28 results about "Barrier integrity" patented technology

The invention discloses a preparation method of a cochlear organ chip integrated with a blood labyrinth barrier, and belongs to the field of biomedical engineering. The preparation method of the simulated blood labyrinth barrier comprises the following steps: constructing an endothelial cell culture cavity and a pericyte culture cavity, and separating the endothelial cell culture cavity and the pericyte culture cavity through a simulated basement membrane; and respectively inoculating percutaneous cells and endothelial cells into a percutaneous cell culture cavity and an endothelial cell culture cavity, and culturing to obtain the simulated blood lost barrier. The chip can effectively simulate the structure and the function of the BLB, and the barrier integrity of the BLB is evaluated through TEER (trans-epithelial electrical resistance) measurement and an apparent permeability coefficient. The invention also relates to application of the chip in NIHL drug screening, in particular to drug evaluation for oxidative stress induced inner ear organ injury. Through the platform, the protection effect of the candidate drugs on the TBHP-induced oxidative stress injury of the inner ear organs can be evaluated, and a new tool and thought are provided for developing novel NIHL treatment drugs.

The invention discloses an application of a compound in preparation of a medicine for protecting pulmonary vascular endothelial cells, and relates to the technical field of medicines, the application of the compound in preparation of the medicine for protecting the pulmonary vascular endothelial cells comprises the compound, and the compound is used as an active component of the medicine. The compounds are useful as inhibitors of OSM causing barrier damage to endothelial cells; the compound is used for inhibiting OSM-induced vascular endothelial cell permeability increase in drugs. The compound is used for improving the reduction of vascular epithelial cell membrane electrical impedance caused by OSM in medicines, so that the integrity of a cell barrier is protected; the compound is used for up-regulating vascular endothelial cadherin expression and reducing vascular endothelial permeability in medicines, and repairing vascular endothelial barrier damage caused by OSM. The compound can reverse the reduction of OSM-induced endothelial adhesion connexin and protect the damage of an endothelial barrier, and has a remarkable application prospect in medicines for protecting pulmonary vascular endothelial cells.

The application discloses a plant lactobacillus strain and application thereof, and belongs to the technical field of microorganisms. The preservation number of the strain is CGMCC No. 33864. The strain has good tolerance to gastrointestinal juice and bile salts and has no hemolytic activity. Concentrated cell-free supernatant prepared from the strain can inhibit salmonella, including inhibiting the growth of salmonella, killing bacteria, inhibiting or removing biofilm. Animal experiments show that the strain and the concentrated cell-free supernatant thereof can prevent salmonella infection, alleviate weight loss caused by infection, maintain intestinal barrier integrity, reduce the level of pro-inflammatory factors, alleviate tissue damage, improve the expression of colon tight junction proteins and mucin, promote the generation of short-chain fatty acids, and restore the intestinal flora structure. The application further provides a microbial preparation containing the strain and / or the concentrated cell-free supernatant thereof. The strain and the microbial preparation can be used for preparing products for resisting salmonella, preventing salmonella infection or improving intestinal health.

The invention relates to the technical field of biomedicine, and particularly provides a mouse model capable of being inductively combined with epithelial cell specific ablation and application of the mouse model. The method comprises the following steps: inserting a '3xFLAG-P2A-CreERT2 'functional frame at a fixed point at a termination codon of a mouse Odam gene, so as to obtain an OdamCreERT2 mouse. The expression specificity of the Odam gene in binding epithelium is verified by a mouse obtained by copulating with a ROSA26mT / mG reporter gene mouse; mice obtained by mating with ROSA26DTA cell ablation mice reveal the indispensable function of binding epithelium in maintaining periodontal barrier and preventing bone resorption. The model retains endogenous Odam expression and functions, supports protein dynamic tracing and space-time specific gene manipulation, and is suitable for researching the effect of binding epithelium in periodontal barrier integrity and alveolar bone resorption prevention.

