741 results about "Extracellular" patented technology

The invention relates to a single cell image segmentation method based on a minimum circumcircle, which solves the technical problem of how to improve the precision, robustness and adaptability of an image-based single cell segmentation method, and comprises the following steps of: firstly, obtaining an original cell image, and secondly, converting the original cell image into a gray level image; preprocessing and binarization processing are carried out on the grayscale image to obtain a binarized image, a cell edge contour image is obtained through processing, a minimum circumcircle is drawn for each contour in the cell edge contour image to obtain a cell circumcircle image, and finally, the center of the minimum circumcircle is used as the center point of a cutting area to obtain a cell edge contour image. And determining the boundary of the cutting area by taking the background including the cells and within a certain range around the cells as a standard, cutting the cell circumcircle image, and finally obtaining a single cell image. The method is suitable for single cell identification and segmentation in a microscope image, and can be widely applied to the fields of cell biology research, medical diagnosis, drug screening and the like.

The invention provides a single cell transcription reaction prediction algorithm based on cross-domain feature cross migration. Drug screening is carried out by using a deep learning technology. The algorithm combines feature decoupling, cross-domain transfer learning and linear modeling methods, and specifically comprises encoder feature decoupling, feature cross transfer, disturbance state modeling and transcriptional spectrum prediction. According to the method, cross-domain transfer learning is applied to the field of drug or gene disturbance-induced cellular response prediction for the first time, and the model is constructed by using gene transcriptional profile data. In the embodiment, the whole genome determination coefficient between the predicted drug perturbation transcriptional profile and the real transcriptional profile reaches 0.81, the differential genome determination coefficient reaches 0.62, efficient prediction of the unicellular external perturbation reaction is successfully achieved, a new thought and method are provided for the field of tumor treatment, and beneficial reference is provided for future drug research and development.

A bioscaffold structure is provided, the bioscaffold comprising a plurality of connected unit cells, each unit cell comprising a plurality of filaments composed of an extracellular material containing Collagen I and Collagen III, wherein each of the plurality of unit cells includes at least one opening connected to an internal volume.

The invention discloses a multi-effect repair type collagen and application thereof, and belongs to the technical field of synthetic biology. Transdermal peptide TD-1, a transmembrane region of human XVII type collagen, an extracellular sixteenth non-collagen region, an extracellular fifteenth collagen region and a coding gene of a His tag are selected to be connected in series, and a gene sequence is optimized through pichia pastoris codon selection preference; and then the T-COL17R3 is constructed and expressed to obtain the T-COL17R3. Efficacy experiments prove that compared with similar products sold in the market, T-COL17R3 not only has excellent transdermal performance, but also has better cell proliferation and migration promoting capability, better capability of resisting early saccharification product ketoamine and later saccharification product dicarbonyl compounds, better capability of scavenging free radicals and resisting elastase, and can be used for preparing the anti-epidermal drug. In addition, synthesis of melanin in B16-F10 cells can be reduced, so that T-COL17R3 has excellent application potential in development of pharmaceutical compositions or skin care products with repairing, anti-wrinkle and firming or whitening and brightening effects.

The invention discloses a light-driven synthetic flora and application thereof. The light-driven synthetic flora comprises phototrophic bacteria and heterotrophic bacteria, the phototrophic bacteria are genetically modified synechococcus slender, and the gene modification comprises construction and expression of heterologous sucrose permease, glyceraldehyde dehydrogenase and sucrose synthase in the synechococcus slender; and the heterotrophic bacteria are the P.adami. According to the light-driven synthetic flora disclosed by the invention, carbon dioxide is converted into cane sugar through photosynthesis of phototrophic bacteria, and the cane sugar is secreted out of cells to provide a carbon source for heterotrophic bacteria; carrying out cell growth and polyhydroxyalkanoate synthesis on the heterotrophic bacteria by using cane sugar; therefore, the light-driven synthetic flora provided by the invention can realize direct conversion of carbon dioxide into polyhydroxyalkanoate without additional carbon sources, has the advantage of environmental friendliness, and provides a new economical and feasible scheme and approach for production of polyhydroxyalkanoate.

