74 results about "Microinjection" patented technology

The invention discloses a zebra fish spermatogonial stem cell separation and transplantation method, and belongs to the technical field of fish germline stem cell separation. The method comprises the following steps: washing complete sexual glands of zebra fish testis by adopting penicillin / streptomycin double-antibody-containing PBS (Phosphate Buffer Solution), physically shearing, digesting and dissociating, and filtering by a screen, so as to obtain zebra fish testis cell suspension; performing density gradient centrifugation to obtain zebra fish spermatogonial stem cells; dyeing, marking, centrifuging, washing, re-suspending, and carrying out micro-injection transplantation to enter the back of the abdominal cavity of the acceptor zebrafish juvenile fish. According to different physical properties of different cells, spermatogonial stem cells are separated, purified and transplanted from zebra fish testis cell suspension by adopting a density gradient centrifugation method, and migration and proliferation processes of donor germ cells can be observed in receptor fish after fluorescence labeling. And a new technical path is provided for fish germplasm resource preservation, endangered fish protection, economic cultured fish yield increase and new variety cultivation.

The invention provides a grouper microinjection method, and belongs to the technical field of aquaculture. According to the method, grouper eggs are injected by utilizing a grouper microinjection needle, the microinjection needle is prepared by calcining a glass capillary tube by utilizing a needle pulling instrument, and the parameters of the needle pulling instrument are set as P = 500mW, HET = 720ms, PULL = 60, VEL = 20mu m / s and TIME = 250ms; the outer diameter of the glass capillary tube is 1.0 mm, the inner diameter of the glass capillary tube is 0.75 mm, and the length of the glass capillary tube is 10 cm. The method for injecting the grouper eggs with the micro-injection needle is simple and easy to operate, exogenous gene transfer and endogenous gene accurate editing of the grouper can be realized by adopting the method disclosed by the invention, the target gene editing efficiency is high, and powerful support is provided for deeply researching gene functions of the grouper and cultivating excellent culture varieties.

The invention provides a method for high-throughput rapid screening of food fermentation strains. The method comprises the following steps: mixing a to-be-detected bacterial sample with a culture medium without a yeast product, injecting an obtained mixed bacterial liquid and an oil phase into a liquid drop generation chip, generating liquid drops, and collecting the liquid drops for culture; injecting the cultured liquid drops, the oil phase and SYTOTM16 fluorescent dye liquid into a liquid drop micro-injection chip, collecting the generated liquid drops, injecting the liquid drops and the oil phase into a micro-fluidic sorting chip, carrying out fluorescence detection, collecting target liquid drops, and carrying out demulsification treatment and coating culture. The method can realize rapid and accurate high-throughput screening of target strains, and especially can directly quantify the growth activity of microorganisms under the stress of acid and salt, thereby realizing efficient and accurate screening of acid-resistant and salt-resistant strains. Moreover, the method does not need to carry out gene modification on the strain, avoids the problems of biological safety and technical complexity possibly caused by gene manipulation, retains the natural characteristics of the strain, is suitable for large-scale high-throughput bacterium screening, and has high application value.

The invention discloses an RNA (Ribonucleic Acid) interference method for parasitic wasps in lepidoptera larvae, which is characterized in that RNA interference is carried out by using a microinjection method or a nano-material immersion method when the parasitic wasps in the lepidoptera larvae are not separated from hosts or are separated from the hosts to cocoon, so that an RNAi system of the parasitic wasps is successfully constructed, and the defects that the parasitic wasps are small in size in an egg stage, and the parasitic wasps are not damaged in the egg stage can be effectively overcome. And RNAi operation difficulty caused by cocoon wrapping in the pupal stage and short life in the adult stage is avoided. Moreover, the method disclosed by the invention is simple to operate and high in interference efficiency, provides a theoretical basis and a reference effect for further researching gene functions of the parasitic wasps, and has important significance on biological control.

The invention relates to the technical field of agricultural biology, in particular to application of a neuropeptide gene CpILP6 in relieving diapause of codling moths. After siRNA is microinjected to silence CpILP6 in a targeted manner, the diapause state of CpILP6 is changed and tends to a normal development phenotype; the research result of the invention provides a theoretical basis for defining the regulation function of the neuropeptide gene CpILP6 in the diapause induction and maintenance process of codling moth.

