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116 results about "Sperm" patented technology

Sperm is the male reproductive cell. In the types of sexual reproduction known as anisogamy and its subtype oogamy, there is a marked difference in the size of the gametes with the smaller one being termed the "male" or sperm cell. A uniflagellar sperm cell that is motile is referred to as a spermatozoon, whereas a non-motile sperm cell is referred to as a spermatium. Sperm cells cannot divide and have a limited life span, but after fusion with egg cells during fertilization, a new organism begins developing, starting as a totipotent zygote. The human sperm cell is haploid, so that its 23 chromosomes can join the 23 chromosomes of the female egg to form a diploid cell. In mammals, sperm develops in the testicles, is stored in the epididymis, and released from the penis.

Characterizing sperm and correlating their DNA

A sorting track for sorting living sperm includes a housing that defines at least two cubicles, an initial placement location, and a channel fluidly coupled to the at least two cubicles and the initial placement location such that the at least two cubicles each feature a unique set of cubicle characteristics, an extraction portal, and an entrance fluidly coupled to the channel. Cubicle characteristics may include one or more architectural characteristics selected from the group consisting of: a distance from the initial placement location, a cubicle shape, a cubicle volume, a cubicle position within the housing relative to the channel, an entrance size, and an entrance shape. Cubicle characteristics may also include stimulus characteristics selected from the group consisting of: a wavelength of light, a light intensity, a radioactive radiation intensity, an electromagnetic field, a chemical treatment, a pressure, a sound, and a temperature.
Owner:GETMAN ANYA L

High-temperature-resistant saccharomyces cerevisiae strain as well as construction method and application thereof

The invention relates to a high-temperature-resistant saccharomyces cerevisiae strain and a construction method and application thereof in the field of microorganisms. The high-temperature-resistant saccharomyces cerevisiae strain disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.27605, and the preservation name is SEB22. The SEB22 bacterial strain is obtained by knocking out an L-asparaginase II coding gene ASP3 and a high-expression transcription factor CRZ1 from an original bacterial strain SEB4. The glucose consumption rate, the ethanol yield and the yield of the SEB22 strain under high-temperature stress are obviously improved, and the SEB22 strain has good application potential in the aspect of high-temperature SSF fermentation.
Owner:CHINA PETROLEUM & CHEMICAL CORP +2

Methods for evaluating rheotaxis quality in a sperm-containing sample and systems therefor

The present technology relates to a systems for quantifying rheotaxis in a sperm-containing sample. The system includes a microfluidic system having an inlet for charging fluids into a passage. One or more probes defining a confinement region suitable for retaining motile sperm are within the passage. The system further comprises an image processing computing device for obtaining a sequence of images of the confinement region of at least one of the one or more probes having motile sperm retained therein. The sequence of images of the confinement region is processed to determine a signal intensity value for said sequence of images, wherein said signal intensity value is based on a concentration of the motile sperm located in the confinement region at the flow rate. A rheotaxis quality value is determined for said sperm-containing sample based on the signal intensity value. Methods for quantifying rheotaxis in a sperm-containing sample are also disclosed.
Owner:CORNELL UNIVERSITY

Artificial breeding method for hybrid species of humpback perches and epinephelus fuscoguttatus

The invention relates to the field of distant hybridization breeding, and discloses an artificial breeding method for hybrid species of humpback perches and epinephelus fuscoguttatus, which comprises the following steps: selecting healthy humpback perches as female parent fishes, and screening healthy epinephelus fuscoguttatus as male parent fishes; carrying out intensive cultivation on the parents by means of nutrient enrichment and temperature regulation and control, and promoting synchronous development and maturation of gonads of the parents; after the parents reach the breeding state, eggs of the female parent fishes and seminal fluid of the male parent fishes are collected respectively, fertilized eggs are obtained through artificial insemination, incubation management such as temperature control and continuous oxygenation is conducted on the fertilized eggs, and finally hybrid grouper fries are obtained. The crossbreeding application of the humpback perch as the female parent and the epinephelus fuscoguttatus is realized, and the problems that in the traditional hybridization process, parent gonad development is asynchronous, and filial generations cannot develop are effectively solved; the bred hybrid provides technical guarantee for standardization and precision promotion of grouper crossbreeding, and creates favorable conditions for breeding new hybrid lines with excellent characters of parents.
Owner:SANYA INST OF OCEANOGRAPHY OCEAN UNIV OF CHINA

