20 results about "Injury model" patented technology

The invention provides a knee joint personalized rehabilitation scheme generation method and system based on digital twinning, and the method comprises the steps: obtaining the multi-dimensional feature data of a patient to construct a patient rehabilitation data set, constructing a knee joint injury knowledge graph based on the patient rehabilitation data set, constructing a knee joint minimal injury model based on the knee joint injury knowledge graph, and generating a knee joint personalized rehabilitation scheme. Functional weak links and injury degrees of the patient during rehabilitation training are accurately recognized, a data basis is provided for subsequently formulating a personalized scheme for the condition of the patient, emotion extraction is performed on voice feedback data in a patient rehabilitation data set, the patient emotion index is obtained, rehabilitation efficiency diagnosis is performed according to the patient emotion index, and the rehabilitation efficiency of the patient is improved. According to the method, the initial rehabilitation scheme of the knee joint is generated, the initial rehabilitation scheme of the knee joint is subjected to efficiency evaluation and scheme optimization, and the rehabilitation scheme of the knee joint is generated, so that the rehabilitation scheme is dynamically adjusted according to the personalized requirements of the patient while the personalized requirements of the patient are met, and the accuracy and efficiency of the generated rehabilitation scheme are improved.

The invention relates to an establishment method of a high-fat induced intestinal organ injury model and an injury intervention method for the model. The establishment method comprises the following steps: step A, preparation: preparing colon cells, PBMC cells and an organ-like culture chip for later use; step B, colon barrier construction: inoculating colon cells to the surface of a barrier membrane on the lower side of a culture chip by using an organoid culture chip to obtain a colon barrier model; step C, constructing a co-culture model, and inoculating PBMC cells to the basal side of the colon barrier on the basis of the colon barrier to obtain a human colon barrier-PBMC co-culture model; d, constructing a damage model, and performing damage treatment on the co-culture model by using a treating fluid to obtain the damage model; according to the invention, the barrier function organ-like chip is used, a human intestinal cell-PBMC co-culture model is formed by utilizing human intestinal organs and PBMC cells, and OXLDL / palmitic acid can play a role in an injured intestinal organ barrier model and form a high-fat induced intestinal organ injury model through the prepared injury treating fluid.

The invention relates to the technical field of mouse spinal cord modeling, in particular to a mouse spinal cord clamp injury model construction system and method, the system comprises a mechanical arm, a clamp, a data processing and control module and an optical imaging and measuring module, at least one clamping surface of the clamp is provided with a pressure sensor, and the pressure sensor is connected with the mechanical arm. A displacement sensor is arranged on each clamping arm of the clamp; the mechanical arm is connected with the clamp and used for driving the clamp to move, open and close; the pressure sensor, the displacement sensor, the mechanical arm and the optical imaging and measuring module are all in signal connection with the data processing and control module. The defect that in the prior art, due to individual differences of mice, the damage degrees of models under the same pressure are different, and the reliability of experimental results is low is overcome; clamping force can be adjusted according to the diameter of the spinal cord of a mouse, it is ensured that the spinal cords of different sizes are extruded to the same degree, accordingly, injuries with the highly consistent severity degree are generated, and the repeatability and statistical efficacy of experiments can be improved.

The present application relates to the technical field of medical service support, and particularly relates to a personnel vulnerability analysis method for precise medical service support, comprising: target analysis: dividing a human body into parts according to human body functions or professional functions; establishing a human body injury spectrum based on each human body part; constructing an injury model: the plurality of injury models include a projectile injury model, a thermal injury model, a shock wave injury model and an impact vibration injury model; constructing a mapping relationship under various working conditions: calculating physical injury and functional injury under a preset strike working condition based on the injury model; obtaining vulnerability distribution law: performing personnel vulnerability analysis according to the human body injury spectrum and the injury model to obtain the vulnerability distribution law and statistical results under the preset strike working condition. The present application can obtain accurate medical service support data, so that the medical service support data is more stable, scientific, reasonable and accurate.

