5694 results about "Biocompatibility" patented technology

The invention discloses a polypropylene composite material based on rare earth element modification and a preparation method and application thereof, and belongs to the technical field of medical instruments, and the polypropylene composite material comprises the following raw materials in parts by weight: 100 parts of medical grade PP, 3-8 parts of a rare earth organic complex, 5-10 parts of nano-hydroxyapatite, 2-5 parts of a maleic anhydride grafted PP compatilizer and 0.3-0.5 part of an antioxidant, the invention also provides specific preparation steps and application thereof. According to the invention, oxide + salt bimorphological rare earth and an organic ligand are complexed to form a rare earth organic complex, so that the problem of poor dispersity of inorganic rare earth is solved, and meanwhile, the material is endowed with antibacterial and antistatic functions; through a melt blending-in-situ fiber forming process, the rare earth complex forms gradient distribution in a polypropylene matrix, the surface layer is enriched with antibacterial components, and the inner layer is enhanced in mechanical property, so that a low-cost solution with high strength, antibacterial property and biocompatibility is provided for high-end medical instruments, and the market blank is filled.

The invention belongs to the technical field of biomedical engineering, and particularly relates to a biological rotator cuff patch with a double-gradient structure and a preparation method of the biological rotator cuff patch. The preparation method comprises the following steps: carrying out electrostatic spinning on a spinning solution, controlling the rotating speed of a receiving roller to increase at a constant speed in the electrostatic spinning process, and obtaining a nanofiber membrane after electrostatic spinning; l-polylactic acid and D-polylactic acid are simultaneously dissolved in the spinning solution; the minimum value of the rotating speed of the receiving roller is 100-500 rpm, and the maximum value of the rotating speed of the receiving roller is 3000-4000 rpm. The finally prepared biological rotator cuff patch with the double-gradient structure presents an orientation degree gradient and a piezoelectric effect gradient at the same time on the thickness scale, and has mechanical supporting capacity and biocompatibility, and bionic healing promotion of the patch with the gradient structure is achieved.

The present invention belongs to the technical field of biomedical materials. Disclosed are a methacrylate, a succinate-diesterified starch, a preparation method therefor and the use thereof in an in-situ filler for corneal defects. The methacrylate, succinate-diesterified starch is prepared by means of performing graft carboxylation on starch and then grafting a double bond. The in-situ filler for corneal defects consists of the methacrylate succinate-diesterified starch (StacMA), gelatin methacryloyl (GelMA), a photoinitiator and a solvent. The corneal filler has a good biocompatibility, an excellent photoresponsive adhesion property and excellent mechanical properties, and can be used for promoting the regeneration of the natural structure of a damaged cornea and the reconstruction of visual function.

The invention relates to an implantable biological electrode and a manufacturing method of an implantable glucose monitoring probe. The manufacturing method of the implantable biological electrode comprises the following steps: S1.1, providing a flexible substrate; s1.2, silk-screening silver ink on the first surface of the flexible substrate to form a non-implanted conductive region of the first functional electrode; s1.3, silk-screening carbon ink on the first surface of the flexible substrate to form an implanted conductive region of the first functional electrode, and forming a series structure by the non-implanted conductive region and the implanted conductive region to jointly form a conductive region of the first functional electrode; and S1.4, an insulating layer is screen-printed on the first surface of the flexible substrate, the insulating layer covers the conductive region of the first functional electrode, and the working region is exposed. According to the functional electrode, the characteristic of good biocompatibility of the carbon circuit layer and the characteristics of small resistance and high signal transmission efficiency of the silver circuit layer are fully utilized, the circuit impedance of the functional electrode is effectively reduced, the signal transmission efficiency is improved, and the biocompatibility and long-term stability of an implantation area are ensured.

