59 results about "Chemotaxis" patented technology

The application discloses a sludge biological drying multi-target proteinase regulation method based on bacterial chemotaxis, relates to the technical field of sludge resource disposal, and comprises the following steps: S1, constructing a microfluidic detection system based on the chemotaxis of thermophilic bacteria, and monitoring and quantifying the metabolic preference degree of the thermophilic bacteria to different amino acids in a sludge biological drying process; S2, selecting and proportioning proteinases with a targeted degradation function according to the metabolic preference degree of the thermophilic bacteria; and S3, decomposing refractory proteins into specific amino acids preferred by the thermophilic bacteria through multi-target regulation of the proteinases. The application optimizes the metabolic environment of the thermophilic bacteria to improve the utilization rate of protein organic matter and accelerate the sludge biological drying process.

The invention belongs to the field of plant protection, and discloses a method for inducing bemisia tabaci by a persicaria orientalis volatile matter and a related application of the persicaria orientalis volatile matter to the bemisia tabaci. A Y-type olfactometer behavior experiment shows that the bemisia tabaci has a remarkable chemotactic reaction on the red-knees herb, and the preference of the bemisia tabaci is obviously higher than that of a common host tomato. Furthermore, solid phase microextraction (SPME) is combined with gas chromatography-mass spectrometry (GC-MS) to analyze the smell components of the polygonum hydropiper, and various volatile substances possibly participating in chemotaxis are screened out. The method can be used for developing a plant source smell attractant and improving the pest monitoring and green prevention and control efficiency.

The invention relates to a collaboration and grouping method for swarm robots inspired by bacterial tropism, which comprises the following steps of: establishing a fitness function of a robot according to position information of neighbors in a local sensing range of an individual robot on the basis of a bacterial food source sensing mechanism; based on the fitness function and the bacterial tropism mechanism, determining the basic behavior state of the robot, and obtaining a motion switching result about whether behavior state switching occurs or not; correcting the motion state of the robot and determining the rotation angle of the robot based on the switching result; wherein the rotation angle is used for controlling the swarm robots to keep the same kind of subgroups agglomerated, keep away from different kinds of groups and search for other same kind of individuals, gathering in a certain area of the environment, and grouping the swarm robots. According to the method, the robot behavior state is determined by analyzing the bacterial tropism mechanism, the behavior state switching mechanism is constructed, the rotation angle is corrected, the swarm robot grouping effect is achieved, swarm robot distributed cooperative grouping is achieved, and the grouping efficiency is improved.

The invention relates to the technical field of microbial preparation optimization and intelligent algorithm crossing, and particularly discloses a foraging algorithm for coupling dominant functional bacteria of targeted biological bacteria and host original flora, and the foraging algorithm simulates a coupled symbiotic system of the targeted biological bacteria and the host original flora into a flora society. Each to-be-optimized single-function bacterial strain is regarded as a'bacterial 'individual in the algorithm, the comprehensive effect of a flora on a specific target is used as a fitness function, global optimization is carried out through an improved chaos optimization bacterial foraging algorithm, and through the steps of flora proportion initialization, chemotaxis operation, breeding operation, migration operation and the like, the optimal bacterial foraging algorithm is obtained. The method provided by the invention solves the problems of complex work, long period and difficulty in obtaining a globally optimal solution in the traditional'trial-and-error method 'for preparing the composite flora, and provides core algorithm support for quickly, accurately and intelligently developing an efficient composite microbial preparation.

The invention belongs to the technical field of biology, and particularly relates to application of a herba artemisiae scopariae aqueous extract in enhancing liver chemotaxis of adipose-derived mesenchymal stem cells. In order to provide a way for enhancing the liver chemotaxis of the adipose-derived stem cells, the capillary artemisia aqueous extract is used for improving the expression level of chemotactic factors of the adipose-derived stem cells in vitro and intervening the proliferation, migration and chemotaxis capabilities of the adipose-derived stem cells to liver cells, the concentration of the capillary artemisia aqueous extract is 0.5 mg / mL to 1 mg / mL, the chemotaxis time is 24 h to 48 h, and the capillary artemisia aqueous extract is used for enhancing the liver chemotaxis of the adipose-derived stem cells. And the chemotactic factors are CCR1 and CXCR4.

