37 results about "Native tissue" patented technology

The invention provides a preparation method of a cell culture three-dimensional scaffold and cell culture meat, which comprises the following steps: firstly, preparing a gelatin-carrageenan mixed solution and a gel precursor solution of transglutaminase, dividing into two parts, incubating at constant temperature to obtain two parts of composite hydrogel, randomly freeze-drying one part to obtain aerogel, and freeze-drying the aerogel to obtain the cell culture three-dimensional scaffold. Stacking on the other part and incubating at constant temperature again to obtain the prefabricated double-layer gel. The prefabricated double-layer gel is directionally frozen, ice crystals grow upwards from the bottom, freezing is stopped after the temperature is reduced to the set temperature, and the cell culture three-dimensional scaffold is obtained through drying. The stent adopts an ordered-disordered partition design, parallel micro-channels are formed in the lower layer by using a directional ice template method, and muscle fiber arrangement is simulated; a porous spongy structure is constructed on the upper layer by using a random ice template method, and is adapted to adipocyte proliferation. Two layers of interfaces are interpenetrated and communicated, natural mechanical gradients are combined, natural tissue characteristics are re-engraved, the traditional limitation is broken through, the cell co-culture efficiency and the cultured meat quality are improved, and industrialization is promoted.

Provided is a use of a biological material in preparing a product for limiting gastric volume. The biological material is selected from a decellularized matrix, an artificial extracellular matrix and a high molecule compound. The wrapping of a remaining stomach after gastric volume reduction surgery by the biological material can effectively reduce the expansion of the gastric volume after the surgery for a long time. The gastric volume of the remaining stomach can be effectively maintained, avoiding the occurrence of weight regain caused by the expansion of the remaining stomach. The decellularized matrix and a biological material remolded tissue have the viscoelasticity equivalent to or close to natural tissue, avoiding gastric wall stiffness, and thus not affecting gastric wall peristalsis.

The invention relates to the technical field of biological scaffolds, in particular to a silk fibroin-based biological 3D intelligent response scaffold and a preparation method and application thereof.The silk fibroin-based biological 3D intelligent response scaffold adopts a three-layer bionic gradient structure of an oriented fiber membrane, a porous sponge layer and an interface layer, simulates natural tissues, and has the advantages that the bionic gradient structure is improved; mechanical guidance, cell inhabitation and biological activity interfaces are provided respectively, and high-quality regeneration of blood vessels, nerves and skin is promoted synergistically. According to the present invention, the pH / enzyme / temperature triple response unit is provided, such that the lesion microenvironment can be perceived, the targeted release can be triggered, the problem of high burst release rate of the traditional physical drug loading can be solved, and the on-demand accurate drug delivery can be achieved. The SF / HPMC gel is adopted for 3D printing, the printing precision is high, and the porosity difference is 1t; the product performance is stable, and the pore structure can be precisely designed and customized according to the regeneration requirements of different tissues (such as blood vessels, nerves and skin).

A valve device comprises a valve body with a framework and a valve portion coupled to the framework that is movable between open and closed positions. The framework comprises a plurality of wire portions. The valve portion may be formed of a material that promotes in-growth of native tissue so that the valve portion cooperates with native valve leaflets. The valve device also comprises one or more anchors and anchor tethers that secure the valve device in a heart. In some examples, the method includes folding and loading the valve body into a valve cartridge, delivering the valve body through a guide tube to a position the valve body at a target positioned within the heart, pushing the valve body out of the guide tube, and secure the anchors and anchor tethers in the heart, and cutting the anchor tethers to remove excess portions.