The invention provides a 3D biological printing osteochondral organ based on dynamic mechanical regulation and control, and a construction method and application thereof. Compared with the prior art, the invention innovatively provides a biphasic scaffold optimization modification strategy: firstly, an osteochondral hydrogel scaffold with an accurate layered structure is prepared by adopting a 3D biological printing technology, then a cell mechanical transduction signal channel is activated by a dynamic pressure stimulation system, and cartilage / bone biphasic differentiation of stem cells is directionally regulated and controlled; and by constructing a bionic mechanical microenvironment, the obtained osteochondral organ not only keeps excellent biocompatibility, but also has gradient mechanical properties and a clear spatial layered structure (cartilage layer-bone layer), the directional cartilage formation / osteogenic differentiation efficiency of the osteochondral organ is remarkably improved, and the osteochondral organ has a good application prospect. The problems of spatial heterogeneity construction and accurate regulation and control of cell fate in osteochondral tissue engineering are effectively solved, and the method has important application value in the fields of osteochondral defect repair treatment and mechanical stimulation mechanism research.

The invention relates to a dynamic-covalent hybrid cross-linked hydrogel material as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving a component A-phenylboronic acid modified polymer derivative in a biocompatible medium to obtain a solution A; dissolving a component B, namely a macromolecular derivative containing a vicinal diol group, into a biocompatible medium to obtain a solution B; dissolving a component C-double bond group modified polymer derivative in a biocompatible medium to obtain a solution C; and mixing the solution A, the solution B and the solution C with certain concentrations for a certain time, and performing illumination under a photoinitiator for a certain time to obtain the dynamic-covalent hybrid cross-linked hydrogel material. Compared with single dynamic bond crosslinking or covalent bond crosslinking, the prepared hydrogel material has machinability and mechanical stability; the hydrogel material has plasticity, shear thinning, self-repairing property and the like in the early stage of crosslinking; enough mechanical strength and long-term stability are realized in the later stage of crosslinking.

The invention discloses a porous titanium-based implant with a photo-thermal surface and a preparation method and application of the porous titanium-based implant, and belongs to the technical field of biomedical materials. The preparation method comprises the steps that a porous titanium substrate is prepared through 3D printing, then flowing acid etching treatment and anodic oxidation treatment are conducted in sequence, then the porous titanium substrate is placed in photo-thermal coating dispersion liquid for reaction, cleaning and drying are conducted, and the porous titanium-based implant with the photo-thermal surface is obtained. The photo-thermal coating dispersion liquid is obtained by dispersing MXene-ZnO composite particles in a dopamine hydrochloride solution. The porous titanium-based implant provided by the invention can be rapidly heated to a proper temperature under the irradiation of near-infrared light, and has good antibacterial, anti-tumor and osteogenesis promoting properties and biocompatibility.

The invention belongs to the field of synthetic biology, and particularly relates to collagen fusion polypeptide as well as application and a preparation method thereof. The collagen fusion polypeptide provided by the invention comprises: (a) a first polypeptide of recombinant III-type collagen; and (b) a second polypeptide comprising a trimerized folding domain. The collagen fusion polypeptide provided by the invention has the advantages of low immunogenicity, good biocompatibility, high biological activity and the like, can significantly promote proliferation and migration of human skin fibroblasts, and has stronger ability of promoting regeneration of endogenous collagen. The collagen fusion polypeptide has a triple helix structure similar to natural collagen, and can be self-assembled to form collagenous fibers which are good in morphology and similar to natural collagen. The method can be widely applied to the fields of skin repair dressings, biological materials, artificial implants, medical instruments, cosmetics, health care products, food and the like.

The invention discloses an edentulous jaw dental implant positioning method combined with depth variation reasoning. The method comprises the following steps: S1, generating optimized medical image data; s2, generating a jaw bone three-dimensional reconstruction model of the edentulous jaw patient; s3, generating anatomical feature data; s4, generating a preliminary implant positioning scheme; s5, generating a local bone reconstruction simulation result; s6, performing dynamic interaction on a local bone reconstruction simulation result and the preliminary implant positioning scheme, optimizing the preliminary implant positioning scheme through a depth variation inference model, and generating an implant optimized positioning scheme including mechanical stability and biomechanical performance evaluation; and S7, based on the implant optimization positioning scheme, generating implant guide plate design data and surgical navigation data in combination with the jaw bone three-dimensional reconstruction model of the patient. According to the method, the final implant positioning point obtains a globally optimal solution in the aspects of long-term stability and biological adaptability.