The invention discloses an application of a CD177 targeted membrane modified liposome in preparation of a medicine for preventing and / or treating psoriasis. The structure of the CD177 targeting membrane modified liposome comprises a PADI4 inhibitor loaded liposome, a targeting recognition layer formed by coupling CD177 targeting peptide on the surface of the liposome, and a bionic functional layer formed by coating a natural neutrophile granulocyte membrane on the outermost layer. Based on the core pathological mechanism of 'CD177 + neutrophil-NETs' of psoriasis, the inflammation chemotaxis / cell factor neutralization function of a neutrophil membrane, the precise targeting function of CD177 peptide and the NETs inhibition function of a PADI4 inhibitor are creatively and organically integrated, and the constructed GNLC achieves the synergistic treatment effect of 'targeting enrichment-mechanism blocking-microenvironment remodeling'; meanwhile, excellent stability and safety are achieved, and a new precise treatment strategy far better than that in the prior art is provided for psoriasis.

The invention discloses an indoline derivative and application thereof. According to the invention, the regulation effect of the derivative on the chemotaxis and secretion functions of ILC3 is evaluated; it is found that the derivative has a promoting effect on chemotaxis of ILC3 to an inflammatory site and up-regulation of IL-22 expression, has very remarkable effects on mouse ulcerative colitis (UC) induced by DSS, mouse Crohn disease (CD) induced by TNBS, acute lung injury induced by lipopolysaccharide (LPS), mouse psoriasis induced by imiquimod and other diseases in vivo, and is superior to existing clinical treatment drugs. Good development and application prospects are realized.

The invention discloses a macrophage-platelet targeting delivery system carrying genes and mitochondria as well as a preparation method and application of the macrophage-platelet targeting delivery system. The platelet loaded with the PPAR gamma gene nano-composite is covalently linked to the surface of macrophage through a click chemical reaction, and a novel cell composite carrier is constructed. According to the system, the natural chemotaxis of macrophages to an injured part is fully utilized to realize precise targeting, and PPAR gamma genes and functional mitochondria are synchronously released through platelet activation in an injured microenvironment, so that the energy metabolism and lipid homeostasis of the macrophages are cooperatively regulated and controlled, the cellular burial function of the macrophages is effectively recovered, and tissue repair and regeneration are promoted. Experimental results show that the delivery system is reliable in preparation method and high in targeting efficiency, axon regeneration, myelin sheath repair and motor function recovery can be remarkably promoted in a spinal cord injury model, and a brand-new synergistic treatment strategy is provided for tissue injury repair.

The application discloses a mobile edge computing task offloading method based on a bacterial foraging algorithm, and steps of the method comprise the following steps: S1, constructing a many-to-many edge computing offloading model; S2, establishing a time delay model and an energy consumption model of an edge server and a mobile device; S3, taking the longest time delay on the edge server and the mobile device as the total time delay of the system, taking the total energy consumption as the system energy consumption, obtaining a system optimization total target by weighting, obtaining the system optimal target by using an improved bacterial foraging algorithm, and obtaining an optimal offloading result; S4, modifying a three-layer loop nested structure of the bacterial foraging algorithm, changing the bacterial foraging algorithm into a single-layer loop structure in parallel with chemotaxis and migration, and using the improved bacterial foraging algorithm to continuously iteratively update the bacterial position and obtain the best offloading strategy and the system optimization target. The method can ensure that all the mobile devices in the system can reasonably offload the computing task to the edge server or process the computing task on the mobile device, so that the system time delay and the energy consumption are reduced.

The invention relates to a targeted temperature-sensitive liposome loaded with hydrophilic and hydrophobic drugs, and a preparation method and application thereof, and belongs to the technical field of drug delivery. The invention provides a temperature-sensitive liposome simultaneously loading hydrophilic and hydrophobic drugs. The temperature-sensitive liposome comprises a liposome as a carrier, a hydrophobic anti-cancer drug and a light-sensitive material are loaded between the bimolecular layers of the liposome; a hydrophilic anti-cancer drug doxorubicin hydrochloride is wrapped in the carrier. The invention further provides a delivery system wrapped by an antibody, and the antibody is further modified on the surface of the temperature-sensitive liposome. The antibody-coated delivery system can actively target the activated neutrophil, and the chemotaxis of the tumor microenvironment to the neutrophil is utilized to realize the efficient enrichment of the drug at the tumor site, thereby achieving the targeted therapy and controllable release of the tumor.

