4008 results about "Tyrosine" patented technology

Compounds that are covalent inhibitors of Src homology region 2 (SH2)-containing protein tyrosine phosphatase 2 (SHP2); compositions comprising same; and their use for treating cancers.

The invention discloses red ginseng fermentation liquor with high rare saponin content and functional bioactivity and a preparation method thereof, and the preparation method comprises the following steps: mixing red ginseng powder with water to obtain red ginseng mixed liquor; cellulase is added into the red ginseng mixed solution for enzymolysis, and red ginseng enzymatic hydrolysate is obtained; or heating the red ginseng liquid, and performing hot water extraction to obtain a red ginseng extracting solution; heating the red ginseng extracting solution to be boiled, and after sterilization is finished, adding lactic acid bacteria powder for fermentation after the red ginseng solution is cooled, so as to obtain red ginseng fermentation liquor; and carrying out solid-liquid separation on the fermentation broth to obtain a fermentation supernatant, and sterilizing the red ginseng fermentation broth to obtain the sterilized red ginseng fermentation broth. According to the preparation method disclosed by the invention, the content of total saponins in the red ginseng fermentation liquor is increased, the variety and content of rare saponins can be increased, and in addition, the prepared red ginseng fermentation liquor has relatively high antioxidant activity, tyrosinase inhibition rate activity, leukoproteinase inhibition activity and hyaluronidase inhibition activity; wide application prospects and market potentials are realized.

This disclosure provides a method for treating a subject afflicted with a lung cancer, which method comprises administering to the subject therapeutically effective amounts of: (a) an anti-cancer agent which is an antibody or an antigen-binding portion thereof that specifically binds to a Programmed Death-1 (PD-1) receptor and inhibits PD-1 activity; and (b) another anti-cancer agent. The other anti-cancer agent can be a platinum-based doublet chemotherapy, an EGFR-targeted tyrosine kinase inhibitor, an anti-VEGF antibody, an anti-Cytotoxic T-Lymphocyte Antigen-4 (CTLA-4) antibody, or any other therapy used to treat lung cancer in the art or disclosed herein.

The invention discloses an up-conversion nanoprobe for detecting tyrosinase through dual-signal output and application of the up-conversion nanoprobe, and relates to the technical field of nano biological materials, the up-conversion nanoprobe comprises UCNPs, tyramine and iron ions; the UCNPs has a core-shell structure, beta-NaYF4: Yb, Er is taken as a core, and NaYF4 is taken as a shell; according to the up-conversion nanoprobe disclosed by the invention, by integrating the advantages of a fluorescence determination method and a colorimetric method, the dual-signal self-calibration capability is formed; according to the method, the UCL signal excited by near-infrared light is utilized, the detection precision is greatly improved, and an intuitive and convenient method is provided for detection of tyrosinase through colorimetric reaction; and a dual-signal output detection mode effectively relieves signal fluctuation, so that the accuracy and reliability of tyrosinase analysis are ensured.

The invention provides glabridin nano-vesicles based on glycyrrhizic acid wrapping, a preparation method of the glabridin nano-vesicles and application of the glabridin nano-vesicles in preparation of whitening and skin care cosmetics, and relates to the technical field of cosmetics. According to the glabridin nano-vesicle based on glycyrrhizic acid wrapping, glabridin is carried by glycyrrhizic acid, glycyrrhizic acid serves as a carrier, the solubility of glabridin can be improved, the skin transmittance of glabridin can be increased, the anti-inflammatory effect of glabridin can be achieved, then the effect of one stone for three birds is achieved, the characteristic and advantage of integration of medicine and auxiliary materials are achieved, and the glabridin nano-vesicle based on glycyrrhizic acid wrapping has good application prospects. The tyrosinase inhibition activity of the glabridin is greatly improved. Meanwhile, the glabridin nano-vesicles wrapped based on glycyrrhizic acid have the advantages of high encapsulation efficiency, large drug loading capacity, good stability and high safety, can effectively promote the whitening factor glabridin to penetrate through the skin cuticle to enter the skin deep tissue, can stay in the skin at a high concentration for a long time, and can continuously play a whitening role.

