between the two layers of screens, so that the problem of separation of the immobilized papain in the traditional Chinese medicine crude polysaccharide solution can be avoided, and the method has the characteristics of rapidness and convenience. The immobilized papain in the device can be expanded to immobilize other proteases, such as bromelain, subtilisin, protease K and the like, on cellulose according to protein types in different traditional Chinese medicine polysaccharide solutions.

The invention provides a pharmaceutical composition for improving bone mineral density and a preparation method thereof, and belongs to the technical field of biological medicine, the pharmaceutical composition comprises lactarius vomica extract peptide, winged yam rhizome peel extract, rainbow trout extract, calcium citrate-glycine chelate, vitamin K2 and vitamin D3. The lactarius volemus extract peptide is obtained by sequentially carrying out enzymolysis on lactarius volemus by bromelain and subtilisin and purifying and extracting enzymatic hydrolysate through an LH-60 sephadex column. The winged yam rhizome peel extract is obtained by extracting the peels of winged yam rhizome tubers with an ethanol water solution and purifying and extracting the extracting solution with an AB-8 macroporous adsorption resin column. The rainbow trout extract is a product below 5kDa obtained by sequentially performing enzymolysis on fish heads, fish skins and fish scales of rainbow trout through trypsin and lumbrukinase. All the components are compounded according to a specific proportion, and the core effect of improving the bone mineral density is achieved through two-way regulation and control of bone metabolism, optimization of calcium utilization and improvement of safety.

Provided are a small interfering RNA (siRNA) used to inhibit the expression of proprote in convertase subtilisin / kexin type 9 (PCSK9) in a cell, a vector comprising nucleotides encoding same, and a cell, as well as a method using the siRNA, the vector or the cell for the treatment of diseases or symptoms associated with the expression of PCSK9 in a subject.

The invention discloses application of an alpha-amylase / subtilisin inhibitor LASI gene in improvement of drought resistance and salt resistance of crops. The nucleotide sequence of the Ligusticum wallichii LASI gene is as shown in SEQ ID NO. 1; transgenic arabidopsis thaliana plants are obtained through genetic engineering and transgenic identification methods, the drought resistance and the salt resistance of transgenic and wild type arabidopsis thaliana are analyzed through growth indexes and biochemical indexes, and LASI transgenic arabidopsis thaliana with the drought resistance and the salt resistance is successfully obtained. The invention provides a new gene source for cultivating crops with stronger drought resistance and salt tolerance by utilizing a gene engineering technology.

Disclosed herein are one or more subtilisin variants, nucleic acids encoding the same, and compositions and methods relating to the production and use thereof, including one or more subtilisin variants having improved stability and / or soil removal compared to one or more reference subtilisin.

This invention provides an antigen-binding protein that binds to proprotein converterase subtilisin kexin type 9 (PCSK9), as well as a method for using and producing the antigen-binding protein. [Solution] The present invention provides an isolated neutralizing antigen-binding protein that interacts with proprotein converterase subtilisin kexin type 9 (PCSK9), and which contains a specific amino acid sequence that binds to the PCSK9 protein and reduces the LDLR-reducing effect of PCSK9 on LDLR.

Disclosed herein is one or more subtilisin variant, nucleic acid encoding same, and compositions and methods related to the production and use thereof, including one or more subtilisin variant that has improved stability and / or soil removal compared to one or more reference subtilisin.

The present invention belongs to the field of biomedical technologies, and specifically relates to the use of a biomarker in the preparation of a product for diagnosing sepsis. The biomarker is proprotein convertase subtilisin / kexin type 6 (PCSK6), which has significantly high sensitivity and specificity in diagnosing sepsis.

Disclosed herein is a detergent composition comprising a subtilisin variant that has improved stability and / or soil removal compared to one or more reference subtilisin.

Disclosed herein is one or more subtilisin variant, nucleic acid encoding same, and compositions and methods related to the production and use thereof, including one or more subtilisin variant that has improved stability and / or soil removal compared to one or more reference subtilisin.

The present disclosure provides pharmaceutical compositions comprising fibronectin based scaffold domain proteins that bind, for example, proprotein convertase subtilisin kexin-9 (PCSK9).

Disclosed herein is a cleaning composition comprising at least one subtilisin variant and at least one surfactant, and a method for cleaning a surface with said cleaning composition.

Provided herein are gene repressor systems comprising fusion proteins, such as fusion proteins comprising a DNA binding domain such as a TALE, zinc finger or catalytically-dead CRISPR protein and guid

The invention relates to Proprotein Convertase Subtilisin Kexin type 9 (PCSK9) antagonists, such as antibodies and fragments, as well as methods, uses and combinations.

