430 results about "Trypsin" patented technology

The invention relates to a stable silk fibroin liquid condensation body, gel and a preparation method and application thereof, and belongs to the technical field of silk fibroin. The preparation method comprises the following steps: S1, dissolving silk fibroin in a lithium bromide solution, and then adding trypsin for incubation to obtain an enzyme-digested silk fibroin solution; and S2, performing low-temperature programmed centrifugal treatment including an induction stage, a nucleation stage and a liquid-liquid phase separation stage on the enzyme-digested silk fibroin solution, and cleaning to obtain the stable silk fibroin liquid-state aggregate, the method can reduce aggregate liquid drop aggregation and improve the particle size uniformity of the liquid drops.

The invention discloses camel milk polypeptide with xanthine oxidase inhibitory activity and application thereof.The camel milk polypeptide is prepared by taking fresh camel milk as a raw material, conducting low-temperature centrifugation to remove fat, conducting triple enzymolysis through papain, pepsin and trypsin to obtain enzymatic hydrolysate, enabling the enzymatic hydrolysate to pass through an ultrafiltration membrane (the molecular weight cutoff is 10 kDa) to obtain filtrate, and conducting freeze-drying to obtain the camel milk polypeptide. The compound has xanthine oxidase inhibitory activity. The invention also discloses a small molecule polypeptide with xanthine oxidase inhibitory activity. The filtered solution is screened and combined with polypeptides by adopting a xanthine oxidase affinity ultrafiltration method, the activity of the polypeptides is verified through molecular docking and in-vitro enzyme inhibition, four small-molecule polypeptides with xanthine oxidase inhibitory activity are obtained, the sequence of the small-molecule polypeptides is shown as any one of SEQ ID NO.1-4, and the small-molecule polypeptides can be used for preparing drugs with the effect of inhibiting the uric acid level.

The invention relates to a cell collection method capable of simultaneously collecting three cells in a co-culture model, and belongs to the technical field of biology. The invention provides a cell collection method capable of simultaneously collecting three cells in a co-culture model, and the cell collection method comprises the following steps: after a three-cell co-culture model is constructed, taking out a Transwell chamber, retaining cells in the lower chamber, and collecting the cells in the lower chamber; respectively digesting the cells on the two sides of the Transwell cell membrane by using a trypsin solution with the concentration of 0.5 g / 100mL so as to respectively collect the cells on the two sides of the Transwell cell membrane. According to the cell collection method disclosed by the invention, the three cells in the three-cell co-culture model are simultaneously collected in a manner of digesting the cells on the two sides of the Transwell membrane step by step by using pancreatin, so that not only is the cell and consumable cost saved, but also the experimental synchronism of the three cells is ensured, and convenience is provided for optimizing the experimental process.

The invention discloses uniform-particle-size silk fibroin microspheres and a preparation method and application thereof.The preparation method comprises the following steps that a regenerated silk fibroin solution is taken and subjected to trypsin digestion to obtain modified silk fibroin sediment, and the modified silk fibroin sediment is dissolved in an aqueous solution of a beta-folding inhibitor to obtain a silk fibroin solution serving as a dispersed phase; emulsifying through an external pressure type membrane emulsifier to obtain an emulsion containing the silk fibroin emulsion droplets; adding a cross-linking agent into the emulsion to carry out cross-linking reaction, and then adding a crystallization inducer to induce crystallization to obtain primary silk fibroin microspheres; and performing vacuum freeze drying to obtain the silk fibroin microspheres. Silk fibroin is subjected to enzyme digestion modification, silk fibroin fragments with high beta-folding content are reserved, and high structural stability is achieved; the crystallinity of the microspheres is improved by adopting a membrane emulsification mode and combining a multiple crystallization induction method, and the silk fibroin microspheres with uniform particle size and smooth surfaces are obtained.

