379 results about "High survival rate" patented technology

The invention relates to an artificial hybridization fry breeding method for epinephelus fuscoguttatus and pink pink bass, which belongs to the technical field of distant hybridization breeding and comprises the steps of parent selection of epinephelus fuscoguttatus and pink pink bass, parent breeding, parent intensive breeding, gamete collection, artificial insemination, hatching of fertilized eggs and fry breeding. By means of reproductive regulation and distant hybridization technologies, two species belonging to two genera under the grouper family with different geographical distribution and different breeding time are hybridized, namely cross-generic hybridization under the grouper family is achieved, intergeneric reproductive isolation is broken through, and the breeding efficiency is improved. Therefore, new hybridized germplasm with excellent characteristics of high fertilization rate, high hatching rate, high survival rate, high growth speed and the like is cultivated.

The invention belongs to the technical field of biology, and discloses a Tibetan pig source bacillus subtilis, the classification name is bacillus subtilis Yirun2024, the Latin name is bacillus subtilis Yirun2024, the preservation number is CCTCC NO: M 20241752, the preservation date is August 7, 2024, the preservation unit is China Center for Type Culture Collection, and the preservation address is Wuhan University, Wuhan, China. When the bacillus subtilis is fermented for 12 hours in a shake flask, the endo-cellulase activity, the exo-cellulase activity and the beta-glucosidase activity of the bacillus subtilis are respectively up to 2902.72 U / mL, 11026.33 U / mL and 2983.04 U / mL, which are far better than those of similar engineering bacteria. Meanwhile, the invention further provides application of the bacillus subtilis, the bacillus subtilis has important significance in the field of promotion of application of fiber resources, and meanwhile, the bacillus subtilis can keep a high survival rate in a high-temperature environment.

The invention relates to the technical field of agricultural microorganisms, in particular to a microbial agent containing paenibacillus love KF-B6, a compound microbial fertilizer and application of the microbial agent and the compound microbial fertilizer. The KF-B6 Paenibacillus lovuenyuan as well as the microbial agent and the compound microbial fertilizer thereof provided by the invention can promote the formation of soil aggregates, improve soil organic matters, improve soil enzyme activity, effectively improve soil and increase the proportion of soil bacteria and fungi. Therefore, the method can be applied to prevention and treatment of apple continuous cropping obstacles, and the survival rate of field planting of apple seedling stocks and field planting of young trees in a rebuilt orchard is guaranteed. By applying the KF-B6 compound microbial fertilizer and the microbial agent, the survival rate of the M9T337 rootstock seedlings planted in the continuous cropping soil can be increased, the highest survival rate reaches 96.67%, and the seedling roots are high in activity and good in growth vigor. The survival rate of the Daphne red apple seedlings planted in the rebuilding of the continuous cropping obstacle old orchard is as high as 94.00%, and the effect of effectively preventing and controlling the apple continuous cropping obstacle is achieved.

The invention discloses a high-survival-rate method for grafting pinus sylvestris on pinus sylvestris in an alpine region. The grafting adaptability and the survival rate are improved through multi-dimensional innovation. A rising slope is arranged on the sunny side of a pinus sylvestris rootstock, double connectors of a falling slope are arranged on the shady side of the pinus sylvestris rootstock and matched with double wedge-shaped parts of Tibetan white bark pine scion buds, and the rootstock-scion contact face is increased; a photo-thermal response shape memory polymer bandage is adopted for fixation, germs are inhibited, and callus differentiation is promoted; a double-layer protection structure is constructed, a humidity control group is arranged in an inner-layer moisturizing breathable film to maintain the humidity of a connector to be larger than or equal to 60%, and an outer-layer anti-UV windproof net is intelligently adjusted to cope with sand wind and strong light. Furthermore, the callus differentiation is accurately regulated and controlled by pre-embedding pH responsive cellulose microspheres, or an intelligent temperature control film and an aerogel layer are additionally arranged to stabilize the temperature difference, and the freezing resistance is enhanced in combination with scion bud low-temperature domestication and abscisic acid treatment. The method remarkably improves the grafting survival rate, is suitable for efficient introduction of the Tibetan white bark pine in the alpine region, and is high in process synergy and high in environmental adaptability.

