98 results about "Chemotaxis" patented technology

Burkholderia, and a composition, use and usage method thereof. Provided are the Burkholderia, and a composition comprising the strain and / or a metabolite, culture, fermentation broth and / or extract thereof. The Burkholderia or the composition thereof can generate a siderophore, induce plants or seeds thereof to generate systemic resistance to plant pathogens, and reduce the chemotaxis of plant pathogens to plants, so that prevention and treatment of plant diseases and insect pests are achieved. In addition, plant root system development can be promoted, a nitrogen fixation effect is provided, a phosphate solubilization effect is provided, indoleacetic acid is generated, and weeds are removed, thereby promoting plant growth.

The invention discloses a lipidosome drug delivery system for targeted therapy of brain glioma and application thereof. The lipidosome drug delivery system is a lipidosome mixture prepared from a basic component, a functional component and an external water-phase buffer solution. After intravenous injection, the liposome is efficiently combined with complement protein C3 and immune globulin M in plasma to synergistically activate a complement system, actively attracts and activates neutrophils to uptake, and crosses a blood brain barrier in a mode of'building a defecation vehicle 'by utilizing the inflammatory taxis of the neutrophils. Neutrophile granulocytes are stimulated by inflammatory factors in a microenvironment after reaching a tumor brain area to form extracellular trap release lipidosome. The lipidosome realizes specific targeting through folate receptors over-expressed on the surfaces of glioma cells. The liposome shows excellent cross-blood-brain-barrier drug delivery efficiency and brain glioma curative effect in vivo, can be effectively combined with surgery, radiotherapy and the like, and provides a solution for the main problems of radiotherapy resistance, chemotherapy drug resistance and the like in clinic.

Modified bacterial strain of the Pseudomonas sp. genus, the strain of Pseudomonas stutzeri deposited under Deposit Accession No. RGM3505 and a modified bacterial strain of the Bacillus sp. genus, a strain of Bacillus velezensis deposited under Accession No. RGM3504. The strains may be combined at a Pseudomonas stutzeri to Bacillus velezensis ratio from 1 :10 to 10:1 CFU / mL. The strains were modified to have greater chemotactic attraction towards plant roots and to improve the beneficial features of crops, for example, of legumes.

The application discloses a sludge biological drying multi-target proteinase regulation method based on bacterial chemotaxis, relates to the technical field of sludge resource disposal, and comprises the following steps: S1, constructing a microfluidic detection system based on the chemotaxis of thermophilic bacteria, and monitoring and quantifying the metabolic preference degree of the thermophilic bacteria to different amino acids in a sludge biological drying process; S2, selecting and proportioning proteinases with a targeted degradation function according to the metabolic preference degree of the thermophilic bacteria; and S3, decomposing refractory proteins into specific amino acids preferred by the thermophilic bacteria through multi-target regulation of the proteinases. The application optimizes the metabolic environment of the thermophilic bacteria to improve the utilization rate of protein organic matter and accelerate the sludge biological drying process.

The invention belongs to the field of plant protection, and discloses a method for inducing bemisia tabaci by a persicaria orientalis volatile matter and a related application of the persicaria orientalis volatile matter to the bemisia tabaci. A Y-type olfactometer behavior experiment shows that the bemisia tabaci has a remarkable chemotactic reaction on the red-knees herb, and the preference of the bemisia tabaci is obviously higher than that of a common host tomato. Furthermore, solid phase microextraction (SPME) is combined with gas chromatography-mass spectrometry (GC-MS) to analyze the smell components of the polygonum hydropiper, and various volatile substances possibly participating in chemotaxis are screened out. The method can be used for developing a plant source smell attractant and improving the pest monitoring and green prevention and control efficiency.

The invention relates to a collaboration and grouping method for swarm robots inspired by bacterial tropism, which comprises the following steps of: establishing a fitness function of a robot according to position information of neighbors in a local sensing range of an individual robot on the basis of a bacterial food source sensing mechanism; based on the fitness function and the bacterial tropism mechanism, determining the basic behavior state of the robot, and obtaining a motion switching result about whether behavior state switching occurs or not; correcting the motion state of the robot and determining the rotation angle of the robot based on the switching result; wherein the rotation angle is used for controlling the swarm robots to keep the same kind of subgroups agglomerated, keep away from different kinds of groups and search for other same kind of individuals, gathering in a certain area of the environment, and grouping the swarm robots. According to the method, the robot behavior state is determined by analyzing the bacterial tropism mechanism, the behavior state switching mechanism is constructed, the rotation angle is corrected, the swarm robot grouping effect is achieved, swarm robot distributed cooperative grouping is achieved, and the grouping efficiency is improved.