The LNTPI is a surgical instrument used to cut through fibrotic and edematous tissue to define the natural tissue plane of dissection. After defining the natural tissue plane, it is designed to move or carefully insinuate tissues along these natural planes without cutting the tissue, so that targeted organs or parts thereof could be viewed and reached during laparoscopic procedures. The following features make the LNTPI unique compared to standard blunt dissectors used in laparoscopy: 1. The tip of the LNTPI is coarse and not smooth. The tip has diagonal, spiral ridges from the sides which are carried onto the tip converging as a single spiral at the center of the tip, creating a pattern resembling that of a screw; 2. The ridges of the LNTPI are comprised of asymmetrical triangular ridges with an acute angle at the apex that may be leaning along with or against the rotation of the spiral; 3. The tip of the LNTPI is made of tungsten carbide metal instead of stainless steel; 4. Dissection can be done in the usual up, down, left, and right motions as standard dissectors; 5. The side of the LNTPI can be used to "peel" tissue by using the ridges on its side combined with a twisting movement. This maneuver cannot be performed using standard dissectors; and 6. The tip of the LNTPI can perform circumferential dissection when used with a twisting movement causing it to engage tissue like a "drill" along the natural tissue plane. The ridges that converge into a single spiral at the tip of the invention appear like a screw and with the proper twisting movement have a similar effect on tissue. Because of the texture, design, and material used for the tip of the LNTPI, it has the following advantages over standard dissectors: The LNTPI can engage tissues more easily even if the tissues are slippery due to the presence of blood or fluids. The LNTPI can be used to engage tissues through a twisting motion which motion allows the tissues to be engaged in a specific manner that defines natural tissue dissection planes. The LNTPI can insinuate / "peel" tissue by using the side spiral ridges in combination with a twisting movement. The LNTPI can perform circumferential dissection by using the tip and the convergence of the spiral ridges at the center of the tip in combination with a twisting movement. The transfer of lateral shear force to the tissue at various angles (45, 90, and 180 degrees) when using the LNTPI is significantly higher whether the motion is by a sweeping motion or twisting of the instrument, allowing it to insinuate better than standard instruments, especially in a wet field. Hand movements needed in insinuating tissue using the LNTPI are different from the usual hand movements when using standard dissection instruments. The hand movements in using LNTPI require less muscular effort and repetitiveness, thus preventing muscle fatigue and repetitive use injury among surgeons. The instrument is reusable, as compared to dissectors that have fabric at the tip, which must be disposed of and replaced once the fabric tip fully absorbs the blood or other fluid in the wet tissue surface.

Bone plates are disclosed for veterinary uses. For example, the bone plates could be utilized for repairing medial patellar luxation in animals, such as canines, felines, or equines, for example. The bone plates may include various features for better accommodating the anatomy and native tissue structure of the proximal tibia of the animal.

Particulated and reconstituted tissues comprising small, densely packed tissue microparticles encapsulated in a tissue specific promoting gel packed at a percolation threshold that can be transplanted into damaged tissue thereby facilitating regeneration following trauma to the tissue. The engineered microparticle construct for tissue replacement and repair, as taught herein, provides numerous benefits including (1) encouraging a regenerative response in damaged tissue regions, (2) mimicking the structural support of native tissue, (3) establishing an environment that promotes attachment, migration, and differentiation of infiltrating stem cells, and (4) providing a source of growth factors and other anti-catabolic growth factors and cytokines. Tissue specific microparticles packed together at, or past, their percolation threshold will provide the necessary mechanical environment and to best recapitulate and integrate with native tissue. The packing of microparticles, derived from the ECM of native tissue to a concentration past the percolation point will yield both the necessary biochemical and biomechanical properties necessary for reconstituting a specific tissue.

Devices, systems, and methods for preventing leakage through a therapeutic implant are provided. An exemplary apparatus includes an elongate wire configured to be implanted within a heart of a patient such that the elongate wire is positioned within a non-circular gap between a therapeutic implant positioned within the heart and a native heart tissue of the patient to stop a leakage of blood through the non-circular gap. The elongate wire comprises a single length of material set in a serpentine shape. Moreover, the elongate wire is configured to retain the serpentine shape when the elongate wire is positioned within the non-circular gap.

