32 results about "Mouse skin" patented technology

The invention relates to the technical field of biological medicines, in particular to a soluble skin-adhering microneedle for delivering an inactivated enterovirus vaccine as well as a preparation method and application of the soluble skin-adhering microneedle. Sodium hyaluronate is adopted as a film forming material to wrap an enterovirus 71 type (EV71)-coxsackie virus A16 (CA16) type bivalent inactivated vaccine antigen, and a needle body layer of the soluble microneedle is formed; the substrate layer is prepared from a pullulan material, so that the microneedle patch with a stable overall structure is formed. The technology provided by the invention is completed in a clean environment, and the vaccine patch which is qualified through sterility detection is obtained. The soluble microneedle disclosed by the invention has good antigen stability under the condition of 2-8 DEG C. In animal experiments, the microneedle is attached to the skin surface of a mouse for skin-adhering immunization, a strong immune response reaction can be induced, good immunogenicity is shown, and a novel, safe and effective vaccine delivery way is provided for prevention and control of the infantile hand-foot-and-mouth disease.

The invention provides lignan, and belongs to the technical field of medicines. According to the invention, a compound shown as a formula I and a compound shown as a formula II are extracted, separated and identified from schisandra chinensis oil for the first time, and the compound shown as the formula I and the compound shown as the formula II are superior to a positive drug group arbutin in the aspect of inhibiting generation of melanin of mouse skin melanoma cells (B16F10); an inflammatory factor release inhibition experiment research shows that the compound shown in the formula I and the compound shown in the formula II have relatively good activity in the aspect of inhibiting LPS-induced mouse mononuclear macrophage RAW264.7 from releasing IL-6 and TNF-alpha; in a skin irritation experiment, the schisandra chinensis non-saponifiable matter containing the compound shown in the formula I and the compound shown in the formula II is non-irritant to the skin of a white rabbit, and it is proved that the compound shown in the formula I and the compound shown in the formula II can be applied to preparation of whitening and anti-inflammatory products. Formula I; formula II.

The invention discloses a high-bionic in-vivo immune microenvironment in-vitro skin model and application, and belongs to the technical field of biological medicine. The preparation method of the in-vitro skin model of the high-bionic in-vivo immune microenvironment comprises the following steps: firstly, adding a culture medium into the lower layer of a six-pore plate, then adding a mouse bone marrow cell suspension, then putting the six-pore plate into a Transwell chamber, flatly laying separated mouse skin on an absorptive gelatin sponge, putting the absorptive gelatin sponge into the Transwell chamber, adding the culture medium into the Transwell chamber, and then putting the mouse skin into the transwell chamber to obtain the in-vitro skin model of the high-bionic in-vivo immune microenvironment. Placing the six-hole plate in a cell incubator for culturing to obtain the in-vitro skin model. According to the in-vitro skin-bone marrow cell co-culture system, an immune environment is provided for the in-vitro skin by introducing bone marrow cells, so that the culture environment of the in-vitro skin is more complete, the difficulties that cell components are simple and the immune environment is deficient at present can be solved, and the in-vitro skin-bone marrow cell co-culture system is more in line with the 3R principle and is high in bionic property and low in cost.

The utility model relates to the technical field of biological experiments, and discloses a simple rat and mouse skin drag hook which comprises a drag hook body, and the drag hook body serves as an integral part of the whole drag hook, serves as a main basic component of surgical operation and is further used for assembling and bearing a lower structural component which the drag hook body belongs to. The skin hooking area is used as a skin hooking function part of the whole retractor body during an operation; the two combination areas are used as parts with a supporting function during storage and placement; the hanging area is used as a fixing part during surgical operation; and a suspension assembly. According to the utility model, in rat or mouse experimental research, a scalpel is used for cutting open the skin surface, an operator pinches the hanging area to penetrate the skin hooking area into the skin to stretch so as to expand space, a needle tube penetrates through the inner side of the hanging area, and the needle tube is inserted into a table top, so that the drag hook body can be fixed, or the drag hook body is fixed by a pull rope; assistants are not needed, the operation space is enlarged, and the operation is more convenient.

