39 results about "High survival rate" patented technology

The invention discloses a preparation method and application of athlete-derived bifidobacterium probiotics for intestinal barrier repair, and relates to the technical field of biochemistry and microorganisms, and the preparation method comprises the steps of special bacteria source sampling, directional separation and purification, molecular biological identification, antibiotic sensitivity evaluation and injury repair verification. According to the preparation method and application of the sportsman-derived bifidobacterium probiotics for repairing the intestinal barrier, the sportsman intestinal flora is used as an exclusive bacterial source, and the unique metabolic elite attribute and stress adaptability of the sportsman intestinal flora are utilized to directionally screen and obtain a bifidobacterium strain adaptive to an extreme physiological stress environment; the bottleneck that conventional probiotics are only suitable for a common physiological environment and the survival rate and activity in a stress state are greatly reduced is broken through, high survival rate and metabolic activity can be kept in extreme environments such as exercise heat stress and the like, and accurate repair of intestinal barriers is realized through multiple mechanisms; meanwhile, an exclusive screening, identification and safety evaluation system is established, so that the safety and functional specificity of the strain are ensured.

The application discloses a breeding method for improving the quality of Sebastes schlegelii fry, and comprises the following steps: collecting parent fish in a Sebastes schlegelii original seed field, artificially cultivating the parent fish in a net cage at sea, and performing nutrition rejuvenation on the parent fish; obtaining offspring fry by using an artificial fry squeezing method; controlling reasonable culture density according to different fry specifications; screening and using natural plant active ingredients and common micro-ecological preparations which are easy to absorb; avoiding disinfection and drug use; and controlling water flow rate in different growth stages. The method integrates nutrition rejuvenation, artificial fry production, pollution-free water quality regulation and fry behavior state regulation, solves problems such as poor parent germplasm, dead fry or insufficient activity of fry, slow growth and poor stress resistance in the process of fry breeding, has strong controllability in the operation period, has high survival rate of fry, has remarkable growth promotion and immunity effect, has good prevention and treatment effect on bacterial diseases, realizes pollution-free fry breeding, can reduce disease occurrence and mortality, reduces the feed conversion ratio, and effectively improves the economic benefit of breeding.

This invention relates to the field of microbial fermentation technology, specifically to a Monascus purpureus strain with strong stress resistance and high esterase production, and its applications. The Monascus purpureus strain is *Monascus purpureus* (…). Monascus ruber ZH01, deposited on October 20, 2025, at the China Center for Type Culture Collection (CCTCC), accession number: CCTCC NO:M 20252263. This *Monascus purpureus* strain exhibits strong resistance to adverse conditions and can grow normally in an environment with pH 3 and 25% ethanol. Its fermentation products, after being dried at 50°C for 24 hours, still maintain a higher survival rate compared to the control. Furthermore, it can produce high levels of esterifying enzymes in mixed culture media, with an esterification power of 165.8 mg / g·100h. During the fermentation of baijiu and vinegar, esterification generates lipids, enhancing the flavor of the beverages. The production of esterified red yeast rice using *Monascus purpureus* ZH01 of this invention has advantages such as short production cycle, low cost, and high esterification power.

This invention discloses a major gene for controlling cold tolerance in rice. bHLH150 This invention relates to the field of plant genetic engineering technology, specifically the major gene for controlling cold tolerance. bHLH150 The nucleotide sequence is shown in SEQ ID No:1. After CRISPR / Cas9 editing mutations in rice, the transgenic plants exhibited significantly reduced cold tolerance and became cold-sensitive; while those expressing or overexpressing the gene... bHLH150 The rice exhibits a high survival rate at low temperatures, thus improving its cold resistance. This invention relates to the major cold-resistance gene. bHLH150 Research on the molecular mechanisms regulating cold tolerance in rice is of significant theoretical and practical importance for the breeding of new cold-tolerant rice varieties. It also has broad application prospects and a promising market outlook in the agricultural field.