Preparation method and application of high-activity raw material for extracting salmon salmon sperm polydeoxyribonucleotide PDRN (DNA sodium)

PendingCN121221448ACosmetic preparationsSugar derivativesIchthyobodo salmonisAnimal science
The invention provides a preparation method and application of a high-activity salmon salmon sperm PDRN (DNA sodium) raw material. The preparation method comprises the following steps: crushing and freezing a salmon testis at an ultralow temperature, adding a mixed solution of a Tris-HCl buffer solution and sodium chloride, and dispersing at a low temperature; adding a compound enzyme preparation with the total enzyme activity of more than or equal to 350KU / L, and carrying out low-temperature appropriate pH enzymolysis; and adjusting the pH value to a suitable range, and carrying out frequency conversion ultrasonic and high-pressure homogenization alternate synergistic treatment in an ice-water bath to obtain the DNA fragmentation liquid, so that the defects of low PDRN activity retention rate, insufficient purity and high protein residue in the prior art are overcome.
Owner:TIANJIN SAIMENG BIOTECHNOLOGY CO LTD +2

Kidney-tonifying and essence-securing traditional Chinese medicine pill and preparation method thereof

The invention belongs to the field of kidney tonifying and essence securing traditional Chinese medicine, and discloses kidney tonifying and essence securing traditional Chinese medicine pills and a preparation method thereof.The kidney tonifying and essence securing traditional Chinese medicine pills are prepared from, by mass, 15 parts of herba cistanche, 12 parts of radix morindae officinalis, 20 parts of radix rehmanniae praeparata, 15 parts of semen cuscutae, 12 parts of flatstem milkvetch seeds, 10 parts of fructus corni, 10 parts of fructus rosae laevigatae, 10 parts of fructus rubi, 12 parts of mulberries, 15 parts of rhizoma dioscoreae, 10 parts of poria cocos, 6 parts of fructus schizandrae and 6 parts of honey-fried licorice roots. The prescription has the effects of tonifying yin and yang, consolidating diarrhea and harmonizing spleen and kidney; the traditional Chinese medicine has the effects of tonifying kidney and replenishing essence, securing essence, regulating metabolism and delaying senescence of a reproductive system.
Owner:杨润泽

Construction method and application of pig whole sperm membrane protein antigen specific nano antibody library

The invention discloses a pig whole sperm membrane protein antigen specific nano antibody library construction method which comprises the following steps: S1, immunizing alpaca with pig whole sperms, and separating peripheral blood cells after immunization; s2, RNA in peripheral blood cells is extracted, cDNA is obtained through reverse transcription, and a VHH coding sequence of an antibody is amplified; s3, cloning the amplified VHH coding sequence to a bacteriophage plasmid vector to prepare a recombinant bacteriophage vector; s4, transforming the recombinant bacteriophage vector into escherichia coli and carrying out enlarged culture to obtain a bacteriophage display library; and S5, performing panning on the phage display library for a plurality of times by using the pig X / Y sperms, and constructing the pig single sex sperm specific nano antibody library. According to the method, the pig whole sperm is adopted as an immunogen for immunizing alpaca for the first time, the capacity of the constructed antibody library reaches 3.4 * 10 < 8 > pfu, the abundance of the antibody library is higher, the coverage degree of whole sperm antigens is higher, and the variety is more complete, so that the capture success rate of the pig sex control specific antibody is increased, and a foundation is laid for industrial application of the pig sperm specific antibody.
Owner:WENS FOODSTUFF GROUP CO LTD +2

