105 results about "Tissue engineered" patented technology

The present disclosure provides a hydrogel composition for tissue engineering and regenerative medicine, including naturally derived polymers functionalized with furan groups and nanoparticles of poly(lactic-co-glycolic acid)-poly(ethylene glycol) copolymer functionalized with maleimide groups. Covalent crosslinking via a bioorthogonal Diels-Alder click reaction forms a tunable, biocompatible, and biodegradable three-dimensional network. The hydrogel composition mimics the extracellular matrix and offers adjustable mechanical properties, controlled biodegradation, and therapeutic agent delivery for applications such as cartilage regeneration and drug delivery.

The present invention provides constructs including a tubular biodegradable polyglycolic acid scaffold, wherein the scaffold may be coated with extracellular matrix proteins and substantially acellular. The constructs can be utilized as an arteriovenous graft, a coronary graft, a peripheral artery bypass conduit, or a urinary conduit. The present invention also provides methods of producing such constructs.

The invention discloses a rotary incubator suitable for multiple tissue engineering materials, and belongs to the technical field of experimental equipment. According to the device, a culture tube is placed in a clamping sleeve at a corresponding position, at the moment, a second electric push rod can be controlled to work and shorten to pull a sliding rod to move, the sliding rod can move to drive a top pressing disc to move downwards, and the pressing disc can make contact with the top of a container when moving downwards, so that the container can be clamped and limited; if an opening in the top of the container is not provided with a sealing cover, the opening of the container can be sealed through pressing of the pressing disc, and the container can move along with the clamping sleeve when the driving mechanism drives the container to be shaken up through axial abutting of the clamping sleeve and the container, so that the situation that the container topples over and leaks liquid due to circumferential shaking-up movement is avoided, and the shaking-up processing stability is improved; the heater main body on the rear side can perform constant-temperature treatment through air blowing or heat radiation, so that the placement and culture effects are improved.

The invention discloses a high internal phase emulsion with a nested structure, a preparation method thereof and a porous material, the high internal phase emulsion has at least one layer of nested structure, the nested structure is an HIPE-1 / HIPE-2 structure, the HLB value of an emulsifier A used by the HIPE-1 is recorded as H1, the HLB value of an emulsifier B used by the HIPE-2 is recorded as H2, H1 and H2 meet the condition that H1 is greater than H2, and the difference between H1 and H2 is 8-12; according to the preparation method disclosed by the invention, the emulsifying agents in the HIPE-2 emulsion and the HIPE-1 emulsion are controlled to have a certain difference value, so that the interface structures of at least two different HIPE emulsions in the obtained high-internal-phase emulsion are stable and are immiscible with each other, and the finally obtained porous material has multistage pore diameters and provides a bionic carrier for tissue engineering, drug screening and the like.

The invention relates to the technical field of biomedical materials and tissue engineering, and discloses a preparation method and application of adjustable porous fat foam. Comprising the following steps: fat tissue pretreatment and freeze-thaw circulation, multi-step combined decellularization and impurity removal, graded washing and purification, freeze grinding and freeze drying, preparation of a decellularized fat matrix suspension, porous foam scaffold forming and foam scaffold gradient rehydration. By adopting a multi-step combined decellularization and impurity removal process, residual lipid droplets in adipose tissues can be actively extruded out by mechanical squeezing assisted by isopropanol degreasing, a lipid wrapping phenomenon is broken, meanwhile, residual nucleic acid and adipose components can be thoroughly removed by virtue of a synergistic degradation effect of nuclease and lipase, the immunogenicity risk is reduced, and the immunogenicity of the adipose tissues is improved. All the steps have a synergistic effect under strict control of temperature, concentration and time parameters, so that the structural integrity of the extracellular matrix is ensured, the decellularization efficiency and thoroughness are improved, and the biological safety and biocompatibility of a final product are ensured.

A novel portable, and attachable device allows precise positioning of micro-tissues, tumor spheroids, and other biological samples during bioprinting. The device features an interchangeable connector for handling samples of various sizes and uses a manually adjustable pressure system for suction and deposition. A programmable control unit coordinates pressure and positioning based on input commands, enhancing accuracy and consistency in tissue handling. Compatible with multiple bioprinter systems and supporting both manual and automated operation, it streamlines workflows and improves user experience. This device addresses challenges in high-throughput biological positioning, critical for tissue engineering, clinical, pharmaceutical, and research applications. It supports advancements in customized medicine, biological models, and environmental monitoring, meeting the growing demand for precise biologic handling in the multi-billion-dollar bioprinting and biopsy markets. The device offers superior control over tissue biopsy positioning compared to current manual devices reliant on operator skill.

