38 results about "Sperm" patented technology

The invention relates to the field of distant hybridization breeding, and discloses an artificial breeding method for hybrid species of humpback perches and epinephelus fuscoguttatus, which comprises the following steps: selecting healthy humpback perches as female parent fishes, and screening healthy epinephelus fuscoguttatus as male parent fishes; carrying out intensive cultivation on the parents by means of nutrient enrichment and temperature regulation and control, and promoting synchronous development and maturation of gonads of the parents; after the parents reach the breeding state, eggs of the female parent fishes and seminal fluid of the male parent fishes are collected respectively, fertilized eggs are obtained through artificial insemination, incubation management such as temperature control and continuous oxygenation is conducted on the fertilized eggs, and finally hybrid grouper fries are obtained. The crossbreeding application of the humpback perch as the female parent and the epinephelus fuscoguttatus is realized, and the problems that in the traditional hybridization process, parent gonad development is asynchronous, and filial generations cannot develop are effectively solved; the bred hybrid provides technical guarantee for standardization and precision promotion of grouper crossbreeding, and creates favorable conditions for breeding new hybrid lines with excellent characters of parents.

This invention discloses a method for large-scale breeding of all-female triploid mandarin fish, comprising the following steps: (1) Preparation of all-female diploid fertilized eggs: using male mandarin fish pseudo-males (XX) induced by androgens and female mandarin fish (XX) selected from superior strains as parent fish, collecting sperm and eggs, mixing the two, adding water at a temperature of 28℃ to activate sperm for fertilization, and obtaining all-female diploid fertilized eggs; (2) Heat shock induction of triploids: after the all-female diploid fertilized eggs are fertilized in the above-mentioned water at a temperature of 28℃ for 8 minutes, they are immediately transferred to water at a temperature of 39~41℃ for 1~2 minutes for heat shock induction, and all-female triploid fertilized eggs are obtained; (3) Seedling hatching and cultivation: the fertilized eggs induced by heat shock in step (2) are transferred to the hatching system for hatching, and then conventional seedling cultivation is carried out to obtain all-female triploid mandarin fish seedlings. The mandarin fish fry produced by this method have a high female rate, triploid rate, and survival rate.

This invention discloses a method for identifying the parentage between assisted reproductive embryos and sperm or egg donors, belonging to the field of paternity testing technology. The method includes the following steps: S101: Obtaining a single-cell amplification product sample S and sperm / egg donor samples F / M; S102: Sequencing the dimorphic loci of the two samples to obtain DNA data N(S) and N(F / M); S103: Using the loci in the statistical locus set N(S)∩N(F / M) as valid loci; S104: Calculating the mismatch rate and correlation between the two samples based on the valid loci; S105: Determining the parentage between the two samples based on the mismatch rate and correlation; if the correlation ∈ [0.45, 0.60] and the mismatch rate ∈ [0, 3.125%], then the two samples are determined to be parent-child. This patent can accurately determine the parentage between the embryo and the sperm / egg donor even when the genome may be lost.

The present disclosure provides a microscopic insemination support device having a simple configuration. The present disclosure relates to a microscopic insemination support device that supports microscopic insemination in which sperm held by a sperm injection tool is injected into an egg held by an egg holding device. The microscopic insemination support device comprises an acquisition unit configured to acquire information including a relative positional relationship between the sperm injection tool and the egg, and a control unit configured to, on the basis of the information acquired by the acquisition unit, control movement of a stage on which the egg holding device is disposed so as to perform microscopic insemination by injecting sperm into the egg.

The invention relates to a real-time testis rare sperm auxiliary identification method and device which are used for testis incision sperm extraction under a microscope, and the method comprises the following steps: obtaining first real-time image dynamic data; inputting the first real-time image dynamic data to a rare testis sperm auxiliary identification model to obtain rare testis sperm data; and updating the first real-time image dynamic data based on the rare testis sperm data to obtain second real-time image dynamic data. The method has the advantages that due to the fact that the auxiliary identification model for the rare sperms of the testis is high in response speed, the first real-time image dynamic data can be rapidly identified and updated, and the problems of labeling dislocation, repeated identification and the like cannot occur.