Boar sperm motility miRNA markers and uses thereof

The application discloses six boar sperm motility miRNA markers, which are ssc-miR-486, ssc-miR-10386, ssc-miR-708-5p, ssc-miR-122-5p, ssc-miR-199a-3p and ssc-miR-31, wherein the ssc-miR-486, ssc-miR-10386, ssc-miR-708-5p, ssc-miR-122-5p and ssc-miR-199a-3p are highly expressed in low-motility semen sperm plasma exosomes and lowly expressed in high-motility semen sperm plasma exosomes, and the ssc-miR-31 is lowly expressed in low-motility semen sperm plasma exosomes and highly expressed in high-motility semen sperm plasma exosomes. The application has good prospects for identifying boar sperm motility, and the disclosed miRNA markers have accuracy and reliability.
Owner:FOSHAN UNIVERSITY

Method for mass production of all-female triploid mandarin fish

PendingCN122319994AHeat shockAnimal science
This invention discloses a method for large-scale breeding of all-female triploid mandarin fish, comprising the following steps: (1) Preparation of all-female diploid fertilized eggs: using male mandarin fish pseudo-males (XX) induced by androgens and female mandarin fish (XX) selected from superior strains as parent fish, collecting sperm and eggs, mixing the two, adding water at a temperature of 28℃ to activate sperm for fertilization, and obtaining all-female diploid fertilized eggs; (2) Heat shock induction of triploids: after the all-female diploid fertilized eggs are fertilized in the above-mentioned water at a temperature of 28℃ for 8 minutes, they are immediately transferred to water at a temperature of 39~41℃ for 1~2 minutes for heat shock induction, and all-female triploid fertilized eggs are obtained; (3) Seedling hatching and cultivation: the fertilized eggs induced by heat shock in step (2) are transferred to the hatching system for hatching, and then conventional seedling cultivation is carried out to obtain all-female triploid mandarin fish seedlings. The mandarin fish fry produced by this method have a high female rate, triploid rate, and survival rate.
Owner:SUN YAT SEN UNIV

Method for identifying the parentage between an assisted reproduction embryo and a sperm or egg donor

This invention discloses a method for identifying the parentage between assisted reproductive embryos and sperm or egg donors, belonging to the field of paternity testing technology. The method includes the following steps: S101: Obtaining a single-cell amplification product sample S and sperm / egg donor samples F / M; S102: Sequencing the dimorphic loci of the two samples to obtain DNA data N(S) and N(F / M); S103: Using the loci in the statistical locus set N(S)∩N(F / M) as valid loci; S104: Calculating the mismatch rate and correlation between the two samples based on the valid loci; S105: Determining the parentage between the two samples based on the mismatch rate and correlation; if the correlation ∈ [0.45, 0.60] and the mismatch rate ∈ [0, 3.125%], then the two samples are determined to be parent-child. This patent can accurately determine the parentage between the embryo and the sperm / egg donor even when the genome may be lost.
Owner:WUHAN LANSHA MEDICAL LAB CO LTD +1

Microscopic insemination support device, microscopic insemination support system, microscopic insemination support program, and egg holding device

PCT designated stageWO2026054093A1MicroscopesBiological testingGynecologyObstetrics
The present disclosure provides a microscopic insemination support device having a simple configuration. The present disclosure relates to a microscopic insemination support device that supports microscopic insemination in which sperm held by a sperm injection tool is injected into an egg held by an egg holding device. The microscopic insemination support device comprises an acquisition unit configured to acquire information including a relative positional relationship between the sperm injection tool and the egg, and a control unit configured to, on the basis of the information acquired by the acquisition unit, control movement of a stage on which the egg holding device is disposed so as to perform microscopic insemination by injecting sperm into the egg.
Owner:ARCS INC

Microfluidic device for separating motile cells from non-motile cells

The present disclosure describes a sperm separating device (1). The device comprises a sample zone (5) configured to receive semen, a collection zone (15) fluidly connected to the sample zone by a migration zone (10). A lid (20) is disposed over a part of the migration zone and the collection zone, wherein the migration zone includes grooves to guide sperm within the semen, and wherein the sperm in the semen migrate via the grooves to the collection zone.
Owner:NEOGENIX BIOSCIENCES