The invention discloses a bacillus coagulans feed additive and a preparation method thereof, and relates to the technical field of feed additives, the bacillus coagulans feed additive comprises bacillus coagulans spores, the content of the spores is 1.0 * 10 < 8 >-1.0 * 10 < 11 > CFU / g; the protective agent system comprises the following components: maltodextrin or lactose, vegetable protein, polyhydric alcohol and an antioxidant; the invention discloses a microcapsule coating structure. A microcapsule is a two-layer composite coating of an inner layer alginate matrix and an outer layer polycation or polymer coating. According to the invention, bacillus coagulans spores which can still keep high survival rate under short-time high-temperature and low-pH conditions are obtained through screening and fermentation strategies of strains with high sporulation and heat and acid tolerance, the problem that common strains are easy to inactivate in granulation, extrusion and gastric acid environments is solved, the problem of product failure caused by insufficient tolerance of the strains is reduced, and the product quality is improved. And the original biological activity basis is improved, so that the protection effect of the subsequent formula and the coating can be played at a higher starting point, and the zoology effect of the final preparation is improved.

The invention provides an in-situ ecological treatment method for recycling prawn culture tail water and application of the in-situ ecological treatment method, and belongs to the technical field of aquaculture wastewater treatment. After the miscellaneous shrimps in the culture water body are cleaned, quick lime is splashed to be matched with an iron chain to drag the bottom, and then a composite treating agent is put; inoculating rotifer, and then supplementing mineral substances; performing water quality verification; the composite treatment agent comprises EM bacteria, a seawater chlorella stock solution and a carbon source. According to the method, in-situ ecological treatment of prawn culture tail water and a zero-drainage culture mode are achieved, the treatment period is short, the culture survival rate is high, new treatment facilities are not needed, and beneficial bacterial algal phases and nutrient elements can be reserved.

Embodiments are directed to an advanced, modular, and scalable system integrating a Dissolved Gas Infusion (DGI) system and a Programmable Logic Controller (PLC) to automate and optimize processes such as water filtration, oxygenation, and waste management. The DGI system infuses high-purity oxygen directly into the water, maintaining optimal dissolved oxygen (DO) levels from the post-larvae stage to harvest, and significantly reducing oxygen wastage compared to traditional bubble aeration processes. The system includes modular raceways with load-bearing support beams, twist locks for secure stacking, and observation ports for easy access. This innovative approach ensures efficient, cost-effective, and reliable shrimp production, leading to higher survival rates, faster growth, and improved overall health of the shrimp.

The invention relates to the technical field of microorganisms, in particular to lactobacillus rhamnosus HZBX-05 and application thereof.The lactobacillus rhamnosus HZBX-05 is sent to the China General Microbiological Culture Collection Center on July 27th, 2023 to be preserved in the China General Microbiological Culture Collection Center, the preservation number of the lactobacillus rhamnosus HZBX-05 is CGMCC NO.987, and the preservation number of the lactobacillus rhamnosus HZBX-05 is CGMCC NO.989. The lactobacillus rhamnosus HZBX-05 is CGMCC NO.989. The lactobacillus rhamnosus HZBX-05 is CGMCC NO.989. The preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.28035, and the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing; the separated Lactobacillus rhamnosus HZBX-05 is continuously treated for 7 hours in a simulated gastric acid cholate environment, and the result shows that the HZBX-05 still has high survival rate, has the capability of being highly adsorbed to intestinal cells Caco-2 cells, has the capability of inhibiting various pathogenic bacteria, has immunoregulation and anti-inflammation effects, has the effect of resisting helicobacter pylori, and can be used for preparing an anti-inflammatory drug. The symptoms of female vaginitis can be improved.

The invention discloses an ecological restoration method for a subalpine meadow side slope after engineering disturbance, and the method comprises the steps: transplanting local native turf to a specific position of the side slope in a chessboard type layout mode, and stripping and filling the soil of the native turf into an unoccupied area except the transplanted turf, so that a local ecological system can be quickly restored, and the ecological restoration of the subalpine meadow side slope is facilitated. Meanwhile, the balance of a local ecological system is maintained, and the service function of the local ecological system is restored. According to the method, the local native turf is transplanted in a chessboard type layout mode, manpower and material resources are effectively saved, meanwhile, half of the native turf is transplanted to drive an original seed bank in soil to grow, vegetation coverage of the whole restoration area is promoted to be rapidly achieved, native vegetation is used for restoration, harm caused by using alien species is avoided, and the method is suitable for large-scale popularization and application. Meanwhile, the method has the advantages of low cost, high recovery speed, high survival rate and the like, and the purposes of recovering an ecological system by using local species and seed banks and recovering nature in a natural manner are achieved.

