40 results about "Injury model" patented technology

The invention discloses an application of pentagalloylglucose (PGG) in preparation of a medicine for resisting doxorubicin cardiotoxicity. The method comprises the following steps: inducing doxorubicin (DOX), establishing a myocardial cell H9C2 injury model, and determining the DOX-induced myocardial cell injury minimum concentration; after 20 [mu] M of PGG is added into a treatment group, DOX-induced H9C2 cell apoptosis can be significantly improved. A mouse myocardial injury model is induced and established by DOX, and compared with a doxorubicin group, the left ventricular systolic fraction (FS%) and ejection fraction (EF%) of mice in the doxorubicin and pentagalloylglucose group are remarkably increased, the myocardial fiber structure is improved, the cytoplasm vacuolation is reduced, the serum cTnT and BNP levels are reduced, and the ROS level is reduced. The experiment proves that the pentagalloylglucose can obviously reduce the cardiotoxicity induced by the DOX.

The invention belongs to the technical field of aquaculture, and relates to a thioacetamide-induced procambarus clarkii hepatopancreas injury model construction and evaluation method and application thereof, and the method comprises the following steps: selecting healthy and temporarily cultured procambarus clarkii with the weight of 10.0 + / -1.45 g as an object for establishing the hepatopancreas injury model; fasting the procambarus clarkii for 12 hours, and then giving a thioacetamide solution, wherein the application dosage is 0.4-0.8 mg / g body weight; the thioacetamide is a thioacetamide solution which is prepared from a 0.9 wt% NaCl solution and has the concentration of 40 to 80 mg / mL; the mode of administration of the thioacetamide solution is intramuscular injection. The TAA is applied to construction of the hepatopancreas injury model of the procambarus clarkia for the first time, individual death is not induced in the model construction process, and an ideal experimental model capable of researching pathogenesis of hepatopancreas diseases and screening related therapeutic drugs is obtained. The method has the advantages of being short in period, low in cost, easy to operate, good in repeatability and the like.

The invention provides an establishment method and application of a non-human primate retinal pigment epithelium injury model, and belongs to the field of animal models. The method comprises the following steps: (1) performing anesthesia and mydriasis on a non-human primate; (2) attaching a full retinoscope to the cornea, and adjusting a slit lamp so that an operator can observe the macular fovea centralis, optic disc and upper and lower vascular arches of the non-human primate; and (3) carrying out photocoagulation on the photocoagulation area by using a laser photocoagulator, and ending the photocoagulation until 250-300 primary light spots appear in the photocoagulation area. The invention provides a method for constructing a non-human primate retinal pigment epithelium (RPE) injury model by using laser by taking a non-human primate as a model animal for the first time. The model forming rate of the RPE injury animal model constructed by the invention is stable, the injury caused by the RPE injury animal model is irreversible, and a stable animal disease model is provided for clinical research and development of new drugs related to dry age-related macular degeneration caused by RPE injury.

The invention provides a knee joint personalized rehabilitation scheme generation method and system based on digital twinning, and the method comprises the steps: obtaining the multi-dimensional feature data of a patient to construct a patient rehabilitation data set, constructing a knee joint injury knowledge graph based on the patient rehabilitation data set, constructing a knee joint minimal injury model based on the knee joint injury knowledge graph, and generating a knee joint personalized rehabilitation scheme. Functional weak links and injury degrees of the patient during rehabilitation training are accurately recognized, a data basis is provided for subsequently formulating a personalized scheme for the condition of the patient, emotion extraction is performed on voice feedback data in a patient rehabilitation data set, the patient emotion index is obtained, rehabilitation efficiency diagnosis is performed according to the patient emotion index, and the rehabilitation efficiency of the patient is improved. According to the method, the initial rehabilitation scheme of the knee joint is generated, the initial rehabilitation scheme of the knee joint is subjected to efficiency evaluation and scheme optimization, and the rehabilitation scheme of the knee joint is generated, so that the rehabilitation scheme is dynamically adjusted according to the personalized requirements of the patient while the personalized requirements of the patient are met, and the accuracy and efficiency of the generated rehabilitation scheme are improved.

