100 results about "Cell delivery" patented technology

The invention relates to the field of hydrogel preparation, and discloses anti-scale zwitter-ion hydrogel as well as a preparation method and application thereof. The method comprises the following steps: firstly, initiating an oxazine derivative monomer to polymerize through a double-end initiator to generate dihydroxyl polyoxazine; and then, removing a side chain of the polymer to obtain the dihydroxy polypropylene imine. Then, dihydroxy polypropylene imine is selected for further reaction to generate a zwitter-ion precursor polymer, and the zwitter-ion precursor polymer is hydrolyzed to obtain the polypropylene imine zwitter-ion derivative. And finally, converting hydroxyl groups at two ends, synthesizing a zwitterionic macromolecular cross-linking agent, and carrying out polymerization cross-linking in an aqueous solution to prepare the anti-scale zwitterionic hydrogel. The hydrogel is simple in formula, is completely composed of the zwitterionic cross-linking agent, has excellent anti-scaling performance such as protein adsorption resistance, bacterial adsorption resistance and cell adsorption resistance, and is suitable for biological materials, medical devices, implant materials and cell delivery materials.

The invention relates to a preparation method of core-shell nanoparticles with photo-thermal-phase change dual functions and intracellular delivery application of the core-shell nanoparticles, and belongs to the field of nano materials and cell delivery. The invention provides a photothermal-phase change bifunctional core-shell nanoparticle applied to intracellular delivery of biomacromolecules, the nanoparticle is of a core-shell structure, a high-molecular polymer is taken as a shell, liquid fluorocarbon and a photothermal material are taken as a core, the nanoparticle is prepared by an ultrasonic emulsification method, and the preparation method is simple. The boiling point of the liquid fluorocarbon is low, and the photo-thermal material has good photo-thermal conversion performance, so that the core-shell nanoparticles can quickly generate photo-thermal steam microbubbles under laser induction. Under the synergistic effect of laser and core-shell nanoparticles, generated steam microbubbles are induced to generate micropores in plant cell walls and cell membranes or animal cell membranes, so that the physical barrier of the cell walls and the cell membranes is overcome, and the efficiency of delivering exogenous functional biomacromolecules into cells is remarkably improved.

The present invention relates to a stretchable self-healing hydrogel. The hydrogel has excellent mechanical properties and stability, and stretchable and self-healing properties, and can be useful as a hydrogel for delivery of a drug or a cell, and a composition for 3D bioprinters.

Described herein are compositions comprising at least two diblock copolypeptides, wherein each copolypeptide has an ionic segment with the opposite charge from the other. The copolypeptides form β-sheet structured hydrogel assemblies via polyion complexation when mixed in aqueous media. As a result of their unique physical properties, the hydrogels are useful as cell suspension or cell culture media, in drug or cell delivery systems, in scaffolds for tissue repair, or as 3d-printable media.

Provided herein are methods of preparing an engineered heart valve for implantation into a subject in need (e.g., a pediatric patient). For example, a porcine pulmonary valve can be decellularized and then recellularized with cells from the subject. Hydrogels can be used to facilitate cell delivery and growth within the sub-endothelial layer (e.g., spongiosa) of a heart valve, which is essential for long-term success of the implantation.

The present invention relates to a novel ionizable lipid compound represented by formula (I), or a salt thereof, and lipid nanoparticles comprising same. The lipid nanoparticles comprising the novel ionizable lipid compound, according to the present invention, have excellent nucleic acid encapsulation efficiency and high nucleic acid cell delivery efficiency.

The present disclosure is directed to various compositions that include one or more energy source components; one or more pH buffers; one or more salts; and one or more stabilizing agents and one or more cell types.

[Problem to be Solved]Provided is a delivery carrier into the cell having high antioxidant activity, intracellular absorbability, intracellular disintegration property, stability and safety, which have high delivery property of the active ingredient to cell and living tissues.[Solution]The delivery carrier into cells includes a vitamin derivative with co-activation of both autophagy-related genes and protease synthesis genes, a polymer molecule containing stimulatory reactivity, an emulsion stabilizer, an active ingredient, and a lipid, thereby providing a delivery carrier into cells with high delivery properties of the active ingredient to cells and living tissues.