The invention discloses a carbon tetrachloride damage model construction and nutrition intervention evaluation method based on a three-dimensional liver chip, and belongs to the technical field of microfluidics and biological medicines. According to the method, an in-vitro bionic liver tissue model is constructed by utilizing a three-dimensional liver chip, liver cell injury is induced by perfusing carbon tetrachloride, on the basis, substances such as algae-derived extracellular vesicles are added for repairing intervention, and meanwhile, contrastive analysis is performed with a two-dimensional plate culture system. Results show that the secretion level and the function maintenance time of albumin, urea and the like of liver cells in a liver chip model are superior to those of two-dimensional culture, and after nutrient intervention is carried out, liver injury indexes such as ALT, AST and the like in the chip model are remarkably reduced, and the cell activity and the metabolic function are obviously recovered. The model can be used for evaluating the curative effect of nutrient substances on chemical liver injury, overcomes animal experiment species difference and two-dimensional culture limitation, provides an efficient and sensitive in-vitro research platform for medicine and nutrition function screening, and has wide application prospects and industrialization potential.

The invention provides a method for evaluating the repair activity of a test substance, which comprises the following steps: constructing a 3D epidermis injury model, treating the injured epidermis by using the test substance, detecting the secretion amounts of IL-8 and MCP-1 in the epidermis before and after treatment, and evaluating the repair activity of the test substance based on the secretion amounts of IL-8 and MCP-1, the evaluation result obtained by the method is consistent with the evaluation result of efficacy verification performed by using a human body in trend, the blank of in-vitro evaluation of the injury repair efficacy is filled, a feasible scheme is provided for animal replacement experiments, and a reference basis is provided for further research of skin care product development and efficacy verification.

The invention discloses a construction method and application of an animal skin injury model, and relates to the technical field of biomedicine. According to the construction method, based on the microneedle technology, through the integrated design of parameter quantification control, operation standardization, injury form customization and recovery cycle regulation and control, precision, diversification and standardization of the animal skin injury model are achieved. According to the method, unified operation specifications are formulated by relying on a quantifiable parameter system of a sterile fixed microneedle and combining animal skin characteristics, it is ensured that the damage depth / area deviation of models of the same batch and different batches is controllable, and stable construction of the animal standardized skin damage model is achieved; accurate control of'epidermis-dermis' layered gradient injury is realized through accurate matching of microneedle parameters and skin levels; according to the invention, polymorphic injury customization is also realized: diversified injury types are generated through combination of an operation mode and microneedle parameters; and key design of continuous damage and recovery period regulation and control.

The application discloses a kind of based on AI mouse cranium brain thoracic and abdominal complex injury dynamic model preparation method and system, belong to the technical field of biomechanics injury.The method comprises: impact is applied to mouse skull, the impact is conducted along organ synergistic conduction chain, to induce the cranium brain thoracic and abdominal complex injury of mouse;In the process of applying impact, multi-modal physiological data are synchronously collected;The multi-modal physiological data collected are input to AI analysis model, the injury mechanism of the cranium brain thoracic and abdominal complex injury is analyzed, and dynamic model is established based on analysis result.The technical scheme of the present application realizes the high simulation modeling of multiple organ cascade injury by constructing organ synergistic conduction chain from cranium to thoracic cavity;Combined with multi-modal physiological data synchronous acquisition and AI analysis model, the injury timing characteristics can be accurately extracted, the causal path of local injury triggering system response is established, and the accuracy and practicality of complex injury mechanism prediction are improved.

The invention discloses a construction method and application of a zebra fish tail fin ischemia injury model with both inflammatory response and regeneration capacities, rose bengal is injected into the abdominal cavity of a zebra fish, then the zebra fish tail fin ischemia injury model is constructed by performing local fluorescence irradiation on the tail fin of the zebra fish and performing photochemical induced injury, the operation is simple and convenient, and the construction method is suitable for large-scale popularization and application of the zebra fish tail fin ischemia injury model. And the death rate of the zebrafish in one day after modeling of the zebrafish is very low (only 5.3%), and the method has good repeatability and operability. The photochemically induced caudal fin injury model has pathological characteristics closer to clinical tissue injury, can simulate inflammation and regeneration processes at the same time, and has dual application potentials of drug screening and mechanism research.

The application belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition for protecting gastric mucosa and application. The raw material of the traditional Chinese medicine composition is Corydalis and Fructus Meliae, and the preparation method comprises the following steps: firstly, Corydalis is extracted with water, and filtered to obtain Corydalis extract and Corydalis residue; then, Fructus Meliae and the Corydalis residue are mixed with water, and enzyme is added for enzymolysis to obtain an enzymolysis solution; finally, the Corydalis extract and the enzymolysis solution are mixed, sterilized, fermented with probiotic bacteria liquid, and filtered to obtain the traditional Chinese medicine composition. The composition prepared by the preparation method has a significantly better protection effect on gastric mucosa than other methods due to the reasonable combination of Fructus Meliae and Corydalis, can significantly reduce the gastric mucosa lesion degree of experimental gastric mucosa injury model animals, and improve the repair ability of gastric mucosa.

