127 results about "Autologous tissue" patented technology

This invention provides implants comprising tissue having an intercellular matrix anchored to a biocompatible scaffold. The intercellular matrix of the tissue provides a natural medium to facilitate the healing and growth of damaged tissue in a patient. The present invention provides methods of treating damaged tissue in a patient by inserting such implants into the damaged tissue. The implants of the present invention include implants comprising allogenic and / or autologous tissue. The tissue may also be acellular.

The present invention relates to an in vivo method of promoting the growth of autologous tissue and its use to form corrective structures, including tissue that can be explanted to other locations in the animal. In particular, the invention relates to methods and systems for (a) the site-specific regeneration of tissue, and (b) the synthesis of neotissue for transplantation.

The present disclosure relates to an implant for insertion into a tissue defect, such as a cartilage defect or a cartilage and bone defect. The implant includes a plug including a porous polymeric material having at least one channel therein, wherein the plug is sized to fit the tissue defect; and tissue. The tissue substantially fills the channel and is selected from a group including autologous tissue, allogenic tissue, cultured tissue, or combinations thereof. In an embodiment, the plug includes a plurality of porous polymeric phases. In another embodiment, the plug includes a plurality of channels wherein the channels are longitudinal and / or transverse. A method for repairing defective tissue is also disclosed.

A method of carrying out an autologous tissue implant in a subject in need thereof is carried out by: (a) forming an autologous tissue implant from autologous cells collected from a subject (e.g., by ink-jet printing, the autologous cells and the scaffold, separately or together), and then (b) implanting the autologous tissue implant in said subject.

An implantable tissue grafting medical system and material using a combination of bio-absorbable and non bio-absorbable fibers and materials such as Poly Glycolic Acid (PGA) and polyester (PET), provides a permeable mesh or weave of fibers with an initial interstice size and permeability factor suitable to initial implant requirements, and a pre-engineered bio-absorption pattern and rate that controls the gradual expansion of interstice size within the mesh or weave in one or two dimensions up to a pre-engineered maximum interstice size, consistent with the anticipated rate of tissue regeneration on the implant, while retaining a primary grid or circumferential pattern of non-absorbable fibers at the maximum interstice size for supporting the new tissue for an extended period. Various means for combining materials to obtain initial interstice size, pattern and permeability, with the desired absorption pattern and rate, and the desired end point interstice size and spacing, are also disclosed.

An implantable prosthesis for percutaneous placement within a vein that forces opposing portions of the vessel wall of a vein together to create a new valve of autologous vein tissue to be operable to alternate between a valve closed configuration and a valve open configuration. When in a preset closed configuration, the implantable prosthesis pushes or pulls portions of the vessel wall of the vein together to substantially close the vein lumen and prevent retrograde blood flow from backflowing through the new valve in the valve closed configuration. The implantable prosthesis has leg portions that may be pushed apart in response to antegrade blood flow through the vein to allow the new valve to achieve the valve open configuration.

Methods are described for generating autologous tissue grafts, including generating grafts at the point of case, which include isolated cell populations that are enriched with stem cells and are mixed with hyaluronic acid and derivatives thereof. The hyaluronic acid localizes the cells to a desired injection site and stimulates collagen production thus enhancing the viability and the longevity of the graft.

Apparatus and methods are described for generating autologous tissue grafts, the apparatus including a point of care SVF isolation unit that includes a tissue digestion chamber in fluid communication with a lipid separating chamber, whereby SVF cells are isolated without centrifugation; and a cell seeding chamber in fluid communication with the SVF isolation unit, said cell seeding chamber adapted to support a cell scaffold. Methods and materials for cell seeding, including through the provision of micro rough scaffold surfaces, are also provided.

Percutaneous methods of forming a venous valve from autologous tissue are disclosed. The methods include percutaneously creating one or two subintimal dissections for forming one or two flaps of intimal tissue. In one method, a puncture element is delivered by a catheter based delivery system to a treatment site where a new venous valve is to be created. The puncture element is deployed to gain access to a subintimal layer of the vein wall. A dilation balloon is than positioned and inflated within the subintimal layer to create a flap and corresponding pocket / sinus in the vein, which than acts as a one-way monocuspid valve in the manner of a native venous valve. In a similar manner, methods of forming new bicuspid venous valves by subintimal dissections are also disclosed.