The invention discloses a wearable medical monitoring equipment electrode which is composed of a layered structure and comprises a transparent flexible substrate layer which is made of a biocompatible flexible polymer material and is used for providing flexible support and isolating a metal electrode from the skin; the metal grid copper transparent electrode layer is formed on the surface of the transparent flexible substrate layer through a yellow light etching micro-nano processing technology; the conductive gel layer uniformly covers the surface of the metal grid copper electrode layer; the encapsulation layer is formed by spin-coating a polymethyl methacrylate solution on the surface of the conductive gel layer and forming a transparent protection layer after curing; the gradient line width bionic honeycomb-shaped copper grid design is adopted, the light transmittance is kept, meanwhile, the collection efficiency of physiological electric signals is remarkably improved, signal transmission loss is reduced, through the polyacrylamide and nanocellulose composite gel formula and micro-channel dynamic humidity adjustment, signal stability under long-term wearing is ensured, and the application range is wide. The problem of signal distortion caused by dryness of traditional gel is solved.

The invention relates to a protein-based phenylboronic acid ester dynamic crosslinking hydrogel material as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving a component A, namely a phenylboronic acid modified protein polymer derivative, in a biocompatible medium to obtain a solution A; dissolving a component B, namely a macromolecular derivative containing a vicinal diol group, into a biocompatible medium to obtain a solution B; and mixing the solution A and the solution B with a certain concentration for a certain time to obtain the protein-based phenylboronic acid ester dynamic crosslinking hydrogel material. The boric acid ester dynamic cross-linking bond belongs to reversible chemical cross-linking reaction, and the prepared protein-based hydrogel material has enough mechanical strength and cheap operability. The protein-based hydrogel material disclosed by the invention can provide the effects of cell adhesion, proliferation and differentiation promotion, tissue regeneration and the like, can load and slowly release beneficial components for promoting tissue repair, is an ideal tissue engineering scaffold material, and can be applied to defect regeneration and repair of soft tissues, cartilages, bones and the like.

The invention relates to a long-term implantable gel composite woven medical catheter and a forming method thereof, the long-term implantable gel composite woven medical catheter comprises an inner film and a gel layer which are sequentially arranged from inside to outside, and a woven body is embedded in the gel layer. Compared with the prior art, the invention has the following beneficial effects: by introducing the PTFE inner layer, the reinforced braid layer and the polymer gel outer layer, perfect combination of internal lubrication, mechanical reinforcement and external functionalization of the catheter is realized; the novel method combining extraction, rapid cooling and vacuum freeze drying is adopted, the production efficiency is improved, the outer layer of the catheter is endowed with a unique microstructure, and the drug loading capacity and biocompatibility of the catheter are enhanced.

The invention discloses a flexible force-induced luminescence microstructure optical waveguide and a preparation method thereof. The flexible mechanoluminescence microstructure optical waveguide comprises an inner-layer fiber core, n mechanoluminescence composite blocks tightly attached to the inner-layer fiber core, a wrapping layer wrapping the inner-layer fiber core and the n mechanoluminescence composite blocks, and a protection layer coating the surface of the wrapping layer. The n mechanoluminescence composite blocks generate fluorescence signals with different colors under the action of external stress, the fluorescence signals are collected through the inner-layer fiber core and the cladding of the optical waveguide and are transmitted to a far end along the inner-layer fiber core to be detected and demodulated, the sensing section corresponding to stress is determined through the demodulated color, and the corresponding stress is determined through the luminous intensity. The composite material is good in biocompatibility, integrates sensing and transmission functions, and can be used in human body wearable equipment. According to the preparation method, different structures can be prepared in the optical waveguide layer by layer in a mode of customizing different molds, and different purposes are met by integrating different materials.