The invention relates to the technical field of microbial preparation optimization and intelligent algorithm crossing, and particularly discloses a foraging algorithm for coupling dominant functional bacteria of targeted biological bacteria and host original flora, and the foraging algorithm simulates a coupled symbiotic system of the targeted biological bacteria and the host original flora into a flora society. Each to-be-optimized single-function bacterial strain is regarded as a'bacterial 'individual in the algorithm, the comprehensive effect of a flora on a specific target is used as a fitness function, global optimization is carried out through an improved chaos optimization bacterial foraging algorithm, and through the steps of flora proportion initialization, chemotaxis operation, breeding operation, migration operation and the like, the optimal bacterial foraging algorithm is obtained. The method provided by the invention solves the problems of complex work, long period and difficulty in obtaining a globally optimal solution in the traditional'trial-and-error method 'for preparing the composite flora, and provides core algorithm support for quickly, accurately and intelligently developing an efficient composite microbial preparation.

Provided are a bionic drug-loaded nanoparticle with expressed CXCR4, a method for preparing same, and use thereof. The method comprises: mixing a lactic acid-glycolic acid copolymer and an immunosuppressant in a certain ratio, and preparing the mixture into a drug-loaded nanoparticle using a microfluidic technology; preparing an MSC cell stably expressing membrane protein CXCR4 and lysing same to extract the cell membrane, so as to give an engineered stem cell membrane; and fusing the engineered stem cell membrane with the drug-loaded nanoparticle to give a bionic drug-loaded nanoparticle with expressed CXCR4. The bionic drug-loaded nanoparticle with expressed CXCR4 is a bionic cell membrane drug-loaded nanoparticle with chemotaxis functionality. By means of genetic engineering, the engineered stem cell membrane is modified to encapsulate a therapeutic agent, and directionally migrates to the lesions in the body and targets specific cells, thereby improving the drug distribution in vivo and reducing adverse effects of immunosuppressants.

The invention belongs to the technical field of biology, and particularly relates to application of a herba artemisiae scopariae aqueous extract in enhancing liver chemotaxis of adipose-derived mesenchymal stem cells. In order to provide a way for enhancing the liver chemotaxis of the adipose-derived stem cells, the capillary artemisia aqueous extract is used for improving the expression level of chemotactic factors of the adipose-derived stem cells in vitro and intervening the proliferation, migration and chemotaxis capabilities of the adipose-derived stem cells to liver cells, the concentration of the capillary artemisia aqueous extract is 0.5 mg / mL to 1 mg / mL, the chemotaxis time is 24 h to 48 h, and the capillary artemisia aqueous extract is used for enhancing the liver chemotaxis of the adipose-derived stem cells. And the chemotactic factors are CCR1 and CXCR4.

The invention provides application of N-methyl-L-proline in regulation and control of chemotaxis of bacillus amyloliquefaciens, and belongs to the technical field of plant disease prevention and control. The bacillus amyloliquefaciens provided by the invention has a remarkable chemotactic effect on the N-methyl-L-proline, so that the enrichment and colonization capabilities of the bacillus amyloliquefaciens can be remarkably improved by the N-methyl-L-proline. The invention further provides a method for promoting proliferation and / or colonization of the bacillus amyloliquefaciens, the colonization and growth promoting effects of the bacillus amyloliquefaciens are improved, and the colonization amount of the bacillus amyloliquefaciens can be remarkably increased.