The present invention provides a method and a corresponding device for controlling a micro-nanorobot cluster, which adopts a driving method based on a rotating gradient magnetic field to drive a micro-nanorobot cluster with magnetic components as a carrier for precise drug or cell delivery. The present invention uses a plurality of electromagnetic coils driven by a programmable timing electrical signal, which can generate an equivalent centripetal force pointing to the target position in the working space area, thereby attracting the micro-cluster to converge from different directions to the target position including the diseased tissue. By changing the current input of the electromagnetic coil, the coordinates of the target position can be adjusted.

Described herein are compositions, kits, and methods for potent delivery to a cell of a subject. The cell can be of a particular cell type, such as a basal cell. In some cases, the cell can be a lung cell of a particular cell type. Also described herein are pharmaceutical compositions comprising a therapeutic or prophylactic agent assembled to a lipid composition. The lipid composition can comprise an ionizable cationic lipid, and a selective organ targeting lipid. The lipid composition can further comprise a phospholipid. Further described herein are high-potency intravenous dosage forms of a therapeutic or prophylactic agent formulated with a lipid composition.

A method of preparing a syringe in connection with a therapeutic treatment is disclosed. The method can include removing a plunger of the syringe from a barrel of the syringe, aligning the barrel in a horizontal orientation, filling a lumen of the barrel with a viscous material through an opening at a proximal end of the barrel, and inserting a plunger tip into the lumen to seal the lumen. The method can also include attaching an implantation device to a hub coupled to the barrel at a distal end of the barrel. The method can also include depressing the plunger until the cell suspension fills the implantation device and a droplet of the cell suspension is expelled from a distal tip of the implantation device.

Provided herein are intravascular retrievable cell delivery systems and methods of use thereof for cell transplantation.

The present invention relates to the field of the synthesis of polymers. Disclosed are a polypropyleneimine derivative, and a preparation method therefor and a use thereof. The method comprises: firstly, initiating polymerization of an oxazine derivative monomer by means of an initiator, then removing a side chain from a polymer to obtain polypropyleneimine, selecting the polypropyleneimine for a further reaction to obtain a zwitterionic precursor polymer, and finally, hydrolyzing the precursor polymer to obtain a polypropyleneimine zwitterionic derivative. The polypropyleneimine zwitterionic derivative prepared in the present application contains a zwitterionic group in each or some of repeating units. The zwitterionic group endows the polymer with good hydrophilicity and anti-protein non-specific adsorption properties. The polymer has no immunogenicity, will not be recognized by the immune system and captured by immune cells, can be used as a drug carrier and a gene vehicle, and has good blood circulation stability. Alternatively, the polymer is used as a biological material, a medical device, an implantable material, or a cell delivery material and has good anticoagulation, anti-rejection, and biofilm properties.

The invention relates to the technical field of medical engineering, in particular to an NK cell delivery data processing method and system based on magnetic targeting. The method comprises the following steps: inquiring an immune receptor protein structure of natural killer cells; injecting a sodium chloride solution, adding an immune receptor protein structure, and reacting to generate an aptamer structure; the method comprises the following steps: combining streptavidin in a pre-prepared glycosyl magnetic microsphere to obtain a high-affinity magnetic bead; carrying out molecular docking on the high-affinity magnetic beads and the aptamer structure, recording a docking score, and generating an optimized aptamer; recording the magnetic bead mass ratio of the optimized aptamer; capturing the natural killer cells by adopting the optimized aptamer, and shooting to obtain a fluorescent microscopic image; and recording the dyeing condition of the fluorescent microscopic image, determining the survival state of the NK cells, marking the position information of the living cells, and generating an NK cell delivery efficiency table by using the mass ratio of the magnetic beads. Based on a medical engineering technology, the homing rate and the delivery efficiency of the NK cells at the liver injury part are improved.

The present disclosure relates to methods and compositions comprising a universal cell delivery ligand configured to enhance the intratumoral delivery and distribution of therapeutic immune cells by facilitating their penetration through dense stroma surrounding tumor cells.