The embodiment of the invention relates to the technical field of brain-computer interfaces, in particular to a brain-computer interface module, a digital skull implant and an integration method and related application of the digital skull implant. The digital skull implant comprises a skull prosthesis and a brain-computer interface module, wherein the brain-computer interface module and the skull prosthesis are integrated; the brain-computer interface module comprises an ultrasonic transducer used for sending ultrasonic signals to the brain and collecting brain echo signals. The brain-computer interface module comprises an ultrasonic transducer used for sending ultrasonic signals to the brain and collecting brain echo signals. The brain-computer interface module is configured to be integrated with a skull or a skull prosthesis. In this way, the implantation structure integrating the brain-computer interface module in the skull form can have good mechanical strength and biocompatibility, complete implantation and wireless design can be achieved, and ultrasonic waves penetrate through the skull to conduct precise stimulation on the brain area.

The invention relates to a composition of polyester particles and sodium hyaluronate, which is prepared by the following steps: preparing PCL (Polycaprolactone) microspheres with the particle size of 20-50 microns by an emulsion method; hA is selected, and a PBS buffer solution is used for preparing an HA solution; repeatedly freezing and thawing in a refrigerator at-80 DEG C for 3 times to form micro-crosslinked HA gel; and mixing the microspheres and the micro-crosslinked HA gel according to a mass ratio of 25: 75. The invention provides a technology of taking the physically micro-crosslinked HA as a suspension carrier, so that the polyester particles or microspheres can be stably suspended in an HA solution system without a chemical crosslinking agent, and an injection product has better biocompatibility and safety. HA which does not contain a chemical cross-linking agent and polyester particles / microspheres are mixed to serve as a medical beauty filling system, and the problem of stable suspension of the polyester particles / microspheres in the HA is solved by performing physical micro-crosslinking on the HA.

The invention discloses a hydrogel dressing with a double-slow-release effect as well as a preparation method and application of the hydrogel dressing. The preparation method of the hydrogel dressing comprises the following steps: firstly, preparing methyl acrylate modified hyaluronic acid capable of being photo-crosslinked, then preparing carboxymethyl chitosan nanoparticles loaded with chlorogenic acid, and finally, mixing the methyl acrylate modified hyaluronic acid and the carboxymethyl chitosan nanoparticles loaded with chlorogenic acid to obtain the hydrogel dressing. The hydrogel dressing embedded with the nanoparticles is obtained in a photo-crosslinking mode. The hydrogel dressing provided by the invention not only has good biocompatibility and mechanical properties, but also plays an anti-oxidation role by removing free radicals in a body, and can effectively kill bacteria such as escherichia coli and staphylococcus aureus to play an antibacterial role. The hydrogel dressing can also reduce excessive inflammatory response in the wound healing process by influencing macrophage polarization, promote chronic wound healing and relieve pain of patients.

The invention discloses a rapid hemostasis powder material for massive hemorrhage. The rapid hemostasis powder material for massive hemorrhage is tannic acid modified carboxymethyl chitosan. The invention also provides a preparation method and application of the rapid hemostasis powder material for massive hemorrhage, and a rapid hemostasis microgel composition containing the rapid hemostasis powder material for massive hemorrhage. The rapid hemostasis powder material and microgel composition for massive hemorrhage have excellent water solubility, hemostasis performance and biocompatibility, have multiple effects of sterilization, anti-inflammation, wound healing promotion and the like, and are beneficial to rapid healing of wound parts after hemostasis.

The invention belongs to the technical field of medical cosmetology and plastic surgery, and particularly relates to a hydroxyapatite microsphere facial skin filling agent for injection and a preparation method thereof. Hydroxyapatite microspheres and sodium hyaluronate are uniformly mixed, a cross-linking agent is added, and cross-linked sodium hyaluronate-hydroxyapatite core-shell structure microsphere particles which take hydroxyapatite microspheres as cores and cross-linked sodium hyaluronate as shells and are of a soft-in-hard structure can be obtained through dialysis and particle grading. The cross-linked sodium hyaluronate is used as a core-shell structure, so that the hydroxyapatite is locked in the cross-linked sodium hyaluronate, agglomeration is effectively avoided, the extrusion resistance can be well reduced by the soft-wrapped-hard core-shell structure, and the propulsive force is smaller. All the components in the hydroxyapatite microsphere facial skin filler for injection have better biocompatibility, so that the rejection of the filler in a body is reduced, and the skin problems of allergy, redness, swelling, small lumps and the like after injection and filling are effectively avoided.

