29results about How to "Good energy" patented technology

The invention discloses recombinant human copper / zinc superoxide dismutase as well as preparation and application thereof, which is characterized in that on the basis of an amino acid sequence of the human copper / zinc superoxide dismutase, a chloroplast Cu / Zn-SOD amino acid sequence of Withania somnifera and a Cu / Zn-SOD amino acid sequence of other biological species are referred to; the recombinant human copper / zinc superoxide dismutase (rmh Cu / Zn-SOD) with the amino acid sequence as shown in SEQ ID NO. 1 is obtained. Compared with wild type rmh Cu / Zn-SOD, the rmh Cu / Zn-SOD has the advantages that the water solubility and the enzyme specific activity are greatly improved, and the tolerance to an acid environment and the tolerance to trypsin are remarkably improved, so that the rmh Cu / Zn-SOD has higher commercial value and is particularly suitable for preparing oral SOD products.

The present invention relates to a fluoxazole amide composition that provides synergistic control of insect pests and mites under one-time application, and a method for preparing the same. More specifically, the present invention relates to a synergistic composition having a fluoxazole amide or an agrochemically acceptable salt thereof, at least one or more compounds selected from insecticides, at least one or more compounds selected from plant health additives, and a process for preparing said composition, and an agrochemically acceptable excipient. The invention also relates to a pesticidal composition which improves the health, yield, vigor, quality and tolerance to abiotic or biological stress of treated plants, prevents lodging of susceptible plants due to biological and abiotic factors such as heavy rain, wind, insects and disease damage, and also improves the yield, vigor, quality and tolerance to abiotic or biological stress. And provides residue control (i.e., longer duration control) and direct crop protection as well as effective control of insects and mites that are difficult to kill and have drug resistance.

The invention belongs to the technical field of microorganisms, and particularly relates to a bacterial strain for producing chitin deacetylase and application of the bacterial strain. The invention relates to a bacterial strain for producing chitin deacetylase, which is bacillus firmus, and is preserved in China General Microbiological Culture Collection Center (CGMCC) on October 30, 2024, and the preservation number is CGMCC NO.32397. The invention further discloses a method for producing the chitin deacetylase. According to the present invention, the chitin deacetylase is produced by using the chitin deacetylase producing strain screened from mangrove forest soil, the chitin deacetylase is applied to the biological preparation of chitosan, and compared with the currently researched chitin deacetylase, the chitin deacetylase produced by the strain has advantages of high activity, wide application range and industrial production potential.

The invention relates to the field of biology, and discloses a CHO-K1 cell strain capable of stably expressing IBV JS-96S1 protein, the cell strain can be well domesticated to obtain a suspended CHO-K1 cell strain, and the suspended CHO-K1 cell strain has the advantages of short domestication time and good cell state. Meanwhile, the invention also discloses a method for obtaining the suspended CHO-K1 cell strain by carrying out suspension domestication on the cell strain and application of the cell strain. In addition, the invention further discloses the IBV S1 protein and application.

The invention discloses a sparus latus kidney cell line and application thereof. The preservation number of the YSK of the kidney cell of the sparus latus is GDMCC No: 65080, and the preservation number of the YSK of the kidney cell of the sparus latus is GDMCC No: 65080. The sparus latus kidney cell line YSK is constructed through primary culture and subculture, the cellular morphology of the sparus latus kidney cell line is mainly fibroblast-like, the cells grow rapidly, stable passage exceeds 60 generations, and the activity is good after ultralow temperature storage. The sparus latus kidney cell line YSK cell line can be applied to marine fish virus separation, virus infection pathogenic mechanism and exogenous gene function research, and meanwhile, a good in-vitro cell amplification system is provided for preparation of sparus latus dephosphorization virus SDDV vaccines.

