277 results about "High survival rate" patented technology

The invention relates to an artificial hybridization fry breeding method for epinephelus fuscoguttatus and pink pink bass, which belongs to the technical field of distant hybridization breeding and comprises the steps of parent selection of epinephelus fuscoguttatus and pink pink bass, parent breeding, parent intensive breeding, gamete collection, artificial insemination, hatching of fertilized eggs and fry breeding. By means of reproductive regulation and distant hybridization technologies, two species belonging to two genera under the grouper family with different geographical distribution and different breeding time are hybridized, namely cross-generic hybridization under the grouper family is achieved, intergeneric reproductive isolation is broken through, and the breeding efficiency is improved. Therefore, new hybridized germplasm with excellent characteristics of high fertilization rate, high hatching rate, high survival rate, high growth speed and the like is cultivated.

The invention discloses a bacillus coagulans feed additive and a preparation method thereof, and relates to the technical field of feed additives, the bacillus coagulans feed additive comprises bacillus coagulans spores, the content of the spores is 1.0 * 10 < 8 >-1.0 * 10 < 11 > CFU / g; the protective agent system comprises the following components: maltodextrin or lactose, vegetable protein, polyhydric alcohol and an antioxidant; the invention discloses a microcapsule coating structure. A microcapsule is a two-layer composite coating of an inner layer alginate matrix and an outer layer polycation or polymer coating. According to the invention, bacillus coagulans spores which can still keep high survival rate under short-time high-temperature and low-pH conditions are obtained through screening and fermentation strategies of strains with high sporulation and heat and acid tolerance, the problem that common strains are easy to inactivate in granulation, extrusion and gastric acid environments is solved, the problem of product failure caused by insufficient tolerance of the strains is reduced, and the product quality is improved. And the original biological activity basis is improved, so that the protection effect of the subsequent formula and the coating can be played at a higher starting point, and the zoology effect of the final preparation is improved.

The invention provides an in-situ ecological treatment method for recycling prawn culture tail water and application of the in-situ ecological treatment method, and belongs to the technical field of aquaculture wastewater treatment. After the miscellaneous shrimps in the culture water body are cleaned, quick lime is splashed to be matched with an iron chain to drag the bottom, and then a composite treating agent is put; inoculating rotifer, and then supplementing mineral substances; performing water quality verification; the composite treatment agent comprises EM bacteria, a seawater chlorella stock solution and a carbon source. According to the method, in-situ ecological treatment of prawn culture tail water and a zero-drainage culture mode are achieved, the treatment period is short, the culture survival rate is high, new treatment facilities are not needed, and beneficial bacterial algal phases and nutrient elements can be reserved.

Embodiments are directed to an advanced, modular, and scalable system integrating a Dissolved Gas Infusion (DGI) system and a Programmable Logic Controller (PLC) to automate and optimize processes such as water filtration, oxygenation, and waste management. The DGI system infuses high-purity oxygen directly into the water, maintaining optimal dissolved oxygen (DO) levels from the post-larvae stage to harvest, and significantly reducing oxygen wastage compared to traditional bubble aeration processes. The system includes modular raceways with load-bearing support beams, twist locks for secure stacking, and observation ports for easy access. This innovative approach ensures efficient, cost-effective, and reliable shrimp production, leading to higher survival rates, faster growth, and improved overall health of the shrimp.

The invention belongs to the field of functional composite microbial preparations, and particularly discloses a microbial agent and GABA compounded composition and application thereof in promoting growth and development of children. The composition is prepared from a freeze-dried bacterial agent and a GABA (gamma-aminobutyric acid) compound, wherein the freeze-dried bacterial agent comprises lactobacillus johnsonii YH1136 and lactobacillus helveticus Helveticus; the GABA compound is prepared by loading GABA with the purity of greater than or equal to 98% on a nano cellulose carrier, and the loading rate is 20-35%. The composition realizes high survival rate of the microbial inoculum and targeted slow release of GABA through a freeze-drying process and enteric coating. Experiments show that the composition can significantly improve the serum GH / IGF-1 level, the bone mineral density and the body growth rate, and has a synergistic effect when combined with the vitamin D3. According to the invention, the problems of single strain function, low GABA utilization rate and gastric acid damage in the prior art are solved, and an efficient and safe solution is provided for children growth and development intervention.

