947 results about "Trypsin" patented technology

Compositions and methods for editing deleterious mutations associated with Alpha-1 Antitrypsin Deficiency (A1AD). In particular embodiments, the invention provides methods for treating A1AD using a modified adenosine base editor with improved on-target editing and decreased off-target editing to correct mutations associated with A1AD.

The invention relates to a weight-losing enzyme and a preparation method thereof, and belongs to the technical field of biology, and the weight-losing enzyme comprises a fruit enzyme, a vegetable enzyme, a seed enzyme and a lotus leaf extract; the fruit enzyme is obtained by performing enzymolysis on mixed fruit pulp of apples, lemons, grapefruits and haws through pectinase, performing enzymolysis through pepsase and performing co-fermentation through bifidobacterium longum and lactobacillus acidophilus; the vegetable enzyme is obtained by performing enzymolysis on mixed vegetable pulp of wax gourd pulp, celery and spinach through pectinase, performing enzymolysis through pepsin and performing fermentation through lactobacillus plantarum and bifidobacterium adolescentis; the seed enzyme is obtained by carrying out trypsin enzymolysis on mixed powder of semen raphani and phaseolus calcaratus, then carrying out nattokinase enzymolysis and then fermenting with bifidobacterium breve; the lotus leaf extract is obtained by sequentially carrying out enzymolysis on lotus leaves through hemicellulase, snail protease and nattokinase. All the components are matched for use, so that the weight-losing food has the effects of promoting digestion, inhibiting fat absorption, regulating intestinal flora, promoting metabolism and the like, and has a good weight-losing effect.

The invention discloses a method for promoting dissolution of eggshell membrane protein and preparing chelated calcium through high-pressure crushing. Comprising the following steps: separating and drying eggshells and eggshell membranes of egg shells, and respectively carrying out superfine grinding to obtain eggshell powder and eggshell membrane powder; adding water into the eggshell membrane powder, carrying out high-pressure homogenization, and centrifuging to obtain an eggshell membrane solution; adding eggshell powder into the eggshell membrane solution, adjusting the pH value to 1-3, and adding pepsase for reaction; after the reaction, adjusting the pH value of the eggshell membrane solution to 6.5-7.5, and adding trypsin for reaction; and after the reaction, inactivating the enzyme, centrifuging to take a supernatant, adding absolute ethyl alcohol into the supernatant for alcohol precipitation, and centrifuging to obtain a precipitate, namely the chelated calcium. The technical problem that the eggshell membrane protein is difficult to dissolve out is solved, and a new way is provided for industrial extraction of the eggshell membrane soluble protein.

The invention relates to a stable silk fibroin liquid condensation body, gel and a preparation method and application thereof, and belongs to the technical field of silk fibroin. The preparation method comprises the following steps: S1, dissolving silk fibroin in a lithium bromide solution, and then adding trypsin for incubation to obtain an enzyme-digested silk fibroin solution; and S2, performing low-temperature programmed centrifugal treatment including an induction stage, a nucleation stage and a liquid-liquid phase separation stage on the enzyme-digested silk fibroin solution, and cleaning to obtain the stable silk fibroin liquid-state aggregate, the method can reduce aggregate liquid drop aggregation and improve the particle size uniformity of the liquid drops.

The invention belongs to the technical field of biology, and particularly relates to an extraction method of polydeoxyribonucleotide, which comprises the following steps: carrying out homogenate treatment on pretreated in-vitro testis tissue, filtering the obtained homogenate, adding a pancreas extract into the filtrate, and stirring to obtain enzymatic hydrolysate; and precipitating protein in the enzymatic hydrolysate, removing the protein, adding sodium acetate for treatment, adding absolute ethyl alcohol for precipitation, centrifuging, washing the obtained precipitate, and drying to obtain the product. Common trypsin and lipase in fish pancreas are beneficial to release of DNA, the fish pancreas extract is applied to extraction of the polydeoxyribonucleotide, the extraction time can be remarkably shortened, the yield can be increased, meanwhile, nuclease in the fish pancreas extract can effectively degrade DNA, and small-fragment polydeoxyribonucleotide is obtained.