This invention discloses a microfluidic chip for three-stage non-destructive sperm screening and its usage method. The chip includes a substrate, a sample inlet channel layer, a sample chamber layer, a recovery chamber layer, and a screening filter membrane. The sample inlet channel layer has an inlet channel and an overflow channel. The sample chamber layer has an annular sample chamber, an inlet orifice, and an overflow orifice. The recovery chamber layer is divided into an outer recovery chamber and an inner recovery chamber by a dike. An inducing agent patch is located in the center of the inner recovery chamber. The screening filter membrane is sandwiched between the sample chamber layer and the recovery chamber layer. In use, after injecting a sperm sample, initial motility screening is achieved through the screening filter membrane. Secondary chemotactic screening is completed through an inducing agent concentration gradient. Tertiary adaptive screening for swimming direction is completed using the chemotactic channel. Finally, high-quality sperm are obtained from the inner recovery chamber. This invention achieves multi-parameter graded screening, effectively avoiding sperm damage, improving sperm recovery quality and screening efficiency, and is applicable to various assisted reproductive technologies, helping to improve conception success rates.

The application discloses a di(2-ethylhexyl) phthalate degrading bacterium, a bacterial composition and application thereof. The degrading bacterium is named Sphingomonas parapaucimobilis GHQ28 and Stenotrophomonas maltophilia GHQ31. Research finds that GHQ28 and GHQ31 have chemotaxis to DEHP and downstream metabolites thereof, and there is a synergistic interaction between the two bacteria; the growth and DEHP degradation speed of the two strains under co-culture conditions are faster than that under single culture conditions. The bacterial composition composed of the two strains has a highest degradation rate of 99.2±0.59% to 400mg / L DEHP within 72h, compared with single culture, the total biomass is increased by 1.4-50.3 times, and the DEHP degradation rate is increased by 1.07-58.82 times. The application provides a new technical means for remediation of DEHP contaminated soil or water.

The invention provides a preparation process of a soothing and repairing type mask, and belongs to the technical field of cosmetics, an intelligent response type cell bionic delivery system is adopted, macrophage membrane vesicles serve as a carrier, a composite repairing kernel, epicatechin gallate, a neuropeptide P substance antagonist and idebenone are wrapped, and by means of the inflammation chemotaxis of a macrophage membrane, the soothing and repairing type mask is prepared. According to the present invention, the active component can actively target the inflammation region, the phase change release core is provided in the weakly acidic microenvironment with the pH value of 5.0-6.0, the targeting property and the permeation efficiency of the active component can be improved, the bottleneck of the traditional passive diffusion can be broken through, and compared with the conventional liposome or nanoparticle, the active component can be accurately delivered to the inflammation core region, the permeation rate can be improved by several times, and the carrier can directly reach the problem core region; according to the method, the utilization rate of effective components is greatly increased, a micro-ecology-immunity-nerve-physical barrier multi-dimensional and systematic synergistic repair network is constructed, full-path fundamental repair from immediate relieving to root strengthening is achieved, and immediate relieving and long-term barrier repair effects are provided.

The invention relates to an inflammatory environment induced overexpression 1L-10 engineered macrophage and a construction method and application thereof, and belongs to the field of biomedicine.The construction method includes the steps that a recombinant plasmid for expressing IL-10 is constructed with a genetic engineering method, and an RAW264.7 macrophage is jointly transfected with the constructed recombinant plasmid for expressing IL-10 and a Cas9 plasmid, so that the engineering macrophage with the inflammatory environment induced overexpression 1L-10 is obtained, and the engineering macrophage with the inflammatory environment induced overexpression 1L-10 is obtained. According to the method, the IL-10 gene is used for replacing the position of the IL-1 gene in the RAW264.7 macrophage, so that the RAW264.7 macrophage can express IL-10 under the induction of an inflammatory environment, the constructed engineered macrophage is injected into the abdominal cavity of a colitis model mouse in an intraperitoneal injection manner, the macrophage is positioned to the colitis disease part and secretes an anti-inflammatory cytokine IL-10 due to the inflammatory chemotaxis of the macrophage, and the anti-inflammatory cytokine IL-10 is obtained. The effect of relieving intestinal inflammation is achieved. According to the invention, the development of ulcerative colitis treatment technology is facilitated, and the application of a macrophage-based cell treatment method in treatment of digestive system diseases is promoted.