This invention discloses a method for extracting high-purity PDRN from salmon testes and its application in improving skin condition, belonging to the field of bioactive ingredient extraction technology. Frozen salmon testis tissue is cleaned of blood vessels and fatty impurities, then cryogenically pulverized at -20~-25℃. The pulverized tissue is added to a pre-treatment dispersion buffer and homogenized under high pressure to obtain a DNA fragmentation solution. The precipitate is then separated by centrifugation. The precipitate is dispersed in a dedicated enzyme digestion buffer, and enzymatic digestion with protease and RNase A is performed to inactivate the enzyme. DNase I is added to the digestion solution for enzymatic cleavage. After the reaction, a stop solution is added to terminate the reaction, and the supernatant is separated by centrifugation. Ethanol is added to the supernatant for precipitation, and centrifugation yields PDRN from salmon testes. This invention, through specific buffer systems and enzymatic digestion conditions, increases the PDRN content by 40~60 kDa, allowing for better absorption and utilization by the skin, improving facial hydration and elasticity, effectively reducing facial wrinkles, and improving facial skin condition. It can be used to prepare functional cosmetics.

The utility model provides an animal sperm injector convenient to use, and relates to the technical field of animal artificial insemination. The animal semen injection device convenient to use comprises a semen injection supporting rod body, a semen injection elastic outer sleeve, an easily-folded leakage-proof piece and a blocking piece. The sperm injection elastic outer sleeve is arranged on the sperm injection supporting rod body in a sleeving mode, the two ends of the sperm injection elastic outer sleeve are connected with the sperm injection supporting rod body in a sealed mode, and a containing cavity used for containing sperm is formed between the sperm injection supporting rod body and the sperm injection elastic outer sleeve; the front end of the sperm injection supporting rod body is provided with a first mounting hole for mounting the easy-to-break leakage-proof piece, and the first mounting hole is communicated with the accommodating cavity; a second mounting hole for mounting the blocking piece is formed in the tail end of the semen injection supporting rod body, and the second mounting hole is communicated with the containing cavity; and temperature control wax is arranged in the easy-to-fold leakage-proof piece. The technical effect that the fertilization device is convenient to use is achieved.

The invention discloses Lap3a, Lap3b and Lap3c genes of bactrocera cucurbitae and an application of the Lap3a, Lap3b and Lap3c genes. The space-time and tissue expression profiles of the Lap3a, Lap3b and Lap3c genes of the bactrocera cucurbitae show that the three genes are specifically and highly expressed in testis of male bactrocera cucurbitae, especially in transformation regions in testis tissues of adult bactrocera cucurbitae. The Lap3a, Lap3b and Lap3c genes play important functions in the process of participating in maintaining the male reproduction of the bactrocera cucurbitae, when the Lap3a, Lap3b or Lap3c genes of male bactrocera cucurbitae are inhibited, the number of male sperms of the bactrocera cucurbitae is reduced, the reproductive power of the male bactrocera cucurbitae is reduced, the treated male bactrocera cucurbitae is mated with normal female bactrocera cucurbitae, and the egg laying amount and egg hatching rate of the female bactrocera cucurbitae are reduced. The three genes are expected to become SIT potential targets to be applied to pest control, and a new thought is provided for green control of bactrocera cucurbitae.

This invention discloses a highly efficient method for hatching fish adhesive fertilized eggs, specifically including the following steps: Adhesive eggs and sperm are mixed with feathers to form fertilized eggs; 20-40 times their volume of 0.65%-0.9% physiological saline is added, and the mixture is further mixed with feathers to form a fertilized egg mixture; the egg-collecting part is horizontally placed in the hatching tank using a control mechanism, with the egg-collecting part 10-20 cm above the water surface; the fertilized egg mixture is then poured into a funnel, and the funnel is moved back and forth and side to side to ensure the fertilized egg mixture flows evenly onto the egg-collecting part; the egg-collecting part is rotated using a control mechanism to ensure that all positions on the egg-collecting part are covered with fertilized eggs; and finally, the egg-collecting part with the fertilized eggs attached is vertically hung in the hatching tank using a control mechanism. The egg-collecting part of this invention includes a straight rod and multiple sets of filaments. The multiple sets of filaments are distributed vertically along the straight rod to form a columnar structure. The columnar structure has a three-dimensional spatial shape, good air permeability, and can collect eggs in different areas, resulting in a high egg carrying capacity. Furthermore, it prevents the fertilized eggs from piling up and squeezing each other, effectively avoiding cross-infection.