The invention discloses a probiotic composition, fermented yoghurt and application of the probiotic composition and the fermented yoghurt in improving related indexes of athletes. The probiotic composition comprises bacillus subtilis SP-8-5, lactobacillus plantarum YM-4-3, lactobacillus plantarum AY01 and pediococcus acidilactici LH01. The composition of intestinal microbial populations can be adjusted through a specific compounding method, the abundance of harmful bacteria is reduced, the abundance of beneficial bacteria is improved, the immunity of intestinal tracts is enhanced, and the integrity of intestinal barriers of athletes is maintained. Meanwhile, the composition has a positive improvement effect on related exercise indexes such as plasma amino acid, excrement metabolites and exercise performance, and can be used as an effective exercise supplement to be applied to athletes.

A system and method for measuring, forecasting, and modulating aqueous outflow is described here. The system and method operate on an ocular microphysiological system that reproduces the trabecular-meshwork-membrane-Schlemm's canal interface under a defined hydrodynamic program. The signals may include TEER resistance, pressure-flow measurements, OCT / OCTA images, and Raman spectra. The Ocular MPS system may include encoding the multimodal signals into a device-agnostic feature representation that includes descriptors of junction continuity, belt thickness, tortuosity, pathway activity, and outflow-resistance proxies. The Ocular MPS system may execute a physics-informed graph state-space model that fuses the device-agnostic feature representation into inferred parameters. The inferred parameters may be inferred from barrier integrity, permeability, and outflow facility, subject to monotonic constraints between structural and hydraulic variables. The Ocular MPS system may quantify uncertainty of inferred parameters, generating calibrated forecasts of aqueous outflow performance. The Ocular MPS system may control a drive actuator according to the calibrated forecasts.

The present invention relates to probiotic compositions comprising at least one Lactobacillus helveticus, at least one Bifidobacterium longum, and at least one Bifidobacterium animalis. The present invention further relates to methods of using said compositions for preventing muscle loss, improving or maintaining muscle strength, and maintaining intestinal barrier integrity.

The application belongs to the technical field of biological medicine, and particularly relates to umbilical cord mesenchymal stem cells and a preparation method and application thereof. The preparation method takes umbilical cord Wharton's jelly as raw material, and obtains high-purity umbilical cord mesenchymal stem cells through cleaning treatment, tissue block preparation, serum-free culture and liquid change subculture. The culture conditions and operation parameters are strictly controlled in the preparation process, the MSC serum-free culture medium and trypsin substitutes are used, so that the cell activity and purity can be guaranteed. The umbilical cord mesenchymal stem cells have no obvious toxicity and tumorigenic risk under a safe dose, and have no damage to organs in long-term use. The umbilical cord mesenchymal stem cells can significantly improve the motor dysfunction after cerebral ischemia, reduce the volume of cerebral ischemic injury, protect the integrity of the blood-brain barrier, reduce the neuroinflammatory response, and play a multi-dimensional role in brain tissue protection and repair, thereby providing a new effective means for the treatment of ischemic stroke, and having important clinical transformation value and application prospect.

The invention belongs to the technical field of biological agents, and particularly relates to application of Roseburia inulinivorans in preparation of a medicine for treating and / or preventing enteritis. The invention finds that the intestinal bacteria Roseburia inulinivorans can improve ulcerative colitis by adjusting the steady state of microorganisms, protecting the integrity of intestinal barriers and maintaining the steady state of immunity. According to the application, the curative effect of Roseburia inulinivorans on colitis is comprehensively evaluated, and the Roseburia inulinivorans has the functions of resisting inflammation, recovering immune balance, resisting oxidative stress, resisting pyroptosis, regulating bile acid metabolism, relieving endothelial dysfunction and intestinal ecological dysfunction and the like, and particularly, the Roseburia inulinivorans can achieve the treatment effect the same as that of a clinical conventional medicine mesalazine. And meanwhile, the Roseburia inulinivorans has no side effects, so that the Roseburia inulinivorans have no side effects. Therefore, the Roseburia inulinivorans is very likely to become a good candidate drug for improving the ulcerative colitis.