Percutaneous apparatus for forming a bicuspid venous valve from autologous tissue are disclosed. A multilumen catheter is disclosed that includes a delivery shaft positioned on either side of the balloon. When the balloon is inflated within the vein at a treatment location where a bicuspid valve is to be created, the delivery shafts are pressed into the wall of the vein by the inflated balloon so that exit ports in the delivery shafts are at diametrically opposed locations. The delivery shafts may than be used to deliver puncture elements through the exit ports and into the vessel wall to gain access to a subintimal layer of the vein wall. In this manner, the inventive multilumen catheter aids in making properly positioned flaps of venous tissue for creating a bicuspid venous valve from autologous tissue.

The invention relates to a preparation method of a tissue repair material. The preparation method comprises the following steps: carrying out pre-treatment; vibrating in an organic solvent to degrease; deactivating a virus with an ethanol solution; removing cells by high and low osmotic solutions; swelling in an acidic or alkaline liquid; and quickly lyophilizing and sterilizing. The thickness of the tissue repair material is increased by 2-4 times, and the material is loose and porous, small in change of mechanical property and degradation property and good in cell removal effect and has relatively high biosafety and biocompatibility. Due to the structural characteristics of proper thickness and looseness and porosity, the material is suitable for damage repair for filling damaged parts required by repair such as cartilage injury, skin and soft tissue defect, gingival recession and the like. Animal experiments verify that the repaired region after eight weeks has no depressed phenomena and is fully replaced by regenerated tissues. Moreover, the regenerated tissue and autologous tissue are healed well and are free of separating phenomenon, which verifies that the material can provide sufficient three-dimensional space for cell proliferation and crawl, so that the repaired region has good integration degree and filling degree, thereby realizing tissue repair and regeneration.

Percutaneous methods of forming a venous valve from autologous tissue are disclosed. The methods include percutaneously creating one or two subintimal dissections for forming one or two flaps of intimal tissue. In one method, a puncture element is delivered by a catheter based delivery system to a treatment site where a new venous valve is to be created. The puncture element is deployed to gain access to a subintimal layer of the vein wall. A dilation balloon is than positioned and inflated within the subintimal layer to create a flap and corresponding pocket / sinus in the vein, which than acts as a one-way monocuspid valve in the manner of a native venous valve. In a similar manner, methods of forming new bicuspid venous valves by subintimal dissections are also disclosed.

Percutaneous methods and apparatus for forming a venous valve from autologous tissue by creating at least one subintimal longitudinal dissection that forms at least one flap of intimal tissue are disclosed. In one method, a balloon catheter having a dissecting blade mounted thereon is delivered to a target site where a new venous valve is to be created. The balloon is inflated to deploy the blade against the vein wall, and the catheter is longitudinally translated such that the blade dissects a subintimal layer of the vein wall. The balloon is subsequently deflated such that the blade pulls a flap of the dissected tissue towards the vein lumen, thereby creating a leaflet and corresponding pocket / sinus in the vein that collectively act as a monocuspid venous valve. Methods of forming new bicuspid and tricuspid venous valves utilizing two or three dissecting blades mounted on the balloon are also disclosed.

Percutaneous methods and apparatuses for forming a venous valve from autologous tissue. A catheter having a retractable dissecting system received therein is delivered to a target location where a new venous valve is to be created. A distal balloon or other radially-expandable component mounted on the catheter is expanded against the vein wall, and the dissecting system is proximally retracted to deploy one or more dissecting components that dissect a subintimal layer of the vein wall. Radial expansion of the dissecting component(s) within the vein wall creates one or more leaflets and corresponding pocket / sinuses in the vein that collectively act as a venous valve, and / or the radially-expandable component of the catheter is subsequently collapsed such that the dissecting component(s) each pull a flap of the dissected tissue towards the vein lumen to create one or more leaflet(s) and corresponding pocket / sinuses in the vein.

The invention relates to a biological material for repairing meniscus tear and a preparation method for the biological material. The material consists of an acellular matrix membrane on the inner layer, a porous structure collagen matrix membrane on the middle layer, a cell layer formed by cartilage cells on the outer layer and a cell sheet. The repair material has certain elasticity and toughness and is high in biological activity; the cartilage cells can secrete cell factors per se, and nutritional ingredients can be directly obtained through articular cavity synovia, and thus, the establishment of communication link between tissue cells at both ends of the tear during the meniscus tear process is accelerated; the immigration and the infusion of the cells in the material and autologous tissue cells are promoted; the tissue regeneration and functional reconstruction time is shortened; and the ideal novel material and the preparation method for the material are provided for clinically repairing the damage of the meniscus tear (including blood supply zones), especially the tissue regeneration and the functional reconstruction of no-blood zones and low-blood zones.