The invention provides a pumpkin seed protein peptide for improving vascular endothelial cell dysfunction and a preparation method thereof, and relates to the technical field of bioactive peptides, the pumpkin seed protein peptide is obtained by enzymolysis of pumpkin seed crude protein with compound protease; the compound protease comprises alkaline protease and trypsin; the mass ratio of the alkaline protease to the trypsin is (1: 3)-(3: 1), and compared with other enzymolysis modes, the pumpkin seed protein peptide prepared by performing enzymolysis on the pumpkin seed crude protein by adopting the alkaline protease and the trypsin has the advantage that the capability of scavenging peroxide free radicals is improved; and the arginine is enriched. The form of peptide in the pumpkin seed protein peptide conforms to the characteristics of high-activity antihypertensive active peptide; the traditional Chinese medicine composition has a relatively good improvement effect on vascular endothelial cell dysfunction and can promote the generation of NO factors. The technical problem that in the prior art, no bioactive peptide capable of improving vascular endothelial cell dysfunction exists is solved.

The disclosure provides compounds useful for treating alpha-1 antitrypsin deficiency (AATD), according to formula (I):tautomers thereof, pharmaceutically acceptable salts of the compounds, pharmaceutically acceptable salts of the tautomers, deuterated derivatives of the compounds, deuterated derivatives of the tautomers, and deuterated derivatives of the salts, solid forms of those compounds and processes for making those compounds.

The invention relates to the field of biological medicine, and provides a preparation method of a fermentation product containing giant salamander peptide, which comprises the steps of stepped enzymolysis pretreatment, staged inoculated fermentation, synergistic enzymolysis and molecular weight grading purification. The method comprises the following steps: firstly, carrying out staged enzymolysis on giant salamander tissues by utilizing pepsin, trypsin and flavourzyme to obtain a primary peptide solution; then, bacillus subtilis, lactobacillus plantarum and bifidobacterium are adopted for directional fermentation, and flora metabolism is optimized through dissolved oxygen control and pH automatic regulation. Then, compound protease is added for secondary enzymolysis, so that the yield of the small molecule peptide is increased. And finally, carrying out ultrafiltration and nanofiltration graded purification, vacuum concentration, freeze drying and gamma-ray sterilization to obtain the micromolecular giant salamander peptide. The molecular weight of the obtained product is mainly distributed within 0.5-5 kDa (lt), and the proportion of 3 kDa is larger than or equal to 80%), the DPPH free radical scavenging rate is larger than or equal to 85%, the ABTS < + > scavenging rate is larger than or equal to 80%, and the obvious anti-oxidation and auxiliary liver protection effects are achieved.

The invention discloses camel milk polypeptide with xanthine oxidase inhibitory activity and application thereof.The camel milk polypeptide is prepared by taking fresh camel milk as a raw material, conducting low-temperature centrifugation to remove fat, conducting triple enzymolysis through papain, pepsin and trypsin to obtain enzymatic hydrolysate, enabling the enzymatic hydrolysate to pass through an ultrafiltration membrane (the molecular weight cutoff is 10 kDa) to obtain filtrate, and conducting freeze-drying to obtain the camel milk polypeptide. The compound has xanthine oxidase inhibitory activity. The invention also discloses a small molecule polypeptide with xanthine oxidase inhibitory activity. The filtered solution is screened and combined with polypeptides by adopting a xanthine oxidase affinity ultrafiltration method, the activity of the polypeptides is verified through molecular docking and in-vitro enzyme inhibition, four small-molecule polypeptides with xanthine oxidase inhibitory activity are obtained, the sequence of the small-molecule polypeptides is shown as any one of SEQ ID NO.1-4, and the small-molecule polypeptides can be used for preparing drugs with the effect of inhibiting the uric acid level.