The invention discloses an adjustable spinal cord impact vibration injury device and an injury method thereof, and belongs to the technical field of spinal cord injury experiment research. Two lifting sliding rods are fixedly installed at the rear end of the base, and lifting sliding blocks capable of vertically sliding are installed on the lifting sliding rods. An angle adjusting mechanism is installed on the lifting sliding block, the movable end of the angle adjusting mechanism is detachably connected with a supporting tool used for fixing the experimental animal, and the angle adjusting mechanism is used for adjusting the angle of the supporting tool so that different parts of the experimental animal can face the base; the base is further provided with an impact triggering mechanism. Through the mode, the experimental animal spinal cord impact oscillation injury model prepared by adopting the technical scheme can well simulate the injury process of a clinical spinal cord injury patient and the pathophysiological change after injury; and the spinal cord impact vibration injury models with different injury modes and different injury degrees are prepared by changing the free falling body height of the experimental animal, the vertical impact rods made of different materials and the impact point location of the experimental animal.

The invention discloses an application of hydroxygenkwanin (HGK) in preparation of a drug for preventing and treating cardiotoxicity induced by antitumor drugs. The method comprises the following steps: inducing doxorubicin (DOX), establishing a myocardial cell H9C2 injury model, and determining the DOX-induced myocardial cell injury minimum concentration; after 20 [mu] M of HGK is added into a treatment group, DOX-induced H9C2 cell apoptosis can be significantly improved. A mouse myocardial injury model is induced and established by DOX, and compared with a doxorubicin group, the left ventricular systolic fraction (FS%) and ejection fraction (EF%) of mice in the doxorubicin and hydroxygenkwanin group are remarkably increased, the myocardial fiber structure is improved, the cytoplasm vacuolation is reduced, the levels of serum cTnT, CK-MB and BNP are reduced, and the ROS level of heart tissue is reduced. Therefore, the hydroxygenkwanin can obviously reduce the cardiotoxicity induced by the DOX.

The invention discloses a manufacturing method of a non-human primate spinal cord half-cut injury model. The spinal cord and the dura mater are exposed in sequence, the dura mater is cut off with the assistance of a dura mater drag hook frame, a positioning guide needle is arranged in the middle axis of the spinal cord through a stereotaxic instrument and a positioning guide needle installed on the stereotaxic instrument, a dura mater protection sheet is arranged, and spinal cord tissue is cut off downwards along a sliding groove of the positioning guide needle through a microscalpel. And a drainage tube is used for indwelling an epidural subfascia for drainage, and a nursing garment is worn, so that manufacturing of a non-human primate spinal cord semi-cutting injury model is realized. According to the method, the modeling consistency and the modeling success rate are ensured.

The invention relates to an establishment method of a high-fat induced intestinal organ injury model and an injury intervention method for the model. The establishment method comprises the following steps: step A, preparation: preparing colon cells, PBMC cells and an organ-like culture chip for later use; step B, colon barrier construction: inoculating colon cells to the surface of a barrier membrane on the lower side of a culture chip by using an organoid culture chip to obtain a colon barrier model; step C, constructing a co-culture model, and inoculating PBMC cells to the basal side of the colon barrier on the basis of the colon barrier to obtain a human colon barrier-PBMC co-culture model; d, constructing a damage model, and performing damage treatment on the co-culture model by using a treating fluid to obtain the damage model; according to the invention, the barrier function organ-like chip is used, a human intestinal cell-PBMC co-culture model is formed by utilizing human intestinal organs and PBMC cells, and OXLDL / palmitic acid can play a role in an injured intestinal organ barrier model and form a high-fat induced intestinal organ injury model through the prepared injury treating fluid.

The invention relates to the technical field of mouse spinal cord modeling, in particular to a mouse spinal cord clamp injury model construction system and method, the system comprises a mechanical arm, a clamp, a data processing and control module and an optical imaging and measuring module, at least one clamping surface of the clamp is provided with a pressure sensor, and the pressure sensor is connected with the mechanical arm. A displacement sensor is arranged on each clamping arm of the clamp; the mechanical arm is connected with the clamp and used for driving the clamp to move, open and close; the pressure sensor, the displacement sensor, the mechanical arm and the optical imaging and measuring module are all in signal connection with the data processing and control module. The defect that in the prior art, due to individual differences of mice, the damage degrees of models under the same pressure are different, and the reliability of experimental results is low is overcome; clamping force can be adjusted according to the diameter of the spinal cord of a mouse, it is ensured that the spinal cords of different sizes are extruded to the same degree, accordingly, injuries with the highly consistent severity degree are generated, and the repeatability and statistical efficacy of experiments can be improved.

