38 results about "Rotifer" patented technology

The invention provides an in-situ ecological treatment method for recycling prawn culture tail water and application of the in-situ ecological treatment method, and belongs to the technical field of aquaculture wastewater treatment. After the miscellaneous shrimps in the culture water body are cleaned, quick lime is splashed to be matched with an iron chain to drag the bottom, and then a composite treating agent is put; inoculating rotifer, and then supplementing mineral substances; performing water quality verification; the composite treatment agent comprises EM bacteria, a seawater chlorella stock solution and a carbon source. According to the method, in-situ ecological treatment of prawn culture tail water and a zero-drainage culture mode are achieved, the treatment period is short, the culture survival rate is high, new treatment facilities are not needed, and beneficial bacterial algal phases and nutrient elements can be reserved.

The invention relates to the technical field of aquaculture, in particular to a preparation method and application of seawater larval fish biological bait. The preparation method comprises the following steps: mixing schizochytrium limacinum powder, bile acid and sunflower phospholipid according to a mass ratio of 50: (10-20): (10-20) to obtain the nutrition enhancer; diluting the nutrition enhancer until the concentration is 40g / t-50g / t, so as to obtain a diluent; adding 130-150 common rotifers into each milliliter of diluent, culturing for 12-18 hours, and collecting strengthened rotifers; 30-50 common artemia is added into each milliliter of the diluent, culturing is carried out for 12-18 hours, and reinforced artemia is collected; the combination of the strengthened rotifer and the strengthened artemia is the larval fish biological bait. The larval fish biological bait prepared by the invention is uniform in fry specification and high in survival rate in a bait transfer period, and has remarkable economic and ecological benefits.

The invention discloses bacillus velezensis Bve1910, which is preserved in the China Center for Type Culture Collection (CCTCC) and has the preservation number of CCTCC NO. M 20221965. The strain and metabolites thereof have strong antagonistic effects on more than ten types of marine culture animal pathogenic bacteria such as vibrio harveyi, vibrio erwiniae, vibrio canbayi, vibrio parahaemolyticus, vibrio alginolyticus, vibrio anguillarum, vibrio cholerae, vibrio shewanii, vibrio rotifer, pseudoalteromonas and photobacterium damarae, are strong in antibacterial activity and wide in antibacterial spectrum, and can be used for preparing marine culture animal pathogenic bacteria. According to the preparation method, the traditional Chinese medicine composition is simple in preparation method and rich and stable in metabolite, an ammonium sulfate precipitation extract, a hydrochloric acid precipitation methanol extraction extract, an ethyl acetate extraction extract and a chloroform extraction extract all have a broad-spectrum bacteriostatic effect, functional substances playing the broad-spectrum antagonistic bacteriostatic effect are compounded and diversified, and the directional selection effect on bacteria in the environment and ecology is not generated; and pathogenic bacteria are not easy to generate tolerance, so that the method has good application safety and ecological safety properties.

The invention provides a biological bait culture method for pond culture water. The biological bait culture method comprises the following steps: S1, building a three-dimensional culture system; s2, preparing an ecological base and a biocenosis; according to the method, by building a three-dimensional cultivation system of the bottom layer modular net cage, the water surface floating type three-dimensional cultivation frame and the intelligent regulation and control system and combining scientific configuration of ecological bases and biocenosis, the problem that bait supply is unstable in traditional cultivation is effectively solved; the light organism cultivation unit, the plant planting unit and the biological membrane carrier unit of the water surface floating type three-dimensional cultivation frame work in a layered mode, bait organisms such as chlorella, copepods and rotifers in different water layers are cultivated in a directional mode, a complete closed-loop food chain is constructed in addition to food filtering and nutrition supplementing of snails and freshwater mussels, and the bait yield per unit water body is greatly increased.