The application provides a preparation method, composition and application of tree orchid flower oil, and relates to the technical field of cigarette flavors. The preparation method of the tree orchid flower oil comprises the following steps: step one, drying and crushing tree orchid flowers to obtain tree orchid flower powder; step two, uniformly mixing the tree orchid flower powder and water, adding enzyme preparation, and performing enzymolysis reaction under the condition that the temperature is 40-50 DEG C and the pH value is 4-7 for 1-5 h to obtain a treatment liquid; the enzyme preparation is composed of hemicellulase, pectinase, xylanase, beta-glucanase and subtilisin with an enzyme activity ratio of (4-6):(3-5):(3-5):(1-3):(1-2); and step three, performing supercritical carbon dioxide fluid extraction on the treatment liquid to obtain tree orchid flower oil. The application provides tree orchid flower oil suitable for cigarettes, the tree orchid flower oil has high extraction rate, obvious flower fragrance and tea fragrance, no bitter and astringent traditional Chinese medicine smell, and no miscellaneous smell.

The invention belongs to the technical field of protein peptide powder. The invention provides a preparation method of ovalbumin peptide powder, which comprises the following steps: S1, transferring protein liquid into a fermentation tank, adjusting the pH value to 6.0-6.9, adding dry yeast, stirring and fermenting to obtain fermentation liquid; s2, transferring the fermentation liquor obtained in the S1 into an enzymolysis tank, heating to 50-55 DEG C, adding a compound enzyme preparation, carrying out enzymolysis for 7-10 hours, and stirring for 10 minutes per hour to obtain enzymatic hydrolysate; the compound enzyme preparation comprises papain, subtilisin, trypsin and aminopeptidase; and S3, transferring the enzymatic hydrolysate obtained in S2 back to a fermentation tank, cooling, adding dry yeast, stirring, fermenting, spray-drying, and placing in a hot chamber for 3-7 days to obtain the product. According to the ovalbumin peptide powder, the oxidation resistance and the heat resistance are improved at the same time, so that the ovalbumin peptide powder with more excellent functionality and wider application range is obtained.

The present invention relates to a dual-targeting siRNA agent, which comprises two different siRNAs targeting two different genes or pharmaceutically acceptable salts thereof, and the two different siRNAs or salts thereof are linked by a pharmaceutically acceptable ligand. The siRNA is a dsRNA composed of a sense strand and an antisense strand. The two different genes are selected from two of angiotensinogen (AGT), proprotein convertase subtilisin / kexin type 9 (PCSK9), and angiopoietin-like protein 3 (ANGPTL3). The present invention provides the application of the dual-targeting siRNA agent in the preparation of a drug for preventing or treating diseases related to hypertension or / and lipid disorders. The dual-targeting siRNA agent of the present invention can effectively inhibit the expression of two target genes in vivo simultaneously, and has the advantages of strong non-antagonistic activity and high safety. The present invention also provides siRNAs corresponding to the genes of the above dual-targeting siRNA agent and their use for preventing or treating diseases related to hypertension or / and lipid disorders.

The invention relates to the field of bioengineering, in particular to a perch collagen peptide as well as a preparation method and application thereof. The invention provides a preparation method of perch collagen, which comprises the following steps: carrying out acid extraction on pretreated perch scales, hydrolyzing with a first enzyme, filtering, salting out filtrate, centrifuging, collecting precipitate, redissolving, purifying, hydrolyzing with a second enzyme, and heating to obtain the perch collagen, enzymes adopted by the first enzyme hydrolysis and the second enzyme hydrolysis can be the same or different; the enzyme comprises one or more of alkaline protease, papain, subtilisin and pepsin. The collagen peptide extracted from sea bass scales and processed into the sea bass scales is systematically researched and developed, and the collagen peptide has an excellent anti-photoaging effect and high replicability. The collagen peptide is low in molecular weight, high in oxidation resistance and wide in application range, and can be applied to the fields of cosmetics, oral collagen supplements and the like.

The present disclosure provides AprL-clade protease enzymes, including variant AprL-clade protease enzymes, nucleic acids encoding same, and compositions and methods related to the production and use thereof, including an AprL-clade variant subtilisin enzyme that has improved stability and / or soil removal compared to a parent AprL-clade subtilisin enzyme.

The present invention relates to methods of treating or preventing cholesterol related disorders, such as hypercholesterolemia, hyperlipidemia or dyslipidemia, using antibodies against proprotein convertase subtilisin / kexin type 9 (PCSK9). Formulations and methods of producing said formulations are also described.

The present invention relates to a dual-targeting siRNA agent comprising two distinct siRNAs targeting two different genes or their pharmaceutically acceptable salts, wherein the two distinct siRNAs or their salts are linked by a pharmaceutically acceptable ligand. The siRNA is a dsRNA composed of a sense strand and an antisense strand, and the two different genes are selected from a group consisting of angiotensinogen (AGT), proprotein convertase subtilisin / kexin type 9 (PCSK9), and human angiopoietin-like protein 3 (ANGPTL3). The present invention provides the application of the dual-targeting siRNA agent in the preparation of drugs for preventing or treating diseases associated with hypertension and / or dyslipidemia. The dual-targeting siRNA agent described in the present invention can effectively inhibit the expression of two target genes simultaneously in vivo, offering the advantages of strong non-antagonistic activity and high safety. The present invention also provides the siRNAs targeting corresponding genes for the aforementioned dual-targeting siRNA agent and their use for preventing or treating diseases associated with hypertension and / or dyslipidemia.