The invention discloses a corn peptide for dispelling effects of alcohol and protecting liver as well as a preparation method and application thereof, and belongs to the technical field of biological peptide extraction. The preparation method comprises the following steps: crushing and sieving corn, dissolving the corn in deionized water, adjusting the pH value to be alkaline, and performing ultrasonic-assisted extraction to obtain corn homogenate; centrifuging, taking supernate, adjusting the pH to be neutral, centrifuging, adding an ethanol solution with the mass fraction of 10%, standing, carrying out continuous flow centrifugation, suspending precipitates in distilled water, dialyzing in a dialysis bag, and freeze-drying to obtain crude corn protein; the preparation method comprises the following steps: preparing an aqueous solution, carrying out variable-temperature treatment, adjusting the pH value to be alkaline, adding trypsin and alkaline protease, carrying out enzymolysis, repeating the enzymolysis twice, and after the enzymolysis is finished, carrying out heating enzyme deactivation, centrifugation, sediment discarding, ultrafiltration, centrifugation, supernatant taking and spray drying to obtain the corn peptide. The corn peptide can remarkably shorten the drunkenness time and sobering time of mice and reduce the drunkenness duration, and has good effects of dispelling the effects of alcohol and protecting the liver.

The invention discloses modified hydroxytyrosol as well as a preparation method and application thereof. The provided preparation method of the modified hydroxytyrosol comprises the following steps: in the presence of an acidic solvent, mixing hydroxytyrosol, protease and collagen for reaction, mixing a reaction product with an extraction agent, performing centrifugal layering, and collecting upper-layer components, thereby obtaining the modified hydroxytyrosol, the protease comprises one or two of neutral protease and trypsin. Under the enzymolysis action of protease, the collagen can be decomposed into amino acid monomers such as glycine, proline and hydroxyproline, and the amino acid monomers can be subjected to graft modification with hydroxytyrosol; meanwhile, collagen fragments which are not subjected to enzymolysis also exist, and are subjected to graft modification with hydroxytyrosol in the form of collagen characteristic fragments. The oxidation resistance and the stability of the obtained modified hydroxytyrosol are obviously improved compared with those of unmodified hydroxytyrosol.

The invention relates to the technical field of cell engineering, and particularly discloses a construction method and application of a sika deer immortalized renal epithelial cell line, and the construction method comprises the following steps: taking renal epithelial primary cells from healthy sika deer renal cortex tissues to obtain primary renal epithelial cells; treating the primary renal epithelial cells with trypsin to obtain a cell suspension; inoculating the cell suspension into a complete culture medium, and transfecting with lentivirus loaded with SV40 large T antigen genes to obtain transfected cells; 3 [mu] g / mL puromycin is applied to the transfected cells for selective culture, so that non-transfected cells are eliminated, and drug-resistant cells are obtained; carrying out continuous passage on the drug-resistant cells for at least 30 generations to obtain the immortalized renal epithelial cell line of the sika deer; according to the invention, through a specific action mechanism (inhibiting a p53 / pRb pathway and blocking cell cycle exit) of the SV40 large T antigen, a proliferation limit caused by inhibition of telomerase activity of primary cells of the cervidae animals is overcome, and a cell resource library capable of realizing continuous passage is established.

The invention discloses a preparation method of micromolecular active collagen peptide, which comprises the following steps: (1) cutting fresh leather into fragments, pretreating the leather with a sodium bicarbonate solution, rinsing the leather with deionized water, and refrigerating and storing; (2) mixing papain, bromelain, pancreatin, pepsase, alkaline protease, neutral protease and flavourzyme to obtain a compound enzyme preparation; (3) performing high-pressure steam sterilization on the leather at 110-140 kPa and 120-122 DEG C to obtain leather with the hydrolysis degree of 75-85%, then adding distilled water into the leather to obtain mucilage, then adding a compound enzyme preparation into the mucilage, reacting for 5.5-6.5 hours at the pH of 6.8-7.2 and the temperature of 50-55 DEG C, and then performing enzyme inactivation at the temperature of 85-95 DEG C; and (4) after the reaction system is cooled to room temperature, purifying and centrifuging the reaction system, and taking clarified supernate, thereby obtaining the product. Different animal skins are treated, the proportion of peptide fragments with the molecular weight smaller than 1000 Da of the prepared small-molecule active collagen peptide is larger than or equal to 80%, and the hydroxyproline content is larger than 3%.

