40 results about "Chemotaxis" patented technology

The application discloses a sludge biological drying multi-target proteinase regulation method based on bacterial chemotaxis, relates to the technical field of sludge resource disposal, and comprises the following steps: S1, constructing a microfluidic detection system based on the chemotaxis of thermophilic bacteria, and monitoring and quantifying the metabolic preference degree of the thermophilic bacteria to different amino acids in a sludge biological drying process; S2, selecting and proportioning proteinases with a targeted degradation function according to the metabolic preference degree of the thermophilic bacteria; and S3, decomposing refractory proteins into specific amino acids preferred by the thermophilic bacteria through multi-target regulation of the proteinases. The application optimizes the metabolic environment of the thermophilic bacteria to improve the utilization rate of protein organic matter and accelerate the sludge biological drying process.

The invention relates to the technical field of microbial preparation optimization and intelligent algorithm crossing, and particularly discloses a foraging algorithm for coupling dominant functional bacteria of targeted biological bacteria and host original flora, and the foraging algorithm simulates a coupled symbiotic system of the targeted biological bacteria and the host original flora into a flora society. Each to-be-optimized single-function bacterial strain is regarded as a'bacterial 'individual in the algorithm, the comprehensive effect of a flora on a specific target is used as a fitness function, global optimization is carried out through an improved chaos optimization bacterial foraging algorithm, and through the steps of flora proportion initialization, chemotaxis operation, breeding operation, migration operation and the like, the optimal bacterial foraging algorithm is obtained. The method provided by the invention solves the problems of complex work, long period and difficulty in obtaining a globally optimal solution in the traditional'trial-and-error method 'for preparing the composite flora, and provides core algorithm support for quickly, accurately and intelligently developing an efficient composite microbial preparation.

The invention belongs to the technical field of biology, and particularly relates to application of a herba artemisiae scopariae aqueous extract in enhancing liver chemotaxis of adipose-derived mesenchymal stem cells. In order to provide a way for enhancing the liver chemotaxis of the adipose-derived stem cells, the capillary artemisia aqueous extract is used for improving the expression level of chemotactic factors of the adipose-derived stem cells in vitro and intervening the proliferation, migration and chemotaxis capabilities of the adipose-derived stem cells to liver cells, the concentration of the capillary artemisia aqueous extract is 0.5 mg / mL to 1 mg / mL, the chemotaxis time is 24 h to 48 h, and the capillary artemisia aqueous extract is used for enhancing the liver chemotaxis of the adipose-derived stem cells. And the chemotactic factors are CCR1 and CXCR4.

The invention discloses an application of a CD177 targeted membrane modified liposome in preparation of a medicine for preventing and / or treating psoriasis. The structure of the CD177 targeting membrane modified liposome comprises a PADI4 inhibitor loaded liposome, a targeting recognition layer formed by coupling CD177 targeting peptide on the surface of the liposome, and a bionic functional layer formed by coating a natural neutrophile granulocyte membrane on the outermost layer. Based on the core pathological mechanism of 'CD177 + neutrophil-NETs' of psoriasis, the inflammation chemotaxis / cell factor neutralization function of a neutrophil membrane, the precise targeting function of CD177 peptide and the NETs inhibition function of a PADI4 inhibitor are creatively and organically integrated, and the constructed GNLC achieves the synergistic treatment effect of 'targeting enrichment-mechanism blocking-microenvironment remodeling'; meanwhile, excellent stability and safety are achieved, and a new precise treatment strategy far better than that in the prior art is provided for psoriasis.

The invention discloses a macrophage-platelet targeting delivery system carrying genes and mitochondria as well as a preparation method and application of the macrophage-platelet targeting delivery system. The platelet loaded with the PPAR gamma gene nano-composite is covalently linked to the surface of macrophage through a click chemical reaction, and a novel cell composite carrier is constructed. According to the system, the natural chemotaxis of macrophages to an injured part is fully utilized to realize precise targeting, and PPAR gamma genes and functional mitochondria are synchronously released through platelet activation in an injured microenvironment, so that the energy metabolism and lipid homeostasis of the macrophages are cooperatively regulated and controlled, the cellular burial function of the macrophages is effectively recovered, and tissue repair and regeneration are promoted. Experimental results show that the delivery system is reliable in preparation method and high in targeting efficiency, axon regeneration, myelin sheath repair and motor function recovery can be remarkably promoted in a spinal cord injury model, and a brand-new synergistic treatment strategy is provided for tissue injury repair.