The invention discloses an application of a CD177 targeted membrane modified liposome in preparation of a medicine for preventing and / or treating psoriasis. The structure of the CD177 targeting membrane modified liposome comprises a PADI4 inhibitor loaded liposome, a targeting recognition layer formed by coupling CD177 targeting peptide on the surface of the liposome, and a bionic functional layer formed by coating a natural neutrophile granulocyte membrane on the outermost layer. Based on the core pathological mechanism of 'CD177 + neutrophil-NETs' of psoriasis, the inflammation chemotaxis / cell factor neutralization function of a neutrophil membrane, the precise targeting function of CD177 peptide and the NETs inhibition function of a PADI4 inhibitor are creatively and organically integrated, and the constructed GNLC achieves the synergistic treatment effect of 'targeting enrichment-mechanism blocking-microenvironment remodeling'; meanwhile, excellent stability and safety are achieved, and a new precise treatment strategy far better than that in the prior art is provided for psoriasis.

The invention discloses an indoline derivative and application thereof. According to the invention, the regulation effect of the derivative on the chemotaxis and secretion functions of ILC3 is evaluated; it is found that the derivative has a promoting effect on chemotaxis of ILC3 to an inflammatory site and up-regulation of IL-22 expression, has very remarkable effects on mouse ulcerative colitis (UC) induced by DSS, mouse Crohn disease (CD) induced by TNBS, acute lung injury induced by lipopolysaccharide (LPS), mouse psoriasis induced by imiquimod and other diseases in vivo, and is superior to existing clinical treatment drugs. Good development and application prospects are realized.

The invention discloses a macrophage-platelet targeting delivery system carrying genes and mitochondria as well as a preparation method and application of the macrophage-platelet targeting delivery system. The platelet loaded with the PPAR gamma gene nano-composite is covalently linked to the surface of macrophage through a click chemical reaction, and a novel cell composite carrier is constructed. According to the system, the natural chemotaxis of macrophages to an injured part is fully utilized to realize precise targeting, and PPAR gamma genes and functional mitochondria are synchronously released through platelet activation in an injured microenvironment, so that the energy metabolism and lipid homeostasis of the macrophages are cooperatively regulated and controlled, the cellular burial function of the macrophages is effectively recovered, and tissue repair and regeneration are promoted. Experimental results show that the delivery system is reliable in preparation method and high in targeting efficiency, axon regeneration, myelin sheath repair and motor function recovery can be remarkably promoted in a spinal cord injury model, and a brand-new synergistic treatment strategy is provided for tissue injury repair.

The application discloses a mobile edge computing task offloading method based on a bacterial foraging algorithm, and steps of the method comprise the following steps: S1, constructing a many-to-many edge computing offloading model; S2, establishing a time delay model and an energy consumption model of an edge server and a mobile device; S3, taking the longest time delay on the edge server and the mobile device as the total time delay of the system, taking the total energy consumption as the system energy consumption, obtaining a system optimization total target by weighting, obtaining the system optimal target by using an improved bacterial foraging algorithm, and obtaining an optimal offloading result; S4, modifying a three-layer loop nested structure of the bacterial foraging algorithm, changing the bacterial foraging algorithm into a single-layer loop structure in parallel with chemotaxis and migration, and using the improved bacterial foraging algorithm to continuously iteratively update the bacterial position and obtain the best offloading strategy and the system optimization target. The method can ensure that all the mobile devices in the system can reasonably offload the computing task to the edge server or process the computing task on the mobile device, so that the system time delay and the energy consumption are reduced.

An assay system permits study of cell migration through tissue-relevant extracellular matrices under a chemokine gradient within a modular assay platform. Activated CD3+ T-cells were detected, quantified, and studied as they migrated towards chemokines (CXCL10 and CXCL12) and chemokine mimetics (CXCR3 agonist) using the assay system. Discovery of drugs, and the study of microenvironments, stimuli, and responding cells are facilitated by the assay system.

The invention relates to a targeted temperature-sensitive liposome loaded with hydrophilic and hydrophobic drugs, and a preparation method and application thereof, and belongs to the technical field of drug delivery. The invention provides a temperature-sensitive liposome simultaneously loading hydrophilic and hydrophobic drugs. The temperature-sensitive liposome comprises a liposome as a carrier, a hydrophobic anti-cancer drug and a light-sensitive material are loaded between the bimolecular layers of the liposome; a hydrophilic anti-cancer drug doxorubicin hydrochloride is wrapped in the carrier. The invention further provides a delivery system wrapped by an antibody, and the antibody is further modified on the surface of the temperature-sensitive liposome. The antibody-coated delivery system can actively target the activated neutrophil, and the chemotaxis of the tumor microenvironment to the neutrophil is utilized to realize the efficient enrichment of the drug at the tumor site, thereby achieving the targeted therapy and controllable release of the tumor.