The invention discloses an artificial intelligence dynamic evaluation mouse multiple injury model construction method and system, and belongs to the technical field of injury models. The method comprises the steps that controlled impact is applied to the brain of a mouse to cause brain injury, and controlled trauma is introduced into the thoracic cavity and the abdominal cavity to simulate thoracic cavity and abdominal cavity injury; physiological parameters of the injured mouse are monitored in real time through a sensing device, and physiological data reflecting craniocerebral, thoracic and abdominal injury influences are collected; inputting the physiological data into an artificial intelligence algorithm model, evaluating the injury severity of the injured mouse in real time and generating a corresponding evaluation result; the evaluation result is used for grading the severity of the injury, and a corresponding mouse craniocerebral combined thoracic and abdominal multiple injury model is established according to the grading result. According to the technical scheme, accurate simulation and grading evaluation of multi-part injuries of the mouse are achieved, and multi-injury mechanism exploration and intervention strategy effect verification are effectively supported through multi-modal physiological parameter real-time monitoring and artificial intelligence dynamic analysis.

The invention specifically discloses a platelet injury assessment method and device for a hemodialysis catheter, a medium and a program product, and the method comprises the steps: obtaining the actual structural characteristics and clinical standard size of the hemodialysis catheter, and carrying out the geometric modeling of the hemodialysis catheter; performing high-precision grid division and hemodynamic analog simulation on the geometric model of the hemodialysis catheter; setting boundary conditions of each fluid domain, and establishing a shear stress and vorticity coupled damage evaluation mechanism by introducing vorticity as coupling characterization of local shear stress and eddy current effect; calculating the vortex intensity and shear stress of the blood of the blood flow velocity field through the damage assessment mechanism, and constructing a blood damage model coupled with a time accumulation effect; particle statistical data are obtained through particle tracking in the hemodialysis catheter, and platelet injury calculation is carried out through a blood injury model. The accuracy of platelet injury estimation of the hemodialysis catheter can be improved.

The invention provides a drug screening method and device based on a blood-brain barrier injury model and a medium, and the method comprises the steps: collecting experimental metadata and biological sample measurement data of the blood-brain barrier injury model, the experimental metadata comprises an identifier of a candidate drug, an animal identifier, a body weight, an Evans blue injection volume, circulation time, perfusion time and a sampling position, and the biological sample measurement data comprises a brain tissue weight and a fluorescence intensity measurement value; converting the biological sample measurement data into a quantitative index of blood brain barrier leakage, wherein the quantitative index is the Evans blue leakage amount per gram of brain tissue; and generating a prediction effect of the candidate drugs according to experimental metadata and the quantitative indexes. The blood-brain barrier leakage can be rapidly and accurately evaluated through Evans blue staining and fluorescent quantitative technologies.

The invention belongs to the field of experimental instruments, and particularly relates to a portable controllable spinal cord smashing injury modeling instrument and a use method thereof. A height adjusting device is arranged on the operation table, one side of the height adjusting device is connected with the first end of a main frame, a smashing rod slidably penetrates through the second end of the main frame, a supporting pipe is arranged on the upper surface of the second end of the main frame, and an electromagnet is supported at the upper end of the supporting pipe; the electromagnet is provided with a contact indicator lamp, the upper end of the smashing rod is attracted to the electrified electromagnet, and when the lower end of the smashing rod makes contact with the spinal cord of the animal, the contact indicator lamp is turned on. And by arranging the contact indicator lamp, when the lower end of the smashing rod makes contact with the spinal cord of the animal, the contact indicator lamp is turned on, the gauge 0 point is prompted, and height setting is more accurate.

The invention relates to the technical field of in-vitro model construction, in particular to a construction method and application of an elliptical capsule in-vitro injury model. According to the method, the sensory epithelium area of the elliptical capsule is damaged, the elliptical capsule is continuously cultured to construct the in-vitro model, and the constructed in-vitro model of the elliptical capsule can be used for pathogenesis research of flat epithelium, hair cell regeneration research and therapeutic target screening or therapeutic drug screening of vestibular dysfunction.