The invention discloses a material increase manufacturing method of a multi-scale biomimetic artificial bone support. The material increase manufacturing method comprises blending a biopolymer material and a bioceramic material, blending the mixture and deionized water or an organic solvent to obtain uniform slurry, carrying out freeze drying to obtain uniform powder, blending the uniform powder and deionized water or an organic solvent, carrying out vacuum exhaust, carrying out quantitative extrusion by a screw pump, by a XYZ motion device, designing internal aperture shapes, sizes and porosity of a geometric model by computer program according to a bone defect 3D geometric model obtained by CT scanning, and carrying out material increase manufacture under control of an extruded material motion locus. Through use of a degradable biological compound material, human autologous tissue finally replaces bone defect positions so that human lifelong growth requirements are satisfied. The needed biomaterial is quantificationally extruded by the screw pump so that use amount can be accurately controlled and an artificial bone clinical application cost is substantially reduced. The material increase manufacturing method has the advantages of accurate printing material use amount, wide material application range, accurate and controllable aperture structure, and free formation of a macroscopic geometric shape.

Tissue repair in-vivo depends on acute inflammation, but in many clinical situations the other major components of healing such as blood supply, anabolic hormones, growth factors, and stem cells are lacking. This invention includes compositions consisting of an agent which induces an inflammatory healing response combined with an autologous platelet lysate at a specific concentration which may have demonstrated in-vitro abilities to expand autologous tissue repair cells.

A method, apparatus and kit for preparing and performing a surgical procedure includes providing a medicinal composition having an autologous tissue medium and a therapeutic agent. the medicinal composition is then delivered to a surgical site in a patient before closing to reduce postoperative pain, provide an interpositional membrane to prevent or inhibit the formation of scar tissue, and / or promote hemostasis. The medicinal composition is suitable for use in a variety of types of surgery, and has particular application in bone or tissue grating and orthopaedic surgery, expecially spine surgery.

The invention relates to a heart valve implantation device provided with an anchoring device. The device comprises a heart valve prosthesis, at least two sets of anchoring pins and an anchoring pin releasing device, wherein an anti-disengagement end is arranged at the near end of each anchoring pin, the anchoring pin releasing device is detachably connected with the heart valve prosthesis and comprises a delivery pipe and a push rod, the far end of the anchoring pin releasing device is in a preset shape to enable the far end of the delivery pipe to be bent integrally or partially, the part, capable of being bent, of the delivery pipe is the bent section, a rigid section is arranged at the farthest end of the delivery pipe, the distance between the farthest end of the delivery pipe and the nearest end of the bent section of the delivery pipe is larger than or equal to one sixth of the perimeter of a circle formed by means of the minimum radius of the autologous valve tissue ring of a patient, the anchoring pins are pre-arranged in the far end of the delivery pipe and located at the far end of the push rod, and the anchoring pins can be made to move towards the far end of the delivery pipe by pushing the push rod to enable the heart valve prosthesis to be fixed between the autologous tissue and the anti-disengagement end. By the adoption of the device, accurate positioning and firm anchoring can be realized.

The invention relates to a novel cardiac valve implantation instrument with an anchoring device. The novel cardiac valve implantation instrument comprises a cardiac valve prosthesis, at least two sets of anchoring needles and at least two sets of anchoring needle releasing devices detachably connected with the cardiac valve prosthesis. The far ends of the anchoring needles are in preset shapes, the anchoring needle releasing devices comprise conveying pipes, push rods and hollow guide rails, the guide rails are arranged in the conveying pipes in advance and can move towards the far ends of the conveying pipes, rigid sections are arranged at the far ends of the guide rails, the anchoring needles are arranged in the guide rails in advance in an extension state, the push rods are arranged in the conveying pipes and located at the near ends of the anchoring needles, the far ends of the anchoring needle releasing devices are in preset shapes, the far ends of the conveying pipes can be overall bent or partially bent, the guide rails and the anchoring needles can move towards the far ends of the conveying pipes under the action of external force, and the cardiac valve prosthesis is fixedly arranged between the autologous tissue and the anti-falling ends of the anchoring needles. The novel cardiac valve implantation instrument can achieve accurate locating and firm anchoring, and the treatment success rate is improved.

