Belonging to the technical field of fruit vinegar, the invention relates to a lonicera japonica fruit vinegar and a making method thereof. The lonicera japonica fruit vinegar is composed of lonicera japonica, grape, hawthorn, kiwi fruit, pomegranate, Chinese wolfberry, lemon, pineapple, white granulated sugar, active dry yeast, honey, purified water and an acetobacter culture solution. Because of lonicera japonica and other materials, the lonicera japonica fruit vinegar provided by the invention can provide various vitamins, amino acids and the like needed by the human body, also can provide various organic acids promoting human body circulation, is suitable for long-term moderate drinking, and can improve the human body immunity and eliminate human fatigue. Also, the main raw materials have wide sources, and the production process is simple and is easy to popularize.