The invention relates to a cell collection method capable of simultaneously collecting three cells in a co-culture model, and belongs to the technical field of biology. The invention provides a cell collection method capable of simultaneously collecting three cells in a co-culture model, and the cell collection method comprises the following steps: after a three-cell co-culture model is constructed, taking out a Transwell chamber, retaining cells in the lower chamber, and collecting the cells in the lower chamber; respectively digesting the cells on the two sides of the Transwell cell membrane by using a trypsin solution with the concentration of 0.5 g / 100mL so as to respectively collect the cells on the two sides of the Transwell cell membrane. According to the cell collection method disclosed by the invention, the three cells in the three-cell co-culture model are simultaneously collected in a manner of digesting the cells on the two sides of the Transwell membrane step by step by using pancreatin, so that not only is the cell and consumable cost saved, but also the experimental synchronism of the three cells is ensured, and convenience is provided for optimizing the experimental process.

The invention relates to a medicine for repairing skin wounds and a preparation method thereof, and belongs to the technical field of biological medicines.The medicine is prepared from, by mass, 25-30 parts of bee embryo peptide, 8-10 parts of alkannin derivative, 10-15 parts of armillaria mellea polysaccharide, 8-10 parts of inonotus obliquus polysaccharide, 6-8 parts of notoginsenoside and 5-7 parts of astragaloside. The bee fetus peptide is a product which is more than 500Da and is obtained by performing step-by-step enzymolysis on bee larva freeze-dried powder through trypsin and bacillus subtilis neutral protease to obtain enzymatic hydrolysate which is less than 3kDa, performing purification through a G-50 sephadex column, performing ultrafiltration and performing freeze-drying. The alkannin derivative is obtained by reacting alkannin with succinic anhydride and glucosamine-polyethylene glycol. A special method is adopted for preparing the bee embryo peptide and alkannin derivatives, the bee embryo peptide and alkannin derivatives are matched with other components to play a synergistic role, and the effects of high safety, high skin cell proliferation promoting capacity, excellent antibacterial performance and the like are achieved.

The invention provides an organic molecule cytoplasm delivery carrier as well as a preparation method and application thereof, the compound for cytoplasm delivery has the characteristic of fat-soluble aggregation, and can form electrostatic adsorption with small molecule protein goods such as RNase A (13.7 KDa), Trypsin (24KDa) and Saporin (32.8 KDa), medium molecule protein goods such as HRP (40KDa) and macromolecular protein goods such as beta-Gal (430KDa), so that the compound for cytoplasm delivery can be used for preparing a lipid-soluble protein carrier. And furthermore, transmembrane transportation is formed through the strong fat solubility property, so that the protection and intracellular delivery of carried protein molecules are realized. According to the delivery mechanism, cargo molecules can quickly penetrate through cell membranes, and carried protein molecules can enter cytoplasm to exert functions without release operation. Development of the organic molecule cytoplasm delivery carrier is expected to assist potential protein therapies with low bioavailability and off-target toxicity to complete clinical transformation, which is crucial to tumor therapy, gene therapy and the like requiring precise local administration.

The invention discloses uniform-particle-size silk fibroin microspheres and a preparation method and application thereof.The preparation method comprises the following steps that a regenerated silk fibroin solution is taken and subjected to trypsin digestion to obtain modified silk fibroin sediment, and the modified silk fibroin sediment is dissolved in an aqueous solution of a beta-folding inhibitor to obtain a silk fibroin solution serving as a dispersed phase; emulsifying through an external pressure type membrane emulsifier to obtain an emulsion containing the silk fibroin emulsion droplets; adding a cross-linking agent into the emulsion to carry out cross-linking reaction, and then adding a crystallization inducer to induce crystallization to obtain primary silk fibroin microspheres; and performing vacuum freeze drying to obtain the silk fibroin microspheres. Silk fibroin is subjected to enzyme digestion modification, silk fibroin fragments with high beta-folding content are reserved, and high structural stability is achieved; the crystallinity of the microspheres is improved by adopting a membrane emulsification mode and combining a multiple crystallization induction method, and the silk fibroin microspheres with uniform particle size and smooth surfaces are obtained.