The invention relates to application of polypeptide in preparation of a feed additive for improving disease resistance of prawns, and belongs to the technical field of aquaculture. The polypeptide is anglerfish bone polypeptide, and the molecular weight of the anglerfish bone polypeptide is 1000-5000 Da. The anglerfish bone polypeptide prepared by fermentation pretreatment and enzymolysis processes can effectively improve the growth performance, disease resistance and immune function of litopenaeus vannamei. The prawns fed with the feed additive show significant final weight gain and relatively high survival rate, and especially show strong resistance when coping with infection of common pathogenic bacteria such as vibrio parahaemolyticus and the like. Therefore, the survival rate and the production efficiency of the prawns in the culture process are improved, the use of antibiotics can be reduced, the drug residues and the drug resistance of pathogenic bacteria are reduced, and the development of the green and sustainable prawn culture industry is promoted.

The invention provides a seedling planting device for rice planting, and relates to the field of rice transplanting devices. The seedling planting device for rice planting comprises a seedling storage assembly, a seedling transplanting assembly, a power assembly and a linkage assembly, and a vibration assembly is mounted on one side of the bottom surface of the seedling storage assembly; the seedling storage assembly comprises a seedling box, one end of the seedling box is open, the other end of the seedling box is of an arc-shaped contraction structure, and a vertical groove is formed in the arc-shaped contraction structure of the seedling box and communicates the inside and the outside of the seedling box. According to the seedling planting device for rice planting, by arranging the seedling transplanting assembly and the linkage assembly, the roots of seedlings cannot be damaged when the seedlings are moved out of the bottoms of the vertical grooves, so that the high survival rate of seedling transplanting can be achieved, the seedlings can be immediately separated from the pointed cones when being brought out, and the seedlings cannot be brought out of soil again; when the depth is in place, the pointed cones are in a longitudinal state, so that the seedlings are prevented from lodging when being transplanted.

The invention discloses a high-survival-rate grafting method for grafting pinus sylvestris to pinus koraiensis in arid regions, which comprises the following steps: selecting pinus sylvestris stock with the growth period of 4-6 years, flatly cutting below a terminal bud branch point to form a cross section, downwards and obliquely cutting twice from the cross section to form a triangular pyramid-shaped chimeric structure, cutting a semi-lignified pinus koraiensis scion with a terminal bud, and grafting the semi-lignified pinus koraiensis scion with the terminal bud to obtain the pinus sylvestris scion. After main buds are reserved and lateral buds are cut off, two oblique triangular xylem sections are obliquely cut out from the lower portion of spores through two cutters, and a double-slope wedge-shaped joint body is formed; when the rootstock and the scion are cut, the cutting blade is immersed in an aqueous solution containing a plant growth regulator and a bactericide; the double-slope wedge-shaped joint body is inserted into the triangular pyramid-shaped embedding structure, so that the scion medullary layer and the stock medullary layer are overlapped; wrapping the interface with a composite hydrogel film containing a temperature-sensitive polymer and an antibacterial component; shading treatment is carried out after grafting is completed. The method can significantly improve the survival rate of grafted seedlings.

The invention belongs to the field of functional composite microbial preparations, and particularly discloses a microbial agent and GABA compounded composition and application thereof in promoting growth and development of children. The composition is prepared from a freeze-dried bacterial agent and a GABA (gamma-aminobutyric acid) compound, wherein the freeze-dried bacterial agent comprises lactobacillus johnsonii YH1136 and lactobacillus helveticus Helveticus; the GABA compound is prepared by loading GABA with the purity of greater than or equal to 98% on a nano cellulose carrier, and the loading rate is 20-35%. The composition realizes high survival rate of the microbial inoculum and targeted slow release of GABA through a freeze-drying process and enteric coating. Experiments show that the composition can significantly improve the serum GH / IGF-1 level, the bone mineral density and the body growth rate, and has a synergistic effect when combined with the vitamin D3. According to the invention, the problems of single strain function, low GABA utilization rate and gastric acid damage in the prior art are solved, and an efficient and safe solution is provided for children growth and development intervention.