The present invention discloses a nano antibody against BCMA and its preparation method and application, and relates to the field of biomedical technology. The present invention uses CRISPR / Cas9 technology to knock out B2M and TRAC genes in T cells to prepare universal CAR-T cells; the nano antibody targeting BCMA and the natural ligand SIRPα extracellular N-terminal V region of CD47, the transmembrane region of CD28, the intracellular signal region of 4-1BB and the intracellular signal region of CD3ζ are connected in series to form a dual-targeted chimeric antigen receptor for the treatment of multiple myeloma, so as to solve the high recurrence problem faced by CAR-T cell therapy in the treatment of multiple myeloma. The universal CAR-T cells prepared by the present invention have strong killing activity against multiple myeloma cells, effectively improve the treatment effect, and provide a key material for the subsequent development of adoptive immunotherapy with high efficiency and lasting anti-tumor activity.

The invention relates to an antibody endocytosis efficiency detection method based on fluorescence resonance energy transfer, and belongs to the technical field of antibody endocytosis detection. The method comprises the following steps: coupling succinimide ester with an amino-containing compound to obtain a quenching agent, incubating an antibody to be detected and a target cell together, adding a fluorescence-labeled secondary antibody to carry out endocytosis reaction, removing an extracellular fluorescence signal by using the quenching agent after the reaction is finished, and detecting an intracellular fluorescence signal to obtain the endocytosis percentage of the antibody to be detected. The detection method provided by the invention is based on a fluorescence resonance energy transfer principle, an intracellular signal direct detection method in the prior art is optimized, and an extracellular fluorescence signal is removed, so that a detection system has higher sensitivity. The quenching agent provided by the invention can also be combined with an acid eluent in the prior art to further reduce an extracellular signal background value and improve the sensitivity of a detection system, can be applied to high-throughput detection of a to-be-detected antibody, is high in sensitivity and low in detection cost, and has a good application prospect.

The invention belongs to the field of cell biology, particularly relates to stem cell exosome repair gel and a preparation method thereof, and more particularly relates to stem cell exosome repair gel containing active biological peptide and a preparation method thereof. The invention provides a repairing composition which comprises an active peptide as shown in SEQ ID NO.1 and a stem cell exosome. The repair gel provided by the invention contains the active peptide and the stem cell exosome loaded with the bioactive substances, and the components have a synergistic effect, so that the expression level of related cell factors can be improved, the repair after skin injury is promoted, and the physiological state of the skin is improved.

The invention relates to the technical field of biomedicine, in particular to stem cell exosome-loaded bi-crosslinked hydrogel as well as preparation and application thereof, and the bi-crosslinked hydrogel comprises the following components in parts by weight: 50-90 parts of a matrix polymer; 0.1 to 5 parts of an active load; 1 to 10 parts of cyclodextrin; 5 to 20 parts of modified chitosan; 0.01 to 1 part of a photoinitiator; and 0.01 to 0.5 part of growth factors. Through double-network design of physical / chemical primary cross-linking and photo-initiation secondary cross-linking, the bottleneck that the mechanical property and the machinability of the traditional single cross-linking hydrogel are difficult to consider at the same time is overcome; meanwhile, the human umbilical vein endothelial cell exosome is introduced, and efficient loading, uniform dispersion and degradation coupling slow release of the exosome are realized by virtue of beta-cyclodextrin inclusion and a double-crosslinking network. Therefore, the problems of mechanics, degradation, cross-linking speed or tissue adhesion of cross-linked hydrogel in the prior art are solved.

Compositions of retroviruses and methods of using the same for gene delivery, wherein the retroviruses comprise a viral envelope protein comprising at least one mutation that diminishes its native function, a non-viral membrane-bound protein comprising a membrane-bound domain and an extracellular targeting domain.

Provided herein are novel stabiligases for use in cell membrane proteome analysis. The subject stabiligases are capable of attaching to glycans found on the surface of cell membranes to form glycan-tethered (GT) stabiligases. Such glycan-tethered stabiligases are capable of robustly and selectively attaching label probes to cell surface proteins of intact cells. The subject novel stabiligases described herein advantageously allow for the identification and profiling of cell membrane proteins that have undergone an extracellular N-terminal proteolytic event.