The invention relates to the technical field of biological medicines, and particularly discloses a method and a composition for promoting intestinal absorption of dihydroquercetin by using quercetin and application of the method and the composition, and the method comprises the following steps: jointly applying an effective amount of dihydroquercetin and an effective amount of quercetin serving as a synergist to an intestinal environment; wherein the quercetin plays a role through at least one of the following mechanisms: inhibiting the function or expression of P-glycoprotein in intestinal epithelial cells; the tight connection of intestinal epithelial cells is regulated. It is found that quercetin not only can effectively inhibit the excretion function of P-glycoprotein in intestinal epithelial cells, but also can enhance the integrity of intestinal barriers by down-regulating the expression level of the protein and up-regulating the expression of tight junction protein, so that intestinal absorption of dihydroquercetin is synergistically promoted from the multi-mechanism level; in addition, the composition can still maintain a good absorption promoting effect in an inflammatory environment, and shows a synergistic anti-inflammatory effect.

The invention belongs to the field of medicine and health, and particularly relates to application of fresh traditional Chinese medicine radix puerariae exosome-shaped vesicles P-ELNs in medicine or health food for preventing and treating irritable bowel syndrome, the P-ELNs serving as an active ingredient can improve expression of colonic intestinal mucus protein MUC2 and tight junction protein ZO-1 and improve mucous membrane barrier integrity; the inhibition of the expression level of proinflammatory factors (such as TNF-alpha, IL-6, IL-1beta and NF-kappa B) indicates that the action mechanism may involve the synergistic effect of inflammation inhibition and barrier repair. Toxicological experiments show that the P-ELNs have no toxicity after long-term intragastric administration, have no adverse effects on heart, liver, spleen, lung and kidney functions, and have good oral safety and tissue tolerance. The indications comprise anti-inflammatory treatment of active-stage UC patients; maintenance and barrier repair of patients in the UC remission period; the UC disease course is more than or equal to 8 years, and a preventive medicine is applied to high-risk people with cancerization family history and the like; the invention relates to auxiliary treatment of UC-CAC early cancerization patients.

The application belongs to the technical field of biological medicine, and particularly relates to application of phosphatidylcholine in preparation of a medicine for preventing and treating porcine rotavirus infection. The application finds that phosphatidylcholine (PC) has significant antiviral activity in preventing and treating PoRVA infection. Experimental results show that PC can effectively inhibit PoRVA proliferation, reduce viral load, and then relieve diarrhea symptoms caused by PoRVA infection and reduce mortality. In addition, PC can also inhibit necrotic apoptosis induced by PoRVA infection, promote repair and maintenance of intestinal barrier integrity, reduce intestinal tissue damage, and reduce the risk of secondary infection. The above results show that PC has good therapeutic potential for PoRVA infection. At the same time, PC is safe in source, has good biocompatibility, and no obvious toxic side effects are observed within a reasonable dose range, and is suitable for prevention and / or treatment of PoRVA infection, and has good application prospect and popularization value.

The present disclosure relates generally to a composition comprising a Bifidobacterium longum subsp. Infantis preserved in the form of DSM 15953 (Bifidobacterium longum subsp. Infantis), a Bifidobacterium breve (Bifidobacterium breve) preserved in the form of DSM 34541 (Bifidobacterium breve), and 2 '-fucosyllactose (2' FL). The present disclosure relates generally to a composition comprising: Bifidobacterium longum subsp. Infantis preserved in the form of DSM 34541 (Bifidobacterium breve); the use of the compositions in supporting gastrointestinal health, including promoting intestinal barrier integrity and growth of beneficial microbiota in the gastrointestinal tract, while also having beneficial effects on immune function is also part of the present disclosure.

The invention discloses an application of a rhizoma polygonati water extract in preparation of a medicine for treating acute appendicitis. The rhizoma polygonati water extract provided by the invention can be used for remarkably improving clinical symptoms of appendicitis, effectively protecting mechanical barrier integrity of intestinal mucosa and relieving inflammatory response. According to the invention, the treatment effect of the rhizoma polygonati water extract on acute appendicitis is defined for the first time, the application blank of traditional Chinese medicines in the field is filled, and a scientific basis is provided for subsequent development of similar medicines by revealing the action mechanism (such as regulation and control of tight junction proteins and inflammatory factors) of the rhizoma polygonati water extract; the method has important value in the theoretical research level and the application and popularization aspects.