The invention generally relates to mass spectral analysis. In certain embodiments, methods of the invention involve analyzing a lipid containing sample using a mass spectrometry technique, in which the technique utilizes a liquid phase that does not destroy native tissue morphology during analysis.

A medical implant is provided defined as an electrospun hollow-cored porous suture ring. The suture ring is a continuous ring made by rolling up circular sheet of electrospun material, which was electrospun over a cylindrical target. The suture ring, upon implantation, is capable of being absorbed and replaced by natural tissue due to ingrowth of cells and nutrients into pores of the electrospun hollow-cored biodegradable suture ring. The suture ring addresses at least some of the existing problems with suture or sewing rings.

The invention discloses a penis prosthesis cylinder based on a function separation and photocuring technology and an individualized shaping method thereof. The core lies in that a'function separation 'design normal form is adopted, an external individualized shaping sleeve and internal fluid pressure are utilized to realize intraoperative temporary shaping, and thiol-ene and other efficient photocuring materials are subjected to irreversible cross-linking reaction under illumination to realize permanent locking of forms. The cylinder prepared by the method has an individualized shape which is highly matched with a cavernous body cavity of a patient, and has softness (hardness of 25A-30A) close to that of natural tissues and excellent anti-fatigue performance (gt; and 500,000 times of circulation). More importantly, the conversion rate of the selected material system is high (gt; and after curing, the product does not need to be subjected to complicated post-treatment, and can be implanted only by flushing with normal saline. The cylinder can be integrated with a general pump valve and a liquid storage bag through a standard connector to form a complete three-piece prosthesis system, the postoperative displacement rate can be remarkably reduced, the stick feeling is eliminated, and the three-piece prosthesis has great clinical value and wide industrialization prospects.

The invention discloses an artificially implanted heart valve which has an axial direction, an inflow side and an outflow side in space, the artificially implanted heart valve comprises an inner frame, the inner frame is of a barrel-shaped structure which can deform in the radial direction, the inner frame is of a grid structure, and each grid is defined by frame strips; a valve leaflet; the outer frame is of an integrated annular structure and comprises a plurality of arm parts in the circumferential direction, the outflow side of each arm part is fixedly connected to the inner frame, the inflow side of each arm part is located on the periphery of the inner frame, and a first gap capable of clamping native tissue is formed between the inflow side of each arm part and the inner frame; the outflow sides of every two adjacent arm parts are close to each other to form an intersection part, each intersection part is provided with a forked structure extending towards the corresponding outflow side, and the forked structures extend along the frame strips of the inner frame. The intersection part of the artificially implanted heart valve extends to the inner side of the inner frame and is attached to the inner side of the inner frame, so that the artificially implanted heart valve is convenient to load and adjust the posture in the body in the releasing process, and operation is more convenient.

The present technology is directed to adjustable interatrial shunting systems that selectively control blood flow between the left atrium and the right atrium of a patient. The adjustable interatrial devices include a shunting element having an outer surface configured to engage native tissue and an inner surface defining a lumen that enables blood to flow from the left atrium to the right atrium when the system is deployed across the septal wall. The systems can include an actuation assembly for selectively adjusting a geometry of the lumen and / or a geometry of a lumen orifice to control the flow of blood through the lumen.

Systems and methods for additive-manufactured biocompatible implants. Many embodiments are directed to 30-printed biocompatible resins for fabricating custom implants. In several embodiments, the biocompatible resin is based on a ratio of biocompatible polymers. The biocompatible resin, in accordance with various embodiments, incorporates an antibacterial and / or antimicrobial agent, In some embodiments, the biocompatible resin incorporates a bioceramic to mimic native tissue.