The invention discloses an anti-aging composition containing ergothioneine, glutathione and fish collagen, relates to the technical field of anti-aging compositions, and aims to solve the technical problems that a traditional anti-aging composition lacks attention to the aspects of cell energy metabolism, barrier repair and the like, and the overall skin improvement effect is not obvious. The ergothioneine is used as a natural antioxidant, and the content of the ergothioneine is 0.01-5wt%; the ergothioneine participates in a synergistic effect of an intracellular antioxidant enzyme system, so that tyrosinase activity is effectively inhibited, melanogenesis is reduced, and DNA and protein are protected from oxidation damage; in a simulated strong ultraviolet environment, compared with skin cells not using the ergothioneine-containing product, the content of free radicals in the cells is reduced by 30% after the ergothioneine-containing product is used; the ergothioneine can also regulate a cell signal channel, promote cell metabolism and maintain normal growth of cells. The mask has the advantages of accelerating skin metabolism and improving the self-repairing ability of the skin.

The invention relates to a medicinal and edible composition with anti-aging and whitening effects and application of the medicinal and edible composition. The composition comprises a raspberry extract, a guava extract, a rose extract and a liquorice extract. According to the composition, active ingredients of natural plants are utilized, and the anti-aging effect is achieved by inhibiting the activity of collagenase and elastase and promoting the generation of collagen and elastase; by inhibiting the activity of alpha-glucosidase and directly inhibiting generation of AGEs, aging caused by skin saccharification is prevented; the whitening effect is achieved by removing DPPH free radicals, inhibiting tyrosinase activity and reducing melanogenesis; according to the composition, through compatibility in a specific proportion, synergistic interaction is achieved, the whitening and anti-saccharification effects are further improved while the anti-aging effect is achieved, and the skin problem is solved from multiple dimensions. The four natural components used for the composition all derive from homology of medicine and food, no organic reagent is contained in the extraction process, and the composition is green, environmentally friendly, safe and reliable.

The invention provides application of tageteoside in resisting esophageal squamous cell carcinoma. The invention finds that tyrosine 174, 302, 328 sites of fructose diphosphate aldolase (ALDOA) are highly expressed in esophageal squamous cell carcinoma tissues and are in negative correlation with prognosis of esophageal squamous cell carcinoma patients; the tageteoside can play a role in resisting esophageal squamous carcinoma, and can inhibit activation of phosphorylation sites of ALDOA tyrosine 174, 302 and 328 in esophageal squamous carcinoma cells in a dose-dependent manner. Therefore, the tageteoside is an effective medicine for resisting esophageal squamous carcinoma.

The invention provides application of UBR5 in preparation of drugs for treating pigmented skin diseases, and further the pigmented skin diseases comprise various types of pigmented nevus (mainly congenital pigmented nevus and megalous with the risk of malignant transformation). Various pigmented spots (mainly chloasma, freckles, Taitian nevus, post-inflammation hyperpigmentation and the like) and hypopigmentation / depigmentation spots (mainly leucoderma, post-inflammation hypopigmentation and the like). Experimental results show that UBR5 is highly expressed in disease tissues such as pigmented nevus. The UBR5 expression level and the melanogenesis ability are in significant positive correlation, targeted knockout / knockdown of UBR5 in melanocytes can significantly inhibit the expression level of melanogenesis related genes such as MITF, TRP1, TRP2, TYR and other proteins, the melanin content of melanocytes and tyrosinase activity, vice versa, and the melanogenesis ability of melanocytes after overexpression of UBR5 is significantly increased.

The invention relates to the technical field of genetic engineering, and particularly discloses a genetic engineering bacterium for producing melanin as well as a construction method and application of the genetic engineering bacterium. According to the application, the yield of the melanin can be effectively increased by modifying a metabolic pathway of the genetically engineered bacterium, and the yield of the melanin reaches 6.2 g / L and 18.5 g / L respectively through shake-flask culture and 5L fermentation tank culture. Compared with the prior art, the yield of melanin produced by the genetically engineered bacterium is improved by 30% at the shake flask fermentation level, the fermentation yield of a fermentation tank is improved by 4.3 times, in addition, expensive tyrosine is changed into glucose as a fermentation precursor substance, the production cost is greatly reduced, and an efficient and feasible solution is provided for large-scale production of melanin.

The invention relates to the technical field of deep processing of phyllanthus emblica, in particular to phyllanthus emblica fermentation liquor and a preparation method thereof. The preparation method comprises the following steps: uniformly mixing crushed phyllanthus emblica pulp with water; after sterilization, adding lactic acid bacteria mixed powder for fermentation treatment; cellulase is added into the fermentation liquor for hydrolysis, and hydrolysate is obtained after enzyme deactivation; the hydrolysate is subjected to extraction treatment and solid-liquid separation, and the phyllanthus emblica fermentation liquor is obtained. By adopting the method disclosed by the invention, 107.11 mg of gallic acid, 360.76 mg of polyphenol and 65.56 mg of flavone can be extracted from each gram of the phyllanthus emblica raw material respectively, and the contents of gallic acid, polyphenol and flavone are increased by about 2.1 times, 4.5 times and 1.7 times respectively compared with those of gallic acid, polyphenol and flavone which are not subjected to fermentation treatment; the phyllanthus emblica fermentation liquor has alpha-glucosidase inhibitory activity, xanthine oxidase inhibitory activity, pancreatic lipase inhibitory activity, tyrosinase inhibitory activity and antioxidant activity; the phyllanthus emblica fermentation liquor is good in taste and flavor, and has the functions of reducing blood sugar, blood fat and uric acid and resisting oxidation.