This invention belongs to the field of biomedical technology, specifically relating to lipid nanoparticles, their preparation method, and their application in immune cell delivery. This invention introduces amino acid-derived lipids as a fifth component into a preferred four-component formulation. Furthermore, through screening of the nitrogen-to-phosphorus ratio, the proportion of amino acid-derived lipids introduced, and the type of amino acid-derived lipids, novel lipid nanoparticles capable of efficiently delivering primary immune cells are selected. The delivery carrier described in this invention can significantly improve the delivery efficiency to primary immune cells, especially NK cells, providing a safe and efficient tool for the engineering of immune cells and their clinical application in immune cell therapy.

The invention provides a Motixazotide-nitrogen mustard conjugate and a preparation method and application of a fluorescent probe of the Motixazotide-nitrogen mustard conjugate, and belongs to the field of polypeptide preparation and biological medicine. According to the present invention, a solid phase polypeptide synthesis method is adopted to covalently link a DNA alkylation reagent nitrogen mustard and a CXCR4 targeting peptide Motixazotide, and further couple with a fluorescent dye so as to successfully prepare a series of novel conjugates and fluorescent probes thereof; experiments prove that the Motixazotide-nitrogen mustard conjugate and the fluorescent probe thereof prepared by the invention can be used for remarkably improving the anti-tumor activity of nitrogen mustard and the targeting property of the nitrogen mustard to tumor cells. Meanwhile, the rhodamine B labeled dinitrogen mustard conjugate BCCR has a specific targeting effect on a CXCR4 receptor and a dual targeting effect on a tumor cell nucleus. The conjugate realizes real-time tracing of the whole process of tumor targeting, cell delivery and nuclear localization, and provides a powerful tool for curative effect evaluation and mechanism research, so that the conjugate has good clinical transformation prospect and application value.

The invention relates to the field of gene therapy. In addition the invention relates to the field of interfering RNA and / or microRNA (miRNA). In particular the invention relates to gene therapy involving such miRNA's and more in particular to methods and means to improve delivery of said miRNAs to target cells of a patient. The invention provides for a gene delivery vehicle for use in delivery of a miRNA to a cell resulting in silencing of a desired gene and whereby spread of said miRNA to other non-transduced cells results in silencing of said desired gene in said non-transduced cells.

The invention belongs to the technical field of biological medicine, and particularly relates to lipid nanoparticles, a preparation method thereof and application of the lipid nanoparticles in immune cell delivery. According to the invention, amino acid derived lipid is introduced as a fifth component on the basis of a preferable four-component formula. Through screening of the nitrogen-phosphorus ratio, the introduction proportion of the amino acid-derived lipid and the type of the amino acid-derived lipid, the novel lipid nanoparticles capable of efficiently delivering the primary immune cells are preferably selected. According to the delivery carrier, the delivery efficiency of primary immune cells, especially NK cells, can be remarkably improved, and a safe and efficient tool is provided for clinical application of immune cell engineering and immune cell treatment.

The present invention provides recombinant TIM-4 fusion proteins comprising a domain of TIM-4 and a scFv specific for CD3 and methods of making and using the same. The fusion proteins provided herein may be administered in combination with CAR T cells. In addition CAR T cells engineered to express the TIM-4 fusion proteins described herein are also provided and may be used in the methods described herein.

The invention provides an enzyme-residue-free extracellular matrix microsphere as well as a preparation method and application thereof. An elutable sacrificial phase material capable of being subjected to liquid-liquid phase separation with an extracellular matrix is introduced in an extracellular matrix pelletizing process, and transglutaminase is confined in a sacrificial phase, so that the extracellular matrix microsphere is prepared. And the transglutaminase and the extracellular matrix form a spatially separated structure in the balling process. In the gel forming stage, transglutaminase catalyzes protein in an extracellular matrix on a two-phase interface to generate a cross-linking reaction, and microspheres with a three-dimensional network gel structure are formed; after cross-linking is completed, transglutaminase is removed by eluting the sacrificial phase material, and meanwhile, a porous network with a communicated structure is formed in situ in the microsphere, so that the obtained extracellular matrix microsphere still keeps good structural integrity and mechanical property while realizing no residue of enzyme with catalytic activity; the scaffold can provide stable support for cell adhesion, migration and three-dimensional culture, and is applicable to cell delivery, organoid construction, tissue engineering scaffolds and the like.