The invention provides a genetically engineered bacterium and a construction method of the genetically engineered bacterium, and the genetically engineered bacterium is characterized in that a green fluorescent protein sfGFP is used for mediating protein glutaminase, PG (protein glutaminase), PGF (protein glutaminase), PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF and PGF; eC 3.5. 1.44) is efficiently secreted and expressed in engineering bacteria, and belongs to the technical field of biological engineering. The method comprises the following steps: an obtained sfGFP gene is derived from an NCBI database (GenBank numbering: CP035486.1), an obtained PG zymogen gene PP (Propeptide-Protein glutaminase) is derived from a Chryseobacterium prion prgA gene (GenBank numbering: AB046594.1) in the NCBI database, a recombinant plasmid pHT01 / sfGFP-PP is introduced into bacillus subtilis WB800N to obtain an engineering bacterium, extracellular secretion expression of PP is realized, the sfGFP-PP is activated by trypsin to obtain PG, and the PG is subjected to enzyme activation to obtain the recombinant bacillus subtilis. The expression quantity and the secretion efficiency of the PG in the bacillus subtilis are greatly improved. The recombinant PG produced by the method can generate 23.5 U / mL enzymatic activity under activation of trypsin, can be used for improving protein functional characteristics, and has relatively high industrial production and application values.

The invention relates to a biosynthesis method of cosmetic tripeptide GHK (glycine-histidine-lysine), which is characterized in that a GHK oligopeptide is prepared by carrying out microbial fermentation synthesis and trypsin specific hydrolysis on artificially designed GHK multiple artificial sequences. And on the basis of the original sequence, the GHK yield is improved by adding a specific interval sequence. Compared with a traditional chemical synthesis method, the GHK oligopeptide biosynthesis method has the advantages that raw materials are cheap and simple, steps are easy to implement, and the GHK oligopeptide biosynthesis method is environmentally friendly and beneficial to improving the application level of GHK tripeptide in the field of beauty makeup.

The invention discloses a corn peptide for dispelling effects of alcohol and protecting liver as well as a preparation method and application thereof, and belongs to the technical field of biological peptide extraction. The preparation method comprises the following steps: crushing and sieving corn, dissolving the corn in deionized water, adjusting the pH value to be alkaline, and performing ultrasonic-assisted extraction to obtain corn homogenate; centrifuging, taking supernate, adjusting the pH to be neutral, centrifuging, adding an ethanol solution with the mass fraction of 10%, standing, carrying out continuous flow centrifugation, suspending precipitates in distilled water, dialyzing in a dialysis bag, and freeze-drying to obtain crude corn protein; the preparation method comprises the following steps: preparing an aqueous solution, carrying out variable-temperature treatment, adjusting the pH value to be alkaline, adding trypsin and alkaline protease, carrying out enzymolysis, repeating the enzymolysis twice, and after the enzymolysis is finished, carrying out heating enzyme deactivation, centrifugation, sediment discarding, ultrafiltration, centrifugation, supernatant taking and spray drying to obtain the corn peptide. The corn peptide can remarkably shorten the drunkenness time and sobering time of mice and reduce the drunkenness duration, and has good effects of dispelling the effects of alcohol and protecting the liver.

The invention provides a preparation method, application and composition of oat polypeptide, and relates to the technical field of cosmetics. The preparation method disclosed by the invention comprises the following steps: (1) moistening and swelling oat, drying after steam explosion treatment, and adding pectinase, cellulase and amylase to carry out carbohydrate enzymolysis, so as to obtain oat residues after enzymolysis; (2) adding compound protease into the oat residues subjected to enzymolysis, and carrying out primary proteolysis under the condition that the addition amount of ethanol with the volume concentration of 3-8%, so as to obtain enzymolysis slurry A; the compound protease comprises papain, bromelain and flavourzyme in a mass ratio of (3-6): (3-6): 2; (3) adding trypsin into the enzymolysis serous fluid A to carry out secondary proteolysis to obtain enzymolysis serous fluid B; and (4) carrying out ultrafiltration on the enzymolysis slurry B to obtain the oat polypeptide. The obtained oat polypeptide is small in molecular weight and high in content, is used for preparing cosmetics, and has remarkable effects of preserving moisture, resisting wrinkles and reducing skin roughness.

The present disclosure features useful compositions and methods to treat disorders (e.g., alpha-1-antitrypsin deficiency) for which deamination of an adenosine in an mRNA produces a therapeutic result in a subject in need thereof.