The invention discloses a Chinese wolfberry seedling raising method for improving paddy field soil, which comprises the following specific steps: collecting cutting slips: collecting branches with the diameter of more than 0.3 cm, cutting the branches into cutting slips with the length of 12-15 cm, and reserving 2-3 tender shoots on each cutting slip without damage; rooting liquid treatment: preparing a rooting liquid, and soaking the positions 3-5 cm above the bottom ends of the cutting slips in the rooting liquid for 12-24 hours; the rooting liquid is mainly prepared from rice vinegar and brown sugar; temperature-controlled root promoting: controlling the environment temperature to be 15-25 DEG C, and vertically inserting the cutting slips treated by the rooting liquid into the seedling raising container; the seedling raising container is filled with improved paddy field soil in advance; when 1-2 tender shoots newly grow on the cutting slips and 7-8 tender leaves grow on the cutting slips, shoot tips are removed, so that the tender shoots on the cutting slips are converted into semi-lignified from fleshy, apical dominance is inhibited, rooting is promoted, and seedling raising is continued; when new buds grow out again, seedling raising is completed, and the seedlings are transplanted to a field for planting. The lycium barbarum seedling raising method has the advantages of being low in cost, environmentally friendly, high in survival rate, suitable for improved paddy field soil and the like.

The invention discloses a lactobacillus johnsonii and related products and applications thereof, and relates to the field of microorganisms. The Lactobacillus johnsonii GJ231 provided by the invention has a preservation number of CCTCC NO: M 2023981. Compared with other lactobacillus johnsonii, GJ231 is acid-resistant and bile-resistant, has a higher survival rate in the intestine, has a better antibacterial effect on a variety of pathogens, and can be used to prevent and treat related diseases caused by pathogens; secondly, GJ231 has a better high temperature tolerance and has a higher survival rate in the process of spraying and preparing pet food; furthermore, GJ231 also has a strong antioxidant capacity, can reduce the peroxidation reaction caused by oxidative stress, and can be used for anti-aging and anti-oxidation; in addition, GJ231 also has a bacteriocin gene cluster, which may be used to produce bacteriocin, and has the potential to replace antibiotics and food preservation.

The invention discloses a passion fruit cutting and atomizing rapid seedling raising method. The method comprises the following steps: (1) preparing a seedling raising substrate; (2) selecting a cutting material; (3) treating before cutting; the passion fruit cuttage atomizing rapid breeding method is easy and convenient to operate, high in seedling emergence survival rate, high in survival rate after transplanting and worthy of application and popularization.

The invention relates to an artificial cross breeding method for male epinephelus fuscoguttatus and female humpback perch, which belongs to the technical field of distant cross breeding and comprises the steps of parent selection of the epinephelus fuscoguttatus and the humpback perch, parent ripening cultivation, gamete collection, artificial insemination and incubation and fry cultivation. By means of reproductive regulation and distant hybridization technologies, two species of two genera of grouper family in different geographical distribution and different reproduction time are hybridized, namely cross-generic hybridization of grouper family breaks through the generic reproductive isolation, and therefore good varieties which grow fast and are high in survival rate are cultivated.

The invention discloses seedling and breeding equipment for mandarin fish culture, and relates to the technical field of aquaculture equipment. The invention provides a siniperca chuatsi breeding device with high survival rate, which comprises a box body and the like, the box body is fixedly connected with a temperature control plate, the box body is fixedly connected with paired air pumps, the box body is fixedly connected with paired spray heads, the air pumps are fixedly connected and communicated with the spray heads, the box body is fixedly connected with paired fixing frames which are longitudinally distributed, the fixing frames are rotatably connected with connecting shafts, and the connecting shafts are fixedly connected with the box body. The connecting shaft is fixedly connected with pairwise colored ribbons, the box body is fixedly connected with a partition plate, the partition plate is rotatably connected with pairwise hinged plates which are longitudinally distributed, and pairwise torsional springs are fixedly connected between the hinged plates and the partition plate. By means of the colored ribbons, the hinged plates and other parts, the activity degree of the mandarin fish fries can be improved, the fries of different specifications can be distinguished and separated by means of habits of the mandarin fish fries, the situations that the fries grab food and are disable in food due to the difference of the specifications of the fries are avoided, and the breeding survival rate of the seedling breeding equipment for mandarin fish breeding is increased.