Real-time testis rare sperm auxiliary identification method and device

PendingCN121884335AImage enhancementImage analysisRadiologyTesticular sperm extraction
The invention relates to a real-time testis rare sperm auxiliary identification method and device which are used for testis incision sperm extraction under a microscope, and the method comprises the following steps: obtaining first real-time image dynamic data; inputting the first real-time image dynamic data to a rare testis sperm auxiliary identification model to obtain rare testis sperm data; and updating the first real-time image dynamic data based on the rare testis sperm data to obtain second real-time image dynamic data. The method has the advantages that due to the fact that the auxiliary identification model for the rare sperms of the testis is high in response speed, the first real-time image dynamic data can be rapidly identified and updated, and the problems of labeling dislocation, repeated identification and the like cannot occur.
Owner:SHANGHAI FIRST PEOPLES HOSPITAL

Method for extracting high-purity salmon sperm-nodule-derived pdrn and application thereof in improving skin condition

This invention discloses a method for extracting high-purity PDRN from salmon testes and its application in improving skin condition, belonging to the field of bioactive ingredient extraction technology. Frozen salmon testis tissue is cleaned of blood vessels and fatty impurities, then cryogenically pulverized at -20~-25℃. The pulverized tissue is added to a pre-treatment dispersion buffer and homogenized under high pressure to obtain a DNA fragmentation solution. The precipitate is then separated by centrifugation. The precipitate is dispersed in a dedicated enzyme digestion buffer, and enzymatic digestion with protease and RNase A is performed to inactivate the enzyme. DNase I is added to the digestion solution for enzymatic cleavage. After the reaction, a stop solution is added to terminate the reaction, and the supernatant is separated by centrifugation. Ethanol is added to the supernatant for precipitation, and centrifugation yields PDRN from salmon testes. This invention, through specific buffer systems and enzymatic digestion conditions, increases the PDRN content by 40~60 kDa, allowing for better absorption and utilization by the skin, improving facial hydration and elasticity, effectively reducing facial wrinkles, and improving facial skin condition. It can be used to prepare functional cosmetics.
Owner:NATAI (YANGJIANG) BIOTECHNOLOGY CO LTD

Animal sperm injection device convenient to use

The utility model provides an animal sperm injector convenient to use, and relates to the technical field of animal artificial insemination. The animal semen injection device convenient to use comprises a semen injection supporting rod body, a semen injection elastic outer sleeve, an easily-folded leakage-proof piece and a blocking piece. The sperm injection elastic outer sleeve is arranged on the sperm injection supporting rod body in a sleeving mode, the two ends of the sperm injection elastic outer sleeve are connected with the sperm injection supporting rod body in a sealed mode, and a containing cavity used for containing sperm is formed between the sperm injection supporting rod body and the sperm injection elastic outer sleeve; the front end of the sperm injection supporting rod body is provided with a first mounting hole for mounting the easy-to-break leakage-proof piece, and the first mounting hole is communicated with the accommodating cavity; a second mounting hole for mounting the blocking piece is formed in the tail end of the semen injection supporting rod body, and the second mounting hole is communicated with the containing cavity; and temperature control wax is arranged in the easy-to-fold leakage-proof piece. The technical effect that the fertilization device is convenient to use is achieved.
Owner:WUHAN TIANCHU TECH CO LTD