The application discloses a kind of peptide functionalized cellulose derivatives targeting A beta and its preparation method and application, belong to the field of biological medicine technology.The peptide functionalized cellulose derivative obtained by connecting A beta targeting peptide with hydroxypropyl cellulose through ester bond can be combined with A beta protein through molecular recognition specificity, and self-assembly is formed into core-shell structure nanomicelle, A beta oligomer is efficiently encapsulated, A beta aggregation and fiberization are inhibited, meanwhile, the interaction between A beta and endothelial cell junction protein is blocked by anti-fouling performance, APEL effect induced by amyloid is inhibited, and the integrity of blood-brain barrier is protected.In addition, the peptide functionalized cellulose derivative has good biocompatibility, can reduce A beta plaque deposition in brain, improve the cognitive function of AD patients, and provides a new type of efficient candidate drug for the treatment of Alzheimer's disease.

In particular, the present disclosure relates to a composition comprising a Bifidobacterium animalis subsp. Lactis strain (Bifidobacterium animalis subsp. Lactis), which is preserved as DSM 15954 by the Deutsche Sammneck von Microorganism strain preservation center (Deutsche Sammneck von Microorganism strain GmbH, DSMZ), and 2 '-fucosyllactose (2' FL), and to a method for preparing the same. The present disclosure also relates to a composition that also comprises a strain of Bifidobacterium longum subsp. Infantis, which is preserved as DSM 15953, and to a method for producing the same. The present disclosure also relates to a composition that also comprises a strain of Bifidobacterium longum subsp. Infantis, which is preserved as DSM 15953. The use of the compositions in supporting gastrointestinal health, including promoting intestinal barrier integrity and growth of beneficial microbiota in the gastrointestinal tract, while also having beneficial effects on immune function is also part of the present disclosure.

This invention relates to the fields of molecular biology and biomedicine, specifically to a method and application of recombinant methods for the grass carp IL-21 optimized gene, its recombinant protein, and plasmids. The recombinant protein of this invention is a mature grass carp IL-21 peptide. The pET-28a-IL-21 recombinant expression plasmid was constructed by optimizing the IL-21 base codons, transformed into an *E. coli* (DE3) expression system, and soluble grass carp IL-21 recombinant protein was obtained by IPTG induction. Furthermore, this invention also found that the optimized gene, its recombinant protein, and plasmids can enhance the intestinal barrier integrity of grass carp, inhibit the load of pathogenic bacteria in the intestine, and repair intestinal damage caused by bacterial infection. This indicates that the grass carp IL-21 recombinant protein has a significant effect on protecting intestinal homeostasis, highlighting its promising application prospects in the research and development of prevention and treatment of bacterial enteritis in aquatic animals.

The present invention relates to a composition for use in improving the gastrointestinal microbiota, and maintaining and / or improving gut barrier integrity in an individual, the composition comprising the combination of xylooligosaccharides (XOS) and inulin. A method of preparing said composition and the use of said composition as a dietary supplement or a medicament are also disclosed.

The invention provides a carrier-free GMR hydrogel drug formed by self-assembly of natural active components glycyrrhizic acid (GA) and rosmarinic acid (RA) mediated by Mg < 2 + >, and the carrier-free GMR hydrogel drug is used for treating inflammatory bowel disease (IBD). The hydrogel medicine is simple in preparation method and good in gelation property. In animal models of ulcerative colitis and Crohn's disease, the GMR hydrogel shows remarkable prevention and treatment effects. The action mechanism of the compound is mainly derived from a cell protection effect, mitochondrial damage caused by oxidative stress can be relieved, and an NLRP3 / caspase-1 / GSDMD mediated pyroptosis pathway in intestinal epithelial cells is inhibited. Meanwhile, the hydrogel can restore the integrity of the intestinal barrier by up-regulating tight junction protein, and can play a comprehensive therapeutic effect on IBD by remodeling intestinal flora, enhancing the diversity of the flora and promoting the generation of short-chain fatty acid. In addition, the GMR hydrogel shows good biological safety and a simple and convenient preparation process, so that the hydrogel based on a natural product is expected to become an IBD treatment strategy with a wide application prospect.