This invention discloses a microfluidic chip for three-stage non-destructive sperm screening and its usage method. The chip includes a substrate, a sample inlet channel layer, a sample chamber layer, a recovery chamber layer, and a screening filter membrane. The sample inlet channel layer has an inlet channel and an overflow channel. The sample chamber layer has an annular sample chamber, an inlet orifice, and an overflow orifice. The recovery chamber layer is divided into an outer recovery chamber and an inner recovery chamber by a dike. An inducing agent patch is located in the center of the inner recovery chamber. The screening filter membrane is sandwiched between the sample chamber layer and the recovery chamber layer. In use, after injecting a sperm sample, initial motility screening is achieved through the screening filter membrane. Secondary chemotactic screening is completed through an inducing agent concentration gradient. Tertiary adaptive screening for swimming direction is completed using the chemotactic channel. Finally, high-quality sperm are obtained from the inner recovery chamber. This invention achieves multi-parameter graded screening, effectively avoiding sperm damage, improving sperm recovery quality and screening efficiency, and is applicable to various assisted reproductive technologies, helping to improve conception success rates.

The application discloses a di(2-ethylhexyl) phthalate degrading bacterium, a bacterial composition and application thereof. The degrading bacterium is named Sphingomonas parapaucimobilis GHQ28 and Stenotrophomonas maltophilia GHQ31. Research finds that GHQ28 and GHQ31 have chemotaxis to DEHP and downstream metabolites thereof, and there is a synergistic interaction between the two bacteria; the growth and DEHP degradation speed of the two strains under co-culture conditions are faster than that under single culture conditions. The bacterial composition composed of the two strains has a highest degradation rate of 99.2±0.59% to 400mg / L DEHP within 72h, compared with single culture, the total biomass is increased by 1.4-50.3 times, and the DEHP degradation rate is increased by 1.07-58.82 times. The application provides a new technical means for remediation of DEHP contaminated soil or water.

The invention provides a preparation process of a soothing and repairing type mask, and belongs to the technical field of cosmetics, an intelligent response type cell bionic delivery system is adopted, macrophage membrane vesicles serve as a carrier, a composite repairing kernel, epicatechin gallate, a neuropeptide P substance antagonist and idebenone are wrapped, and by means of the inflammation chemotaxis of a macrophage membrane, the soothing and repairing type mask is prepared. According to the present invention, the active component can actively target the inflammation region, the phase change release core is provided in the weakly acidic microenvironment with the pH value of 5.0-6.0, the targeting property and the permeation efficiency of the active component can be improved, the bottleneck of the traditional passive diffusion can be broken through, and compared with the conventional liposome or nanoparticle, the active component can be accurately delivered to the inflammation core region, the permeation rate can be improved by several times, and the carrier can directly reach the problem core region; according to the method, the utilization rate of effective components is greatly increased, a micro-ecology-immunity-nerve-physical barrier multi-dimensional and systematic synergistic repair network is constructed, full-path fundamental repair from immediate relieving to root strengthening is achieved, and immediate relieving and long-term barrier repair effects are provided.

The invention discloses an application of a Pik3cb inhibitor in treatment of Sjogren's disease. Experiments prove that the Pik3cb inhibitor TGX-221 can reduce the number, activation and chemotaxis of NOD mouse B cells of a Sjogren's disease model mouse, plasma cell differentiation and antibody secretion, improve the immune microenvironment of the submaxillary gland of the Sjogren's disease model mouse, reduce the infiltration degree of submaxillary gland lymphocytes and promote the salivary secretion function; and after the Pik3cb is over-expressed, the symptom and pathology of the Sjogren's disease are aggravated. Therefore, the Pik3cb inhibitor can be used for treating the Sjogren's disease. The invention provides a new thought for research and development of medicines for treating the Sjogren's disease, and has important application value.