The invention discloses chitosan-pDNA nanoparticles and application thereof in induction of pseudo male salmon of Atlantic salmon, and belongs to the technical field of induction of Atlantic salmon strains. The method comprises the following steps: cloning Amh genes of Atlantic salmon to a pIRES2-EGFP vector to obtain plasmids containing the Amh genes; the preparation method comprises the following steps: dissolving chitosan in a 25mM sodium acetate solution according to the concentration of 0.1% (w / v), and adjusting the pH value to 5.0-5.5 by using acetic acid; diluting the concentration of the obtained plasmid to 200ng / mu l to prepare a plasmid solution containing 0.5% (w / v) sodium sulfate; and respectively preheating the two obtained solutions at 55 DEG C, carrying out isovolumetric mixing, carrying out rapid and violent vortex for 55-60 seconds, and standing at room temperature for more than 30 minutes to obtain the chitosan-pDNA nanoparticles. The chitosan-pDNA nano-particles are used for preparing XX type Atlantic salmon pseudo male fish, mature X type sperms are obtained, the X type sperms are fertilized with normal Atlantic salmon X type ova, and Atlantic salmon all-female offspring seeds are obtained.

To provide an intracytoplasmic sperm injection support device with a simple device configuration. [Solution] Sperm held in sperm injection tool 3 is held by egg holding device An intracytoplasmic sperm injection (ICSI) support device 2 that assists in intracytoplasmic sperm injection (ICSI) in which sperm is injected into an egg, comprising a sperm injection tool 3 and an egg An acquisition unit that acquires information including the relative positional relationship with the child, and the information acquired by the acquisition unit Based on this, an egg retention device is positioned to perform intracytoplasmic sperm injection (ICSI) by injecting sperm into the egg. An intracytoplasmic sperm injection support device 2 comprises a control unit that controls the movement of stage 11.

1. The name of the design product: powder receiving ring. 2. The use of the design product: for coffee powder receiving. 3. The design points of the design product: in shape. 4. The picture or photo that best shows the design points: design 1 perspective view. 5. Design 1 is designated as the basic design.

The invention discloses a method for inducing gynogenesis of Sinilabeo notabilis by white crucian carp sperms and application thereof, and the method for inducing gynogenesis of Sinilabeo notabilis by white crucian carp sperms comprises the following steps: (1) collecting and diluting white crucian carp sperms, and then carrying out inactivation treatment by ultraviolet lamp irradiation; (2) mixing the inactivated seminal fluid of the carassius auratus and the inactivated mature eggs of the sinilabeo nolatus, performing dry insemination, then pouring the mixture into cold water for treatment, putting fertilized eggs into a normal-temperature water incubation tank for incubation after cold treatment is finished, and keeping running water to enable the fertilized eggs to float along with water flow and not to sink to the bottom; and (3) after fry are hatched from the fertilized eggs, culturing to obtain gynogenetic sinilabeo nolabeo. According to the method, the carassius auratus sperms are adopted to induce the gynogenesis of the semilabeo nolabeo to successfully breed and obtain the gynogenesis semilabeo nolabeo offspring, which is the first time in the artificial induction gynogenesis and germplasm improvement of the semilabeo nolabeo. In the breeding process, it is found that the 6-month-old semilabeo notabilis has the advantages that the growth speed is high, and the low-temperature resistance is remarkably higher than that of the parent semilabeo notabilis.

The application discloses a sperm extractor, which comprises a main body, a first stimulating part and a driving assembly. The main body is formed with a sperm extraction space and a mounting space which is arranged at intervals with the sperm extraction space; the first stimulating part is arranged in the sperm extraction space, and a stimulating cavity is formed in the first stimulating part; the driving assembly is arranged in the mounting space; the driving assembly comprises a driving motor, a first transmission part which is fixedly matched with the first stimulating part, a second transmission part which is slidingly matched with the first transmission part, a first transmission unit which comprises a worm wheel and a worm, a second transmission unit which is connected between the worm wheel and the first transmission part, and a third transmission unit which is connected between the worm wheel and the second transmission part. The worm is connected with the driving motor, the second transmission unit converts the rotating motion of the worm wheel into the linear motion of the first transmission part along a first axis, and the third transmission unit comprises two gears which are used for driving the second transmission part to rotate around the first axis. The sperm extractor has the advantages of compact structure, composite stimulating effect and improved sperm extraction efficiency.

The invention relates to a method for increasing the lifespan of animal sperm comprising contacting said sperm with an inhibitor of Slo3 potassium channel.The invention also relates to a use of an inhibitor of Slo3 potassium channel, for increasing the lifespan of animal sperm or motility of capacitated animal sperm, comprising contacting an inhibitor of Slo3 potassium channel with said sperm. Moreover, the invention relates to an artificial insemination instrument for use in artificial insemination of an animal, comprising animal sperm in contact with an inhibitor of Slo3 potassium channel.The invention also relates to a method for artificially inseminating an animal using said artificial insemination instrument.Eventually, the invention relates to a method for increasing the fertility of an animal, comprising contacting sperm of said animal with an inhibitor of Slo3 potassium channel; then artificially inseminating said animal with said sperm.