This invention provides a dietary fiber-isochlorogenic acid complex, its preparation method, and its applications, relating to the field of food processing technology. The invention employs an ultrasound-assisted eutectic solvent method to extract soluble dietary fiber from the outer leaves of Chinese cabbage. The hydrogen bond donor in the eutectic solvent is lactic acid, and the hydrogen bond acceptor is one of choline chloride, glycine, and alanine. Simultaneously, this invention prepares a soluble dietary fiber-stevia isochlorogenic acid complex from the outer leaves of Chinese cabbage and investigates its protective effect on the intestinal barrier in mice. The results show that the soluble dietary fiber-stevia isochlorogenic acid complex from the outer leaves of Chinese cabbage can protect the integrity of mouse intestinal villi, reduce intestinal barrier permeability, maintain the function of mouse immune organs and the intestinal immune barrier, maintain the integrity of the intestinal mechanical barrier, improve the intestinal flora structure, and enhance humoral immunity, cellular immunity, and non-specific immune function.

The present invention relates to the use of hemichannel blockers to regulate BRB integrity and function, RPE integrity and function, tight junction integrity and function, ZO-1 internalization, gap junction internalization, and / or type IV collagen levels in a subject. The retinal pigment epithelium layer, or retinal pigment epithelium (RPE), is the pigment cell layer immediately outside the neurosensory retina that nourishes retinal photoreceptors and is firmly attached to the underlying choroid and underlying retinal photoreceptors. Increased permeability of the RPE and the blood-retinal barrier is involved in numerous diseases, disorders, and conditions.

The invention discloses oral spirulina delivery hydrogel as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The delivery hydrogel takes spirulina platensis as a raw material, and is added into network-shaped hydrogel formed by xanthan gum / konjac glucomannan / sodium alginate in a combined manner. The method is easy to implement, safe in component and high in encapsulation efficiency. The composite hydrogel has the following effects: after oral administration, the spirulina can be protected to completely pass through the stomach, the intestinal retention is prolonged, and the slow release of the medicine at the focus part is realized; according to the present invention, with the application of the polypeptide, the oxidative stress and the intestinal inflammation can be alleviated, and the expression of the tight junction protein can be significantly recovered so as to enhance the barrier integrity and limit the inflammation leakage, such that the whole body immune homeostasis conversion can be achieved, the good safety and the good compliance can be provided, and the clinical transformation potential as the systemic chronic inflammatory disease adjuvant therapy strategy can be represented; the traditional Chinese medicine composition is especially suitable for diseases with pathological characteristics of intestinal flora imbalance or immune disorder.

The application provides a method and system for predicting the damage of testicular supporting cells by vomitoxin, and relates to the technical field of testicular supporting cell damage prediction, which comprises obtaining transcriptome sequencing, immunofluorescence quantification, electron microscope ultrastructure and flow detection data; based on the theory of persistent coherence, the topological characteristics of the gene expression space are extracted and encoded into persistent bar code representation; through a neural differential equation, the bar code is fused with protein and structure data for multi-modal fusion and dynamic evolution modeling, and topological kinetic characteristics representing barrier function are obtained; combined with flow data, a cell state phase transition model is constructed by applying a cellular automaton and percolation theory to simulate the critical damage threshold of the blood-testis barrier integrity; a potential well model is constructed based on the threshold and flow data; finally, the path integral and variation method are used to calculate the damage probability of cells under different toxin concentrations. The application realizes early warning and dynamic risk prediction of testicular supporting cell damage by vomitoxin.

