150 results about "Angiogenesis growth factor" patented technology

The invention discloses a filling and repairing agent for promoting angiogenesis and a preparation method thereof, the filling and repairing agent is composed of a vascular endothelial cell supernatant, acellular matrix particles, a suspending aid and a protective agent, the vascular endothelial cell supernatant is prepared by inducing vascular endothelial cells into a tube under a low-oxygen three-dimensional condition, collecting a cell culture solution, centrifuging and concentrating; the acellular matrix particles are obtained by acellular tissue matrix, shearing, freeze-drying, liquid nitrogen grinding and collecting; according to the filling repairing agent for promoting angiogenesis, the vascular endothelial cell supernate and the acellular matrix particles are combined, the filling repairing agent contains high-concentration angiogenesis-related factors and a stable three-dimensional bionic scaffold structure, function complementation is achieved through the synergistic effect of the bioactive factors and the bionic scaffold, and the filling repairing agent for promoting angiogenesis is obtained. Therefore, the filling repair agent which can promote angiogenesis, construct a physical support framework and promote tissue repair and regeneration capacity is innovatively obtained.

The invention belongs to the technical field of biological materials, and particularly relates to nano composite hydrogel as well as a preparation method and application thereof. In the preparation process of the hydrogel, preparation of gelatin hydrazide and preparation of pullulan oxidation are sequentially completed, then preparation of active component loaded PLGA nanoparticles and self-assembly coating with metal-polyphenol are completed, and finally, the metal-polyphenol coated PLGA nanoparticles are mixed with gelatin hydrazide and pullulan oxidation to form gel in situ. The hydrogel has the characteristics of injectability, self-healing, wet adhesion and the like, the metal-polyphenol layer has the effects of resisting inflammation, resisting oxidation and promoting angiogenesis and generates photo-thermal response to near infrared rays, on-demand controllable release of the drug-loaded nanoparticles is achieved, the composite hydrogel can improve the wound surface microenvironment, promote angiogenesis and inhibit inflammation and scar formation, and the drug-loaded nanoparticles can be applied to wound healing. The ointment is suitable for burns, scalds and various complex wounds.

The application provides a thermoelectric hydrogel composite dressing for promoting repair of diabetic ulcers and loading of targeted modified exosomes and a preparation method, which comprises the following steps: preparing methacrylated hyaluronic acid; preparing polyether F127 diacrylate; co-incubating exosomes with palmitic acid grafted targeting peptides to prepare targeted modified exosomes; mixing the methacrylated hyaluronic acid, the polyether F127 diacrylate, a photosensitizer, the targeted modified exosomes and an electrolyte, and crosslinking under ultraviolet light to form a composite gel. The composite dressing is a novel composite dressing for thermoelectric stimulation and exosome treatment. On one hand, the dressing generates a wireless and passive electric field to simulate the endogenous electric field in the wound, promote directional migration of skin cells, accelerate reepithelialization and dermal formation; on the other hand, the dressing releases exosomes to precisely target blood vessels of a wound lesion, reverse endothelial dysfunction in a high-sugar environment and promote angiogenesis, and effectively solves the problems in repair and regeneration of diabetic ulcer wounds.

The invention provides an application of a super enhancer inhibitor JQ-1 in preparation of peripheral artery disease related drugs, relates to the technical field of biomedicine, and discloses a core effect of the super enhancer inhibitor JQ-1 in peripheral artery disease ischemia repair. The traditional Chinese medicine composition can significantly accelerate blood flow recovery of ischemic limbs, up-regulate mRNA and protein expression of a vascular marker CD31 in gastrocnemius muscle tissues, effectively increase vascular density and promote angiogenesis. Aiming at the defects of the existing treatment means in the aspect of ischemic tissue angiogenesis, the application provides a brand new molecular targeted treatment scheme for high-risk groups such as patients with diabetes-related peripheral artery diseases, and can significantly reduce the risk of lower limb amputation and the incidence rate of related cardiovascular adverse events such as coronary heart disease and stroke; the technical blank of specific targeted therapy of peripheral artery diseases is filled, a solid scientific basis and a key direction are provided for research and development of novel drugs, and the specific targeted therapy method has extremely high clinical application value and wide research and development prospects.