This invention discloses a compound moxa stick for warming yang and unblocking obstruction, its preparation method, and its application in improving reproductive system function. It belongs to the field of external preparations of traditional Chinese medicine. Addressing the problems of limited improvement effects of pure moxa sticks, lack of systematic design in compound moxa stick formulations, unclear synergistic effects between components, large fluctuations in conditioning effects, and difficulty in balancing conditioning effects and safety, this invention uses specific weight proportions of Artemisia argyi, Cyperus rotundus, Alpinia galanga, Cinnamomum cassia, Zingiber officinale, Illicium verum, Cinnamomum cassia twig, Spatholobus suberectus, Caesalpinia sappan, Smilax glabra, Euonymus fortunei, Citrus aurantium, Amomum villosum, Citrus reticulata peel, and Atractylodes lancea to prepare a compound moxa stick. The components synergistically enhance each other, and this product is used to prepare a moxibustion intervention product for improving the reproductive function of mammals. It can stably improve female ovarian reserve function and male asthenospermia, simultaneously regulate reproductive-related hormone levels, repair pathological damage to reproductive organs, and improve the quality of reproductive cells.

The invention provides a method for increasing the hatching rate of fertilized eggs of Epinephelus yunnanensis, and belongs to the technical field of fish culture. The method comprises the following steps: S1, parent cultivation and male transformation: selecting five-year-old Epinephelus lanceolatus without seminal fluid as a male parent to perform indoor circulating water cultivation, and inducing male transformation in a cooperative mode of feeding a feed containing methyltestosterone, injecting follicle-stimulating hormone and periodically increasing and decreasing water temperature in the cultivation process; s2, male fish screening: regularly checking the male conversion rate in the parents in the step S1, screening the male fishes with qualified maturity, and screening the male fishes with qualified sperm maturity from the male fishes with qualified maturity; s3, insemination and incubation: collecting the seminal fluid of the male fishes screened in the step S2 for artificial insemination of the eggs of the epinephelus moara, and incubating the fertilized eggs. According to the method, the problems of small male fish quantity, insufficient seminal fluid and low fertilized egg hatching rate in artificial breeding of groupers are solved.

A method for human sperm vitrification is disclosed, which includes providing liquefied human sperm; having a vitrification medium, where the vitrification medium has (a) a sperm buffer, and (b) a cryoprotectant mixture including a permeable cryoprotectant and a non-permeable cryoprotectant; mixing the liquefied sperm with the vitrification medium; providing a sperm straw and loading the straw with 0.25 to 0.5 mL of the obtained mixture; sealing the straw with heat; and placing the straw vertically in a container with liquid nitrogen to vitrify the mixture. A portable kit is also disclosed, which makes it easy to implement the method. The kit includes a vitrification medium, some straws, a container, usage instructions, among others.

The utility model relates to the technical field of medical instruments, in particular to a sperm collector. The sperm taking device comprises a shell, a telescopic mechanism, a stroke adjusting mechanism and a massage assembly, the telescopic mechanism, the stroke adjusting mechanism and the massage assembly are arranged in the shell, the massage assembly comprises an inner cylinder and a flexible body arranged in the inner cylinder, an opening is formed in one end of the shell, the flexible body is provided with a containing cavity corresponding to the opening, and the flexible body is slidably connected with the shell; the telescopic mechanism comprises a fixed disc connected with the shell, a rotating disc rotationally connected with the fixed disc, a driving assembly connected with the fixed disc, an adjusting piece movably installed on the rotating disc and a connecting rod movably connected with the adjusting piece, the driving assembly is in transmission connection with the rotating disc, and the stroke adjusting mechanism is installed on the rotating disc; the stroke adjusting mechanism is in driving connection with the adjusting piece and can drive the adjusting piece to move on the rotating disc so as to adjust the eccentric distance of the adjusting piece relative to the center of the rotating disc. By the adoption of the mode, the stroke of reciprocating motion can be adjusted, and the experience feeling during use is improved.