The invention discloses a mucilaginosa N15 and a method for promoting the germination of dendrobium huoshanense seeds. The mucilaginosa N15 is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on October 27, 2025, and the preservation number of the strain is CGMCC No: 42290. The invention further discloses a method for promoting the germination of dendrobium huoshanense seeds by using the mucilaginosa N15 and the method for promoting the germination of dendrobium huoshanense seeds by using the mucilaginosa N15. The strain can be prepared into a high-activity microbial agent after activation and amplification culture, and the high-activity microbial agent is mixed with dendrobium huoshanense seeds and then sown in a greenhouse, so that the seed germination efficiency can be remarkably improved. By applying the method, the germination rate of the dendrobium huoshanense seeds can be increased to 60% or above, the mycorrhiza infection rate reaches 80% or above, the comprehensive cost is reduced by 30%, the seedling transplanting survival rate reaches 90% or above, and the seedling raising period is shortened to 12-15 months. The method is easy and convenient to operate, low in cost, high in transplanting survival rate and suitable for large-scale seedling raising and resource protection of dendrobium huoshanense, and has important economic and ecological values.

The invention provides a high-survival-rate selenium-rich apricot seedling transplanting and field planting method, and relates to the field of apricot seedling transplanting and field planting. The high-survival-rate selenium-rich apricot seedling transplanting and field planting method comprises the steps that S1, selenium-rich apricot seedlings which grow robustly and are free of diseases and insect pests are selected according to the selection standard that the seedling height ranges from 30 cm to 40 cm, and the stem thickness ranges from 0.5 cm to 0.8 cm; through selection of robust nursery stocks, root pruning and soaking treatment, the sensitivity of the nursery stocks to the transplanting environment is reduced, the adaptability is improved, through root soaking treatment and appropriate root pruning, the nursery stocks are not prone to mechanical damage in the transplanting process, through scientific fertilization and watering management, selenium-rich nutrition is provided, and the survival rate of the nursery stocks is increased. According to actual tests, the method can improve the survival rate of the selenium-rich apricot seedlings to 90% or above, and continuous inspection and management including fertilization, watering, pest and disease control and soil loosening and weeding after transplanting ensure continuous and healthy growth of the seedlings after field planting.

The invention provides a high-survival-rate forestry seedling raising method, and relates to the technical field of seedling raising. The method specifically comprises the following steps: S1, selecting seeds: selecting full and undamaged seeds with excellent genetic quality, soaking the seeds in a 0.3% potassium permanganate solution for 20 minutes, washing the seeds with clear water for three times, refrigerating the seeds for 15 days at 4 DEG C by adopting a variable-temperature stratification method, and sowing the seeds in a warm and humid environment of 15-20 DEG C for 5 days when the whitening rate reaches 80%; the method comprises the following steps: for hard-shell seeds, etching a micro-channel (the aperture is less than or equal to 10 microns) in a seed shell by adopting low-power pulse laser; coating the outer layers of the treated seeds with natural probiotics, burying the treated seeds into a simulated forest ant nest surface layer structure, and recovering the seeds with white buds after three weeks at constant temperature and humidity (22 + / -2 DEG C and humidity of 80%); s2, seedling culture on a seedling culture foundation bed. According to the method, in the whole process from the seeds to the nursery stocks, natural simulation, micro-ecology and reasonable regulation measures are adopted, the stress resistance and the survival rate of the nursery stocks are effectively improved, and the risk of nursery stock survival and subsequent management is reduced.

The invention belongs to the technical field of biological seedling culture, and particularly relates to a seedling culture method with high survival rate, which comprises the following steps: collecting agricultural wastes such as rice husk, wheat straw, corn straw and the like, performing crushing and fermentation primary treatment, performing disinfection treatment on the treated agricultural wastes, and killing pathogenic microorganisms and pests in the agricultural wastes; a proper amount of wood chips, coco coir, compost and other biomass materials are selected, the prepared agricultural waste and biomass materials are mixed according to a certain proportion, then beneficial microorganisms and natural organic additives are added, the mixed substrate is subjected to stacking fermentation, and finally the substrate is used for the seedling raising process of nursery stocks. The air permeability and the water-retaining property of the substrate can ensure healthy growth of seedling root systems, and stable nutrition supply is provided. The fertility, the air permeability and the biological activity of the substrate are optimized, the survival rate and the growth speed of seedlings are remarkably increased, and the seedling raising period is shortened.

