236 results about "Tissue engineered" patented technology

The invention discloses a biphasic hydrogel scaffold of a bionic bone cartilage matrix as well as a preparation method and application of the biphasic hydrogel scaffold, and belongs to the technical field of tissue engineering. The biphasic hydrogel scaffold of the bionic bone cartilage matrix provided by the invention comprises a hard bone matrix phase and a cartilage matrix phase. Wherein hard chitosan hydrogel with a porous structure is used as a hard bone matrix phase, and soft chitosan hydrogel is used as a cartilage matrix phase. The biphase hydrogel with the asymmetric matrix stiffness and the biochemical microenvironment can effectively release active substances in a controlled mode, the release period exceeds 21 days, the time requirement for osteochondral repair is met, and lasting support is provided for proliferation and differentiation of cells. Meanwhile, the biphase hydrogel provided by the invention has good biological safety, two matrix phases have different mechanical properties, the biphase hydrogel adapts to in-vivo complex physiological and mechanical environments, the fate of stem cells can be directionally regulated and controlled, synchronous regeneration and repair of osteochondral tissues are realized, and the biphase hydrogel has a wide application prospect.

The invention provides a piezoelectric composite stent, a preparation method thereof and application of the piezoelectric composite stent in tracheal defect repair, and belongs to the technical field of tissue engineering. The preparation method of the piezoelectric composite stent comprises the following steps: (1) immersing a PLLA stent into an FF polypeptide solution to prepare a PLLA-FF stent; (2) the PLLA-FF stent is immersed in a tannic acid solution, and a PLLA-FF-TA composite stent is prepared; (3) preparing oxygen-containing PLGA (poly (lactic-co-glycolic acid)) nano microbubbles, and mixing the GelMA with the oxygen-containing PLGA nano microbubbles to obtain a Gel-NB hydrogel precursor; (4) injecting the Gel-NB hydrogel precursor into the PLLA-FF-TA composite scaffold, and carrying out ultraviolet light cross-linking on the Gel-NB hydrogel precursor and the PLLA-FF-TA composite scaffold; and carrying out ultrasonic irradiation to obtain the PLLA-FF-TA (at) Gel-Nb + US composite scaffold. The piezoelectric hydrogel stent is successfully prepared, can be well used for tracheal defect repair, has the advantages of good degradation performance, high piezoelectric performance, long-term stability and high strength, and is beneficial to clinical transformation and application.

The invention discloses a recombinant human IV-type collagen as well as a preparation method and application thereof, and belongs to the field of bioengineering and protein engineering. According to the invention, three recombinant human IV type collagen new molecules are designed and screened on the basis of a part of sequences in a human IV type collagen alpha1 chain, soluble expression of a target protein is realized by using an escherichia coli system, and the recombinant collagen with the purity of more than 98% can be obtained through nickel column purification and acid regulation treatment. The recombinant collagen provided by the invention has good cell migration promotion activity and cell adhesion activity, and can be widely applied to the fields of tissue engineering, medical equipment, cosmetics, health care products and the like.

The invention discloses a collagen-based composite material and a preparation method thereof. The invention belongs to the technical field of composite materials. The collagen-based composite material comprises the following components in parts by weight: 50-60 parts of collagen; 10 to 20 parts of nano cellulose; 20 to 30 parts of a reinforcing component; 1-3 parts of a cross-linking agent; and 0.5-2 parts of a functional additive. By adding the reinforcing component, the mechanical strength of the collagen-based composite material is remarkably improved, and meanwhile, the non-toxic cross-linking agent and the natural reinforcing component are adopted, so that the material has good biocompatibility and does not generate toxic or adverse reaction on cells and tissues; according to the present invention, the porous three-dimensional network structure is provided, the pore size is 50-300 [mu] m, the porosity is more than or equal to 80%, the cell adhesion, the proliferation and the nutrient substance transmission are easily achieved, and the method is suitable for tissue engineering and wound repair; according to the collagen-based composite material disclosed by the invention, through reasonable raw material formula design, the collagen, the nano cellulose and the natural polysaccharide are compounded, the advantages of all the components are fully exerted, and the comprehensive performance of the composite material is further improved.

The present invention relates to a modified pluripotent cell and to a method for forward programming said cell. The invention further relates to use of said modified pluripotent stem cell for tissue engineering and to a food product comprising said modified pluripotent stem cell.