Provided herein are, inter alia, extracellular products (e.g., vesicles such as microvesicles, e.g., exosomes) produced by renal cells (such as bioactive renal cells, e.g., selected renal cells). Methods of altering components (such as miRNAs or proteins) of vesicles produced by cells, as well as methods of producing vesicles comprising various compounds are also included. Also provided are diagnostic and treatment methods.

Engineered cells that serve as vehicles to deliver genetically-encoded cargo molecules, such as therapeutic molecules, to other cells in vivo are provided. In particular, fusogenic biovesicles with reduced immunogenicity potential, expressing poorly immunogenic (fully human or humanised or deimmunised) fusogens enclosing the cargo molecules are produced by the engineered cells. The engineered cells can be used in methods of treating diseases and conditions such as amyotrophic lateral sclerosis, Alzheimer's disease and cancer.

Disclosed are cell encapsulation devices and methods for transplanting cells, such as pancreatic endoderm cells, into a host. In some examples, a cell encapsulation can comprise a lumen configured to receive cells therein, a cell-excluding membrane, where the lumen is internal to the cell-excluding membrane, and a non-woven fabric layer external to the cell-excluding membrane, where the non-woven fabric layer and the cell-excluding membrane comprise perforations. The device can further comprise a woven mesh external to the non-woven fabric layer, where the non-woven fabric layer provides protection to the cell-excluding membrane from direct contact with the woven mesh.

The present invention relates to an immunoprotective type implantable cell delivery device for implanting cells in a subject comprising an enclosed space between a top and bottom porous membrane and a mesh. The mesh is used to prevent clustering of the cells. The invention further relates to a method of constructing the closed type implantable cell delivery device. The invention also relates to the device for use in a method of treatment.

The present disclosure provides a cargo delivery fusion polypeptide comprising an endosomolytic polypeptide and a cell penetrating polypeptide; and compositions comprising the cargo delivery fusion polypeptide. The present disclosure also provides methods of delivering a cargo into a target eukaryotic cell, using a composition comprising an amphiphilic polypeptide.

An isolated, non-naturally occurring cell penetrating peptide (CPP) comprising the amino acid sequence: RRSRTARAGRPGRNSSRPSAPR [SEQ ID NO: 1] and sequences having at least 60% similarity to SEQ ID NO: 1.

The present disclosure presents hydrogel compositions for use in the treatment of an ocular disorder (e.g., a retinal disease) and methods of treating an ocular disorder in a subject in need thereof with the hydrogel compositions. The hydrogel compositions can include hydroxyphenylpropionic acid (gelatin-HPA), hyaluronic acid-tyramine (HA-Tyr), a catalyzer (e.g., horseradish peroxidase (HRP)), a cell, a crosslinker (e.g., hydrogen peroxide), or any combination thereof. The methods include administering a therapeutically effective amount of a composition into an eye of the subject, wherein the composition includes any of the hydrogel compositions of the disclosure.

The present disclosure presents hydrogel compositions for use in the treatment of an ocular disorder (e.g., a retinal disease) and methods of treating an ocular disorder in a subject in need thereof with the hydrogel compositions. The hydrogel compositions can include hydroxyphenylpropionic acid (gelatin-HPA), hyaluronic acid-tyramine (HA-Tyr), a catalyzer (e.g., horseradish peroxidase (HRP)), a cell, a crosslinker (e.g., hydrogen peroxide), or any combination thereof. The methods include administering a therapeutically effective amount of a composition into an eye of the subject, wherein the composition includes any of the hydrogel compositions of the disclosure.

The present invention includes compositions and methods for modifying a T cell with a nucleic acid encoding a switch molecule comprising an extracellular domain comprising a membrane receptor or fragment thereof and an intracellular domain comprising a signaling receptor or fragment thereof. In one aspect, a method comprises introducing a nucleic acid encoding a switch molecule and a nucleic acid encoding a soluble fusion protein and / or a nucleic acid encoding a bispecific antibody into a population of cells comprising T cells, wherein the T cells transiently expresses the switch molecule and soluble fusion protein or bispecific antibody. In other aspect, compositions of T cells and methods of treating a disease or condition, such as cancer or an autoimmune disease, are also included.