The invention discloses a method for cultivating red nine-spined perch fry with a high survival rate, and relates to the technical field of marine fish breeding. The cultivation method comprises the following steps: (1) regulating the light source environment: using a blue light source with a wavelength range of 420-450nm to irradiate the red nine-spined perch fry for 60-75 days during the growth process; (2) transforming the bottom environment: laying a layer of artificial materials on the bottom of the water, introducing a water body with a salinity of 30-35‰ as a breeding water body, and using an oxygen pump for aeration throughout the day; (3) feeding A feed and B feed alternately: first feed A feed for 8-10 days, then feed A feed for 8-10 days, and feed alternately until the juvenile stage; A feed is prepared by adding oxalis to the basic feed, and B feed is prepared by adding loperamide to the basic feed. By means of the three parallel measures of regulating the light source + bottom transformation + alternating feeding, it is beneficial to improve the survival rate of red nine-spined perch fry, thereby improving the economic benefits.

The invention belongs to the field of long-stalked gynostemma pentaphyllum cuttings and provides a long-stalked gynostemma pentaphyllum cutting method and a cultivation device thereof. The cutting process comprises: selecting healthy and sturdy middle and upper stem segments of long-stalked gynostemma pentaphyllum in a planting field, placing the segments in a tray or a basket, and storing them in a cool and shady environment; retaining two nodes on each cutting, cutting ends 1.5-2 cm apart from the upper and lower nodes, removing leaves and tendrils on the lower nodes, retaining a compound leaf on the upper portion, and preventing leaves and stems from being broken during the operation; then fixing the cut cuttings in a container with a water matrix, arranging them in order, separating rows with partitions, and ensuring that the water matrix is ​​10 cm deep, keeping the lower nodes 2-3 cm in the water matrix, transplanting 1000 cuttings per square meter, and using a sunshade net with a 50% sunshade rate for sunshade. This method uses water as the matrix, has a high survival rate of cuttings, does not require other matrices such as peat, is easy to obtain, has little pollution, and has a propagation efficiency that is more than 5 times that of other methods. It significantly reduces labor costs and is conducive to the rapid propagation of long-stemmed Gynostemma pentaphyllum.

The invention relates to the technical field of sweet potato stock seed propagation, in particular to a sweet potato virus-free seed potato one-year efficient rapid propagation method which comprises the following steps: (1) culturing virus-free test-tube plantlets; (2) producing greenhouse hydroponic seedlings; and (3) breeding the virus-free potato seeds in the net room. Through combination of a specific rooting nutrient solution and a seedling strengthening nutrient solution and the method, virus-free potato seed supply can be completed within one year, seed property degradation can be effectively overcome, stress resistance is improved, excellent characteristics of varieties are reproduced, and the method has the advantages of being short in production period, low in production cost, large in propagation coefficient, flourishing in branches and leaves, developed in root system, high in survival rate, high in economic benefit and the like. The finally prepared detoxified sweet potato seeds are uniform in size, high in germination rate, consistent in growth vigor, stable in character and suitable for large-scale production.

The artificial compound feed is prepared from Peru fish meal, krill meal, squid meal, meat and bone meal, chicken meal, red earthworm powder, vital gluten, wheat flour, soybean meal, spirulina powder, spray-dried animal blood cells, sodium alginate, cholesterol, soybean lecithin, fish oil, astaxanthin, taurine, kieselguhr, choline chloride, ethoxyquin and mixed vitamins. The raw materials are scientifically proportioned to prepare the feed. According to the invention, the condition of tension supply of chilled fresh bait is effectively relieved, the feed is convenient to transport and store for a long time, and the breeding cost is reduced. The formula has the beneficial effects that the formula has a good food calling effect and a digestion promoting effect, and the immunocompetence and the anti-stress capability of the lobsters can be improved, so that the high survival rate and the high weight gain rate of the lobsters can be realized, and the yield of the lobsters is improved.