The present invention provides a diluent for improving the quality and fertilization ability of frozen bovine semen, including chlorogenic acid and a calcium chelator. Compared with the prior art, the present invention adds antioxidant chlorogenic acid or caffeic acid and a calcium chelator to the semen cryopreservation diluent, effectively improves the vitality, plasma membrane integrity, acrosome integrity and fertilization ability of frozen-thawed semen, significantly improves the antioxidant performance, reduces the ROS level, inhibits premature capacitation at low temperature, and significantly improves the cryopreservation effect of bovine sperm. It is suitable for long-term cryopreservation of bovine semen in liquid nitrogen and artificial insemination. The diluent preparation method is simple, the cryopreservation operation method is simple, and it is easy to promote, and significantly improves the quality and fertilization ability of bovine semen after freezing and thawing.

The present invention provides an energy transmitting fiber and preparation method thereof, and a fiber laser. The energy transmitting fiber includes a core layer and a cladding layer, where from a center of the energy transmitting fiber to an outer wall of the energy transmitting fiber, the core layer includes an inner core layer, an inner sinking layer and an outer core layer that are cladded sequentially, and the cladding layer is cladded on an outer wall of the outer core layer; along a direction perpendicular to an extension direction of the energy transmitting fiber, a cross-section of the inner core layer is in a circular shape, a cross-section of the inner sinking layer is in a ring shape, a cross-section of an inter wall of the outer core layer is in a circular ring shape, an outer wall of a cross-section of the outer core layer is in a rectangular ring shape, the inner core layer and the inner sinking layer are concentrically disposed, and the outer core layer is centrally symmetric along a center of the inner core layer; at least two of the inner core layer, the inner sinking layer and the outer core layer have different refractive indices. A light beam outputted by the energy transmitting fiber of the present invention has good energy uniformity, and when using the fiber laser including the energy transmitting fiber to cut a plate, it is not easy to cause damage to the plate.

This invention discloses a chitosan-based hydrogel contraceptive material, its preparation method, and its applications, relating to the field of contraceptive materials technology. The material comprises: perfluoroalkyl chain modified chitosan: 1-5 wt%; sodium β-glycerophosphate: 3-10 wt%; polyacrylic acid: 0.5-3 wt%; spermicide: 0.1-5 wt%; silver nanoparticles: 0.01-0.1 wt%; chitosan oligosaccharide: 0.1-1 wt%; lactic acid bacteria metabolites: 0.05-0.5 wt%; and lactic acid aqueous solution: balance to 100%. The thermosensitive gelation mechanism of this invention utilizes perfluoroalkyl chain modified chitosan, which is a material with a lowest critical solution temperature (LCST) of 37°C. The complex interaction between the spermicide and the gel matrix consists of intermolecular van der Waals forces and hydrogen bonds.

The invention discloses a pharmaceutical composition for reinforcing the kidney and producing sperms as well as a preparation and application of the pharmaceutical composition for reinforcing the kidney and producing sperms. The traditional Chinese medicine composition is prepared from prepared rehmannia root, deer-horn glue, salted flatstem milkvetch seed, radix scrophulariae, radix rehmanniae recen, codonopsis pilosula, astragalus membranaceus, roasted astragalus membranaceus, fructus lycii, salted semen cuscutae, raspberry, wine-processed teasel root, salted eucommia ulmoides, roasted rhizoma polygonati, roasted rhizoma atractylodis macrocephalae, pericarpium citri reticulatae, poria cocos, longan aril, bran-fried Chinese yam, Chinese date, salted semen plantaginis, leonurus, salvia miltiorrhiza, centipede, ligusticum wallichii, caulis spatholobi, radix bupleuri, vinegar-processed schisandra chinensis and calcined dragon bone. The pharmaceutical composition for reinforcing kidney and producing sperm is added with pharmaceutically acceptable auxiliary materials to prepare a preparation. The pharmaceutical composition for reinforcing kidney and producing sperm and the preparation thereof can be used for preparing medicines for invigorating spleen and replenishing qi, promoting qi to activate blood, and tonifying kidney and producing sperm.