The preparation method comprises the following steps: (1) fish skin pretreatment: (2) gas foaming treatment: weighing sodium borohydride with a certain molar concentration, dissolving the sodium borohydride in a phosphate buffer solution with a certain concentration, stirring until bubbles in the solution are obviously generated and most of solids are dissolved, and then, carrying out gas foaming treatment; putting the cleaned fish skin tissues in the step (2), and performing foaming treatment; (3) cleaning treatment; and (4) protection and storage. The gas foaming technology is innovatively adopted for decellularization treatment, the interface stripping effect between cells and a matrix is remarkably enhanced on the premise that the collagen three-dimensional structure and the scaffold morphology integrity are not damaged, and the method is suitable for the decellularization pretreatment process of soft tissue (such as fish skin and placenta) with a compact structure or cell-intensive type. The development of a biomedical material, especially a tissue engineering scaffold technology, is promoted, and the preparation method has a wide application prospect.

The invention belongs to the field of biomedicine, and particularly relates to a 3D bio-printed vascularization-promoting bladder patch and a preparation method thereof. The preparation method comprises the following steps: polymerizing prepared GelMA and Laponite to prepare GelMA-Laponite hydrogel, then mixing and printing the GelMA-Laponite hydrogel and seed cells on a bladder acellular matrix by using a 3D biological printing machine to form a GelMA-Laponite stent with a three-dimensional space structure, and crosslinking the stent by using ultraviolet light to obtain the double-layer vascularized bladder patch. The construction level of the tissue-engineered bladder patch is expected to be improved, the vascularization problem of the tissue-engineered bladder graft is solved, and a good theoretical basis and technical support are laid for promoting the clinical application process of bladder tissue engineering.

The invention is applicable to the technical field of hydrogel, and provides dynamic cross-linked hydrogel and a preparation method and application thereof, and the preparation method comprises the following steps: cross-linking oxidized dextran and carboxymethyl chitosan containing phenylboronic acid modification groups; glucanase is added before the hydrogel is formed, and calcium peroxide nanoparticles coated with polydopamine are introduced; wherein the mass concentration of the oxidized dextran is 2-10% w / v, the mass concentration of the carboxymethyl chitosan containing the phenylboronic acid modified group is 2% w / v, the concentration of the glucanase is 10-100 U, and the concentration of the calcium peroxide nanoparticles coated with the polydopamine is 0.1-1% w / v. The hydrogel disclosed by the invention shows good cell compatibility, angiogenesis promoting capability and bioactivity for promoting dental pulp regeneration in multiple in-vitro and in-vivo experiments, is suitable for tissue engineering fields such as dental pulp injury repair and the like, and effectively solves the technical problem that conventional hydrogel is insufficient in cell survival support in three-dimensional culture and the like.

The invention belongs to the technical field of cell mechanics biology experiment devices for health and rehabilitation engineering, and relates to a microfluidic device for endothelialization evaluation of a tissue engineering valve, which comprises a microfluidic chip, peripheral equipment, a signal acquisition and processing system and a feedback system. The biomechanical principle and the micro-fluidic chip technology are ingeniously combined, the control system, the mechanical loading device and the micro-fluidic chip are connected, a micro-fluidic endothelial cell culture cavity and a peripheral system are constructed, real-time accurate control over aortic valve blood flow is achieved through the PID control system, and the system is suitable for large-scale popularization and application. Shear stress applied to a tissue engineering valve endothelial cell culture area is generated, so that the real stress condition of the tissue engineering valve endothelial cells is simulated, and the method is used for analyzing and researching the influence rule of mechanical loading on the tissue engineering valve endothelial cells.

The invention belongs to the crossing field of biomedical engineering, material science and tissue engineering, and discloses multifunctional hydrogel capable of switching mechanical and adhesive properties based on temperature triggering as well as a preparation method and application of the multifunctional hydrogel. The water-based adhesive is prepared from acrylic acid, gelatin, hexadecyl trimethyl ammonium bromide, silver nanoparticle-loaded polydopamine nanoparticles and water. According to the present invention, the good adhesion property and the good mechanical property are provided for the skin tissue, the mechanical property and the adhesion property can be switched, and the adhesion property durability and the adhesion property repeatability are achieved. The temperature-triggered hydrogel prepared by the method has the advantages of simple process, low cost and no introduction of toxic chemical substances, can achieve the effects of tight fitting at body temperature and natural falling at low temperature according to the driving of temperature, still has excellent adhesion performance in a complex environment, and promotes the development of intelligent medical multifunctional wound dressings.

