76 results about "Mouse skin" patented technology

The invention discloses pediococcus pentosaceus CCFM1402 capable of effectively repairing a skin barrier and a self-fermentation metabolite of the pediococcus pentosaceus CCFM1402, and belongs to the technical field of microorganisms and medicines. The pediococcus pentosaceus CCFM1402 provided by the invention has the effect of improving the skin barrier function in both external use and oral administration; the cell activity of damaged HaCaT cells and the expression of the gene level of skin barrier function protein and tight junction protein can be improved by virtue of a thallus lysate and a fermented supernatant (metagen) of the external pediococcus pentosaceus CCFM1402; and the DNFB-induced mouse skin barrier injury can be relieved in the aspects of appearance, skin barrier protein expression and the like by orally taking viable bacteria and postbiotics of the pediococcus pentosaceus CCFM1402. Therefore, the pediococcus pentosaceus CCFM1402 has a huge application prospect in preparation of external or oral medicines for repairing the skin barrier and preparation of food, health care products or cosmetics.

The invention relates to application of a tea exosome in preparation of a medicine for improving skin pigmentation, the tea exosome is creatively extracted and purified, and the tea exosome can effectively inhibit proliferation of melanoma cells and inhibit expression of melanin generation related protein by scavenging free radicals and inhibiting tyrosinase activity, so that the effect of improving skin pigmentation is achieved. The intracellular melanin removal is promoted. In a mouse in-vivo experiment, after the tea exosome is applied, it is observed that the skin epidermis thickness of a mouse is reduced, the dermis thickness is increased, collagen arrangement is tighter and more ordered, the content is increased, and meanwhile generation of inflammatory factors is reduced. The tea exosome can significantly increase the content of a melanin autophagy key factor LC3B and reduce the content of P62 to promote melanin autophagy, and has good percutaneous infiltration capacity, thereby playing a role in improving skin pigmentation.

The invention belongs to the technical field of microorganisms, and particularly relates to an extracellular vesicle of phytobacterium plantarum and application of the extracellular vesicle. Specifically, the plant lactobacillus extracellular vesicles can promote HaCaT cells to generate ceramide, up-regulate expression of key genes in a skin ceramide synthesis route and recover a skin barrier; the expression of proinflammatory factors in a cell inflammation model can be reduced, and a relatively good anti-inflammatory effect is achieved. A mouse skin injury model verifies that the EVs has good skin infiltration capacity and can remarkably reduce the skin injury score, relieve skin itch, improve the skin water content and reduce expression of proinflammatory factors in skin tissue and expression of pruritus-related cell factors.

The invention relates to application of VBIT-4 or pharmaceutically acceptable salt thereof in preparation of drugs for preventing or treating vitiligo and complications thereof. A large number of modern pharmacological scientific researches and in-vivo pharmacodynamic tests prove that VBIT-4 has a treatment effect on a leucoderma mouse model and inhibits synthesis of inflammatory factors and chemotactic factors in a leucoderma mouse skin lesion area. The mechanism of the invention is that VBIT-4 inhibits the expression of inflammatory factors and chemotactic factors in skin tissues so as to treat leucoderma, compared with the existing treatment means, VBIT-4 can promote the compound color of pigments in a skin lesion region on the back of a leucoderma mouse, has no obvious toxicity, and reduces the expression level of inflammatory factors (IL-1beta, IL-6 and IFN-alpha) and chemotactic factors (CXCL9 and CXCL10). In conclusion, the VBIT-4 is clear in action mechanism, remarkable in effect and free of obvious side effects, and has remarkable advantages and progress compared with existing drugs.

The invention discloses a modeling device for a mouse skin light aging experiment, and relates to the technical field of skin light aging experiments, the modeling device comprises a box body, a box cover is hinged to the upper end of the box body, a bottom plate is fixedly arranged in the box body, a plurality of fixed partition plates are arranged at the upper end of the bottom plate, and adjusting partition plates are arranged on one sides of the fixed partition plates; fixed sliding plates are arranged on one sides of the fixed partition plates in a sliding mode, telescopic sliding plates are arranged at one ends of the fixed sliding plates in a sleeving and sliding mode, a linkage mechanism is arranged on one side of the box body, and irradiation components are arranged in the box cover and above the experiment channel. The linkage mechanism drives the adjusting partition plate, the fixed sliding plate and the telescopic sliding plate to move, so that when an irradiation experiment is carried out, the space of an experiment channel can be reduced, the activity range of a mouse is limited, and the distance between the two ultraviolet lamp sources and the irradiation angle of the ultraviolet lamp sources can be adjusted through the adjusting mechanism; therefore, irradiation in different states can be simulated conveniently.

