9 results about "Sperm" patented technology

This invention discloses a method for large-scale breeding of all-female triploid mandarin fish, comprising the following steps: (1) Preparation of all-female diploid fertilized eggs: using male mandarin fish pseudo-males (XX) induced by androgens and female mandarin fish (XX) selected from superior strains as parent fish, collecting sperm and eggs, mixing the two, adding water at a temperature of 28℃ to activate sperm for fertilization, and obtaining all-female diploid fertilized eggs; (2) Heat shock induction of triploids: after the all-female diploid fertilized eggs are fertilized in the above-mentioned water at a temperature of 28℃ for 8 minutes, they are immediately transferred to water at a temperature of 39~41℃ for 1~2 minutes for heat shock induction, and all-female triploid fertilized eggs are obtained; (3) Seedling hatching and cultivation: the fertilized eggs induced by heat shock in step (2) are transferred to the hatching system for hatching, and then conventional seedling cultivation is carried out to obtain all-female triploid mandarin fish seedlings. The mandarin fish fry produced by this method have a high female rate, triploid rate, and survival rate.

This invention discloses a compound moxa stick for warming yang and unblocking obstruction, its preparation method, and its application in improving reproductive system function. It belongs to the field of external preparations of traditional Chinese medicine. Addressing the problems of limited improvement effects of pure moxa sticks, lack of systematic design in compound moxa stick formulations, unclear synergistic effects between components, large fluctuations in conditioning effects, and difficulty in balancing conditioning effects and safety, this invention uses specific weight proportions of Artemisia argyi, Cyperus rotundus, Alpinia galanga, Cinnamomum cassia, Zingiber officinale, Illicium verum, Cinnamomum cassia twig, Spatholobus suberectus, Caesalpinia sappan, Smilax glabra, Euonymus fortunei, Citrus aurantium, Amomum villosum, Citrus reticulata peel, and Atractylodes lancea to prepare a compound moxa stick. The components synergistically enhance each other, and this product is used to prepare a moxibustion intervention product for improving the reproductive function of mammals. It can stably improve female ovarian reserve function and male asthenospermia, simultaneously regulate reproductive-related hormone levels, repair pathological damage to reproductive organs, and improve the quality of reproductive cells.

The application belongs to the technical field of immune detection, and specifically discloses a SMCP polyclonal antibody, a preparation method and application thereof. The preparation method comprises the following steps: using a polypeptide containing an amino acid sequence as shown in SEQ ID NO:1 as an immunogen to immunize animals, collecting whole blood of the immunized animals, and separating to obtain a SMCP polyclonal antibody. The application has the beneficial effect that: the specific SMCP protein antigen epitope is used as the immunogen to immunize animals, which is beneficial to large-scale preparation of the antibody specifically recognizing the SMCP protein. The SMCP antibody prepared by the method has good specificity, can be used for WB, IF and Co-IP experiments, and provides an important research tool for studying the pathogenic mechanism of weak sperm caused by SMCP deletion.

The application relates to a method for preserving animal sperm. The method uses an animal sperm liquid preservation diluent to preserve animal sperm; the animal sperm liquid preservation diluent is prepared by the following method: (1) mixing glucose, fructose, tris-hydroxymethyl aminomethane, citric acid and water to obtain a mixture 1; the mass-volume ratio of the glucose, fructose, tris-hydroxymethyl aminomethane, citric acid and water is (10-20) g:(10-20) g:(20-30) g:(10-20) g:(50-100) ml; (2) mixing edible mixed vegetable oil and the mixture 1 of step (1), stirring, and the volume ratio of the edible mixed vegetable oil and water is 50:(50-100) to obtain a mixture 2; standing, discarding the upper oil, refrigerating, and diluting with sterilized distilled water. The application has the advantages of wide application range, high pregnancy rate, long animal sperm liquid preservation time, good preservation activity and the like.

This invention discloses a method for interspecific hybridization and seedling cultivation of *Pterocarya spp.* and *Pterocarya martensii*, comprising: selecting mature, live male *Pterocarya spp.*, cleaning and drying them, placing them in seawater, continuously aerating and artificially circulating the water to induce natural sperm release; dissecting mature female *Pterocarya martensii* to extract gonads, collecting mature eggs, cleaning them, and allowing them to settle to obtain an egg mixture; adding ammonia to the egg mixture to activate the eggs; adding the activated egg mixture to a sperm-containing water body for fertilization in the dark; after successful fertilization, removing the male *Pterocarya spp.* and transferring the fertilized eggs to a seedling pond; and cultivating the fertilized eggs in seawater to develop into D-shaped larvae. This invention is the first of its kind for distant hybridization and seedling cultivation of *Pterocarya spp.* and *Pterocarya martensii*, breaking through the reproductive isolation between the two species, achieving stable and efficient hybridization and fertilization. The process is simple and highly stable, and can cultivate new hybrid pearl oyster seedlings that combine the excellent traits of both parents. It has significant implications and promotional value for the innovation of marine pearl oyster germplasm and industrial upgrading.

The utility model relates to the technical field of sperm-egg co-culture bin, concretely is sperm-egg co-culture bin with constant temperature maintaining function, the utility model discloses, including co-culture bin, object carrier and fixing device, one side of co-culture bin is fixedly connected with control panel, the inner wall of co-culture bin is equipped with exhaust device, the exhaust device includes several slide rails and object carrier, the upper surface of several object carriers is connected with object carrier by the fixing device, several slide rails are fixedly connected with co-culture bin, the inner wall of slide rail is connected with object carrier slidingly, one end of slide rail is fixedly connected with fixed block, the upper surface of fixed block is fixedly connected with telescopic link, the other end of telescopic link is fixedly connected with connecting plate. Solve the problem that the traditional sperm-egg co-culture bin is not observed because of the traditional sperm-egg co-culture bin when using traditional sperm-egg co-culture bin to co-culture, thereby in co-culture, it is easy to cause the problem of co-culture error.

A rotary telescopic semen collector includes a housing, a massage section, and a drive device. The massage section includes a first soft gel body and a second soft gel body spaced apart within the housing. The first soft gel body is located in front of the second soft gel body, which is rotatably disposed within the housing. The drive device can drive the first soft gel body to move linearly along the penis with the first soft gel body inserted, and can also drive the second soft gel body to rotate axially relative to the housing. The rotary telescopic semen collector provided by this application can provide telescopic massage to the middle of the penis and rotary massage to the head of the penis, thus diversifying the massage functions and using fewer components with a simple structure. Users can adjust the movement speed of the first and second soft gel bodies separately by controlling the drive device to achieve a more comfortable massage effect. This provides users with more massage combinations, meeting the needs of different users and improving the applicability of the rotary telescopic semen collector.