The embodiment of the invention provides a tyrosine ammonia lyase mutant and application thereof in synthesis of p-coumaric acid. The mutant is subjected to any one of the following amino acid mutations on an amino acid sequence as shown in SEQ ID NO. 2: F497I, F497I + L248M, F497I + L248M + M4T, and L248M + F497I + M4T + V482L. According to the embodiment of the invention, an amino acid sequence as shown in SEQ ID NO. 2 is taken as a template, a key active site of the template is subjected to mutation transformation, a mutant library is constructed, and a series of mutants with remarkably improved catalytic activity and / or conversion rate are screened; the tyrosine ammonia lyase mutant provided by the embodiment of the invention can selectively catalyze L-tyrosine to generate p-coumaric acid, and has excellent yield and / or higher yield.

The invention relates to the technical field of carbon black, in particular to a preparation process of high-adsorbability carbon black, which comprises the following steps: performing pore-forming treatment on carbon black to obtain a porous carbon black material, then treating the porous carbon black material with a first mixed amino acid solution to obtain a carbon black material loaded with a first mixed amino acid adsorption layer, and drying the carbon black material to obtain the high-adsorbability carbon black. And treating the carbon black material with a second mixed amino acid solution to obtain a carbon black material loaded with a second mixed amino acid adsorption layer, and treating the carbon black material loaded with mixed amino acids with laccase and tyrosinase. The carbon black product obtained by the preparation process has high adsorbability, and is used for treating metal ions in wastewater, so that the removal rate of the metal ions is greatly improved.

The embodiment of the invention provides a method for obtaining a tyrosine ammonialyase mutant based on high-throughput screening, the tyrosine ammonialyase mutant and application of the tyrosine ammonialyase mutant. The mutant is subjected to any one of the following amino acid mutations on an amino acid sequence as shown in SEQ ID NO.2: A245G + A247T, and D192V + A245G + A247T. According to the embodiment of the invention, an amino acid sequence as shown in SEQ ID NO.2 is taken as a template (basis), a key active site of the template is subjected to mutation transformation, a mutant library is constructed, and a series of mutants with remarkably improved catalytic activity and / or conversion rate are screened; the tyrosine ammonia lyase mutant provided by the embodiment of the invention can selectively catalyze L-tyrosine to generate p-coumaric acid, and has excellent yield and / or higher yield, which indicates that the mutant has more significant application prospects in catalysis of L-tyrosine to generate p-coumaric acid.

The invention belongs to the field of medical chemistry, and particularly discloses a protein tyrosine phosphatase inhibitor, a pharmaceutical composition and application of the protein tyrosine phosphatase inhibitor, and the protein tyrosine phosphatase inhibitor is a compound shown in a general formula (I) or pharmaceutically acceptable salt of the compound. The invention also discloses a method for using the protein tyrosine phosphatase inhibitor to treat cancers, metabolic diseases and other related diseases. # imgabs0 #

The invention provides a mulberry leaf extract as well as a preparation method and application thereof in cosmetics, and relates to the technical field of fermentation and extraction of mulberry leaves. The preparation method of the mulberry leaf extract comprises the following steps: S1, inoculating a culture medium with compound bacteria to obtain a culture solution; s2, adding a mulberry leaf sample into the culture solution for fermentation, and feeding supplementary materials in the fermentation process to obtain fermentation liquor; s3, sterilizing, filtering, centrifuging and concentrating the fermentation liquid to obtain a crude extract; and S4, carrying out aqueous two-phase extraction, extraction, ultrafiltration and drying on the crude extract to obtain the mulberry leaf extract. The mulberry leaf extract prepared by the method has high contents of flavone, chlorogenic acid and peptide; the effects of inhibiting tyrosinase and removing DPPH free radicals are good.