Sperm collecting device

The invention discloses a sperm collecting device. The sperm collecting device comprises a moving cylinder capable of moving in a reciprocating mode. A rotatable inner sleeve is arranged in the moving cylinder, and at least one end of the inner sleeve is open; a telescopic driving assembly is arranged on the side of the moving cylinder and drives the moving cylinder to do reciprocating motion; a rotary driving assembly is further arranged on the side of the moving cylinder and drives the inner sleeve to rotate relative to the moving cylinder. A flexible cylinder is arranged in the inner sleeve, and at least one end of the inner sleeve is open; the sperm collecting device further comprises a sound structure, the sound structure is arranged on the outer side of the moving cylinder and is static relative to the moving cylinder and the inner sleeve, the sound structure comprises a micro loudspeaker and a plurality of sound outlet holes communicated with the outside, the sound outlet holes are arranged around the inner sleeve, and the central axis formed by arranging the sound outlet holes coincides with the axis of the inner sleeve. And sound waves generated by the micro loudspeaker are transmitted out through the sound outlet hole. The sperm extraction device can promote rapid ejaculation of the user and relieve the nervous emotion of the user during sperm extraction.
Owner:SHENZHEN LOVE SENSE TECH CO LTD

A method for efficiently inducing and rapidly identifying homologous tetraploid crucian carp male-nuclear development offspring

The present application belongs to the technical field of fish breeding, and discloses a method for efficiently inducing and rapidly identifying homologous tetraploid crucian male-nuclear development offspring, comprising the following steps: inducing parturition of female hybrid crucian and male homologous tetraploid crucian, obtaining hybrid crucian ovum, placing the ovum in ovary liquid, inactivating the ovum through ultraviolet lamp irradiation, stirring and uniformly mixing the ovum with sperm of the homologous tetraploid crucian to make the ovum "fertilized", without chromosome doubling treatment, and obtaining survival offspring after hatching and feeding, wherein a pair of oral papillae in the survival offspring is the male-nuclear development offspring. The hybrid crucian ovum selected by the present application contains a heterologous genome itself, has better compatibility, is beneficial to improving the hatching rate, can efficiently induce homologous tetraploid crucian male-nuclear development, and simultaneously utilizes the appearance characteristic of the male-nuclear development individual inheriting a pair of oral papillae from the father to rapidly identify the male-nuclear development offspring, so that the goal of rapidly identifying homologous tetraploid crucian male-nuclear development offspring is achieved, and the present application has important significance in fish genetic breeding.
Owner:HUNAN NORMAL UNIVERSITY +1

Lap3a, Lap3b and Lap3c genes of bactrocera cucurbitae and application of Lap3a, Lap3b and Lap3c genes

The invention discloses Lap3a, Lap3b and Lap3c genes of bactrocera cucurbitae and an application of the Lap3a, Lap3b and Lap3c genes. The space-time and tissue expression profiles of the Lap3a, Lap3b and Lap3c genes of the bactrocera cucurbitae show that the three genes are specifically and highly expressed in testis of male bactrocera cucurbitae, especially in transformation regions in testis tissues of adult bactrocera cucurbitae. The Lap3a, Lap3b and Lap3c genes play important functions in the process of participating in maintaining the male reproduction of the bactrocera cucurbitae, when the Lap3a, Lap3b or Lap3c genes of male bactrocera cucurbitae are inhibited, the number of male sperms of the bactrocera cucurbitae is reduced, the reproductive power of the male bactrocera cucurbitae is reduced, the treated male bactrocera cucurbitae is mated with normal female bactrocera cucurbitae, and the egg laying amount and egg hatching rate of the female bactrocera cucurbitae are reduced. The three genes are expected to become SIT potential targets to be applied to pest control, and a new thought is provided for green control of bactrocera cucurbitae.
Owner:FUJIAN AGRI & FORESTRY UNIV

Efficient hatching method of sticky fertilized eggs of fish

This invention discloses a highly efficient method for hatching fish adhesive fertilized eggs, specifically including the following steps: Adhesive eggs and sperm are mixed with feathers to form fertilized eggs; 20-40 times their volume of 0.65%-0.9% physiological saline is added, and the mixture is further mixed with feathers to form a fertilized egg mixture; the egg-collecting part is horizontally placed in the hatching tank using a control mechanism, with the egg-collecting part 10-20 cm above the water surface; the fertilized egg mixture is then poured into a funnel, and the funnel is moved back and forth and side to side to ensure the fertilized egg mixture flows evenly onto the egg-collecting part; the egg-collecting part is rotated using a control mechanism to ensure that all positions on the egg-collecting part are covered with fertilized eggs; and finally, the egg-collecting part with the fertilized eggs attached is vertically hung in the hatching tank using a control mechanism. The egg-collecting part of this invention includes a straight rod and multiple sets of filaments. The multiple sets of filaments are distributed vertically along the straight rod to form a columnar structure. The columnar structure has a three-dimensional spatial shape, good air permeability, and can collect eggs in different areas, resulting in a high egg carrying capacity. Furthermore, it prevents the fertilized eggs from piling up and squeezing each other, effectively avoiding cross-infection.
Owner:FISHERIES RES INST ANHUI ACAD OF AGRI SCI