The ultralow-temperature cryopreservation method comprises the following steps: taking dwarf at a breeding period, performing drying in shade and variable-temperature stimulation to enable the dwarf to discharge sperms naturally, filtering, centrifuging, adding a cryoprotectant into high-density sperms, mixing, and placing on ice to obtain pretreated sperms; the cryoprotectant is composed of seawater and dimethyl sulfoxide in a volume ratio of 100: (12-20); and placing the pretreated sperms on a liquid nitrogen surface for pre-cooling, and immersing the sperms in liquid nitrogen for storage. According to the cryopreservation method of the dwarf sperms, the dwarf sperms are unfrozen and recovered under the condition of 60 DEG C, after the frozen sperms are fertilized, the rate of D-shaped larvae reaches 80% or above, the cryopreservation method can be suitable for researching the influence of ultralow-temperature cryopreservation on offspring developed by the frozen sperms, and the offspring obtained by the frozen sperms has the good survival rate, growth rate and the like.

The present application belongs to the technical field of aquaculture, and particularly relates to a goldfish insemination medium and a method for artificial insemination and hatching of goldfish. The specific implementation steps are as follows: selecting female and male parent fish which are in the process of tail chasing reproduction, squeezing the abdomen of the fish to discharge the eggs and sperm into the insemination medium, and stirring and mixing the artificial insemination; transferring the eggs to a mucus removal medium to remove the mucus of the eggs; placing the mucus-removed eggs into a hatching bag after simple rinsing; and transferring the hatched fry into a rearing pond for feeding. The suitable osmotic pressure and egg white protein environment provided by the insemination medium can significantly prolong the duration of the activity of goldfish eggs and sperm, and improve the effect of increasing the fertilization rate of the eggs; the mucus removal medium is used to remove the mucus of the eggs, so as to avoid the mutual adhesion of the eggs into groups or clusters, improve the hatching rate of the goldfish, and enable the hereditary ornamental traits of the goldfish to fully develop, while preventing pathogenic microorganisms from being transmitted from the parent generation to the offspring generation. The selection of the hatching bag provides a good hatching environment for the eggs.

The invention belongs to the technical field of biology, and particularly relates to a cryopreservation method for germplasm resources of rare aquatic mammals, in particular to a cryopreservation and resuscitation method for seminal fluid of a Yangtze river cowfish. By systematically optimizing the components of the diluent, the proportion of the cryoprotectant and the cooling recovery procedure, the method aims to break through the bottleneck in the prior art, and provides reliable technical support for long-term and efficient preservation and utilization of germplasm resources of the Yangtze River cowfish.

This invention concerns the Separation of Sperm with Superior Sperm Without Centrifugation (SUNs). Technological inventions related to sperm preparation without centrifugation have also been disclosed, but existing inventions still have weaknesses and limitations, including a more complicated manufacturing process, availability of goods, and relatively high prices. This proposed invention is intended to overcome the problems stated above by making a simpler sperm preparation tool without centrifugation. In this method, 1 ml of sperm is placed in the outer tube, 1.2 ml of preparation medium is put into the inner tube until it is full and then some of it moves to the outer chamber. This tool is then closed and transferred to an incubator at 37oC for 60 minutes. During the incubation period, the sperm will move against gravity towards the surface of the liquid and the inner chamber. The sperm is then harvested from the inner tube of the tool.

The application belongs to the technical field of immune detection, and specifically discloses a SMCP polyclonal antibody, a preparation method and application thereof. The preparation method comprises the following steps: using a polypeptide containing an amino acid sequence as shown in SEQ ID NO:1 as an immunogen to immunize animals, collecting whole blood of the immunized animals, and separating to obtain a SMCP polyclonal antibody. The application has the beneficial effect that: the specific SMCP protein antigen epitope is used as the immunogen to immunize animals, which is beneficial to large-scale preparation of the antibody specifically recognizing the SMCP protein. The SMCP antibody prepared by the method has good specificity, can be used for WB, IF and Co-IP experiments, and provides an important research tool for studying the pathogenic mechanism of weak sperm caused by SMCP deletion.

The application relates to a method for preserving animal sperm. The method uses an animal sperm liquid preservation diluent to preserve animal sperm; the animal sperm liquid preservation diluent is prepared by the following method: (1) mixing glucose, fructose, tris-hydroxymethyl aminomethane, citric acid and water to obtain a mixture 1; the mass-volume ratio of the glucose, fructose, tris-hydroxymethyl aminomethane, citric acid and water is (10-20) g:(10-20) g:(20-30) g:(10-20) g:(50-100) ml; (2) mixing edible mixed vegetable oil and the mixture 1 of step (1), stirring, and the volume ratio of the edible mixed vegetable oil and water is 50:(50-100) to obtain a mixture 2; standing, discarding the upper oil, refrigerating, and diluting with sterilized distilled water. The application has the advantages of wide application range, high pregnancy rate, long animal sperm liquid preservation time, good preservation activity and the like.