The invention provides autologous platelet-containing plasma gel and a preparation method and application thereof, and relates to the technical field of biological medicine and tissue engineering.The preparation method comprises the following steps that peripheral venous blood of a patient is extracted and added into a centrifugal tube mixed with a sodium citrate anticoagulant; putting the centrifugal tube into a centrifugal machine for centrifuging, and taking upper-layer plasma; wherein the upper-layer plasma refers to plasma between the upper-layer liquid level and the lower-layer red blood cell layer by 1mm; the centrifugal speed ranges from 1300 r / min to 1700 r / min; the centrifugation time is 3-5 min; under a sterile condition, preparing a calcium gluconate / thrombin mixed solution; and dropping the calcium gluconate / thrombin mixed solution into the upper plasma, mixing, and solidifying to obtain the autologous platelet-containing plasma gel. After one-time centrifugation, upper-layer full-layer plasma is extracted, the volume of the prepared gel is far larger than that of a traditional method, the utilization rate of whole blood platelets and fibrinogen is close to 100%, and regeneration and repair of large-area tissue wounds can be met.

The present invention relates to methods for obtaining induced smooth muscle cells (iSMCs), iSMCs, iSMCs for use in a method of treating a disease or disorder or for use in tissue engineering, and the use of skeletal muscle derived cells for obtaining iSMCs.

The invention relates to a nerve repair guide catheter, a preparation method and application, an electrostatic spinning technology is adopted to construct a nanofiber membrane which is directionally and orderly arranged so as to guide nerve axons to directionally grow, and cell energy metabolism is promoted and tissue repair and regeneration are accelerated through metabolism and conversion of degradation products. Therefore, the nerve injury repairing effect better than that of traditional matrix materials without biological activity, such as PCL, is achieved. According to the invention, a novel solution is provided for solving the problem of insufficient biological activity of a traditional nerve conduit material by constructing a degradable high polymer material with biological activity. Therefore, the regeneration efficiency of peripheral nerve injury repair is improved, and the application prospect of the bioactive material in the fields of tissue engineering and regenerative medicine is expanded.

The invention relates to a tissue engineering trachea, which combines an additive manufacturing technology with a biological material, simulates the form and the function of a natural gas pipe, designs a cartilage-fiber alternating ring structure and a bionic fiber band structure, and designs a unique blood supply channel in a fiber section. The trachea fiber section is composed of hydrogel with a hierarchical porous structure, and a circumferential blood vessel channel and a radial blood vessel pore channel are designed in the hydrogel so as to promote ingrowth and growth of new blood vessels and optimize the blood supply reconstruction process. The tracheal cartilage section is made into a C-shaped cartilage ring with cartilage cells through a biological printing technology, and necessary mechanical support is provided. The fiber segment and the cartilage segment are effectively integrated through chemical bonds of functional groups to ensure stable connection of the two. Partitioned construction of fibers and cartilage is achieved through the biological 3D printing technology, the bionic tissue engineering trachea is formed in a modular construction-integrated assembly mode, it is verified that repairing and reconstruction of multiple types of tissue bionic tracheas can be achieved, and an innovative solution is provided for tracheal injuries.

The present disclosure provides a hydrogel composition for tissue engineering and regenerative medicine, including naturally derived polymers functionalized with furan groups and nanoparticles of poly(lactic-co-glycolic acid)-poly(ethylene glycol) copolymer functionalized with maleimide groups. Covalent crosslinking via a bioorthogonal Diels-Alder click reaction forms a tunable, biocompatible, and biodegradable three-dimensional network. The hydrogel composition mimics the extracellular matrix and offers adjustable mechanical properties, controlled biodegradation, and therapeutic agent delivery for applications such as cartilage regeneration and drug delivery.

The invention belongs to the field of biomedical materials and tissue engineering, and particularly relates to an injectable mineralized collagen bone repair composite material loaded with stem cell recruitment factors and osteogenic induction factors and a preparation method of the injectable mineralized collagen bone repair composite material. The composite material is composed of 50%-53% of mineralized collagen matrix particles, 15%-20% of SDF-1alpha loaded heparinized gelatin microspheres, 15%-20% of BMP-2 loaded PDA coated mesoporous silica particles, 8%-10% of an NHS-PEG-NHS cross-linking agent and 4%-6% of glycerin. The composite material can realize time-space sequential regulation and control of'collection first and then differentiation '; a bionic bone matrix microenvironment is provided through the mineralized collagen matrix; the problems of two-factor release conflict and stability are solved; in-vitro experiments show that the material can significantly promote stem cell migration and osteogenic differentiation, and animal experiments prove that the material can recover 82-88% of mechanical properties of bone defect parts. The invention solves the technical problems of insufficient stem cell collection, low osteogenic differentiation efficiency and uncontrollable factor release of the existing bone repair material.