This invention relates to the field of plant tissue culture and the breeding of superior varieties of traditional Chinese medicinal materials. This invention discloses a method for rapid propagation of Epimedium inflorescences via tissue culture, comprising the following steps: S1, collecting newly sprouted flower inflorescences of Epimedium in early spring, disinfecting them, and setting them aside; S2, cutting the disinfected inflorescences into 1.1-1.2 cm segments, inoculating them into callus induction medium, and culturing them under conditions of 1200-1300 lux light intensity, 12 h / day photoperiod, and 24℃. This invention ensures uniform seedling quality and stable traits through targeted screening of effective components and monitoring of genetic stability. The optimized culture medium ratios at each stage result in a high proliferation coefficient, robust rooting, and a short propagation cycle, enabling efficient year-round propagation. Standardized seedling hardening and transplanting lead to a high survival rate. This method is technically stable and highly applicable, enabling the mass production of high-flavonoid glycoside, genetically stable, high-quality seedlings, fundamentally solving problems such as germplasm degradation, low propagation coefficient, and uneven quality, providing strong support for the breeding and industrialization of superior varieties of Epimedium.

The present invention belongs to the technical field of microorganisms, and particularly relates to a Lactobacillus plantarum NXU0011 and application thereof. The Lactobacillus plantarum NXU0011 is preserved in the China General Microbiological Culture Collection Center, with the preservation number of CGMCC NO: 26970. The bacterium has a good inhibition effect on Escherichia coli and Staphylococcus aureus. The Lactobacillus plantarum NXU0011 also has good antioxidant performance, and can be used for preparing hypoglycaemic drugs. At the same time, the Lactobacillus plantarum NXU0011 has a high survival rate in saliva, gastric juice or intestinal juice, and has the potential to become a good probiotic effect regulator.

This invention relates to a freeze-dried fermented mare's milk powder and its preparation method, belonging to the field of food processing technology. The preparation method of the freeze-dried fermented mare's milk powder includes the following steps: filtering, sterilizing, and cooling fresh mare's milk to obtain pretreated fresh mare's milk; adding a compound microbial agent to the pretreated fresh mare's milk for fermentation to obtain fermented mare's milk broth; concentrating the fermented mare's milk broth to obtain fermented mare's milk paste; adding a freeze-drying protectant to the fermented mare's milk paste; pre-cooling and freeze-drying to obtain the freeze-dried fermented mare's milk powder. This invention, through the compounding of trehalose, tremella polysaccharide, mannitol, glycine, grape seed polyphenols, and metal ions, results in a high survival rate of microorganisms in the freeze-dried fermented mare's milk powder.

This invention relates to the field of artificial breeding technology for natural enemy insects, and in particular to a method for the artificial breeding of the assassin bug *Triplophysa pulcherrima*. The invention employs steps including seed source collection and screening, rearing environment control, feed supply and management, mating and reproduction management, oviposition and egg mass collection, nymph hatching and age-differentiated rearing, overwintering management, and cannibalism control. By providing specialized rearing management devices, temperature and humidity conditions, standardized feed feeding, age-differentiated rearing density control, and moisture-controlled egg mass hatching, it solves the problems of high mortality rates among young nymphs, unstable mating success rates, low egg mass hatching rates, and the lack of a year-round breeding system in existing technologies. The method of this invention is simple to operate, has a high survival rate (nymphal survival rate exceeding 85%), an egg mass hatching rate exceeding 90%, and can achieve 4-5 generations per year, enabling large-scale, year-round production of *Triplophysa pulcherrima*, providing high-quality natural enemy resources for the biological control of agricultural and forestry pests.

This invention discloses a lactic acid bacterium and its application, belonging to the field of microbial fermentation technology. The lactic acid bacterium is *Lactobacillus plantarum* (Lactobacillus plantarum). Lactiplantibacillus plantarum FYcg1, with preservation number CGMCC No. 33190, is a strain that produces high levels of acid, is resistant to acid, salt, and bile salts, and exhibits a nitrite degradation rate exceeding 99%. It also shows significant inhibitory effects on Escherichia coli and Staphylococcus aureus. This invention optimizes the preparation method of a direct-inoculation starter culture, using skim milk powder, sucrose, and trehalose as a composite protective agent. The starter culture is prepared through specific centrifugation, pre-freezing, and vacuum freeze-drying, resulting in a high survival rate of live bacteria and good storage stability. When used for fruit and vegetable fermentation, it can rapidly lower pH, increase total acid content, and optimize flavor and metabolite composition, effectively solving problems such as long natural fermentation cycles, uneven quality, and poor safety. It is suitable for the industrialized and standardized production of pickled bamboo shoots.

