25 results about "Rotifer" patented technology

The invention provides a biological bait culture method for pond culture water. The biological bait culture method comprises the following steps: S1, building a three-dimensional culture system; s2, preparing an ecological base and a biocenosis; according to the method, by building a three-dimensional cultivation system of the bottom layer modular net cage, the water surface floating type three-dimensional cultivation frame and the intelligent regulation and control system and combining scientific configuration of ecological bases and biocenosis, the problem that bait supply is unstable in traditional cultivation is effectively solved; the light organism cultivation unit, the plant planting unit and the biological membrane carrier unit of the water surface floating type three-dimensional cultivation frame work in a layered mode, bait organisms such as chlorella, copepods and rotifers in different water layers are cultivated in a directional mode, a complete closed-loop food chain is constructed in addition to food filtering and nutrition supplementing of snails and freshwater mussels, and the bait yield per unit water body is greatly increased.

The invention provides a method for culturing Pseudosciaena crocea fries of the Halitium family through multi-stage dynamic bait density regulation and control, and belongs to the technical field of aquaculture. The culture method comprises the following steps: feeding 5-10 rotifers per mL at the age of 0-6 days; feeding 3-5 copepoda nauplii / mL and 0.5-1 artemia newly hatched larvae / mL at the age of 7-15 days; feeding 2-4 artemia nauplii / mL and 10-40% of compound feed at the age of 16-25 days, and gradually increasing the input proportion of the compound feed along with the age of days; 10%-80% of artemia and 20%-90% of compound feed are fed at the age of 26-35 days, and the input proportion of the compound feed is gradually increased along with the age of days; feeding mixed feed for 36-45 days old; photosynthetic bacteria and bacillus subtilis are put into the water body in the whole culture process. Through precise feeding and dynamic regulation and control, the survival rate, growth uniformity and feeding efficiency of the fries of the Pseudosciaena crocea of the sal family are effectively improved, and the quality of the fries is remarkably optimized.

The utility model relates to the technical field of rotifer harvesting device, specifically is a kind of rotifer screening device, it includes air pump and filter basket, the filter basket be equipped with screen, the outside of filter basket is fixed with the float pipe for suspending support filter basket in water body, the top surface of filter basket is fixedly connected with water outlet elbow, the upper end of water outlet elbow extends to filter basket, the lower end of water outlet elbow extends to the lower end of filter basket outside, and air pipe connector is arranged on water outlet elbow, and the lower end of water outlet elbow is connected with the suction pipe that extends into water body, and air stone is hung in suction pipe by inflation pipe, the top end of inflation pipe is connected with air pipe connector, the bottom end of inflation pipe is connected with air stone, and air pipe connector is connected with air pump by gas transmission pipe.The rotifer screening device can automatically screen the required size of rotifer, is easy to operate, and reduces labor intensity.

This invention relates to a method for the targeted cultivation of live feed to enhance the stress resistance of grouper fry, specifically a method for the targeted cultivation of grouper fry and its live feed. The live feed includes artichoke culture and rotifer culture. The targeted cultivation of the artichoke culture includes the following steps: placing artichokes in seawater, adding *Cryptospira spp.* powder, *Spirulina* powder, arginine, and *Cinnamomum camphora* oil for cultivation to obtain the artichoke culture; the targeted cultivation of the rotifer culture includes the following steps: placing rotifers in seawater, adding *Nyctaginus* pink bacteria. Natrialba pink Rotifer cultures were obtained by cultivating bacterial powder, spirulina powder, methionine, and sunflower oil. This method, through targeted cultivation of live feed, enriches the live feed with corresponding functional components. After feeding it to newly hatched juvenile scorpion, it can significantly improve the stress resistance of the juveniles and increase their survival rate, thus solving the industry pain points of slow development, low survival rate, and poor stress resistance of newly hatched scorpion scorpion.

The application discloses a kind of active sludge water quality state identification method based on rotifer population breeding, it is related to sewage treatment technical field, the method includes: collecting active sludge sample is placed in the culture device that can simulate aeration tank environment;In monitoring period, microscopic imaging is carried out to sample regularly, and time sequence image sequence is obtained;Time sequence image sequence is input into pre-training ViT model, and the type state, position and identity feature vector of rotifer individual are accurately identified and extracted;Based on feature vector, identity matching and continuous tracking of rotifer individual across time point are realized, and trajectory database containing whole life cycle trajectory is constructed;Based on the trajectory database, multiple characteristic parameters related to population breeding are calculated;These parameters are input into pre-trained water quality state classification model, and corresponding water quality state grade is automatically output.The method improves the accuracy and early warning timeliness of water quality state identification result significantly by fine-grained identification, individual tracking and multi-dimensional feature analysis.