The invention discloses a method for producing all-male largemouth micropterus salmoides based on soaking induction of sex reversal of largemouth micropterus salmoides, and particularly relates to the field of artificial propagation of all-male largemouth micropterus salmoides, the method comprises the following steps: S01, taking fairy shrimps as initial feed when largemouth micropterus salmoides fries obtained by incubation swim flatly; s02, transferring the largemouth micropterus salmoides fry into a water body in which a sexual reversal induction reagent is dissolved, keeping feeding three times every day during the period, and soaking and breeding the fry for 60 days; and S03, after soaking and culturing for 60 days, transferring the largemouth bass fry into a conventional culture water body for culturing, and after sexual maturity of the largemouth bass fry, obtaining a male population capable of generating seminal fluid, namely the all-male largemouth bass. According to the method, largemouth bass fries are soaked in a sex reversal induction reagent with a specific concentration, sex reversal fishes with a high survival rate and a low malformation rate can be directly obtained, and then large-scale cultivation of all-male largemouth bass is achieved.

The invention provides an artificial propagation method for sculpin versicolor, and belongs to the technical field of fish culture. The artificial spawning induction method comprises the following steps that spawning induction hormone is injected into female parents for breeding parent fishes for three times for artificial spawning induction, the ratio of the female parent fishes to the male parent fishes is adjusted to be (2-3): 1, and fertilized eggs are obtained. The fertilized eggs are placed in a hatching basket to be hatched, a sunshade net is built above the fertilized eggs to shield light, the water temperature during hatching is 10-15 DEG C, dissolved oxygen is 5-10 mg / L, and larva fishes are obtained. According to the method, spawning induction hormone is injected in a three-needle injection mode, the injection dosage and the interval time of each needle are strictly controlled, eggs and seminal fluid which are well developed can be obtained, meanwhile, the hatching conditions of the fertilized eggs are controlled, the spawning induction rate of sculpin versicolor reaches 84.03%, the fertilization rate reaches 93.5% or above, and the hatching rate reaches 87% or above; the small-scale artificial propagation of the sculpin is realized, and the method has important significance for protecting the endangered sculpin which is an endangered resource.

The invention relates to the field of health assessment, in particular to a personalized health assessment system based on an essence, qi and spirit three-dimensional life model, which comprises an essence, qi and spirit three-dimensional model construction module, a multi-source health data acquisition module, a data conversion and standardization module, a health assessment and prediction module and a personalized health management scheme generation module. According to the personalized health assessment system based on the essence, qi and spirit three-dimensional life model, through the multi-source health data acquisition module and the data conversion and standardization module, data from various sources such as a wearable device, a physical examination report and an environment sensor can be integrated in real time; and the data are uniformly mapped to a sperm, qi and spirit three-dimensional model by utilizing a Min-Max standardization and weighted fusion algorithm, so that a comprehensive and consistent health assessment basis is provided.

The invention discloses a preparation method of superfemale acipenser schrenckii, and belongs to the technical field of fish germplasm improvement and innovation, and the preparation method specifically comprises the following steps: obtaining mature acipenser schrenckii eggs and acipenser schrenckii semen through artificial spawning induction; diluting the semen; inactivating the sperms; combining the seminal fluid with the ovum; hydrostatic pressure treatment; hatching fertilized eggs; domesticating the fish fries; sex identification and screening; and detecting the gender genotype of the gynogenetic progeny individual. Through the steps, the superfemale sturgeon individuals can be obtained. By utilizing a gynogenesis technology to improve the variety of the small body sturgeons, a super-female parent core breeding population can be rapidly prepared, the breeding period is obviously shortened, and the breeding cost is saved. Successful sturgeon gynogenesis induction has important significance on sturgeon all-female culture mode construction and sustainable development of caviar industry, and the problem that a technical method for effectively constructing sturgeon all-female culture groups is lacked in an existing breeding technology is solved.

A sperm sampler according to the present design has a core member made of a gel resin, a cap that is removably attached to one end part of the core member, a cover member that covers the other end part of the core member and the outer surface, and a peripheral side plate made of a thin hard resin plate that is provided along the left-right direction from the other longitudinal end; the cover member has a flexible resin sheet and diamond-shaped or triangular tile members provided on the surface of the resin sheet; the tile members are spaced apart from one another.