The present invention belongs to the field of cell culture technology, and specifically relates to a method for isolating and primary culturing adult Muscovy duck cardiomyocytes. In the culturing method of the present invention, the steps for isolating and purifying adult Muscovy duck cardiomyocytes are simple, the cells adhere quickly, the survival rate is high, the activity is good, and the cell culture system has good stability. The method is a relatively ideal method for culturing primary Muscovy duck cardiomyocytes, which can basically meet the requirements of various research fields of medicine, biology, pharmacy and life sciences for experimental research related to Muscovy duck cardiomyocytes, and is suitable for promotion and application in general laboratories.

The invention discloses a rainbow trout compound feed using chicken gizzard lining dregs to replace fish meal, comprising the following raw materials by weight: 59.5-62.3 parts of protein source raw materials, 14.3-15.5 parts of fat source raw materials, 14 parts of sugar source raw materials, 1 part of complex vitamins, 1 part of complex minerals, 1.60-3.47 parts of functional amino acids, 0.5 parts of antioxidants, 1 part of calcium dihydrogen phosphate, 0.5 parts of choline chloride, 1 part of sodium alginate, 0.05 parts of astaxanthin, and 0.88-4.35 parts of carboxymethyl cellulose; the protein source raw materials are composed of the following raw materials by weight: 25-45 parts of fish meal, 5-25 parts of chicken gizzard lining dregs, 3.5-6.3 parts of soy protein isolate, and 6 parts of soybean meal. The feed uses chicken gizzard lining dregs as a protein source raw material and reduces the use of fish meal, solves the problems of insufficient supply of fish meal and high feed production cost, realizes the sustainable development of the rainbow trout breeding industry chain, and has great economic and social benefits.

This invention discloses a herbal compound-based hair growth and anti-hair loss conditioning cream and its low-temperature preparation process, relating to the fields of cosmetics and scalp care technology. Specifically, it is a herbal compound-based hair growth and anti-hair loss conditioning cream and its low-temperature preparation process. The conditioning cream contains, by weight, herbal active ingredients such as ginseng, arborvitae leaf, duckweed, soapberry, angelica, chuanxiong, rosemary, ginger, asarum, and mulberry bark, as well as functional and matrix excipients such as sodium hyaluronate, cocoyl glucoside, carbomer 940, phenoxyethanol, and ethylhexylglycerin. Its preparation process employs low-temperature treatment throughout, including low-temperature extraction at room temperature to 50℃ and ultrasonic extraction at 200-800W, as well as low-temperature blending with low-speed stirring at 25-40℃, effectively preserving the active herbal ingredients. This product can improve scalp microcirculation, repair hair follicles, and regulate sebum secretion, suitable for various types of hair loss, while also relieving scalp itching and repairing damaged hair.

The invention discloses a cell co-culture device, which relates to the field of cell culture. The device is composed of a dish body and a dish cover. The dish body is a hollow structure with an upper opening. The dish cover is provided at the opening of the dish body. The dish body is provided with an isolation ring. The isolation ring divides the interior of the dish body into region I and region II. The isolation ring contains a crawling sheet. The device can be used to realize direct contact co-culture of bovine muscle cells and fat cells, and single cells (muscle cells or fat cells) on the crawling sheet can be collected for subsequent transcriptome and proteome analysis and processing to study the effects on muscle cells or fat cells in a co-culture environment. Therefore, it is beneficial to further explore the interaction between muscle cells and fat cells. It has been confirmed through multiple observations that the muscle cells and fat cells cultured in this way have high survival efficiency, good vitality, obvious cell characteristics, and are consistent with the morphology of muscle cells and fat cells cultured alone. It is a good in vitro research model with certain promotion and research value.

According to the invention, a proper amount of yeast extract powder, soy peptone, fructose, anhydrous magnesium sulfate, L-cysteine hydrochloride monohydrate, sodium carbonate and monopotassium phosphate are used to form the non-animal-origin liquid culture medium, and in the process of culturing Cristinia parvata by using the non-animal-origin liquid culture medium, the Cristinia parvata proliferates quickly. And the kristinia parvata cultured by adopting the non-animal-origin culture medium is good in growth state, good in safety, easy to operate and control and long in culture time, and meets the industrial requirements of medicinal values. In addition, when the non-animal-origin culture medium is used for culturing the kristinia parvata, the viable count is high, the strain activity is high, the passage stability is good, and the kristinia parvata can be subjected to large-scale industrial production.