The invention relates to a preparation method of small-molecular-weight sea cucumber oligopeptide, the prepared sea cucumber oligopeptide and application, and belongs to the technical field of skin care products. The preparation method of the small-molecular-weight sea cucumber oligopeptide comprises the following steps: preparing sea cucumber fine powder; adding the sea cucumber fine powder into ultrapure water, adding papain and EDTA (Ethylene Diamine Tetraacetic Acid), and carrying out enzymolysis under the assistance of microwaves; adding trypsin and calcium ions, and carrying out enzymolysis under the assistance of microwaves; adding neutral protease and zinc ions, and performing enzymolysis under the assistance of microwaves to obtain final sea cucumber enzymatic hydrolysate; and decolorizing, separating and purifying, and freeze-drying to obtain the powdery small-molecular-weight sea cucumber oligopeptide. According to the preparation method of the small-molecular-weight sea cucumber oligopeptide, microwave heating and enzymolysis reaction are combined, the hydrolysis degree of a peptide chain is regulated and controlled by controlling an enzyme hydrolysis sequence and enzymolysis conditions, the small-molecular-weight sea cucumber oligopeptide with relatively concentrated and moderate molecular weight is prepared, and good biological activity of the sea cucumber oligopeptide is kept.

The invention discloses modified hydroxytyrosol as well as a preparation method and application thereof. The provided preparation method of the modified hydroxytyrosol comprises the following steps: in the presence of an acidic solvent, mixing hydroxytyrosol, protease and collagen for reaction, mixing a reaction product with an extraction agent, performing centrifugal layering, and collecting upper-layer components, thereby obtaining the modified hydroxytyrosol, the protease comprises one or two of neutral protease and trypsin. Under the enzymolysis action of protease, the collagen can be decomposed into amino acid monomers such as glycine, proline and hydroxyproline, and the amino acid monomers can be subjected to graft modification with hydroxytyrosol; meanwhile, collagen fragments which are not subjected to enzymolysis also exist, and are subjected to graft modification with hydroxytyrosol in the form of collagen characteristic fragments. The oxidation resistance and the stability of the obtained modified hydroxytyrosol are obviously improved compared with those of unmodified hydroxytyrosol.

The invention relates to the technical field of medical care, and particularly discloses a preparation method and application of sea cucumber peptide. The preparation method of the sea cucumber peptide provided by the invention comprises the following steps: heating sea cucumber primary pulp for denaturation, and then adding compound protease for enzymolysis treatment to obtain sea cucumber enzymatic hydrolysate; the addition amount of the compound protease is 0.1-0.15 mg / mL, and the compound protease is prepared from papain, ficin and trypsin according to the weight ratio of 1: (0.5-1.5): (2-3); adding activated carbon and diatomite into the sea cucumber enzymatic hydrolysate, and performing enzyme deactivation, filtration and concentration treatment to obtain sea cucumber peptide; the invention also provides application of the sea cucumber peptide obtained by the preparation method in medicines and foods. According to the preparation method of the sea cucumber peptide, the sea cucumber peptide with small molecular weight and good uniformity can be obtained, and the prepared sea cucumber peptide tablet candy has the effects of relieving physical fatigue, resisting aging, resisting oxidation and the like.

The invention discloses a method for auxiliary screening of grease antioxidant peptides based on artificial intelligence, and belongs to the technical field of bioinformatics. According to the invention, deep learning and virtual enzymolysis are combined, so that a novel method for preparing the antioxidant peptide by rapidly and efficiently screening protein enzymolysis is researched and developed. According to the method, an antioxidant peptide is obtained as a target, an antioxidant peptide data set and a test set are constructed, a Bi-LSTM high-precision deep learning strategy is provided based on the data set, and the Bi-LSTM high-precision deep learning strategy is trained to recognize the antioxidant peptide. And then, predicting the antioxidant activity of the polypeptide obtained through virtual enzymolysis by utilizing Bi-LSTM (Bidirectional Long Short Term Memory). Taking black bean protein as an example, the strategy of the invention discovers that the antioxidant peptide can be better obtained by enzymolysis of the black bean protein through trypsin. After ABTS and DPPH activity verification, the antioxidant peptide is used for lipophilicity determination, the peptide with lipophilicity can play a better grease antioxidant effect, and a research basis is provided for safety control of a grease hot working process.