The invention relates to a functionalized cartilage tissue repair material as well as a preparation method and application thereof, and belongs to the technical field of tissue engineering. The invention aims to solve the problems that the existing decalcified bone matrix is insufficient in cartilage induction in cartilage repair, and is easy to cause blood vessel ingrowth and fibrosis repair. The preparation method comprises the following steps: inoculating cartilage cells on a three-dimensional porous frame structure carrier, carrying out in-vitro dynamic pre-culture to enable the cartilage cells to secrete an extracellular matrix, and carrying out decellularization treatment to obtain the functionalized cartilage tissue repair material retaining the cartilage specific matrix. The material has a suitable pore structure and abundant II-type collagen deposition, can effectively promote chondrocyte differentiation and hyaline cartilage regeneration, inhibits blood vessel ingrowth, maintains a cartilage vascular-free microenvironment, significantly improves the structure and function reconstruction effect of cartilage defect repair, and has good biocompatibility and clinical transformation potential.

The present application relates to a controllable degradation of siliconized DNA hydrogel and its preparation method and application, the hydrogel is assembled into a three-dimensional network through the DNA structure with single-stranded region in the center and linear DNA structure, and the mechanical properties and thermal stability thereof are improved through in-situ siliconization. The precisely designed single-stranded region can still be recognized and degraded by specific nucleic acid enzyme after siliconization, realizing the accurate control of selective degradation. Taking human umbilical vein endothelial cells as an example, the present application verifies the good biocompatibility of the material, and the present application can be used for cell embedding and release, and is suitable for advanced biological materials, medical, pharmaceutical and other research and application fields such as organoid culture, tissue engineering and controllable drug release.

The invention provides a high-transparency and high-simulation bacterial engineering biological cornea as well as a preparation method and application thereof, and belongs to the technical field of tissue engineering cornea materials. According to the method, a bacterial synthetic biology method is adopted, a curvature customization model and an hydroformylation system are combined, and the high-transparency bionic cornea (DBC-L-Cel) which is customized in curvature and shape and has a nanofiber network structure is constructed. The light transmittance of the bionic cornea reaches 91.91%, the light transmittance and the mechanical property of the bionic cornea are matched with those of a natural cornea, and meanwhile the bionic cornea has excellent biocompatibility, adhesiveness and scar resistance. In-vitro experiments show that DBC (at) L-Cel can reduce matrix fibrosis by inhibiting AGE / RAGE / NF [kappa] B and PI3K / AKT pathways and up-regulate Wnt / beta-catenin pathway to activate proliferation, and corneal epithelium is repaired by an adhesion function. In-vivo experiments show that the high-simulation biological cornea DBC-L-Cel is transplanted to a rabbit model with large-scale corneal defects, repair and regeneration of corneal stroma and epithelium can be remarkably promoted, and rapid scar-free repair and reconstruction of the damaged cornea are achieved.

Disclosed are implantable devices, systems, and methods of fabricating, testing, optimizing, and / or implanting such devices. An implantable device includes a body, a first intrinsic channel network formed in the body and configured to receive cells, and a second intrinsic channel network formed in the body and configured to allow for active flow of a fluid to support viability and / or function of the cells in the first intrinsic channel network. The first intrinsic channel network comprises a first channel and the second intrinsic channel network comprises a second channel, with at least segments of the first and second channels parallel to and spaced apart from each other. Cells may be suspended in a collagen matrix and introduced to the first intrinsic channel network.

The invention provides a shape memory nano short fiber stent with self-adaptive capacity and preparation and application thereof, and belongs to the technical field of tissue engineering materials. The preparation method comprises the following steps: firstly, preparing flexible polylactic acid-fullerol composite nanofibers containing uniformly dispersed fullerol nanocrystals by utilizing an electrospinning technology, then dividing the flexible polylactic acid-fullerol composite nanofibers into short fibers by virtue of homogenization and freeze-drying processes, and assembling the short fibers into a three-dimensional porous scaffold by taking chitosan as an adhesive. The porous scaffold material disclosed by the invention has excellent biological characteristics and a water-triggered shape memory effect, and due to the fact that chitosan has excellent antioxidant activity and inherent bone induction characteristics, the nanofiber scaffold with the controllable and recoverable shape developed by the invention is expected to effectively promote osteoporotic bone healing.