The invention discloses a Chinese wolfberry seedling raising method for improving paddy field soil, which comprises the following specific steps: collecting cutting slips: collecting branches with the diameter of more than 0.3 cm, cutting the branches into cutting slips with the length of 12-15 cm, and reserving 2-3 tender shoots on each cutting slip without damage; rooting liquid treatment: preparing a rooting liquid, and soaking the positions 3-5 cm above the bottom ends of the cutting slips in the rooting liquid for 12-24 hours; the rooting liquid is mainly prepared from rice vinegar and brown sugar; temperature-controlled root promoting: controlling the environment temperature to be 15-25 DEG C, and vertically inserting the cutting slips treated by the rooting liquid into the seedling raising container; the seedling raising container is filled with improved paddy field soil in advance; when 1-2 tender shoots newly grow on the cutting slips and 7-8 tender leaves grow on the cutting slips, shoot tips are removed, so that the tender shoots on the cutting slips are converted into semi-lignified from fleshy, apical dominance is inhibited, rooting is promoted, and seedling raising is continued; when new buds grow out again, seedling raising is completed, and the seedlings are transplanted to a field for planting. The lycium barbarum seedling raising method has the advantages of being low in cost, environmentally friendly, high in survival rate, suitable for improved paddy field soil and the like.

The invention discloses a passion fruit cutting and atomizing rapid seedling raising method. The method comprises the following steps: (1) preparing a seedling raising substrate; (2) selecting a cutting material; (3) treating before cutting; the passion fruit cuttage atomizing rapid breeding method is easy and convenient to operate, high in seedling emergence survival rate, high in survival rate after transplanting and worthy of application and popularization.

The invention relates to an artificial cross breeding method for male epinephelus fuscoguttatus and female humpback perch, which belongs to the technical field of distant cross breeding and comprises the steps of parent selection of the epinephelus fuscoguttatus and the humpback perch, parent ripening cultivation, gamete collection, artificial insemination and incubation and fry cultivation. By means of reproductive regulation and distant hybridization technologies, two species of two genera of grouper family in different geographical distribution and different reproduction time are hybridized, namely cross-generic hybridization of grouper family breaks through the generic reproductive isolation, and therefore good varieties which grow fast and are high in survival rate are cultivated.

The invention discloses a mucilaginosa N15 and a method for promoting the germination of dendrobium huoshanense seeds. The mucilaginosa N15 is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on October 27, 2025, and the preservation number of the strain is CGMCC No: 42290. The invention further discloses a method for promoting the germination of dendrobium huoshanense seeds by using the mucilaginosa N15 and the method for promoting the germination of dendrobium huoshanense seeds by using the mucilaginosa N15. The strain can be prepared into a high-activity microbial agent after activation and amplification culture, and the high-activity microbial agent is mixed with dendrobium huoshanense seeds and then sown in a greenhouse, so that the seed germination efficiency can be remarkably improved. By applying the method, the germination rate of the dendrobium huoshanense seeds can be increased to 60% or above, the mycorrhiza infection rate reaches 80% or above, the comprehensive cost is reduced by 30%, the seedling transplanting survival rate reaches 90% or above, and the seedling raising period is shortened to 12-15 months. The method is easy and convenient to operate, low in cost, high in transplanting survival rate and suitable for large-scale seedling raising and resource protection of dendrobium huoshanense, and has important economic and ecological values.