The present disclosure provides solid collagen constructs and tissue compositions, wherein a polymerizable collagen solution or suspension is extruded in the presence or absence of cells to formed an aligned architecture comprising solid collagen constructs such as those made with fibrillar collagen. Methods of using and of manufacturing solid collagen constructs and tissue compositions, where the component collagen is solid fibrillar collagen and cells are preferentially aligned, are also provided.

The present invention is concerned with a manufactured article prepared from a biomaterial comprising isolated and purified type I collagen fibrils derived from abalone and a method of preparing such an article. The manufactured article can be prepared by 3D bioprinting, and therefore the present invention also envisages a novel bioink for a 3D bioprinter comprising an isolated and purified type I collagen fibril and cartridges containing such a bioink. The invention additionally relates to a method of isolating type I collagen fibrils from abalone. The manufactured article is useful in a number of biomedical applications including as an implantable device where it can function as a 3D bioprinted hard or soft tissue scaffold for tissue engineering.

The present disclosure describes systems and methods for cell therapy. Cells (e.g., stem cells, immune cells, cardiomyocytes, etc.) may be engineered to exhibit extended half-lives as compared to control cells (e.g., non-engineered cells). The cells may be engineered to exhibit increased proliferative activity as compared to control cells. In some embodiments, immune cells may be engineered to efficiently and specifically target diseased cells (e.g., cancer cells) while control cells are insufficient or unable to target the diseased cells. In some embodiments, stem cells or differentiated cells, such as myocardial cells, may be administered to a target tissue for enhanced tissue engineering or regenerative medicine. The engineered cells disclosed herein can be engineered ex vivo, in vitro, and in some embodiments in vivo. Engineered cells prepared ex vivo or in vitro may be administered to a subject in need thereof to treat a disease (e.g., myeloma or solid tumor). The engineered cell may be autologous to the subject. Alternatively, the engineered cell may also be allogeneic to the subject.

The present invention provides constructs including a tubular biodegradable polyglycolic acid scaffold, wherein the scaffold may be coated with extracellular matrix proteins and substantially acellular. The constructs can be utilized as an arteriovenous graft, a coronary graft, a peripheral artery bypass conduit, or a urinary conduit. The present invention also provides methods of producing such constructs.

The invention relates to a heat and light sensitive tissue scaffold suitable for the growth, proliferation and organization of the cells in the technical field of tissue engineering, which is endowed with intelligent behavior and thus has a dynamic structure, and a method for producing the same.

The invention discloses a rotary incubator suitable for multiple tissue engineering materials, and belongs to the technical field of experimental equipment. According to the device, a culture tube is placed in a clamping sleeve at a corresponding position, at the moment, a second electric push rod can be controlled to work and shorten to pull a sliding rod to move, the sliding rod can move to drive a top pressing disc to move downwards, and the pressing disc can make contact with the top of a container when moving downwards, so that the container can be clamped and limited; if an opening in the top of the container is not provided with a sealing cover, the opening of the container can be sealed through pressing of the pressing disc, and the container can move along with the clamping sleeve when the driving mechanism drives the container to be shaken up through axial abutting of the clamping sleeve and the container, so that the situation that the container topples over and leaks liquid due to circumferential shaking-up movement is avoided, and the shaking-up processing stability is improved; the heater main body on the rear side can perform constant-temperature treatment through air blowing or heat radiation, so that the placement and culture effects are improved.

Some embodiments relate to nanoparticle probes for the detection of disease states in a patient or for tissue engineering. In some embodiments, the nanoparticle probe comprises one or more slip bonds that bind to a cell surface structure. In some embodiments, the binding of the nanoparticle probe is selective. In some embodiments, the nanoparticle probe binds to cells having a certain maximum glycocalyx thickness.

The invention provides a tissue engineering heart valve stent with a sulfonated nano-enzyme coating as well as a preparation method and application of the tissue engineering heart valve stent, and belongs to the technical field of medical materials. The invention relates to a tissue engineering heart valve stent with a sulfonated nano-enzyme coating, which comprises an acellular matrix carrier, ruthenium / iridium-tannic acid nano-enzyme loaded on the acellular matrix carrier, and a sulfonated polymer coating fixed on the acellular matrix carrier and / or the surface of nano-particles through ultraviolet polymerization, the ruthenium / iridium-tannic acid nano-enzyme is formed by tannic acid TA, Ru < 3 + > and Ir < 3 + > through coordination. According to the invention, through double bionic activities of superoxide dismutase and nitric oxide synthase of ruthenium / iridium-tannic acid nano-enzyme and through the super-hydrophilicity and electrically neutral surface of the sulfonated polymer coating, collaborative regulation and control of O2-NO cascade catalysis and interface functions in a pathological microenvironment are realized; the structural and functional in-situ regeneration of the tissue engineering heart valve stent is promoted.