Provided are a whitening and freckle-removing composition, a gel, a preparation method therefor, and a use thereof, relating to the technical field of skin-care products. The whitening and freckle-removing composition comprises tranexamic acid, nicotinamide, 4-butylresorcinol, licorice extract, 3-o-ethyl ascorbic acid, and glutathione. For the whitening composition, by inhibiting the activity of tyrosinase, blocking the transfer of melanin from melanocytes to keratinocytes, and inhibiting the formation and accumulation of melanin by means of multiple mechanisms including oxidation resistance, and by means of the synergistic action of all components, cosmetic benefits of whitening and freckle-removing are achieved. The whitening and freckle-removing gel has a simple preparation method, has a mild and non-sensitizing formula, is safe and non-irritating, and is suitable for large-scale production and application.

The invention discloses a method for fermenting mulberry leaves through compound probiotics, a mulberry leaf fermentation product and application, and relates to the technical field of fermentation. The fermentation method comprises the following steps: carrying out compound fermentation on mulberry leaves by adopting staphylococcus xylosus and lactobacillus plantarum according to a volume ratio of (1-3): (1-3), wherein the total inoculum size is 2-10% of the volume of a fermentation culture medium; the mulberry leaf fermentation product is prepared by the method. The invention also provides application of the mulberry leaf fermentation product in preparation of whitening cosmetics and / or skin care products. The preparation method has the beneficial effects that the two probiotics including the lactobacillus plantarum and the staphylococcus xylosus are used for performing compound fermentation on the mulberry leaves, and the lactobacillus plantarum and the staphylococcus xylosus can achieve a synergistic effect during compound fermentation of the mulberry leaves, so that active substances for inhibiting tyrosinase in the mulberry leaves can be effectively released; active substances are all retained in the fermentation product, and the inhibition rate of the fermentation product on the tyrosinase activity is remarkably increased.

The utility model provides a micro-fluidic chip and a micro-fluidic system, a micron-sized main channel is arranged in the micro-fluidic chip, and a sample to be detected is introduced into a generation area of the main channel to form a large number of micro-droplets. The micro-droplets serve as a reaction container to react with tyrosine and tyrosinase which are guided into the reaction area of the main channel, and finally absorbance testing can be conducted on the micro-droplets in the detection area of the main channel. The micro-droplets are small in volume, have high surface area to volume ratio, and can form a reaction container for efficient reaction. The microfluidic system can rapidly and accurately measure to-be-detected samples with different concentrations, and has the advantages of low reagent consumption, energy conservation, environmental protection, small required detection space, large-scale parallel operation and the like.

The invention belongs to the technical field of medicines, particularly relates to application of deaminotyrosine in preparation of a medicine for treating cardiomyopathy caused by anthracycline medicines, discovers that DAT can relieve cardiotoxicity caused by DOX to a great extent, and can provide a new thought for recovery of patients after chemotherapy and expansion of clinical application of DOX.

Disclosed are compounds having PTPN2 inhibitory effects and a use thereof. Specifically, disclosed are a class of compounds having protein tyrosine phosphatase inhibitory effects or pharmaceutically acceptable salts thereof, as well as a use thereof for treating and / or preventing diseases or conditions related to abnormal expression of protein tyrosine phosphatase. The compounds are represented by formula (I).

The invention relates to the field of polypeptide compositions, in particular to a polypeptide metal chelate and application thereof in the field of cosmetics, and the polypeptide metal chelate is formed by chelating a polypeptide compound and a metal ion compound. The structure of the polypeptide compound is [gamma-Glu] n-AA, n is greater than or equal to 1, and AA is selected from glycine, alanine, valine, leucine, isoleucine, methionine (methionine), proline, tryptophan, serine, tyrosine, cysteine, phenylalanine, asparagine, glutamine, threonine, asparaginic acid, glutamic acid, lysine, arginine or histidine; the polypeptide chelated zinc prepared by the invention has remarkable oil control and anti-inflammatory effects, and can be compounded with other raw materials to form cosmetics with oil control and acne removal effects.

The invention discloses polypeptide hydrogel based on oxidized ganoderan and application. The polypeptide hydrogel is simple in preparation method, rapid in gelling, good in stability and capable of resisting oxidation activity and inhibiting tyrosinase activity. The polypeptide hydrogel provides a physical barrier for the bioactive peptide, so that the influence of the gastrointestinal tract environment is overcome, 71% of polypeptide is released in the intestinal tract, the bioavailability of the peptide is improved, and the problem that the use condition of the polypeptide is limited is effectively solved.