The invention discloses an application of intestinal Rosebaria capable of promoting intestinal bacteria to produce valeric acid and a composition of the intestinal Rosebaria in relieving ulcerative colitis, and belongs to the technical field of microorganisms. According to the intestinal Rosiberia NSP017 disclosed by the invention, the konjac glucomannan can be quickly utilized, and the composition of the intestinal Rosiberia and the konjac glucomannan can be used for improving the disease activity index and spleen enlargement of a mouse with ulcerative colitis, maintaining the integrity of an intestinal barrier, reducing the oxidative stress level, and improving the ulcerative colitis activity index and the spleen enlargement of the mouse with ulcerative colitis, so that the intestinal Rosiberia NSP017 can be used for treating ulcerative colitis. The level of a clinical diagnosis marker for colitis is remarkably reduced, the effect is superior to that of a clinical medicine mesalazine, and it is found that the intestinal Rosebawnella NSP017 can promote intestinal bacteria of mice with colitis to recover the capacity of producing valeric acid. The intestinal Rosibula sp. NSP017 and the composition of the intestinal Rosibula sp. NSP017 have very wide application prospects in the aspect of preparing foods, pharmaceutical compositions, health-care products or feed additives for relieving ulcerative colitis.

The invention discloses application of bacteroides xylanolyticum capable of promoting intestinal bacteria to produce propionic acid and a composition of the bacteroides xylanolyticum in relieving ulcerative colitis, and belongs to the technical field of microorganisms. The bacteroides xylanolyticus NSP016 disclosed by the invention can quickly utilize konjac glucomannan, and the composition of the bacteroides xylanolyticus and konjac glucomannan can improve the rise of histopathologic score of mice with ulcerative colitis, maintain the integrity of intestinal barriers, reduce the oxidative stress level and remarkably reduce the level of clinical diagnosis markers of colitis, so that the clinical diagnosis of colitis is facilitated. The effect is better than that of a clinical medicine mesalazine. The bacteroides xylanolyticum NSP016 and the composition thereof disclosed by the invention have very wide application prospects in the aspect of preparing foods, pharmaceutical compositions, health-care products or feed additives for relieving ulcerative colitis.

The present application relates to tilapia breeding technical field, especially to a kind of compound additive for enhancing the intestinal barrier function of fragile tilapia.The technical scheme: a kind of compound additive for enhancing the intestinal barrier function of fragile tilapia, including the following components by mass percentage: (A) 2%~15% of targeted physical barrier repair agent;(B) 60%~85% of etiology specific scavenger;(C) 10%~30% of intelligent time sequence delivery carrier, through the trinity design of targeted repair, etiology clearance, intelligent delivery, the pain point of single function of traditional scheme is systematically solved, it not only adjusts flora (biological barrier) by probiotic, more through targeted repair agent actively strengthens tight junction (physical barrier), and through antioxidant component and specific probiotic synergistic maintenance mucosa health (chemical and immune barrier), synchronous, synergistic enhancement to intestinal four barriers is realized, and barrier integrity is significantly improved.

The application discloses a skin corrosivity evaluation method based on an epidermal model, which comprises the following steps: providing human induced pluripotent stem cell (hiPSC) derived keratinocytes, and inoculating the keratinocytes in collagen-coated transmembrane Transwell chambers for submersion culture; detecting a trans-epithelial electrical resistance (TEER) value of the epidermal model to evaluate barrier integrity of the epidermal model; taking the epidermal model with barrier function confirmed by the TEER value detection, washing the epidermal model with a detergent, and then dividing the epidermal model into a 3 min exposure group and a 60 min exposure group; after the exposure ends, the epidermal model is sufficiently flushed with the detergent, and then a thiazolyl blue colorimetric (MTT) method is used to detect cell viability; and according to a cell viability percentage (Vc) of a test object at two exposure time points of 3 min and 60 min, it is determined whether the test object has skin corrosivity; the method can better simulate an in-vitro skin barrier and a corrosion reaction process, and can be used for in-vitro evaluation of skin corrosivity of cosmetics, drugs and chemicals.

The application belongs to the technical field of microorganisms, and particularly relates to a high uric acid relieving Paracasei postbiotic and application thereof. Lacticaseibacillus paracasei The Paracasei (Probio-37) has a preservation number of CGMCC No. 18638. The postbiotic prepared by the strain stimulates the intestinal uric acid excretion pathway through the "gut-kidney axis", reduces the SUA level, adjusts the immune homeostasis, inhibits excessive inflammatory response, enhances the intestinal barrier integrity, effectively relieves local inflammatory response caused by urate crystals, and thus improves clinical symptoms such as joint swelling and pain, and has obvious therapeutic effect on hyperuricemia.