Method for inducing gynogenesis of acrossocheilus fasciatus by using opsariichthys bidens sperms

The invention provides a method for inducing gynogenesis of acrossocheilus fasciatus by using opsariichthys bidens sperms, and relates to the technical field of fish genetic breeding, and the method comprises the following steps: taking acrossocheilus fasciatus ova and inactivated opsariichthys bidens sperms for mixed fertilization, adding water to activate the ova, placing the activated ova in ice water, carrying out cold shock treatment for 10-50 minutes, and then placing the ova in normal-temperature water for incubation and seedling emergence. Heterologous sperms and opsariichthys bidens sperms are used as induced sperms, so that the phenomenon that homologous sperms which are not thoroughly inactivated are mixed to form normal diploids is avoided, and complicated verification is eliminated. By inducing gynogenesis, all-female breeding can be achieved, and the breeding yield is remarkably increased. By utilizing a gynogenesis method to perform acrossocheilus fasciatus improved variety breeding, homozygosis can be quickly realized, the breeding time is shortened, and the method has incomparable superiority. The success of inducing the gynogenesis of the acrossocheilus fasciatus is significant for improving the variety of the acrossocheilus fasciatus and increasing the breeding yield, and the problem that a method for inducing the gynogenesis of the acrossocheilus fasciatus by using the heterologous sperms is lacked in the prior art is solved.
Owner:HANGZHOU XIAOSHAN SEA BREEDING CO LTD

Immersion type sperm collecting equipment

The invention relates to the field of sperm collecting equipment, and particularly discloses immersive sperm collecting equipment which comprises a base, and a seat, a sperm collecting mechanism and a multimedia mechanism are arranged on the base; the seat is mounted on the base in a sliding manner; the sperm taking mechanism comprises a first fixing frame, a mechanical arm and a sperm taking barrel, the first fixing frame is fixedly connected with the base, the sperm taking barrel corresponds to the seat, and the two ends of the mechanical arm are rotationally installed on the fixing frame and the sperm taking barrel respectively; the multimedia mechanism comprises a second fixing frame and multimedia equipment, the second fixing frame is fixedly connected with the base, and the multimedia equipment is rotatably installed on the second fixing frame. The device is mainly composed of a seat, a sperm taking mechanism and a multimedia mechanism, and comfortable, convenient and immersive sperm taking experience is provided for a user.
Owner:SANWE MEDICAL TECH JIANGSU

Rapid sperm screening device based on electroosmotic flow and micro-column array manufacturing method

The microfluidic device comprises a shell, a micro-column screening area, a positive electrode, a negative electrode and a cover plate, the positive electrode and the negative electrode are installed on the left side and the right side of the interior of the shell, the micro-column screening area is arranged between the positive electrode and the negative electrode, and the shell is covered with the cover plate; the micro-column screening area is composed of a micro-column array, and micro-channels for sperms to pass through are formed among micro-columns. The invention further discloses a manufacturing method of the micro-column array. The rapid sperm screening device is simple to operate. According to the invention, a micro-nano processing means is adopted to prepare a micro-channel environment for simulating sperm swimming of a microstructure, a high-viscosity buffer solution is adopted to simulate a body fluid environment for sperm swimming, and an electroosmotic flow method is adopted, so that a liquid sample moves from a positive electrode direction to a negative electrode direction, a sperm flowing environment is created in situ, and active sperm screening is accelerated.
Owner:SHANTOU UNIV