The invention relates to the technical field of medical instruments, in particular to a photo-piezoelectric hydrogel dressing for wound repair. The hydrogel dressing realizes self-energized micro-electrical stimulation and anti-oxidation-antibacterial-anti-inflammatory integrated treatment through'energy-drug 'double closed-loop synergy: the PVDF / ZnO nanofiber on the upper layer can convert human body micro-motion and external light into micro-electrical stimulation to promote cell migration, proliferation and angiogenesis; the curcumin at the lower layer cooperates with the nano-silver to block the'oxidative stress-inflammation-infection 'vicious circle of chronic wounds. The dressing is soft and fit, does not need an external power supply, is excellent in biocompatibility, provides a self-driven multifunctional nursing scheme for refractory wounds, and has wide clinical popularization and market application prospects.

According to the application of the dipsacus asper saponin VI in regulating and controlling the differentiation of the surface stem cells, the brand new pharmacological action of the dipsacus asper saponin VI in targeted promotion of the differentiation of the epidermal stem cells is found for the first time, the cognitive limitation of the prior art is broken through, a brand new target spot and direction are provided for research and development of innovative drugs for wound repair, and the application of the dipsacus asper saponin VI in regulating and controlling the differentiation of the epidermal stem cells is realized. A medicine containing teasel saponin VI is adopted, epidermal stem cells are taken as a core target, wound reepithelialization is accelerated by promoting directional differentiation of the epidermal stem cells, the mechanism is clear, the targeting property is high, a core link capable of directly hitting wound healing is adopted, epidermal stem cell differentiation is regulated in a targeted manner, and meanwhile, the medicine has the synergistic effects of resisting inflammation and promoting angiogenesis; the obvious application potential is realized on refractory wounds such as diabetic feet and pressure sores.

The invention relates to antioxidant sonodynamic hydrogel as well as a preparation method and application thereof, the yield and sonodynamic performance of ROS (reactive oxygen species) can be improved, and the prepared hydrogel can realize efficient antibiosis in the early stage of infection, exert antioxidant and repair promoting effects in the later stage of infection, and can promote angiogenesis and collagen deposition, optimize inflammation and immune microenvironment, improve the immunity of the ROS and improve the ROS yield and the sonodynamic performance of the ROS. The dynamic balance of antibiosis, antioxidation and healing promotion is realized, and the treatment effect is comprehensively improved.

The invention discloses a preparation method and application of dual-network piezoelectric induction hydrogel with a piezoelectric effect, and the method comprises the following steps: taking ACG and GelMA as base materials, taking MBA as a cross-linking agent, cooperating with a photoinitiator, and carrying out free radical polymerization to form a precursor solution; and adding BTO nanoparticles, mixing, and curing to obtain the hydrogel. According to the hydrogel disclosed by the invention, the mechanical property and the structural stability of the material are remarkably enhanced through a double-network structure, and the defects that traditional hydrogel is prone to excessive deformation and insufficient in mechanical property are overcome; by introducing a functional component with piezoelectric property, the material can generate a stable electric signal under the action of external force, the local electrophysiological environment of a wound surface is improved, cell proliferation, migration and angiogenesis are promoted, and tissue repair is accelerated; the anti-infection function can be further achieved while electrical regulation and control are achieved, the limitation of a single drug loading or passive protection strategy is avoided, drug resistance and toxic and side effect risks are reduced, and the synergistic effect of antibiosis and regeneration is achieved.