The invention relates to the technical field of animal umbilical cord extraction, in particular to a preparation method and application of an animal umbilical cord extract. The trypsin mutant is adopted to prepare the umbilical cord extract, the enzymolysis efficiency is improved, the skin repairing effect of the obtained umbilical cord extract is remarkably superior to that of a common enzymolysis product, and in repairing of a mouse burn model, the recovery speed of mouse skin is 2 days earlier than that of a common umbilical cord extract. The umbilical cord extract prepared by the method provided by the invention is expected to be widely applied to wound or skin repair.

The invention relates to the technical field of biological medicines, in particular to a soluble skin-adhering microneedle for delivering an inactivated enterovirus vaccine as well as a preparation method and application of the soluble skin-adhering microneedle. Sodium hyaluronate is adopted as a film forming material to wrap an enterovirus 71 type (EV71)-coxsackie virus A16 (CA16) type bivalent inactivated vaccine antigen, and a needle body layer of the soluble microneedle is formed; the substrate layer is prepared from a pullulan material, so that the microneedle patch with a stable overall structure is formed. The technology provided by the invention is completed in a clean environment, and the vaccine patch which is qualified through sterility detection is obtained. The soluble microneedle disclosed by the invention has good antigen stability under the condition of 2-8 DEG C. In animal experiments, the microneedle is attached to the skin surface of a mouse for skin-adhering immunization, a strong immune response reaction can be induced, good immunogenicity is shown, and a novel, safe and effective vaccine delivery way is provided for prevention and control of the infantile hand-foot-and-mouth disease.

The invention discloses an application of an exopolysaccharide TDG3 in preparation of a product for promoting synthesis of skin collagen. The structural formula of the exopolysaccharide TDG3 is # imgabs0, and the exopolysaccharide TDG3 can remarkably promote mouse skin collagen synthesis and wound healing, has the advantages of high stability, easiness in obtaining, high safety, low production cost and the like, is suitable for being prepared into a composition or a product for promoting skin collagen synthesis and protecting and repairing a skin barrier, and has a wide application prospect.

The invention discloses lactobacillus reuteri CCFM1403 with a skin repairing effect and a metagen of the lactobacillus reuteri CCFM1403, and belongs to the technical field of microorganisms and medicines. The lactobacillus reuteri CCFM1403 provided by the invention has the effect of improving the barrier function of the skin in two aspects of external use and oral administration, and is specifically shown in that when the thallus lysate of the lactobacillus reuteri CCFM1403 is externally used, the cell viability and the related barrier function of keratinocytes after injury and the expression of a structural protein gene level can be improved; and the DNFB-induced mouse skin barrier injury can be relieved in the aspects of TEWL in apparent aspect, water content of cuticle, skin barrier protein expression and the like by orally taking viable bacteria and postbiotics of the lactobacillus reuteri CCFM1403. Therefore, the lactobacillus reuteri has a huge application prospect in preparation of external or oral medicines for repairing the skin barrier and preparation of food, health care products or cosmetics.

The invention provides lignan, and belongs to the technical field of medicines. According to the invention, a compound shown as a formula I and a compound shown as a formula II are extracted, separated and identified from schisandra chinensis oil for the first time, and the compound shown as the formula I and the compound shown as the formula II are superior to a positive drug group arbutin in the aspect of inhibiting generation of melanin of mouse skin melanoma cells (B16F10); an inflammatory factor release inhibition experiment research shows that the compound shown in the formula I and the compound shown in the formula II have relatively good activity in the aspect of inhibiting LPS-induced mouse mononuclear macrophage RAW264.7 from releasing IL-6 and TNF-alpha; in a skin irritation experiment, the schisandra chinensis non-saponifiable matter containing the compound shown in the formula I and the compound shown in the formula II is non-irritant to the skin of a white rabbit, and it is proved that the compound shown in the formula I and the compound shown in the formula II can be applied to preparation of whitening and anti-inflammatory products. Formula I; formula II.