The present application relates to the technical field of medical service support, and particularly relates to a personnel vulnerability analysis method for precise medical service support, comprising: target analysis: dividing a human body into parts according to human body functions or professional functions; establishing a human body injury spectrum based on each human body part; constructing an injury model: the plurality of injury models include a projectile injury model, a thermal injury model, a shock wave injury model and an impact vibration injury model; constructing a mapping relationship under various working conditions: calculating physical injury and functional injury under a preset strike working condition based on the injury model; obtaining vulnerability distribution law: performing personnel vulnerability analysis according to the human body injury spectrum and the injury model to obtain the vulnerability distribution law and statistical results under the preset strike working condition. The present application can obtain accurate medical service support data, so that the medical service support data is more stable, scientific, reasonable and accurate.

The invention discloses a carbon tetrachloride damage model construction and nutrition intervention evaluation method based on a three-dimensional liver chip, and belongs to the technical field of microfluidics and biological medicines. According to the method, an in-vitro bionic liver tissue model is constructed by utilizing a three-dimensional liver chip, liver cell injury is induced by perfusing carbon tetrachloride, on the basis, substances such as algae-derived extracellular vesicles are added for repairing intervention, and meanwhile, contrastive analysis is performed with a two-dimensional plate culture system. Results show that the secretion level and the function maintenance time of albumin, urea and the like of liver cells in a liver chip model are superior to those of two-dimensional culture, and after nutrient intervention is carried out, liver injury indexes such as ALT, AST and the like in the chip model are remarkably reduced, and the cell activity and the metabolic function are obviously recovered. The model can be used for evaluating the curative effect of nutrient substances on chemical liver injury, overcomes animal experiment species difference and two-dimensional culture limitation, provides an efficient and sensitive in-vitro research platform for medicine and nutrition function screening, and has wide application prospects and industrialization potential.

The invention provides a method for evaluating the repair activity of a test substance, which comprises the following steps: constructing a 3D epidermis injury model, treating the injured epidermis by using the test substance, detecting the secretion amounts of IL-8 and MCP-1 in the epidermis before and after treatment, and evaluating the repair activity of the test substance based on the secretion amounts of IL-8 and MCP-1, the evaluation result obtained by the method is consistent with the evaluation result of efficacy verification performed by using a human body in trend, the blank of in-vitro evaluation of the injury repair efficacy is filled, a feasible scheme is provided for animal replacement experiments, and a reference basis is provided for further research of skin care product development and efficacy verification.

The invention discloses a construction method and application of an animal skin injury model, and relates to the technical field of biomedicine. According to the construction method, based on the microneedle technology, through the integrated design of parameter quantification control, operation standardization, injury form customization and recovery cycle regulation and control, precision, diversification and standardization of the animal skin injury model are achieved. According to the method, unified operation specifications are formulated by relying on a quantifiable parameter system of a sterile fixed microneedle and combining animal skin characteristics, it is ensured that the damage depth / area deviation of models of the same batch and different batches is controllable, and stable construction of the animal standardized skin damage model is achieved; accurate control of'epidermis-dermis' layered gradient injury is realized through accurate matching of microneedle parameters and skin levels; according to the invention, polymorphic injury customization is also realized: diversified injury types are generated through combination of an operation mode and microneedle parameters; and key design of continuous damage and recovery period regulation and control.

The invention relates to a surgical instrument for aortic valve stenosis caused by guide wire injury and an initiation method, and belongs to the field of construction of animal models of diseases, and the surgical instrument comprises a bottom plate (1) used for placing a mouse; the mouse fixing assembly is arranged on the upper end face of the bottom plate (1) and used for fixing a mouse; the aortic valve injury assembly is arranged on the upper end face of the bottom plate (1) and used for injuring the aortic valve of the mouse. Compared with the prior art, the surgical instrument for treating aortic valve stenosis caused by guide wire injury is provided, and the modeling process of an aortic valve guide wire injury model is scientifically standardized through the surgical instrument.

The application discloses a kind of based on AI mouse cranium brain thoracic and abdominal complex injury dynamic model preparation method and system, belong to the technical field of biomechanics injury.The method comprises: impact is applied to mouse skull, the impact is conducted along organ synergistic conduction chain, to induce the cranium brain thoracic and abdominal complex injury of mouse;In the process of applying impact, multi-modal physiological data are synchronously collected;The multi-modal physiological data collected are input to AI analysis model, the injury mechanism of the cranium brain thoracic and abdominal complex injury is analyzed, and dynamic model is established based on analysis result.The technical scheme of the present application realizes the high simulation modeling of multiple organ cascade injury by constructing organ synergistic conduction chain from cranium to thoracic cavity;Combined with multi-modal physiological data synchronous acquisition and AI analysis model, the injury timing characteristics can be accurately extracted, the causal path of local injury triggering system response is established, and the accuracy and practicality of complex injury mechanism prediction are improved.

