104 results about "Injury model" patented technology

The invention relates to extraction of an active ingredient exosome from a human umbilical cordmesenchymal stem cell and application thereof in preparation of medicaments for treating liver and kidney injury and skin diseases. The human umbilical cordmesenchymal stem cell-derived exosome is prepared by the following steps of: (1) culturing a human umbilical cordmesenchymal stem cell; (2) preparing a cordmesenchymal stem cell conditioned medium; and (3) separating and purifying the exosome. The experiments for infusing the human umbilical cordmesenchymal stem cell exosome serving as a medicament into a mice acute liver injury model, a rat kidney injury model and a rat skin scald model show that the medicament can improve the liver function, the kidney function and the skin condition, promote the pathological recovery of the liver, the kidney and the skin, reduce the death rate, fulfill the aims of blocking and reversing the development of the liver and kidney injury and open up a new approach for clinical treating of the liver and kidney injury.

The invention aims to provide an active polypeptide separated from an anchovy fermentation liquid. The molecular weight of the nerve cell injury resistant polypeptide obtained by the invention is 2,181Da; and the polypeptide is composed of 11 amino acids such as aspartic acid, threonine, glutamic acid, glycine, alanine, isoleucine, tyrosine, phenylalanine, histidine, lysine, arginine and the like, and is a novel active polypeptide with activity. According to the polypeptide obtained by the invention, in a glutamine nerve cell injury model, the addition of the active polypeptide provided by the invention can obviously increase the survival rate of PC12 nerve cells; and when the final concentration is 50 mug / mL, the cell survival rate is increased by 26.4% in comparison with an injury group, thereby effectively relieving glutamine-induced nerve cell injury. Besides, the cell mechanism study on the nerve cell protective action of the polypeptide discovers that the addition of the active polypeptide obviously increases the intracellular total oxidation resistance and has a certain promoting action on increase of SOD (superoxide dismutase) activity.

The invention discloses a method for preparing a collagen material for repairing peripheral nerve injury. The method comprises the following step: co-incubating a ciliary neurotrophic factor with a collagen binding domain (CBD-CNTF), a basic fibroblast growth factor with a collagen binding domain (CBD-bFGF) and an orderly collagen material to obtain a collagen material for repairing the peripheral nerve injury. According to the preparation method, two factors CBD-CNTF and CBD-bFGF are simultaneously crosslinked to the orderly collagen scaffold material, and the action of the double-factor peripheral nerve injury repairing collagen material in long-cross-section nerve injury repairing can be evaluated by establishing an animal peripheral nerve-facial nerve long cross-section injury model. Results prove that compared with single factor CBD-CNTF or CBD-bFGF functional collagen scaffold material, the double-factor peripheral nerve injury repairing collagen material can be used for better promoting regeneration of facial nerves and restoration of functions, and has a more important realistic guiding magnificence to application of clinical peripheral nerve injury repairing in the future.

The invention discloses an injury making device for deceleration injury experimental research and a method for making a deceleration injury mould. A space for the pulley falling is existed between an upper supporting seat and a substrate. The space is internally and vertically provided with a guided way. The pulley is arranged between the upper supporting seat and the substrate and is matched with the guided way by a rolling device in a rolling way. The substrate is provided with a chopping block and a device used for buffering and deceleration. An animal is fixed on the pulley. A bracket is used for holding the position of the animal needing to be injured. The pulley is caused to freely fall along the guided way so that the position of the animal needing to be injured collides with the chopping chock. The device used for buffering and deceleration controls deceleration so as to form the deceleration damage of the position of the animal needing to be injury, thus obtaining the mould of the deceleration injury. The invention overcomes the shortcomings of the existing injury making device for deceleration injury and the method for making the deceleration injury mould, is economical and scientific and can be widely used in the fundamental research of medicines such as traffic medicine, physical exercise medicine, aviation and aerospace and etc.