The invention provides a method for enhancing the tumor homing ability of TIL cells, and belongs to the technical field of biological medicine. The invention provides a method for effectively enhancing the homing ability of TIL cells, so that the chemotactic and enrichment potential of engineered TIL cell products to tumor sites is remarkably improved. The TIL cell preparation prepared on the basis of the method has higher tumor targeting performance, and a new solution is provided for overcoming the bottleneck that effector cells'reach 'tumor parts in an existing cell therapy. The effectiveness of single cell therapy is expected to be directly improved, and better cell products can be provided for synergistic combination with other therapies such as radiotherapy and chemotherapy, so that wider and more effective treatment choices are brought to patients with solid tumors.

This invention proposes a method for preparing a neutrophil membrane-like biomimetic nanoplatform, characterized by the following steps: Step 1: Synthesizing a 4-octyl itaconic acid-retinol prodrug (OR); Step 2: Preparing ORNPs; Step 3: Extracting dHL-60 cell membranes; Step 4: Assembling M@ORNPs to obtain core-shell structured M@ORNPs nanoparticles with a hydration kinetic diameter of approximately 108.2 nm, which constitute the biomimetic nanoplatform. The biomimetic nanoplatform possesses active chemotaxis and immune escape properties, enabling precise delivery of 4-octyl itaconic acid-retinol in sepsis-associated acute lung injury.

The application provides a kind of lipopeptide compound PW-1 and its preparation method and application in preventing and controlling bacterial wilt, belong to biological pesticide technical field;The PW-1 is prepared by fermentation, solvent extraction, acidification and reverse phase chromatography from Paenibacillus polymyxa (P. Paenibacillus polymyxa ) strain DW-7A;The core of the compound is that it plays a role through "anti-toxicity" mechanism, at the concentration without inhibiting the growth of Pseudomonas solanacearum (P. Ralstonia solanacearum ), can specifically interfere with the biosynthesis of pathogenic bacteria flagellum, cause its motility, chemotaxis and early infection ability to decrease significantly;Pot experiment proves that the relative control efficiency of PW-1 to tobacco bacterial wilt reaches 62.2%, and has no effect on the growth of soil bacillus at the concentration of up to 1000 μg / mL, shows efficient, targeted and environment compatible characteristics;The application provides a new candidate compound and key technical support for the development of green pesticide targeting flagellum.

According to the engineered carrier bacterium mediated T cell tumor targeted collaborative treatment system, through delivery of modified carrier bacteria, immunomodulatory molecules are loaded to the carrier bacteria through covalent bonds and / or non-covalent bonds by utilizing natural chemotaxis of the carrier bacteria to a tumor immune microenvironment, and immunomodulatory molecule synergistic carrier bacteria are constructed; the engineering bacteria and CAR-T cells are coupled through covalent bonds and / or non-covalent bonds to form a CAR-T-bacteria chimera. The chimera realizes CAR-T cell tumor enrichment by virtue of the tumor targeting property of carrier bacteria; meanwhile, the immune microenvironment is remodeled through the immunomodulatory molecules, the targeting obstacle and immunosuppression bottleneck of the CAR-T therapy in solid tumor treatment are broken through, and the curative effect of immune cells based on CAR-T cells on solid tumors is remarkably improved.

The invention relates to the technical field of novel biological materials, and particularly discloses an active oxygen and lactic acid dual-driven chemotactic nano motor and a preparation method thereof. Comprising lactate oxidase and selenium nanoparticles, the selenium nano-particles are formed by ascorbic acid and sodium selenite through a chemical reduction method, and the surfaces of the selenium nano-particles are modified with polysaccharide for stabilizing the selenium nano-particles; the lactate oxidase is loaded on the selenium nano-particles to form the nano-motor. Lactic acid is specifically consumed by loading lactic acid oxidase on the surface of the selenium nano-motor, due to high selectivity, the nano-motor can perform directional chemotactic movement towards a lactic acid high expression site, and the excellent oxidation resistance of selenium nano-particles can remove active oxygen and avoid oxidative stress damage, so that the lactic acid and the active oxygen are consumed at the same time.