The invention discloses a preparation method of a mouse skin organoid, which comprises the following steps: separating dermis and epidermis of an E18.5 mouse to obtain dermis cells, mixing the dermis cells with back skin cells of an E14.5 embryo mouse, mixing by using Matrigel to form cell spheres, and gradually forming the skin organoid in in-vitro culture; the organoid is mainly applied to batch production of organoid containing hair follicles. The invention relates to the technical field of stem cells and regenerative medicine. According to the preparation method of the mouse skin organoid, a cell source is improved in the experiment process, the mouse skin organoid containing hair follicles can still be formed after cells in the early development stage are added, and related developmental biology research is carried out. And the source of another dermal cell is not limited to late pregnancy, and also can be a newborn mouse, so that the source of seed cells is expanded, and the cost is reduced.

The invention relates to the field of tissue dissociation and cell separation, and discloses a method for rapidly digesting mouse skin tissue into single cells. According to the method, the skin tissue on the back of an adult mouse is taken as an example, different enzyme digestion modes and mechanical treatment modes are combined and used, and the optimal dissociation condition is screened out, so that the skin tissue can be dissociated into the single-cell suspension within a short time. According to the technical scheme, the efficient dissociation of the skin tissue is realized through the optimal combination of the enzyme type, the concentration and the mechanical treatment mode, and the required treatment time is greatly shortened; the cell purity and the cell viability are effectively improved, and an ideal cell sample is provided for subsequent single cell omics research; and reliable technical support is provided for subsequent flow cytometry analysis, cell sorting, high-throughput sequencing and other researches.

The invention discloses a lactobacillus casei exosome capable of improving skin aging and a preparation method of the lactobacillus casei exosome. The lactobacillus casei is lactobacillus casei RH99, and the preservation number of the lactobacillus casei exosome is CGMCC (China General Microbiological Culture Collection Center) No.32108. The lactobacillus casei RH99 exosome prepared by the preparation method disclosed by the invention has relatively strong acid resistance, and can be orally taken to reach the intestinal tract part; according to the prepared lactobacillus casei RH99 exosome, the ROS level in H2O2 induced HaCaT cells can be reduced, the expression of collagen and elastin genes COL1A1-ELN can be activated, the expression of inflammation pathway related genes TNF-alpha and IL-6 can be reduced, and inflammation damage can be reduced; the composition can inhibit the increase of MDA content in light aging skin of mice, improve the activity of antioxidant enzymes such as SOD, GSH-PX and the like, improve the content of HYP, increase the moisture content of skin and play a role in delaying skin aging; the content of TNF-alpha, IL-6, IL-18 and IL-1beta inflammatory factors in mouse serum can be reduced; the invention has important application significance and research value in preparation of products for preventing or improving skin inflammation, oxidative damage and skin aging.

The invention discloses a high-bionic in-vivo immune microenvironment in-vitro skin model and application, and belongs to the technical field of biological medicine. The preparation method of the in-vitro skin model of the high-bionic in-vivo immune microenvironment comprises the following steps: firstly, adding a culture medium into the lower layer of a six-pore plate, then adding a mouse bone marrow cell suspension, then putting the six-pore plate into a Transwell chamber, flatly laying separated mouse skin on an absorptive gelatin sponge, putting the absorptive gelatin sponge into the Transwell chamber, adding the culture medium into the Transwell chamber, and then putting the mouse skin into the transwell chamber to obtain the in-vitro skin model of the high-bionic in-vivo immune microenvironment. Placing the six-hole plate in a cell incubator for culturing to obtain the in-vitro skin model. According to the in-vitro skin-bone marrow cell co-culture system, an immune environment is provided for the in-vitro skin by introducing bone marrow cells, so that the culture environment of the in-vitro skin is more complete, the difficulties that cell components are simple and the immune environment is deficient at present can be solved, and the in-vitro skin-bone marrow cell co-culture system is more in line with the 3R principle and is high in bionic property and low in cost.

The utility model relates to the technical field of biological experiments, and discloses a simple rat and mouse skin drag hook which comprises a drag hook body, and the drag hook body serves as an integral part of the whole drag hook, serves as a main basic component of surgical operation and is further used for assembling and bearing a lower structural component which the drag hook body belongs to. The skin hooking area is used as a skin hooking function part of the whole retractor body during an operation; the two combination areas are used as parts with a supporting function during storage and placement; the hanging area is used as a fixing part during surgical operation; and a suspension assembly. According to the utility model, in rat or mouse experimental research, a scalpel is used for cutting open the skin surface, an operator pinches the hanging area to penetrate the skin hooking area into the skin to stretch so as to expand space, a needle tube penetrates through the inner side of the hanging area, and the needle tube is inserted into a table top, so that the drag hook body can be fixed, or the drag hook body is fixed by a pull rope; assistants are not needed, the operation space is enlarged, and the operation is more convenient.