The invention discloses a method for improving developmental potential of cloned embryos through high expression of Smarca5 and application of the method. According to the method, after the SCNT embryo is reconstructed, the proportion of 4 cell periods, the blastocyst formation rate and the proportion of inner cell clusters are remarkably increased by microinjection of Smarca5mRNA (500ng / mL). Immunofluorescence and qPCR results show that the expression of EGA related genes such as Polr2a and Dppa2 is up-regulated through high expression of the Smarca5, the synthesis level of new RNA is enhanced, and the abundance of H3K9me3 is remarkably reduced by adjusting the balance of Kdm4b / Suv39h2. According to the method, the embryo epigenetic reprogramming level is improved, the SCNT development quality is remarkably improved, and the method can be widely applied to the fields of animal cloning and embryo engineering.

The invention belongs to the technical field of GPCR drug research and development, and discloses a ratio type cAMP fluorescent probe RaCamp and a construction method of a transgenic mouse, the probe RaCamp is formed by series fusion of G-Flamp2 fluorescent protein and mCherry fluorescent protein through a flexible linker, and the preparation method of the probe RaCamp comprises the following steps: S1, obtaining an mCherry fragment; s2, a G-Flamp2 linear carrier is obtained; s3, homologous recombination; s4, converting and screening; the construction method of the transgenic mouse comprises the following steps: T1, designing a carrier; t2, microinjection of fertilized eggs; t3, embryo transplantation and reproduction; the invention has the beneficial effects that mCherry is added as an internal reference protein on the premise of keeping the sensitivity of the original G-Flamp2 probe, and the selected connecting peptide (GGGGS) 2 enables the expression quantity of the two proteins in cells to be accurately controlled to be 1: 1, so that the problem of background interference in cell observation of in-vivo imaging is well solved. And the sequence is inserted into a Rosa26-LSL box, so that the construction of a Rosa26-LSL-RaCamp mouse is realized.

The utility model relates to the field of injection pumps, in particular to a micro-injection pump placing frame which comprises a base, a moving part is arranged at the bottom of the base, the top of the base is connected with a placing box in a sliding mode through a supporting part, a clamping assembly is arranged in the supporting part, and the clamping assembly is used for adjusting the height of the placing box. Wherein an injection pump is placed in the placing box, the injection pump is connected with an infusion tube, and a storage assembly used for winding the infusion tube is arranged on the side face of the placing box, so that on one hand, the height of the placing box can be conveniently and rapidly adjusted by pressing an unlocking rod to drive a pair of clamping rods to slide; on the other hand, the treatment time of a patient is not affected, the infusion tube can be wound through the storage assembly, the situation that the infusion tube is randomly placed due to lack of fixing measures can be prevented, normal operation of the infusion tube is affected when the injection pump is used is avoided, the structure is simple, operation is easy and convenient, and practicability is high.

Provided herein are methods, computer programs, and systems for automated microinjection, for example, automated Intracytoplasmic Sperm Injection (ICSI), wherein creating, a first dataset of an oocyte and a holding device and a second dataset of an injection pipette; detecting the oocyte and the holding device in the first dataset and the injection pipette; selecting the image of the first dataset and of the second dataset where an equatorial plane of the oocyte / holding device and of the injection pipette has an improved focusing parameter; selecting images of the first and second datasets and labeling the pixels associated with the oocyte and to the injection pipette; creating an injection trajectory for the injection pipette to perform the ICSI using detected morphological structures; detecting when the oocyte is rupturing and when the spermatozoa has been released from the injection pipette into the cytoplasm of oocyte.

The invention discloses a construction method of a COL4A5-K229X point mutation X-linked Alport syndrome mouse model. The construction method comprises the following steps: aiming at c.685Agt of a No.12 exon of a mouse COL4A5 gene; carrying out T point mutation, and designing and preparing Cas9 mRNA, gRNA and a donor vector; the components are mixed and then microinjected into fertilized eggs of a C57BL / 6J mouse to obtain an F0-generation mouse; identifying the genotype through PCR (Polymerase Chain Reaction) amplification and Sanger sequencing, and screening positive mice; mating the positive F0-generation mice with the wild-type mice, and breeding F1-generation and subsequent generations; the phenotype of the model is further verified through qPCR, biochemical analysis, light microscopic examination, transmission electron microscope and immunofluorescence. The model constructed by the invention shows hematuria, proteinuria, azemia, podocyte loss and irregular thickening and layering of glomerular basement membrane, is consistent with phenotypes of human XLAS patients, and provides an animal model tool for analyzing pathogenesis and developing treatment strategies.