The application discloses a disease prevention and multi-nutrition strengthening method for biological bait of blue crab breeding, which is applied to biological bait of the blue crab during the period from the nauplius to the zoea, and technical scheme points include disease prevention and multi-nutrition strengthening of rotifer and disease prevention and multi-nutrition strengthening of brine shrimp nauplius. Through multiple disinfection and multi-nutrition strengthening of the living biological bait, especially the rotifer and the brine shrimp nauplius, diseases of the crab seedlings caused by the biological bait with disease bacteria are prevented, and meanwhile, nutrition substances in the biological bait are specialized according to the growth and development needs of the crab seedlings, so that good growth and development of the crab seedlings are ensured.

The utility model relates to the technical field of freshwater fish breeding, and discloses a reservoir ecological freshwater fish rotifer preying device which comprises a material tank, a lower floating block for providing buoyancy is arranged at the bottom of the material tank, a motor is fixedly installed in the middle of the top side of the material tank, and six sets of discharging openings are formed in the bottom wall of the material tank in an array mode. A feeding assembly used for assisting in feeding rotifers is arranged in the material tank, a triggering assembly used for triggering and starting the feeding assembly is arranged in the middle of the supporting plate, and a photovoltaic panel used for providing power for all the components is arranged on the outer side of the material tank. Fish schools can be attracted to gather through the bionic bait, when the fish schools bite the bait, the guide rod can be driven to move downwards, so that the contacts make contact with one another, then the motor can be started, the gear disc rotates to drive the rotating rod to rotate, and then the spiral discharging rod is driven to rotate in the discharging opening to assist in rotifer throwing; therefore, fish schools can prey conveniently, and waste is avoided.

The invention discloses a circulating type seedling culture water quality regulation and control method based on weak electric field coupling multi-trophic-level organisms, and belongs to the technical field of seedling culture water quality regulation and control. The method comprises the following steps: putting chlorella with a certain density into a culture water body, culturing for a period of time, inoculating rotifers, and then culturing shellfishes in the culture water body to construct a rotifer-algae-shellfish multi-nutritional-level system; titanium electrode plates are placed at the two ends of the aquaculture water body and matched with a direct-current converter, air stones are laid at the bottom of the aquaculture water body, and a power source is switched on to form a weak electric field in the aquaculture water body; after fish and shellfish are bred and collected, the number of rotifers and chlorella in the breeding water body is adjusted to a certain extent according to the putting value, the rotifer-algae-shellfish multi-nutrition-level system is constructed, the aquaculture seedling breeding water body can achieve self-purification, the sewage treatment cost and the influence on the environment are reduced, and the breeding water body is more environmentally friendly. And the metabolic rate of each nutrition level in the multiple nutrition levels is increased through the weak electric field.

This application relates to the field of rotifer culture technology and discloses a method for regulating rotifer body size. This application successfully controls the body size of marine rotifers by regulating factors such as dissolved oxygen, temperature, salinity, and feed. Specifically, the conditions for large-scale rotifer culture include relatively low dissolved oxygen, temperature, and a larger feed, while the conditions for small-scale rotifer culture include relatively high dissolved oxygen, temperature, and a smaller feed. Certain cyanobacteria can also be used as supplementary feed. This application stably controls the environmental conditions for marine rotifer body size and selectively modifies the feed for the rotifers, achieving the goal of selectively regulating the body size of marine rotifers.

The present application relates to a kind of rotifer microflow water steady-state continuous expansion culture methods based on density-oviposition rate-ammonia nitrogen linkage control, suitable for the continuous supply of live bait in fry opening stage.The method is based on rotifer inoculation, microflow water change and a small amount of multiple feeding, and takes rotifer density, oviposition rate, total ammonia nitrogen and density variation coefficient as operation judgment index.When the total ammonia nitrogen in culture system increases, the oviposition rate decreases or the rotifer density fluctuates significantly in a short time, adjust the water change proportion, feeding frequency and harvesting proportion respectively.After the culture system reaches the preset steady-state condition, it is semi-continuous harvesting according to a certain proportion, and equal volume of new water and corresponding bait are added.The method can reduce the problems such as water quality deterioration, slow density recovery after harvesting and unstable continuous supply in fixed parameter culture.

