The invention establishes a myocardial injury model for a zebra fish, and provides a method for evaluating the toxicity of a myocardial injury inducing agent and the efficacy of a myocardial injury treating agent by using the zebra fish. The invention provides a brand-new medicine evaluating model and a brand-new medicine evaluating method; the medicine evaluating model and the medicine evaluating method have the advantages of reliability, fastness, high efficiency, cheapness, high cost performance and the like, can be used for evaluating the efficacy of disclosed medicines, can be also used for screening undisclosed medicines adopting brand-new compound structures, and are wide in application and high in significance.