This invention relates to the field of predicting microbial migration behavior in porous media, and discloses a method for predicting microbial chemotactic migration behavior based on a reactive solute transport model. The method includes the following steps: obtaining the penetration curves of microorganisms and target reactive solutes through migration experiments in porous media; constructing and calibrating a reactive solute transport model; quantifying microbial chemotactic intensity using the capillary method and establishing a functional relationship between the chemotactic rate and the solute concentration gradient; substituting the solute concentration distribution into the chemotactic function to obtain the microbial chemotactic rate, and introducing a convection-dispersion equation with a convection-like term to simulate migration behavior; finally, constructing a reactive solute transport model coupled with chemotactic parameters to predict microbial migration behavior under different initial conditions. This invention achieves deep coupling between microbial chemotactic kinetics and solute transport processes, improving prediction accuracy and providing reliable theoretical support and engineering guidance for contaminated site remediation and groundwater solute control.

The invention relates to the technical field of geotechnical engineering, in particular to an MICP inverse gradient regulation and control device and method. The device comprises a container, a channel unit, an annular sealing piece, a heat preservation cover, a driving piece and a pressure regulation and control device. Wherein the annular sealing piece sleeves the periphery of the channel unit and rotates to close the through hole through the driving piece, the pressure regulation and control device adjusts the pressure of the container, the container and the channel unit are filled with an immobilization matrix, the immobilization matrix adsorbs an immobilization solution, and the chemotactic plant is planted on the immobilization matrix in the container. The chemotactic plant releases a chemotactic signal through root exudates so as to directionally attract MICP functional microorganisms. According to the scheme provided by the invention, MICP functional microorganisms can be directionally attracted through the chemotactic signal induction module, directional migration and efficient immobilization of the microorganisms are realized in combination with through hole opening and closing control of the annular sealing element and pressure regulation of the pressure regulation and control device, and meanwhile, the stability of the internal environment is ensured by virtue of the heat preservation cover and the filter supporting layer; and the application efficiency of the MICP technology is effectively improved.

The application discloses a diagnostic marker detection system of a star-shaped glial cell IL-33 knockout EAE model, relates to the technical field of in vitro diagnosis, and comprises the following steps: constructing direction consistency constraint, four-domain prior weight and anchor point consistency scoring; selecting a minimum panel in four domains of inflammation chemotaxis, T cell subgroups, myelin axons and blood brain barrier, and generating Th17 / Th1 and MMP9 / closure protein ratio and chemotaxis combination; implementing standardized collection, platform alignment and bridge calibration, establishing intra-batch / between-batch quality control and time window limitation; performing learning with monotone constraint and two-stage calibration, and reserving animal-to-human population domain adaptation; and outputting star source index, double-threshold value stratification, longitudinal monitoring and interpretable report, so that cross-platform and cross-batch comparability, direction order preservation, traceability and clinical bridgeability are realized, and robust discrimination and reproducibility are improved.

The application discloses a kind of tumor angiogenesis simulation methods, including the blood vessel network topology including environment pixel point and blood vessel pixel point generated based on the medical image of patient;Based on the average path length and step length adjustment factor, chemotaxis weight, bifurcation number range and bifurcation probability of related data such as blood vessel tip of blood vessel network topology are constantly updated;And the bifurcation number of each iteration blood vessel tip and the branch length of each branch after bifurcation are calculated based on the update result of each iteration, and the update of blood vessel network topology is completed based on the bifurcation number and the branch length of each branch after bifurcation calculated, constantly circulate until reaching simulation condition.The application quantifies the transport efficiency of blood vessel network by global function index average path length, realizes the evaluation whether blood vessel network presents efficient local characteristics by clustering coefficient and average path length, guarantees that the blood vessel network obtained by simulation is same with real network.

The invention belongs to the technical field of agricultural biological protection, and discloses a lysozyme-siderophore composite preparation based on root signal decoy and application of the lysozyme-siderophore composite preparation in prevention and control of banana Panama disease, in the composite preparation, a TR4 chemotactic receptor antagonist simulates banana root exudate gamma-aminobutyric acid, magnetic nanoparticles Fe3O4 (at) SiO2 guide the agent to enrich the root system and release Fe < + > to PDCA, so that the banana root exudate gamma-aminobutyric acid and the magnetic nanoparticles Fe3O4 (at) SiO2 guide the agent to enrich the root system; fe < + > activates the magnetic nanoparticles to produce heat, lysozyme is locally heated to 42 DEG C to release the shell to release the shell, lysozyme and quenching enzyme in a targeted manner, the TR4 chemotaxis is reversely utilized to realize precise trapping and killing, pathogenic virulence factors are converted into medicament activation signals, diseased plant conduit regeneration is realized through plant peptides, and the method is suitable for prevention and control of banana panama disease of musaceae crops such as bananas and musa basjoo.