The invention belongs to the technical field of transgenic and biomedical research tools, and particularly relates to a method for constructing a transgenic fish system based on a Tol2 transposon system, which comprises the following steps: constructing a myo6b-lhfpl5b-DsRed plasmid by using myo6b as a promoter, lhfpl5b as a target gene and DsRed as a red fluorescent protein tag through a microinjection method, and constructing a Tol2 transposon system-based transgenic fish system. Co-injecting the plasmid containing the Tol2 transposase mRNA and the Tol2 transposase mRNA into a fertilized egg of an AB zebra fish strain, and screening generation by generation to obtain a transgenic fish strain with stable inheritance MET channel gene lhfpl5b marked hair cells. The spatio-temporal dynamic visualization of the living whole fish to the lhfpl5b is realized, the deafness-causing molecular pathway is clarified, and an in-vivo platform is provided for drug screening and intervention strategies.

The application belongs to the technical field of biology and particularly relates to a functional recombinant Cas9 protein targeted to an ovocyte of Procambarus clarkii and application thereof. The protein is NLs-VgSP-Cas9-NLs. The application directly delivers the recombinant Cas9 protein, thereby reducing the risk of integration of exogenous genes. By specifically targeting the ovocyte, off-target effects on somatic cells can be avoided, the efficiency of editing of germ cells is improved, and early development or genetic manipulation can be conveniently studied. The ovocyte of Procambarus clarkii is large, the recombinant Cas9 protein is efficiently delivered by receptor-mediated endocytosis on the surface of the ovocyte, and the problem that microinjection of zygotes cannot be implemented is solved.

The invention provides a method for constructing an organ regeneration genetic compensation effect, and relates to the technical field of biology. Comprising the following steps: S1, designing sgRNA; s2, carrying out in-vitro transcription and purification to obtain a high-purity sgRNA solution; s3, preparing fertilized eggs, preparing sgRNA mixed injection, and performing embryo culture after micro-injection operation to obtain F0-generation animal larvae; s4, performing gene knockout efficiency detection on the F0-generation animal larvae, screening out the F0-generation animal larvae with high knockout efficiency, and breeding and mating the F0-generation animal larvae to obtain F1-generation animals; and S5, verifying and screening the F1-generation animals to obtain homozygous knockout individuals, carrying out amputation treatment on the homozygous knockout individuals, and verifying the genetic compensation effect of organ regeneration. The YAP knockout animal is constructed through a gene editing technology, the effect and mechanism of the YAP knockout animal in limb regeneration are researched, whether YAP deletion affects the regeneration capacity through a genetic compensation effect or not can be revealed, and then a theoretical basis and an experimental basis are provided for future regenerative medicine application.

The invention belongs to the technical field of animal reproductive biology and cell engineering, and particularly relates to a fluorescence labeling expression system for visualizing an oocyte nucleus maturation process and application of the fluorescence labeling expression system. The fluorescence labeling expression system comprises an eukaryotic expression vector pVenus-LMNB1; the eukaryotic expression vector pVenus-LMNB1 comprises a nucleotide sequence for coding the fusion protein Venus-LMNB1. According to the invention, a nuclear fiber layer protein stably expressed on a nuclear membrane is selected as a research object, a eukaryotic expression vector pVenus-LMNB1 is constructed by using a molecular cloning technology, an LMNB1 gene containing a green fluorescent label Venus is overexpressed in a porcine oocyte by using a microinjection technology, fluorescent protein labeling of a structural protein on the nuclear membrane is utilized, and the expression vector pVenus-LMNB1 is constructed. The expression positioning condition of the LMNB1 in the maturation process of the porcine oocytes is observed, and a powerful tool is provided for development of in-vitro culture related researches of the porcine oocytes.

The invention discloses a kelp gametophyte gene editing method and application, and belongs to the technical field of gene editing, the kelp gametophyte gene editing method comprises the following steps: preparing a kelp gametophyte receptor; assembling an RNP complex and preparing a microcarrier; introducing the RNP compound into a kelp gametophyte by using a gene gun; and recovering culture and screening. According to the technical scheme, the dependency of a microinjection technology on precise instruments and professional operation skills is eliminated, efficient gene delivery can be achieved through a standardized gene gun operation process, the technical threshold is greatly reduced, and the kelp gametophyte gene editing method has remarkable technical advantages. According to the method, the high-throughput characteristic of a gene gun technology is fully exerted, multiple mutants can be obtained through one-time bombardment, the experiment efficiency is greatly improved, and powerful support is provided for kelp large-scale gene function research and molecular breeding.