The invention discloses an indoor cultivation method for increasing the survival rate of larvae and juveniles of horse mackerels, and relates to the technical field of aquaculture. Pretreated rotifers, shadberry fruit extracts and 2, 4 '-dihydroxychalcone are added in the early larva fish breeding stage and the later larva fish breeding stage, active ingredients such as gulcomannan, proline, chlorogenic acid and potentilla discolor extracts are added in the juvenile fish breeding stage, and meanwhile environmental factors such as illumination and sound wave stimulation are regulated and controlled, so that the juvenile fish breeding efficiency is improved. The survival rate of the horse mackerels and the capability of resisting infection of lactococcus gasseri and vibrio anguillarum can be effectively improved, the investment of the breeding cost is reduced, and the used baits are simple, easy to obtain and simple to prepare.

According to the technical scheme, the large yellow croaker large-scale breeding method suitable for mechanization is characterized in that seedling raising facilities are subjected to standardized transformation, for example, a water supply and drainage system and an inflation system of a seedling raising pond are optimized, special equipment such as an automatic bait casting machine and a dirt suction machine are integrated, and mechanical operation of key links such as bait casting and dirt suction is achieved. Meanwhile, by deploying a water quality on-line monitoring system and tracking key parameters such as dissolved oxygen and temperature in the whole process, operation standards such as water change, bait casting and dirt suction in each cultivation stage such as a rotifer stage, an artemia stage and a compound feed stage are quantified, and a digital management process is formed. According to the method, manpower dependence is effectively reduced, the standardization degree and efficiency of fry breeding operation are improved, and a solution is provided for quality improvement, efficiency improvement and transformation and upgrading of the large yellow croaker breeding industry.

The utility model discloses a rotifer ecological simulation breeding cabin, and relates to the technical field of fishery breeding devices. The top surface of the cabin body is hinged with a cabin cover; the inner bottom wall of the cabin body is provided with an electric heater; the top surface of the cabin cover is fixedly connected with a material storage barrel in a penetrating manner; the top surface of the material storage barrel is fixedly connected with a barrel cover; a stirring paddle is fixedly connected to the side wall of the lengthened shaft, a rotating ring is rotationally connected to the lower portion of the side wall of the discharging pipe, a gear ring is arranged on one side of the rotating ring, a protruding rod is fixedly connected to the side wall of the rotating ring, and a uniform distribution disc is fixedly connected to the end, away from the rotating ring, of the protruding rod; according to the rotifer feeding cabin, feed can be evenly distributed in the cabin body so as to avoid the situation that due to the fact that the feed is accumulated at one position, the local feeding amount is too large, the survival rate is improved, and it can be ensured that the water temperature is stabilized within a suitable range so that the survival of rotifers can be guaranteed.

The application provides a method for improving the disease resistance of Litopenaeus vannamei "Guangtai No.1", wherein (1) the water temperature is 28.3-28.7 DEG C when the Litopenaeus vannamei "Guangtai No.1" juvenile enters a breeding pond, and then is increased to 29.8-30.2 DEG C, and then is decreased to 28.8-29.2 DEG C when the M2 of Tachycaris sp. reaches, and is decreased to 27.8-28.2 DEG C when the P2 of the juvenile shrimp reaches; (2) the density is 180-200 thousand / m 3 ; (3) the feed: the metamorphosis of the nauplius into the copepodid is started, and the feed of Chateoceros muelleri and Skeletonema costatum is started, and the total density is kept as 400-600 pieces / ml; the microcapsule feed is supplemented when the copepodid is at the second stage, and the feeding frequency is 5-6 times per day; the Skeletonema costatum is fed when the copepodid is at the third stage, and the density is 90-110 pieces / ml; the microcapsule feed, shrimp slices and rotifer are fed when the Tachycaris sp. is at the first and second stages; the nauplius of Artemia is fed twice and the micro-particle feed is fed four times when the Tachycaris sp. is at the third stage; the nauplius of Artemia is fed four times and the micro-particle feed is fed twice when the juvenile shrimp is at the first stage. The application effectively improves the disease resistance of the shrimp fry, and improves the survival rate and the qualified rate.