The invention relates to the technical field of image analysis of injury models, in particular to an in-vivo and in-vitro modeling method for myocardial infarction ischemic injury, which comprises the following steps of: determining an injury dilation prediction sequence of each myocardial sub-region by pre-constructing a blood supply association relationship between each myocardial sub-region and each blood supply artery; the method comprises the following steps: determining whether a myocardial infarction and ischemic injury sample model is qualified or not by acquiring the confidence coefficient of each myocardial infarction region contour section, pre-regulating and controlling the injury of the ischemic injury, redefining the myocardial infarction region contour section with the suspected confidence coefficient, and judging whether the myocardial infarction and ischemic injury sample model is qualified or not according to the difference quantity of injury characterization quantities in a continuous injury identification period. According to the method, a GDF-15 research test is carried out on a qualified myocardial infarction ischemic injury sample model, so that the injury expansion of an infarction area is predicted in the continuous batch construction process of the myocardial infarction ischemic injury model, the model construction process is regulated and controlled in a targeted manner, and the uniformity and effectiveness of continuous construction of the model are improved.

The invention discloses a coevolution algorithm-based automobile side collision optimization design method, which comprises the following steps of: firstly, constructing a passenger injury model, an automobile body structure deformation model and an automobile body weight model based on an injury mechanism in a collision accident, and providing a basis for subsequent optimization; through a multi-objective optimization model, objectives of passenger safety, a vehicle body structure, lightweight design and the like are considered, and strict constraint conditions are set. And a coevolution algorithm is utilized, and coevolution of the main population and the auxiliary population is combined. By combining the constraint processing technology, it is ensured that the design scheme meets the actual collision safety requirement. And finally, through optimization, an optimal automobile structure design scheme which not only meets the safety requirement but also has good performance can be output.

The invention discloses a construction method and application of a zebra fish tail fin ischemia injury model with both inflammatory response and regeneration capacities, rose bengal is injected into the abdominal cavity of a zebra fish, then the zebra fish tail fin ischemia injury model is constructed by performing local fluorescence irradiation on the tail fin of the zebra fish and performing photochemical induced injury, the operation is simple and convenient, and the construction method is suitable for large-scale popularization and application of the zebra fish tail fin ischemia injury model. And the death rate of the zebrafish in one day after modeling of the zebrafish is very low (only 5.3%), and the method has good repeatability and operability. The photochemically induced caudal fin injury model has pathological characteristics closer to clinical tissue injury, can simulate inflammation and regeneration processes at the same time, and has dual application potentials of drug screening and mechanism research.

The present application relates to the technical fields of lower limb biomechanics analysis, and especially relates to a model system suitable for lower limb biomechanics analysis and a method thereof.The technical scheme comprises the following modules: an input module for inputting bone parameters, soft tissue parameters and injury models; a calculation module for performing calculation analysis; and an output module for outputting analysis results; wherein the input module comprises a bone parameter module for establishing a bone model based on collected lower limb CT images, and performing parameterized modeling on a lower limb three-dimensional model.The present application provides a fast and efficient analysis system for lower limb biomechanics analysis, and can quickly, accurately and directly establish a lower limb model according to CT and MRI data of a subject and required parameters, perform finite element analysis, and obtain the influence of different degrees of varus, valgus, internal rotation, external rotation, meniscus tear injury, ligament injury, cartilage injury and the like on knee joint biomechanics, which has great significance for the development of lower limb biomechanics.

The application belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition for protecting gastric mucosa and application. The raw material of the traditional Chinese medicine composition is Corydalis and Fructus Meliae, and the preparation method comprises the following steps: firstly, Corydalis is extracted with water, and filtered to obtain Corydalis extract and Corydalis residue; then, Fructus Meliae and the Corydalis residue are mixed with water, and enzyme is added for enzymolysis to obtain an enzymolysis solution; finally, the Corydalis extract and the enzymolysis solution are mixed, sterilized, fermented with probiotic bacteria liquid, and filtered to obtain the traditional Chinese medicine composition. The composition prepared by the preparation method has a significantly better protection effect on gastric mucosa than other methods due to the reasonable combination of Fructus Meliae and Corydalis, can significantly reduce the gastric mucosa lesion degree of experimental gastric mucosa injury model animals, and improve the repair ability of gastric mucosa.

