1381 results about "Digestion" patented technology

The invention relates to selenium-rich health-care rice dumplings. The selenium-rich health-care rice dumplings are prepared from the following raw materials of selenium-rich glutinous rice, rose petals, haw tree parasitism, plum tree parasitism, radix rehmanniae, dendrobium candidum, vegetable oil, pork, mung beans, ground cinnamon and table salt. The selenium-rich glutinous rice is used as a main raw material, so that selenium elements in human bodies are complemented, and collective antioxidation is strengthened; besides, the glutinous rice and the mung beans are soaked in a traditional Chinese medicine extracting solution, and medical components in traditional Chinese medicines are absorbed, so that the selenium-rich health-care rice dumplings have the efficacies of clearing heat, promoting salivation, relieving restlessness, resolving stagnation, reducing blood lipid, promoting digestion and enhancing the immunity of organisms.

The invention provides a whey protein deep hydrolysate with low sensitization and an allergy relieving effect as well as a preparation method and application of the whey protein deep hydrolysate. According to the method, the alkaline protease, the neutral protease and the glutamyltransferase are sequentially used for carrying out first enzymolysis, second enzymolysis and third enzymolysis on the whey protein raw material, and the whey protein hydrolysate which is low in molecular weight, good in taste, good in digestion and absorption performance, low in sensitization and capable of effectively relieving allergic reaction of the organism is obtained.

The invention relates to the technical field of microorganisms, in particular to bifidobacterium bifidum BGLP1 capable of regulating sugar digestion and producing GLP-1 at high yield and application of the bifidobacterium bifidum BGLP1. The preservation number of the bifidobacterium bifidum BGLP1 is CGMCC (China General Microbiological Culture Collection Center) No.35718, and the bifidobacterium bifidum BGLP1 can be used for preparing the feed additive. The strain has the capability of inhibiting alpha-glucosidase and alpha-amylase; sTC-1 cells are stimulated to secrete GLP-1, and the blood glucose reducing pathway capacity of the intestinal tract is activated; the activity of pancreatic lipase is obviously inhibited, and the dietary fat absorption capability can be effectively reduced; redundant cane sugar is converted into exopolysaccharide, so that the probiotic function capability is improved; the capability of converting white fat into brown fat is promoted; macrophages are activated, and the body immune function is improved. In addition, the bifidobacterium bifidum BGLP1 also has relatively strong intestinal colonization ability, has good tolerance in artificial gastric juice and artificial intestinal juice, and can smoothly reach the intestinal tract of a human body.

The invention provides infant rapid brewing rice flour based on enzymolysis and superfine water grinding and a preparation method thereof, and belongs to the technical field of food processing. Rice is subjected to raw material differential temperature pretreatment and multi-stage hydration pulping, and a stable three-dimensional microscopic network structure with starch particles as filler and soluble protein and colloid as a continuous phase is constructed; then, food-grade lactic acid bacteria pre-fermentation is combined with sequential action of various enzymes, and starch and protein are subjected to mild directional hydrolysis under the condition of simulating dynamic pH of gastrointestinal tract digestion, so that the nutritional and digestible infant healthy rice flour is prepared, and the problems that the rice flour is easy to agglomerate, non-uniform in dispersion and low in nutritional value during brewing are solved. The digestion and absorption efficiency of macromolecular nutrient components such as starch and protein is relatively low, and the nutrition and flavor of the product mainly depend on exogenous addition, so that the naturalness and functionality are insufficient. The rice flour disclosed by the invention has excellent reconstituability and nutrient absorption rate, and is suitable for being used as a healthy nutritional complementary food for infants of 6-36 months.