The invention discloses a multisource data mining-based vehicle intelligent driving system validity evaluation method. The method comprises the steps of 1) obtaining a to-be-evaluated vehicle model, a random traffic scene model and a passenger injury model through multisource data mining; 2) based on the random traffic scene model and the vehicle model, simulating a specific scene of an accident; 3) simulating the scene by utilizing accident reconstruction software, and outputting a post-collision vehicle state; 4) in combination with the passenger injury model, calculating a passenger injury risk and a unit mileage risk after the accident; and 5) replacing the to-be-evaluated vehicle model with a conventional model, and repeating the steps 1) to 4) to obtain unit mileage and passenger injury risks of a vehicle free of a to-be-evaluated system; and then comparing injury results under the conditions that the to-be-evaluated system exists and does not exist, thereby obtaining the validity of the system. An evaluation platform depends on multisource data and known software, can realize evaluation of multiple intelligent driving systems, is low in running cost and high in speed, and has relatively good universal applicability.

The invention establishes a myocardial injury model for a zebra fish, and provides a method for evaluating the toxicity of a myocardial injury inducing agent and the efficacy of a myocardial injury treating agent by using the zebra fish. The invention provides a brand-new medicine evaluating model and a brand-new medicine evaluating method; the medicine evaluating model and the medicine evaluating method have the advantages of reliability, fastness, high efficiency, cheapness, high cost performance and the like, can be used for evaluating the efficacy of disclosed medicines, can be also used for screening undisclosed medicines adopting brand-new compound structures, and are wide in application and high in significance.

The invention relates to an estimation and self-healing control method for a blade injury of a multi-rotor unmanned aerial vehicle autopilot. The estimation and self-healing control method comprises the steps of: firstly, calculating a moment acted on a machine body by an instruction output by an autopilot control system under a non-fault condition and a mathematical model between a force and moment which are acted on the machine body; then constructing a machine body attitude control system state equation, and estimating a moment disturbance variable generated for the machine body after the blade injury by adopting a time domain disturbance observer; then inverting and resolving a parameter estimated value of a blade injury model according to an inverse model of the blade injury mathematical model; and finally, according to the parameter estimated value of the blade injury model, designing a control distribution reconstruction matrix to implement control distribution reconstruction ofthe autopilot. Compared with the prior art, the estimation and self-healing control method for the blade injury of the multi-rotor unmanned aerial vehicle autopilot, which is disclosed by the invention, has the following advantages that a blade injury degree can be estimated online; and by reconstructing a control object mathematical model online, control self-healing of the multi-rotor unmannedaerial vehicle autopilot under the blade injury is implemented, so as to ensure safe and stable flight of an unmanned aerial vehicle under the blade injury condition.

The invention discloses a device for manufacturing craniocerebral explosion injury models for small animals, which comprises a supporting plate, four upright posts arranged on the surface of the supporting plate, longitudinal guide rails, longitudinal sliding blocks, transverse guide rails, transverse sliding blocks, a vertical sliding block and a charging cabin, wherein the longitudinal guide rails are arranged at the top ends of the two upright posts adjacent with each other on the longitudinal direction; the longitudinal sliding block is movably arranged on each longitudinal guide rail; the two transverse guide rails are fixedly arranged between the two longitudinal guide rails; the vertical sliding block is arranged between the two transverse guide rails; the charging cabin is arranged in the vertical sliding block; and the bottom end of the charging cabin penetrates through the bottom surface of the vertical sliding block. Controllable precise explosion on any specific part of a testing animal can be realized through the free adjustment in the longitudinal, transverse and vertical directions so as to obtain the animal explosion injury model through shock waves.

The invention discloses an intelligent passenger injury monitoring platform and method based on the Internet of vehicles. After a vehicle collision accident, a passenger injury model is established through cloud computing based on the information collected by a passenger information collection system, the calculation result is corrected based on previous data called based on big data, and the accurate passenger injury information is obtained and transmitted to a vehicle remote monitoring terminal, a hospital rescue information management platform and a traffic management information managementplatform. When a passenger has no obvious damage, the passenger is reminded by the vehicle remote monitoring terminal to seek medical treatment in time, when the passenger is seriously injured, the passenger injury information is sent by a workstation to the hospital rescue information management platform, nearby hospitals and rescue workers are reminded to prepare for the rescue, the collision accident information is sent by the workstation to the traffic management information management platform, and the traffic police is informed to deal with the traffic accident.

