2798 results about "High survival rate" patented technology

The invention relates to a Paris polyphylla var. yunnanensis scale seedling culturing method. The method comprises the following steps of: selecting Paris polyphylla var. yunnanensis plants more than 10 years old and reserving seeds for planting; collecting mature seeds; washing the collected seeds to remove episperm; spreading and drying the seeds in a shade and ventilating place within 12 hours, wherein a temperature is maintained and an accumulation thickness is less than or equal to 6 cm; spreading the seeds uniformly and drying; filling the seeds into a ventilating container; storing the container in a shade environment with a suitable temperature, wherein the storage time is no more than 2 months; clearing impurities and residues in a seedling culturing field; fertilizing decayed farm manure and calcium superphosphate; plowing and raking soil; forming a furrow; spreading humus soil on the furrow surface; flatting the furrow surface; sowing in early June next year; taking the stored seeds, and after sterilizing, broadcasting the seeds uniformly according to the thickness of sowing; earthing after broadcasting; covering mantle on the furrow surface; irrigating sufficiently and performing seedling management. A method for drying the seeds of the Paris polyphylla var. yunnanensis in shade in an environment without direct sunlight is adopted, the dormancy of the seeds of the Paris polyphylla var. yunnanensis is broken, and the scale production of Paris polyphylla var. yunnanensis seedlings is realized, so that germchit guarantee is provided for large-area popularization and cultivation of the Paris polyphylla var. yunnanensis. The method has the characteristics of simple and easily-learned operation, high survival rate of germchit, low production cost, healthy and strong germchit, stable and reliable technology, high repeatability, and capacity of effectively solving the problem of scale seedling cultivation of the Paris polyphylla var. yunnanensis.

The invention provides a tie-to-tree dendrobium officinale cultivation method, comprising the steps of seedling tissue-culture breeding, tissue-culture seedling acclimatization and tie-to-tree cultivation of acclimatized seedlings. The step of tissue-culture seedling acclimatization comprises selection of bottled tissue-culture seedlings, selection and arrangement of a seedling acclimatization medium, selection of seedling acclimatization fertilizer, planting and after-planting management and is characterized in that the seedling acclimatization medium consists of hardwood sawdust and broken stone mixed in a mass ratio of 6.5-7.5:2.5-3.5, and the seedling acclimatization fertilizer is organic fertilizer prepared by fermenting herbivorous animal waste. The step of tie-to-tree cultivation of acclimatized seedlings comprises selection of acclimatized seedlings, selection of cultivation forest land, selection of variety of trees to be tied, seedling tying up and planting management. The tie-to-tree dendrobium officinale cultivation method provided by the invention has the advantages of low planting cost, easiness in management, high survival rate and long harvesting period; moreover, the harvest products have high quality.

The invention relates to a planting method of Dendrobium officinale, and belongs to the technical field of plant cultivation. The invention provides a planting method of Dendrobium officinale which has high survival rate after transplanting tissue culture seedlings, rapid growth, high output, high medicinal value, little pesticide application and fewer pests, and the nutrition ingredients of the Dendrobium officinale are close to those of the wild Dendrobium officinale. The method comprises the following steps of: A, preparing a seed bed; B, preparing a flowerpot; C, field planting; D, watering; E, fertilizing; F, pest controlling and preventing; and G, controlling temperature and humidity. By the method, the survival rate of the Dendrobium officinale can be achieved to above 98%; and the incidence rate of insect pests can be obviously reduced. Through spraying by biogas slurry, the leaf stem of the Dendrobium is fleshy; the nutrition ingredient content is high; the incidence rate of insect pests can be reduced; the pesticide usage amount is reduced; the cost is reduced; and the food safety is improved. The Dendrobium officinale planted by taking the processed sheep manures and rapeseed meal as the top application has higher nutrition ingredient content and better medicinal effect; and the Dendrobium officinale can be harvested after 18 months.