The present invention discloses a hydrogel and its application in ischemic heart disease, relating to the technical field of myocardial infarction repair. The present invention provides a multifunctional hydrogel with injectability, enabling in-situ injection. This hydrogel combines the comprehensive characteristics of improving the conductivity of the infarcted area, generating oxygen, scavenging ROS, promoting M2 polarization of macrophages, and promoting angiogenesis. Its excellent performance is outstanding in alleviating MI / RI, improving the local microenvironment, and enhancing myocardial repair function, providing a new direction for the treatment of heart injury. At the same time, the CSA-δ hydrogel formed after loading drugs has the ability of drug sustained release, which enables the drugs to be released continuously and stably, thereby prolonging the drug action time.

The invention discloses a GLP-1 (32-36) engineered exosome and application of the GLP-1 (32-36) engineered exosome in drugs and instruments for preventing and / or treating diabetic lower limb arterial lesion. The preparation process and related characteristics of the GLP-1 (32-36) engineered exosome are respectively as shown in figures (1) and (2). The exosome is prepared by treating human pluripotent stem cells by using GLP-1 (32-36). The GLP-1 (32-36) engineered exosome has relatively high activity in plasma, and the enterotrypsin synthetic peptide can effectively improve the yield of the exosome and further optimize the function of the exosome, so that a stronger therapeutic effect of promoting angiogenesis after ischemia is exerted. The GLP-1 (32-36) engineered exosome disclosed by the invention also has safety and can be used for a treatment strategy of diabetic lower limb artery complications.

The present application relates to the technical field of wound repair, and provides a traditional Chinese medicine composition for promoting wound healing and a preparation method thereof.The traditional Chinese medicine composition comprises a first traditional Chinese medicine composition and a second traditional Chinese medicine composition which are applied to the acute phase and the repair proliferation phase.The former comprises naked flower purple perilla extract, nectarine leaf extract, purple perilla extract, dragon's blood micro powder, spironast extract, centella asiatica volatile oil, rhubarb extract, borneol, mussel mucin, medicinal glycerol and purified water.The latter comprises red algae extract, nectarine leaf extract, radix astragali extract, panax notoginseng total saponins, aloe extract, dendrobium officinale extract, sea buckthorn extract, pinctada martensii purified peptide, snail glycosaminoglycan, medicinal glycerol and purified water.The composition can play the roles of antibiosis, anti-inflammation, hemostasis, analgesia and astringency in the acute phase, and can promote angiogenesis, collagen synthesis and epithelial regeneration in the repair proliferation phase, so that the wound is rapidly repaired and healed.

The invention discloses a polysaccharide-tea polyphenol-metal compound, which is formed by dispersing a metal-tea polyphenol nano ligand in polysaccharide gel formed by covalent crosslinking of atractylodes macrocephala polysaccharide and sodium alginate, and the metal-polyphenol nano ligand is a nano complex formed by coordination of tea polyphenol and metal ions. The invention also discloses a preparation method and application thereof. The rhizoma atractylodis macrocephalae polysaccharide-sodium alginate-tea polyphenol derivative-metal composite system disclosed by the invention not only can improve the stability and release behavior of tea polyphenol in vivo, but also can regulate and control an immunometabolism microenvironment in a bone repair process, induce inflammation resolution and promote angiogenesis and bone matrix deposition, so that the bone repair effect is improved. Multiple synergistic effects of inflammation resistance, oxidation resistance, vascularization promotion and osteogenesis induction are realized, so that repair and regeneration of skull defects are remarkably accelerated, and a new intelligent immunometabolism regulation strategy is provided for repair of complex bone defects.

The invention discloses a preparation method of a pyridopyrimidine compound and application of the pyridopyrimidine compound in preparation of an asthma medicine. The pyridopyrimidine compound provided by the invention is applied to preparation of medicines for preventing / treating asthma, DPPO is applied to prevention and treatment of bronchial asthma, and a new medicine choice is brought to asthma patients. Experimental results show that the DPPO can obviously inhibit attack of asthma and secretion of related inflammatory factors such as IL-1beta, IL-4, IL-5, IL-6, IL-13 and IL-17A, has a relaxation effect on tracheal smooth muscles, has the effects of resisting angiogenesis, airway remodeling and inflammation, and achieves the effect of treating asthma. In addition, current researches show that the asthma pathogenesis is very complex, DPPO can obviously improve pathological changes of mouse lung tissues sensitized by house dust mite extract (HDM) and toluene diisocynate (TDI), and the DPPO has certain universality and can be speculated to have huge application potential in research and development of drugs for treating various asthma.

