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A method for detecting human fatigue using saliva

A technology for saliva detection and human body, which is applied in the field of protein detection, can solve the problems of non-invasiveness and not yet established, and achieve the effect of easy measurement, easy availability and easy detection

Active Publication Date: 2019-01-18
HEBEI UNIV OF ENG
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  • Abstract
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  • Application Information

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Problems solved by technology

However, a non-invasive and convenient extraction and detection technology for proteins in saliva has not been established so far

Method used

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  • A method for detecting human fatigue using saliva
  • A method for detecting human fatigue using saliva
  • A method for detecting human fatigue using saliva

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Experimental program
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Embodiment

[0032] Fatigue is the root cause of inducing multiple diseases, and the present invention is further described in detail with the nucleonectin-2 content and human body fatigue state in measuring saliva below as an example, and concrete method is as follows:

[0033] A. Extraction and collection of saliva from suspected fatigued human body

[0034] First of all, before collecting saliva, generally rinse your mouth with normal saline three times, and then rinse your mouth with clear water three times. Each rinse with normal saline and clear water should not be less than twice. The volume of normal saline or clear water used for each rinse can be within Any value between 20-30ml is better if the mouth can move flexibly. The mouthwash time is about 25s each time, and any value between 20-30s can be taken. 20-35°C with comfortable taste, such as around 30°C. Then tilt the tongue up or perform chewing movements to make saliva accumulate in the lower jaw and contract through the low...

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Abstract

The invention discloses a method for detecting human body fatigue by utilizing saliva. The method comprises the following steps: extracting and collecting saliva; adding an adsorbent and a protease inhibitor into the collected saliva; and detecting the content of specific proteins in the saliva. The health condition is determined by detecting the content of the specific proteins in the saliva. The method disclosed by the invention belongs to the field of detection of human proteins, is a non-invasive marker detection method, does not damage human tissues, has the advantages of noninvasive and painless properties and rapidness, and has important social significance and application prospects.

Description

technical field [0001] The invention relates to a method for detecting human body fatigue by using saliva, and belongs to the technical field of protein detection. Background technique [0002] Protein accounts for about 18% of the total mass of the human body, and it is a substance closely related to life activity phenomena. Every cell and every major part of the body has proteins involved. There are many types of proteins in the human body, with different properties and functions, but they are all composed of more than 20 amino acids (Amino acid) in different proportions, and are constantly metabolized and renewed in the body. [0003] In the prior art, the extraction and inspection of proteins are mostly carried out by invasive extraction methods such as blood drawing. In 2008, Denny of the University of Southern California and others published an article in the "Journal of Proteomics Research" and pointed out that there are 1166 proteins in human saliva. Most of these...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/573
CPCG01N33/573G01N33/68G01N33/6893G01N2333/96425
Inventor 许岩丽刘志军赵朝贤李焰丁敏苏现辉刘凤丽熊南雁王雪玲刘小霞席爱萍
Owner HEBEI UNIV OF ENG
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