The invention provides a method for culturing Pseudosciaena crocea fries of the Halitium family through multi-stage dynamic bait density regulation and control, and belongs to the technical field of aquaculture. The culture method comprises the following steps: feeding 5-10 rotifers per mL at the age of 0-6 days; feeding 3-5 copepoda nauplii / mL and 0.5-1 artemia newly hatched larvae / mL at the age of 7-15 days; feeding 2-4 artemia nauplii / mL and 10-40% of compound feed at the age of 16-25 days, and gradually increasing the input proportion of the compound feed along with the age of days; 10%-80% of artemia and 20%-90% of compound feed are fed at the age of 26-35 days, and the input proportion of the compound feed is gradually increased along with the age of days; feeding mixed feed for 36-45 days old; photosynthetic bacteria and bacillus subtilis are put into the water body in the whole culture process. Through precise feeding and dynamic regulation and control, the survival rate, growth uniformity and feeding efficiency of the fries of the Pseudosciaena crocea of the sal family are effectively improved, and the quality of the fries is remarkably optimized.

The utility model relates to the technical field of rotifer harvesting device, specifically is a kind of rotifer screening device, it includes air pump and filter basket, the filter basket be equipped with screen, the outside of filter basket is fixed with the float pipe for suspending support filter basket in water body, the top surface of filter basket is fixedly connected with water outlet elbow, the upper end of water outlet elbow extends to filter basket, the lower end of water outlet elbow extends to the lower end of filter basket outside, and air pipe connector is arranged on water outlet elbow, and the lower end of water outlet elbow is connected with the suction pipe that extends into water body, and air stone is hung in suction pipe by inflation pipe, the top end of inflation pipe is connected with air pipe connector, the bottom end of inflation pipe is connected with air stone, and air pipe connector is connected with air pump by gas transmission pipe.The rotifer screening device can automatically screen the required size of rotifer, is easy to operate, and reduces labor intensity.

This invention relates to a method for the targeted cultivation of live feed to enhance the stress resistance of grouper fry, specifically a method for the targeted cultivation of grouper fry and its live feed. The live feed includes artichoke culture and rotifer culture. The targeted cultivation of the artichoke culture includes the following steps: placing artichokes in seawater, adding *Cryptospira spp.* powder, *Spirulina* powder, arginine, and *Cinnamomum camphora* oil for cultivation to obtain the artichoke culture; the targeted cultivation of the rotifer culture includes the following steps: placing rotifers in seawater, adding *Nyctaginus* pink bacteria. Natrialba pink Rotifer cultures were obtained by cultivating bacterial powder, spirulina powder, methionine, and sunflower oil. This method, through targeted cultivation of live feed, enriches the live feed with corresponding functional components. After feeding it to newly hatched juvenile scorpion, it can significantly improve the stress resistance of the juveniles and increase their survival rate, thus solving the industry pain points of slow development, low survival rate, and poor stress resistance of newly hatched scorpion scorpion.

The present application relates to the field of microbial technology and discloses a yeast strain Pichia kudriavzevii and its application as well as a method for cultivating rotifers. The yeast strain Pichia kudriavzevii described in the present application is deposited with GDMCC No: 66038 and its Latin name is Pichia Kudryavzevii . The present application screened out a strain of Pichia kudriavzi from a seawater environment, which naturally has the ability to adapt to the high salinity of seawater, avoiding the problem that Bacillus subtilis and other conventional yeasts in the current freshwater environment cannot be used efficiently in rotifer culture and cause water pollution; at the same time, it has extremely significant culture efficiency when used as bait for rotifers, and can significantly increase the yield of rotifers in a shorter time than conventional Bacillus subtilis and other yeasts, so that rotifer culture can be carried out in a low-cost, high-efficiency, and green and environmentally friendly manner.

The application discloses a kind of active sludge water quality state identification method based on rotifer population breeding, it is related to sewage treatment technical field, the method includes: collecting active sludge sample is placed in the culture device that can simulate aeration tank environment;In monitoring period, microscopic imaging is carried out to sample regularly, and time sequence image sequence is obtained;Time sequence image sequence is input into pre-training ViT model, and the type state, position and identity feature vector of rotifer individual are accurately identified and extracted;Based on feature vector, identity matching and continuous tracking of rotifer individual across time point are realized, and trajectory database containing whole life cycle trajectory is constructed;Based on the trajectory database, multiple characteristic parameters related to population breeding are calculated;These parameters are input into pre-trained water quality state classification model, and corresponding water quality state grade is automatically output.The method improves the accuracy and early warning timeliness of water quality state identification result significantly by fine-grained identification, individual tracking and multi-dimensional feature analysis.