The invention discloses a three-dimensional original ecological cultivation method for dendrobium candidum in forest. The three-dimensional original ecological cultivation method comprises the following steps of: firstly, selecting a cultivation environment; secondly, preparing a cultivation pot; thirdly, paving a matrix in the pot; fourthly, selecting seedlings; fifthly, permanently planting; sixthly, planting in a hanging manner or binding the seedling on the tree; seventhly, managing the cultivation; and eighthly, harvesting. The three-dimensional original ecological cultivation method disclosed by the invention provides a three-dimensional environment of original forest; the healthy growth of the dendrobium candidum is ensured; and the industrial and scale development of the dendrobium candidum can be realized; and the three-dimensional original ecological cultivation method disclosed by the invention has the advantages of high survival rate of seedlings, high yield, high quality of products, low cultivation cost and simpleness for management and operation.

The invention relates to a method for embedding a side-direction soil pressure sensor in the soil and a device thereof, which belongs to the technical field of construction engineering. The device comprises a sensor installation pipe, an extension pipe, a soil pressure sensor, a protective steel jacket, a U-shaped protective steel clip, a rigging screw, a connector pipe, a data transmission guide wire and a data collection instrument. The data transmission guide wire of the soil pressure sensor is connected with the data collection instrument. A notch is arranged in the center of the sensor installation pipe; the soil pressure sensor is put into the protective steel jack and then the soil pressure sensor and the protective steel jack are embedded in the notch; the U-shaped protective steel clip and the rigging screw are used to fix; the connector pipe is used to extend the sensor installation pipe and the extension pie to the design length; the pipes are put into a soil drilling hole; the guide pipes are fixed temporarily, and then fine sand is backfilled into the drilling hole; after the fine sand is consolidated, and the side direction soil pressure test is carried out. The invention has the advantages of convenient installation and operation, economy and practicality, high survival rate of the sensor embedded, capability of quite accurately measuring the side direction soil pressure in the soil, and accurate and reliable test result.

The invention discloses a method for segregated early weaning of piglets, which comprises the steps of medicine adding, segregation, early transfer and the feeding of early weaning by piglet feed, wherein the medicine adding is performed before and after the delivery of a sow, the advanced medicine adding is used to kill off microorganisms which is expected to be killed, and the medicine adding is also performed from the birth of a suckling piglet to the weaning; the segregation adopts that the parturient sow is transferred into a clean farrowing house for delivery from the pregnancy, and the piglet at the age of between 10 and 14 days is transferred into a clean and comfortable breeding house from the farrowing house; when the level of a maternal antibody of the piglet at the age of between 10 and 14 days is in higher state, the piglet is moved away from the sow and is transferred into a cleaner environment; and the piglet feed for segregated early weaning adopts four stages to feed. The piglet has early weaning segregation time, high survival rate, and quick growth speed, the segregated early weaning of the piglet can save feeding cost of the sow, improve the yearly parity number of the sow, improve the utilization rate of the farrowing house, and reduce the probability that the piglet is infected with sow diseases, and a segregated early weaning technology of the piglet is suitable for the application of modern pig farms.

The invention relates to a marine-fish environmental protective compound feed, consisting of the following materials in weight ratio: 13 to 20 portions of domestic fish powder, 8 to 15 portions of biological fish meal ABP600, 8 to 12 portions of soybean meal, 10 to 18 portions of peanut bran, 3 to 10 portions of corn, 3 to 7 portions of cotton seed meal, 3 to 7 portions of rapeseeds meal, 3 to 5 portions of meat and bone meal, 3 to 5 portionsof squid bowels powder, 20 to 30 portions of high gluten flour, 1.0 to 3.0 portions of blood meal, 1.0 to 2.0 portions of soybean lecithin, 2.0 to 3.0 portions of marine-fish oil, 0.3 to 0.6 portions of choline chloride (50%), 0.05 to 0.1 portions of Vitamin C phosphate (35%), 0.15 to 1.5 portions of calcium biphosphate, 0.2 to 0.5 portions of compound multiplex vitamin, 0.5 to 1.5 portions of compound mineral salt, 0.1 to 0.3 portions of feed attractant, 0.1 to 0.3 portions of micro-bio-preparation, 0.03 to 0.08 portions of A3Alpha peptidoglycan, 0.01 to 0.04 portions of phytase, and 0.04 to 0.08 portions of non-starch polysaccharase. The compound foodstuff can meet the nutrition demands of such high density cultivated marine fishes such as weever, large yellow croaker, American red fish, sparidae fishes, and trachinotus ovatus and rudd etc, and the cultivated objects can rapidly grow and has high foodstuff conversion rate; the compound foodstuff can intensify the immunity and anti-stress ability of fishes and endow them with good vitality, vivid body color and high survival rate; compared with marine fished cultivated by other foodstuffs, the excretion rates of nitrogen and phosphorous of the marine fishes cultivated by the compound foodstuffs is lowered by 10 to 15 percent, and cause smaller contamination to the cultivation water body.