The invention discloses application of soracan gum in inhibiting skin injury of ultraviolet ray, and relates to application of soracan gum with different mass concentrations in protecting skin from injury due to ultraviolet ray UVA. A cell model adopted in the invention is an ultraviolet ray UVA induced cell injury model, wherein the dosage of soracan gum is 0.625-5 mu g / mu l; a mouse model adopted in the invention is an ultraviolet ray UVA induced mouse skin injury model, and the dosage of the soracan gum is 200mu l of 1 percent solution, the soracan gum can be used for improving the cell survival rate of cells in ultraviolet radiation, reducing inflammation factors in mouse skin and improving gene expression of synthetic collagen, so that skin injury caused by ultraviolet ray UVA can be inhibited. Cell and animal experiments prove that soracan gum has a remarkable effect of inhibiting skin injury of ultraviolet ray, so that soracan gum can be used in processes of preventing and repairing skin injury due to ultraviolet ray UVA, and can be used for preparing anti-ultraviolet radiation injury medicines and anti-ultraviolet radiation cosmetics.

The invention relates to a preparation method and application of a new compound in sabia parviflora. Pharmacological research indicates that the referred new compound can lower the contents of ALT and AST in CC14 induced acute hepatic injury model mice, D-amino galactose induced acute hepatic injury model mice and ethanol induced hepatic injury model mice, lower hepatic tissue MDA content, and enhance liver SOD activity, and has an obvious protection effect on hepatic injury. The new compound is expected to be developed as a medicine or healthcare product with a hepatoprotective effect.

The invention relates to the fields of medical treatment evaluation and detection technology, and in particular relates to a method for preparing an intestinal mucosa injury animal model and applications of the prepared intestinal mucosa injury animal model. According to the scheme, the medicine L-MSO having good water solubility and realizing convenient and simple gavaging operation is adopted, the mouse intestinal mucosa injury is conveniently and stably induced, and meanwhile, the corresponding tissue pathological change and inflammatory factor change are caused. For the preparation method provided by the invention, the intestinal mucosa injury animal model is prepared from the metabolism injury direction obviously different from the molding direction of immune injury inducing in the prior art, particularly, the low-concentration L-MSO is adopted for preparing the common injured model, wherein the intestinal mucosa of the laboratory mouse has the mild-moderate injury, the high-concentration L-MSO is adopted for preparing the seriously injured model, wherein the intestinal mucosa of the laboratory mouse has the severe injury; the prepared intestinal mucosa injury model has the significant values for the research and practice of inflammatory bowel diseases.

The invention discloses an application of a morindae officinalis extract and morindae officinalis oligosaccharide pentasaccharide to preparation of a drug for treating myocardial ischemia and / or reperfusion injury and promoting therapeutic angiogenesis. The myocardial ischemia and reperfusion injury belong to secondary injury brought to an organism when the hemoperfusion of myocardium is stopped or poor, i.e., after the myocardium is subjected to ischemia, hypoxia injury, circulation reinstitution and blood supply recovery. The morindae officinalis extract and the morindae officinalis oligosaccharide pentasaccharide both play a role in protecting an in-vivo rat myocardial ischemia reperfusion injury model and an in-vitro purification cultured neonatal rat myocardial cell hypoxia / reoxygenation injury model, and can be used for remarkably reducing the myocardial infarction area and the incidence rate of reperfusion arrhythmias, effectively protecting the form of a myocardial cell and relieving the injury of hypoxia / reoxygenation to the myocardial cell. The therapeutic angiogenesis promotion means that the aims of recovering the blood supply of ischemic myocardium and improving the heart function are achieved through increasing the functional coronary artery branch or side branch under the irritation actions of some methods, including the therapeutic actions of some drugs.

The invention belongs to the technical field of biological materials and nerve injury repairing, and particularly relates to a decellularized epineurium catheter and a preparation method thereof as well as application to repairing of a peripheral nerve injury. The biological characteristic of the decellularized epineurium catheter for a pig is researched from such aspects as the porosity, the swelling rate, the in vitro degradation rate and the cytotoxicity test, and the immunogenicity of the catheter is also researched. According to the decellularized epineurium catheter and the preparation method thereof, a rat sciatic nerve transversal injury model is built; after being subjected to microsurgical suturing, a nerve stump line is covered with the decellularized epineurium catheter so as to research the effects of promoting regeneration of nerves, preventing nerve adhesion and formation of nerve tumors. The invention provides the decellularized epineurium catheter which can effectively reduce surrounding nerve adhesion and reduce the occurrence rate of the nerve tumors, and a novel repairing material is provided for repairing of war traumatic surrounding nerve injuries and treatment of causalgia caused by the nerve injuries.