The invention discloses an application of a Pik3cb inhibitor in treatment of Sjogren's disease. Experiments prove that the Pik3cb inhibitor TGX-221 can reduce the number, activation and chemotaxis of NOD mouse B cells of a Sjogren's disease model mouse, plasma cell differentiation and antibody secretion, improve the immune microenvironment of the submaxillary gland of the Sjogren's disease model mouse, reduce the infiltration degree of submaxillary gland lymphocytes and promote the salivary secretion function; and after the Pik3cb is over-expressed, the symptom and pathology of the Sjogren's disease are aggravated. Therefore, the Pik3cb inhibitor can be used for treating the Sjogren's disease. The invention provides a new thought for research and development of medicines for treating the Sjogren's disease, and has important application value.

The invention provides a method for enhancing the tumor homing ability of TIL cells, and belongs to the technical field of biological medicine. The invention provides a method for effectively enhancing the homing ability of TIL cells, so that the chemotactic and enrichment potential of engineered TIL cell products to tumor sites is remarkably improved. The TIL cell preparation prepared on the basis of the method has higher tumor targeting performance, and a new solution is provided for overcoming the bottleneck that effector cells'reach 'tumor parts in an existing cell therapy. The effectiveness of single cell therapy is expected to be directly improved, and better cell products can be provided for synergistic combination with other therapies such as radiotherapy and chemotherapy, so that wider and more effective treatment choices are brought to patients with solid tumors.

The application provides a culture method and a verification method for evaluating a model of circadian rhythm, and belongs to the field of disorder model establishment and active substance function evaluation. By culturing nematodes with normal circadian rhythm and nematodes with circadian rhythm disorder, a model for evaluating circadian rhythm is established. The method is simple and easy to control, can more accurately simulate the influence of environmental factors on circadian rhythm, and lays a foundation for establishing a stable and repeatable evaluation model of circadian rhythm disorder. Whether the circadian rhythm of the nematodes is normal or not is effectively verified by the following method: a test plate is prepared, the nematodes with normal circadian rhythm or the nematodes with circadian rhythm disorder are placed in a test area; a fixed volume of an inducer is added at a fixed position, and the test plate is covered; nematode numbers in different areas of multiple test plates are counted at intervals of a fixed time; a chemotaxis index is calculated to verify the circadian rhythm.

This invention proposes a method for preparing a neutrophil membrane-like biomimetic nanoplatform, characterized by the following steps: Step 1: Synthesizing a 4-octyl itaconic acid-retinol prodrug (OR); Step 2: Preparing ORNPs; Step 3: Extracting dHL-60 cell membranes; Step 4: Assembling M@ORNPs to obtain core-shell structured M@ORNPs nanoparticles with a hydration kinetic diameter of approximately 108.2 nm, which constitute the biomimetic nanoplatform. The biomimetic nanoplatform possesses active chemotaxis and immune escape properties, enabling precise delivery of 4-octyl itaconic acid-retinol in sepsis-associated acute lung injury.

The application provides a kind of lipopeptide compound PW-1 and its preparation method and application in preventing and controlling bacterial wilt, belong to biological pesticide technical field;The PW-1 is prepared by fermentation, solvent extraction, acidification and reverse phase chromatography from Paenibacillus polymyxa (P. Paenibacillus polymyxa ) strain DW-7A;The core of the compound is that it plays a role through "anti-toxicity" mechanism, at the concentration without inhibiting the growth of Pseudomonas solanacearum (P. Ralstonia solanacearum ), can specifically interfere with the biosynthesis of pathogenic bacteria flagellum, cause its motility, chemotaxis and early infection ability to decrease significantly;Pot experiment proves that the relative control efficiency of PW-1 to tobacco bacterial wilt reaches 62.2%, and has no effect on the growth of soil bacillus at the concentration of up to 1000 μg / mL, shows efficient, targeted and environment compatible characteristics;The application provides a new candidate compound and key technical support for the development of green pesticide targeting flagellum.

