Described herein are variations of an
analyte monitoring system, including an
analyte monitoring device. For example, an
analyte monitoring device may include a
skin-penetrating microneedle array for use in measuring analyte, such as in a continuous manner. The microneedle array may include, for example, at least one microneedle comprising a
working electrode comprising an analyte-sensing
aptamer that selectively and reversibly binds to analyte. The
working electrode comprises at least one
thiol-based
passivation molecule that provides protection of the
working electrode from, by way of example, foulants and interferents. For example, a sensor may be configured to generate a
signal that is indicative of a concentration of an analyte in a fluid, the sensor comprising a working
electrode comprising an
electrode material, a biorecognition layer disposed at least partially on the
electrode material and comprising a biorecognition element that selectively and reversibly binds to an analyte, and an at least partially desiccated hydrogel disposed on the biorecognition layer. For example, a working electrode may be configured to generate a
signal indicative of a concentration of an analyte in a fluid, the working electrode comprising an
electrode material, a biorecognition element disposed on the
electrode material that selectively binds to the analyte, wherein the biorecognition element is functionalized with a
redox-active molecule and is configured to change conformation upon binding the analyte to move the
redox-active molecule, closer to, or further from, the
electrode material, and a
passivation element comprising a zwitterionic
peptide disposed on the electrode material.