The invention discloses a novel saponin compound with a liver protection effect. Chemical components of liquorice are symmetrically and deeply researched, a novel triterpene saponin compound is obtained by separating from liquorice root according to spectrum and mass spectrometric data analysis. Pharmacological experiment research indicates that the triterpene saponin compound provided by the invention can be used for remarkably lowering the serum ALT (alanine aminotransferase) and AST (asparate aminotransferase) activities and the hepatic tissue MDA (methylene dioxyamphetamine) content of CC14-caused hepatic injury model mice, alcohol-caused hepatic injury model mice and D-galactosamine-caused hepatic injury model mice, can be used for strengthening the liver SOD (superoxide dismutase) activity, and has an obvious protective effect on hepatic injury; toxicity test research indicates that the novel saponin compound with the liver protection effect does not have remarkable toxicity, and is expected to be developed into a novel medicine or a healthcare product with a liver protection effect.

The invention discloses an application of a Morindae officinalis extract product and Morindae officinalis oligosaccharide 6 glycan in the preparation of medicines for resisting myocardial ischemia and / or reperfusion injury and promoting therapeutic angiogenesis. The myocardial ischemia and reperfusion injury are body injuries caused by the afresh circulation establishment and the blood supply recovery during the stopping or abnormality of the myocardial blood perfusion, like ischemic and anoxic injuries. The Morindae officinalis oligosaccharide 6 glycan has a protect effect on an in-vivo rat myocardial ischemia reperfusion injury model and an in-vitro purify-cultured mouse myocardial cell reoxygenation injury model, can substantially reduces the myocardial infarction area, can mitigate the reperfusion arrhythmia, can effectively protect the form of myocardial cells, and can mitigate the injuries of anoxic reoxygenation to the myocardial cells. The therapeutic angiogenesis promotion is characterized in that the ischemic myocardial blood supply recovery and the cardiac function improvement are realized by increasing the functional coronary arterial tree or coronary collateral through some irradiations comprising the treatment effects of some medicines.

The invention discloses a tilapia oxidative stress liver injury model and a construction method and application thereof. The method is to construct the damage model by injecting H2O2. The advantages are that: (1) a tilapia source used in the method disclosed by the invention is the same, the culture environment is controllable, and thus the repeatability is high; (2) the method disclosed by the invention applies the H2O2 to construct the fish liver injury model for the first time, and the ideal tilapia experimental model capable of being used for studying the pathogenesis of liver disease andscreening related therapeutic drugs is obtained.

The invention relates to application of a lecithin superoxide dismutase composite in preparation of medicines for treating and / or preventing cardiac ischemic injury, wherein the lecithin superoxide dismutase composite is dipolymer combined with different molecule numbers of lecithin, and the concrete method comprises the following steps of: establishing a cardiac ischemic injury animal model; preparing a cardiac injury induced model for verifying a function of the cardiac ischemic injury animal model in cardiac protection treatment under the asepsis condition, spreading sacculus, blocking the sacculus for a long time and loosening the sacculus through injecting a contrast agent into the small pig coronary artery left anterior descending branch balloon implant so as to respectively establish a myocardial infarction model and a myocardial injury model through blood liquid infusion; and injecting the lecithin superoxide dismutase composite containing different molecule numbers of lecithin into the animal model so as to observe a new function of treating cardiac injury.

The invention discloses a tilapia mossambica precision-cut liver slice injury model and a construction method thereof. The method adopts cyclophosphamide to induce tilapia mossambica precision-cut liver slices, and the concentration of the cyclophosphamide is 25mM or below. Compared with the prior art, the method disclosed by the invention has the following advantages: (1) the cyclophosphamide-induced tilapia mossambica precision-cut liver slices are adopted for the first time to construct an injury model, and a basis is provided for researching a fish liver injury mechanism and screening and developing liver protection drugs; and (2) a tilapia mossambica precision-cut liver slice is adopted as an induction object of the method, the in-vivo state is simulated to the maximum extent, and the method is a more reliable model system for researching liver metabolism and in-vitro induction toxicity.