The present application relates to the field of biotechnology, and more particularly to a method for knocking out runx2b gene of heterologous octoploid Changfeng crucian and application thereof. The present application designs gRNA-1 and gRNA-2 aiming at all the alleles of runx2b from two sets of parent sources of heterozygous gynogenetic crucian and Xingguo red common carp in Changfeng crucian; the mixture of two gRNAs synthesized by in vitro transcription and Cas9 protein is introduced into the animal pole of single-cell stage of Changfeng crucian fertilized eggs by microinjection, which can efficiently knock out all the 8 alleles of runx2b-A and runx2b-B. Any gRNA can guide the CRISPR / Cas9 system to simultaneously edit the 8 alleles; gRNA-1 achieves 97.5% of synchronous knockout rate, and gRNA-2 achieves 95% of synchronous knockout efficiency; when the double gRNA collaborative cutting strategy is used, the complete editing efficiency of the 8 functional copies reaches 100%.

The invention discloses application of transgenic zebrafish in preparation of an animal model capable of visually monitoring mast cells. The transgenic zebrafish is prepared by the following method: respectively carrying out XhoI and BamHI double enzyme digestion on a cpa5 gene promoter sequence (SEQ ID NO: 1) and plasmids with fluorescent protein genes and Tol2 transposon recognition sites, and then connecting to obtain recombinant plasmids; and then introducing the recombinant plasmid and Tol2 transposase mRNA into a zebra fish embryo in a microinjection mode in a 1-cell period of zebra fish embryo development, and culturing at constant temperature to obtain the transgenic zebra fish. The cpa5 promoter is constructed to drive an expression framework of the fluorescent protein, so that the mast cells naturally emit fluorescence in the zebra fish body, and an effective animal model is provided for mechanism research and drug screening of mast cell related diseases.

The invention belongs to the technical field of bioengineering and analytical chemistry, and provides a probe eZinCh-2 modified plasmid based on FRET (Fluorescence Resonance Energy Transfer) and application of the probe eZinCh-2 modified plasmid in zinc ion detection. According to the invention, the plasmid is constructed through molecular cloning and homologous recombination technologies, and is microinjected into a zebrafish embryo, so that a transgenic strain capable of stably inheriting and expressing the probe protein is successfully obtained; as a living body biological tool, the zebrafish of the strain is combined with a confocal microscope spectrum FRET technology, and real-time and quantitative monitoring and visual imaging of dynamic changes of zinc ions at living body, cell and tissue levels are achieved. The method solves the problem that real-time dynamic visual monitoring of the zinc ions cannot be realized in a living body model in the prior art, and is suitable for real-time microscopic quantitative analysis of the content of the zinc ions in model organisms such as zebra fish and the like.

This invention provides a research model and construction method for calcium ion imaging of ASH neurons in nematodes, belonging to the field of biological model construction technology. The construction method includes the following steps: nematode culture; construction of a transgenic probe plasmid: obtaining the ASH neuron-specific promoter sra-6 using PCR technology, and connecting HindIII and BamHI restriction sites to both ends of the promoter; amplifying YC3.60 using PCR technology; then inserting the promoter sequence into the pPD95.75 plasmid, and then inserting YC3.60 after the promoter. Transgenic microinjection of nematodes is used, followed by screening to obtain nematodes with ASH neurons carrying green fluorescent protein. In this application, YC3.60 is transferred into nematodes via transgenic means. Using a promoter specifically expressed in ASH neurons to link the gene of this fluorescent probe protein into the nematode, the fluorescent probe can be specifically expressed in the neuron. The constructed nematode model can be used for neuronal structure and functional imaging studies, as well as neuropharmacological efficacy analysis.

The utility model relates to the technical field of scientific research, and discloses a micro-injection pump for brain stereotactic injection, which comprises a base and a controller, the top of the base is rotatably connected with a rotating seat, the top of the rotating seat is fixedly connected with a supporting wheel, the top of the supporting wheel is fixedly connected with a first supporting frame, and the first supporting frame is fixedly connected with a second supporting frame. The top of the first supporting frame is rotationally connected with a first one-way threaded rod, the top of the first one-way threaded rod is rotationally connected with a second supporting frame, the periphery of the first one-way threaded rod is in threaded connection with a movable frame, the interior of the movable frame is in threaded connection with a second one-way threaded rod, and the left side of the second one-way threaded rod is rotationally connected with a first sliding frame. The right side of the first sliding frame is rotationally connected with a second sliding frame. According to the utility model, the rotating handle, the rotating handle I and the rotating handle II can be sequentially rotated, so that the angle, the height and the horizontal position of the injector can be adjusted, the injector can be more accurately aligned with a target brain region, and the flexibility of equipment is improved.