The invention provides a sea bass breeding method which comprises the following steps: parent fish selection and intensive cultivation: selecting 3-5-year-old healthy parent fish, domesticating in a circulating water system with illumination, water temperature and salinity, and feeding intensive feed to promote gonad development; step-by-step spawning induction: injecting the female fish twice, and injecting the male fish once to improve spawning induction synchronism; the semen and the eggs are mixed and activated through ultraviolet irradiation, and the fertilization efficiency is improved; pulse-type water flow hatching: inhibiting water mildew by combining with sound wave oscillation, wherein the hatching rate reaches 85% or above; staged fry breeding: phage-probiotic enhanced rotifers are fed in the fry stage, and microcapsule feed coated with sodium alginate-chitosan is used in the juvenile stage, so that the survival rate and the growth speed of the fry are remarkably increased. The method solves the problems of low fertilization rate, weak seedling disease resistance and the like in traditional breeding through methods of environment simulation, nutrient enrichment, biological prevention and control and the like, and is suitable for large-scale seedling breeding and deep-sea breeding.

The application discloses a disease prevention and multi-nutrition strengthening method for biological bait of blue crab breeding, which is applied to biological bait of the blue crab during the period from the nauplius to the zoea, and technical scheme points include disease prevention and multi-nutrition strengthening of rotifer and disease prevention and multi-nutrition strengthening of brine shrimp nauplius. Through multiple disinfection and multi-nutrition strengthening of the living biological bait, especially the rotifer and the brine shrimp nauplius, diseases of the crab seedlings caused by the biological bait with disease bacteria are prevented, and meanwhile, nutrition substances in the biological bait are specialized according to the growth and development needs of the crab seedlings, so that good growth and development of the crab seedlings are ensured.

The utility model relates to the technical field of freshwater fish breeding, and discloses a reservoir ecological freshwater fish rotifer preying device which comprises a material tank, a lower floating block for providing buoyancy is arranged at the bottom of the material tank, a motor is fixedly installed in the middle of the top side of the material tank, and six sets of discharging openings are formed in the bottom wall of the material tank in an array mode. A feeding assembly used for assisting in feeding rotifers is arranged in the material tank, a triggering assembly used for triggering and starting the feeding assembly is arranged in the middle of the supporting plate, and a photovoltaic panel used for providing power for all the components is arranged on the outer side of the material tank. Fish schools can be attracted to gather through the bionic bait, when the fish schools bite the bait, the guide rod can be driven to move downwards, so that the contacts make contact with one another, then the motor can be started, the gear disc rotates to drive the rotating rod to rotate, and then the spiral discharging rod is driven to rotate in the discharging opening to assist in rotifer throwing; therefore, fish schools can prey conveniently, and waste is avoided.

The invention discloses a circulating type seedling culture water quality regulation and control method based on weak electric field coupling multi-trophic-level organisms, and belongs to the technical field of seedling culture water quality regulation and control. The method comprises the following steps: putting chlorella with a certain density into a culture water body, culturing for a period of time, inoculating rotifers, and then culturing shellfishes in the culture water body to construct a rotifer-algae-shellfish multi-nutritional-level system; titanium electrode plates are placed at the two ends of the aquaculture water body and matched with a direct-current converter, air stones are laid at the bottom of the aquaculture water body, and a power source is switched on to form a weak electric field in the aquaculture water body; after fish and shellfish are bred and collected, the number of rotifers and chlorella in the breeding water body is adjusted to a certain extent according to the putting value, the rotifer-algae-shellfish multi-nutrition-level system is constructed, the aquaculture seedling breeding water body can achieve self-purification, the sewage treatment cost and the influence on the environment are reduced, and the breeding water body is more environmentally friendly. And the metabolic rate of each nutrition level in the multiple nutrition levels is increased through the weak electric field.