This invention relates to the field of chicken gizzard lining processing technology, and particularly to an active polypeptide of chicken gizzard lining, its preparation method, and functional products. The preparation method includes the following steps: mixing chicken gizzard lining powder with water and subjecting it to alkaline heat treatment to form a slurry; sequentially adding Bacillus licheniformis alkaline protease, trypsin, and pepsin to the slurry for three separate hydrolysis processes to obtain a hydrolysate; after enzyme inactivation treatment of the hydrolysate, adding Lactobacillus plantarum for fermentation to obtain a fermentation broth; sterilizing the fermentation broth and then filtering it to obtain a fermentation supernatant; sequentially subjecting the fermentation supernatant to membrane filtration, sterilization, and spray drying to obtain the active polypeptide of chicken gizzard lining. The active polypeptide of chicken gizzard lining prepared by the method of this invention exhibits alcohol dehydrogenase activating activity, AGEs inhibitory activity, albumin denaturation inhibitory activity, lipoxygenase inhibitory activity, pancreatic lipase inhibitory activity, and a beneficial bacteria proliferation promoting effect.

The invention relates to the technical field of collagen extraction, and provides a collagen extraction process for increasing hydroxyproline content, which sequentially comprises the steps of raw material pretreatment, deliming and degreasing, directional enzymolysis, gradient pressure-temperature synergistic extraction, purification, concentration and drying, a composite pretreatment agent containing a proline hydroxylase activator, EDTA-2Na and neutral protease is adopted in the raw material pretreatment; deashing and degreasing are combined with a chelating agent solution and ultrasonic treatment; a compound enzyme solution composed of trypsin, papain and proline hydroxylase is used for directional enzymolysis; a two-stage gradient pressure-temperature cooperation mode is adopted for extraction; purification is realized through combination of gel filtration chromatography and ion exchange chromatography; and finally carrying out freeze concentration and vacuum freeze drying to obtain the product. According to the process, through a multi-step synergistic effect, the content of hydroxyproline in collagen is remarkably increased, the product purity is high, natural functional characteristics are reserved, and the process is suitable for the fields of food, cosmetics, biological medicine and the like.

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus cereus as well as a culture and application thereof. The preservation number of the bacillus cereus AK-LY1 is CCTCC (China Center For Type Culture Collection) NO: M 2025756. The bacillus cereus AK-LY1 disclosed by the invention is excellent in trypsin resistance, good in cholate resistance, acid resistance and high temperature resistance, good in inhibition performance on antibiotics such as strong penicillin and chloramphenicol, and relatively good in adhesion capacity in intestinal tracts, and provides a relatively good microecological preparation reference basis for expanding a bacillus cereus resource library and applying the bacillus cereus.

Among other things, the present disclosure provides designed oligonucleotides and compositions thereof. In some embodiments, oligonucleotides and compositions of the present disclosure can provide high levels of adenosine editing. In some embodiments, oligonucleotides and compositions of the present disclosure are useful for treating various conditions, disorders or diseases, e.g., alpha-1 antitrypsin deficiency. In some embodiments, the present disclosure provides methods for treating various conditions, disorders or diseases that can benefit from adenosine editing.