The invention discloses functions and application of SHPS1 in treatment of post-vascular injury restenosis, belonging to the field of functions and applications of genes. SHPS1 gene knockout mice and wild type mice are taken as experimental subjects, and due to a vascular injury model, the mouse neointima formation, vascular wall cell proliferation level and smooth muscle cell phenotype transform are detected. The result proves that SHPS1 gene knockout can obviously promote neointima neogenesis and cell proliferation and promote transformation of smooth muscle cells from a shrinkage manner to a synthetic manner. Thus the functions of SHPS1 in post-vascular injury restenosis are realized, which mainly shows that SHPS1 has the functions of inhibiting neointima formation and cell proliferation and inhibiting smooth muscle cell phenotype transformation. Aiming at the functions of the SHPS1, the SHPS1 can be used for preparing medicines and arterial stents for preventing, relieving and / or treating the post-vascular injury restenosis.

The invention relates to a construction method of a lipopolysaccharide induced fish multi-organ injury model, and belongs to the technical field of aquaculture. The method comprises the following steps of firstly, selecting healthy cultured fishes as objects for establishing an organ injury model; preparing lipopolysaccharide into a lipopolysaccharide solution; carrying out intraperitoneal injection and intragastric administration by adopting the lipopolysaccharide solution to obtain the lipopolysaccharide induced fish organ injury model; and carrying out detection. The construction method ofthe lipopolysaccharide induced fish multi-organ injury model has the minimum influence on the non-specific immunity of the fishes, but the organ injury pathological manifestation of the fishes is mostsuitable, so that the model is stable and reliable, and the reproducibility is good.

The invention discloses a method for constructing a blood-spinal cord barrier (BSCB) oxygen-glucose deprivation / reoxygenation (OGD / R) injury model and an application thereof. Spinal cord derived microvascular endothelial cells were cultured in the upper chamber and mixed glial cells were cultured in the bottom chamber of the transwell culture system to construct the in vitro BSCB model. Microvascular endothelial cells in the upper chamber of transwell and mixed glial cells in the lower chamber of transwell interact with each other by secreting cytokines to form molecular membranes with good barrier function. OGD / R can effectively simulate the injury of BSCB induced by ischemia / reperfusion in vivo, and can more directly and accurately understand the changes of structure and function of BSCBafter ischemia / reperfusion injury. The results of the study have a high reference value, thus providing an effective basis for clinical study of BSCB.

The invention provides a determination method for an indoor rapid freeze-thaw cycle and natural freeze-thaw cycle relation of concrete. The method comprises the following steps: step 1: obtaining a design matching ratio and a strength grade of hydraulic concrete of a concrete water conservancy; step 2: deigning and expanding N types of freeze-thaw tests of different freeze-thaw cycles at the lowest temperature, wherein N is greater than or equal to 3, so as to obtain concrete degradation testing data under different freeze-thaw times; step 3: establishing a concrete freeze-thaw injury model ofa freeze-thaw cycle temperature procedure; step 4: establishing a relation between an indoor rapid freeze-thaw cycle and a natural freeze-thaw cycle of the concrete. According to the determination method for the indoor rapid freeze-thaw cycle and natural freeze-thaw cycle relation of the concrete, provided by the invention, the problem of an existing method that influences, caused by the freeze-thaw cycle temperature procedure, on concrete freeze-thaw injuries can be solved, and natural freeze-thaw degradation of an on-site water conservancy can be quantitatively guided by indoor rapid freezing and thawing.

The invention belongs to the technical field of animal model building, and particularly relates to a fine particle inhaling device for building an animal injury model. The fine particle inhaling device for building the animal injury model comprises a dust smoke generator, a dust inhaling device and a dust smoke recovering device, wherein the inhaling device can form stable fine particle inhaling environment with controllable concentration so as to build the animal injury model for simulating a dust-haze environment; meanwhile, the inhaling device can achieve the goal of performing stable fine particle inhaling on various animals at the same time in one step; various particles can even be used for realizing the absorption contamination. The inhaling device has the advantages that the structure is simple; the operation is easy; the animal absorption contamination effect is more stable and more uniform; the recovering device is installed, so that the particle recovery can be realized; the secondary pollution on environment and the injury on the experiment personnel are avoided.