The invention discloses an MUC1-CAR-T cell for co-expressing a BTLA / IL-18R chimeric receptor as well as a preparation method and application of the MUC1-CAR-T cell, and belongs to the technical field of biological medicines. The MUC1-CAR-T cell comprises a BTLA / IL-18R chimeric receptor and an MUC1-CAR structure, wherein the BTLA / IL-18R chimeric receptor is a BTLA MUC1 is used as a targeting molecule for CAR-T cell therapy, an extracellular domain of BTLA is connected with a transmembrane domain and an intracellular domain of an IL-18 receptor, and the co-expression BTLA / IL-18R chimeric receptor is designed. Experimental verification shows that the BTLA / IL-18R chimeric receptor can significantly improve the tumor killing ability of MUC1-CAR-T cells, can better inhibit the growth of tumors, and provides a novel immunotherapy strategy for the treatment of pancreatic cancer.

In various aspects, the invention provides culture platforms, cell culture media, and methods of differentiating pluripotent cells into hematopoietic cells. In certain aspects, the invention also provides pluripotent stem cell-derived hematopoietic cells generated using the media and methods disclosed herein. The pluripotent stem cell-derived permanent HE cells produced by the methods provided herein are capable of differentiating into hematopoietic lineage cells comprising T cell progenitor cells and T cells in addition to NK cell progenitor cells, NK cells, NKT cells, or B cells.

The present application relates to engineered polypeptides wherein the engineered polypeptides comprise an extracellular targeting domain comprising an epitope (e.g., derived from DLL3, PSMA or SSTR2), an extracellular targeting polypeptide spacer domain, and a transmembrane / membrane affinity domain. Methods of using such engineered polypeptides for detecting, imaging, and treating cancer are also described.

The invention relates to the field of biology, and discloses a cornu cervi pantotrichum stem cell exosome preparation and a preparation method thereof.The preparation method of the cornu cervi pantotrichum stem cell exosome preparation comprises the following steps that after cornu cervi pantotrichum is disinfected, a semitransparent mesenchymal layer at the tip of the cornu cervi pantotrichum is separated out, the cornu cervi pantotrichum is cut into pieces and then flushed with PBS containing double antibodies, the cornu cervi pantotrichum is put into tissue digestion mixed enzyme, and the cornu cervi pantotrichum exosome preparation is obtained centrifuging and cleaning after digestion, and then spreading in a culture bottle for culture to obtain an isolate; when cells of the isolate grow to 70%-90% of a culture bottle, discarding an original culture solution, cleaning with PBS, adding pancreatin for digestion, terminating digestion with a complete medium, and performing cell passage to obtain a passage; and performing gene editing treatment on the cells of the passage. By obtaining the stem cells of the cornu cervi pantotrichum, damage to a donor when the stem cells are obtained by the donor is avoided, and due to the fact that the cornu cervi pantotrichum stem cells have stable biological characteristics, the difference between different sources is reduced, and gene editing is carried out on the subcultured cells.

The invention provides a copper death inducer independent of a copper ion carrier and application of the copper death inducer. According to the novel copper death inducer, copper ions do not need to be additionally added in an extracellular environment. Experimental results show that under the coexistence of the ferroptosis inducer and the ferroptosis inhibitor, the treatment of the glutathione inhibitor can induce the cells to generate obvious death. The cell death can be inhibited by a specific inhibitor TTM of copper death, and has typical forms and biochemical characteristics of copper death, namely oligomerization of DLAT protein. Because copper death and copper death drugs have basic and clinical application prospects, the provided new inducer combination will provide a new small molecule induction strategy and research and application directions for copper death related research.

A method of evaluating disposition and / or effect of a candidate compound in an in vitro culture and / or suspension to predict in vivo disposition and / or effect of the candidate compound, which can include the steps of providing a cell culture and / or suspension; exposing a candidate compound to the culture and / or suspension; exposing the culture and / or suspension to a media providing an in vivo relevant extracellular environment, such as a media comprising a component (such as a protein and / or other component, such as lipoproteins, bile acids, and endogenous compounds such as bilirubin) at a physiologic concentration or a concentration having characteristics, such as binding characteristics, similar to the physiological concentration; wherein any combination of any of the exposing steps can occur in any order or simultaneously; and evaluating in vitro disposition and / or effect of the compound to predict in vivo disposition and / or effect of the candidate compound.