The invention discloses an operation table for dissecting rats and mice, and relates to the technical field of mouse dissecting tables, the operation table comprises an experiment assembly and a laparotomy assembly, and the experiment assembly comprises a working plate; the laparotomy assembly comprises a rotating frame, a deformation plate and a clamping plate; the rotating frame is rotationally connected to the working plate through a friction hinge and is used for placing a mouse; during laparotomy and mouse observation, the rotating frame can drive the whole mouse to rotate, and the wound orientation is adjusted; a clamping plate is arranged at one end, far away from the rotating frame, of the deformation plate; the deformation plate is made of a bendable iron material, and the clamping plate is used for clamping and unfolding the epidermis of a mouse; according to the device, an operator can adjust the angle and the distance between the two clamping plates at will through the deformation plate capable of being manually bent, so that the device can be perfectly attached to mice of various body types from small size to large size, the problem that the belly is not fully lifted due to too loose clamping or the chest is pressed due to too tight clamping is solved, and a stable foundation is provided for anatomy operation.

The utility model relates to the technical field of cell collection, and discloses a mouse skin cell collection brush which comprises a shell, a sliding block is slidably connected to the interior of the shell, a rotating block is rotatably connected to the rear end of the sliding block, a connecting column is fixedly connected to the top end of the rotating block, a fixed block is slidably connected to the interior of the sliding block, and the connecting column is fixedly connected to the top end of the rotating block. The outer portion of the connecting column is slidably connected to the outer portion of the shell, a brush is fixedly connected to the front end of the fixing block, a limiting assembly used for limiting is arranged outside the connecting column, a dismounting and mounting assembly used for dismounting and mounting is arranged inside the rotating block, and the limiting assembly comprises a sliding block. The interior of the sliding block is connected to the exterior of the connecting column in a sliding mode. According to the utility model, the length of the brush can be adjusted, so that the applicability and the collection efficiency of the collection work aiming at different parts are greatly improved, and the whole collection process is more scientific, convenient and efficient.

The invention discloses an integrated experimental device for mouse skin photoaging modeling, and relates to the technical field of skin photoaging modeling. The mouse fixing device comprises a fixing main plate and a plurality of semicircular fixing cylinders. The fixed main board is erected in the experiment box body and is provided with a plurality of groups of fixed cylinder slots; the semicircular fixed cylinder is arranged in the fixed cylinder slot in a pluggable manner; a cylinder body of the semicircular fixing cylinder is provided with a fixing hole through which an elastic binding piece penetrates, and the head end of the semicircular fixing cylinder is provided with an integrally-formed arc-shaped baffle used for shielding the eyes of the mouse during ultraviolet irradiation. The stress intervention system is integrated in the experiment box body and comprises a blue light phototaxis sedation module and an olfaction pacification spraying module; the blue light phototaxis sedation module comprises at least one group of blue light sources arranged in the box body and is used for inducing mice to settle; the olfaction pacifying spraying module comprises an atomizer and a spraying outlet and is used for releasing pacifying aerial fog into the box body, multiple functions of animal fixing, ultraviolet irradiation, eye protection and stress control are synchronously completed in a single device, and the standardization degree and data reliability of light aging modeling are remarkably improved.

The invention discloses a construction method of a sensitive skin animal model, and belongs to the technical field of animal model construction. According to the method disclosed by the invention, the sensitive skin animal model is constructed by smearing the lauryl sodium sulfate on the back skin of the mouse after hair removal in combination with veratrine or capsaicin, so that physiological indexes of human sensitive skin can be simulated; key pathological processes of human sensitive skin can be simulated by detecting key pathological indexes such as skin barrier damage, nerve and blood vessel high reactivity, immune inflammation and the like, natural pathophysiological processes of human sensitive skin can be simulated, and pathogenesis of sensitive skin is basically covered; barrier destruction of mouse skin, high reactivity of nerve and blood vessels and inflammatory response are induced, and clinical practice is met. In addition, the method is good in repeatability, high in safety and easy and convenient to operate, sensitive skin medicine screening and personalized treatment scheme development can be promoted, the research and development period of sensitive skin related treatment schemes is shortened, and the application prospect is good.

The invention discloses a lactobacillus rhamnosus LR-C111 contributing to skin aging resistance and application of the lactobacillus rhamnosus LR-C111. The lactobacillus rhamnosus LR-C111 is classified and named as lactobacillus rhamnosus, the preservation number is CCTCC NO: M 20251647, the preservation unit is China Center for Type Culture Collection, and the preservation time is July 21, 2025. The lactobacillus rhamnosus LR-C111 is named as lactobacillus rhamnosus LR-C111. The lactobacillus rhamnosus LR-C111 can enhance the antioxidant enzyme activity of mouse skin and reduce the generation of oxygen free radicals by inhibiting the expression of MMP-1 and MMP-9 pathways, so as to inhibit the content decomposition of endogenous components in the skin and reduce the loss of skin collagen and the degradation of ECM (extracellular matrix), thereby delaying the skin aging. The composition can be used for preventing, treating and / or improving skin elasticity reduction, skin wrinkles and other symptoms caused by skin photoaging caused by ultraviolet rays.