The Cardio-Tox Tissue Engineered Model (TEEM) invention provides a robust in vitro model for cardiotoxicity evaluation using three-dimensional (3D) human heart microtissues to quantify dose-dependent changes in electromechanical activity, resulting in a comprehensive cardiotoxicity and arrhythmia risk assessment of test compounds. The invention also provides a predictive in vitro screening platform for pro-arrhythmic toxicity testing using human three-dimensional cardiac microtissues. The invention enables the screening of environmental and pharmaceutical compounds, chemicals, and toxicants to establish safe human exposure levels.

The invention discloses a metal organic framework material targeting bone marrow fat cells as well as a preparation method and application of the metal organic framework material, and belongs to the technical field of biomedical materials and tissue engineering. The material comprises: (1) a ZIF-8 core structure used for loading nucleic acid / small molecule drugs; (2) surface modification of lipid droplet targeting peptide: the lipid droplet targeting peptide is combined with a PEDF receptor to realize precise targeting; and (3) anti-miR-188 is loaded inside and is used for inhibiting adipogenic differentiation. The method at least has the following advantages: (1) a bone marrow adipocyte specific targeting MOF material is proposed for the first time, and bone metabolism intervention is converted from a mode of targeting osteoblast or macrophage to targeting adipocyte; (2) the MOF core can quickly respond to a marrow weak acid environment to realize accurate release; (3) the AdipoPep targeting peptide can obviously prolong the residence time of the bone marrow; and (4) adipogenic differentiation is significantly inhibited, osteogenesis microenvironment reconstruction is promoted, and an excellent bone mass recovery effect is shown in an osteoporosis model.

The invention discloses a preparation method of a vascular organ chip based on a degradable bionic stent. The degradable biological scaffold (PCL-FBE scaffold for short) is obtained by mixing a swimming bladder extract and polycaprolactone and carrying out electrostatic spinning. On the basis, a vascular organ chip which can be integrated in a micro-fluidic system is further constructed, and a whole process for completing three-dimensional dynamic culture of vascular organs by using the chip is established. According to the present invention, the swim bladder extract and polycaprolactone are compounded to form the electrospinning scaffold, such that the defects of the mechanical property, the degradability and the biocompatibility of the traditional bionic scaffold are successfully overcome, the organoid culture effect is significantly improved, and the efficient and sustainable innovation platform is provided for the tissue engineering and the regenerative medicine.

The invention belongs to the technical field of tissue engineering, and particularly relates to a bio-ink system for osteochondral repair as well as a preparation method and application of the bio-ink system. The system comprises first bio-ink and second bio-ink which are respectively used for simulating cartilage and bone tissue. The first biological ink is prepared from methacrylic acid gelatin, acellular extracellular matrix powder, aldehyde modified natural polysaccharide, a photoinitiator and a transforming growth factor; the second biological ink is prepared from methacrylic acid gelatin, acellular extracellular matrix powder, nano-hydroxyapatite, aldehyde modified natural polysaccharide, a photoinitiator and human bone morphogenetic protein BMP-2. The bio-ink system can guarantee smooth extrusion and interlayer fusion in the printing process; the constructed bionic microenvironment and the affinity effect of the bionic microenvironment on growth factors are suitable for 3D printing construction of the osteochondral integrated bionic scaffold and have good clinical transformation potential.

The invention belongs to the technical field of biomedical materials and tissue engineering, and particularly relates to a self-pumping stent material with a double-layer heterogeneous structure as well as a preparation method and application of the self-pumping stent material. A hydrophobic membrane is prepared by an electrostatic spinning technology, and a hydrophilic microgel assembly is prepared by an emulsion method. Adding a dopamine modified polymer, and establishing a dynamic phenylboronic acid ester bond between microgel through a dopamine group to form a hydrophilic microgel assembly; the adhesion connection of the microgel assembly and the hydrophobic membrane is realized through mussel bionic chemical reaction of dopamine groups, and a double-layer heterogeneous structure based on the hydrophilic assembly and the hydrophobic membrane is constructed, so that a self-pumping effect is generated, and one-way flow of liquid components from the hydrophobic side of the stent to the hydrophilic side of the stent can be realized. Implanting the stent into an alveolar bone defect wherein the hydrophobic membrane contacts gingiva and the hydrophilic microgel assembly fills the bone defect; through the self-pumping effect of the stent, nutritional ingredients in the gingiva are unidirectionally pumped to the defect part of the alveolar bone, so that the regeneration of the alveolar bone is promoted.