Disclosed in the present disclosure is a stable anti-HER2 bispecific antibody, which comprises a first protein functional region and a second protein functional region used for recognizing extracellular domain II and extracellular domain IV of HER2, respectively. The bispecific antibody remains stable after being stored in PBS at about 37°C for 30 days. Further disclosed in the present disclosure are a pharmaceutical composition containing the bispecific antibody and a PD1 antibody, and the use thereof. The bispecific antibody and the pharmaceutical composition containing same of the present disclosure can be used for treating cancers.

The invention discloses a nano compound based on a CRISPR-Cas9 system and application of the nano compound. The nano compound comprises a histidine oligopeptide-cell penetrating peptide conjugate and an RNP compound composed of sgRNA of a target gene and Cas9 protein, and the histidine oligopeptide-cell penetrating peptide conjugate and the RNP compound are self-assembled under the action of zinc ions to form the nano compound. The nano-composite designed by the invention is simple in structure, and overcomes the defects of high cytotoxicity and large particle size (diameter gt; compared with the prior art, the compound has the advantages that the compound can overcome the defects of complex chemical synthesis, potential immunogenicity and the like, can be used for extracellular-cytoplasm-cell nucleus trinity antivirus, is high in virus removal efficiency and good in antivirus effect, and has high market value and development potential.

Disclosed are systems and methods for detecting extracellular ligands and / or inducing expression of an exogenous or endogenous gene. The disclosed systems and methods typically include and / or utilize (i) first and second exogenous extracellular sensors, and third and fourth exogenous extracellular sensors; and (ii) an expression vector comprising a target gene operably linked to a hybrid promoter sequence. The hybrid promoter sequence of the expression vector includes a minimal promoter for inducing transcription and the hybrid promoter sequence further includes interspaced transcription regulator binding sites upstream of the minimal promoter that bind two or more transcription regulators of the extracellular sensors that are released from the extracellular sensors when the extracellular sensor bind an extracellular ligand.

The invention relates to an IL5RA cell surface marker. A cell tag comprising an extracellular region, a transmembrane region, and optionally an intracellular region is provided. The extracellular region comprises an IL5 receptor alpha (IL5Ra) sequence linked to a transmembrane domain, and the recombinant polypeptide cannot function in signal transduction. The cell tag can be operably linked to a transgene. Expression of the cell tag allows for the identification, detection, selection, and ablation of cells expressing the transgene and the cell tag. In some embodiments, the genetically modified host cell comprises a transgene comprising a polynucleotide encoding a chimeric antigen receptor comprising a ligand binding domain and a polynucleotide encoding a cell tag. Pharmaceutical formulations produced by the methods and methods of using the pharmaceutical formulations are also described.

The invention discloses a composition based on an engineered stem cell exosome and application of the composition in treating male sexual dysfunction. The composition comprises a mesenchymal stem cell exosome modified by genetic engineering, and the surface of the mesenchymal stem cell exosome displays a bifunctional fusion polypeptide targeting TbetaR II and LOXL2. According to the fusion polypeptide, TbetaR-PEP and LOXL2-PEP are integrated through a flexible linker, and an HIV-TAT penetrating peptide and a self-assembly motif are introduced, so that nanoparticles are formed, and the half-life period is prolonged. In-vitro experiments show that the inhibition efficiency of the fusion polypeptide on TbetaRII and LOXL2 is obviously superior to that of a single peptide, and the fusion polypeptide can synergistically reverse fibrosis and promote angiogenesis. Animal experiments prove that the exosome loaded with the fusion polypeptide can significantly improve the erectile function of diabetic ED rats. According to the invention, a radical treatment scheme is provided for male sexual dysfunction through double-target cooperation, long-acting delivery and a multi-effect repair mechanism.

Provided herein is a sterile decellularized placental derived extracellular connective tissue matrix (SDPECTM) having applications in wound management and aesthetics.

The invention belongs to the technical field of cell culture, and particularly relates to a culture method for in-vitro amplification of NK cells activated by NK cell exosomes, which comprises the following steps of: after promoting the NK cells to generate a large amount of exosomes by using an electrical stimulation method, filtering, performing ultrafiltration, extracting and purifying the NK cell exosomes by using specific immunomagnetic beads, thereby obtaining the NK cell exosomes activated by the NK cell exosomes. A set of strict detection and quantification standards is established for the exosome by utilizing Western Blot, a BCA method, DLS and NTA technical means, a primary PBMC is obtained by adopting a density gradient centrifugation method, and the high-activity and high-purity NK cell exosome is dynamically added into a cell culture medium in the activation and amplification stages of the PBMC, so that self-activation of the NK cells is realized, the proliferation efficiency of the NK cells is remarkably improved, and the NK cell exosome has a good application prospect. Meanwhile, the safety risk of a culture system is reduced.