The invention provides a water-blocking microcapsule, a fish oil composite hair beautifying ointment and a preparation method, and relates to the technical field of animal health products. The water-blocking microcapsule is a solid particle formed by a core material and a wall material coating the core material; the core material is fish oil emulsion subjected to bile salt emulsification treatment; the wall material sequentially comprises an inner-layer wall material and an outer-layer wall material from inside to outside; the inner-layer wall material is a wall material formed by solidifying alginate; and the outer-layer wall material is a water-blocking wall material which is arranged outside the inner-layer wall material and is made of nanocrystalline cellulose. The water-blocking microcapsule provided by the invention realizes multiple beneficial effects of efficient digestion and absorption of fish oil, improvement of palatability, enhancement of oxidation resistance and stability, fixed-point release of intestinal tracts and the like, provides an innovative technical scheme for development of animal health products, and has wide application prospects and market values.

The invention discloses modified biogas residue-based hydrothermal carbon, a preparation method thereof and a dry digestion method for kitchen waste, and belongs to the technical field of waste organic matter recycling. The preparation method comprises the following steps: soaking biogas residues in a phosphoric acid solution to obtain pretreated biogas residues; mixing the pretreated biogas residues, transition metal salt, biochar loaded with vitamin B12 and water with the mass being 8-15 times that of the pretreated biogas residues, and performing hydrothermal reaction for 1-4 hours under the conditions that the temperature is 180-250 DEG C and the pressure is 1.5-4 MPa to obtain a hydrothermal product; and activating the hydrothermal product for 1-2 hours under the protective atmosphere of 300-400 DEG C to obtain the modified biogas residue-based hydrothermal carbon. The surface Fe-Cu composite oxide sequentially catalyzes and oxidizes H2S into elemental sulfur and sulfate ions, the vitamin B12-loaded biochar can promote MA directional proliferation, the sulfate ions can competitively inhibit SRB substrate uptake, the quantity ratio of MA to SRB is increased, and the methane production efficiency is effectively improved.

The invention discloses a strain of lactobacillus fermentum LF-HF25 obtained by screening sour cucumber, which is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC No.M20241566, the classification name is the lactobacillus fermentum LF-HF25, the preservation date is July 15, 2024, and the preservation address is Wuhan University, China. The lactobacillus fermentum LF-HF25 has the advantages that the preservation number is CCTCC No.M20241566, the classification name is the lactobacillus fermentum LF-HF25, the preservation date is July 15, 2024, and the preservation address is Wuhan University, China. The lactobacillus mucilaginosus LF-HF25 or the metabiotic microbial inoculum prepared from the lactobacillus mucilaginosus LF-HF25 has the functions of dispelling the effects of alcohol, protecting the liver, promoting digestion and nourishing the stomach, also has the effect of improving sleep, and has a wide application prospect.

The application provides an extendable digestive tract cannula and application thereof, and relates to the technical field of medical devices. The application comprises a first cannula, a second cannula and a degradable material. Opposite ends of the first cannula are a proximal end and a distal end, respectively. The first cannula is sleeved with the second cannula, and a double-layer structure is formed at the distal end of the first cannula. The double-layer structure is fixed by the degradable material. When the degradable material degrades, the second cannula moves towards the end face of the distal end of the first cannula. By setting the movable second cannula, the second cannula moves backward after the degradable material degrades, so that the length of the digestive tract cannula is extended. Since the length of the digestive tract cannula is increased, the area of intestinal absorption is also increased, the compensatory absorption function of the intestine at the distal end of the digestive tract cannula is weakened, the isolation effect of chyme and the intestine is improved, and the digestion and absorption effect of the intestine can be reduced for a long time.

The invention relates to the technical field of biology, in particular to a method and application of fungus-enzyme synergistic fermentation of bagasse pith, and the method comprises the following steps: (1) adjusting the water content of the bagasse pith to 60-70%, and adjusting the pH to 4.0; (2) adding the following components according to dry matter mass: 3% of phanerochaete chrysosporium liquid with the concentration of 1.5 * 10 < 8 > CFU / ml, and uniformly mixing; 1% of sucrose; the cellulase liquid is 20000U / kg, and the cellulase liquid is prepared by dissolving crude enzyme obtained by centrifuging after trichoderma hedgehog is fermented to produce enzyme in a PBS (phosphate buffer solution); and (3) putting the mixture into a fermentation tank, and performing sealed low-oxygen fermentation for 30 days to obtain the bagasse pith feed. According to the bagasse pith feed obtained by the method, the nutritional value of the bagasse pith can be improved, the fermentation quality is improved, the digestion potential is improved, the storage time is prolonged, and high-quality feed application of the bagasse is realized.