This invention discloses a strain of *Lactococcus gasseri* LG3092 that produces the bacteriocin GarQ and effectively antagonizes multidrug-resistant *Helicobacter pylori*, and its applications. *Lactococcus gasseri* LG3092 was deposited at the Guangdong Provincial Microbial Culture Collection Center on October 30, 2023, with accession number GDMCC No: 63939. Studies have shown that LG3092 has the following beneficial effects: (1) it can produce the bacteriocin GarQ, effectively inhibiting the growth of *Helicobacter pylori* and other common pathogenic bacteria; (2) it has a high survival rate in the stomach, can colonize the stomach, and can compete with pathogenic bacteria for binding sites, effectively reducing the infection rate of *Helicobacter pylori* in the stomach; (3) it can improve the pathological state of the stomach and alleviate inflammation. Therefore, *Lactococcus gasseri* LG3092 has great application potential in the preparation of products for the prevention or treatment of *H. pylori*, especially multidrug-resistant *H. pylori* infection.

This invention belongs to the field of plant tissue culture technology, and specifically relates to a rapid propagation method for the tissue culture of Japanese red maple, Mikawa Nishiki. This invention achieves high survival rates of tissue-cultured regenerated plants by screening the hormone components and ratios of the initiation, proliferation, and rooting media, as well as optimizing the hardening-off and transplanting techniques. The method can be carried out year-round under controlled conditions, with a short production cycle, meeting the needs of industrialized seedling production and large-scale production of superior Mikawa Nishiki varieties.

The application discloses a breeding method of a green-shell egg producing black-bone chicken with seven-features and yellow-feather and dual-purpose, which comprises the following steps: taking yellow-feather line Xuefeng black-bone chicken as a KH line material, and then performing subline pure line breeding to obtain yellow-feather male chickens as a first male parent; taking pure line yellow-feather Xuefeng black-bone chicken as an H line material, and then performing subline pure line breeding to obtain yellow-feather female chickens as a first female parent; performing cross breeding of the first male parent and the first female parent to obtain a terminal male parent F1; taking yellow-feather pure line green-shell egg producing Xuefeng black-bone chicken as a LH line material, and then performing subline pure line breeding to obtain yellow-feather green-shell egg producing female chickens as a terminal female parent; and performing cross breeding of the terminal male parent and the terminal female parent to obtain a commercial generation green-shell egg producing black-bone chicken with seven-features and yellow-feather and dual-purpose. The black-bone chicken obtained by the breeding method has the basic features, flavor and nutritional value of the local pure breed Xuefeng black-bone chicken, can produce green-shell eggs, has a high egg production rate, has a fast growth speed, has good meat performance, has a high survival rate, and the like.

There is provided a microbial composition comprising Paenibacillus cineris and Micrococcus sp. for enhancing the inoculation rate of T. matsutake mycelium in trees, as well as a use thereof. The microbial composition according to the present invention significantly increases the inoculation rate of T. matsutake mycelium in trees. Furthermore, the composition does not inhibit the growth of T. matsutake mycelium and effectively suppresses harmful microorganisms that interfere with mycelium growth. The microbial composition of the present invention also remarkably improves the survival rate of T. matsutake-inoculated seedlings. According to the method for producing T. matsutake mycelium-inoculated seedlings of the present invention, seedlings with a high inoculation rate of T. matsutake mycelium and a high survival rate can be mass-produced and supplied in large quantities to mushroom cultivators.

The utility model discloses a kind of seedling protection cups suitable for passion fruit, fructus momordicae and other crops, it is related to agricultural seedling protection technical field, including cup body, the lower half part of cup body outer wall is covered with silver film, the top of the cup body is provided with ventilation component, detachable assembly is arranged between the ventilation component and the cup body, detachable connection is realized between the ventilation component and the cup body by detachable assembly, the top of the cup body can be changed to two kinds of state of ventilation / airtight by the ventilation component.The beneficial effects of the utility model are that: by ventilation component state switching, greenhouse seedling→progressive seedling→natural exposure high survival rate seedling is realized, the problem of insufficient seedling in traditional seedling, low survival rate of transplanting is solved, especially suitable for large-scale seedling and environment variable open-air planting scene.