The invention relates to an extracellular vesicle-frozen gel scaffold as well as an integrated preparation method and application thereof, and belongs to the technical field of biotechnology and tissue engineering materials. The problems that in the prior art, extracellular vesicles are prone to being damaged by low temperature in the frozen gel preparation process, a traditional cryoprotectant is not compatible with a stent process, and burst release and action time is short in a vesicle physical adsorption method are solved. The method comprises the following steps: mixing extracellular vesicles with a trehalose solution, and standing to obtain a vesicle-trehalose mixed solution; mixing with the support material prepolymer solution, and adding a photoinitiator to form a composite precursor solution; freezing the solution, and performing cross-linking solidification to form a three-dimensional porous gel scaffold; and unfreezing and washing to obtain the stent. The trehalose is introduced as a cryoprotectant, vesicle activity protection and stent structure forming are synchronously achieved in low-temperature forming, and the prepared stent can achieve controllable slow release of vesicles and has good application prospects in the field of tissue repair and regeneration.

The present application relates to the technical field of biomedical materials, in particular to a super-elastic hydrogel material, a super-elastic injectable scaffold and a preparation method and application thereof. The super-elastic hydrogel material is obtained by cross-linking a base material with an initiator; the base material comprises GelMA and PEGDA with a molecular weight of 2000-20000; the injectable scaffold comprises a three-dimensional structure scaffold composed of super-elastic material, which can restore the original shape after being compressed and injected. The present application utilizes the excellent mechanical properties and shape memory of super-elastic material to realize solid injection and restore the original shape after injection, thereby providing a new idea and approach for large-size injectable scaffolds. The scaffold of the present application can be used in the fields of tissue engineering and regenerative medicine, minimally invasive injection transplantation, artificial skin, myocardial patch, in-vitro engineered tissue model construction, tissue physiological function remodeling and pathological process mechanism research, mechanical regulation and stress regulation mechanism research, drug development and drug screening, etc.

This invention discloses a photo-acid synergistic enhancement bio-ink for extrusion 3D printing and its preparation method. The ink is composed of N-vinylpyrrolidone (NVP), polyvinyl alcohol (PVA), a photoinitiator, and malic acid with a unique "single-hydroxyl-dicarboxyl" structure. During printing, the ink undergoes initial shaping through extrusion combined with ultraviolet light irradiation. Subsequently, acid-catalyzed deep polymerization of NVP and microcrystallization of PVA are simultaneously triggered in a heated oily support bath, completing photocuring and heat treatment enhancement in one step. This invention utilizes the unique molecular structure of malic acid to construct a highly efficient photo-acid synergistic enhancement mechanism, significantly improving monomer conversion rate and greatly enhancing the mechanical strength and shape fidelity of the printed body through a physical-chemical dual network. This effectively resolves the contradiction between printability and strength in bio-inks, showing broad application prospects in tissue engineering, flexible electronics, and other fields.

The invention discloses photo-thermal controlled-release injectable hydrogel for promoting wound healing through nerve and blood vessel synergistic regeneration as well as a preparation method and application of the photo-thermal controlled-release injectable hydrogel. The preparation method comprises the following steps: preparing an ovalbumin electrostatic spinning fiber membrane; the preparation method comprises the following steps: modifying an ovalbumin electrostatic spinning fiber membrane with dopamine, grafting deferoxamine, and shearing and grinding to obtain the drug-loaded short fiber capable of providing a topological structure to guide cell growth; the preparation method comprises the following steps: mixing drug-loaded short fibers with a multi-walled carbon nanotube dispersion liquid, preparing micro-droplets through a microfluidic device, and carrying out cross-linking curing to obtain photothermal responsive drug-loaded short fiber microspheres; the preparation method comprises the following steps: blending an ovalbumin hydrogel precursor solution with photo-thermal responsive drug-loaded short fiber microspheres, and carrying out catalytic molding through a cross-linking agent under a heating condition. According to the invention, the photo-thermal response microspheres are combined with the hydrogel, so that the regeneration microenvironment is synergistically regulated and controlled by multiple factors, and a new thought is provided for tissue engineering nerve grafts. The method has the characteristics of no toxicity, harmlessness, environmental protection, simplicity, feasibility and easiness in operation.