The invention discloses an intelligent electric control spraying device and a nanofiber bone repair membrane preparation method. The device comprises a controllable nozzle, a nozzle solution box, a movable guide rail, a speed change gear box, a negative pressure aspirator and a material containing box. Growth factors in gradient concentration distribution are loaded on the repair material by controlling the spraying speed, the advancing speed and the advancing direction, in-situ immersion of the loaded growth factors in the repair material is achieved through negative pressure suction, and through the bidirectional gradient VEGF functionalized absorbable nanofiber bone repair membrane, the concentration of VEGF loaded in the central area is high, and the absorbable nanofiber bone repair membrane has good biocompatibility. The concentration of VEGF loaded to the two ends is gradually reduced, so that neovascular endothelial cells of bone defect broken ends can be guided to directionally migrate to the high-concentration part of the central area of the repair membrane through chemotactic action, and the directional vascularization of bone tissues in a defect area from the two ends to the central area is accelerated; meanwhile, the newly generated blood vessel can be used as a stent to guide cells to quickly migrate and enter a fibrous membrane, so that bone tissue regeneration and repair are efficiently promoted.

This invention discloses a microfluidic chip for three-stage non-destructive sperm screening and its usage method. The chip includes a substrate, a sample inlet channel layer, a sample chamber layer, a recovery chamber layer, and a screening filter membrane. The sample inlet channel layer has an inlet channel and an overflow channel. The sample chamber layer has an annular sample chamber, an inlet orifice, and an overflow orifice. The recovery chamber layer is divided into an outer recovery chamber and an inner recovery chamber by a dike. An inducing agent patch is located in the center of the inner recovery chamber. The screening filter membrane is sandwiched between the sample chamber layer and the recovery chamber layer. In use, after injecting a sperm sample, initial motility screening is achieved through the screening filter membrane. Secondary chemotactic screening is completed through an inducing agent concentration gradient. Tertiary adaptive screening for swimming direction is completed using the chemotactic channel. Finally, high-quality sperm are obtained from the inner recovery chamber. This invention achieves multi-parameter graded screening, effectively avoiding sperm damage, improving sperm recovery quality and screening efficiency, and is applicable to various assisted reproductive technologies, helping to improve conception success rates.

The present application provides the use of chemokine CCL23 in the preparation of drugs for breast cancer immunotherapy, which relates to the fields of molecular biology and immunology. Regarding the use of chemokine CCL23 in the preparation of drugs for breast cancer immunotherapy, the chemokine CCL23 of the present application can induce the M1 polarization of TAMs in breast cancer, secrete cytokines such as IL-12, CXCL9, and CXCL10, promote the chemotaxis and function of Th1 cells and CD8+ T cells, enhance the formation of the anti-tumor immune microenvironment, and thus may serve as a new strategy for breast cancer immunotherapy.

The application discloses a di(2-ethylhexyl) phthalate degrading bacterium, a bacterial composition and application thereof. The degrading bacterium is named Sphingomonas parapaucimobilis GHQ28 and Stenotrophomonas maltophilia GHQ31. Research finds that GHQ28 and GHQ31 have chemotaxis to DEHP and downstream metabolites thereof, and there is a synergistic interaction between the two bacteria; the growth and DEHP degradation speed of the two strains under co-culture conditions are faster than that under single culture conditions. The bacterial composition composed of the two strains has a highest degradation rate of 99.2±0.59% to 400mg / L DEHP within 72h, compared with single culture, the total biomass is increased by 1.4-50.3 times, and the DEHP degradation rate is increased by 1.07-58.82 times. The application provides a new technical means for remediation of DEHP contaminated soil or water.