The invention provides an application of an ErbB receptor agonist in preparing medicines for treating ischemic cerebrovascular diseases By using neuronal injury models in vivo and in vitro as a research platform, the protection function of NRG1 to neuronal cell injuries and experimental cerebral ischemia are discovered, simultaneously the possible molecular mechanism of NRG1 is fully researched, and the cerebral protection effect by regulating and controlling NRG1-ErbB4 associated signal pathway after ischemic cerebral ischemia is fully argued, thus the study of NRG1 and associated ErbB4 agonist open up wide prospects for developing novel medicines for protecting cerebral neurocyte and controlling ischemic cerebrovascular diseases.

The invention relates to an Ovateleaf Holly Bark extract product having a hepatic injury prevention effect, and an application thereof. Ovateleaf Holly Bark is dried bark or velamen of an Aquifoliaceae plant Ilex roundda Thunb.. The active extract product is prepared on the basis of researches of the traditional efficacies of Ovateleaf Holly Bark. The Ovateleaf Holly Bark extract product can substantially reduce the activity of ALT and AST in the serum of CCl4 / alcohol caused hepatic injury model mice and the content of MDA in hepatic tissues of the mice, and enhances the SOD activity of livers, so the active extract has an obvious hepatic protection effect on chemical hepatic injuries and alcoholic hepatic injuries.

The invention discloses applications of dendrobium nobile lindl alkaloids (DNLA) in medicines prepared for treating ischemic brain damage and a product thereof. The DNLA is lipophilic total alkaloids composition which is extracted from the dendrobium nobile lindl. Compared with the prior art, the invention aims at the nosogenesis of stroke, studies the protection function of the DNLA on oxygen-glucose deprivation injury model of primary cortical neurons and acute cerebral ischemia model of rats, analyzes the mechanism, further makes clear the pharmacological action and mechanism action of the DNLA for treating cerebral ischemia, and provides pharmacological basis for the development and applications of the dendrobium nobile lindl medical material.

The invention relates to a device for building of a rat spinal cord explosion injury model in the field of medical experiment devices. The device comprises a bottom plate (1), a supporting column (2) fixed on the bottom plate (1), an explosion source supporting rod (3) arranged on the supporting column (2) and an animal box (5) arranged on the supporting column (2) through an animal box supporting rod (4). The bottom plate (1) is provided with grooves (7), a seam (8) is formed in the grooves (7), the bottom of the seam (8) is provided with a sensor probe, and the box bottom (15) of the animal box (5) is provided with grooves (7) and a seam (8) which corresponding to those of the bottom plate (1). The device overcomes the defects that an existing spinal cord explosion injury model can not achieve precise explosion of a target portion and is poor in experiment accuracy and control property. The device for the building of the rat spinal cord explosion injury model is simple to operate, good in practical control, and capable of achieving the precise explosion of an injury of the target portion.

The invention discloses a method for making an aortic endothelial cell injury model by utilizing mice and a detection method. The method for making the aortic endothelial cell injury model disclosed by the invention comprises the following steps of: (1), cutting femoral artery surface skin of mice, and separating femoral artery from a femoral artery branch; (2), shearing a small hole on the femoral artery branch, and inserting a spiral stainless steel wire in a femoral artery trunk through the sheared hole of the femoral artery branch; (3), drawing out the spiral stainless steel wire after staying for 1-3 min, ligating the femoral artery branch, and suturing skin; and (4), making an aortic section slice from injured femoral artery after 3-5 weeks, and then, making a specimen. According to the invention, mice can be used as experimental objects for mechanical injury of aortic endothelial cells; in addition, the purpose of researching the reaction degree of the aortic endothelial cells to injury can be achieved with the help of the detection process of the aortic endothelial cells.

The invention discloses a building method of a zebra fish intervertebral disc injury model. The method comprises DNA (deoxyribonucleic acid) polymerase chain type reaction, and is characterized in that the primers and the sequences used in the DNA polymerase chain type reaction as shown as follows: a positive primer: 5'-GTCGACGTACTGTATCTGTGCTGATTCAC-3'; a reverse primer: 5'-GACCAGAAGAGATAATTCAAACGT-3'. The building method aims at solving the technical problems to provide the building method of the zebra fish intervertebral disc injury model for providing the theoretical basis for the intervertebral disc degeneration study.