The present application belongs to the technical field of medical care, and particularly relates to a nutrient solution delivery device for assisting in nursing of esophageal cancer patients, which comprises a mounting seat, the inside of the mounting seat is provided with a fixing part, the fixing part can extrude and fix the nutrient bag placed inside to avoid the nutrient bag from deviating during the operation of the equipment; the inside of the fixing part is provided with a driving part, the driving part moves back and forth inside the mounting seat, the driving part can extrude the nutrient solution inside the nutrient bag to flow during the back and forth movement; the inside of the driving part is further provided with a stirring part which rotates, the stirring part can rotate by using the power of the back and forth movement of the driving part, and the rotation of the stirring part further promotes the flow of the nutrient solution inside the nutrient bag; the inside of the mounting seat is provided with a delivery part. The present application uniformly heats the nutrient solution and delivers it into the gastrointestinal tract of the patient, the warm nutrient solution can promote blood circulation and increase the peristalsis of the intestinal tract, which is helpful to improve the digestion and absorption rate of the nutrient components.

The invention discloses a composition for regulating body weight through satiety-sugar control double channels. The composition is prepared from the following components in parts by weight: 5-25 parts of cinnamon extract, 1-20 parts of ganoderma lucidum EGCG composite powder, 10-40 parts of L-arabinose, 5-40 parts of composite fruit and vegetable fiber powder, 5-25 parts of fructus cannabis protein peptide, 1-20 parts of apple polyphenol, 1-20 parts of white kidney bean dietary fiber powder and 1-20 parts of agaricus bisporus powder. The composition is characterized in that digestion and absorption of carbohydrate are inhibited, the swelling ratio larger than or equal to 35 times is achieved through a composite fiber system, gastric emptying is remarkably prolonged, satiety hormones such as GLP-1 are adjusted, and the composition can be used as a non-alternative weight management product and is good in safety.

The invention belongs to the technical field of chemical detection, and particularly relates to a method for detecting the content of boron in soil and sediment, which comprises the following detection methods: S1, weighing a sample and putting the sample into a digestion tank; s2, adding a digestion solution into the digestion tank; s3, after uniform stirring, putting the digestion tank into a microwave digestion instrument for microwave digestion; s4, after digestion is completed, closing the microwave digestion instrument, and after the digestion tank is cooled to the room temperature, filtering to remove precipitates; s5, transferring the digestion solution into a volumetric flask, and fixing the volume for subsequent analysis; and S6, analyzing the digestion solution by using ICP-OES / MS (Inductively Coupled Plasma-Optical Emission According to the method for detecting the boron content in the soil and the sediment, ammonium fluoride is used for replacing hydrofluoric acid in boron detection of the soil and the sediment, use of toxic reagents is reduced, mannitol is added, a stable complex is formed by mannitol and boron, and the recovery rate of boron is increased.

The invention belongs to the technical field of food processing, and relates to a method for preparing low-salt shrimp juice through rapid microbial fermentation. The method comprises the following steps: removing heads of shrimps, preparing meat paste, drying and crushing shrimp shells, and mixing the shrimp shells with the meat paste; carrying out enzymolysis treatment on the meat paste by using alkaline protease, and then carrying out high-temperature enzyme deactivation; mixing the shrimp meat and shrimp shell enzymatic hydrolysate mixture with soybean meal and bran, and sterilizing at high temperature; inoculating a complex microbial inoculant containing lactobacillus plantarum, saccharomyces rouxii, aspergillus oryzae, deep-sea bacillus subtilis and bacillus subtilis to prepare a mold starter, fermenting at 28 DEG C for 30 days, and finally filtering and sterilizing. According to the method disclosed by the invention, the availability of the raw materials is effectively improved, the fermentation period is shortened, efficient fermentation under a low-salt condition is realized, various basic indexes of the obtained shrimp juice product meet national standards, and the shrimp juice product is outstanding in flavor quality and has rich sauce flavor and sweet taste. In addition, the shrimp juice shows good antioxidant stability before and after digestion.