The invention relates to the technical field of medicines, and discloses a traditional Chinese medicine composition for treating oligoasthenospermia and a preparation method and application thereof. The traditional Chinese medicine composition is prepared from the following raw material medicines in parts by weight: 10-15 parts of fig, 10-15 parts of eyebrow bean, 10-15 parts of Chinese yam, 5-10 parts of black peanut, 5-10 parts of sea-buckthorn, 5-10 parts of gordon euryale seed, 5-10 parts of radix cynanchi bungei, 5-10 parts of pomegranate, 5-10 parts of hawthorn, 5-10 parts of cedrela sinensis, 5-10 parts of maca, 5-10 parts of fingered citron and 5-10 parts of date palm. , 5-10 parts by weight of rhizoma polygonati, 5-10 parts by weight of Chinese wolfberry, and 5-10 parts by weight of whole litchi. The medicine prepared from the traditional Chinese medicine composition disclosed by the invention has the effects of tonifying kidney and spleen, promoting diuresis and activating blood, and producing and strengthening sperms, is expected to become a medicine for clinically treating oligospermia, weakness and abnormal spermatospermia, is easy to store and transport, and is beneficial to popularization and application.

The invention discloses an oral soluble film composition containing vardenafil and application of the oral soluble film composition. The oral soluble film composition comprises the following components in percentage by weight: 20.5%-25% of an active substance, 28.3%-32% of microcrystalline cellulose, 2.6%-3.3% of vitamin E, 1.5%-2.1% of a separant, 0.8%-1.1% of a lubricant, 35.6%-41.5% of a film forming material, 1.2%-1.8% of a plasticizer, 0.08%-0.12% of a pH regulator and 2.7%-3.5% of a flavoring agent, the active substance is vardenafil or vardenafil hydrochloride, the concentration of the active substance is 35-45 mg / ml, and the oral soluble film composition is applied to improving the erectile function, prolonging the sexual life time, improving the microenvironment of local spermatogenesis and improving the sperm vitality. The pH regulator is matched, so that the dissolution speed of the medicine can be increased; 40-60 hydroxypropyl methyl cellulose and polyvinyl alcohol are blended to form a film material, so that the film coating effect can be ensured, and the properties of the film can be improved; vitamin E is added in the formula, so that the microenvironment of local spermatogenesis can be improved, the sperm motility is improved, and the medicinal value of the composition is improved.

The present invention belongs to the field of cell biology, and specifically relates to a cell bionic drying model and its application in the evaluation of moisturizing efficacy or dryness damage repair efficacy. The present invention establishes a method for evaluating the moisturizing efficacy or dryness damage repair of cells under culture conditions that only change the humidity, and a method for detecting the cell viability after drying. The drying conditions are relatively mild, simulating the growth environment of cells in the human body, the drying modeling time is appropriate, the cell death rate is slow, the influence of operation errors is smaller, and the degree of recovery of cell viability after drying can be more intuitively reflected, which can be applied to the high-throughput screening of moisturizing active substances in cosmetics.

The invention discloses an N-methyl nitrosamide compound as well as a pharmaceutical composition and application thereof. The compound (formula I) is a hypoxia-activated NO donor drug, has the characteristic of effectively releasing NO under a high-selectivity hypoxia condition, so that the activity of myocardial cells is remarkably improved, the in-vivo and in-vitro activity is excellent, and the compound can be used for preventing or treating myocardial hypoxia injury. Meanwhile, the metabolic stability of the compound is improved by an ether bond connection structure, so that the compound has in-vivo metabolic properties beneficial to patent medicines.