According to some embodiments, a patch for organ and / or tissue application is provided which comprises a biocompatible polymeric material having an auxetic architecture and further matching a stiffness ratio and / or Poisson's ratio of an organ or tissue to which the patch is applied. According to some further embodiments, biocompatible patches and methods of making the same are provided comprising providing a layer of hydrogel and forming an auxetic architecture by selectively crosslinking areas of the hydrogel via exposure to light, wherein the auxetic architecture is selected to match a stiffness ratio and Poisson's ratio of an organ to which the biocompatible patch is applied.

The invention belongs to the technical field of electrode materials, and particularly relates to a flexible liquid metal electrode based on gallium-indium-tin alloy and a preparation method and application thereof.Liquid metal ink is prepared through an ultrasonic method, a screen printing technology is adopted, the liquid metal ink is coated on a flexible substrate, meanwhile, mechanical activation and nanoparticle strategies are introduced, and the flexible liquid metal electrode based on gallium-indium-tin alloy is prepared. The preparation method of the high-performance flexible liquid gold electrode with excellent conductivity and mechanical stability is developed. In the flexible liquid metal electrode based on the gallium-indium-tin alloy, due to the good conductivity and biocompatibility of gallium-based liquid metal, the flexible stretchable conductive electrode based on the gallium-indium-tin liquid metal becomes an ideal wearable flexible device circuit material, and the patterning ability of the gallium-indium-tin liquid metal is further improved. Patterning preparation of the circuit is carried out in a mode of silk-screen printing of the gallium-based liquid metal particles, and the patterned liquid metal circuit with excellent conductivity and flexibility is obtained through mechanical activation.

The invention discloses a hydrogel patch for gastric perforation repair and a preparation method thereof, and belongs to the technical field of biomedical materials. The preparation method comprises the following steps: dissolving acryloyl valine, acrylic acid, methylene bisacrylamide, a compound A and a photoinitiator in an alcohol solvent, and defoaming to obtain a precursor solution; and placing the precursor solution in a mold, carrying out ultraviolet-induced curing, soaking the obtained hydrogel in a hydrochloric acid solution, and replacing the alcohol solvent to obtain the hydrogel patch for gastric perforation repair. The hydrogel patch provided by the invention has the advantages of strong acid corrosion resistance, good biocompatibility, low swelling ratio, stable adhesion performance in a fluctuating pH environment and the like. The tough and stable adhesion performance ensures that the hydrogel can be permanently and reliably adhered to a gastric perforation part in a dynamic gastric acid environment, and a stable platform is provided for drug delivery, so that the surgical operation time is expected to be shortened, and wound healing is promoted.

The invention relates to the technical field of medical instruments, and discloses an implantable neural electrode for long-term electrophysiological signal monitoring and a preparation method and application thereof. The neural electrode comprises an upper hydrogel insulation packaging layer, a lower hydrogel insulation packaging layer, an electrode site, a connecting line and a bonding pad, wherein the electrode site, the connecting line and the bonding pad are arranged between the upper hydrogel insulation packaging layer and the lower hydrogel insulation packaging layer, and the electrode site and the bonding pad are connected through the connecting line. The electrode sites, the connecting lines and the bonding pads are made of conductive hydrogel; the upper hydrogel insulation packaging layer and the lower hydrogel insulation packaging layer are made of insulation polymers and hydrogel. The insulating polymer is a plasma surface-modified insulating polymer. The preparation method of the neural electrode is simple and efficient, biotoxic materials are not introduced in the preparation process, good biocompatibility is achieved, continuous monitoring in a living body can be achieved for one year, important application value is achieved for studying signal response and neural circuits of all brain regions, and a beneficial tool is provided for long-term in-vivo monitoring.