The invention provides a method for overspeed preparation of gene edited goldfish. The method comprises the following steps: S1, obtaining gobiocypris rarus without primordial germ cells as receptor fish; s2, designing a target spot for the goldfish amh gene, introducing a Cas9 / gRNA complex into a goldfish fertilized egg, performing sequencing verification to obtain a mutant individual, and feeding the mutant individual as a donor fish; s3, taking gonads of donor fish, cutting into pieces, performing enzymolysis digestion, filtering, and performing density gradient centrifugation to enrich donor germline stem cells; s4, transplanting the germline stem cells of the donor fish into the receptor fish body, and feeding to obtain the gene edited goldfish sperms and ova. S5, hybridizing and feeding goldfish amh mutant sperms and ova produced by gobiocypris rarus to obtain gene edited goldfish individuals. According to the invention, gobiocypris gobiocypris is used as a receptor, the cross-subfamily gene edited goldfish is used as a donor, and an efficient, stable and easy-to-popularize method for preparing the gene edited goldfish is provided by utilizing a belly borrowing reproduction technology.

The invention discloses a method for preparing calcium hydroxide with a high specific surface area by digesting calcium oxide, and relates to the technical field of preparation of calcium hydroxide with a high specific surface area. The method is characterized in that diethylene glycol / triethanolamine / magnesium chloride is adopted as a compound digestion aid through two processes of wet digestion and dry digestion; and calcium hydroxide with high specific surface area can be controllably prepared. The specific surface area of the calcium hydroxide prepared through wet digestion can reach 70 m < 2 >. G <-1 > or above, the specific surface area of the calcium hydroxide prepared through dry digestion is 45 m < 2 >. G <-1 > or above, the reaction activity of the calcium hydroxide is effectively improved, and the novel controllable method for preparing the calcium hydroxide with the high specific surface area is provided.

The invention discloses a single-cell probiotic packaging method of thiolated guluronic acid oligosaccharide and a probiotic microcapsule, and relates to the technical field of biological medicines. The packaging method comprises the following steps: mixing a thiolated guluronic acid oligosaccharide solution with a probiotic suspension to form a probiotic-polysaccharide mixed solution; and adding a calcium chloride solution into the probiotic-polysaccharide mixed solution, and stirring to form the single-cell microcapsule encapsulating the probiotics. The packaging method can protect a single strain from being damaged by an external adverse environment to the maximum extent, and the survival rate is increased. Meanwhile, the obtained single-cell microcapsule can improve the digestion of the probiotics, the survival rate of the probiotics under the oxidative stress environment and the antibiotic combination, has high stability, and prolongs the intestinal residence time of the probiotics. An innovative solution is provided for the problems that in the existing medicine field, probiotic preparations cannot improve the gastrointestinal stress resistance of probiotics, and antibiotics lose efficacy when combined.

The invention discloses lactobacillus plantarum OBM7 and application thereof, and belongs to the technical field of biology. The strain is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on November 12, 2025, the preservation address is the 5th floor of the building 59, No.100 Courtyard, Xianlie Middle Road, Guangzhou, and the preservation number is GDMCC NO. 67282; and the probiotic preparation does not contain antibiotics or drug residues, and meets the development requirements of green and healthy breeding. The probiotic preparation can specifically prevent and control lamb diarrhea, significantly reduce the fatality rate of lamb diarrhea, effectively improve the survival rate of lambs, and provide targeted protection for lamb intestinal health; the lamb feed can regulate lamb gastrointestinal tract micro-ecological balance, improve digestion and absorption functions, significantly improve feed intake and feed conversion efficiency, and promote lamb growth performance improvement. Reliable technical support is provided for efficient, green and healthy breeding of lambs, high practicability and wide industrial application prospects are achieved, and the method has great significance in promoting green transformation of the livestock breeding industry.