Systems and methods for additive-manufactured biocompatible implants. Many embodiments are directed to 30-printed biocompatible resins for fabricating custom implants. In several embodiments, the biocompatible resin is based on a ratio of biocompatible polymers. The biocompatible resin, in accordance with various embodiments, incorporates an antibacterial and / or antimicrobial agent, In some embodiments, the biocompatible resin incorporates a bioceramic to mimic native tissue.

The invention provides an acellular matrix fiber woven scaffold as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. The acellular matrix fiber woven scaffold comprises a woven structure unit, wherein the woven structure unit comprises warp yarns and a plurality of acellular matrix fiber weft yarns; the acellular matrix fiber is prepared by cutting an acellular matrix paper-based material at equal intervals; the plurality of acellular matrix fiber wefts are arranged in parallel according to a bionic trend and are interwoven and fixed through the warps, so that a bionic pattern simulating natural tissue fiber arrangement is formed in the weaving structure unit. The acellular matrix fiber scaffold is prepared by innovatively applying a weaving technology, the scaffold can improve the cell loading efficiency, is beneficial to self-repair and reconstruction of damaged tissues, provides suitable survival and growth conditions for cells, and has good application prospects in the field of biomedical engineering, especially in the aspects of tissue repair and regenerative medicine. And huge application potential is shown.

The invention discloses application of a composite biological material in hernia repair surgery, and belongs to the technical field of biotechnology, the composite biological material comprises a nanofiber scaffold, the scaffold is prepared by compounding polylactic acid-glycolic acid and silk fibroin, the weight ratio of the polylactic acid-glycolic acid to the silk fibroin is 70: 30, and the weight ratio of the polylactic acid-glycolic acid to the silk fibroin is 20: 30. The nanofiber scaffold is prepared through an electrostatic spinning technology and is used for providing mechanical support and promoting cell attachment and proliferation; the fiber is of a porous structure, the pore size is 10-100 microns, and cell permeation and nutrient substance exchange are facilitated; the polylactic acid-glycolic acid / silk fibroin composite scaffold is prepared by compounding polylactic acid-glycolic acid and silk fibroin, the mechanical properties of natural tissues are simulated, and a good growth scaffold is provided for cells. The gel layer is prepared through a solution phase separation method, and provides additional biological activity support and a cell attachment surface for the nanofiber scaffold. By arranging the antibacterial coating, the risk of postoperative infection is reduced, and meanwhile, protection is provided for materials and human tissues.

The application provides a high-strength high-biocompatibility tough hydrogel and a preparation method and application thereof. The preparation method is a method of repeatedly stretching in a sulfate solution and soaking in a PBS buffer solution in cycles, and a high-strength high-biocompatibility tough hydrogel with high order, high crystallinity and anisotropy is prepared. The preparation method is simple and easy to operate, low in cost and high in commercial value; the preparation method is combined with a 3D printing technology to prepare a customized high-strength high-biocompatibility repair bracket. The tough hydrogel prepared by the above method is applied to tissue engineering and regenerative medicine research, can realize mechanical compensation conduction force, and due to obvious toughness and biocompatibility, in-vivo self-degradability and similarity with natural tissue components, is directly sutured to a broken tendon of an adult rabbit, and tendon tissue repair is completed within 8 weeks. In addition, liquid metal can be injected into the tough hydrogel to realize detection of various parts of the human body.

Closure devices that selectively control blood flow between the right atrium and the left atrium of a heart of a patient. The control of blood flow can be based on detected values from a pressure sensor. The closure devices can include a body configured to engage native tissue when the device is deployed across the septal wall. In a first state at least a portion of (an inner surface of) a lumen is closed, preventing blood flow through the closure device. In a second state, the lumen is open to enable blood flow from the left atrium to the right atrium. A closure element can be selectively adjusted to control the flow of blood through the lumen. A volume of an expandable chamber can be increased or decreased to place the closure device into the first state or the second state.