The invention relates to a PFOS corticobrain organ injury model and a construction method and application thereof. The method comprises the following steps: (1) culturing corticobrain organs; (2) performing PFOS exposure on the cultured cortical brain organ to obtain a PFOS cortical brain organ injury model; (3) performing RNA-seq sequencing on the PFOS corticobrain organ injury model, and identifying whether the PFOS corticobrain organ injury model is successfully constructed or not through a molecular mechanism; the molecular mechanism comprises at least one of a P53signaling pathway and an axon guiding pathway, and the molecular mechanism comprises at least one of a P53signaling pathway and an axon guiding pathway; further, if the molecular mechanism appears, the PFOS cortical brain organ injury model is successfully constructed. The PFOS cortical brain organ injury model constructed by the method can promote deep research on PFOS injury brain pathological mechanisms, and a new perspective is provided for understanding the development process of diseases.

The invention discloses an artificial intelligence dynamic evaluation mouse multiple injury model construction method and system, and belongs to the technical field of injury models. The method comprises the steps that controlled impact is applied to the brain of a mouse to cause brain injury, and controlled trauma is introduced into the thoracic cavity and the abdominal cavity to simulate thoracic cavity and abdominal cavity injury; physiological parameters of the injured mouse are monitored in real time through a sensing device, and physiological data reflecting craniocerebral, thoracic and abdominal injury influences are collected; inputting the physiological data into an artificial intelligence algorithm model, evaluating the injury severity of the injured mouse in real time and generating a corresponding evaluation result; the evaluation result is used for grading the severity of the injury, and a corresponding mouse craniocerebral combined thoracic and abdominal multiple injury model is established according to the grading result. According to the technical scheme, accurate simulation and grading evaluation of multi-part injuries of the mouse are achieved, and multi-injury mechanism exploration and intervention strategy effect verification are effectively supported through multi-modal physiological parameter real-time monitoring and artificial intelligence dynamic analysis.

The present invention discloses an adjustable spinal cord impact vibration injury device and an injury method thereof, which belongs to the technical field of experimental research on spinal cord injury, and comprises a base; two lifting slide bars are fixedly installed on the rear end of the base, and a lifting slider that can slide vertically is installed on the lifting slide bar; an angle adjustment mechanism is installed on the lifting slider, and the movable end of the angle adjustment mechanism is detachably connected to a brace for fixing the experimental animal, and the angle adjustment mechanism is used to adjust the angle of the brace so that different parts of the experimental animal face the base; an impact trigger mechanism is also provided on the base. Through the above method, the experimental animal spinal cord impact concussion injury model prepared by adopting this technical solution can well simulate the injury process and post-injury pathophysiological changes of clinical spinal cord injury patients, and by changing the free fall height of the experimental animal, the upright impact rods of different materials and the impact point of the experimental animal, spinal cord impact concussion injury models with different injury modes and different injury degrees can be prepared.

The invention provides a skin injury model making device which comprises an environment gas preparation box, a pipeline and a model stimulation box, and the environment gas preparation box discharges prepared environment simulation airflow to the surface of a skin model in the model stimulation box through the pipeline so as to stimulate the skin model to form a skin injury model. According to the requirements of a skin model, natural climate is simulated by adjusting parameters such as gas temperature, humidity or gas flow to perform injury modeling on the skin, and diversified skin injury models are prepared.

The invention discloses a verification method of a rat neuropathic pain model, and relates to the field of clinical medicine. Comprising the following steps: animal preparation: selecting healthy adult rats, and putting the rats in a laboratory environment for adaptive feeding; model construction: constructing a sciatic nerve branch selective injury model; the pain behavioral test comprises a foot sole hot stabbing pain experiment, a mechanical pain sense test and a cold pain sense test; data analysis: performing data analysis according to a behavioral test result; and model verification: verifying whether the construction of the model is successful or not by observing the behavioral performance of the rat, comparing the symptoms of the model rat with the symptoms of a human neuropathic pain patient, and evaluating the clinical similarity of the model. According to the method, the neuropathic pain model is successfully induced and verified through SNI model surgical operation and hot plate testing, and the method has high reliability and repeatability and provides powerful support for research of neuropathic pain.