The present application relates to the field of aquaculture technology, and particularly relates to a preparation method and application of seawater fry biological bait. The preparation method comprises the following steps: mixing Schizochytrium sp. powder, bile acid and sunflower phospholipid according to a mass ratio of 50:10-20:10-20 to obtain a nutrition enhancer; diluting the nutrition enhancer to a concentration of 40g / t-50g / t to obtain a diluent; adding 130-150 ordinary rotifers per milliliter of the diluent, and culturing for 12-18 hours to collect enhanced rotifers; adding 30-50 ordinary artemia per milliliter of the diluent, and culturing for 12-18 hours to collect enhanced artemia; and combining the enhanced rotifers and the enhanced artemia to obtain the fry biological bait. The fry biological bait prepared by the present application has uniform fry size and high survival rate during the bait conversion period, and has significant economic and ecological benefits.

The invention relates to a method for cryopreserving eggs, nauplii and young barnacles of barnacles. Furthermore, the present invention relates to the cryopreserved feed and the use of the preserved organisms as live feed organisms in marine aquaculture, typically in place of rotifers and artemia and other living feed organisms.

This invention relates to the field of aquaculture technology, specifically to a highly efficient and large-scale artificial breeding method for *Catfish bream*. The method includes a seedling cultivation step; the water in the seedling tank is obtained by treating fresh aquaculture water with fertilization, and the water in the seedling tank contains initial feed for *Catfish bream* fry; the initial feed includes rotifers and cladocerans. The fertilization method involves: after filling the seedling tank with fresh aquaculture water, sprinkling soybean milk, a dispersion of bacterial and algal nutrient paste, and a dispersion of algal polypeptides onto the water surface, and then adding rotifers to the water in the seedling tank. This technical solution can solve the technical problems of existing artificial breeding techniques for *Catfish bream*, which have many weaknesses and cannot meet the demands of modern aquaculture for efficient, stable, and high-quality seedling production. This technical solution has the advantages of improving breeding efficiency, ensuring seedling quality, adapting to large-scale production, reducing costs, and being environmentally friendly, making it suitable for efficient and large-scale artificial breeding of *Catfish bream*.

This invention provides a method for rapidly cultivating P5 shrimp larvae, comprising the following steps: transferring nauplii into a larval rearing pond for cultivation; after more than 80% of the nauplii have developed into zoea larvae, feeding them artificial feed for zoea larvae; after more than 80% of the zoea larvae have developed into mysid larvae, feeding them feed for Artemia nauplii and mysid larvae; after more than 80% of the mysid larvae have metamorphosed into shrimp larvae, feeding them rotifers and shrimp flakes; the artificial feed for zoea larvae includes: 45-55 parts of Schizochytrium powder, 15-27 parts of soy milk, 10-15 parts of egg yolk, and 15-20 parts of *Gnaphalium affine* oil. The artificial feed for mysid larvae includes: 40-44 parts of shrimp flakes, 12-16 parts of tubifex worm powder, 5-10 parts of mannan oligosaccharide, 10-14 parts of scabra oil, and 20-30 parts of *Eupatorium fortunei* oil. This invention shortens the development time while obtaining high-quality P5 shrimp larvae, and throughout the entire cultivation process, the shrimp larvae mortality rate is low and the seedling survival rate is high.

This invention relates to the field of marine organism rearing technology, specifically providing a method for the low-temperature breeding of moon jellyfish in artificial rearing. The method includes seawater preparation, collection of planktonic larvae, cultivation of polyps, and cultivation of adult jellyfish. The polyps are cultivated at a low temperature of 12-15℃ and fed with rotifers and nutrients. During the cultivation of adult jellyfish, *Lactobacillus plantarum* is used to assist in the cultivation. This method, through low-temperature cultivation and the addition of nutrients, improves the attachment rate of planktonic larvae, the survival rate of polyps and discoid bodies, and increases the size of adult jellyfish, significantly improving the breeding efficiency and quality of moon jellyfish.