An assembly for the tricuspid orifice of a human heart has an external frame connected to an internal stent carrying a tricuspid valve bioprosthesis and a sealing skirt. The external frame is configured to hold its position in the native tricuspid annulus. The internal stent is connected to the external frame by one or more fixing strands. The sealing skirt covers the interstitial space existing between the external frame and the internal stent. Developments are described which include the use of a deformable region of the frame, various alternative embodiments of the sealing skirt, the use of a frame composed of multiple sub-sections, the use of fixing strands between the stent and the frame and of fixing elements for fixing the assembly to the native tissue, the use of sensors and / or actuators, and also the use of a stent in the inferior vena cava. Method aspects are also described.

The invention discloses a three-layer artificial soft tissue repair membrane with progressively-changed porosity in the field of biomedical tissue engineering, and the repair membrane sequentially comprises an ordered fiber layer, a spiral disordered fiber layer and an ordered fiber layer from inside to outside to form a structure with progressively-increased porosity. Each layer is made of biodegradable materials which can be selected from various natural or synthetic polymers. The diameter of the fiber is in a range from nanoscale to micron scale, and the distance between the fibers of the ordered fiber layer is adjustable and is gradually increased from inside to outside. The membrane can load various bioactive substances and drugs, and the surface of the membrane can also be modified by growth factors or nano materials and the like. The preparation method mainly adopts a near-field direct-writing 3D printing process and is realized by regulating and controlling printing parameters. The obtained repair membrane is unique in pore structure, can simulate natural tissues and promote cell ordered growth and tissue repair, and is suitable for the field of tissue engineering scaffolds.

The invention discloses an artificially implanted heart valve, which comprises an inner frame, an outer frame, an outer frame, an inner support, an outer support, an outer support and an inner support, and the inner frame is of a barrel-shaped structure which can deform in the radial direction; a valve leaflet; the outer frame is of an integrated annular structure and comprises a plurality of arm parts in the circumferential direction, the outflow side of each arm part is fixedly connected to the inner frame, the inflow side of each arm part is located on the periphery of the inner frame, and a first gap capable of clamping native tissue is formed between the inflow side of each arm part and the inner frame; the arm part sequentially comprises a root part, a middle part and a head part from the outflow side to the inflow side, in a release state, the circumferential span of the arm part is gradually narrowed from the root part to the head part, an isolation gap is formed between the middle part and the periphery of the inner frame, the head part is wrapped with a protective film, and the protective film is fixed to the head part in a wire binding mode. The intersection part of the artificially implanted heart valve extends to the inner side of the inner frame and is attached to the inner side of the inner frame, so that the artificially implanted heart valve is convenient to load and adjust the posture in the body in the releasing process, and operation is more convenient.

A tissue dissection system for directing a cannula between natural tissue layers includes a tube defining a lumen therethrough; an attachment and stabilization device configured to receive and guide the tube at an operation angle with respect to the natural tissue layers; and an actively steerable cannula (ASC) configured to pass through the lumen of the tube between the natural tissue planes. The ASC defines first and second substantially parallel lumens therethrough configured to receive corresponding first and second steering wires. An attachment and stabilization device for a tissue dissection system is configured to receive and guide a tube at an operation angle with respect to natural tissue layers. The device includes a base having a size and shape so as to securely attach to a body part of interest for tissue dissection.

The present invention relates to a semi-finished gel and a process for producing such semi-finished gel, suitable for use in the preparation of food gel casings, derived from a first natural tissue and a second natural tissue. The present invention further relates to a method for producing a homogeneous collagen-elastin gel, comprising a de-entanglement process in an extrusion system having a de-entanglement device including an interlocking member. The extrusion system further has a food dough extrusion passage and two cooperating jackets arranged coaxially with the food dough extrusion passage, at least one of which is rotatable around a co-extrusion axis, the jackets define an annular band between them having an annular band inlet for introducing the gel, the rotation of at least one of the jackets causes the gel to be oriented, thereby converting the introduced gel into an oriented gel, the annular band has opposing annular band outlets for discharging the oriented gel; the discharged oriented gel is deposited on the food dough coming out of the food dough outlet to form a food having a gel casing.