The present invention discloses a THC-induced in vitro blood-brain barrier injury model, a construction method and its application, including: preparing a THC solution; evaluating the toxic effect of THC on hCMEC / D3 and determining the dosing dose and dosing time; constructing a co-culture BBB model; evaluating comprehensive indicators of the blood-brain barrier after the action of THC. The present invention constructs a co-culture BBB model of hCMEC / D3 cells and U-87MG cells, clarifies the detailed construction process and evaluation indicators, improves the problem that THC is difficult to be directly administered by water-soluble dissolution, comprehensively evaluates the pathological change process of THC-induced BBB injury, and provides a reference for the precise use of THC. The present invention provides a more real and accurate THC-induced BBB injury model, and this model provides a more effective tool for studying the influence of THC on the central nervous system and its potential neurotoxicity.

The invention belongs to the field of medicines and application thereof, and particularly relates to establishment of a working memory impairment model and evaluation of the treatment effect of butylphthalide on working memory impairment caused by prefrontal cortex ischemia. The model is an animal model for working memory impairment caused by prefrontal cortex (mPFC) ischemia caused by endothelin (ET-1), and an intervention measure is butylphthalide medicine. By detecting related indexes of T maze, Y maze, white matter integrity, oligodendroglia cells (OLs) and ferroptosis of a mouse, it is evaluated that a model of working memory impairment caused by mPFC ischemia caused by ET-1 is established, and after the mouse is treated with butylphthalide, the working memory impairment of the mouse is improved, the white matter integrity is enhanced, and the OLs ferroptosis condition is reduced.

The embodiment of the invention provides an animal spinal cord full transection injury model and a manufacturing method thereof. The method comprises the following steps: providing an experimental spinal cord animal and a self-made trocar needle assembly; determining the position of a T9-T10 vertebral plate of the spinal animal, cutting the skin at the position, and separating muscles and ligaments to expose the gap between the T9-T10 vertebral plate; two opposite sides of the back side of the spinal cord are selected as needle inserting points, the cannula needle head assembly is inserted into the T9-T10 intervertebral plate gap from the needle inserting points, an inner needle core is withdrawn after the dural sac is punctured, and an outer cannula is inserted into the puncture point; repeatedly drawing and inserting the outer sleeve to mash the spinal cord tissue in the dural sac, and sucking out the mashed spinal cord tissue through an external aspirator. According to the embodiment, the vertebral plate does not need to be removed, manufacturing of the injury model is completed on the basis that the integrity of the dura mater is reserved as much as possible, the integrity of blood vessels is reserved to the maximum extent, and the influence of vascular injury on nerve regeneration is eliminated to the maximum extent.

The invention specifically discloses a platelet injury assessment method and device for a hemodialysis catheter, a medium and a program product, and the method comprises the steps: obtaining the actual structural characteristics and clinical standard size of the hemodialysis catheter, and carrying out the geometric modeling of the hemodialysis catheter; performing high-precision grid division and hemodynamic analog simulation on the geometric model of the hemodialysis catheter; setting boundary conditions of each fluid domain, and establishing a shear stress and vorticity coupled damage evaluation mechanism by introducing vorticity as coupling characterization of local shear stress and eddy current effect; calculating the vortex intensity and shear stress of the blood of the blood flow velocity field through the damage assessment mechanism, and constructing a blood damage model coupled with a time accumulation effect; particle statistical data are obtained through particle tracking in the hemodialysis catheter, and platelet injury calculation is carried out through a blood injury model. The accuracy of platelet injury estimation of the hemodialysis catheter can be improved.

The invention provides a drug screening method and device based on a blood-brain barrier injury model and a medium, and the method comprises the steps: collecting experimental metadata and biological sample measurement data of the blood-brain barrier injury model, the experimental metadata comprises an identifier of a candidate drug, an animal identifier, a body weight, an Evans blue injection volume, circulation time, perfusion time and a sampling position, and the biological sample measurement data comprises a brain tissue weight and a fluorescence intensity measurement value; converting the biological sample measurement data into a quantitative index of blood brain barrier leakage, wherein the quantitative index is the Evans blue leakage amount per gram of brain tissue; and generating a prediction effect of the candidate drugs according to experimental metadata and the quantitative indexes. The blood-brain barrier leakage can be rapidly and accurately evaluated through Evans blue staining and fluorescent quantitative technologies.