The invention relates to the technical field of cell engineering, and particularly discloses a construction method and application of a sika deer immortalized renal epithelial cell line, and the construction method comprises the following steps: taking renal epithelial primary cells from healthy sika deer renal cortex tissues to obtain primary renal epithelial cells; treating the primary renal epithelial cells with trypsin to obtain a cell suspension; inoculating the cell suspension into a complete culture medium, and transfecting with lentivirus loaded with SV40 large T antigen genes to obtain transfected cells; 3 [mu] g / mL puromycin is applied to the transfected cells for selective culture, so that non-transfected cells are eliminated, and drug-resistant cells are obtained; carrying out continuous passage on the drug-resistant cells for at least 30 generations to obtain the immortalized renal epithelial cell line of the sika deer; according to the invention, through a specific action mechanism (inhibiting a p53 / pRb pathway and blocking cell cycle exit) of the SV40 large T antigen, a proliferation limit caused by inhibition of telomerase activity of primary cells of the cervidae animals is overcome, and a cell resource library capable of realizing continuous passage is established.

The invention discloses a preparation method and application of an umbilical cord extract for improving and repairing hair follicle miniaturization. The preparation method of the umbilical cord extract comprises the following steps: washing an animal umbilical cord, cutting the animal umbilical cord into pieces, digesting the animal umbilical cord with pancreatin, adding a serum-free mesenchymal stem cell culture medium, blowing and beating to disperse tissues, filtering, performing low-energy red light irradiation on the filtered tissue fluid, incubating for 10-12 hours, adding the serum-free mesenchymal stem cell culture medium with the same volume, continuously incubating for 48-72 hours, and performing freeze drying to obtain the umbilical cord extract. And adding an isometric serum-free mesenchymal stem cell culture medium, adding mir-135b at the same time, treating for 48-72 hours, taking out tissue fluid, performing freeze thawing, ultrasonic treatment and filtration, and diluting filtrate with sterile water to obtain the serum-free mesenchymal stem cell culture medium. The umbilical cord extract can remarkably improve and repair miniaturized hair follicles and promote the diameter of hair to recover to normal, can be used as a novel raw material, and is used for preparing cosmetics and medicines with the effects of improving and repairing the miniaturized hair follicles, promoting hair growth or preventing alopecia.

The invention discloses a method for extracting micro-plastics in soil of a refuse landfill. The method comprises the following steps: pretreating a refuse landfill soil sample to obtain sample soil; the sample soil and an extracting agent are fully mixed and oscillated and then stand for layering, first supernate is obtained, the extracting agent is added at least twice, the types of the extracting agent added each time are different, and the extracting agent added each time is one of saturated sodium chloride, saturated sodium bromide and saturated sodium iodide; the first supernate is extracted and fully mixed with a digesting agent, the mixture is shaken and then stands for layering again, second supernate is obtained, and the digesting agent is at least one of 30% hydrogen peroxide, a Fenton reagent, pepsin and trypsin; and filtering the second supernatant to obtain the micro-plastic. Therefore, the extraction method provided by the embodiment of the invention has multiple technical effects of high efficiency, deep digestion, accurate filtration, wide applicability, environmental friendliness and the like.

The invention discloses a preparation method of elastin peptide, and relates to the technical field of natural elastin extraction.The preparation method comprises the following steps that S1, animal blood vessels are cleaned, subjected to impurity removal, cut into blocks and minced; s2, the diced and minced animal blood vessels are soaked in alkali liquor for degreasing, the animal blood vessels are fished out and cleaned after degreasing, water is added after cleaning, pulping is conducted, slurry is obtained, and the pH of the slurry is adjusted to 7.5-8.5; s3, adding elastinase and trypsin into the pulp, and performing enzymolysis to obtain enzymatic hydrolysate; s4, sequentially performing high-temperature enzyme deactivation, vacuum concentration, purification, refining and spray drying on the enzymatic hydrolysate to obtain powdery elastin peptide. The trypsin and elastinase adopted in the invention belong to basic proteins, can decompose proteins under alkaline conditions, and have the characteristics of high catalysis efficiency, action specificity, activity adjustability, diversity and the like.

