93 results about "Malignant transformation" patented technology

Provided is an HPV E6, E7 mRNA assay, referenced herein as the “In Cell HPV Assay,” that is capable of sensitive and specific detection of normal cervical cells undergoing malignant transformation as well as abnormal cervical cells with pre-malignant or malignant lesions. The In Cell HPV Assay identifies HPV E6, E7 mRNA via in situ hybridization with oligonucleotides specific for HPV E6, E7 mRNA and quantitates the HPV E6, E7 mRNA via flow cytometry. The In Cell HPV Assay can be carried out in less than three hours directly from liquid-based cervical (“LBC”) cytology specimens. The In Cell HPV Assay provides an efficient and highly sensitive alternative to the Pap smear for determining abnormal cervical cytology.

The invention discloses a preparation method of dendrobium officinale caulis wine. Bacillus coagulans and saccharomycetes are mixed and fermented, wherein the bacillus coagulans is an aerobic bacterium; in a fermentation process, the mildewing of dendrobium officinale caulis and the malignant transformation of flavor substances can be inhibited by consuming oxygen; an experiment verifies that an anti-oxidization substance has good stability in the fermentation process and a storage process; the reservation of original nutrient components is facilitated, and a sufficient C source and N source exist in a fermentation system; the alcohol content of the prepared dendrobium officinale caulis wine is 6 volume percent to 8 volume percent; the prepared dendrobium officinale caulis wine has a coordinated taste, good color and luster and an excellent flavor, and has the characteristics of abundant nutrients, health-care function and the like; an operation process is relatively simple and the wine is not easily infected by infectious microbes; only one time of fermentation is performed and a fermentation period is short; a fermentation byproduct can be used as a raw material of stuffing or is dried by mist spraying to obtain dendrobium officinale caulis powder; the loss of the nutrient components of the obtained product is less and the product does not easily go moldy in a processing process; the product has good quality and an excellent mouth feel, good comprehensive utilization is realized, and the dendrobium officinale caulis wine is suitable for being produced in a factory in a large batch.

An object of the present invention is to identify genes exhibiting characteristic behavior in the cases of carcinoma such as oral squamous-cell carcinoma using a change of expression level of micro RNA in oral squamous-cell carcinoma as an indicator, so as to provide a method for detecting carcinoma and an agent for suppressing carcinoma. The present invention A method for detecting oral squamous-cell carcinoma, which comprises detecting malignant transformation of specimens by employing a change of expression level of micro RNA as an indicator, and an agent for suppressing oral squamous-cell carcinoma using micro RNA.

Many GTPases such as Ras, Ral and Rho require post-translational farnestylation or geranylgeranylation for mediating malignant transformation. Dual farnesyltransferase (FT) (FTI) and geranylgeranyltransferase-I (GGT-1) inhibitors (GGTI) were developed as anticancer agents from based on an ethylenediamine scaffold. On the basis of a 4-fold substituted ethylenediamine scaffold, the inhibitors are structurally simple and readily derivatized, facilitating extensive structure-activity relationship studies. The most potent inhibitor is compound exhibited an in vitro hFTase IC50 value of 25 nM and a whole cell H-Ras processing IC50 value of 90 nM. Several of the inhibitors proved highly selective for hFTase over the related prenyltransferase enzyme geranylgeranyltransferase-I (GGTase-I). A crystal structure of an inhibitor cocrystallized with farnesyl pyrophosphate in the active site of rat FTase illustrates that the para-benzonitrile moiety is stabilized by a π-π stacking interaction with the Y361β residue, suggesting an importance of this component of the inhibitors.

The invention discloses a chrysotile-induced human pleural mesothelial cell malignant transformation strain and an application thereof. The human pleural mesothelial cell malignant transformation strain is classified and named as human pleural mesothelial cells, the strain number is AS-T, and the preservation number is CCTCC NO.C201923. The human pleural mesothelial cell malignant transformation strain is obtained by carrying out malignant transformation on human pleural mesothelial cells (MeT-5A) after being subjected to chrysotile multi-stage multi-time contamination treatment, and has a good application prospect in multiple aspects such as a cell model for researching a carcinogenic mechanism of carcinogenic substances and the like.

It is an objective of the present invention to provide a highly stable and reproducible method for detecting gastric cancer, by finding a novel gastric cancer-related gene and detecting the inactivation of such cancer-related gene. The present invention provides a method for detecting gastric cancer, comprising detecting malignant transformation of a gastric tissue cell by detecting the inactivation of the human VLDLR gene in the gastric tissue cell.

The invention provides a gRNA sequence of a targeted editing bcr-abl fusion gene and an application thereof. By location of three specific target sequence site DNA fragments in a bcr-abl gene, a gRNAexpression plasmid is constructed for a specific site, the plasmid and a FokI-dCas9 expression plasmid and a donor nuclear are transfected into a K562 cell, so that the bcr-abl gene is subjected to fixed-point fracture, homologous repair is performed by using a donor DNA as a template, and 8 basic groups are inserted to ultimately lead to disruption of the bcr-abl gene. Significant advantages of the sequence and the application thereof are that RFNs constructed for the pecific target sequence site in a bcr-abl sequence can efficiently target the bcr-abl gene to cause occurrence of DNA double-strand break, repair is performed in a HDR manner to enable bcr-abl to undergo mutation such as frame shift and to be destroyed, thereby losing potential for malignant transformation such as proliferation and apoptosis suppression.

The present invention provides compositions and methods for detecting MMP-induced malignancies by detecting Rac1b expression. The invention further provides compositions and in vitro and in vivo methods for inhibiting MMP-induced malignant transformation by modulating Rac1b expression and / or function.

The invention relates to a method for inducing a cell malignant transformation culture model. The method comprises the following steps: (1) culturing immortalized human gastric mucosal epithelial GES-1 in a DMEM culture solution containing 10% fetal bovine serum to obtain cultured cells; (2) culturing Cag A-positive helicobacter pylori J99 in a brain heart infusion medium containing 10% of newborncalf serum and centrifuging to remove a supernatant to obtain cultured bacteria; (3) diluting the cultured bacteria with DMEM, adding the bacteria into the cultured cells for induction by inoculationevery other day, adding an MNNG solution daily, and obtaining an induced helicobacter pylori infection model 48 weeks later. The method is simple and convenient, not only various indexes of malignanttransformation of the cells can be observed, but also the cell model in which the helicobacter pylori and MNNG work together for long-term for malignant transformation of gastric mucosa can be established, and a good experiment foundation is laid for study of the subsequent gastric cancer formation mechanism and the development of cancer treatment drugs.