The invention specifically relates to a bifidobacterium microcapsule and a preparing method thereof. The bifidobacterium is obligatorily anaerobic and very sensitive to oxygen, PH, temperature, humidity, and other adverse external environment, thereby being very hard to remain activity during production, storage and transport; besides, if taken orally in a form of dry bifidobacterium powder, the bifidobacterium cannot tolerate low-pH value gastric acid, bile salt, and other environments, so a purpose that massive survived bifidobacteria arrive at an intestinal tract and colonise on the intestinal mucosa is hard to be guaranteed. The preparing method disclosed by the invention comprises the following steps of: adding freeze-dried bifidobacterium powder into a sodium alginate solution and then mixing in soybean oil, emulsifying the mixed solution and standing; centrifugally collecting micro-capsules; and drying the micro-capsules by using a vacuum freeze-drying technology. The bifidobacterium microcapsule disclosed by the invention has the advantages of gastric acid resistance, bile salt resistance and entericsolubility, high survival rate of bifidobacterium, simple preparation method, strong practicality, convenience for industrial production, and excellent storage stability and solves the problem of short storage period of probiotics preparations.

The invention belongs to the technical field of microbial fermentation, specifically relates to a method for preparing an oil cake protein feedstuff through low moisture solid state fermentation. The method for preparing the oil cake protein feedstuff through the low moisture solid state fermentation is characterized in that: the method comprises the following steps: 1) preparing a bacterial inoculum: injecting a mixed solution (comprising a bacterial liquid and a sodium alginate solution) in a CaCl2 solution through an injector, washing three times through sterilized physiological saline after curing to obtain a microcapsule bacterial inoculum, wherein a volume ratio of the sodium alginate solution to the CaCl2 solution is 1:2-1:10; 2) mixing and fermenting cake base materials: uniformly mixing and stirring the microcapsule bacterial inoculum, oil cake and water, carrying out standing fermentation for 24-96 hours at a room temperature to obtain a fermented product; 3) adopting one of the following two methods: (1) carrying out drying, wherein the fermented product is dried after completing the fermentation to obtain the oil cake protein feedstuff; (2) directly carrying out vacuum packaging for the fermented product after completing the fermentation to obtain the oil cake protein feedstuff. The method has characteristics of high survival rate and low production cost.

The invention relates to aquaculture feed, in particular to litopenaeus vannamei compound feed special for winter shed cultivation and a preparation method of the feed. The feed comprises a protein source raw material, a fat source raw material, a sugar source raw material, an antioxidant, a phagostimulant, composite vitamin and composite mineral salt. The invention provides the litopenaeus vannamei compound feed special for winter shed cultivation and the preparation method of the feed, with the advantages of high survival rate, high growth speed, high stability in water, high ingestion speed, convenience for use and low environmental pollution, so as to solve the problems of low growth speed, serious disease, low survival rate and the like of the litopenaeus vannamei during winter shed cultivation at low temperature and promote the healthy development of the litopenaeus vannamei cultivation industry.

A technology for industrialized processing and molding of a light matrix includes the steps of crushing agricultural and forest waste first, composting the waste with beneficial microbes, namely EM bacteria for fermentation, conducting rational sifting and matrix matching, using a light-matrix processing and molding machine to process the scattered matrix into an enteroid matrix container with a diameter of 35mm to 50mm, and then cutting the matrix container into small sections, namely matrix sections in the length of 80mm to 100mm, thereby suiting both the cultivation of eucalyptus grafts and the upper bag of the seedling container for tissue-culture rooting. The technology, which can be used for industrialized matrix production, greatly reduces labor intensity and protects ecological environment. The matrix, belonging to composite matrix, can be used for seeding, cuttage and transplant of plants and for the cultivation of other seedlings for greening purpose, with a high survival rate and robust root systems.