The invention discloses an activated sludge water quality state recognition method based on rotifer population reproduction, and relates to the technical field of sewage treatment.The method comprises the steps that an activated sludge sample is collected and placed in a culture device capable of simulating an aeration tank environment; performing microscopic imaging on the sample regularly in the monitoring period to obtain a time sequence image sequence; inputting the time sequence image sequence into a pre-trained ViT model, and accurately identifying and extracting the type state, the position and the identity feature vector of the rotifer individual; based on the feature vectors, cross-time-point identity matching and continuous tracking of rotifer individuals are achieved, and a trajectory database containing full-life-cycle trajectories is constructed; calculating a plurality of feature parameters related to population reproduction based on the trajectory database; and inputting the parameters into a pre-trained water quality state classification model, and automatically outputting corresponding water quality state grades. According to the method, through fine-grained recognition, individual tracking and multi-dimensional feature analysis, the accuracy of a water quality state recognition result and the early warning timeliness are remarkably improved.

The application belongs to the technical field of biotechnology, and particularly relates to a cultivation method of obligate parthenogenetic rotifer based on a gene editing technology and application of the obligate parthenogenetic rotifer in preparation of novel water product breeding opening bait. The obligate parthenogenetic rotifer is obtained by combining new rotifer microinjection with screening of rotifer mutant through gene knockout, and the novel obligate parthenogenetic rotifer variety prepared by the application can be used as a novel living bait for water product breeding.

The present disclosure provides a system for producing rotifers, the system comprising a bacterial culture tank and a rotifer culture tank, wherein bacteria are cultured in the bacterial culture tank using an algal culture supernatant as feed, the cultured bacteria are supplied to the rotifer culture tank, and rotifers are cultured in the rotifer culture tank using the bacteria as feed.

This utility model discloses an integrated device for circulating water culture of rotifers, belonging to the field of rotifer farming equipment technology. The device includes a culture tank and a purification tank connected by pipes. The water inlet pipe of the culture tank includes a main water inlet pipe and multiple branch water inlet pipes connected to the culture tank. The outlets of the branch water inlet pipes are aligned with the interior of the culture tank. At least one slow-flow filter plate is installed below the outlet of each branch water inlet pipe. The slow-flow filter plate is connected to the culture tank and located above the water surface in the culture tank. The slow-flow filter plate slows down the inlet water flow rate so that the water forms a dripping flow when it flows out. In this device, the water is first diverted through the branch water inlet pipes. This diversion effectively reduces the impact force of the water flow, and the slow-flow filter plate slows down the water flow, forming a slow-flowing dripping water curtain. The combined effect of the branch water inlet pipes and the slow-flow filter plate slows down the water flow rate into the culture tank, preventing excessive water flow from affecting the growth and reproduction of the rotifers.

The utility model discloses a rotifer breeding pond which comprises a breeding pond body, the bottom of the breeding pond body is provided with a sedimentation part, the height of the sedimentation part is reduced from the periphery of the breeding pond body to the center of the breeding pond body, the bottom end of the sedimentation part is provided with a sewage draining exit, and the sewage draining exit is communicated with an external sewage draining device; the vortex generating device is arranged at the edge of the bottom of the cultivation pool and used for making vortexes for water flow in the cultivation pool. The sedimentation part inclining towards the center is arranged at the bottom of the breeding pond, the vortex generating device is arranged, in the rotifer breeding process, vortexes are generated through the vortex generating device, and under the guiding effect of the sedimentation part, waste such as residual feed and excrement is guided to the sewage draining exit, so that continuous flowing of water flow is kept, and dead corners of water are avoided; therefore, the distribution efficiency of dissolved oxygen and the removal efficiency of waste are improved, and the quality level of breeding is further improved.

The present invention relates to a method for high-density milkfish larval rearing comprising the steps of cleaning and chlorinating a culture tanks overnight; rinsing culture tanks and installing aeration lines and air stones; stocking a dechlorinated seawater; adding green algae (Nannochloropsis sp.) at a density of 1 X 106 cells / ml; stocking a milkfish larvae at stocking density of 60 to 120 milkfish larvae per liter; feeding the stocked milkfish larvae with rotifer, Artemia, and artificial diet; adding probiotics; partially changing the dechlorinated seawater daily; and employing flow-through system to the culture tanks between 6 PM to 8 AM starting on day 9 up to the harvest period of the milkfish larvae. By increasing larval stocking density, production capacity of the hatchery is increased, and disposal of milkfish eggs is minimized. The disclosed method improves the production cost and returns in milkfish hatchery operations without affecting the fry quality.