The invention discloses an operation table for dissecting rats and mice, and relates to the technical field of mouse dissecting tables, the operation table comprises an experiment assembly and a laparotomy assembly, and the experiment assembly comprises a working plate; the laparotomy assembly comprises a rotating frame, a deformation plate and a clamping plate; the rotating frame is rotationally connected to the working plate through a friction hinge and is used for placing a mouse; during laparotomy and mouse observation, the rotating frame can drive the whole mouse to rotate, and the wound orientation is adjusted; a clamping plate is arranged at one end, far away from the rotating frame, of the deformation plate; the deformation plate is made of a bendable iron material, and the clamping plate is used for clamping and unfolding the epidermis of a mouse; according to the device, an operator can adjust the angle and the distance between the two clamping plates at will through the deformation plate capable of being manually bent, so that the device can be perfectly attached to mice of various body types from small size to large size, the problem that the belly is not fully lifted due to too loose clamping or the chest is pressed due to too tight clamping is solved, and a stable foundation is provided for anatomy operation.

The utility model relates to the technical field of cell collection, and discloses a mouse skin cell collection brush which comprises a shell, a sliding block is slidably connected to the interior of the shell, a rotating block is rotatably connected to the rear end of the sliding block, a connecting column is fixedly connected to the top end of the rotating block, a fixed block is slidably connected to the interior of the sliding block, and the connecting column is fixedly connected to the top end of the rotating block. The outer portion of the connecting column is slidably connected to the outer portion of the shell, a brush is fixedly connected to the front end of the fixing block, a limiting assembly used for limiting is arranged outside the connecting column, a dismounting and mounting assembly used for dismounting and mounting is arranged inside the rotating block, and the limiting assembly comprises a sliding block. The interior of the sliding block is connected to the exterior of the connecting column in a sliding mode. According to the utility model, the length of the brush can be adjusted, so that the applicability and the collection efficiency of the collection work aiming at different parts are greatly improved, and the whole collection process is more scientific, convenient and efficient.

The invention discloses an integrated experimental device for mouse skin photoaging modeling, and relates to the technical field of skin photoaging modeling. The mouse fixing device comprises a fixing main plate and a plurality of semicircular fixing cylinders. The fixed main board is erected in the experiment box body and is provided with a plurality of groups of fixed cylinder slots; the semicircular fixed cylinder is arranged in the fixed cylinder slot in a pluggable manner; a cylinder body of the semicircular fixing cylinder is provided with a fixing hole through which an elastic binding piece penetrates, and the head end of the semicircular fixing cylinder is provided with an integrally-formed arc-shaped baffle used for shielding the eyes of the mouse during ultraviolet irradiation. The stress intervention system is integrated in the experiment box body and comprises a blue light phototaxis sedation module and an olfaction pacification spraying module; the blue light phototaxis sedation module comprises at least one group of blue light sources arranged in the box body and is used for inducing mice to settle; the olfaction pacifying spraying module comprises an atomizer and a spraying outlet and is used for releasing pacifying aerial fog into the box body, multiple functions of animal fixing, ultraviolet irradiation, eye protection and stress control are synchronously completed in a single device, and the standardization degree and data reliability of light aging modeling are remarkably improved.

The utility model relates to the technical field of mouse experiments, and discloses a thermocouple for measuring the temperature of a mouse, which comprises a thermocouple body connecting end, and one side of the thermocouple body connecting end is fixedly connected with an auxiliary end. Electrical connection between the coupling end of the thermocouple body and the thermocouple wires can be realized by utilizing the thermocouple pins, and the two thermocouple wires form different shapes, such as a fixed knot, a spiral shape and an annular shape, so that a suture line can smoothly penetrate through a gap, the thermocouple wires are fixed with the mouse skin, the contact area with the mouse skin is increased, and the service life of the thermocouple is prolonged. The second fixing block can reinforce the connection between the thermocouple pin and the connecting end, thereby improving the stability of the connection between the thermocouple body connecting end and the protective net, reducing the shaking of the thermocouple body connecting end at the top of the protective net when the thermocouple body connecting end is used, and improving the temperature measurement accuracy of the mouse. The connection end of the thermocouple body is prevented from falling off.