The invention provides a high-survival-rate forestry seedling raising method, and relates to the technical field of seedling raising. The method specifically comprises the following steps: S1, selecting seeds: selecting full and undamaged seeds with excellent genetic quality, soaking the seeds in a 0.3% potassium permanganate solution for 20 minutes, washing the seeds with clear water for three times, refrigerating the seeds for 15 days at 4 DEG C by adopting a variable-temperature stratification method, and sowing the seeds in a warm and humid environment of 15-20 DEG C for 5 days when the whitening rate reaches 80%; the method comprises the following steps: for hard-shell seeds, etching a micro-channel (the aperture is less than or equal to 10 microns) in a seed shell by adopting low-power pulse laser; coating the outer layers of the treated seeds with natural probiotics, burying the treated seeds into a simulated forest ant nest surface layer structure, and recovering the seeds with white buds after three weeks at constant temperature and humidity (22 + / -2 DEG C and humidity of 80%); s2, seedling culture on a seedling culture foundation bed. According to the method, in the whole process from the seeds to the nursery stocks, natural simulation, micro-ecology and reasonable regulation measures are adopted, the stress resistance and the survival rate of the nursery stocks are effectively improved, and the risk of nursery stock survival and subsequent management is reduced.

The invention belongs to the field of long-stalked gynostemma pentaphyllum cuttings and provides a long-stalked gynostemma pentaphyllum cutting method and a cultivation device thereof. The cutting process comprises: selecting healthy and sturdy middle and upper stem segments of long-stalked gynostemma pentaphyllum in a planting field, placing the segments in a tray or a basket, and storing them in a cool and shady environment; retaining two nodes on each cutting, cutting ends 1.5-2 cm apart from the upper and lower nodes, removing leaves and tendrils on the lower nodes, retaining a compound leaf on the upper portion, and preventing leaves and stems from being broken during the operation; then fixing the cut cuttings in a container with a water matrix, arranging them in order, separating rows with partitions, and ensuring that the water matrix is ​​10 cm deep, keeping the lower nodes 2-3 cm in the water matrix, transplanting 1000 cuttings per square meter, and using a sunshade net with a 50% sunshade rate for sunshade. This method uses water as the matrix, has a high survival rate of cuttings, does not require other matrices such as peat, is easy to obtain, has little pollution, and has a propagation efficiency that is more than 5 times that of other methods. It significantly reduces labor costs and is conducive to the rapid propagation of long-stemmed Gynostemma pentaphyllum.

The invention relates to the technical field of sweet potato stock seed propagation, in particular to a sweet potato virus-free seed potato one-year efficient rapid propagation method which comprises the following steps: (1) culturing virus-free test-tube plantlets; (2) producing greenhouse hydroponic seedlings; and (3) breeding the virus-free potato seeds in the net room. Through combination of a specific rooting nutrient solution and a seedling strengthening nutrient solution and the method, virus-free potato seed supply can be completed within one year, seed property degradation can be effectively overcome, stress resistance is improved, excellent characteristics of varieties are reproduced, and the method has the advantages of being short in production period, low in production cost, large in propagation coefficient, flourishing in branches and leaves, developed in root system, high in survival rate, high in economic benefit and the like. The finally prepared detoxified sweet potato seeds are uniform in size, high in germination rate, consistent in growth vigor, stable in character and suitable for large-scale production.

The invention discloses a preparation method and application of athlete-derived bifidobacterium probiotics for intestinal barrier repair, and relates to the technical field of biochemistry and microorganisms, and the preparation method comprises the steps of special bacteria source sampling, directional separation and purification, molecular biological identification, antibiotic sensitivity evaluation and injury repair verification. According to the preparation method and application of the sportsman-derived bifidobacterium probiotics for repairing the intestinal barrier, the sportsman intestinal flora is used as an exclusive bacterial source, and the unique metabolic elite attribute and stress adaptability of the sportsman intestinal flora are utilized to directionally screen and obtain a bifidobacterium strain adaptive to an extreme physiological stress environment; the bottleneck that conventional probiotics are only suitable for a common physiological environment and the survival rate and activity in a stress state are greatly reduced is broken through, high survival rate and metabolic activity can be kept in extreme environments such as exercise heat stress and the like, and accurate repair of intestinal barriers is realized through multiple mechanisms; meanwhile, an exclusive screening, identification and safety evaluation system is established, so that the safety and functional specificity of the strain are ensured.