The application provides doxorubicin and lapatinib in constructing a zebrafish cardiotoxicity damage model and a drug screening method, and belongs to the technical field of biological medicine and animal model construction. The application discloses that doxorubicin and lapatinib can be used in combination to construct a doxorubicin-related zebrafish cardiotoxicity damage model for the first time, and provides a method for establishing a zebrafish cardiotoxicity damage model. The heart index of the application is very comprehensive, and the zebrafish cardiotoxicity damage model constructed by the application can be used to screen drugs of various different action mechanisms which have an improving effect on heart damage.

The present application relates to the technical field of pathological evaluation, and particularly relates to a heat stroke myocardial injury mouse model construction and evaluation method, comprising constructing a heat stroke myocardial injury mouse model by increasing the core body temperature, determining the key time point of the intervention effect of different dose gradients of GDF15 after the heat stroke modeling; obtaining the cTn-I index and the ALT index based on the key time point, determining the injury type ratio based on the normalized values of the cTn-I index and the ALT index to determine the injury type of the heat stroke; adjusting the evaluation strategy in response to the injury type, obtaining the recovery rate of the cTn-I index and the ALT index of the model control group at each time point to determine the myocardial injury degree and the liver injury degree at the corresponding time point; obtaining the average recovery rate of the ALT of each dose group to determine the ALT recovery rate ratio between different dose groups, and adjusting the observation length of the dose group. The present application can distinguish the injury types by constructing the heat stroke myocardial injury model, and cannot adapt to the dynamic changes of different injury types.

The application discloses a device for positioning and quantifying a tibialis anterior muscle injury model of rodents, and belongs to the technical field of medical equipment, which comprises a shearing assembly and a positioning and measuring device, the positioning and measuring device is arranged on the shearing assembly, the positioning and measuring device comprises a measuring assembly for defining the size of a skeletal muscle injury and a positioning assembly for positioning a tibialis anterior muscle part, the measuring assembly is arranged on the positioning assembly, the measuring direction of the measuring assembly is perpendicular to the positioning direction of the positioning assembly, the positioning direction of the positioning assembly is perpendicular to the shearing direction of the shearing assembly, and the lengths of the two ends of the positioning assembly are equal to the length of the output end of the shearing assembly; the device can help researchers to position and quantify the establishment of a tibialis anterior muscle injury model of rodents.

The invention belongs to the technical field of male reproductive toxicity injury model construction in toxicology, and particularly provides a triazole compound induced male testis injury model construction method which comprises the following steps: S1, selecting an experimental rat; s2, a triazole sodium carboxymethyl cellulose aqueous solution with the mass concentration being 4.5 mg / mL to 18 mg / mL is prepared; s3, according to the dosage, the dosage and the administration time, the prepared triazole sodium carboxymethyl cellulose aqueous solution is fed to the experimental rat through oral intragastric administration, the male rat testis injury model is obtained, and the problems that in the prior art, a testis injury model building method is complex in modeling method, long in period, single in end point index, low in success rate and poor in model stability are solved; the modeling method is simple, short in period, high in repeatability, diversified in detection index and low in cost, the success rate of the model can reach 100%, and the model is stable.

The invention belongs to the technical field of animal experiment model construction, and relates to a construction method of a porcine endometrial injury model. The construction method is non-traumatic, can simulate the pathological state of clinical intrauterine adhesion on the premise of not damaging the full-thickness structure of the uterus of a pig, and comprises the following specific steps: intramuscularly injecting 1.5 mg / kg of Shutai to calm an experimental pig, then carrying out anesthesia induction and analgesia, intramuscularly injecting phloroglucinol and probing the cervix uteri after the anesthesia induction succeeds and vital signs are stable, and carrying out intramuscular injection on the experimental pig. The cervix uteri is ensured to be relaxed; a sixth curette enters the uterus body from the cervix gently and slowly, and the endometrium is mechanically scraped until the surface is coarse; and then injecting 95% ethanol and staying for a specific time, and finally removing the residual ethanol. According to the method, laparotomy is not needed, the operation path is simple, the full-layer structure of the uterine muscular layer and the serosal layer can be completely reserved, the modeling effect is highly similar to the clinical intrauterine adhesion condition, the success rate is as high as 95%, the repeatability is good, the modeling effect is stable, and a standardized large animal model capable of being popularized is provided for intrauterine adhesion mechanism research and clinical intervention evaluation.