The invention relates to a multi-effect bionic liposome transdermal repair carrier and a preparation method and application thereof.The multi-effect bionic liposome transdermal repair carrier comprises active ingredients and a nano-carrier, and the active ingredients comprise sialic acid, saccharomycetes / rice fermentation product filtrate, arginine / lysine polypeptide and vitamin E in the mass ratio of (1-10): (2-8): (0.1-1): (0.5-4); the nano-carrier is prepared from the following raw materials: phospholipid, an emulsifier, polyhydric alcohol and water. Compared with the prior art, the anti-aging, whitening and moisturizing composition has the advantages of enhancing skin moisturizing and anti-oxidation effects, promoting collagen synthesis and inhibiting tyrosinase to achieve remarkable anti-aging, whitening and moisturizing effects and the like.

The invention provides an anti-aging and whitening pyracantha fortuneana fruit extract prepared by adopting a supramolecular solvent and a preparation method of the pyracantha fortuneana fruit extract, and relates to the technical field of biological extraction. The preparation method comprises the following steps: by taking betaine as a hydrogen bond acceptor and 1, 3-propylene glycol as a hydrogen bond donor, mixing and self-assembling the betaine and the 1, 3-propylene glycol to form a supramolecular solvent; and the pyracantha fortuneana fruits are extracted by adopting the supramolecular solvent. The pyracantha fortuneana fruit active ingredients are extracted by adopting the supramolecular solvent which is harmless to the skin, so that the irritation of the solvent to the skin can be avoided, the supramolecular solvent can be specifically combined with the active ingredients in the pyracantha fortuneana fruit, and the purity and activity of the active ingredients are improved. The pyracantha fortuneana fruit extract extracted by adopting the supramolecular solvent has better oxidation reduction resistance; generation of non-enzymatic glycosylation intermediate products is inhibited, and saccharification and aging resistance are facilitated; the red blood cell oxidative damage is improved, and the effects of resisting oxidation and relieving are achieved; the whitening mask has an inhibiting effect on elastinase and tyrosinase, and can resist aging, whiten skin, relieve facial pigmentation and brighten the skin.

The invention relates to application of a tea exosome in preparation of a medicine for improving skin pigmentation, the tea exosome is creatively extracted and purified, and the tea exosome can effectively inhibit proliferation of melanoma cells and inhibit expression of melanin generation related protein by scavenging free radicals and inhibiting tyrosinase activity, so that the effect of improving skin pigmentation is achieved. The intracellular melanin removal is promoted. In a mouse in-vivo experiment, after the tea exosome is applied, it is observed that the skin epidermis thickness of a mouse is reduced, the dermis thickness is increased, collagen arrangement is tighter and more ordered, the content is increased, and meanwhile generation of inflammatory factors is reduced. The tea exosome can significantly increase the content of a melanin autophagy key factor LC3B and reduce the content of P62 to promote melanin autophagy, and has good percutaneous infiltration capacity, thereby playing a role in improving skin pigmentation.

The invention provides an application of N-trans-p-coumaroyl tyramine or a derivative thereof in preparation of a skin whitening product, and relates to the technical field of biology, and the application of the N-trans-p-coumaroyl tyramine or the derivative thereof in preparation of cosmetics for skin whitening or drugs for skin whitening is as follows: (I) the N-trans-p-coumaroyl tyramine or the derivative thereof is applied to preparation of cosmetics for skin whitening or drugs for skin whitening; (2) inhibiting melanogenesis in a non-diagnostic and therapeutic manner; (3) preparing a melanin inhibitor; (IV) inhibiting the tyrosinase activity of the subject in a non-diagnostic and therapeutic manner; or (V) preparing the tyrosinase inhibitor. According to the invention, a natural compound N-trans-p-coumaroyl tyramine which is derived from a traditional Chinese medicine and has a whitening effect is found, and a new functional component with a whitening effect is provided for the skin beautifying industry.

Provided herein are inhibitors of receptor tyrosine kinase effector, RAF, pharmaceutical compositions comprising said compounds, and methods for using said compounds for the treatment of diseases.

The invention relates to talaromyces purpurogenus D2 and application thereof, and belongs to the technical field of microorganisms. The talaromyces purpurogenus D2 disclosed by the invention has the preservation number of GDMCC (Graphics Decific Microorganism Center) NO.66320 The strain is obtained by being separated from soil and domesticated, the zinc tolerance concentration of the strain can reach 2g / L, and the strain can efficiently convert inorganic zinc into organic active zinc which can be easily absorbed and utilized by a human body. The organic zinc prepared from the strain has a remarkable ABTS free radical scavenging capability, and also has tyrosine kinase and 5-alpha reductase inhibition capability; the organic zinc prepared from the talaromyces purpurogenum D2 can be used for preparing zinc-supplementing health-care products, can also be used as cosmetic active matter raw materials with free radical scavenging and whitening effects, and has a wide market application prospect.