The application discloses a functional probiotic microencapsulated intestinal shield system and a preparation method and application thereof. A core layer comprises four probiotics, i.e., lactobacillus acidophilus La-14, animal bifidobacterium Bb-12, lactobacillus rhamnosus GG and lactobacillus reuteri DSM 17938, and the weight ratio is 3:4:2:1, and the total viable bacterial count is greater than or equal to 10^10 CFU / g. An inner layer is a targeted release control layer, which is composed of ethyl cellulose, polylactic acid, chitosan and lactose, can swell in a pH 5.5 to 6.5 environment and can dissolve in a pH 6.8 to 7.2 environment. A middle layer is a buffer protection layer, which is composed of calcium carbonate, disodium hydrogen phosphate and sodium alginate, and can maintain the internal pH of the microcapsule at 4.5 to 5.5 in a gastric acid environment. An outer layer is an intelligent response protection layer, which is composed of konjac glucomannan, xanthan gum and gum arabic, and can form a protective film in a gastric acid environment with a pH less than 3.0 within 5 minutes. Through the synergistic protection of the four-layer microcapsule structure, the probiotics can achieve a high survival rate in the gastric acid environment of cats, and can be accurately released at specific positions in the intestinal tract, which is helpful to improve the intestinal health of cats.

The invention relates to the technical field of plant cultivation, in particular to a high-survival-rate seedling transplanting method. The transplanting method comprises the following steps: classifying and screening nursery stocks to obtain screened nursery stocks; lifting the screened seedlings according to a preset soil ball diameter to obtain a seedling transport body; loading the nursery stock transportation body into a container containing a pretreated nursery substrate (perlite, dried chicken manure, peat soil, straw and decomposed sweet potato seedlings) to obtain a nursery stock transplanting body; transferring the nursery stock transplanting body into the planting ditch for bed arrangement operation to obtain a nursery stock transplanting bed body; performing early-stage management on the nursery stock transplanting bed body to obtain a nursery stock bed; in the early-stage management, a compound organic fertilizer (rape seed cake, sheep manure, humic acid, wheat straw, bentonite, boric acid, magnesium sulfate, ammonium sulfate, calcium superphosphate, potassium sulfate and water) is used for additional fertilization; transplanting and planting the nursery stock bed to obtain transplanted nursery stocks. According to the method, by constructing a technical closed loop of root system microenvironment restoration, gradient adaptation and precise nutrition, the transplanting survival rate of the seedlings can be effectively increased.

The invention discloses a nutrition bowl for desert tree planting and a preparation method thereof, and belongs to the technical field of nutrition bowls for trees. Cow dung, straw and other raw materials are mixed with loess to prepare a concave bowl, and then polylactic acid films with different degradation rates are prepared to coat the concave bowl, so that the concave bowl becomes a nutrition bowl for water storage and fertilizer conservation; when the fertilizer is used in cooperation in the desert tree planting process, water loss can be effectively inhibited, the absorption and utilization rate of saplings to water and nutrients is improved, the transplanting survival rate of the saplings is increased, the growth condition of the saplings is improved, and therefore the desertification control effect is improved; the tree planting cost is greatly reduced, and the method has a good application prospect.

The application belongs to the field of food biotechnology, and particularly relates to a high-folate Lactobacillus plantarum ZFM4 microcapsule and a preparation method thereof. The method comprises the following steps: centrifuging and resuspending a fermentation liquor of high-folate Lactobacillus plantarum ZFM4 to obtain concentrated bacterial liquid; uniformly mixing the concentrated bacterial liquid with a composite wall material and then adding the mixture into a calcium chloride solution to obtain wet Lactobacillus plantarum single-layer microcapsules; the composite wall material is composed of sodium alginate, cassava starch and water; and wet double-layer Lactobacillus plantarum microcapsules are obtained by adding the wet Lactobacillus plantarum single-layer microcapsules into a chitosan solution. The high-folate Lactobacillus plantarum microcapsule prepared by the method has good acid resistance, high survival rate of lactic acid bacteria and high embedding rate, and has a high application prospect.