The present application belongs to the field of bioengineering, and particularly relates to a method for controlling pests in an open culture system of microalgae based on light-induced coupling electric inhibition. The main steps are as follows: a light irradiation device and a high-voltage discharge device are arranged on one side of a raceway pond; first, light-induced treatment is performed, and the raceway pond is irradiated at the same time, the irradiated light at the left curve and the right curve is blue light with an intensity of 1500-2000 Lux; the irradiated light at the upper raceway and the lower raceway is red light with an intensity of 1000-2000 Lux; so as to attract rotifers to the left curve and the right curve to form rotifer aggregation areas; then, the rotifer aggregation areas at the left curve and the right curve are subjected to electric shock treatment; the present application can kill rotifers while protecting the survival of algae as much as possible, and can effectively remove most of the pest pollution through the combined regulation of light induction and high-voltage discharge, so as to ensure that the growth of microalgae biomass and the accumulation of bioactive substances are less affected, and the application prospect is wide.

This application relates to the field of rotifer culture technology and discloses a method for regulating rotifer body size. This application successfully controls the body size of marine rotifers by regulating factors such as dissolved oxygen, temperature, salinity, and feed. Specifically, the conditions for large-scale rotifer culture include relatively low dissolved oxygen, temperature, and a larger feed, while the conditions for small-scale rotifer culture include relatively high dissolved oxygen, temperature, and a smaller feed. Certain cyanobacteria can also be used as supplementary feed. This application stably controls the environmental conditions for marine rotifer body size and selectively modifies the feed for the rotifers, achieving the goal of selectively regulating the body size of marine rotifers.

The present application relates to a kind of rotifer microflow water steady-state continuous expansion culture methods based on density-oviposition rate-ammonia nitrogen linkage control, suitable for the continuous supply of live bait in fry opening stage.The method is based on rotifer inoculation, microflow water change and a small amount of multiple feeding, and takes rotifer density, oviposition rate, total ammonia nitrogen and density variation coefficient as operation judgment index.When the total ammonia nitrogen in culture system increases, the oviposition rate decreases or the rotifer density fluctuates significantly in a short time, adjust the water change proportion, feeding frequency and harvesting proportion respectively.After the culture system reaches the preset steady-state condition, it is semi-continuous harvesting according to a certain proportion, and equal volume of new water and corresponding bait are added.The method can reduce the problems such as water quality deterioration, slow density recovery after harvesting and unstable continuous supply in fixed parameter culture.

The invention discloses an indoor cultivation method for increasing the survival rate of larvae and juveniles of horse mackerels, and relates to the technical field of aquaculture. Pretreated rotifers, shadberry fruit extracts and 2, 4 '-dihydroxychalcone are added in the early larva fish breeding stage and the later larva fish breeding stage, active ingredients such as gulcomannan, proline, chlorogenic acid and potentilla discolor extracts are added in the juvenile fish breeding stage, and meanwhile environmental factors such as illumination and sound wave stimulation are regulated and controlled, so that the juvenile fish breeding efficiency is improved. The survival rate of the horse mackerels and the capability of resisting infection of lactococcus gasseri and vibrio anguillarum can be effectively improved, the investment of the breeding cost is reduced, and the used baits are simple, easy to obtain and simple to prepare.

According to the technical scheme, the large yellow croaker large-scale breeding method suitable for mechanization is characterized in that seedling raising facilities are subjected to standardized transformation, for example, a water supply and drainage system and an inflation system of a seedling raising pond are optimized, special equipment such as an automatic bait casting machine and a dirt suction machine are integrated, and mechanical operation of key links such as bait casting and dirt suction is achieved. Meanwhile, by deploying a water quality on-line monitoring system and tracking key parameters such as dissolved oxygen and temperature in the whole process, operation standards such as water change, bait casting and dirt suction in each cultivation stage such as a rotifer stage, an artemia stage and a compound feed stage are quantified, and a digital management process is formed. According to the method, manpower dependence is effectively reduced, the standardization degree and efficiency of fry breeding operation are improved, and a solution is provided for quality improvement, efficiency improvement and transformation and upgrading of the large yellow croaker breeding industry.