The invention provides a preparation process of a soothing and repairing type mask, and belongs to the technical field of cosmetics, an intelligent response type cell bionic delivery system is adopted, macrophage membrane vesicles serve as a carrier, a composite repairing kernel, epicatechin gallate, a neuropeptide P substance antagonist and idebenone are wrapped, and by means of the inflammation chemotaxis of a macrophage membrane, the soothing and repairing type mask is prepared. According to the present invention, the active component can actively target the inflammation region, the phase change release core is provided in the weakly acidic microenvironment with the pH value of 5.0-6.0, the targeting property and the permeation efficiency of the active component can be improved, the bottleneck of the traditional passive diffusion can be broken through, and compared with the conventional liposome or nanoparticle, the active component can be accurately delivered to the inflammation core region, the permeation rate can be improved by several times, and the carrier can directly reach the problem core region; according to the method, the utilization rate of effective components is greatly increased, a micro-ecology-immunity-nerve-physical barrier multi-dimensional and systematic synergistic repair network is constructed, full-path fundamental repair from immediate relieving to root strengthening is achieved, and immediate relieving and long-term barrier repair effects are provided.

The invention relates to an inflammatory environment induced overexpression 1L-10 engineered macrophage and a construction method and application thereof, and belongs to the field of biomedicine.The construction method includes the steps that a recombinant plasmid for expressing IL-10 is constructed with a genetic engineering method, and an RAW264.7 macrophage is jointly transfected with the constructed recombinant plasmid for expressing IL-10 and a Cas9 plasmid, so that the engineering macrophage with the inflammatory environment induced overexpression 1L-10 is obtained, and the engineering macrophage with the inflammatory environment induced overexpression 1L-10 is obtained. According to the method, the IL-10 gene is used for replacing the position of the IL-1 gene in the RAW264.7 macrophage, so that the RAW264.7 macrophage can express IL-10 under the induction of an inflammatory environment, the constructed engineered macrophage is injected into the abdominal cavity of a colitis model mouse in an intraperitoneal injection manner, the macrophage is positioned to the colitis disease part and secretes an anti-inflammatory cytokine IL-10 due to the inflammatory chemotaxis of the macrophage, and the anti-inflammatory cytokine IL-10 is obtained. The effect of relieving intestinal inflammation is achieved. According to the invention, the development of ulcerative colitis treatment technology is facilitated, and the application of a macrophage-based cell treatment method in treatment of digestive system diseases is promoted.

The invention discloses an application of a Pik3cb inhibitor in treatment of Sjogren's disease. Experiments prove that the Pik3cb inhibitor TGX-221 can reduce the number, activation and chemotaxis of NOD mouse B cells of a Sjogren's disease model mouse, plasma cell differentiation and antibody secretion, improve the immune microenvironment of the submaxillary gland of the Sjogren's disease model mouse, reduce the infiltration degree of submaxillary gland lymphocytes and promote the salivary secretion function; and after the Pik3cb is over-expressed, the symptom and pathology of the Sjogren's disease are aggravated. Therefore, the Pik3cb inhibitor can be used for treating the Sjogren's disease. The invention provides a new thought for research and development of medicines for treating the Sjogren's disease, and has important application value.

The invention provides a method for enhancing the tumor homing ability of TIL cells, and belongs to the technical field of biological medicine. The invention provides a method for effectively enhancing the homing ability of TIL cells, so that the chemotactic and enrichment potential of engineered TIL cell products to tumor sites is remarkably improved. The TIL cell preparation prepared on the basis of the method has higher tumor targeting performance, and a new solution is provided for overcoming the bottleneck that effector cells'reach 'tumor parts in an existing cell therapy. The effectiveness of single cell therapy is expected to be directly improved, and better cell products can be provided for synergistic combination with other therapies such as radiotherapy and chemotherapy, so that wider and more effective treatment choices are brought to patients with solid tumors.

The application provides a culture method and a verification method for evaluating a model of circadian rhythm, and belongs to the field of disorder model establishment and active substance function evaluation. By culturing nematodes with normal circadian rhythm and nematodes with circadian rhythm disorder, a model for evaluating circadian rhythm is established. The method is simple and easy to control, can more accurately simulate the influence of environmental factors on circadian rhythm, and lays a foundation for establishing a stable and repeatable evaluation model of circadian rhythm disorder. Whether the circadian rhythm of the nematodes is normal or not is effectively verified by the following method: a test plate is prepared, the nematodes with normal circadian rhythm or the nematodes with circadian rhythm disorder are placed in a test area; a fixed volume of an inducer is added at a fixed position, and the test plate is covered; nematode numbers in different areas of multiple test plates are counted at intervals of a fixed time; a chemotaxis index is calculated to verify the circadian rhythm.

