Scid mouse model of electromagnetic radiation carcinogenesis and constructing method thereof

A technology of electromagnetic radiation and mouse models, applied in biochemical equipment and methods, electric/wave energy treatment of enzymes, treatment of microorganisms with electric/wave energy, etc., to achieve simple steps, significant effects, and prevent long-term harm

Inactive Publication Date: 2010-09-22
BEIJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the method of using Scid mice to construct an animal model of electromag...

Method used

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  • Scid mouse model of electromagnetic radiation carcinogenesis and constructing method thereof
  • Scid mouse model of electromagnetic radiation carcinogenesis and constructing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Culture and passage of NIH / 3T3 cells

[0025] NIH / 3T3 cells were incubated at 37°C, 5% CO 2 Culture in the incubator with DMEM culture medium. When the cells grow and proliferate to form a single layer of cells and expand to confluence, suck out the old culture medium, add 0.03% Versene solution to wash once, suck out the washing liquid, and then add trypsin digestion solution for digestion at 37°C. At the same time, observe the digested cells under an inverted microscope. When the original adherent cells gradually tend to be round, the cytoplasm retracts, the gap increases, and the cells are no longer connected into sheets, stop the digestion immediately, discard the digestion solution and add fresh Use a pipette to blow the digested cells into a single-cell suspension, aspirate the cell suspension and divide it into 3 culture bottles, add the culture medium and tighten the bottle cap, put in CO 2 Continue culturing in the incubator;

[0026] (2) Exposure experim...

Embodiment 2

[0034] The power density of electromagnetic radiation is 50W / m in the step (2) 2 , other steps are with embodiment 1. Both Scid mice developed tumors.

Embodiment 3

[0036] The power density of electromagnetic radiation in the step (2) is 70W / m 2 , other steps are with embodiment 1. Both Scid mice developed tumors.

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Abstract

The invention relates to a Scid mouse model of electromagnetic radiation carcinogenesis and a constructing method thereof. The method comprises the following steps of: culturing an NIH/3T3 cell and carrying out passage; exposing the cell in the presence of the electromagnetic radiation of 30-90W/m<2> and culturing for 4-6 weeks; and carrying out a soft agarose culturing experiment and inoculating the cultured soft agaroes into a Scid mouse for carrying out an oncogeen experiment. The result shows that a cell colony is formed in the soft agarose experiment and Scid mice in the oncogeen experiments all generate tumors. By using the method, the Scid mouse model with tumors induced by using the electromagnetic radiation can be established; and the method has the advantages of remarkable effect, simple steps and favorable repeatability. An in vitro cell transformation experimental model established by the method can be used for simulating the vicious transformation of an in vivo cell and provides a favorable experimental model and a powerful technical means for the research on a pathogenesis and prevention and treatment measures of the tumor induced by the electromagnetic radiation. The method can be applied to the carcinogenic risk pre-evaluation on the electromagnetic radiation in the industry, science, medical treatment and daily life and has an important value of preventing the long-term harm of the electromagnetic radiation on the human health.

Description

technical field [0001] The invention relates to a Scid mouse model and a construction method of electromagnetic radiation-induced cancer. Background technique [0002] With the rapid development of science and technology, electromagnetic technology has become one of the indispensable and important tools in the fields of industry, medical treatment and communication. While electromagnetic technology is convenient for people's daily life, more and more attention has been paid to its influence on the human body. Electromagnetic waves can penetrate into living organisms and interact with biological tissues, leading to changes in morphology, structure and function at different biological levels, and even inducing cancer. How to prevent or reduce the harm of electromagnetic radiation to the human body has increasingly become a research hotspot in the fields of life science, environmental science and medical health. Through in-depth research on the biological effects of electroma...

Claims

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Application Information

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IPC IPC(8): C12N5/073C12N13/00A01K67/027
Inventor 曾毅赵丽娇崔亚松钟儒刚
Owner BEIJING UNIV OF TECH
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