The application relates to the application of lycorine hydrochloride in the preparation of a medicine for inhibiting the proliferation of a neointimal in a blood vessel. Lycorine hydrochloride has the effect of inhibiting the proliferation of a neointimal in a blood vessel, and the effect is mainly realized by inhibiting the proliferation and migration of vascular smooth muscle cells. In the body, the results show that lycorine hydrochloride can significantly inhibit the intimal proliferation caused by left common carotid artery ligation, and in vitro experiments prove that lycorine hydrochloride can intervene in the proliferation, migration and phenotype transformation of VSMCs induced by PDGF-BB. Lycorine hydrochloride inhibits the proliferation, migration and phenotype transformation of VSMCs induced by PDGF-BB, and the proliferation of a neointimal in a blood vessel through a MAPKs signal path. The application provides data for preparing lycorine hydrochloride into a medicine for inhibiting the proliferation of a neointimal in a blood vessel, and is expected to be applied to the prevention and treatment of complications after percutaneous coronary intervention, such as restenosis or in-stent thrombosis, and can become a potential medicine for intervening in restenosis.

This application relates to the fields of biomedicine and wound repair technology, and particularly to the application of platelet exosomes in the treatment of diabetic wound healing. This application discloses the use of platelet exosomes in the preparation of products having one or more of the following functions: 1) prevention and / or treatment of diabetic foot; 2) promotion of wound healing and / or tissue repair; 3) shortening wound healing time; 4) promoting granulation tissue growth; 5) enhancing wound angiogenesis; 6) alleviating inflammatory responses; 7) inhibiting the expression of pro-inflammatory factors; 8) promoting the expression of anti-inflammatory factors; 9) reducing MMP-9 expression; and 10) increasing MMP-2 expression. It is particularly suitable for the treatment of diabetic foot ulcers, delayed wound healing, and related complications, and also covers the use of this product in the preparation of diabetic foot treatment drugs and medical dressings.

This invention discloses an intestinal fungus Saccharomyces cerevisiae M7015 and its applications belong to the field of microbial technology. Saccharomyces cerevisiae The accession number for M7015 is GDMCC No. 66195. This invention first demonstrated, using a mouse subcutaneous stroma embolism model, that the aforementioned strain can promote angiogenesis; secondly, using mouse and Bama miniature pig myocardial infarction models, it was found that the aforementioned strain can promote collateral angiogenesis in ischemic areas of the heart and improve cardiac function. This invention not only fully elucidates... Saccharomyces cerevisiae M7015 promotes the formation of collateral vessels in the heart and improves cardiac function, and also reveals the broad application prospects of this bacterium in the treatment of vascular diseases.

The present application relates to the application of CircRAB3IP in the preparation of a drug for promoting angiogenesis, and belongs to the technical field of biological medicine; the present application provides the application of CircRAB3IP in the preparation of a drug for preventing or treating heart failure; in the preparation of a drug for promoting angiogenesis; in the preparation of a heart failure prognosis diagnosis kit; and in the preparation of a myocardial hypertrophy prognosis diagnosis kit; the present application proves through in-vivo and in-vitro experiments that circRAB3IP can promote angiogenesis, and can effectively improve heart function disorder caused by high blood pressure mechanical stress, slow down cardiac remodeling, myocardial fibrosis, and reduce cardiac inflammation. The present application provides a new target and theoretical basis for the treatment of hypertensive cardiac remodeling.