The present invention relates to a cartilage tissue single cell extraction kit and an extraction method thereof, the kit comprises: (1) a first enzyme for enzymatic hydrolysis, the first enzyme for enzymatic hydrolysis comprising one or more of trypsin, chondroitinase ABC and fucosidase; (2) a second enzyme for enzymolysis, wherein the second enzyme for enzymolysis comprises collagenase II, neutral protease II, hyaluronidase and Dnase I; and (3) a basic solution, wherein the basic solution comprises a basic culture medium and / or a buffer solution. The invention also provides a method for extracting single cells from cartilage tissues by using the kit. The cartilage tissue single-cell suspension prepared by the method has the advantages of high cell yield, high activity and the like, and has a wide application prospect.

The invention belongs to the technical field of health-care food, and particularly relates to functional milk assisting in lowering blood pressure and a preparation method thereof.The functional milk comprises, by weight, 99.5% of raw milk and 0.5% of a potassium ion bridged citrus fiber-green tea polyphenol network, raw milk is sequentially subjected to trypsin and proline specific incision enzyme treatment, HPCD-ultrasonic treatment and pulsed magnetic field treatment. The invention provides a systematic solution, which exceeds a simple extraction and addition mode in the prior art, and solves the stability problem of the antihypertensive active component in the processing and storage process.

The invention discloses a gelatin microcarrier lysate as well as a preparation method and application thereof. The gelatin microcarrier lysis buffer comprises lyase, a cell protective agent, a cell energy metabolism protective agent, an antioxidant, a buffer solution and a chelating agent. The gelatin microcarrier lysate has extremely strong protectiveness to cells, abandons damaging trypsin and EDTA, and innovatively adds a cell protection combination of a cell protective agent L-arginine, a cell energy metabolism protective agent sodium pyruvate and an antioxidant vitamin C, so that the cell survival rate (gt; 98%) and the retention rate (gt; the method is high in cell yield, good in safety and wide in range.

Compositions and methods for introducing double-stranded breaks within the SERPINA1 gene are provided. Compositions and methods for reducing and eliminating mutant forms of α1-antitrypsin (AAT), such as seen in subjects having α1-antitrypsin deficiency (AATD), are provided.

The invention discloses a preparation method of composite active peptide with anti-wrinkle, whitening and freckle-removing effects from marine fish byproducts, cod byproducts, namely fish heads, fish bones and fish skins, are used as raw materials and are mixed according to the mass ratio of 3: 3: 4, and the composite active peptide is prepared through the steps of pretreatment, enzymolysis, enzyme deactivation, centrifugal separation and purification. A compound enzyme used in the enzymolysis step is composed of trypsin and papain according to a certain proportion, and the enzyme addition amount, the enzymolysis time, the enzymolysis temperature and other conditions are optimized. The prepared composite active peptide with the anti-wrinkle, whitening and freckle-removing effects from the marine fish by-product has good anti-wrinkle, whitening and freckle-removing effects, resource utilization of the cod by-product is achieved, and the composite active peptide has high application value.

The invention relates to the technical field of microcapsule preparation, and particularly discloses a microcapsule composition with a slow release function and a preparation method and application of the microcapsule composition. Soybean protein provided by the invention is subjected to three-stage hydrolysis to generate small molecule peptide and amino acid, and the small molecule peptide and amino acid can be interwoven with maltodextrin in a network structure; therefore, a compact and coherent composite wall material network structure is jointly constructed, erosion of gastric acid can be effectively resisted, and physical isolation protection is provided for probiotics. When the microcapsule enters an environment from neutral to alkalescent intestinal tracts, the pH value in the intestinal tracts is increased, and an original hydrogen bond network structure among molecules is damaged, so that an original compact gel network structure becomes loose and is greatly swelled; meanwhile, the hydrolyzed soybean protein can be further hydrolyzed under the action of intestinal trypsin and the like, the wall material structure of the microcapsule is gradually loosened, thalli are gradually released in the swelling-disintegration process, targeted slow release of the intestinal tract is achieved, and therefore the probiotic function of the microcapsule is achieved.