The invention provides a method for eliminating ribosomal RNA in a mouse total RNA sample, which comprises the following steps: carrying out reverse transcription on the total RNA in the mouse sample into cDNA, and amplifying the cDNA by adopting a primer sequence group to obtain a DNA fragment covering all sequences of the mouse ribosomal RNA; carrying out amplification by taking the DNA fragment as a template and adopting a single primer in the primer sequence group to obtain a single-stranded DNA probe; hybridizing the total RNA of the mouse sample by adopting a single-stranded DNA probe, and performing RNase H digestion to obtain a digestion product; sequentially carrying out DNase I digestion and RNA purification on the digestion product to obtain a target product; wherein the primer sequence group comprises upstream primers from the first to the nineteenth and downstream primers from the first to the nineteenth, and the primer sequence group has the characteristics of low cost, stable performance and high reduction efficiency.

The invention belongs to the technical field of intelligent animal husbandry and biosensing, and discloses a health assessment system and method based on cattle rumination voiceprint recognition, and the system comprises an intelligent necklace terminal, an edge calculation unit and a cloud health assessment engine. The intelligent necklace integrates voiceprint collection, jaw movement perception and environment compensation units, and obtains ruminant voiceprint, jaw movement and environment data of cattle in real time; the edge calculation unit optimizes voiceprint signals through a dynamic noise suppression module in combination with environmental parameters, and synchronously analyzes voiceprint and motion features by using a feature joint extraction module; and the cloud end adopts a lightweight hybrid model to perform state classification on ruminant voiceprints, fuses motion features and environmental data to generate health indexes, and triggers early warning or links a feed library to generate an accurate feeding scheme. The cattle digestion health real-time monitoring system realizes cattle digestion health real-time monitoring through non-intrusive voiceprint sensing and multi-modal data analysis, and is especially suitable for pasture accurate management, disease early warning and feeding strategy optimization.

The invention belongs to the technical field of traditional Chinese medicine tablet manufacturing, and particularly relates to an online quality prediction method for stomach invigorating and digestion promoting tablets based on multi-modal fusion. Comprising the following steps: synchronously acquiring hyperspectral imaging data and near infrared spectrum data of a stomach invigorating and digestion promoting tablet sample; processing the near infrared spectrum data through a one-dimensional convolutional neural network to extract a first spectrum feature; parallel extraction of spatial features and spectral features is carried out on the hyperspectral imaging data through a double-branch feature extraction network; performing intra-modal fusion on the extracted spatial features and the second spectral features through a cross attention mechanism to obtain hyperspectral fusion features, and performing cross-modal fusion on the hyperspectral fusion features and the first spectrum through cross-modal multi-head attention to generate fusion features; and outputting a quality index prediction value of the stomach invigorating and digestion promoting tablets through a regression prediction module based on the fusion features.

The invention provides a lysosome extraction method. The lysosome extraction method comprises the following steps: (1) extracting cells; (2) tissue extraction; (3) crude separation of lysosome; and (4) separating the lysosome by magnetic beads. Through EDTA-free digestion optimization, lysis solution and buffer solution formula optimization, a filter membrane interception-magnetic bead purification combined technology, a double-antibody synergistic capture strategy and a competitive mild elution system, the four core problems of low purity, large activity damage, tedious operation and strong equipment dependence in a traditional lysosome separation technology are systematically solved. Experimental data show that the lysosome yield and functional integrity of the method are obviously superior to those of a density gradient centrifugation method, and the method is suitable for trace samples and conventional equipment and has high conversion value. In the future, the technical barrier can be further optimized through automation equipment integration and multi-dimensional function verification, and the application of the method in basic research and clinical diagnosis is promoted.