The application is a preparation of a composite stem cell active biomaterial and its application in artificial skin, effectively solves the problems of high production cost, complex production process and non-use for active substance research of the existing method, and comprises a double-layer composite material, the lower layer is an active material layer in the form of hydrogel composed of active liposomes and stem cells, and the upper layer is an antibacterial layer; the preparation method of the thiolated hyaluronic acid / H active factor composite hydrogel is relatively mild, does not need to add a crosslinking agent and contains a large amount of active ingredients; by using the mechanism, the environment can induce angiogenesis through endothelial cells / fat stem cells combined with active factors and platelet lysate and rapidly construct microtissues in vitro, which is beneficial to the scaffold in large-scale cultivation of artificial skin basement layer in vitro to meet the needs of patients.

The invention relates to a preparation method and application of macroporous alanine modified nanocellulose-based dynamic hydrogel, and the preparation method comprises the following steps: S1, by taking alanine and nanocellulose as raw materials, synthesizing alanine modified nanocellulose through esterification reaction; s2, phenylboronic acid and beta-cyclodextrin are used as raw materials, and phenylboronic acid modified cyclodextrin is synthesized through a substitution reaction; and S3, introducing the Ala-CNFs into a polyvinyl alcohol and PBCD system, so as to obtain the macroporous alanine modified nano cellulose based dynamic hydrogel. The synthesis and preparation method of the hydrogel has the characteristics of being green and environment-friendly, alanine can be grafted on nano cellulose in an aqueous solution environment, and the problem that tissue regeneration is hindered due to the fact that an existing injectable hydrogel adhesive occupies the space of angiogenesis and tissue regeneration in the wound healing process can be effectively solved. The hydrogel has the characteristics of injectability, self-healing, shape adaptability, biological tissue adhesion, biodegradability, biocompatibility, hemostasis, and promotion of tissue regeneration and angiogenesis.

The invention relates to structures and preparation methods of nine novel guaiane type sesquiterpene dimer compounds in xylostemma oblongifolium, and applications of the dimer compounds in anti-angiogenesis and anti-tumor aspects. The structures of the compounds are as shown in Figure 1. The invention also relates to a preparation method of the dimer compounds, and an application of the dimer compounds in anti-angiogenesis and anti-tumor aspects. The compound provided by the invention has angiogenesis inhibition activity and can be used for development and application of antitumor drugs.

The application discloses a composite hydrogel based on urethral stem cell membrane sheet ECM and exosomes and a preparation method and application thereof, and relates to the technical field of tissue engineering, and in particular relates to a soluble ECM protein solution obtained by dissolving and enzymatically hydrolyzing urethral stem cell membrane sheet decellularized matrix (ECM) powder and mixing the soluble ECM protein solution with exosomes in a mass ratio of 3:1-10:1, and performing temperature-sensitive crosslinking in DMEM at pH 7.0 and 36-38 DEG C to form a hydrogel. The composite hydrogel based on urethral stem cell membrane sheet decellularized matrix (ECM) and exosomes integrates the cell activity of the urethral stem cell membrane sheet, the structural support of the decellularized matrix (ECM) and the signal regulation of the exosomes, constructs a three-level functional system, forms a synergistic repair effect, and is helpful to promoting angiogenesis in the urethra. Through covalent coupling of the decellularized matrix (ECM) fiber network and the surface protein of the exosomes, the intelligent release of the exosomes in an inflammatory environment is realized, and the composite hydrogel is used for urethral repair and angiogenesis promotion.

The invention discloses a bi-crosslinked chitosan derivative antioxidant hydrogel as well as a preparation method and application thereof. The structure of the hydrogel comprises chitosan modified by 2, 3-dihydroxybenzoic acid and phosphate, and the chitosan modified by 2, 3-dihydroxybenzoic acid and the phosphate are combined through electrostatic interaction; and in the hydrogel, a crystalline region exists in at least partial region of the 2, 3-dihydroxybenzoic acid modified chitosan. The hydrogel provided by the invention is excellent in biocompatibility, high in antioxidant stability and remarkable in blood coagulation activity, has multiple free radical scavenging ability, can inhibit macrophage M1 type polarization activity, promote angiogenesis and regulate granulation tissue regeneration, and finally realizes the effect of promoting chronic wound healing. The composition is applied to the field of chronic wound treatment, and can promote regeneration of granulation tissues of chronic wounds and angiogenesis by removing ROS (reactive oxygen species) on the wounds and regulating and controlling the oxidative stress level of the wounds, so that the healing process of the chronic wounds is accelerated.