The invention belongs to the technical field of food, and provides a special enzyme composition for infant soybean milk powder and soybean milk powder thereof. A three-stage directional enzymolysis technology is adopted, the three-stage directional enzymolysis technology comprises glutamine transaminase deamidation pretreatment, bacillus subtilis protease-trypsin-papain first-stage hydrolysis and aminopeptidase-phytase-beta-glucosidase second-stage hydrolysis, and conductivity control, carbon dioxide ventilation in-situ complexation and spray drying processes are combined. The free amino nitrogen of the product is increased by more than or equal to 20%, the proportion of 200-1500Da peptide fragments is more than or equal to 70%, the median particle size is 50-180 microns, the water activity is less than or equal to 0.30, the instant dissolving time is less than or equal to 30 seconds at 25 DEG C under the water hardness of 15-200ppm, the settling rate is less than or equal to 3% after standing for 30 minutes, and the zeta potential is-10 to-25mV. The palatability contradiction between deep hydrolysis and bitterness inhibition of the bean-based infant formula powder, the brewing contradiction between high-calcium reinforcement and instant stability and the process controllability contradiction between three-stage enzymolysis and ionic strength accumulation are effectively solved, and the bean-based infant formula powder has wide application value in the fields of bean-based infant formula food and vegetable protein nutritional products.

The invention discloses a macrobrachium rosenbergii probiotic biofloc technology (BFT) culture system construction method based on an SBOS-FOS composite carbon source, and belongs to the technical field of aquaculture. The total ratio of SBOS (soybean oligosaccharide) and FOS (fructo-oligosaccharide) in the composite carbon source is 2%-10%, and the balance is glucose; and adding the composite carbon source into a macrobrachium rosenbergii culture system according to the C / N ratio of (10: 1)-(20: 1) according to the feed protein content in the culture water body. Through a specific ratio of SBOS to FOS, the content of functional probiotics in a culture water body is specifically increased, stable control of ammonia nitrogen and nitrite nitrogen and directional optimization of a carbon source-flora-host three-level regulation and control network in a BFT system are realized, the weight gain rate (WG), the specific growth rate (SGR) and digestive enzyme activity (trypsin and amylase) of macrobrachium rosenbergii are increased, and the survival rate of the macrobrachium rosenbergii is increased. The abundance of probiotics in the water body and the immune gene expression of the macrobrachium rosenbergii are obviously improved.

The invention discloses a multi-enzyme compound noodle stretching agent and application thereof, and belongs to the technical field of food additives. The multi-enzyme compound noodle stretching agent is prepared from the following components in parts by mass: 1 to 10 parts of papain, 1 to 10 parts of trypsin, 1 to 10 parts of glucose oxidase, 1 to 10 parts of lipase and 10 to 30 parts of tea polyphenol. Through the synergistic effect of multienzyme compounding and tea polyphenol, the dough structure and the noodle processing performance are improved, the protein depolymerization and crosslinking degree and the protein oxidation degree of the dough can be regulated and controlled at the same time, the dough strength and ductility are considered, the cooking loss is reduced, meanwhile, the green and natural characteristics are kept, no chemical modifier is added, and the production cost is reduced. And the limitation of the existing single enzyme or chemical modifier is overcome, so that the method has a relatively good application prospect in the technical field of food additives.

The invention discloses a preparation method of a crocodile polypeptide extract and a beverage, and relates to the technical field of polypeptide extraction and beverage preparation, and the preparation method comprises the following steps: pretreatment of crocodile skin; denaturation treatment: adding a NaHCO3 buffer solution, and treating under a microwave condition; performing ultrasonic pretreatment; carrying out enzymolysis reaction, namely sequentially carrying out primary enzymolysis of alkaline protease and secondary enzymolysis of compounding trypsin and neutral protease; adding composite colloid after enzyme deactivation treatment, wherein the composite colloid comprises flaxseed gum and xanthan gum; centrifuging to take supernate, and carrying out fractional separation through ultrafiltration membranes with the molecular weight cut-off of 500Da and 1000Da; and concentrating and drying to obtain polypeptide powder. According to the method, the polypeptide yield, the beverage stability and the oral bioavailability are improved; finally, the obtained polypeptide is scientifically matched with hyaluronic acid and vitamin C to prepare the beautifying beverage with the effects of remarkably improving skin stains and fine wrinkles and brightening the skin color, and the breakthrough of high-value utilization of crocodile skin resources and product function integration is realized.