This invention belongs to the field of pharmaceutical technology and specifically discloses the use of CL in a drug for the treatment and prevention of Alzheimer's disease and Parkinson's disease. Studies have demonstrated that CL can significantly improve the motility and chemotaxis of Caenorhabditis elegans with Alzheimer's disease, significantly extend the lifespan of Caenorhabditis elegans with Alzheimer's disease, and significantly restore damaged neurons in Caenorhabditis elegans with Parkinson's disease, suggesting that CL has the potential to be used in the development of therapeutics for neurodegenerative diseases.

According to the engineered carrier bacterium mediated T cell tumor targeted collaborative treatment system, through delivery of modified carrier bacteria, immunomodulatory molecules are loaded to the carrier bacteria through covalent bonds and / or non-covalent bonds by utilizing natural chemotaxis of the carrier bacteria to a tumor immune microenvironment, and immunomodulatory molecule synergistic carrier bacteria are constructed; the engineering bacteria and CAR-T cells are coupled through covalent bonds and / or non-covalent bonds to form a CAR-T-bacteria chimera. The chimera realizes CAR-T cell tumor enrichment by virtue of the tumor targeting property of carrier bacteria; meanwhile, the immune microenvironment is remodeled through the immunomodulatory molecules, the targeting obstacle and immunosuppression bottleneck of the CAR-T therapy in solid tumor treatment are broken through, and the curative effect of immune cells based on CAR-T cells on solid tumors is remarkably improved.

The invention relates to the technical field of novel biological materials, and particularly discloses an active oxygen and lactic acid dual-driven chemotactic nano motor and a preparation method thereof. Comprising lactate oxidase and selenium nanoparticles, the selenium nano-particles are formed by ascorbic acid and sodium selenite through a chemical reduction method, and the surfaces of the selenium nano-particles are modified with polysaccharide for stabilizing the selenium nano-particles; the lactate oxidase is loaded on the selenium nano-particles to form the nano-motor. Lactic acid is specifically consumed by loading lactic acid oxidase on the surface of the selenium nano-motor, due to high selectivity, the nano-motor can perform directional chemotactic movement towards a lactic acid high expression site, and the excellent oxidation resistance of selenium nano-particles can remove active oxygen and avoid oxidative stress damage, so that the lactic acid and the active oxygen are consumed at the same time.

The application discloses a kind of nanomedicine for treating sepsis and its preparation method and purposes, belong to medical technology field.The nanomedicine is tetrahedral framework nucleic acid modified by N-acetyl proline-glycine-proline.The nanomedicine of the application can be transported to inflammatory site by following the chemotaxis of neutrophil to inflammatory site, to play the target regulation effect on sepsis, to increase the bioavailability of drug, improve the target treatment effect of drug.The nanomedicine of the application can be used not only for preparing the drug for treating sepsis, but also for preparing the drug for treating other diseases caused by neutrophil infiltration, and has wide application prospect.

A composition for preventing or treating cancer or infectious disease is disclosed. The composition contains, as an active ingredient, a pluripotent stem cell-derived immune cell expressing IL-7, CCL19, or a combination thereof. The composition provides a multifaceted and synergistic therapeutic effect derived from the complementary immune response of the patient's endogenous T cells and the injected natural killer cells, by administering only a therapeutically effective amount of immune cells other than T cells, specifically natural killer cells. The natural killer cells of the composition may also be co-administered with exogenous T cells to allow these different cell populations to act more intensively at the lesion site. The composition is based on the differentiation of pluripotent stem cells, specifically induced pluripotent stem cells (iPSCs) into immune cells, thus can be used to generate an unlimited supply of allogenic or autologous cells as needed.