A mediated extracellular electron transfer as a rapid and direct method to classify antibiotic-susceptible and -resistant bacteria is disclosed herein. Particularly, devices and methodologies to interpret antibiotic susceptibility index of a known bacteria strain or uncharacterized bacteria by treating it with antibiotics of different mechanisms or different concentrations of an antibiotic is provided. In an example methodology, the process includes a number of current control signal responses and a number of inoculated current response signals and thereafter analyzing the inoculated current response signals over a range of concentrations of up to about 24 times a breakpoint of the antibiotic with respect to current control signal responses to provide a susceptibility index assessment indicative of the susceptibility or resistance of the pathogen to the antibiotic.

The invention discloses a nano antibody and a preparation method and application thereof, the nano antibody comprises a heavy chain variable region, the heavy chain variable region comprises CDR1, CDR2 and CDR3, and the amino acid sequence of the heavy chain variable region of the nano antibody comprises SEQ ID NO: 3. The nano antibody provided by the invention is determined as a competitive OTR inhibitor with extracellular treatment potential; in-vitro and in-vivo experiments prove that the nano antibody has a curative effect equivalent to that of atosiban which is widely used clinically, and a new direction is provided for developing effective anti-premature delivery medicines.

The invention provides a grifola frondosa liquid culture medium as well as a culture method and application thereof, and relates to the technical field of fermentation. The invention provides a grifola frondosa liquid culture medium, which contains glucose, peptone, KH2P2O4, MgSO4, vitamin B1, gibberellin and tyrosol, can significantly improve the growth of grifola frondosa thalli and the secretion of exopolysaccharides, and can be used for the preparation of food, drugs and health care products.

The invention belongs to the field of nucleic acid editing, and particularly relates to the technical field of regularly clustered interval short palindromic repeat (CRISPR). Specifically, the invention provides a novel Cas enzyme, the homology of the Cas enzyme and the reported Cas enzyme is low, the activity of nuclease can be shown in and out of cells, and the Cas enzyme has a wide application prospect.

The invention relates to the field of microorganisms, in particular to anti-radiation acinetobacter ZXP-05, a heavy metal adsorbent and application of the anti-radiation acinetobacter ZXP-05 and the heavy metal adsorbent. The invention provides a strain of acinetobacter radioresistenes ZXP-05, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.32490. The invention also provides a method for preparing the acinetobacter radioresistenes ZXP-05. The anti-radiation acinetobacter ZXP-05 provided by the invention can participate in heavy metal adsorption through intracellular and extracellular combined action, and extracellular adsorption is superior to intracellular adsorption. In the specific embodiment of the invention, a plurality of studies show that the anti-radiation acinetobacter ZXP-05 has good resistance and adsorption capacity to Cd < 2 + > and Ni < 2 + >; in the heavy metal adsorption process, amino groups, hydroxyl groups, carboxyl groups, phosphate groups and the like on cells of the strain play an important role; the cells are severely recessed along with the increase of Cd < 2 + > concentration, and the adhesion is enhanced along with the increase of Ni < 2 + > concentration.

The present disclosure provides an attention-based MIL model for use in extracting and characterising cell features, at both the cell level and the population level. To characterise cells, cells in a well are initially fed into the model. The cells in a well include a points cloud of cells and a point cloud of corresponding cell nuclei. The cells in the well are passed through a pretrained DFN encoder which extrapolates the cell and nuclei features from the cells in the well. These extracted features are then passed through a transformer based encoder to produced transformed versions of the extracted cell and nuclei features. These transformed features are then fed into at least two classifiers. One of the classifiers is a cell-level MLP classifier which is used to compare the cell features with known drug response to attempt to match a phenotype signature with a known drug response. One other classifier is the bag classifier which is used to compare the cell population features with that of known drug responses, again to attempt to match to a phenotype signature. The outputs of the cell classification and the bag classification are used to label the cells in the well with a drug label or a potential drug label / use case.