The invention relates to a low-temperature gradient enzymolysis technology for collagen peptide in the body wall of a sea cucumber, and discloses mild decomposition and efficient extraction of collagen in the body wall of the sea cucumber by virtue of a multi-section temperature zone reaction, enzyme seed compounding control and on-line regulation system. And the collagen peptide with concentrated molecular weight, strong biological activity and high terminal product quality is prepared by the low-temperature gradient enzymolysis technology. The method is characterized by comprising the following steps: cutting the body wall of the sea cucumber to obtain a particle size suitable for enzymolysis, adding the cut body wall of the sea cucumber into a neutral buffer solution, stirring at normal temperature, and adjusting the pH value to 6.5-7.5, so that the tissue of the body wall of the sea cucumber is fully expanded and uniformly dispersed to be more suitable for the subsequent enzymolysis process; the method comprises the following steps: carrying out staged reaction on the body wall of the sea cucumber and an enzyme at different temperatures, carrying out fine separation and terminal treatment on an enzymatic hydrolysate, and immediately cooling a system to 4 DEG C after the reaction of the enzymatic hydrolysate is finished.

The invention provides an anti-aging and skin-tightening composition and a preparation method thereof, and belongs to the technical field of anti-aging and skincare, and the composition comprises a hovenia acerba infructescence extract, an okra seed extract, tetrapeptide-4, acetyl octapeptide-1, a hill-pit snail extract, paeonol and a carnosine-zinc gluconate chelate. And carrying out aerobic fermentation on the raisin tree infructescence powder through aspergillus oryzae, and purifying the centrifuged supernatant through a composite filler resin column to obtain the raisin tree infructescence extract. The preparation method comprises the following steps: sequentially carrying out enzymolysis on okra seeds by virtue of compound enzyme, ficin and subtilisin, adding absolute ethyl alcohol for extraction, centrifuging supernate, and purifying by virtue of an LH-20 sephadex column, so as to obtain the okra seed extract. The preparation method comprises the following steps: sequentially carrying out enzymolysis on the mountain snail meat through trypsin and lumbrukinase, centrifuging the enzymatic hydrolysate, and carrying out ultrafiltration on the supernate to obtain the mountain snail extract with the molecular weight of less than 3kDa. The components are matched for use, so that the balance of high-efficiency anti-aging and low-irritation can be realized through synergism of three major pathways of inhibiting collagen degradation, promoting collagen synthesis and resisting sugar and relieving.

The invention provides a wheat gliadin detection method, and belongs to the technical field of biology. The detection method comprises the following steps: mixing a sample to be detected with an ethanol solution to obtain a mixture; performing water bath on the mixture to obtain a mixed extract; centrifuging the mixed extract to obtain a supernatant; adding a reductive alkylation reagent into the supernate to obtain a reaction product; adding alkaline trypsin into the reaction product to carry out enzymolysis reaction to obtain an enzymolysis product; a first characteristic peptide fragment and a second characteristic peptide fragment as shown in SEQ ID NO: 1 in the sequence table are respectively used as markers, and the wheat gliadin is measured by the enzymolysis product through ultra-high performance liquid chromatography-tandem mass spectrometry by utilizing the markers. The method is simple in process and capable of efficiently detecting the wheat gliadin.