The object of this invention is to provide novel pharmaceutical agents that can be used for various treatments. The pharmacological compositions of this invention are characterized by comprising extracellular vesicles derived from equine mesenchymal stem cells. The pharmacological compositions of this invention are tissue repair agents, anti-inflammatory agents, angiogenic agents, or wound healing agents.

The invention discloses an application of nano silicate in assisting fat transplantation. The application comprises the following steps: preparing the nano silicate into a silicate ion solution by adopting a serum-free culture medium, mixing the silicate ion solution with a fat aspirator, and performing transplantation injection, meanwhile, the invention discloses a fat graft obtained by mixing a silicate ion solution prepared from the nano silicate by adopting a serum-free culture medium with a fat aspirator, a preparation method of the fat graft, and application of the fat graft as a soft tissue filler. Compared with traditional fat transplantation, the fat graft provided by the invention has the advantages that by synergistically promoting angiogenesis, inhibiting inflammation / fibrosis and protecting early excessive apoptosis of fat cells due to ischemia and hypoxia, the long-term survival quality of transplanted fat can be remarkably optimized, the fat retention rate is increased to 60% or above, and the survival rate of the transplanted fat is increased to 60% or above. Wide application prospects are realized in the aspect of clinical fat transplantation.

The invention relates to the technical field of medicines, in particular to application of an AKT inhibitor targeting FOXO in preparation of a medicine for promoting myocardial ischemic tissue angiogenesis and application of the AKT inhibitor in preparation of a medicine for treating ischemic heart diseases. In the cell level, the AKT inhibitor Ipatasertib can promote migration and tube formation of the HUVEC, and when the 6 [mu] M of Ipatasertib acts on the HUVEC, the expression of AKT can be significantly reduced, and the phosphorylation level of FOXO3 can be inhibited. In an in-vivo experiment, compared with a model group, a mouse in an Ipatasertib intervention group has the advantages that the left ventricular ejection fraction of an echocardiogram is remarkably increased, the myocardial infarction area is remarkably reduced, and the fluorescence density of an angiogenesis marker CD31 + is remarkably increased, so that the Ipatasertib can promote angiogenesis of a myocardial ischemia region of the mouse with acute myocardial infarction and rescue myocardial injury of the mouse.

The application discloses a kind of compositions based on engineering stem cell exosome and its application in treating male sexual dysfunction.The composition includes mesenchymal stem cell exosome modified by genetic engineering, which displays bifunctional fusion polypeptide targeting TβRⅡ and LOXL2 on the surface. The fusion polypeptide integrates TβR-PEP with LOXL2-PEP through flexible linker, and introduces HIV-TAT penetrating peptide and self-assembly motif, forms nanoparticles to extend half-life. In vitro experiments show that the inhibition efficiency of the fusion polypeptide on TβRⅡ and LOXL2 is significantly better than that of single polypeptide, and can synergistically reverse fibrosis and promote angiogenesis. Animal experiments confirm that exosome loaded with fusion polypeptide significantly improves the erectile function of diabetic ED rats. The application provides a radical treatment scheme for male sexual dysfunction through double-target synergistic, long-acting delivery and multi-effect repair mechanism.

The invention relates to a construction method of a perfusion vascularized cervical cancer organoid chip, and relates to the technical field of biology. Comprising the following steps: S1, constructing a cervical cancer organ; s2, mixing the cervical cancer organ small cell cluster, the human umbilical vein endothelial cells and the human embryonic lung fibroblasts in proportion, and centrifugally gathering the mixed cells in a low-adsorption U-shaped plate to form a pre-vascularized cervical cancer organ; s3, re-suspending the pre-vascularized cervical cancer organoid and the human umbilical vein endothelial cells in the fibrous protein hydrogel, transferring the pre-vascularized cervical cancer organoid and the human umbilical vein endothelial cells into the organoid chip, and adding a vascularized cervical cancer organoid culture medium for culture, so that the endothelial cells form perfused blood vessels, and finally obtaining the perfused vascularized cervical cancer organoid chip. The prepared perfusion vascularized cervical cancer organoid can well solve the in-vitro vascularization problem of the organoid, simulates the in-vivo tumor angiogenesis process, is close to the in-vivo real tumor microenvironment, and provides technical support for exploration of cervical cancer disease mechanisms and related treatment.