The application provides a healthy human umbilical cord blood-derived immortalized NK cell line SZ093 and a preparation method and application thereof. The healthy human umbilical cord blood-derived immortalized NK cell line SZ093 is preserved in the Guangdong Provincial Microbial Culture Collection Center on September 26, 2025, and the preservation number is GDMCC No: 67036. The healthy human umbilical cord blood-derived immortalized NK cell line SZ093 provided by the application can still maintain good proliferation ability and high survival rate in a long-time (more than 1 year) culture process, and has high killing ability to tumor cells.

This invention relates to the field of environmental microbiology technology, and discloses a rapid aerobic degradation compound microbial agent for use in ecological public toilets under overload conditions, its preparation method, and its application. The compound microbial agent is composed of component A and component B at a live bacteria ratio of 1:1-3. Component A includes a lipolytic strain of *Pichia pastoris* FJQC-XJ-4 (CICC33293) acclimated under high organic load gradient conditions; component B includes a bacteriocin-producing strain of *Lactobacillus plantarum* bio-61663 (CICC24435). Under simulated overload conditions, the compound microbial agent achieves an organic matter degradation rate of 71.8% and a fecal coliform kill rate of 99.8%. The agent exhibits a high survival rate after 180 days of storage at room temperature and is suitable for emergency treatment of excessive sewage loads in scenic areas, highway service areas, and other locations during holidays.

This invention discloses a flexible clamping and positioning seedling delivery device for mechanical transplanting of naked rapeseed seedlings. It includes a seedling delivery frame, several horizontal feeding mechanisms, and a vertical flexible clamping and delivery mechanism. The horizontal feeding mechanisms are horizontally and symmetrically mounted on the seedling delivery frame, while the vertical flexible clamping and delivery mechanisms are mounted on the seedling delivery frame below the horizontal feeding mechanisms. This invention effectively performs posture changes and fixed-position seedling placement for naked rapeseed seedlings. During the clamping and delivery process, damage to the seedlings is minimal, ensuring a high survival rate after transplanting.

This invention relates to the field of plant genetic engineering technology, specifically disclosing a major gene for cold tolerance in rice seedlings, its encoded protein, and its applications. The major gene... RGA4L The nucleotide sequence is shown in SEQ ID No:1. This invention relates to a major gene controlling cold tolerance in rice seedlings. RGA4L After the expression of the gene was reduced in rice, the T3 transgenic plants showed a significant decrease in cold tolerance and became cold-sensitive; the gene was expressed or overexpressed. RGA4L Rice varieties with this gene exhibit high survival rates at low temperatures and demonstrate cold tolerance. Research on the molecular mechanism regulating cold tolerance in rice seedlings has significant theoretical and practical implications for the breeding of new cold-tolerant rice varieties. It also has broad application potential and market prospects in the agricultural field.

This invention discloses a method for the propagation and renewal of *Avicennia marina* germplasm resources. *Avicennia marina* is grafted onto radish rootstock to achieve propagation and renewal. This one-step tissue culture regeneration method eliminates the need for explant cutting, complex culture media, the addition of exogenous hormones, and multiple culture media changes, saving manpower and financial resources and preventing contamination risks during culture media changes. Compared with existing technologies, this root-transplantation cultivation method effectively solves the problem of *Avicennia marina* roots being unsuitable for soil, leading to early plant death and hindering propagation and renewal. Furthermore, utilizing radish roots, which are highly resistant to clubroot disease, solves the problem of clubroot disease and even plant death. In addition, radish roots can be harvested and stored for overwintering, maintaining a very high survival rate the following year. Compared to other cruciferous crops such as cabbage and mustard, using radish as a root donor facilitates overwintering and the subsequent greening and reproductive growth of *Avicennia marina* the following year.