The invention relates to a method and a system for high-density culture of rotifers. The method comprises the following steps: (1) providing a rotifer culture system, wherein the culture system comprises a conical culture barrel, a filter screen, a nano air stone, a water inlet pipe, a drain valve and an air delivery pipe; (2) disinfection treatment: carrying out disinfection treatment on a culture system and tools by using disinfection seawater; (3) inoculating and culturing rotifers: inoculating the domesticated rotifers into a culture system for culturing, feeding the rotifers by using concentrated chlorella with the particle size of 3-5 microns as bait, keeping the bait feeding period not more than 12 hours, keeping the water temperature of disinfected seawater used for culturing at 28-32 DEG C, the salinity at 13-17 per mill and the pH value at 7-9, and introducing pure oxygen through a microporous aeration device; sampling rotifers every day for microscopic examination, observing the water quality, measuring the density of the rotifers, and determining the dissolved oxygen, the water changing amount of the disinfected seawater and the bait feeding amount. According to the method, high-density rotifer culture can be achieved, cost is reduced, culture conditions are optimized, and stable supply of high-quality bait in aquaculture is guaranteed.

The invention relates to the technical field of rotifer culture, and discloses a culture method for regulating and controlling the body type of rotifers. According to the method, factors such as dissolved oxygen, temperature, salinity and bait for culturing the seawater rotifers are regulated and controlled, so that the purpose of regulating and controlling the body sizes of the seawater rotifers is successfully achieved; wherein the large-scale culture condition of the rotifer has relatively low dissolved oxygen and temperature and relatively large bait, and the small-scale culture condition of the rotifer has relatively high dissolved oxygen and temperature and relatively small bait, and meanwhile, some blue-green algae can be assisted. According to the application, the environmental condition of the body type size of the seawater rotifer is stably controlled, and the bait for feeding the seawater rotifer is selectively changed, so that the purpose of selectively regulating and controlling the body type of the seawater rotifer is achieved.

The invention relates to a rotifer high-density keep-alive transportation method. The method comprises the following steps: quantifying newly harvested rotifers, and mixing the quantified rotifers with fresh seawater in an original pool in proportion; transferring the mixed rotifers into a double-layer polyethylene plastic bag, sealing seawater ice with a sealing bag, and putting the seawater ice into the mixed live rotifers in proportion for cooling; long-acting oxygenation particles and an oxygenation synergist are put into the double-layer sealing bag for oxygenation, and then the double-layer polyethylene plastic bag is sealed after being filled with pure oxygen; the outer side of the box body is sleeved with a layer of polyethylene plastic bag, the box body is placed in a transportation box, the gap between the box body and the sealed plastic sealing bag is filled with the combination of the seawater ice bottle and the crushed seawater ice, finally, a cover is covered, and the transportation packaging box is sealed through an adhesive tape and then placed in a paper box to be sealed for transportation. The packaging sequence is a double-layer polyethylene plastic bag, a polyethylene plastic bag, a foam box and a paper box; after the rotifers are transported to the destination, the rotifers are put into a temporary rearing pond to be activated and then temporarily rearing, photosynthetic bacteria, EM bacteria and vitamin C are added into the temporary rearing pond, and the survival rate of the rotifers is increased.

The present invention provides a large-scale breeding method for saddle grouper, which relates to the technical field of grouper breeding. The method comprises digestion treatment of fertilized eggs and indoor and outdoor relay breeding. The digestion treatment of the fertilized eggs can facilitate the breaking of the membranes of the fish flowers; the rotifer eggs obtained by feeding the fry after strengthening during the indoor breeding process can provide the fry with more nutrients, which is conducive to the opening of the fry; the fry are cultured indoors when their physical conditions are weak in the early stages of flowering and metamorphosis, and then placed in outdoor ponds for breeding after they have a certain resistance, which can effectively improve the resistance of the fry. In addition, during outdoor breeding, the water body is continuously disinfected by using an electrolytic seawater chlorine dioxide generator to remove ammonia nitrogen and prevent the fry from being affected by pathogenic microorganisms. Under the above effects, the breeding survival rate can be improved.