The invention provides a regenerated silk fibroin-collagen-polydeoxyribonucleotide composite membrane material for guiding bone regeneration as well as a preparation method and application thereof, and the membrane material is prepared from the following raw materials in parts by weight: 6-10 parts of regenerated silk fibroin, 2-4 parts of collagen and 0.5-3 parts of polydeoxyribonucleotide. The regenerated silk fibroin-collagen-polydeoxyribonucleotide composite membrane material prepared by the invention has the advantages of excellent biocompatibility, acceleration of cell proliferation and support of growth of cells on the surface of the regenerated silk fibroin-collagen-polydeoxyribonucleotide composite membrane material. Besides, when the membrane material is used as a tissue engineering and regenerative medical material through a groove mold, the membrane material not only has the advantages of outstanding mechanical performance and good plasticity, but also can induce osteoblast and vascular regeneration of tissues and prolong the maintenance time, is an innovative tissue engineering and regenerative medical material, and has good clinical application value.

The present application relates to a kind of porous scaffold for repairing bone defect loaded with black phosphorus active oxygen elimination and slow-release drug and its preparation method;The scaffold includes the amino cation polymer grafted with the phenylboronic acid (PBA) and cyclodextrin (CD) that can eliminate active oxygen, black phosphorus nanosheet (BP) and scaffold material poly (lactic acid-glycolic acid) (PLGA).The porous scaffold loads a large number of black phosphorus nanosheet that can be hydrolyzed into bone repair nutrients, and the porous structure provides sufficient space for cell migration and growth, realizes the good repair of bone.Active oxygen elimination ability, can eliminate active oxygen in diabetic microenvironment.In addition, cyclodextrin can load a variety of drugs, such as osteogenic differentiation induction drug, and realize the slow release of drug.The porous scaffold has good biocompatibility, and the degradation product is harmless to human body, can be used for the tissue repair of bone defect, can be widely applied in the field of biological medicine and tissue engineering, and has been proved to have good repair ability in the diabetic rat skull defect model.

This invention relates to the field of electrospun membranes, and more specifically to a one-step photocurable electrospun membrane material and its preparation method. The photocurable electrospun membrane material is prepared by mixing 5-20 parts of fiber-forming macromolecules, 10-40 parts of collagen, 0.05-3 parts of natural photosensitizer, and a solvent to form a spinning solution. Electrospinning is performed using either needle-type or non-needle-type methods. Simultaneously, the fibers are irradiated with 200-400 nm ultraviolet light in situ to achieve photocuring and cross-linking, thus obtaining a micro / nanofiber membrane in one step. The fiber-forming macromolecules are selected from biodegradable medical polymers, and the photosensitizers are biosafe components such as riboflavin and retinaldehyde. This invention completely solves the problems of uneven cross-linking, fiber morphology damage, collagen denaturation, and internal light filtering effects in traditional two-step methods. The resulting membrane has regular fiber morphology, uniform cross-linking, high mechanical strength, good water resistance, and complete retention of bioactivity. Furthermore, the process is simplified, complies with pharmaceutical quality management standards, and is suitable for medical applications such as tissue engineering and wound repair.

The invention provides a dental pulp stem cell exosome-loaded acellular tissue engineering nerve conduit as well as a preparation method and application thereof, relates to the technical field of biomedical engineering and nerve regeneration medicine, and aims to solve the problems of poor mechanical property, low bionic degree, high degradation rate, insufficient biological activity and the like of an existing nerve conduit. The technical key point of the invention is as follows: the dental pulp stem cell exosome-loaded acellular tissue engineering nerve conduit is provided, and the nerve conduit is obtained by loading a nanofiber conduit with enhanced function of over-expressing let-7i-5p, the porous nanofiber catheter is a porous catheter which is obtained after a polyvinyl alcohol (PVA)-chitosan (CS)-graphene (G) electrostatic spinning membrane is subjected to pipe reeling and crosslinking, and the nucleotide sequence of let-7i-5p in DPSC-Exo is shown as SEQ NO.1. The invention further discloses a preparation method of the porous nanofiber catheter.