The application provides a method for cryopreservation and ultra-cryopreservation of the sperm of feed domesticated mandarin fish, and first provides a cryopreservation diluent for the sperm of the mandarin fish, which comprises Hanks solution, sodium chloride, soybean lecithin, vitamin E, curcumin, melatonin and SOD; an anti-freezing protective agent is added in the cryopreservation diluent to obtain an ultra-cryopreservation anti-freezing solution for the sperm of the mandarin fish; the sperm of the mandarin fish is mixed with the cryopreservation diluent to realize cryopreservation; and the sperm of the mandarin fish is mixed with the ultra-cryopreservation anti-freezing solution to realize ultra-cryopreservation. The cryopreservation diluent for the sperm of the mandarin fish can realize cryopreservation of the sperm of the mandarin fish, and the sperm can be ultra-cryopreserved after the addition of the anti-freezing protective agent, so that the persistence of the preservation time of the sperm of the mandarin fish is ensured, the protection effect on the sperm is good, the activation rate of the sperm after thawing is high, and the sperm has good vitality.

This invention discloses a macrophage digestion solution and a method specifically for macrophage digestion, comprising the following components: 3-6 parts ethylenediaminetetraacetic acid (EDTA), 4-8 parts ethylene glycol bis(2-aminoethyl ether)tetraacetic acid (EDTA), 8-12 parts fetal bovine serum (FBS), and 90-100 parts phosphate buffer. The method specifically for macrophage digestion includes the following steps: preparing a stock solution of digestion solution, filtering it, and storing it at low temperature for 6 months to obtain the macrophage digestion solution; adding the macrophage digestion solution to a macrophage culture flask; shaking the macrophage culture flask containing the digestion solution to ensure even coverage; adding DMEM culture medium to the settled macrophage culture flask to neutralize the digestion solution, and transferring it to a centrifuge tube; centrifuging the solution in the centrifuge tube at room temperature for 3 minutes; and adding DMEM culture medium to the centrifuge tube after centrifugation to complete the macrophage digestion. This invention achieves high macrophage digestibility and low differentiation rate.

Discussed is an energy storage system may that can include: a battery system including one or more batteries; a power conditioning device (PCS) configured to control power of the battery system; and a power management controller (PMS) configured to collect state information on one or more of the grid, the PV system, the EV charging system, the battery system, and the PCS, and to control powers of the PV system, the EV charging system, and the battery system based on the collected state information.

The invention belongs to the field of annona squamosa pollen detection, and particularly relates to a method for rapidly detecting the vitality of annona squamosa pollen. Compared with traditional FDA-PI double staining, the mixed solution for pollen viability detection has longer fluorescence lifetime, the pollen maintains the form without bursting within a certain time, the in-vitro germination process can be continuously observed, the verification of the detection result of pollen viability is facilitated, and the accuracy of the detection result is improved. The mixed solution can count the number of pollen cell nucleuses, helps to determine the pollen development stage, can mutually verify the pollen activity detection result, is convenient to comprehensively judge the pollen activity, is higher in dyeing speed, does not have processes such as centrifugation, and is simpler and more convenient to operate.

The present specification relates to a composition capable of ameliorating skin viability due to reduced stress. The composition according to the present disclosure contains a thymol ester compound as an active ingredient, thereby being capable of promoting ATP production through mitochondrial activity, and further repairing skin cell viability reduced due to stress.

The application discloses a kind of citric acid-microbial inoculant coproduction process methods.The process method described in the application is as follows: (1) citric acid fermentation liquor is subjected to plate and frame filtration treatment, and mycelium and citric acid feed liquid are separated, the citric acid feed liquid is neutralized, and calcium citrate and raffinate are obtained after filtration, calcium citrate enters subsequent extraction process, and citric acid is prepared; and raffinate enters evaporator for concentration, and concentrated liquid and condensate are obtained; (2) the condensate is used as citric acid production raw material mixing water, and the concentrated liquid is used for culturing microorganisms after pretreatment, and microbial inoculant is obtained; (3) then the microbial inoculant is centrifuged, and concentrated microbial inoculant and tail liquid are obtained, the concentrated microbial inoculant is added with protective agent and carrier, and microbial inoculant is obtained by spray drying.The method described in the application uses citric acid production raffinate as microbial culture nutrient source, realizes high-value and resource utilization of nutrient substances in raffinate, significantly reduces water consumption, and reduces sewage discharge.