The invention relates to application of bornyl acetate and an external gel preparation thereof in preparation of a medicine for relieving or treating chronic pruritus. Compared with the prior art, the bornyl acetate separated from volatile components of the folium artemisiae argyi supercritical extract has remarkable effects of inhibiting mast cell degranulation and inhibiting expression of pruritus-related factors at a cellular level and an animal level, and can be used for remarkably relieving mouse skin injury caused by DNCB; the polypeptide has the advantages of good safety, high selection index and the like, can be used for developing drugs for relieving and / or treating chronic itching and expanding treatment schemes of chronic itching, and has wide application prospects and important clinical significance.

The application discloses synthesis of a novel Schiff base gold (III) compound and antibacterial application thereof, and belongs to the technical field of medicine preparation; gold sodium aurothiomalate has significant antibacterial activity, but the Au-S bond thereof is easily broken by intracellular reducing thiol, and the gold sodium aurothiomalate is metabolized before reaching a target point to produce toxic side effects; based on the gold sodium aurothiomalate, developing a gold compound with stronger stability is one of strategies for overcoming drug-resistant bacteria; the Schiff base itself has antibacterial activity, and after being combined with gold (III) ions, the Schiff base can stabilize gold atoms and improve the activity of the gold atoms; in vitro antibacterial activity shows that Au6 and Au13 have significant antibacterial advantages on carbapenem and polymyxin-resistant Klebsiella pneumoniae, the minimum inhibitory concentration (MIC) is 10 micromoles, and the antibacterial activity is at least 16 times higher than that of the gold sodium aurothiomalate; in vivo antibacterial activity experiments prove that Au6 and Au13 can reduce mouse skin infection and promote wound healing, and can prolong the survival time of abdominal infection mice, and have important practical application value.

The invention provides an application of echinocystic acid in preparation of a type II 5 alpha-reductase inhibitor product. In-vitro tests prove that echinocystic acid has an obvious inhibition effect on the activity of II type 5 alpha-reductase; experimental testosterone-induced androgenic alopecia mouse models prove that echinocystic acid can promote mouse hair growth and reduce the content of dihydrotestosterone in mouse skin, and can be used for preparing products for treating androgenic alopecia by inhibiting the activity of type II 5alpha-reductase.

The invention discloses a method for preparing rhamnolipid through fermentation and application, and belongs to the technical field of fermentation. The dry banana powder and the dry purple cabbage powder are mixed and jointly used as the culture medium additive, the prepared composite culture medium is used for pseudomonas aeruginosa fermentation, and compared with a single culture medium additive or no culture medium additive, the OD value in obtained fermentation liquor is larger, and the composite culture medium is more suitable for fermentation preparation of rhamnolipid. The obtained rhamnolipid is used for cell and animal experiments, and a skin aging cell and mouse skin light aging model is established by using a 311 nm UVB ultraviolet lamp. Tests prove that after the rhamnolipid is used, harmful active oxygen of cells is reduced, surface skin thickening caused by skin photoaging is relieved, and it is proved that the rhamnolipid not only can relieve the skin photoaging symptom caused by long-term ultraviolet irradiation, but also can prevent long-term stimulation of ultraviolet to mouse skin.

The invention relates to an immortalized cell line of human dermal papilla cells as well as a construction method and application of the immortalized cell line. The human dermal papilla cells are named as DYZ-20, the immortalized cell line is preserved in the China Center for Type Culture Collection on July 2, 2025, survival is detected, and the preservation number is CCTCC NO: C2025204. The immortalized cell line of the human dermal papilla cells can normally express alkaline phosphatase and a dermal papilla characteristic gene, and hair can grow after the immortalized cell line is transplanted into mouse skin.