The application discloses an EcN@BEVs probiotic compound and a preparation method and application thereof, and belongs to the technical field of biotechnology. The EcN@BEVs probiotic compound comprises Escherichia coli Nissle 1917 EcN and bletilla striata extracellular vesicles BEVs attached to the outer surface of the Escherichia coli Nissle 1917 EcN. The application constructs an advanced targeted delivery system for probiotic preparations, successfully connects the EcN and the BEVs in a directional manner, and constructs an engineered probiotic compound EcN@BEVs. The nano-coated BEVs attached to the outer surface of the Escherichia coli Nissle 1917 EcN serve as a rigid barrier, enhances the resistance to gastric acid and bile salts, promotes long-term retention in the intestinal tract by improving adhesion to mucosal epithelium, and improves the survival rate of probiotics under various extreme conditions in the digestive tract, and effectively promotes the colonization ability of the probiotics in the intestinal tract. Due to the anti-inflammatory properties of the bletilla striata-derived nanoparticles and the high survival rate and strong intestinal adhesion ability of the encapsulated probiotics, the EcN@BEVs probiotic compound has a better treatment effect in treating intestinal inflammation than the uncoated EcN.

This utility model relates to the field of agricultural machinery and discloses a multifunctional agricultural seeder, including a body. Three hoppers are fixedly fitted inside the body. Four support plates are fixedly connected to the bottom of the body. A rotating shaft is rotatably connected to the inner side of each support plate, and a moving wheel is fixedly fitted to the outer side of the rotating shaft. Three plowshares are fixedly connected to the bottom of the body, and three seeding pipes are fixedly connected to the bottom of the body. The seeding pipes, plowshares, and hoppers correspond to each other. An intermittent uniform seeding mechanism is provided between the body, the three hoppers, and the rotating shaft on the left rear side. This utility model has the following advantages and effects: it can periodically and uniformly sow seeds, thereby effectively controlling the seed sowing interval and avoiding overly concentrated or sparse sowing that reduces the sowing effect. Furthermore, after planting, the seeds can be buried to prevent localized seed exposure and maintain a high survival rate.

This invention provides a method for planting mulberry trees under photovoltaic panels in karst dolomite areas, belonging to the field of mulberry planting technology. The method includes: applying a water-retaining agent to the planting hole, covering it with soil, planting the mulberry trees between the photovoltaic panels in the karst dolomite area, applying fertilizer for the first time, and then applying topdressing and pest and disease control 2-3 months after the first fertilization. The mulberry varieties include one or more of Fengchi, Guiyou 62, Yue Sang No. 5, Nong Sang No. 14, and Qiang Sang No. 5. This invention has found that mulberry trees grow vigorously, uniformly, with thick branches, strong lateral branch sprouting ability, thick, dark green, and flat leaves under photovoltaic panels in karst dolomite areas, and have a high survival rate, making them suitable for growth under photovoltaic panels in karst dolomite areas. This method achieves multiple goals: efficient utilization of land resources under photovoltaic panels, prevention of soil erosion, ecological environment restoration, and added value to the sericulture industry, promoting the coordinated development of the photovoltaic industry and ecological agriculture.

This invention relates to a rooting culture medium and tissue culture method for Rhizoma Cypripedium, belonging to the field of plant culture technology. The tissue culture method includes the following steps: obtaining explants, sterilizing them, transplanting them to a seedling culture medium for primary culture, subculturing, and then transplanting the subcultured explants to a rooting culture medium for rooting culture to obtain Rhizoma Cypripedium seedlings; the seedling culture medium and rooting culture medium are sugar-free media. This method enables the artificial culture of Rhizoma Cypripedium, with high rooting rate, high seedling establishment rate, and high survival rate of transplanted Rhizoma Cypripedium seedlings.

This application provides a method for cultivating bonsai grapes with fruit, comprising: selecting healthy fruiting mother branches of grapes and cutting them in half to form cuttings, wherein the cuttings are partially connected to the original fruiting mother branches; filling a substrate bag with rooting substrate and sealing it; the substrate bag is made of a material that allows plant roots to penetrate; the rooting substrate is composed of perlite, peat moss, water-absorbing resin, and red clay; inserting the base of the cutting into the substrate bag, so that the cut part of the cutting is covered by the rooting substrate; wrapping the connection between the cutting and the original branch with a flexible medium that has absorbed rooting agent solution; the flexible medium is used to slowly release rooting agent to induce the sprouting of aerial roots at the connection; performing rooting cultivation, and when the root system fills the substrate bag and the fruit is in the small fruit stage, cutting off the cuttings and transplanting them into a potted container to obtain the bonsai grapes with fruit. This method can achieve rapid shaping of bonsai grapes with fruit with high survival rate and low fruit drop rate, and has important practical value.

