The application discloses two peptide segments derived from Cervus elaphus Linnaeus and application of the two peptide segments in promoting wound healing. Amino acid sequences of the two peptide segments are Gly-Ser-Phe-Pro-Trp-Gln-Ala-Lys (SEQ ID NO:1) and His-Ser-Trp-Ala-Val-Pro-Ser-Leu (SEQ ID NO:2) respectively. In-vitro experiments show that the two peptide segments can significantly promote proliferation and migration of human umbilical vein endothelial cells and mouse skin fibroblasts; in-vivo mouse acute wound model experiments prove that the two peptide segments can effectively accelerate the healing process of mouse acute wounds, have excellent biological activity of promoting acute skinwound healing, and have a good application prospect in development of drugs and functional products related to wound repair.