The invention discloses a non-human animal integrated with a double-derived VH subclass fusion gene as well as a construction method and application of the non-human animal. The method comprises the following steps: firstly, constructing a gene construct containing human IGHV1-69 and IGHV3-23 fragments, a specific spacer region, a functional element and a rat heavy chain 3'enhancer, and microinjecting the gene construct into a C57BL / 6J fertilized egg with an endogenous heavy chain and a kappa light chain knocked out to obtain a transgenic mouse; through identification, after a mouse endogenous antibody is inactivated, a human gene is stably integrated, a human IgM antibody is expressed and H5N1 HA immunization is carried out, the use frequency of IGHV1-69 is remarkably increased, the mouse produces a high-titer specific antibody, and serum has neutralizing activity. The mouse can generate a target bnAbs aiming at the HA stem and the RBS in parallel, so that the antibody screening efficiency is improved, and an in-vivo screening and evaluation platform for simulating human body fluid response is provided for research and development of influenza antibodies and vaccines.

The invention provides a preparation method of a genetically modified mouse capable of mediating Tbx1 inherent disorder protein structural domain deletion. The method comprises the following steps: (a) constructing a targeting vector; (b) preparing a gene editing compound; (c) microinjection; (d) embryo transplantation and screening; (e) breeding and establishing a stable strain. Sequencing verifies that the gene modified mouse can stably express the Tbx1 mutant protein which lacks IDR and carries an N-terminal 3xFlag tag, and a homozygote mutant mouse shows remarkable developmental defects such as ventricular septal defect and craniofacial deformity; and an important genetic tool is provided for researching the action mechanism of the phase separation characteristic of the Tbx1 protein in the heart, craniofacial development and congenital heart disease (such as DiGeorge syndrome).

The invention discloses a construction method and application of an Ace2 gene modified golden hamster model, and belongs to the technical field of genetic engineering animal model construction. According to the invention, sgRNA specifically targeting a golden hamster Ace2 gene is prepared, the golden hamster Ace2 gene is accurately targeted by using a two-cell microinjection technology, and gene modification is realized, so that a gene modified hamster is obtained. According to the invention, the golden hamster is used as a model background animal to construct an SARS-CoV-2 research and application tool mouse, and the constructed Ace2-deficient golden hamster model is applied to the field of research and application of a novel coronavirus disease model.

The invention discloses a multifunctional fixing device for mouse ventricular micro-injection and a using method. The multifunctional fixing device comprises an operation table, a height adjusting assembly, a fixing plate, a cranial height adjusting frame, a mounting base, an incisor hook and an ear rod. Wherein the operation table comprises a bottom plate and a vertical plate which are vertically connected, a sinking groove is formed in the bottom plate, and a lifting table is arranged in the groove; the height adjusting assembly is parallel to the vertical plate and close to the bottom plate side; the fixed plate is provided with two symmetrical extension strips at the moving end of the adjusting assembly and on the deviating side, the other surface of the fixed plate is provided with a length adjusting groove, and a first driving piece is arranged in the groove; the cranial height adjusting frame is connected with the first driving piece and provided with a second driving piece. A fixing hole is formed in the far end of the mounting seat; and the incisor hook is arranged on the mounting seat and is positioned above the fixing hole. The two ear rods are symmetrically arranged at the far end of the extension strip. The scheme has the effects of reducing damage to mice, reducing experimental pollution and improving operation stability.

The invention discloses a low-density sperm braking method without a PVP medium in ICSI fertilization, and relates to the field of assisted reproduction therapy, and the method comprises the following steps: step 1, diluting a sperm suspension until the density is 0.1 * 10 < 6 > / ml, and placing the diluted sperm suspension in an in-vitro operating solution without PVP; step 2, under an inverted microscope, directly pressing and braking the tail of the sperm in the in-vitro operating fluid by using a microinjection needle, so that the tail of the sperm is folded or curled to deform; and step 3, transferring the braked sperms into operation liquid drops containing ovum to complete microinjection, and performing tail pressing operation in an isotonic operation liquid without PVP (Polyvinyl Pyrrolidone), so that the tail parts of the sperms present visible curled deformation or tail folding. The sperm motility destruction rate is 100% (residual motility is completely prevented from interfering fertilization), and the tail cell membrane rupture rate is 100% (it is ensured that activation factors in sperms are fully released).