The invention discloses an organic microorganism-containing ecological fertilizer and a production method thereof. The ecological fertilizer consists of a three-layer structure, an inner layer is made of compound fertilizer granules, an urease inhibitor and nitrification inhibitor coating is used as a middle layer, an outer layer is made of an organic microorganism coating agent prepared by using an active bacteria packing method; and the production method comprises the steps of mixing and granulating, inhibitor coating, and organic microorganism coating. The production method disclosed by the invention is simple, and a prepared ecological fertilizer is complete in nutrients, ensures a high survival rate due to mixed bacterial manures, ensures long survival time of effective living bacteria, has sufficient inorganic nutrients, is convenient to apply, can avoid topdressing, and promotes the development of non-toxic and harmless fertilizers; and the ecological fertilizer has an obvious antagonism effect on continuous cropping pathogenic fungi, therefore, the proliferation of bacteria is effectively promoted, the micro-ecological environment of soil is significantly improved, and after the crops are harvested, the soil is fertile so that next-stubble crops can grow very well.

The invention discloses a packaging method for a sensitivity enhanced optical fiber grating temperature sensor, belongs to the technical field of sensors, and relates to a sensitivity enhanced packaging method of the optical fiber grating temperature sensor. The method is characterized in that the optical fiber grating is packaged into a metal capillary tube by applying prestress to the optical fiber grating and gluing two ends of the optical fiber grating, and the metal capillary tube packaged with the optical fiber grating is put into an outer stainless steel tube the internal diameter of which is greater than the external diameter of the metal capillary tube, then heat conducting silicone grease is filled between the metal capillary tube and the outer stainless steel tube, and finally the two ends of the outer stainless steel tube are connected with an optical fiber jacket or an optical fiber cable; and a fiber pigtail of the optical fiber grating must be ensured to be in a bent state during the packaging process. The beneficial effects of the packaging method is that the sensitivity enhanced optical fiber grating temperature manufactured by the packaging method has the advantages of high temperature sensitivity, rapid response speed, simple structure, reasonable design, high survival rate of the field installation and the like, and can be used for high-precision temperature measurement in such occasions as intense electromagnetic radiation, flammable and explosive occasions and the like.

The invention relates to a submerged plant breeding blanket and application thereof. The submerged plant breeding blanket disclosed by the invention is respectively composed of a carrier layer, a matrix layer and an adhesive layer from bottom to top, wherein the carrier layer is composed of palm and bamboo plant fiber; the matrix layer is composed of a growing matrix suitable for submerged plants to grow; and the adhesive layer is composed of a plant fiber net. The invention further provides a submerged vegetation transplantation technology which is characterized in that after being subjected to seedling culture, submerged plant seedling breeding embryoid bodies prepared by virtue of the breeding blanket are transplanted to the water bottom of an ecological restoration area for planting and restoring submerged vegetation of the ecological restoration area. Compared with the traditional submerged plant transplantation technology method, the submerged vegetation transplantation technology disclosed by the invention has the advantages of low transplantation cost, high speed, high survival rate, convenient technology, strong technical maneuverability, high community forming speed after transplantation, high breeding speed, good community stability and the like, and can be used for realizing the quick transplantation and the community construction and configuration of the submerged plants.

The invention relates to a method for rapidly obtaining a CRISPR/Cas9 (clustered regularly interspersed short palindromic repeats/Cas9) gene knockout stable cell line through monoclonal cell sorting and belongs to the technical field of genetic engineering and genetic modification. According to the method, a CRISPR/Cas9 system, single-cell sorting by a flow cytometer and fluorescent protein screening on an expression vector are combined, positive monoclonal cells can be obtained in short time, and gene knockout work efficiency of the cell line is greatly improved. Compared with a traditional cell line gene knockout method, the flow cytometer is utilized for single-cell sorting, on one hand, tedious work of antibiotic screening is omitted, so that a large quantity of single cells can be obtained in quite short time, and on the other hand, the condition that cells in each culture hole are single cells can be guaranteed and false positive rate is reduced. Sorting is performed 40-80 h after cell transfection, at the time point, the highest survival rate of the cells after sorting can be guaranteed, and the screening efficiency is improved accordingly.