The invention relates to the technical field of circulating fluidized bed flue gas desulfurization, and discloses an automatic control system applied to a CFB desulfurization digester. The feeding unit is used for conveying quick lime and comprises a quick lime bin, a screw feeder and a belt conveying scale which are connected in sequence, and the belt conveying scale is in signal connection with the digestion automatic controller; the digestion reaction unit comprises a three-stage digester and a plurality of digestion thermometers arranged in the three-stage digester, and the digestion thermometers are in signal connection with the digestion automatic controller. The water-cement ratio is dynamically adjusted by monitoring the temperature change trend in real time, fluctuation of the quality of quick lime can be automatically adapted, and the limitation of fixed water-cement ratio control is overcome; by searching the optimal water-cement ratio, the full digestion of the quick lime is ensured, and the activity and uniformity of the slaked lime product are improved; and moreover, the dependence on artificial experience is reduced through automatic control, and the stability and continuity of system operation are improved.

The invention discloses a preparation method of highland barley prefabricated powder with a low glycemic index and application of the highland barley prefabricated powder in 3D printed biscuits. According to the method, firstly, cellulase and alpha-amylase are adopted for carrying out enzymatic modification treatment on highland barley flour, the content of anti-digestion starch (RS) is remarkably increased, the content of fast-digestion starch (RDS) is reduced, and therefore the modified highland barley flour with the predicted glycemic index (pGI) reaching the low-GI food standard (GI is smaller than or equal to 55) is prepared, and the dietary requirements of people with different metabolic requirements are met. Further, the modified highland barley powder is compounded with sodium alginate, glutamine transaminase is utilized for inducing and constructing a protein-polysaccharide gel network, the incubated and cross-linked dough is subjected to freeze drying and smashing, and the highland barley prefabricated powder with the low glycemic index is obtained.The 3D printing adaptability, the structural stability and the digestion resistance of the material are effectively enhanced through the method. The highland barley biscuit has the characteristic of low glycemic index, and is suitable for making healthy food for special crowds with diabetes and the like.

The invention discloses a digestible quality analysis system for chicken feed, relates to the technical field of chicken feed analysis, and aims to solve the problem of poor simulation effect of digestion of the chicken feed. According to the method, low-temperature and high-temperature staged drying treatment is adopted, the characteristics of components such as moisture and fat in the residues are fully considered, the secretion rules of different alimentary canal parts of animals are simulated, sufficient and reasonable contact of digestive juice and feed is ensured, the simulation process is closer to the real physiological condition, and the situation that the digestion analysis result is affected by liquid amount errors is avoided; animal digestive tracts are scientifically segmented into stomach, small intestine and large intestine stages, corresponding external buffer solution and internal digestive juice are injected respectively, and a real digestion scene is comprehensively simulated from a physical structure to a chemical environment.

The invention discloses a firework and cracker hazardous waste recovery treatment method and system, and the method comprises the following steps: a wastewater treatment step: the wastewater treatment step comprises a chemical precipitation step, an A2O biochemical treatment step, a Fenton oxidation step and a BAF biological filter step, and perchlorate, COD and heavy metal are removed; the waste residue treatment step comprises a wet grinding step, a high-temperature digestion step, a flotation step, a hydraulic cyclone separation step, a wet leaching step and a fractional precipitation step, and carbon powder, titanium, barium, aluminum and magnesium resources are recycled; according to a synergistic coupling mechanism, bottom slag generated in the wastewater treatment step is conveyed to the waste slag treatment step for treatment, and filtrate generated in the waste slag treatment step is conveyed to the wastewater treatment step for treatment. The method is used for carrying out harmless treatment and resource recycling on the hazardous wastes of the fireworks and crackers.

The invention discloses a lysozyme-xanthan gum conjugate nano-particle for improving bioavailability of pleurotus eryngii immune active peptide, which is a nano-particle loaded with pleurotus eryngii immune active peptide prepared by taking lysozyme, xanthan gum and pleurotus eryngii immune active peptide as raw materials through a Maillard reaction controllable damp-heat method. Wherein the amino acid sequence of the pleurotus eryngii immune active peptide is LLGVD. By combining the mild and controllable Maillard reaction with the ultrafiltration purification technology, high-proportion and high-activity loading of the pleurotus eryngii immunocompetent peptide is realized under the mild condition, and the structural integrity and immunomodulatory activity of the pleurotus eryngii immunocompetent peptide are effectively maintained; wide application prospects are realized in the aspect of developing functional foods or related products for improving low immunity. The physical stability, the digestion resistance and the potential oral bioavailability of the active peptide are remarkably improved, and the whole process is safe, green and relatively simple and has good amplification potential.