The invention discloses a controllable multi-channel optomagnetic addressing guide system and a method thereof, and belongs to the technical field of precise control and advanced manufacturing. The system comprises a matrix, and a magnetic function unit, a light control unit, a magnetic field control unit and a main control unit which are distributed in the matrix. The main control unit scans one or more light tracks in a substrate by controlling the light control unit, cooperates with a magnetic field generated by the magnetic field control unit, and realizes parallel guidance and addressing control of the multiple groups of magnetic functional units by utilizing the combined action of a photothermal effect and the magnetic field. By changing parameters such as laser wavelength, the system can selectively activate and sort specific functional units. The method overcomes the defect that multiple targets cannot be independently, parallelly and accurately guided in the prior art, has strong addressing capability, high parallelism and flexible controllability, and can be widely applied to the fields of targeted drug delivery, microcosmic assembly, tissue engineering, reconfigurable flexible electronics and the like.

The present disclosure pertains to universal and adoptable cell culture substrates designed for applications in biology, medicine, pharmaceutics, and other relevant fields. The disclosure particularly focuses on processes and methodologies for manufacturing such substrates, which prove advantageous for both non-neural (cells exhibiting robust adhesion and weak cell-cell junctions, as well as cells with regular adhesion and cell-cell junctions) and neural cell sheet engineering. These substrates aim to enhance cell adhesion, stimulate cell growth, and enable rapid and uniform harvesting of cell sheets. Additionally, the present invention introduces a scaffold-free method for tissue engineering, facilitating the creation of a 2D / 3D neural tissue construct featuring unidirectional neuron bundles.

The Cardiac Tissue Engineered Model (TEEM) invention provides a robust in vitro model for cardiotoxicity evaluation using three-dimensional (3D) human heart microtissues to quantify dose-dependent changes in electromechanical activity, resulting in a comprehensive cardiotoxicity and arrhythmia risk assessment of test compounds. The invention also provides a predictive in vitro screening platform for pro-arrhythmic toxicity testing using human three-dimensional cardiac microtissues. The invention enables the screening of environmental and pharmaceutical compounds, chemicals, and toxicants to establish safe human exposure levels.

The invention discloses an amino acid sequence of adipose-derived stem cell affinity polypeptide, a screening method and application of the adipose-derived stem cell affinity polypeptide. The amino acid sequence of the adipose-derived stem cell affinity polypeptide provided by the invention is KMISTIS. In the invention, the inventor screens out the affinity peptide aiming at the human ADSC by utilizing a phage display technology, and aims to improve the implantation efficiency of the ADSC in tissue engineering. The peptide is capable of binding to the surface of an acellular fat matrix (DAM), thereby recruiting endogenous ADSC.

The application discloses a kind of microfiber scaffolds, organoid tissue patches and preparation method and application thereof.The microfiber scaffold is prepared using melt electrospinning process, by introducing stepwise translation deposition path in the same deposition plane, so that the continuous deposition of multiple microfibers forms stable convergence node structure at predetermined position, thereby constructing microfiber network with regular pore and continuous support topology.The stepwise translation deposition includes at least one repeated deposition with preset in-plane offset after the completion of the first pass fiber deposition, to reduce the electrostatic interference between fibers and promote node formation.The scaffold structure is stable, open and thin, suitable as a tissue engineering support structure.The application also provides an organoid tissue patch composed of the scaffold loaded organoids and a construction method thereof, which can be used to cover tissue injury interface, such as post-hepatectomy section repair.

The invention discloses a kidney-related tissue engineered cell in-vitro culture chip, and relates to the field of cell in-vitro culture, the kidney-related tissue engineered cell in-vitro culture chip comprises a biochip and a glass slide, the biochip is made of PDMS (polydimethylsiloxane), the lower surface of the biochip and the glass slide are bonded to form a closed culture structure, one side of the biochip demolded by a photoetching template is provided with a fixing groove, and the other side of the biochip is provided with a clamping groove. Flow channels are formed in the edge parts of the two sides of the inner wall of the fixed groove, and a plurality of permeation columns are arranged in the fixed groove; the cell adding holes in different areas of the chip can be used for loading different types of tissue-engineered cells, the permeation columns enable culture areas to be isolated, liquid can permeate into the internal areas from outside to inside, transmission of different cell factors and interaction between cells are achieved, different chips do not need to be connected in series, and the cost is low. The problems of liquid leakage of series pipelines and mixing of different types of tissue engineered cells in the prior art are thoroughly solved, and compared with the prior art, the co-culture stability is improved.