The application discloses a fabric yellowing reagent and a fabric yellowing method, and relates to the technical field of daily chemical products. 5 The fabric yellowing reagent comprises 1-3% of histidine solution and a bacterial suspension with a bacterial concentration of 1*10 5 CFU / mL-9*10 The bacteria are a mixture of one or more of Staphylococcus epidermidis and Micrococcus luteus; the solvent of the histidine solution is normal saline containing 0.15-1% of trypsin peptone; and the solvent of the bacterial suspension is normal saline containing 0.1-0.2% of trypsin soybean peptone liquid culture medium. The fabric yellowing reagent can be used for simulating fabric yellowing, and has good operability, reproducibility and data reliability.

The invention belongs to the technical field of protein, and relates to an anti-photoaging damage peptide and a preparation method thereof. The amino acid sequence of the peptide is KSYELPDGQVITI, and the key active fragment of the peptide is ELPDGQVIT. The peptide is obtained by performing enzymolysis on degreased maggot powder with alkaline protease, trypsin, papain, neutral protease and flavourzyme. The peptide can remove ROS free radicals after UVA irradiation, improve SOD activity and inhibit MDA generation, and finally alleviate oxidative stress caused by UVA.

The invention belongs to the technical field of protein peptide preparation, and particularly relates to wheat oligopeptide with an anti-fatigue effect and a preparation method of the wheat oligopeptide. According to the method, the raw materials are subjected to enzymolysis by using the first compound enzyme containing bacillus subtilis protease and bromelain and the second compound enzyme containing trypsin, flavourzyme and papain step by step, and treatment of centrifugation, ultrafiltration and the like is combined, so that the yield and the taste of the product are improved, and the proportion of small molecular weight peptide in the product is high. Therefore, the wheat oligopeptide prepared by the method is high in yield and good in taste, not only is beneficial to improving the endurance of the body and prolonging the exercise time, but also can improve the energy metabolism condition in the body, delay the accumulation of lactic acid, urea nitrogen and creatine kinase in the body after exercise and increase the glycogen storage amount, and macroscopically shows certain anti-fatigue ability. The technical scheme of the invention has strong practicability, is beneficial to industrial production, and can be applied to preparation of anti-fatigue functional food.

The invention relates to a fermentation enzymolysis technology, in particular to a production method for optimizing natural cheese essence by utilizing an electric field auxiliary technology. The method comprises the following steps: preheating milk, adjusting the pH value, adding trypsin and lipase, and uniformly mixing; and putting the obtained milk containing the enzyme into an electric field assisted reactor, carrying out electric field assisted fermentation enzymolysis under a stirring condition, and carrying out post-treatment on the obtained reaction product to obtain the cheese essence. By combining the electric field technology and the enzymolysis process, the reaction efficiency and the product quality are remarkably improved.

The invention relates to a pharmaceutical composition for repairing oral gingivitis and a preparation method thereof, and belongs to the technical field of biological medicine, the pharmaceutical composition comprises a lactobacillus salivarius fermentation extract, a herba violae extract, golden cicada nymph extract peptide, guava polyphenol and resveratrol; the lactobacillus salivarius fermentation extract is obtained by carrying out aerobic fermentation and anaerobic fermentation on lactobacillus salivarius to obtain a fermentation product, and purifying and extracting the fermentation product through an XAD7HP macroporous adsorption resin column; the philippine violet herb extract is obtained by performing enzymolysis on whole philippine violet herb through pectinase and cellulase and then performing enzymolysis through compound protease to obtain zymolyte, and purifying and extracting the zymolyte through a compound adsorption column. The golden cicada nymph extracted peptide is obtained by purifying and extracting 5kDa zymolyte obtained by performing step-by-step enzymolysis on golden cicada nymphs through papain and trypsin through a Q agarose gel FF adsorption column. All the components are matched for use according to a certain proportion, so that the antibacterial coating is capable of precisely resisting bacteria, has efficient anti-inflammatory and anti-oxidation properties, and is safe and reliable.