Medical systems, devices and methods for creation of autologous tissue valves within a mammalian body are disclosed. One example of a device for creating a valve flap from a vessel wall includes an elongate tubular structure having a proximal portion and a distal portion and a longitudinal axis; a first lumen having a first exit port located on the distal portion of the elongate tubular structure; a second lumen having a second exit port located on the distal portion of the elongate tubular structure; a recessed distal surface on the distal portion of the elongate tubular structure, wherein the recessed distal surface is located distally to the first exit port; and an open trough on the recessed distal surface extending longitudinally from the first exit port.

The invention discloses a bioactivity bracket and a preparation method thereof. The bioactivity bracket is obtained through the following steps of firstly, loading bone shape generation proteins on ahollow mesoporous hydroxylapatite microsphere through a grafting manner or an adsorbing manner, so as to obtain a drug bearing microsphere, then mixing the drug bearing microsphere into a bracket matrix solution, then performing printing through a 3D printing technique to prepare a compound bracket, and finally, adsorbing chemotactic growth factors on the surface to obtain the bioactivity bracket;or firstly, blending the drug bearing microsphere and the chemotactic growth factors in the bracket matrix solution, and then performing printing through the 3D printing technique to prepare the bioactivity bracket. Through the adoption of the method disclosed by the invention, the obtained bioactivity bracket can realize time sequence releasing of various growth factors: the chemotactic growth factors are released in an early stage, and bone repair cells are recruited to a damaging region; and bone shape generation proteins are mainly released in the later stage, and bone marrow substrate stem cells which are recruited to the damaging region are induced to be subjected to osteogenic differentiation, so that regeneration and repairing potential of an autologous tissue is sufficiently mobilized, and regeneration and repairing of bone defect are promoted.

Methods and structures are disclosed where carrier fiber is used to enable the assembly of two and three dimensional structures of autologous tissue. Tissue is harvested from the donor, integrated with a carrier fiber, and assembled into complex forms rapidly. The structures can be tailored to the requirements of a specific medical procedure. The tissue is kept live and viable during extracorporeal assembly and the finished structure is emplaced in the donor's body. The use of a carrier fiber leader for pre-threading integration and assembly machines facilitates machine set up, drawing of the tissue into the process, and rapid integration and assembly of the multi-dimensional structures. Assembly can include providing tissue and fiber leaders extending from the structure for attaching the structure in place. The carrier fiber either is bio-absorbed as new tissue forms, or forms a bio-compatible substructure for the patient's native tissue.

Tissue repair in-vivo depends on acute inflammation, but in many clinical situations the other major components of healing such as blood supply, anabolic hormones, growth factors, and stem cells are lacking. This invention includes compositions consisting of an agent which induces an inflammatory healing response combined with an autologous platelet lysate at a specific concentration which may have demonstrated in-vitro abilities to expand autologous tissue repair cells.

The invention relates to a hernia patch, a preparation method and application thereof in herniorrhaphy. The hernia patch is prepared by completely removing all cells possibly causing host immunological rejection from an allogenic skin raw material and integrallty retaining extracellular matrix with the same original tissue structure. The acellular allogenic dermal matrix has tensile strength of 3.0MPa-7.0MPa, stitching strength of 18N-22N, tensile elongation of 10%-30% and bursting strength of 20KPa-30KPa. The hernia patch provided by the invention has the effect of inducing tissue generation,and can be recognized as autologous tissue by human tissue cells after implantation, and soon new blood vessels and fibroblasts can grow into the patch to guide orderly growth of cells along the collagen frame, thus reaching the purpose of supplementation, especially fast repair. The patch is non-degradable within 24 weeks after filling.

The invention includes a composition useful for preparing a tissue sealant for use on a patient, comprising: autologous fibrinogen, an activating agent and at least one supplement. The invention includes a method of treating a disc having at least one defect, comprising: introducing a composition into the disc, wherein the composition comprises autologous fibrinogen and an activating agent, and wherein the composition forms fibrin.

Sterile, virally safe, heterologous, homologous, isologous or autologous tissue, tissue-typed or not tissue-typed, which contains predifferentiated and / or differentiable sessile stem cells and which can be used for wound closure and / or promotion of wound healing.

An implantable prosthesis for percutaneous placement within a vein that forces opposing portions of the vessel wall of a vein together to create a new valve of autologous vein tissue to be operable to alternate between a valve closed configuration and a valve open configuration. When in a preset closed configuration, the implantable prosthesis pushes or pulls portions of the vessel wall of the vein together to substantially close the vein lumen and prevent retrograde blood flow from backflowing through the new valve in the valve closed configuration. The implantable prosthesis has leg portions that may be pushed apart in response to antegrade blood flow through the vein to allow the new valve to achieve the valve open configuration.