The invention discloses a high internal phase emulsion with a nested structure, a preparation method thereof and a porous material, the high internal phase emulsion has at least one layer of nested structure, the nested structure is an HIPE-1 / HIPE-2 structure, the HLB value of an emulsifier A used by the HIPE-1 is recorded as H1, the HLB value of an emulsifier B used by the HIPE-2 is recorded as H2, H1 and H2 meet the condition that H1 is greater than H2, and the difference between H1 and H2 is 8-12; according to the preparation method disclosed by the invention, the emulsifying agents in the HIPE-2 emulsion and the HIPE-1 emulsion are controlled to have a certain difference value, so that the interface structures of at least two different HIPE emulsions in the obtained high-internal-phase emulsion are stable and are immiscible with each other, and the finally obtained porous material has multistage pore diameters and provides a bionic carrier for tissue engineering, drug screening and the like.

The invention relates to the technical field of biomedical materials and tissue engineering, and discloses a preparation method and application of adjustable porous fat foam. Comprising the following steps: fat tissue pretreatment and freeze-thaw circulation, multi-step combined decellularization and impurity removal, graded washing and purification, freeze grinding and freeze drying, preparation of a decellularized fat matrix suspension, porous foam scaffold forming and foam scaffold gradient rehydration. By adopting a multi-step combined decellularization and impurity removal process, residual lipid droplets in adipose tissues can be actively extruded out by mechanical squeezing assisted by isopropanol degreasing, a lipid wrapping phenomenon is broken, meanwhile, residual nucleic acid and adipose components can be thoroughly removed by virtue of a synergistic degradation effect of nuclease and lipase, the immunogenicity risk is reduced, and the immunogenicity of the adipose tissues is improved. All the steps have a synergistic effect under strict control of temperature, concentration and time parameters, so that the structural integrity of the extracellular matrix is ensured, the decellularization efficiency and thoroughness are improved, and the biological safety and biocompatibility of a final product are ensured.

The invention belongs to the technical field of biological medicine, and particularly discloses a small molecule compound SR4370 for promoting hair follicle growth and regeneration and application thereof. High-throughput screening finds that SR4370 can induce dermal fibroblasts to be reprogrammed into DP-like cells with characteristics of hair papilla cells and up-regulate ALP, WNT5A and other marker genes, and in-vivo and in-vitro experiments prove that SR4370 can activate resting hair follicles, promote in-vitro hair follicle growth and enhance the regeneration capacity of dermal stem cells. The invention provides a safe and efficient hair follicle regeneration strategy, and provides a new tool for alopecia treatment and tissue engineering.

The invention discloses an edible beefsteak scaffold based on vegetable protein and a construction method thereof, and belongs to the fields of tissue engineering, material science, food science and biology. The edible beefsteak scaffold is a 3D printing scaffold based on vegetable protein, combined with gellan gum and sodium alginate, and is a cell culture meat bionic beefsteak composed of a bovine fibroblast and bovine muscle satellite cell co-culture system dynamically constructed by using a rotary culture system. The selected materials are all edible protein and polysaccharide, have high biocompatibility and safety, and are wide in source; the mode of dynamically constructing the bionic beefsteak can effectively reduce the formation of local necrotic areas, and the bionic beefsteak has excellent physical characteristics, has texture structures and flavor characteristics similar to those of real beefsteak, is sterile in surface, and can significantly reduce food sanitation and safety problems. The scheme provided by the invention has remarkable advantages in the aspects of food safety, texture regulation and control, sensory simulation and the like, and provides important technical support for the field of cell culture meat.

Described herein are bioengineered constructs and methods of producing the same. The constructs and methods disclosed herein can be applied towards, for example, the generation of vascular grafts to treat cardiovascular disease.

A novel portable, and attachable device allows precise positioning of micro-tissues, tumor spheroids, and other biological samples during bioprinting. The device features an interchangeable connector for handling samples of various sizes and uses a manually adjustable pressure system for suction and deposition. A programmable control unit coordinates pressure and positioning based on input commands, enhancing accuracy and consistency in tissue handling. Compatible with multiple bioprinter systems and supporting both manual and automated operation, it streamlines workflows and improves user experience. This device addresses challenges in high-throughput biological positioning, critical for tissue engineering, clinical, pharmaceutical, and research applications. It supports advancements in customized medicine, biological models, and environmental monitoring, meeting the growing demand for precise biologic handling in the multi-billion-dollar bioprinting and biopsy markets. The device offers superior control over tissue biopsy positioning compared to current manual devices reliant on operator skill.