The invention relates to a human recombinant collagen based on cell expression self-assembly, which is closer to a triple helix structure of natural collagen, and successfully realizes efficient expression and correct folding of recombinant III type collagen through a genetic engineering cell expression system and rational amino acid sequence design. Through verification, the obtained product can be spontaneously assembled to form a stable natural triple-helix structure, and the optimized molecular conformation enables the product to have the following remarkable advantages: the product is closer to the mechanical property of a natural tissue; the biological activity is improved; the cell affinity is obviously improved.

Described herein are methods for preserving biomaterials by vitrification while reducing or preventing the loss of viability associated with conventional preservation methods. Also described herein are cassettes and methods for using these cassettes for cryopreserving biomaterials (e.g., a bioengineered construct or natural tissue sample).

Devices, systems, and methods for preventing leakage through a therapeutic implant are provided. The apparatus includes an implantable clip having a single length of wire. The single length of wire is shaped into a first arm, a second arm, and a loop positioned laterally between the first arm and the second arm and longitudinally proximal of the first arm and the second arm. The first arm is configured to be inserted into a therapeutic implant within a heart of a patient and the second arm is configured to be inserted into a native tissue of the heart. The first arm and the second arm are configured to urge a border of the therapeutic implant and a border of the native tissue toward one another and close a gap between the therapeutic implant and the native tissue.

This application belongs to the field of cell biology and tissue engineering technology, specifically relating to a method and composition for in vitro regeneration of meibomian gland organoids. This application identifies Prom1⁺ cells, bipotent progenitor cells capable of maintaining meibomian gland homeostasis, at the acinar-duct junction. This application establishes a three-stage organoid culture system that enables the expansion and culture of meibomian gland-derived somatic cells containing Prom1⁺ cells. By precisely controlling signal transduction at specific stages, the developmental trajectory of meibomian gland tissue is accurately reproduced, generating functional organoids. These organoids exhibit high transcriptional fidelity to natural tissues and significantly retain the natural lipid profile. The method of this application is applicable to culturing starting cell populations containing Prom1⁺ cells to obtain functional meibomian gland organoids, providing a high-fidelity model for studying meibomian gland biology, simulating meibomian gland degeneration (MGD), and developing cell-based DED therapies.

According to some embodiments, a method of inserting a lateral implant within an intervertebral space defined between an upper vertebral member and a lower vertebral member includes creating a lateral passage through a subject in order to provide minimally invasive access to the intervertebral space, at least partially clearing out native tissue of the subject within and / or near the intervertebral space, positioning a base plate within the intervertebral space, wherein the base plate comprise an upper base plate and a lower base plate and advancing an implant between the upper base plate and the lower base plate so that the implant is urged into the intervertebral space and the upper vertebral member is distracted relative to the lower vertebral member.

The invention belongs to the technical field of bioengineering, and relates to a cartilage organ regulation and control scaffold and application thereof, the cartilage organ regulation and control scaffold comprises a top layer structure and a bottom layer structure; the top layer structure is obtained by gelatinizing a first solution; the bottom layer structure is obtained by gelatinizing a second solution; the first solution and the second solution are different in composition; the preparation raw materials of the first solution and the second solution independently comprise collagen, growth factors, tissue development related proteins, proteoglycan and a solvent. The different compositions of the solutions result in their unique physical properties that affect the organoid physiological activity by delivering biophysical signals to the organoid. By means of the unique two-phase structural design, mechanical properties such as gelation degrees of the bottom layer and the top layer are accurately controlled, three-dimensional cell aggregates can be easily matched in the stent, enough three-dimensional elastic space is reserved for subsequent stable peripheral expansion of the stent, better mechanical stability is provided, and the stent is close to natural tissue in shape and function.