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Up/down conversion dual-mode fluorescent nanomaterial for Nd<3+> sensitization and synthesis method thereof

A fluorescent nanomaterial and fluorescence technology, applied in the field of up/down conversion dual-mode fluorescent nanomaterial and its synthesis, can solve the problems of reducing the excitation efficiency of excitation light, burning, and damage to the object under inspection.

Inactive Publication Date: 2014-06-18
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This absorption will not only reduce the excitation efficiency of the excitation light, the most important thing is that the excitation light absorbed by the water will be converted into a large amount of heat, which will further cause damage or burns to the tested object

Method used

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  • Up/down conversion dual-mode fluorescent nanomaterial for Nd&lt;3+&gt; sensitization and synthesis method thereof
  • Up/down conversion dual-mode fluorescent nanomaterial for Nd&lt;3+&gt; sensitization and synthesis method thereof
  • Up/down conversion dual-mode fluorescent nanomaterial for Nd&lt;3+&gt; sensitization and synthesis method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: NaGdF 4 :NdNaYF 4 NaGdF 4 :Nd,Yb,ErNaYF 4 Down-converting nuclear isolation shell 1 Up-converting shell 2 Protective shell 3 Excitation of Nd at 800 nm 3+ Preparation of sensitized up / down-converting bimodal fluorescent nanomaterials. Specific steps are as follows:

[0024] (1) Preparation of the shell precursor.

[0025] Preparation of the precursor of Y-OA (0.1 M): Take a 50 mL three-neck round bottom flask as the reaction vessel, add 2.5 mmol YCl in sequence 3 , 10 mL oleic acid (OA), 15 mL octadecene (ODE). Heating to 140 °C for 1 h under vacuum and magnetic stirring conditions finally yielded a clear and transparent precursor of Y-OA (0.1 M).

[0026] Preparation of Gd-OA (0.10 M), Nd-OA (0.10 M), Yb-OA (0.10 M), Er-OA (0.10 M), and Tm-OA (0.10 M) precursors: Gd-OA, Nd The preparation of -OA, Yb-OA, Er-OA, Tm-OA is similar to the preparation of the above-mentioned Y-OA precursor, and the above-mentioned YCl 3 Replaced by GdCl respectively 3 ,...

Embodiment 2

[0037] Example 2: NaYF 4 :Nd,Yb,TmNaYF 4 NaYF 4 :NdNaYF 4 Up-conversion nuclear isolation shell 1 Down-conversion shell 2 Protective shell 3 800 nm excitation Nd 3+ Preparation of sensitized up / down-converting bimodal fluorescent nanomaterials. Specific steps are as follows:

[0038] (1) Preparation of the shell precursor.

[0039] Preparation of the precursor of Y-OA (0.1 M): Take a 50 mL three-neck round bottom flask as the reaction vessel, add 2.5 mmol YCl in sequence 3 , 10 mL oleic acid (OA), 15 mL octadecene (ODE). Heating to 140 °C for 1 h under vacuum and magnetic stirring conditions finally yielded a clear and transparent precursor of Y-OA (0.1 M).

[0040] Preparation of Gd-OA (0.10 M), Nd-OA (0.10 M), Yb-OA (0.10 M), Er-OA (0.10 M), and Tm-OA (0.10 M) precursors: Gd-OA, Nd The preparation of -OA, Yb-OA, Er-OA, Tm-OA is similar to the preparation of the above-mentioned Y-OA precursor, and the above-mentioned YCl 3 Replaced by GdCl respectively 3 , NdCl 3...

Embodiment 3

[0051] Example 3: NaGdF prepared in example 1 4 :NdNaYF 4 NaGdF 4 :Nd,Yb,ErNaGdF 4 Down-converting core isolation layer, up-converting layer, passivation layer, core-shell structure nanocrystals have a hydrophobic surface, and modification of amphiphile molecules can make the obtained nanocrystals water-soluble, so that they have better biocompatibility . The resulting water-soluble nanocrystals are incubated with cells, and the nanoparticles enter the cells through endocytosis. Using the nanocrystals with both green and red visible light up-conversion fluorescence and near-infrared down-conversion fluorescence, we can perform multi-mode imaging of cells . In addition, by injecting the obtained water-soluble upconversion nanocrystals into mice through the tail vein, we can use the near-infrared downconversion fluorescence of nanoparticles for in vivo imaging. Since the excitation light source of the down-conversion near-infrared fluorescence of the nanoparticle is also...

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Abstract

The invention belongs to the technical field of nanometer biological materials, and particularly relates to an 800nm excitation-based up / down conversion dual-mode fluorescent nanomaterial for Nd<3+> sensitization and a synthesis method thereof. The synthetic up / down conversion dual-mode fluorescent nanomaterial for Nd<3+>sensitization designed by the invention has a multi-layer core-shell structure, and comprises a down conversion luminous layer, an up conversion luminous layer, an isolation layer and a passivation layer. Different layers of the material synergistically play respective different roles, and meanwhile, the dual-mode fluorescent probe with up conversion fluorescence and down conversion fluorescence is finally achieved under an 800nm of excitation light with a low-heat effect. The up conversion excitation light is expanded to about 800nm from 980nm by Nd<3+>, Yb<3+> and Er<3+>-doped NaGdF4:Nd, Yb, Er up conversion layer due to introduction of Nd<3+>, and the fluorescent process from a near infrared light to a visible light is achieved. The process meets the requirements of an in-vitro fluorescent probe. In addition, the typical down conversion fluorescence from the near infrared light to a far infrared light is also achieved in one nanoparticle due to the synergistic effect of the NaGdF4:Nd core.

Description

technical field [0001] The invention belongs to the technical field of nano-biological materials, in particular to a kind of Nd 3+ Sensitized up / down-converting dual-mode fluorescent nanomaterials and their synthesis methods. Background technique [0002] With the rapid development of nanotechnology, a variety of high-quality rare earth-doped upconversion fluorescent nanoparticles have been synthesized and successfully applied in various fields (especially in the field of life sciences). Compared with traditional biomarkers (such as organic dyes and quantum dots, etc.), upconversion fluorescent nanomaterials have many advantages, such as high chemical stability, low toxicity, and high signal-to-noise ratio. Scientific researchers have proved that the "near-infrared window" is particularly important for both in vitro and in vivo applications. Because near-infrared light has the following advantages: it can effectively reduce biological background fluorescence, relatively sm...

Claims

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Application Information

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IPC IPC(8): C09K11/85C09K11/02
Inventor 张凡李晓民赵东元
Owner FUDAN UNIV
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