The utility model discloses a rotifer ecological simulation breeding cabin, and relates to the technical field of fishery breeding devices. The top surface of the cabin body is hinged with a cabin cover; the inner bottom wall of the cabin body is provided with an electric heater; the top surface of the cabin cover is fixedly connected with a material storage barrel in a penetrating manner; the top surface of the material storage barrel is fixedly connected with a barrel cover; a stirring paddle is fixedly connected to the side wall of the lengthened shaft, a rotating ring is rotationally connected to the lower portion of the side wall of the discharging pipe, a gear ring is arranged on one side of the rotating ring, a protruding rod is fixedly connected to the side wall of the rotating ring, and a uniform distribution disc is fixedly connected to the end, away from the rotating ring, of the protruding rod; according to the rotifer feeding cabin, feed can be evenly distributed in the cabin body so as to avoid the situation that due to the fact that the feed is accumulated at one position, the local feeding amount is too large, the survival rate is improved, and it can be ensured that the water temperature is stabilized within a suitable range so that the survival of rotifers can be guaranteed.

The application discloses a single-cell blue algae separation and purification method based on rotifer enrichment and application, and the method comprises the following steps: placing a single-cell blue algae solution into a sterile BG11 conical flask; adding a rotifer solution with a known concentration into the sterile BG11 conical flask containing the single-cell blue algae solution to obtain a mixed solution of the rotifer and the single-cell blue algae; placing the sterile BG11 conical flask containing the mixed solution of the rotifer and the single-cell blue algae in a first culture environment for culture to obtain a blue algae and rotifer enrichment solution; transferring the blue algae and rotifer enrichment solution to a sterilized BG11 agar plate for plate coating treatment, and inverting the BG11 agar plate in a second culture environment for culture until a blue algae colony without bacteria is formed; and expanding the blue algae colony without bacteria to obtain single-cell blue algae without bacteria. The application can greatly shorten the time for traditional separation and culture of single-cell blue algae, and can prevent bacterial pollution from an external environment.

The application provides a method for improving the disease resistance of Litopenaeus vannamei "Guangtai No.1", wherein (1) the water temperature is 28.3-28.7 DEG C when the Litopenaeus vannamei "Guangtai No.1" juvenile enters a breeding pond, and then is increased to 29.8-30.2 DEG C, and then is decreased to 28.8-29.2 DEG C when the M2 of Tachycaris sp. reaches, and is decreased to 27.8-28.2 DEG C when the P2 of the juvenile shrimp reaches; (2) the density is 180-200 thousand / m 3 ; (3) the feed: the metamorphosis of the nauplius into the copepodid is started, and the feed of Chateoceros muelleri and Skeletonema costatum is started, and the total density is kept as 400-600 pieces / ml; the microcapsule feed is supplemented when the copepodid is at the second stage, and the feeding frequency is 5-6 times per day; the Skeletonema costatum is fed when the copepodid is at the third stage, and the density is 90-110 pieces / ml; the microcapsule feed, shrimp slices and rotifer are fed when the Tachycaris sp. is at the first and second stages; the nauplius of Artemia is fed twice and the micro-particle feed is fed four times when the Tachycaris sp. is at the third stage; the nauplius of Artemia is fed four times and the micro-particle feed is fed twice when the juvenile shrimp is at the first stage. The application effectively improves the disease resistance of the shrimp fry, and improves the survival rate and the qualified rate.