The invention discloses a preparation method of micromolecular active collagen peptide, which comprises the following steps: (1) cutting fresh leather into fragments, pretreating the leather with a sodium bicarbonate solution, rinsing the leather with deionized water, and refrigerating and storing; (2) mixing papain, bromelain, pancreatin, pepsase, alkaline protease, neutral protease and flavourzyme to obtain a compound enzyme preparation; (3) performing high-pressure steam sterilization on the leather at 110-140 kPa and 120-122 DEG C to obtain leather with the hydrolysis degree of 75-85%, then adding distilled water into the leather to obtain mucilage, then adding a compound enzyme preparation into the mucilage, reacting for 5.5-6.5 hours at the pH of 6.8-7.2 and the temperature of 50-55 DEG C, and then performing enzyme inactivation at the temperature of 85-95 DEG C; and (4) after the reaction system is cooled to room temperature, purifying and centrifuging the reaction system, and taking clarified supernate, thereby obtaining the product. Different animal skins are treated, the proportion of peptide fragments with the molecular weight smaller than 1000 Da of the prepared small-molecule active collagen peptide is larger than or equal to 80%, and the hydroxyproline content is larger than 3%.

The invention relates to a skin wound repairing material and a preparation method thereof, and belongs to the technical field of biological medicines. The repair material disclosed by the invention contains palmitoyl tripeptide-1, methacrylated collagen, cuttlebone extract, recombinant human epidermal growth factor lipidosome, polydopamine nanoparticles, hyaluronic acid, sodium alginate modified silk fibroin and the like in a specific proportion. Wherein the cuttlebone extract is a product which is obtained by carrying out common enzymolysis on cuttlebone through chitinase and nattokinase and then carrying out step-by-step enzymolysis on the cuttlebone through gelatinase and lumbrukinase and is less than 5kDa. The components are prepared into an efficient product by adopting a special method, the stability of the components is improved, the problem that the activity is difficult to maintain is solved, and the skin wound repairing effect is good through reasonable compatibility.

The present invention relates to a prebiotic feed additive for promoting intestinal health of pigs, belonging to the technical field of feed, the prebiotic feed additive of the present invention comprises prebiotic components and biological adjuvants, the prebiotic components comprise oligofructose, oligoisomaltose, inulin and resistant dextrin, the biological adjuvant is a product obtained by co-enzymatic hydrolysis of dandelion and houttuynia cordata by hemicellulase and pectinase, then fermented by Lactobacillus acidophilus and Propionibacterium freudenreichii in sequence, and finally enzymatic hydrolyzed by bromelain and trypsin step by step. The components in the additive are used in combination to have a good effect of promoting intestinal health of pigs, can balance and improve the intestinal microenvironment, improve feed nutrient utilization, enhance immunity, reduce diarrheal diseases, and promote growth and weight gain.

This invention relates to the field of chicken gizzard lining processing technology, and particularly to an active polypeptide of chicken gizzard lining, its preparation method, and functional products. The preparation method includes the following steps: mixing chicken gizzard lining powder with water and subjecting it to alkaline heat treatment to form a slurry; sequentially adding Bacillus licheniformis alkaline protease, trypsin, and pepsin to the slurry for three separate hydrolysis processes to obtain a hydrolysate; after enzyme inactivation treatment of the hydrolysate, adding Lactobacillus plantarum for fermentation to obtain a fermentation broth; sterilizing the fermentation broth and then filtering it to obtain a fermentation supernatant; sequentially subjecting the fermentation supernatant to membrane filtration, sterilization, and spray drying to obtain the active polypeptide of chicken gizzard lining. The active polypeptide of chicken gizzard lining prepared by the method of this invention exhibits alcohol dehydrogenase activating activity, AGEs inhibitory activity, albumin denaturation inhibitory activity, lipoxygenase inhibitory activity, pancreatic lipase inhibitory activity, and a beneficial bacteria proliferation promoting effect.