The invention discloses a vascular endothelial growth factor (VEGF) and antibacterial peptide (AMP) loaded chitosan methacrylic acid (CSMA) hydrogel microneedle patch as well as a preparation method and application thereof, the microneedle patch takes CSMA hydrogel as a main body material, the CSMA hydrogel is crosslinked and cured through ultraviolet light, and the microneedle patch has the characteristics of simplicity and convenience in preparation and stable material performance. The microneedle patch can continuously and stably release VEGF and AMP, promote wound healing and inhibit bacterial infection. Experiments show that the hydrogel microneedle is non-toxic to cells, has good biocompatibility and biodegradability, and also has excellent antibacterial performance. The invention provides an efficient and safe novel treatment tool for repairing infectious wounds.

The invention relates to a low-molecular-weight chondroitin sulfate composition as well as a preparation method and application thereof, and aims to solve the problems that a chondroitin sulfate product obtained by a single degradation mode is limited in function and difficult to synergistically regulate intestinal barriers. The preparation method comprises the following steps: firstly, preparing LMCSO through oxidative degradation; secondly, preparing LMCSH through hydrothermal degradation; and finally, the LMCSD is prepared through deamination degradation. Finally, the obtained LMCSO, LMCSH and LMCSD are compounded according to the weight ratio of 5: 3: 2, and the low-molecular-weight chondroitin sulfate composition LMCSs-C is obtained. The composition LMCSs-C prepared by the invention can synergistically improve the intestinal barrier function through multiple action mechanisms, not only shows an obvious intestinal barrier repair effect in vitro and animal models, but also has good biocompatibility and safety, and shows a wide application prospect in intestinal health maintenance and related disease intervention.

The invention provides an implantable nerve probe integrated with a biological protection layer, a preparation method and a system. The nerve probe sequentially comprises a bottom biological protection layer, a bottom flexible substrate layer, a conductive layer, a top flexible substrate layer and a top biological protection layer from bottom to top, the bottom biological protection layer and the top biological protection layer form a biological protection layer located on the outermost periphery of the nerve probe and are used for avoiding erosion of a damp and hot cerebrospinal fluid environment in brain tissue. The integrated biological protection layer located on the outermost periphery of the flexible nerve probe is adopted, corrosion of cerebrospinal fluid to the nerve probe can be inhibited in in-vivo application, the long-term biocompatibility of the flexible nerve probe is effectively improved, the in-vivo long-term service life of the flexible nerve probe is effectively prolonged, and the nerve probe has the advantages of being high in integration level, high in stability and the like; and the requirement of in-vivo long-term effective service of the device can be met.

The invention discloses a near-infrared fluorescent probe for detecting ONOO <-> as well as a preparation method and application of the near-infrared fluorescent probe, and belongs to the technical field of peroxynitroso ion detection. The near-infrared fluorescent probe for detecting ONOO <-> provided by the invention is high in detection speed, and the highest fluorescence intensity can be reached within 60 seconds; the reaction is obvious and easy to observe, when the compound reacts with ONOO <->, strong red fluorescence can be generated, and the change of the color of the solution can be observed by naked eyes; the selectivity is strong, the sensitivity is high, the interference of other biological molecules and active substances can be effectively avoided, the accuracy and the reliability of a detection result are ensured, and the lowest detection limit can reach 107nM; the hydrogel has good biocompatibility, is low in toxicity in a living body, and does not generate adverse effects on normal physiological functions of a biological sample; the provided preparation method is simple and easy to operate, the raw materials are low in cost and easy to obtain, the synthesis efficiency is high, and the preparation method can be used for large-scale industrial production and has wide application prospects.