The invention relates to an indoor marsupenaeus japonicus offspring seed roughing culture method. The method comprises the following steps: (1) treating a roughing cement pond; the method comprises the following steps: (1) selecting an indoor cement pond for standard coarsening with the area of 10-100m < 3 > and the depth of about 1-2m, (2) feeding water, (3) fertilizing water: using fermented soybean meal and urea in an independent pond for rotifer cultivation (the inoculation density is 500,000 / mL), and putting the rotifer into a standard coarsening pond after the rotifer density reaches 2,000,000 / mL; (4) putting the young shrimps, (5) inoculating the copepods, (6) performing early-stage and daily management, and completing the thickness marking 20 days after the young shrimps are put until the sizes of the young shrimps reach 2.5-3cm. Through a symbiotic mode of microorganisms and shrimp seeds in the bait, growth of pathogenic organisms such as vibrio is inhibited, the survival rate of the shrimp seeds is increased, meanwhile, the utilization efficiency of the bait is optimized, the breeding cost is reduced, and therefore the overall economic benefit is improved.

The present application relates to the field of aquaculture technology, and particularly relates to a preparation method and application of seawater fry biological bait. The preparation method comprises the following steps: mixing Schizochytrium sp. powder, bile acid and sunflower phospholipid according to a mass ratio of 50:10-20:10-20 to obtain a nutrition enhancer; diluting the nutrition enhancer to a concentration of 40g / t-50g / t to obtain a diluent; adding 130-150 ordinary rotifers per milliliter of the diluent, and culturing for 12-18 hours to collect enhanced rotifers; adding 30-50 ordinary artemia per milliliter of the diluent, and culturing for 12-18 hours to collect enhanced artemia; and combining the enhanced rotifers and the enhanced artemia to obtain the fry biological bait. The fry biological bait prepared by the present application has uniform fry size and high survival rate during the bait conversion period, and has significant economic and ecological benefits.

The application discloses a method for synergistically strengthening Litopenaeus vannamei with probiotics and nutrients, and technical solution points of the method comprise the following steps: preparing water for breeding Litopenaeus vannamei, preparing a breeding pond, adding a probiotic group into the breeding pond, cultivating nauplii, adding chondrophycus into the breeding pond, cultivating copepodid larvae, feeding rotifer into the breeding pond, cultivating brine shrimp larvae, feeding shrimp slices into the breeding pond, feeding rotifer and brine shrimp nauplii together, cultivating postlarvae, feeding shrimp slices and brine shrimp nauplii into the breeding pond, and feeding rotifer, shrimp slices and brine shrimp nauplii after synergistic strengthening with probiotics and nutrients. The method has the advantages that the postlarvae feed is synergistically strengthened with probiotics and nutrients, the problems of large water spraying amount, easy strain inactivation and short effect period are avoided, the postlarvae is convenient to feed, the intestinal tract of the postlarvae is regulated, the digestion and absorption of nutritional components are promoted, the disease resistance of the postlarvae is improved, and the effect of promoting the production and cultivation of high-quality postlarvae is achieved.

The invention relates to a method for cryopreserving eggs, nauplii and young barnacles of barnacles. Furthermore, the present invention relates to the cryopreserved feed and the use of the preserved organisms as live feed organisms in marine aquaculture, typically in place of rotifers and artemia and other living feed organisms.

This invention relates to the field of aquaculture technology, specifically to a highly efficient and large-scale artificial breeding method for *Catfish bream*. The method includes a seedling cultivation step; the water in the seedling tank is obtained by treating fresh aquaculture water with fertilization, and the water in the seedling tank contains initial feed for *Catfish bream* fry; the initial feed includes rotifers and cladocerans. The fertilization method involves: after filling the seedling tank with fresh aquaculture water, sprinkling soybean milk, a dispersion of bacterial and algal nutrient paste, and a dispersion of algal polypeptides onto the water surface, and then adding rotifers to the water in the seedling tank. This technical solution can solve the technical problems of existing artificial breeding techniques for *Catfish bream*, which have many weaknesses and cannot meet the demands of modern aquaculture for efficient, stable, and high-quality seedling production. This technical solution has the advantages of improving breeding efficiency, ensuring seedling quality, adapting to large-scale production, reducing costs, and being environmentally friendly, making it suitable for efficient and large-scale artificial breeding of *Catfish bream*.