The present invention discloses a class of NO donor compounds and their preparation methods, pharmaceutical compositions and applications. The compound structure is shown in Formula I. The NO donor compounds and their pharmaceutical compositions can be prepared as drugs for treating myocardial hypoxia injury diseases as hypoxia-activated NO donors. The prepared drugs can exert their efficacy at both the cellular level and the animal level, and the synthesis method of the compounds is simple and easy to operate.

The invention discloses a simple field preparation method of dictyophora indusiata cultivated species in the technical field of dictyophora indusiata cultivation. The simple field preparation method comprises the following steps: step 1, preparing a fermentation material; step 2, building a heap and fermenting; step 3, turning the pile; after pile building is completed, the material pile is turned through a pile turning device every 7-10 days, and meanwhile the pile turning device properly sprays water to the material pile according to the humidity degree of the compost; 4, field preparation; 5, soil preparation and furrow bed forming; 6, sowing and earthing in a field; laying a culture material on the furrow bed, inoculating a strain on the surface layer of the culture material, covering a layer of culture material, covering soil on the furrow surface, and covering straw after the soil covering is completed; 7, field cultivation and irregular detection are carried out. According to the method, the characteristic that the dictyophora indusiata is good in field hybrid resistance is utilized, the dictyophora indusiata strains are simply and rapidly bred in the field after rice is harvested, the cost is reduced, the quality of dictyophora indusiata finished products is improved, and meanwhile the dictyophora indusiata cultivation period is shortened.

The invention belongs to the field of biological medicine and relates to a preparation method of bone marrow mesenchymal stem cell exosomes and an application of the bone marrow mesenchymal stem cell exosomes in repairing an articular cartilage defect. The bone marrow mesenchymal stem cell exosomes are generated by stimulating mesenchymal stem cells with a culture medium, and extracted from a culture solution after passage; the bone marrow mesenchymal stem cells are derived from bone marrow of ilium or femur. An extracellular vesicle prepared from bone marrow mesenchymal stem cells has typical characteristics of an exosome; the prepared exosomes can improve cartilage cell vitality and promote the activity of cartilage cell proliferation and migration; in a rabbit articular cartilage defect model, the prepared exosomes can significantly promote cartilage repair.

The present application belongs to the field of medical technology, and discloses compounds in Apricot-scented Rabbit Ear Wind and its preparation method and application. The compound is compound 1 or compound 2. Apricot-scented Rabbit Ear Wind is extracted with alcohol, and petroleum ether, dichloromethane, ethyl acetate, and water are added for extraction respectively. The water-extracted part is first separated by resin, and then separated by silica gel column chromatography. The fractions from different parts are separated by preparative high-performance liquid chromatography to obtain the target compound 1 or compound 2. The preparation method of the compound of the present invention is simple to operate, and the compound in the Apricot-scented Rabbit Ear Wind can be separated. Experiments show that the compound of the present invention can effectively improve the death of AC16 human cardiomyocytes induced by doxorubicin and the disorder of GSH and inflammatory factor IL6. The present invention provides that the compound has a good protective effect on myocardial damage, has little toxic side effects, and can be used for the preparation of anti-myocarditis drugs.

This application provides a positive electrode plate for lithium-ion battery and a lithium-ion battery containing the same. The positive electrode plate includes a current collector, and a capacity layer and a power layer that are specially designed, able to effectively eliminate, in an economical and simple manner, the adverse impact on the battery performance caused by a product resulting from a pre-lithiation material releasing lithium during charge-discharge cycles, thereby significantly improving the first-cycle coulombic efficiency, cycling performance, and service life of batteries.