The invention relates to the field of polypeptides, in particular to a polypeptide and application thereof in the aspects of tissue repair, cell scaffolds and cosmetology. The amino acid sequence of the polypeptide is Asn-Ala-Ser-Glu-Leu-Ser-Arg-Glu-Met-Ala-Thr-Gln-Leu-Phe-Gly, the aperture and the strength of the hydrogel prepared from the polypeptide meet the application requirements, and a foundation is laid for application of the hydrogel to the aspects of cell scaffolds, drug carriers and the like. Besides, the polypeptide can effectively promote fibroblast proliferation, has a good inhibition effect on melanin, can obviously promote healing of mouse skin wounds and scalds, has obvious anti-aging, anti-wrinkle, whitening, wound and scald repairing effects and the like, is low in toxic and side effects, has a good application prospect, and is worthy of popularization and application. The compound can be used for preparing drugs, cosmetics or medical instruments for promoting tissue repair, cell scaffolds or skin beautification.

The utility model provides a bacterial liquid spraying device for manufacturing a mouse skin bacterial infection model, which comprises a fixed cylinder and two support rods, a through groove is arranged at the top of the fixed cylinder, a mounting groove is arranged at the front end of the through groove at the top of the fixed cylinder, a fixing mechanism is arranged in the fixed cylinder, sliding grooves are arranged at the top of the fixed cylinder on both sides of the through groove, and the sliding grooves are communicated with the through groove. Mounting plates are fixedly connected to the two ends of the top of the fixed cylinder, a motor is fixedly mounted on one side of the mounting plate at one end, a moving mechanism is arranged between the two mounting plates, and limiting mechanisms are arranged on the portions, on the two sides of the moving mechanism, between the two mounting plates. And then a moving block is driven to move, so that the moving block drives an adjusting plate below to move from the tail of the nude mouse to the head of the nude mouse, and uniform spraying is performed on the skin of the nude mouse through a through groove by utilizing a bacterial liquid spray head, so that the accuracy and repeatability of a research result are better, and the biological safety is better guaranteed.

The invention relates to the technical field of medicine, in particular to application of a compound in treatment of psoriasis, the compound is an AMPK activator Aldometanib, and the chemical structural formula of the compound is as shown in Figure 1. The research result shows that the Aldometanib can effectively relieve the psoriasis-like phenotype of mice, the erythema of the mice is converted from bright red to light red, the area is reduced, silver-white scales attached to the skin surface are thinned, and the psoriasis area and severity index (PASI) score is remarkably reduced. The mouse back skin lesion tissue is subjected to HE dyeing, the visible epidermis thickness is recovered to be close to the normal level through microscopic observation, inflammatory cell infiltration lesions on the superficial layer of dermis and around blood vessels are reduced, and the number is remarkably reduced. A mouse serum ELISA detection result shows that the expression of pro-inflammatory cytokines TNF alpha, IL-1beta and IL-6 is reduced. The Aldometanib disclosed by the invention is remarkable in anti-psoriasis effect, safe and non-toxic, and is expected to become a novel therapeutic drug for psoriasis.

The utility model relates to the technical field of sample boxes, and discloses a mouse skin sample constant-temperature and constant-humidity storage box which comprises a shell, a constant-temperature and constant-humidity mechanism comprises a ventilation block, the ventilation block is fixedly connected in the shell, a fan is fixedly connected in the ventilation block, a filtering assembly is fixedly connected in the ventilation block, and the filtering assembly is fixedly connected in the shell. And a heating pipe is fixedly connected to the interior of the ventilation block, a water flow heater is fixedly connected to the bottom of the heating pipe, and an atomizing nozzle is fixedly connected to the top of the heating pipe. According to the utility model, the temperature and humidity sensor observes the internal humidity and temperature of the device, when the requirements are not met, the fan pumps external air, the external air passes through the ventilation block, in the process, the air passes through the kapok block to filter dust and impurities in the air, and then the air is heated through the heating pipe; water flow in the water storage tank flows into the water flow heater through the water conveying pipe to be heated.

The invention provides application of pinolenic acid methyl ester in preparation of anti-inflammatory and / or whitening products and an extraction method of the pinolenic acid methyl ester, and belongs to the technical field of biological medicine. Purified Pinolenic acid methyl ester is extracted from Korean pine seed oil, and the Pinolenic acid methyl ester has excellent anti-inflammatory and whitening activity. An inflammatory factor release inhibition experiment shows that the methyl pinolenic acid ester can effectively inhibit LPS-induced RAW264.7 cell NO secretion and inflammatory mediator IL-6 and TNF-alpha production, the activity of the methyl pinolenic acid ester is superior to that of pinolenic acid, and even part of the activity of the methyl pinolenic acid ester is superior to that of potent anti-inflammatory glucocorticoid dexamethasone. Whitening activity experiments show that the methyl pinolenic acid ester also has better activity in the aspect of inhibiting the generation of melanin of mouse skin melanoma cells (B16F10). In a skin irritation experiment, the Korean pine seed oil non-saponifiable matter containing the pinolenic acid methyl ester has no irritation to the skin of the white rabbits. Therefore, the pinolenic acid methyl ester has certain potential in the aspect of being developed into whitening and anti-inflammatory products.