This invention discloses the use of a bacterial membrane dCACHE-type chemokine receptor protein family as a target in the preparation or screening of drugs against multidrug-resistant bacteria, belonging to the field of biomedical technology. This invention reveals and verifies for the first time the core regulatory mechanism of the dCACHE-type chemokine receptor protein family in the bacterial infection process: these proteins are key membrane surface receptors mediating bacterial chemotaxis towards macrophages; their loss of function can directly block bacterial chemotactic movement and significantly reduce pathogenicity. This invention, by targeting the conserved ligand binding interface, successfully inhibited the infectivity of drug-resistant pathogens such as *Yersinia pseudotuberculosis*, carbapenem-resistant *Acinetobacter baumannii*, and carbapenem-resistant *Escherichia coli*. The novel targeting strategy provided by this invention not only effectively overcomes the clinical bottleneck of "no available drugs" for carbapenem-resistant multidrug-resistant bacteria but also constructs a broad-spectrum protective barrier covering the entire homologous protein family, possessing extremely high clinical translational value and commercial development potential.

The present invention relates to a pharmaceutical composition and the use thereof, in particular to the use of a combination of a dual-target CAR-T targeting TNFRSF10B / ASGR1 and a formula for strengthening vital qi, removing toxins and removing food retention in the preparation of a composition for treating liver cancer. A pharmaceutical composition comprising a TNFRSF10B / ASGR1 dual-target chimeric antigen receptor T cell and a formula for strengthening vital qi, removing toxins and removing food retention, wherein the TNFRSF10B / ASGR1 dual-target chimeric antigen receptor is expressed on the membrane surface of the dual-target chimeric antigen receptor T cell. It is unexpectedly found that the combined use of the formula for strengthening vital qi, removing toxins and removing food retention with CAR-T can effectively inhibit the activation of the STAT3 pathway, promote the chemotaxis of a CAR-T cell as an effector T cell, improve the infiltration and cytotoxicity, inhibit the depletion of CD8+T cells, and effectively improve the anti-liver cancer capability of the CAR-T cell.

The invention discloses an adjustable and controllable variable model for exploring phototaxis and chemotaxis of insects, and relates to the technical field of insect research, the adjustable and controllable variable model comprises a main box body, the main box body is made of a light-proof material, and the interior of the main box body is divided into a phototaxis experiment area, a central release area and a chemotaxis experiment area which are adjacent in sequence through two parallel partition plates; one-way selection channels are formed in the partition plates on the two sides, an adjustable light source module is arranged in the phototaxis experiment area, a smell diffusion module is arranged in the chemotaxis experiment area, and the phototaxis experiment area can serve as a demonstration device during teaching and can also serve as a magic box for experiment exploration of interest groups. The phototaxis and chemotaxis of insects are independently explored according to the principle of a single variable, and the effect of light and sex pheromones on insect taxis together can be researched when light and female insects exist at the same time.

The invention relates to application of a CXCL14 recombinant protein in preparation of a medicine for preventing or treating radiation enteritis, and belongs to the technical field of biomedicine. Excessive inflammation is inhibited by regulating chemotaxis and pro-inflammatory polarization of key immune cells such as macrophages. The CXCL14 recombinant protein directly acts on intestinal epithelial cells and crypt stem cells to promote intestinal villus repair and crypt regeneration, so that the intestinal barrier is structurally recovered. The specific value of intestinal villus length / crypt depth is remarkably increased, and it is proved that the CXCL14 recombinant protein can achieve functional healing instead of symptom relieving.

The present invention relates to a citric acid-based particulate injection material, a preparation method therefor, and the use thereof. Citric acid and 1,8-octanediol are melted and heated to form a POC prepolymer; the POC prepolymer is dissolved by means of an organic solvent to obtain a dissolved solution, and the dissolved solution is slowly poured into ultrapure water multiple times for purification to obtain a purified POC degradable prepolymer; the POC degradable prepolymer is dissolved by means of a solvent, inorganic salt particles are added, and a crosslinking reaction is carried out to obtain a POC injectable particulate material. A drug, an active molecule, and metal ions are loaded onto the POC by means of physical compounding or chemical crosslinking to obtain a citric acid-based particulate injection material. As a novel aesthetic medicine raw material and a related particulate injection filler thereof, the citric acid-based biomaterial provided in the present invention can effectively promote the regeneration, proliferation, migration, and chemotaxis of skin cells, promote the production of collagen, and achieve an aesthetic medicine effect. The present invention can also activate the body's immune response to reduce inflammation, and a product after degradation is citric acid, which can be metabolized by the body with no obvious immunogenicity and toxic side effects.