This invention relates to the field of predicting microbial migration behavior in porous media, and discloses a method for predicting microbial chemotactic migration behavior based on a reactive solute transport model. The method includes the following steps: obtaining the penetration curves of microorganisms and target reactive solutes through migration experiments in porous media; constructing and calibrating a reactive solute transport model; quantifying microbial chemotactic intensity using the capillary method and establishing a functional relationship between the chemotactic rate and the solute concentration gradient; substituting the solute concentration distribution into the chemotactic function to obtain the microbial chemotactic rate, and introducing a convection-dispersion equation with a convection-like term to simulate migration behavior; finally, constructing a reactive solute transport model coupled with chemotactic parameters to predict microbial migration behavior under different initial conditions. This invention achieves deep coupling between microbial chemotactic kinetics and solute transport processes, improving prediction accuracy and providing reliable theoretical support and engineering guidance for contaminated site remediation and groundwater solute control.

The invention relates to the technical field of geotechnical engineering, in particular to an MICP inverse gradient regulation and control device and method. The device comprises a container, a channel unit, an annular sealing piece, a heat preservation cover, a driving piece and a pressure regulation and control device. Wherein the annular sealing piece sleeves the periphery of the channel unit and rotates to close the through hole through the driving piece, the pressure regulation and control device adjusts the pressure of the container, the container and the channel unit are filled with an immobilization matrix, the immobilization matrix adsorbs an immobilization solution, and the chemotactic plant is planted on the immobilization matrix in the container. The chemotactic plant releases a chemotactic signal through root exudates so as to directionally attract MICP functional microorganisms. According to the scheme provided by the invention, MICP functional microorganisms can be directionally attracted through the chemotactic signal induction module, directional migration and efficient immobilization of the microorganisms are realized in combination with through hole opening and closing control of the annular sealing element and pressure regulation of the pressure regulation and control device, and meanwhile, the stability of the internal environment is ensured by virtue of the heat preservation cover and the filter supporting layer; and the application efficiency of the MICP technology is effectively improved.

The application discloses a diagnostic marker detection system of a star-shaped glial cell IL-33 knockout EAE model, relates to the technical field of in vitro diagnosis, and comprises the following steps: constructing direction consistency constraint, four-domain prior weight and anchor point consistency scoring; selecting a minimum panel in four domains of inflammation chemotaxis, T cell subgroups, myelin axons and blood brain barrier, and generating Th17 / Th1 and MMP9 / closure protein ratio and chemotaxis combination; implementing standardized collection, platform alignment and bridge calibration, establishing intra-batch / between-batch quality control and time window limitation; performing learning with monotone constraint and two-stage calibration, and reserving animal-to-human population domain adaptation; and outputting star source index, double-threshold value stratification, longitudinal monitoring and interpretable report, so that cross-platform and cross-batch comparability, direction order preservation, traceability and clinical bridgeability are realized, and robust discrimination and reproducibility are improved.

This invention relates to a nanomedicine delivery system targeting neutrophils, its preparation method, and its application. The nanomedicine delivery system is prepared by the following method: S1, preparing liposome 1 encapsulating drug 1; S2, preparing liposome 2 encapsulating drug 2; S3, mixing and incubating the liposome 1 and liposome 2 encapsulating drug 1 and drug 2, and connecting the corresponding ligands and receptors in liposome 1 and liposome 2 to obtain the nanomedicine delivery system; the ligands and receptors are biotin and streptavidin, respectively. This invention successfully connects two drug liposomes with different functions through a ligand and receptor system to obtain the nanomedicine delivery system, which can enhance the chemotaxis of neutrophils to the site of infection and improve the antibacterial properties of NETs, ​​exhibiting very good anti-infective effects.

A method for preparing and applying immobilized enzyme nanogels includes oxidized sodium alginate and enzymes are dissolved separately in deionized water, mixed uniformly, and reacted to obtain immobilized enzyme nanogels; the aldehyde groups in oxidized sodium alginate react with the amino groups in the enzymes, forming imine bonds to immobilize the enzymes; forming nano-scale cross-linked polymer-based particles with a three-dimensional network structure, resulting in the immobilized enzyme nanogels; the surface of immobilized collagenase nanogels is modified with CXCR4 antagonist peptides, which can specifically block CXCR4 on the surface of T cells; the Schiff base bonds can selectively break in the acidic microenvironment of tumors, leading to collagen degradation and reduced extracellular matrix density; the modification enhances the chemotaxis and infiltration of T cells into pancreatic cancer tissues and can inhibit the metastasis of pancreatic cancer.