The application discloses lactobacillus johnsonii capable of orally assisting in relieving vaginitis and improving skin aging, and belongs to the technical field of microorganisms.The lactobacillus johnsonii is separated from vaginal secretions of healthy women, has the effects of repairing immune disorders caused by vaginitis and skin aging of mice with vaginitis, and is specifically reflected in the following aspects: reducing the content of IL-1beta in cell supernatant; improving inflammatory reactions of mouse vaginas; reducing the expression of TLR-4 mRNA and the level of TNF-alpha in vaginal tissues; enhancing the anti-aging ability of mouse skin; reducing the expression of MAPK / AP-1 / MMP-1 mRNA, increasing the content of Col I and Col III in skin; improving pathological characteristics of mouse vaginas and reducing the load of pathogenic bacteria.Therefore, the lactobacillus johnsonii has great application prospects in products for improving and repairing inflammatory damage caused by vaginitis and skin health problems of mice with vaginitis.

The present application belongs to the field of microbial technology, and particularly relates to a plant lacto-plantarum extracellular vesicle and application thereof. Specifically, the plant lacto-plantarum extracellular vesicle can promote HaCaT cells to generate ceramide, up-regulate the expression of key genes in the ceramide synthesis pathway of skin, and restore the skin barrier. The plant lacto-plantarum extracellular vesicle can reduce the expression of pro-inflammatory factors in a cell inflammation model, and has good anti-inflammatory effect. The mouse skin damage model verification shows that the EVs have good skin penetration ability, can significantly reduce the skin damage score, relieve skin itching, improve the skin water content, and reduce the expression of pro-inflammatory factors and the expression of itching-related cytokines in the skin tissue.

This invention provides a lignan, belonging to the field of pharmaceutical technology. This invention is the first to extract, isolate, and identify compounds of formula I and formula II from Schisandra chinensis oil. Both compounds of formula I and formula II are superior to the positive control group arbutin in inhibiting melanin production in mouse skin melanoma cells (B16F10). Through experiments inhibiting the release of inflammatory factors, it was found that compounds of formula I and formula II have good activity in inhibiting the release of IL-6 and TNF-α from LPS-induced mouse monocyte-macrophage RAW264.7 cells. In skin irritation experiments, the unsaponifiables of Schisandra chinensis, including compounds of formula I and formula II, were non-irritating to rabbit skin, proving that compounds of formula I and formula II can be used to prepare whitening and anti-inflammatory products. Formula I; Formula II.

The mouse skin suture auxiliary device comprises a first clamp body, a second clamp body and a plurality of tweezers teeth, the first clamp body is hinged to the second clamp body so that the first clamp body and the second clamp body can rotate around the hinge point, and the tweezers teeth are arranged on the first clamp body and the second clamp body. The tweezer teeth are arranged at one ends of the bottoms of the first clamp body and the second clamp body at equal intervals; the skin on two cut sides is pulled oppositely to be aligned, then one ends of the bottoms of the first clip body and the second clip body are opened, the tweezers teeth on the first clip body and the second clip body are aligned to two sides of the skin surface, then the one ends of the bottoms of the first clip body and the second clip body are closed, and the skin is fixed by the tweezers teeth. At the moment, sutures can be inserted into gaps between the adjacent tweezer teeth to achieve preliminary suturing, after preliminary suturing, fixation of the skin by the first clamp body and the second clamp body is removed, then the sutures are tightened, and then the skin of the mouse is sutured by a single person.

The invention provides a composition containing stem cell exosomes and application of the composition in medicines or cosmetics. Furthermore, the composition disclosed by the invention also contains whitening active peptide separated from the hairtail, and the active peptide can effectively inhibit the activity of tyrosinase. After the active peptide and the exosome are independently used or combined, mouse aging can be effectively resisted, the content of mouse skin hydroxyproline is increased, and the active peptide has a good application prospect.