The invention provides a method for detecting the content of elements in carbonized resin. The method comprises the steps of resin pretreatment, instrument tuning, standard curve drawing, sample detection and the like. The method comprises the following steps: firstly, transferring a certain amount of dry carbonized resin into a cleaned PFA digestion tank, adding high-purity mixed acid, setting a segmented heating program to start microwave digestion, cooling to room temperature after digestion is completed, adding a proper amount of ultrapure water to dilute a digestion solution, and adding an internal standard solution and a chemical additive to obtain a to-be-detected sample solution; preparing a multi-element mixed standard solution according to the sample element types, adding the standard solution into the sample solution, and measuring the signal intensity ratio R of the to-be-measured element to the internal standard in each solution; and drawing a standard curve by taking the labeling concentration as a horizontal coordinate and R as a vertical coordinate, correcting the standard curve, and detecting the sample to be detected. The method is used for detecting the content of elements in carbonized resin, the carbonized resin is digested through microwaves, and the obtained solution is clear and can be directly subjected to ICP-OES (Inductively Coupled Plasma-Optical Emission Spectrometer) detection.

The invention particularly relates to a pet food containing Chinese herbal medicines and capable of maintaining intestinal health of pets and a preparation method of the pet food. The pet food comprises an inner-layer structure and an outer-layer structure covering the surface of the inner-layer structure, wherein the inner-layer structure is a ball intermediate prepared by mixing Chinese herbal medicine powder, a nutritional additive and a binder; the outer layer structure comprises a meat source; the binder comprises a cartilage hydrolysate; the Chinese herbal medicine powder in the inner layer of the pet food is Chinese herbal medicine powder for improving gastrointestinal functions; the nutritional additive comprises inactivated probiotics, prebiotics, compound digestive enzymes, chicken liver hydrolyzed powder and auxiliary materials; by optimizing the formula and the process, the palatability of the pet food is ensured, the digestion and absorption conditions of pets are improved, the intestinal problems can be effectively prevented and treated, nutrition and drug therapy are integrated, and the overall health of the pets is promoted; good commercial application prospects are realized.

The present invention discloses a liquid nutritional meal replacement and fat-reducing food and a preparation method thereof, comprising the following steps: S1, preparation of a composite resistant dextrin; S2, preparation of a Poria cocos extract; S3, preparation of a composite plant extract; S4, preparation of the liquid nutritional meal replacement and fat-reducing food. The liquid nutritional meal replacement and fat-reducing food has high nutritional value, good taste, and a strong sense of fullness. By preparing resistant dextrin using potato starch as a raw material, then coating it with corn starch, and supplementing it with a composite plant extract, it can not only improve the sense of fullness, but also slow down the digestion rate of the meal replacement food in the stomach and intestines, thereby reducing the rapid increase in blood sugar after a meal, having a significant effect on weight control, and slowing down the gas production rate of the resistant dextrin fermented in the intestines, reducing the discomfort caused by abdominal distension.

The invention provides a construction method, system and product of a false positive judgment model of a mutation site obtained by sequencing after enzyme digestion fragmentation and related application. Specifically, the false positive judgment model is constructed on the basis of a plurality of characteristic parameters associated with mutation sites obtained by enzyme digestion fragmentation sequencing; the characteristic parameters comprise scores obtained by performing reverse complementary sequence alignment on the front and back extension 30bp of the mutation site, the sequence proportion of 15 basic groups at the tail end of the mutation site, the Bayesian conditional probability of the extracted mutation site, and the KS test statistic of the extracted mutation site. Furthermore, the invention also provides a method for constructing the model by utilizing the crowd detection rate and the corresponding false positive. According to the method, the data characteristics of the false positive mutation sites are introduced by utilizing digestion fragmentation, so that the reliability of the digestion false positive mutation sites is objectively evaluated under the condition that high depth and large data volume are not needed, and the detection accuracy of the mutation sites can be remarkably improved.