The invention discloses a preparation method and application of athlete-derived bifidobacterium probiotics for intestinal barrier repair, and relates to the technical field of biochemistry and microorganisms, and the preparation method comprises the steps of special bacteria source sampling, directional separation and purification, molecular biological identification, antibiotic sensitivity evaluation and injury repair verification. According to the preparation method and application of the sportsman-derived bifidobacterium probiotics for repairing the intestinal barrier, the sportsman intestinal flora is used as an exclusive bacterial source, and the unique metabolic elite attribute and stress adaptability of the sportsman intestinal flora are utilized to directionally screen and obtain a bifidobacterium strain adaptive to an extreme physiological stress environment; the bottleneck that conventional probiotics are only suitable for a common physiological environment and the survival rate and activity in a stress state are greatly reduced is broken through, high survival rate and metabolic activity can be kept in extreme environments such as exercise heat stress and the like, and accurate repair of intestinal barriers is realized through multiple mechanisms; meanwhile, an exclusive screening, identification and safety evaluation system is established, so that the safety and functional specificity of the strain are ensured.

The application discloses a functional probiotic microencapsulated intestinal shield system and a preparation method and application thereof. A core layer comprises four probiotics, i.e., lactobacillus acidophilus La-14, animal bifidobacterium Bb-12, lactobacillus rhamnosus GG and lactobacillus reuteri DSM 17938, and the weight ratio is 3:4:2:1, and the total viable bacterial count is greater than or equal to 10^10 CFU / g. An inner layer is a targeted release control layer, which is composed of ethyl cellulose, polylactic acid, chitosan and lactose, can swell in a pH 5.5 to 6.5 environment and can dissolve in a pH 6.8 to 7.2 environment. A middle layer is a buffer protection layer, which is composed of calcium carbonate, disodium hydrogen phosphate and sodium alginate, and can maintain the internal pH of the microcapsule at 4.5 to 5.5 in a gastric acid environment. An outer layer is an intelligent response protection layer, which is composed of konjac glucomannan, xanthan gum and gum arabic, and can form a protective film in a gastric acid environment with a pH less than 3.0 within 5 minutes. Through the synergistic protection of the four-layer microcapsule structure, the probiotics can achieve a high survival rate in the gastric acid environment of cats, and can be accurately released at specific positions in the intestinal tract, which is helpful to improve the intestinal health of cats.

The invention relates to the technical field of plant cultivation, in particular to a high-survival-rate seedling transplanting method. The transplanting method comprises the following steps: classifying and screening nursery stocks to obtain screened nursery stocks; lifting the screened seedlings according to a preset soil ball diameter to obtain a seedling transport body; loading the nursery stock transportation body into a container containing a pretreated nursery substrate (perlite, dried chicken manure, peat soil, straw and decomposed sweet potato seedlings) to obtain a nursery stock transplanting body; transferring the nursery stock transplanting body into the planting ditch for bed arrangement operation to obtain a nursery stock transplanting bed body; performing early-stage management on the nursery stock transplanting bed body to obtain a nursery stock bed; in the early-stage management, a compound organic fertilizer (rape seed cake, sheep manure, humic acid, wheat straw, bentonite, boric acid, magnesium sulfate, ammonium sulfate, calcium superphosphate, potassium sulfate and water) is used for additional fertilization; transplanting and planting the nursery stock bed to obtain transplanted nursery stocks. According to the method, by constructing a technical closed loop of root system microenvironment restoration, gradient adaptation and precise nutrition, the transplanting survival rate of the seedlings can be effectively increased.

The present invention provides an Escherichia coli strain resistant to nicotinamide mononucleotide stress and its application, which belongs to the field of microbial engineering technology. The strain is Escherichia coli ME59, which has been deposited in the China Center for Type Culture Collection on December 23, 2022, with a deposit number of CCTCC NO: M 20222060. The strain resistant to nicotinamide mononucleotide (NMN) stress provided by the present invention has both excellent anti-NMN performance and high NMN yield. On the one hand, under non-nicotinamide mononucleotide stress, strain ME59 has a higher NMN yield than the starting strain. On the other hand, strain ME59 overcomes the problem of low NMN tolerance of the starting strain, and can still maintain a high survival rate and NMN yield at high NMN concentrations, providing a high-yield NMN engineering strain.