The invention belongs to the technical field of skin care products, and particularly relates to neohesperidin nano-liposome, a sunscreen emulsion and a preparation method and application of the neohesperidin nano-liposome and the sunscreen emulsion. The neohesperidin nano-liposome provided by the invention is formed by wrapping neohesperidin in a lipid membrane, and in-vitro experiments prove that the emulsion containing the neohesperidin nano-liposome can significantly improve UVB-induced mouse skin light injury, and the skin presents a small amount of injury traces; skin swelling and corium layer thickening induced by UVB irradiation are reduced, corium collagenous fiber reduction caused by UVB irradiation is remarkably improved, and the relative content of collagenous fibers after neohesperidin acts is remarkably increased.

The present application relates to specimen implantation device technical field, especially in a kind of PDX model tumor specimen implantation device;Operator first mouse is fixed on operation table, then the device is placed on operation table and close to mouse;Rotating implantation cylinder to implantation cylinder is aligned to the position of mouse to be implanted, the position and angle of implantation cylinder are then locked by first locking component, then implantation cylinder and piston rod are simultaneously pushed to embed sharp head into the position of mouse to be implanted;During pushing process, sharp head will pierce mouse skin to form wound, and simultaneously drive implantation cylinder to extend into mouse body;Piston rod is extracted, then sharp head on piston head is unscrewed, and plug screw is installed on piston head, then piston head is re-embedded into implantation cylinder, specimen is placed into implantation cylinder through inlet, and then piston rod is manually pushed, so as to implant specimen into mouse body;After implantation is completed, implantation cylinder is pulled out from mouse body;Whole operation process is safer, more convenient and efficient.

The invention discloses a Pucurbita pepo polypeptide Nacrein-LP20 capable of resisting skin photoaging as well as preparation and application of the Pucurbita pepo polypeptide Nacrein-LP20, and belongs to the technical field of biological medicines. An in-vitro experiment shows that Nacrein-LP20 has a remarkable protection effect on UVB-induced human immortalized keratinocyte injury, and can be used for dose-dependently improving the cell activity, inhibiting the excessive generation of reactive oxygen in cells, enhancing the activity of superoxide dismutase, catalase and glutathione peroxidase, reducing the content of malondialdehyde and the release of lactic dehydrogenase, and inhibiting the damage of UVB-induced human immortalized keratinocyte injury to the human immortalized keratinocyte injury to the human immortalized keratinocyte injury. And cell migration and repair are promoted. In-vivo experiments prove that in a UVB-induced mouse skin photoaging model, photoaging apparent symptoms such as skin erythema, skin thickening and wrinkles can be effectively improved by locally smearing the Nacrein-LP20, epidermal hyperplasia is relieved, and synthesis and ordered arrangement of corium layer collagenous fibers are promoted.

The invention belongs to the technical field of medical materials, and particularly relates to a piezoelectric material for wound dressing and a preparation method of the piezoelectric material. The preparation raw materials comprise silk fibroin, PHBV, barium titanate and a dispersion medium. The piezoelectric material is excellent in cell compatibility, capable of promoting cell proliferation and migration, controllable in degradation and matched with a wound cycle, and the dynamic change of mechanical and piezoelectric properties adapts to the requirements of each stage of wound repair; in addition, the piezoelectric material can remarkably accelerate healing of mouse skin wounds, reduce scars and promote regeneration and remodeling of skin tissues, and related genes and signal channels are regulated and controlled through in-situ electrical stimulation, so that the wound microenvironment is optimized.

The invention discloses an application of a TEAD inhibitor in preparation of a medicine for treating skin fibrosis, and experiments prove that a TEAD palmitoylation inhibitor MGH-CP1 shows remarkable anti-fibrosis activity in vitro and in vivo, and can effectively inhibit fibroblast activation and ECM deposition induced by TGF-beta or hypoxia. And the pharmaceutical composition has a good curative effect and safety in a bleomycin-induced mouse skin fibrosis model. According to the application of the TEAD inhibitor, a new treatment target and a new treatment strategy are provided for various chronic fibrosis diseases related to skin fibrosis, and the TEAD inhibitor is expected to be clinically applied to improve the treatment effect of the chronic fibrosis diseases represented by systemic sclerosis and keloid, increase the survival rate of patients and prolong the lifetime of the patients.

The invention discloses an application of a CK2 inhibitor CX4945 in preparation of medicines for treating skin inflammations such as psoriasis and the like. The CK2 inhibitor CX4945 ointment provided by the invention can be used for remarkably reducing the pathological injury of the skin of a psoriasis mouse and reducing the neutrophil infiltration of the dermis layer of the skin. The medicine can be prepared into external ointment, is locally applied for use, is convenient to use, has no obvious side effect, is high in patient compliance, and can be prepared into a psoriasis treatment medicine.