A method for transplanting the big tree with bare root includes such steps as digging a tree, trimming the tree to be transplanted, transporting, preparing the conditions for transplanting, transplanting, post management, and draining water and preventing waterlogging. It features that the consumptions of nutrients, water and energy for the tree to be transplanted are decreased and the physiological function of its tissue, organs and root is restored as fast as possible. Its advantages are high survival rate and convenient transport.

The invention discloses a rapid propagation method of grape grafted seedlings. The winter grape hard branch is taken as a rootstock and a scion. Through an appropriate temperature, the graft union is firstly in thermal synthesis. Afterwards the rootstock surface is heated for the callus of the graft union. A medicament with an proper formula is adopted for the dipping of the injured face to facilitate the healing of the graft union and the wound healing and rooting of the rootstock lower part, in which the step is that the first is the rootstock branch treatment, the second is the ear shoot treatment, the third is the inlaying grafting and connecting of the rootstock branch and ear shoot, as well as the ear opening bandaging, the fourth is the heat wound healing of the graft union, the fifth is the wound healing and rooting of the rootstock opening, the sixth is the transplanting and container transplanting seedling. The invention is simple and practical with convenient operation, short seedling cycle which only needs 1 to 8 months, high survival rate which can be more than 90%, low production cost, strong seedling growth, long growth period, fully aging branches and tendrils and good seedling quality, thereby the mechanical large-scale factory production is facilitated.

The invention provides a method of para position allocation and ,mixing cultivation of planting grass for afforestation on purple hills, which comprises the steps of: (1) division of land types: purple hilly lands are divided according to the acidity, basicity and lithology of the lands, soil erosion, soil thickness and terrain features; (2) selection of species of trees and grasses: species of trees and grasses which are applicable to grow in varied purple lands are selected according to the selection experiments of the different types of species of trees and grasses used for afforesting of purple hilly lands, and fitting degrees of the species of trees and grasses to each kind of land are specified; (3) para position allocation of the species of trees and grasses with the kinds of the lands: corresponding species of trees and grasses are allocated on the most suitable or relatively suitable lands; (4) mixed disposition of trees: different optimized disposition modes for purple hilly mixed forests are selected according to the mixed experiments of the trees for different kinds of lands of hills and hilly areas with purple soil, and trees and bushes, legumes and non-legumes, species of trees of deep root system and shallow root system, horizontally growing type and vertically growing type, soil fixing type and soil improved type are mixed. The method of the invention adopts trees, brushes and grasses to be planted on the most suitable and relatively suitable hills with purple soil, thus realizing precise para position allocation, high survival rate being up to over 90 percent and greatly accelerating the growing speed and forest establishment speed.

The invention provides a cuttage seedling culturing method for branch culture of honeysuckle. The method comprises the following steps: preparing branch culture nutrient solution; preparing a branch culture base material; cutting and picking cuttage branches; carrying out rooting culture; arranging a seedbed; carrying out cuttage; and carrying out seedling culture management. Compared with the prior art, the branch culture nutrient solution is adopted for rooting culture, and the branch culture base material is adopted, so that the survival rate of cuttage seedlings is 89 percent, and a cuttage seedling culturing method of honeysuckle with simple operation, high survival rate, strong stability and little investment is provided.