The invention discloses an indoline derivative and application thereof. According to the invention, the regulation effect of the derivative on the chemotaxis and secretion functions of ILC3 is evaluated; it is found that the derivative has a promoting effect on chemotaxis of ILC3 to an inflammatory site and up-regulation of IL-22 expression, has very remarkable effects on mouse ulcerative colitis (UC) induced by DSS, mouse Crohn disease (CD) induced by TNBS, acute lung injury induced by lipopolysaccharide (LPS), mouse psoriasis induced by imiquimod and other diseases in vivo, and is superior to existing clinical treatment drugs. Good development and application prospects are realized.

Controlled release of VIP from PLGA microparticles was accomplished and varied through use of different polymer molecular sizes, addition of solutes to the inner aqueous phase, and use of our computer model. Released VIP from microparticles appeared to be bioactive and caused DCs to produce more CCL22 than DCs treated with blank particles at 7 and 24 hours. Additionally, DCs treated with VIP microparticle releasates recruited higher percentages of FoxP3+ T-cells in in vitro chemotaxis studies. Testing in a mouse model in vivo indicated that VIP microparticles have significant therapeutic potential to treat periodontal disease by reducing the bone loss in infected mice relative to the blank group.

The invention aims to provide an acid response type rheumatoid arthritis targeted therapy nano preparation as well as a synthesis method and application thereof, and belongs to the technical field of preparation of nano biomedical materials. The preparation method comprises the following steps: synthesizing Zn / SiO nano-particles with a dandelion-shaped structure through an oil-water two-phase method, loading methotrexate by utilizing the large-aperture characteristic of the particles, and coating an M2 macrophage membrane on the outer layer to prepare the methotrexate-loaded Zn / SiO nano-particles. The nano preparation has good biocompatibility, stability and capability of targeting rheumatoid arthritis parts by virtue of inflammation chemotaxis and homologous targeting effects of M2 macrophage membranes. In an acidic microenvironment, the nanoparticles can respond to release zinc ions and drugs, so that safe and effective treatment of rheumatoid arthritis is realized.

The application discloses a high-performance neutrophil screening method and system, and the method comprises the following steps: dividing neutrophils into three groups, respectively detecting the resting and post-stimulation expression data of CD10, P2X1, CD66b and CD11b; calculating the CD66b and CD11b function indexes, maturity regulation parameters and chemotaxis function parameters based on the detection data; performing weighted calculation based on the four parameters to obtain the neutrophil reaction intensity, wherein the weight of each parameter is determined based on the relative contribution degree of each parameter in different functional stages of neutrophil immune response; and screening high-performance neutrophils based on the comparison result of the neutrophil reaction intensity and a reference threshold value. The method realizes comprehensive quantitative evaluation of the complete function chain of neutrophils, and provides a reliable basis for immunological research and cell screening.

The present application relates to the field of optical fiber sensing technology, and particularly to a method and system for building a seismic monitoring platform based on an optical fiber seismometer, a three-dimensional geological model of a monitoring area is constructed based on multi-source geological data of the monitoring area; a grid element in the three-dimensional geological model is taken as a virtual particle, and the position, motion direction and chemotaxis parameter of each virtual particle are initialized; the intensity of a seismic precursor signal of the grid element is determined as the concentration of a chemical attractant of the virtual particle; a phototaxis mechanism is introduced to simulate the group motion behavior of the virtual particle under the concentration gradient of the chemical attractant, and the final distribution density map of the virtual particle is obtained; according to the final distribution density map of the virtual particle, an optimal layout map of the optical fiber seismometer in the monitoring area is determined, and the seismic monitoring platform is built according to the optimal layout map, the present application can accurately identify a potential seismic source area, dynamically adapt to changes in the geological environment, and improve the coverage accuracy and response capability of the seismic monitoring platform.