The invention discloses a field capturing, short-term storing and feeding device for blood sucking midges and a using method thereof. The device is composed of a living body maintaining pipe, a sealing cover and a base. The living body maintaining tube is placed on the base; and a sealing cover is arranged at the top of the living body maintaining tube. The use method comprises the following steps: capturing living blood sucking midges in the field by utilizing the capturing state of the device; the device is converted into a feeding state, that is, a rubber plug fixed with an insect inlet unit and an air suction unit is removed, and a threaded semi-sealing cover with a feeding filter screen is installed; nutrient solution is provided for the gnat in the living body maintaining pipe through the feeding filter screen. The field catching, short-term storage and feeding integrated device overcomes the defects that an existing blood sucking midge collecting device cannot give consideration to the high catching rate, the high survival rate and the short-term storage and feeding function, and is simple in structure, convenient and fast to operate and capable of remarkably improving the survival rate of gnat from the catching stage to the laboratory stage.

The present invention discloses a high survival rate method for grafting Pinus sylvestris var. mongolica onto Pinus tibetana in high-altitude cold regions, which improves the adaptability and survival rate of grafting through multi-dimensional innovation. A double interface with an ascending slope on the sunny side of the Pinus sylvestris var. mongolica stock and a descending slope on the shady side is provided to match the double wedge-shaped part of the scion bud of Pinus tibetana to improve the contact surface between the stock and the scion; a photothermal responsive shape memory polymer strap is used for fixation to inhibit pathogens and promote callus differentiation; a double-layer protective structure is constructed, with a built-in moisture control group in the inner moisturizing and breathable membrane to maintain the interface humidity ≥ 60%, and an outer anti-UV windproof net to intelligently adjust to cope with wind, sand and strong light. Further, callus differentiation is precisely regulated by pre-embedded pH-responsive cellulose microspheres, or an intelligent temperature control membrane and an aerogel layer are added to stabilize the temperature difference, and the scion bud is combined with low-temperature acclimation and abscisic acid treatment to enhance frost resistance. This method significantly improves the grafting survival rate and is suitable for the efficient introduction of Pinus tibetana in high-altitude cold regions, with strong process synergy and high environmental adaptability.

[Problem] To improve production efficiency in the breeding and farming of crustaceans.　[Solution] Provided are a method for improving the survival rate of crustaceans, a method for producing crustaceans, etc., in which a soybean-isoflavone-containing crustacean feed is fed to crustaceans. In the breeding and farming of crustaceans, a high survival rate during the breeding period can be maintained by feeding a soybean-isoflavone-containing crustacean feed to the crustaceans. Specifically, the number of deaths of crustaceans introduced into breeding and farming can be reduced, and, for example, the number of crustaceans that grow to a level suitable for shipping can be increased; therefore, the production efficiency in the breeding and farming of crustaceans can be improved.

The present invention provides a strain of Escherichia coli resistant to nicotinamide stress and its application, which belongs to the field of microbial engineering technology. The strain is Escherichia coli AE74, which has been deposited in the China Center for Type Culture Collection on December 23, 2022, with a deposit number of CCTCC NO: M 20222059. The strain resistant to nicotinamide (NAM) stress has both excellent anti-nicotinamide performance and high nicotinamide mononucleotide (NMN) yield. On the one hand, under non-nicotinamide stress conditions, strain AE74 has a higher NMN yield than the starting strain. On the other hand, strain AE74 overcomes the problem of low NAM tolerance of the starting strain, and can maintain a high survival rate and NMN yield at high NAM concentrations, providing a new strain with high NMN yield suitable for industrial production.

The application discloses a breeding method for improving the quality of Sebastes schlegelii fry, and comprises the following steps: collecting parent fish in a Sebastes schlegelii original seed field, artificially cultivating the parent fish in a net cage at sea, and performing nutrition rejuvenation on the parent fish; obtaining offspring fry by using an artificial fry squeezing method; controlling reasonable culture density according to different fry specifications; screening and using natural plant active ingredients and common micro-ecological preparations which are easy to absorb; avoiding disinfection and drug use; and controlling water flow rate in different growth stages. The method integrates nutrition rejuvenation, artificial fry production, pollution-free water quality regulation and fry behavior state regulation, solves problems such as poor parent germplasm, dead fry or insufficient activity of fry, slow growth and poor stress resistance in the process of fry breeding, has strong controllability in the operation period, has high survival rate of fry, has remarkable growth promotion and immunity effect, has good prevention and treatment effect on bacterial diseases, realizes pollution-free fry breeding, can reduce disease occurrence and mortality, reduces the feed conversion ratio, and effectively improves the economic benefit of breeding.