The invention relates to the field of livestock breeding technology, which specifically relates to a forage for sow during lactation period, and the forage is prepared by the following raw materials by weight: 70-80 parts of corn, 20-25 parts of bean cakes, 20-25 parts of millet bran, 15-18 parts of wheat bran, 12-15 parts of pea residues, 10-12 parts of dried white radish, 5-8 parts of black sesame, 3-4 parts of pig heat powder, 6-8 parts of vegetable sponge of luffa, 4-5 parts of fish meals, 2-3 parts of stone flour, 1-2 parts of shell powder, 5-6 parts of laver powder, 8-10 parts of purslane, 1-2 parts of radix Rhapontici, 2-3 parts of cape jasmine, 1-2 parts of pitch, 1-2 parts of Akebia stems, a proper amount of salts and 4-5 parts of feeding promoting agents. The prepared sow forage has balanced and comprehensive nutrition, and can satisfy each nutrition demand of postpartum sow, radix Rhapontici, cape jasmine, pitch, Akebia stems and other Chinese herbal medicine components can free mammary gland, increase milk yield, and the pigling fed by the sow which eats the forage has high survival rate, strong physique and red and bright hair.

The invention relates to a feed additive and a preparation method thereof, belonging to the technical field of feeds. The feed additive is characterized by comprising the following substances in percentage by weight: 40 percent to 60 percent of dogwood, astragalus, medlar and licorice extract, 30 percent to 50 percent of honeysuckle flower, cortex eucommiae, okra and mimosa pudica extract, 5 percent to 10 percent of fish meal, 0.5 percent to 1 percent of selenium yeast, 0.1 percent to 0.5 percent of compound vitamins, 0.1 percent to 0.2 percent of compound amino acids and 2 percent to 5 percent of spirulina. The feed additive has the advantages of rich raw material sources, low price, no toxic or side effects, effects of preventing various diseases of laying hens and improving immunity and disease resistance of the laying hens, environment-friendliness, high safety, high laying rate, high laying quality, high survival rate of the laying hens, enrichment of selenium, iodine, zinc and other nutrient elements, and the like.

The invention relates to a natural sea area large-scale ecological breeding method of stichopus japonicus, belonging to the field of aquiculture. The method can realize large-scale stichopus japonicus seedling breeding at the natural sea area by the steps of selecting and culturing parent stichopus japonicus, daily management of the parent stichopus japonicus, egg taking and incubating, culturing the young, culturing young stichopus japonicus, classifying offspring seeds and temporary culturing. The method solves the problems of degraded seedling quality, reduced production quality of the seedlings, gradual increment of stichopus japonicus diseases, high energy consumption of a seedling breeding room and the like in the process of seedling cultivation in the existing artificial seedling breeding room, overcomes the technical prejudice that sea cucumber seedlings can not be cultured at the natural sea area in the traditional view, breaks through the inherent thinking set, changes the traditional production way of the artificial seedling breeding room, and can realize ecological breeding in the natural sea area. Furthermore, the method has the remarkable advantages of high survival rate, no pollution and chemical dosing as well as being nuisanceless.

The invention discloses a liquid seaweed bio-fungus and a preparation process thereof. The liquid seaweed bio-fungus is compounded by the marine bio-fungus and three microbial floras including actinomycetes, lactobacillus and saccharomycetes. The process comprises the following steps: sterilizing the seaweeds at high temperature (over 100 DEG C), soaking, grinding and rubbing the seaweeds to prepare seaweed starch; proportionally mixing the seaweed starch with molasses, various auxiliaries, water and cultured fungus solution, stirring and then fermenting; and finally packaging. The preparation process of the invention is simple and is characterized by low production cost, high concentration and high survival rate. The liquid seaweed bio-fungus prepared by the process has the characteristics of high activity, quick absorption, high economic benefits and good social and environmental benefits.

A process based on fast shaping technique for preparing biological active artificial bone includes such steps as creating CAD 3D internal microstructure and external shape model of bone by CT data, fast shaping the microstructure and the negative mould of its external shape, implantating biological factors or cells, adding Ti fibres for reinforcing mechanism property, filling biological bone material, and preparing artificial substitute bone. Its advantages are high individual adaptibility, high preparing speed good biological compatibilility and high survival rate after implanlation.

An automatic indoor culture system for aquatic products features that the culture pool is stepped for staged cultures, the water for culture is treated by a circulating water treating system, the water quality is monitored and the optimum state is controlled by a water monitor system, and beside, the bait is uniformly distributed by an overlead moving throwing equipment. Its advantages are high density and quality, high survival rate and high output.