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Reparative cell delivery via hyaluronic acid vehicles

a technology of hyaluronic acid and reparative cells, applied in the direction of skeletal/connective tissue cells, prosthesis, drug compositions, etc., can solve the problems of the inability to apply the above mentioned advances in the field of reparative and regenerative cell populations, and the inability to achieve the functional restoration of compromised tissues and organs

Active Publication Date: 2011-03-24
BOARD OF RGT THE UNIV OF TEXAS SYST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

"The present invention provides methods for treating various tissue defects by using a stabilized autologous cell graft made by mixing autologous cells with hyaluronic acid or a hyaluronic acid derivative. The graft can be generated at a point-of-care and implanted into a patient without culturing the cells. The graft can also be incubated with inductive media before, during, or after mixing with hyaluronic acid or a hyaluronic acid derivative to generate different types of cells such as adipocytes, chondrocytes, endothelial cells, hepatocytes, myoblasts, neurons, and osteoblasts. The technical effects of this invention include improved regenerative cell therapy for tissue defects and a simplified and efficient method for generating a stable cell graft for treatment purposes."

Problems solved by technology

Despite the availability of biocompatible grafts, functional restoration of compromised tissues and organs presents a ongoing challenge in medicine.
However, practical application of the above mentioned advances has heretofore been unavailable for various reasons to be explained in more detail in the context of the solutions provided herein.

Method used

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  • Reparative cell delivery via hyaluronic acid vehicles
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  • Reparative cell delivery via hyaluronic acid vehicles

Examples

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example 1

[0041]To explore the potential for a role for SVF cell in soft-tissue repair, the present inventors investigated the ability of the ADSC subset of SVF cells to proliferate and differentiate when exposed to hyaluronic acid compared with growth of ADSC under standard culture conditions. It was also investigated whether the mixture had a collagen-inducing effect in vitro.

[0042]As disclosed herein, the present inventors have demonstrated that a commonly used injectable soft-tissue NASHA filler is compatible with tissue-resident MSCs derived from adipose tissue. The ADSCs were shown to proliferate equally when grown on HA or NASHA substrates compared to standard tissue culture-conditions on a plastic substrate. Real-time PCR showed that procollagen gene expression is increased in ADSC cultures in conjunction with HA. Importantly, in vivo studies showed a significant increase in vascular density at the site of ASC-NASHA grafts was shown as compared with NASHA alone. A robust vascular supp...

example 2

[0065]It has been shown that the phenotype of plastic adherent adipose derived cells changes with cell culture and is influenced by culture conditions. (Gimble J and Guilak F. “Adipose-derived adult stem cells: isolation, characterization, and differentiation potential”Cytotherapy 5(5) (2003) 362-369; Boquest A C, et al “Isolation and transcription profiling of purified uncultured human stromal stem cells: Alteration of gene expression after in vitro cell culture”Mol. Biol. Cell 16(3) (2005) 1131-1141). Use of reparative cell preparations such as freshly derived SVF populations for incorporation into HA fillers would avoid the need for culture with its attendant disadvantages and would permit utilization of larger cell numbers including cells that would ultimately exhibit the properties of plastic adherent stem cells if given the chance but are discarded using standard stem cell isolation procedures that call for the discard of all cells that are not adherent at 24 hours of culture....

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Abstract

Methods are described for generating autologous tissue grafts, including generating grafts at the point of case, which include isolated cell populations that are enriched with stem cells and are mixed with hyaluronic acid and derivatives thereof. The hyaluronic acid localizes the cells to a desired injection site and stimulates collagen production thus enhancing the viability and the longevity of the graft.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application claims priority to U.S. Application Ser. No. 61 / 172,488, filed on Apr. 24, 2009. The disclosure of the prior application is considered part of (and is incorporated by reference in) the disclosure of this application.FIELD OF THE INVENTION[0002]This invention relates to compositions and methods for delivery of reparative and regenerative cell populations in the treatment of tissue injuries and defects.BACKGROUND OF THE INVENTION[0003]Without limiting the scope of the invention, its background is described in connection with novel compositions and methods for therapeutic delivery of reparative cell populations in the treatment of soft tissue loss and disfigurement due to pathologic, traumatic, and aging-related processes. Scaffold-based biocompatible prosthetic grafts have been used with some success for restoring soft tissue loss. See e.g. Butler, C. E. The role of bioprosthetics in abdominal wall reconstruction. Clin. Pla...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K35/12A61F2/00A61P17/02A61P19/08A61P19/04A61P27/02A61P9/10A61P25/00A61P1/00A61P19/02
CPCA61K35/12A61L27/18A61L27/38A61L2400/06C12N2533/80C12N5/0667C08L67/04A61P1/00A61P17/02A61P19/02A61P19/04A61P19/08A61P25/00A61P27/02A61P9/10A61L27/20A61L27/24A61L27/3604A61L27/3662A61L27/3679A61L2300/64A61L2430/10A61L2430/22
Inventor ALTMAN, ANDREW M.ALT, ECKHARD U.COLEMAN, MICHAEL E.
Owner BOARD OF RGT THE UNIV OF TEXAS SYST
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