This invention proposes a method for preparing a neutrophil membrane-like biomimetic nanoplatform, characterized by the following steps: Step 1: Synthesizing a 4-octyl itaconic acid-retinol prodrug (OR); Step 2: Preparing ORNPs; Step 3: Extracting dHL-60 cell membranes; Step 4: Assembling M@ORNPs to obtain core-shell structured M@ORNPs nanoparticles with a hydration kinetic diameter of approximately 108.2 nm, which constitute the biomimetic nanoplatform. The biomimetic nanoplatform possesses active chemotaxis and immune escape properties, enabling precise delivery of 4-octyl itaconic acid-retinol in sepsis-associated acute lung injury.

The application provides a kind of lipopeptide compound PW-1 and its preparation method and application in preventing and controlling bacterial wilt, belong to biological pesticide technical field;The PW-1 is prepared by fermentation, solvent extraction, acidification and reverse phase chromatography from Paenibacillus polymyxa (P. Paenibacillus polymyxa ) strain DW-7A;The core of the compound is that it plays a role through "anti-toxicity" mechanism, at the concentration without inhibiting the growth of Pseudomonas solanacearum (P. Ralstonia solanacearum ), can specifically interfere with the biosynthesis of pathogenic bacteria flagellum, cause its motility, chemotaxis and early infection ability to decrease significantly;Pot experiment proves that the relative control efficiency of PW-1 to tobacco bacterial wilt reaches 62.2%, and has no effect on the growth of soil bacillus at the concentration of up to 1000 μg / mL, shows efficient, targeted and environment compatible characteristics;The application provides a new candidate compound and key technical support for the development of green pesticide targeting flagellum.

The invention provides burkholderia as well as a composition, application and a using method thereof. Specifically, the invention provides burkholderia, and a composition containing the strain and / or metabolites, cultures, fermentation broth and / or extracts thereof. The burkholderia or the composition thereof can generate siderophores, induce plants or seeds thereof to generate systemic resistance to plant pathogens and reduce the chemotaxis of the plant pathogens to the plants, so that the prevention and treatment of plant diseases and insect pests are realized; in addition, plant root development can be promoted, a nitrogen fixation effect is provided, a phosphate solubilizing effect is provided, indoleacetic acid is generated, weeds are removed, and therefore plant growth is promoted.

This invention belongs to the field of pharmaceutical technology and specifically discloses the use of CL in a drug for the treatment and prevention of Alzheimer's disease and Parkinson's disease. Studies have demonstrated that CL can significantly improve the motility and chemotaxis of Caenorhabditis elegans with Alzheimer's disease, significantly extend the lifespan of Caenorhabditis elegans with Alzheimer's disease, and significantly restore damaged neurons in Caenorhabditis elegans with Parkinson's disease, suggesting that CL has the potential to be used in the development of therapeutics for neurodegenerative diseases.

The invention provides a toxoplasma gondii attenuated strain RH [delta] ku80 [delta] hxgprt capable of enhancing a macrophage function. The TUB1 is TIR1-3FLAG, and the SAG1 is CAT; [delta] OMPDC:: dhfr-ts: dhfr, [delta] OMPDC:: dhfr; [delta] UP:: dh fr-ts: hxgprt ([delta] OMPDC [delta] UP). The low virulent strain delta OMPDC delta UP is obtained by knocking out OMPDC and UP genes from a TIR1 insect strain by using a CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-associated 9) technology. The strain can effectively enhance macrophage functions including phagocytic ability, proinflammatory factor secretion and the like. The insect strain construction process comprises plasmid construction, homologous template amplification and positive insect strain screening and identification. In order to systematically evaluate the immune regulation efficiency, the regulation effect of the attenuated strain on proliferation, migration, chemotaxis, active oxygen, phagocytic function and apoptosis of macrophages is quantitatively analyzed by adopting multi-dimensional analysis technologies such as a CCK-8 method, a cell scratch experiment and a Transwell migration model. Furthermore, the mRNA expression level of inflammatory cytokines after polypide infection with macrophages is determined through qPCR, and a theoretical basis and technical support are provided for clarification of an immunoregulation network of toxoplasma gondii-host interaction and development of vaccine design based on low virulent strains.