This invention discloses a method for the artificial breeding and selection of small plateau native ornamental fish, belonging to the field of aquaculture technology. The method includes: S1 broodstock domestication, S2 hormone-free spawning induction, S3 fertilized egg hatching, S4 graded initial domestication, and S5 juvenile fish selection. In S2, the broodstock are placed in a spawning induction device, where visual mirror stimulation is generated through a reflecting mirror. Combined with the synergistic effect of gradually increasing light intensity from 8 hours to 12 hours and water temperature from 16°C to 22°C, and the weak electric field generated by the bottom microelectrode array (intensity below the fish's perception threshold, waveform is sine or square wave), natural spawning is induced without the addition of exogenous hormones, achieving a spawning rate of over 87.5%. In S4, the fry are fed graded micro-particle feed in three stages, with particle sizes of 80-120μm, 120-160μm, and 160-200μm, respectively. The fry feeding rate reaches over 87%, and the survival rate at 35 days of age reaches over 78%. This invention enables the artificial breeding of native ornamental fish in the plateau with zero hormones, and is simple to operate with a high survival rate.

The present application belongs to the technical field of biological medicine, and particularly relates to a kit for efficient AAV infection of 3D organoids and an infection method. The present application first provides a kit for efficient AAV infection of organoids in vitro, which comprises a box body and reagents, wherein the reagents comprise a basic culture medium configured for 3D organoid culture, optional nutritional supplements for promoting growth of the 3D organoids, and an AAV infection special culture medium. The AAV infection special culture medium comprises a TLTD infection-promoting system and specific components. The present application can achieve efficient AAV in vitro infection of organoids, and the organoids maintain a relatively high survival rate. In addition, the kit provided by the present application is simple to assemble and operate, the infection system provided is stable and controllable, and is suitable for infection of 3D organoids of various sources.

The application discloses a method for knocking out a SlSWC6 gene to improve low-temperature resistance of tomatoes and application thereof, and belongs to the technical field of biotechnology. SlSWC6 The nucleotide sequence of the protein coding region of the gene is shown as SEQ ID NO:1. SlSWC6 The application can significantly improve the low-temperature stress resistance of tomatoes by knocking out the gene through a CRISPR / Cas9 gene knockout technology. SlSWC6 Low-temperature treatment experiment results show that the gene knockout strain has higher survival rate, lower relative electrolyte permeability and higher photochemical efficiency under low temperature, and it is confirmed that the gene plays a negative regulation role in the low-temperature response of tomatoes. The application not only provides important gene resources for cultivating new low-temperature resistant tomato varieties, but also lays a theoretical foundation for analyzing the low-temperature adversity response mechanism of tomatoes.

This invention discloses a strain of *Pediococcus pentosaceus* and its applications, belonging to the field of microbial technology. The strain is *Pediococcus pentosaceus* (…). Pediococcus acidilactici L27, with accession number CCTCC NO: M 20252576, has the following outstanding characteristics: (1) strong cholesterol-lowering ability in vitro; (2) excellent tolerance to the gastrointestinal environment, maintaining a high survival rate in simulated gastric juice and bile salt-containing environments; (3) able to produce broad-spectrum antibacterial substances mainly composed of organic acids, which can inhibit a variety of foodborne pathogens and have stable antibacterial properties; (4) possessing hydrophobic cell surface and co-aggregation ability with pathogens, showing potential for intestinal colonization competition; and (5) having no hemolytic activity and good safety. The L27 strain of this invention can be used to prepare drugs, fermented dairy products, probiotic preparations or feed additives with cholesterol-lowering and / or intestinal flora regulation functions, providing a core strain resource for the development of multifunctional, efficient and stable new probiotic products.