The invention belongs to the technical field of flexible sensing, and particularly relates to a preparation method of an implantable flexible sensor and a bladder urine content wireless monitoring system based on the sensor and a radio frequency mechanism. Wherein the performance of the sensor is improved by utilizing a microporous structure, n-octane is added into TPU to form a porous dielectric layer through a method of different temperatures and different liquid solubility, and the second dielectric layer is added into TPU by using a core-shell structure of silver nanowires and titanium dioxide, so that the anti-interference performance of the pressure-volume sensor is remarkably improved. In addition, the overall structure of the sensor is designed into a semi-bent snake-shaped structure, and the sensor has good pressure-volume performance, sensitivity, restorability and anti-interference performance. The elastic silicone rubber is used for packaging, so that the biocompatibility and practicability of the material are improved, and unstable factors such as body fluid invasion are eliminated. Meanwhile, a wireless sensing system based on a radio frequency mechanism is designed and manufactured, the urine content of the bladder can be wirelessly known from the outside, and related work such as medical care can be helped.

The invention discloses a two-dimensional bionic nanostructure assembled based on receptor-ligand acting force and a preparation method of the two-dimensional bionic nanostructure. The core characteristic of the two-dimensional bionic nanostructure is that the ultrafine particles modified by ligand molecules are anchored on the surface of a cell membrane fragment with a corresponding receptor expressed on the surface through a receptor-ligand effect, so that uniform compounding of the ultrafine particles and the cell membrane is realized; the bottlenecks of low compounding efficiency, functional site masking and the like caused by high surface curvature of small-size particles in the traditional cell membrane complete wrapping technology are broken through. According to the preparation method disclosed by the invention, the binding force between the cell membrane and the ultramicro particles is stronger, the off-target phenomenon of the ultramicro particles in the in-vivo circulation process can be avoided, the response release of the ultramicro particles under the competition effect in a lesion region expressed by a high-abundance receptor can be realized, and the preparation method has a good application prospect in the aspect of precise drug delivery. Meanwhile, the in-vivo circulation time of the ultrafine particles is prolonged by membrane loading, the biocompatibility of the ultrafine particles is improved, and the effectiveness and low toxicity of a drug delivery system are ensured.

The invention discloses protein-based nanoparticles as well as a preparation method and application thereof. The protein-based nanoparticles have a core-shell structure, the core is zein particles loaded with active drugs, and the shell comprises a trypsin inhibitor. The preparation method of the protein-based nanoparticles comprises the following steps: 1) preparing a zein dispersion liquid loaded with an active drug and a shell material aqueous solution; and 2) slowly adding the zein dispersion liquid loaded with the active drug into the shell material aqueous solution in a stirring state, and then carrying out rotary evaporation and freeze drying. The protein-based nano-particle has an excellent gastrointestinal digestion resisting effect, various hydrophobic active drugs can be effectively delivered to a lower digestive system, the stability and slow release efficiency of the loaded drugs are remarkably enhanced, and the protein-based nano-particle is good in biocompatibility, safe, non-toxic, simple in preparation process and suitable for industrial production. The method is suitable for large-scale industrial production and application.

The invention relates to bionic bio-ink as well as a preparation method and application thereof, and relates to the technical field of 3D bio-printing. The preparation method of the bionic bio-ink comprises the following steps: sequentially adding methylacryloylated recombinant human collagen, o-nitrobenzyl modified methylacryloylated hyaluronic acid, elastin-like polypeptide and phenyl-2, 4, 6-trimethylbenzoyl lithium phosphonate into a solvent, stirring and uniformly mixing to obtain an ACEM hydrogel precursor solution, and carrying out ultrasonic treatment on the ACEM hydrogel precursor solution to obtain the bionic bio-ink. Uniformly mixing the ACEM hydrogel precursor solution with a photoresist and seed cells to obtain the bionic biological ink. The sequence of the elastin-like polypeptide is as shown in SEQ ID NO. 1. The biological ink is better in mechanical strength, retains the advantages and functions of natural protein, can be quickly photo-cured into a high-strength and dual-network hydrogel structure, and has excellent printability, biocompatibility and cartilage tissue regeneration promoting capacity.