The invention discloses a micro-cutting seedling raising method for Momordica grosvenori, which belongs to the technical field of cutting seedling raising. The method comprises the following steps: regulating the length of stem nodes of a mother plant by using a stem control and root inducing agent, performing dark treatment to obtain pretreated vines; taking the pretreated vines as cutting materials; dipping the base of the cutting materials in yellow mud slurry, which is an antiseptic and root-inducing agent; cutting or burying the cuttings in a matrix containing vermiculite, grass charcoal soil, and pine needle segments; culturing the cuttings in an environment with a light transmittance of 10%-15%, a temperature of 25-30°C, and an air relative humidity of 80-90%, and spraying the cuttings with an aqueous solution of the stem control and root inducing agent once every 5-7 days after new buds unfold their leaves. The present invention develops a stem-controlling and root-inducing agent and an antiseptic and root-growing agent based on the growth characteristics of the winged momordica grosvenori. The stem-controlling and root-inducing agent is used to regulate the growth of stem nodes, and the light and dark treatment is used to induce the rooting of the stem nodes. The invention uses less material and has a high utilization rate, is simple to operate, and has a high survival rate. The plant can be bred from March to October, breaking the limitation that seedlings can only be raised by pressing potatoes in autumn, and solving the problem of low survival rate of the winged momordica grosvenori cuttings.

The utility model relates to the technical field of vegetable transplanting equipment, in particular to vegetable transplanting equipment with high survival rate. Vegetable transplanting equipment high in survival rate comprises a mounting frame and the like, a valve pipe is connected to the middle of the bottom of the mounting frame, four universal wheels are fixedly connected to the bottom of the mounting frame at intervals, a handle is fixedly connected to the right side of the mounting frame, and the vegetable transplanting equipment further comprises a placing assembly and a lifting assembly. The water leakage holes are formed in the placement plate, water which cannot be absorbed by soil at the roots of vegetable seedlings can flow into the mounting frame from the water leakage holes, the situation that the soil at the roots of the vegetable seedlings is excessively soaked by the water is effectively avoided, a good foundation is laid for subsequent transplanting work, and after the placement plate is lifted to a certain height, the water leakage holes are formed in the placement plate. According to the vegetable seedling lifting device, a worker can lift soil at the roots of vegetable seedlings, so that extrusion and damage to the soil are reduced, the integrity of root systems of the seedlings is protected, and the survival rate of transplanted vegetables is greatly increased.

The invention belongs to the technical field of Chinese cabbage seedling raising or Chinese cabbage cultivation, and particularly relates to a method for achieving the high seedling emergence survival rate of low-temperature vernalization Chinese cabbage seeds. According to the method, the seeds with overlong buds after low-temperature vernalization are subjected to three-dimensional vertical cultivation, the emergence rate and the survival rate of the Chinese cabbage seeds can be remarkably increased, the emergence survival rate is 90%-99%, and the emergence survival rate is increased by nearly 70% compared with the emergence survival rate of 29.37% in a conventional direct seeding mode.

The present invention belongs to the field of biotechnology, and discloses a Bacillus subtilis strain from Tibetan pigs, with the classification name of Bacillus subtilis Yirun2024, the Latin name of Bacillus subtilis Yirun2024, the preservation number of CCTCC NO: M 20241752, the preservation date of August 7, 2024, the preservation unit of China Center for Type Culture Collection, and the preservation address of Wuhan University, Wuhan, China. This Bacillus subtilis has high cellulase production. After 12 h of fermentation in a shake flask, the activities of endoglucanase, exoglucanase and β-glucosidase are respectively as high as 2902.72 U / mL, 11026.33 U / mL and 2983.04 U / mL, far exceeding those of similar engineering bacteria. At the same time, the present invention also provides the application of this Bacillus subtilis, which is of great significance in promoting the application field of fiber resources. At the same time, this strain can maintain a relatively high survival rate in a high-temperature environment.