The invention discloses a construction method of a sensitive skin animal model, and belongs to the technical field of animal model construction. According to the method disclosed by the invention, the sensitive skin animal model is constructed by smearing the lauryl sodium sulfate on the back skin of the mouse after hair removal in combination with veratrine or capsaicin, so that physiological indexes of human sensitive skin can be simulated; key pathological processes of human sensitive skin can be simulated by detecting key pathological indexes such as skin barrier damage, nerve and blood vessel high reactivity, immune inflammation and the like, natural pathophysiological processes of human sensitive skin can be simulated, and pathogenesis of sensitive skin is basically covered; barrier destruction of mouse skin, high reactivity of nerve and blood vessels and inflammatory response are induced, and clinical practice is met. In addition, the method is good in repeatability, high in safety and easy and convenient to operate, sensitive skin medicine screening and personalized treatment scheme development can be promoted, the research and development period of sensitive skin related treatment schemes is shortened, and the application prospect is good.

The invention discloses a lactobacillus rhamnosus LR-C111 contributing to skin aging resistance and application of the lactobacillus rhamnosus LR-C111. The lactobacillus rhamnosus LR-C111 is classified and named as lactobacillus rhamnosus, the preservation number is CCTCC NO: M 20251647, the preservation unit is China Center for Type Culture Collection, and the preservation time is July 21, 2025. The lactobacillus rhamnosus LR-C111 is named as lactobacillus rhamnosus LR-C111. The lactobacillus rhamnosus LR-C111 can enhance the antioxidant enzyme activity of mouse skin and reduce the generation of oxygen free radicals by inhibiting the expression of MMP-1 and MMP-9 pathways, so as to inhibit the content decomposition of endogenous components in the skin and reduce the loss of skin collagen and the degradation of ECM (extracellular matrix), thereby delaying the skin aging. The composition can be used for preventing, treating and / or improving skin elasticity reduction, skin wrinkles and other symptoms caused by skin photoaging caused by ultraviolet rays.

The invention discloses a stilbene derivative, a preparation method thereof and application of the stilbene derivative in preparation of drugs for treating psoriasis or ulcerative colitis, and belongs to the technical field of biological medicines. The compounds II-2 and II-23 provided by the invention are used for targeted excitation of AHR related pathways, promotion of AHR nucleation, reduction of secretion of related cytokines at a cellular level, and substantial improvement of mouse skin symptoms and PASI scores of an imiquimod-induced mouse psoriasis model, compared with a lead compound benvimod, the compounds II-2 and II-23 have better psoriasis resistance activity and lower toxicity, and can be used for preparation of drugs for treating psoriasis. Meanwhile, the compound II-23 is found to be capable of effectively treating mouse ulcerative colitis, reducing inflammatory response and improving colitis symptoms. The compound can be used for preparing medicines for treating psoriasis and ulcerative colitis.

The utility model discloses a wound surface preparation device for a pressure sore mouse model, which particularly belongs to the technical field of magnetic sheet method pressure sore mouse model construction and comprises a wound surface dehairing mechanism and a wound area measuring frame. The wound surface dehairing mechanism comprises a dehairing blade, a fixed disc, a dehairing net, a rotating rod and a driving motor; the output end of the driving motor is fixed with the rotating rod; the dehairing blade is fixedly arranged at the side end of the fixed disc; the dehairing net is provided with holes for mouse hair to penetrate through, and the fixing disc is located in the dehairing net. And the motion range of the dehairing net is within the wound area measuring frame. The dehairing area of 1 cm < 2 > can be rapidly prepared through the pressure sore mouse model wound surface preparation device. Meanwhile, through isolation of the dehairing net, direct contact between the blade and the mouse skin is avoided, and damage to the mouse skin caused by the blade is prevented.

The present invention discloses a method for constructing an animal inflammatory model of psoriasis with different severities of skin lesions, comprising the following steps: raising 30 Balb / C mice according to a standard feeding method; weighing and calculating the body surface area of ​​each mouse at baseline D0, and removing the hair of the mice; randomly dividing the 30 Balb / C mice into three groups according to the lesion area, namely a mild group, a moderate group and a severe group; using 5 mg / cm 2 Imiquimod was evenly applied to the lesion area of ​​each mouse; the mice were killed after 7 days of intervention, and the skin tissue and spleen of the mice were taken for subsequent examination. The changes in the skin lesions of the mice were recorded by taking photos with a camera. After 7 days of treatment with imiquimod cream, typical psoriasis-like lesions appeared. The constructed psoriasis animal model has very similar clinical symptoms to psoriasis patients, can show psoriasis-related histological changes and immune system changes, and can be used to screen psoriasis treatment drugs.