This invention relates to the field of gene editing technology, and more particularly to a method for constructing a zebrafish epilepsy model with htr6 gene deletion. Using CRISPANT technology, this invention designs suitable target sites on the htr6 gene in zebrafish, and co-injects specific sgRNA synthesized in vitro with Cas9 protein into zebrafish fertilized eggs via microinjection, successfully constructing a zebrafish epilepsy model. This invention can more efficiently and accurately silence specific genes in the genome of an organism, and is simple and low-cost to produce. It can also simultaneously cleave multiple sites on the target gene, silencing any number of individual genes. The zebrafish epilepsy model provided by this invention lays a solid foundation for further research on the relationship between htr6 gene mutations and the pathogenesis of epilepsy, as well as for screening antiepileptic drugs.

The application discloses a method for constructing a fluorescently labeled piwil1 gene knockout sterile zebrafish line, which utilizes a CRISPR / Cas9 system to construct a piwil1 gene knock-in vector carrying P2A-EGFP or P2A-mCherry, respectively, to obtain two hybrid strains through microinjection, and then to screen a homozygote expressing EGFP and mCherry simultaneously by mating the two strains. The homozygote shows male sterility and can be used for reproductive development research and ecological prevention and control of transgenic fish. The application realizes visual labeling of the function of the piwil1 gene and accurate screening of the sterile phenotype, and is simple and efficient to operate.

The invention relates to the technical field of gene engineering, in particular to a Cilp gene enhancer and application thereof. Through integration and verification of an ATAC-seq map, a single cell transcriptome data set and multi-omics data, the Cilp gene enhancer is obtained through screening, and a mouse inner ear microinjection system is utilized to further prove that the Cilp gene enhancer can significantly enhance the transcriptional activity of an EGFP protein gene in cochlea type 2 neuronal cells. Compared with other sequences with similar sizes, the Cilp gene enhancer provided by the invention can drive specific expression of a reporter gene or a Cilp gene in cochlear neurons, especially type 2 neurons, and the gene expression level is remarkably improved. The Cilp gene enhancer provided by the invention is suitable for biological materials such as recombinant DNA (deoxyribonucleic acid), vectors or adenoviruses, can be used for promoting transcription of EGFP (enhanced green fluorescent protein) genes or other genes in cochlea neurons, and provides a new targeting tool for gene therapy of hereditary hearing loss.

The invention relates to the technical field of microinjection, and discloses a self-adaptive dynamic adjustment automatic microinjection method, which comprises the step of transferring cells with mark point arrays formed on the surfaces in a culture dish containing marking substances. The temperature, humidity and vibration parameters of the environment where the cells are located are collected in real time through multiple sensors. The cells are irradiated with light sources of different wavelengths, and images are captured. Acquiring position information of cell surface mark points, and establishing and optimizing a cell three-dimensional dynamic model. And determining an injection target, planning a path and generating a control instruction. The flexible injection needle is controlled to move and inject substances, the cell state and environmental parameters are monitored in real time during injection, the upper computer adjusts related parameters according to a self-adaptive control algorithm, and the needle is withdrawn to the initial position after injection. The micro-injection precision and stability can be effectively improved, cell damage is reduced, the experiment success rate is increased, and powerful support is provided for cell research and biotechnology application.

The invention discloses a rat and mouse laparoscope operating system which comprises a supporting body, an imaging assembly and an operating assembly, the imaging assembly is connected to the supporting body, electrically connected with external display equipment and used for collecting and transmitting image information, the operating assembly comprises functional parts and a driving part, and the functional parts comprise tweezers, scissors and an injection needle. The tweezers, the scissors and the injection needle are detachably connected with the supporting body, and the driving part is connected to the supporting body, can be in transmission connection with the tweezers and the scissors and is used for driving the tweezers and the scissors to move relative to the supporting body. The visual laparoscope micro-injection system can effectively solve the problem that in the prior art, a visual laparoscope micro-injection system which is suitable for rats and mice, can be independently operated by a single person and has the functions of abdominal cavity micro-injection and surgical operation is lacked.