The invention discloses a hydrogel dressing containing pearl sac liquid as well as a preparation method and application of the hydrogel dressing, and belongs to the technical field of medical dressings. The hydrogel dressing is prepared by taking polyvinyl alcohol (PVA) and chitosan (CS) as gel matrixes and loading Markov pearl sac liquid through an in-situ crosslinking technology. The hydrogel dressing is of a three-dimensional porous network structure and is excellent in water absorption performance, the mechanical property of the hydrogel dressing meets the wound repairing requirement, the bacteriostasis rate of the hydrogel dressing on common burn pathogenic bacteria such as staphylococcus aureus and pseudomonas aeruginosa exceeds 90%, and the hydrogel dressing is free of obvious cytotoxicity. When being applied to burn infection wounds, the medical dressing can effectively inhibit bacterial proliferation of the wounds, relieve inflammatory response, promote angiogenesis and tissue regeneration and remarkably improve the closure rate of the wounds, solves the problems that existing burn dressings are narrow in antibacterial spectrum, limited in repair promoting capacity and the like, provides a safe and effective novel dressing choice for clinical treatment of burn infection, and has wide application prospects. Meanwhile, the effective utilization of the martensitic pearl sac liquid is realized.

The invention discloses pigskin-derived recombinant lipid nanoparticles as well as a composite wound dressing and application of the pigskin-derived recombinant lipid nanoparticles. The nanoparticles are prepared by taking pigskin extracted lipid as a raw material and adopting an ethanol injection method, are rich in triglyceride and phosphatidylcholine, and have the biological activity of promoting fibroblast proliferation and migration. Furthermore, the active nano-particles are loaded in calcium ion cross-linked sodium alginate hydrogel to prepare the composite hydrogel dressing, and the dressing has injectability and shear thinning characteristics and can continuously release the active nano-particles. In-vitro experiments prove that the biocompatibility is good; animal experiments show that the traditional Chinese medicine composition can remarkably accelerate full-thickness skin defect healing and promote epidermis regeneration, ordered collagen deposition and angiogenesis. According to the invention, high-valued utilization of meat byproducts is realized, and a safe and efficient novel active dressing is provided for chronic wound treatment.

The invention discloses an antibacterial and healing-promoting traditional Chinese medicine hydrogel dressing as well as a preparation method and application thereof, and belongs to the technical field of medical materials. A low-temperature mixing and step-by-step cross-linking strategy is adopted, heat-sensitive traditional Chinese medicine components and high-temperature-resistant components are separated, and active substances are prevented from being damaged; an interpenetrating network of aluminum ion-phenolic hydroxyl coordination synergistic hydrogen bonds is designed to enhance the mechanical strength, and the porosity is regulated and controlled by a ternary solvent. The porosity is accurately regulated and controlled through a temperature control strategy, the stirring speed and the solution mixing ratio are adjusted to adapt to different wounds, and the production efficiency is high. Through gradient compatibility of the eight traditional Chinese medicine components, the dressing has broad-spectrum antibacterial property, the 24-hour bacteriostasis rate is larger than 70%, angiogenesis and fibroblast migration can be promoted, and the wound healing time is shortened. High-temperature damage is avoided by adopting a specific process, the encapsulation efficiency of the traditional Chinese medicine extract reaches 85%-92%, and the medicine is slowly released within 72 hours. The method is simple in process, controllable in cost and suitable for industrialization, and the dressing is suitable for complex wound treatment and large in clinical transformation potential.