The present invention relates to the technical field of animal umbilical cord extraction, and particularly relates to a preparation method and application of an animal umbilical cord extract. By using a trypsin mutant to prepare the umbilical cord extract, the present invention not only improves the enzymatic hydrolysis efficiency, but also the obtained umbilical cord extract is significantly superior to ordinary enzymolysis products in terms of skin repair effect. In the repair of a mouse burn model, the recovery rate of the mouse skin is 2 days earlier than that of the ordinary umbilical cord extract. The umbilical cord extract prepared by the method provided by the present invention is expected to be widely used in wound or skin repair.

The invention relates to application of bornyl acetate and an external gel preparation thereof in preparation of a medicine for relieving or treating chronic pruritus. Compared with the prior art, the bornyl acetate separated from volatile components of the folium artemisiae argyi supercritical extract has remarkable effects of inhibiting mast cell degranulation and inhibiting expression of pruritus-related factors at a cellular level and an animal level, and can be used for remarkably relieving mouse skin injury caused by DNCB; the polypeptide has the advantages of good safety, high selection index and the like, can be used for developing drugs for relieving and / or treating chronic itching and expanding treatment schemes of chronic itching, and has wide application prospects and important clinical significance.

The present invention discloses a composition having the efficacy of preventing hair loss and promoting hair growth, and its application. By combining ginsenoside CK and biochanin A, the effects of preventing hair loss and promoting hair growth and their synergistic effects are achieved. The specific technical solution of the present invention is as follows: a composition having the efficacy of preventing hair loss and promoting hair growth, comprising ginsenoside CK and biochanin A; the weight ratio of ginsenoside CK to biochanin A is 1:0.5-3. The composition can be used in the preparation of anti-hair loss products. The present invention combines ginsenoside CK and biochanin A. The combination of the two promotes hair growth primarily by enhancing the antioxidant capacity of mouse skin tissue. Within a certain ratio range, the antioxidant capacity of mouse skin tissue can be synergistically improved. Within a certain ratio range, ginsenoside CK and biochanin A can synergistically promote hair growth.

The application discloses synthesis of a novel Schiff base gold (III) compound and antibacterial application thereof, and belongs to the technical field of medicine preparation; gold sodium aurothiomalate has significant antibacterial activity, but the Au-S bond thereof is easily broken by intracellular reducing thiol, and the gold sodium aurothiomalate is metabolized before reaching a target point to produce toxic side effects; based on the gold sodium aurothiomalate, developing a gold compound with stronger stability is one of strategies for overcoming drug-resistant bacteria; the Schiff base itself has antibacterial activity, and after being combined with gold (III) ions, the Schiff base can stabilize gold atoms and improve the activity of the gold atoms; in vitro antibacterial activity shows that Au6 and Au13 have significant antibacterial advantages on carbapenem and polymyxin-resistant Klebsiella pneumoniae, the minimum inhibitory concentration (MIC) is 10 micromoles, and the antibacterial activity is at least 16 times higher than that of the gold sodium aurothiomalate; in vivo antibacterial activity experiments prove that Au6 and Au13 can reduce mouse skin infection and promote wound healing, and can prolong the survival time of abdominal infection mice, and have important practical application value.

The invention provides an application of echinocystic acid in preparation of a type II 5 alpha-reductase inhibitor product. In-vitro tests prove that echinocystic acid has an obvious inhibition effect on the activity of II type 5 alpha-reductase; experimental testosterone-induced androgenic alopecia mouse models prove that echinocystic acid can promote mouse hair growth and reduce the content of dihydrotestosterone in mouse skin, and can be used for preparing products for treating androgenic alopecia by inhibiting the activity of type II 5alpha-reductase.

The invention discloses a pharmaceutical composition for removing chloasma with a synergistic effect. The pharmaceutical composition comprises ginsenoside Rk3 and hydroxysafflor yellow A which are used as active ingredients, and a pharmaceutically acceptable carrier, and the weight ratio of the ginsenoside Rk3 to the hydroxysafflor yellow A is 1: (0.1-0.5). Experimental results show that the improvement effect of the pharmaceutical composition on the level of tyrosinase in mouse skin is obviously improved compared with that of a single ginsenoside group Rk3 and a single hydroxysafflor yellow A group, the combination index (CI) of the ginsenoside Rk3 and the hydroxysafflor yellow A is smaller than 1, and it is shown that the combined use of the two drugs has a synergistic effect.