The application belongs to the technical field of fish genetic breeding, and specifically discloses an artificial induction method of Megalobranchius ambius and Culter alburnus hybrid triploid and application. The artificial induction method comprises the following steps: S1. taking Megalobranchius ambius as a female parent and Culter alburnus as a male parent to perform artificial dry insemination; S2. performing hydrostatic pressure treatment on the fertilized eggs, and the hydrostatic pressure treatment conditions are as follows: the starting time of treatment after fertilization is 2-3 min, the pressure is 35-50 MPa, and the duration is 3 min; S3. hatching the fertilized eggs and performing transfer culture, and the water temperature in the whole process from fertilization, hydrostatic pressure treatment, hatching to fry cultivation is controlled to be 22-24 DEG C; and Megalobranchius ambius and Culter alburnus triploid is screened through ploidy analysis. The method can successfully induce Megalobranchius ambius and Culter alburnus hybrid sterile triploid with superior growth performance, and based on comprehensive index evaluation, the best hydrostatic pressure treatment condition is obtained, high triploid rate (>80%) and high survival rate (>98%) are simultaneously achieved, and commercialized breeding production can be realized.

The invention relates to a germplasm cryopreservation method suitable for bait microalgae, and belongs to the field of algae preservation, and the method comprises the following steps: carrying out low-temperature domestication on microalgae in a logarithmic phase, and centrifugally collecting algae mud; mixing the algae mud with a protective solution for pretreatment; after pretreatment, mixing with a cryoprotectant with specific composition, carrying out gradient cooling, and storing at-80 DEG C; during thawing, the cryopreservation tube is quickly thawed in a water bath, and the cryoprotectant is subjected to gradient elution through a sucrose solution. The method successfully realizes long-term stable preservation of various bait microalgae at-80 DEG C by optimizing a cryoprotectant combination and optimizing key parameters such as a protective solution, balance time and thawing temperature, has high survival rate and stable physiological traits after resuscitation, and is low in cost, convenient to operate and suitable for industrial production. And a new technical scheme is provided for protection and utilization of microalgae germplasm resources.

The present application belongs to the technical field of plant cultivation, and particularly relates to a high-temperature-resistant planting method for Anoectochilus roxburghii, which comprises the following steps in sequence: semi-closed planting device, sterilization, substrate preparation, addition of specific nutrient solution, tissue culture seedling planting, and greenhouse facility setting. The method can enable the Anoectochilus roxburghii to safely endure summer at an extreme high temperature of 38 DEG C outside and 43 DEG C in the greenhouse, and an average monthly high temperature of 31 DEG C outside for 4 months, and grow well. The planted Anoectochilus roxburghii is not harmed by diseases and pests, and the Anoectochilus roxburghii planting process is simple to manage, has the advantages of simple operation, low management cost, high survival rate, and the like, and solves the problem of safe summer endurance of the precious medicinal plant Anoectochilus roxburghii in low-altitude and high-temperature areas, thereby providing technical support for expansion of the Anoectochilus roxburghii planting industry.

The invention relates to the field of rice gene engineering, and discloses an application of OsSGR1 protein and a coding gene thereof in regulation and control of rice salt tolerance. The gene of the OsSGR1 protein is edited on the basis of a CRISPR / Cas9 technology, an agrobacterium-mediated method is utilized to introduce a japonica rice variety Zhonghua 11, and a knockout mutant is obtained through screening; tests prove that the survival rate of the rice mutant with the OsSGR1 gene knocked out is remarkably higher than that of a wild type Zhonghua 11 plant after the rice mutant is subjected to stress treatment for 11 days by 150 mM NaCl and 200 mM NaCl salt and then is rehydrated for 7 days after the rice mutant is subjected to two-leaf and one-core stage. And a simple and effective technical means is provided for rapidly creating a new salt-tolerant rice strain. Therefore, the OsSGR1 protein and the coding gene thereof disclosed by the invention can regulate and control the salt tolerance of the rice, and are of great significance to the cultivation of salt-tolerant transgenic rice.