Chitosan-pDNA nanoparticle and application thereof in induction of pseudomale Atlantic salmon

The invention discloses chitosan-pDNA nanoparticles and application thereof in induction of pseudo male salmon of Atlantic salmon, and belongs to the technical field of induction of Atlantic salmon strains. The method comprises the following steps: cloning Amh genes of Atlantic salmon to a pIRES2-EGFP vector to obtain plasmids containing the Amh genes; the preparation method comprises the following steps: dissolving chitosan in a 25mM sodium acetate solution according to the concentration of 0.1% (w / v), and adjusting the pH value to 5.0-5.5 by using acetic acid; diluting the concentration of the obtained plasmid to 200ng / mu l to prepare a plasmid solution containing 0.5% (w / v) sodium sulfate; and respectively preheating the two obtained solutions at 55 DEG C, carrying out isovolumetric mixing, carrying out rapid and violent vortex for 55-60 seconds, and standing at room temperature for more than 30 minutes to obtain the chitosan-pDNA nanoparticles. The chitosan-pDNA nano-particles are used for preparing XX type Atlantic salmon pseudo male fish, mature X type sperms are obtained, the X type sperms are fertilized with normal Atlantic salmon X type ova, and Atlantic salmon all-female offspring seeds are obtained.
Owner:OCEAN UNIVERSITY OF CHINA QINGDAO MARITIME SILK ROAD RESEARCH INSTITUTE +1

A method for cryopreservation of sperm of dwarf clams by vitrification

The present invention discloses a method for cryopreservation of sperm of dwarf clams by vitrification, which relates to the field of marine biotechnology. The preservation method comprises the following steps: (1) taking dwarf clams in the breeding period, subjecting them to air-drying and temperature-changing stimulation in sequence to make them naturally discharge sperm, filtering and centrifuging to obtain high-density sperm, and storing it on ice; (2) mixing the high-density sperm obtained in step (1) with a cryoprotectant solution and placing it on ice to obtain a mixed sperm solution; the cryoprotectant solution is composed of dimethyl sulfoxide, coenzyme Q10 and polysucrose; (3) transferring the mixed sperm solution into a cryotube and directly immersing it in liquid nitrogen for storage. It has been found through research that after thawing at a temperature of 50-60 °C for 60-64 s, the D-shaped larva rate reaches more than 80%, realizing a cryopreservation method of directly putting the sample into liquid nitrogen for long-term storage.
Owner:OCEAN UNIV OF CHINA

Medical sperm collector with flexible rolling

The utility model discloses a medical sperm extractor with flexible rolling, which comprises a shell and a flexible colloid arranged in the shell and arranged along the axial direction of the shell, a push cylinder capable of reciprocating along the axial direction of the shell is arranged in the shell, and the flexible colloid is fixedly arranged in the push cylinder along the axial direction of the push cylinder and is linked with the push cylinder. The push cylinder is provided with an opening for exposing the flexible colloid, guide sections are formed on two sides of the opening of the push cylinder, the rolling piece is arranged in the shell, can reciprocate along the axial direction of the flexible colloid and elastically abuts against the flexible colloid, the rolling piece elastically abuts against the flexible colloid at the opening, and the rolling piece is in sliding fit with the guide sections. The sperm collector is reasonable in structural design, the internal structure of the sperm collector is improved, the rolling piece elastically abuts against the flexible rubber body, the flexible rubber body can be continuously clamped, negative emotion of a male during sperm collection is better relieved, and it is guaranteed that the male succeeds in sperm collection.
Owner:WENZHOU ANCONI ELECTRONIC TECH CO LTD

sperm receptacle (1)

ActiveCN309583503SBiotechnologyAnimal science
1. The name of the design product: powder receiving support (1). 2. The use of the design product: for coffee bean grinder powder receiving. 3. The design features of the design product: in shape. 4. The picture or photo that best shows the design features: perspective view.
Owner:GUANGDONG TUNI ELECTRICAL TECH CO LTD

Method for obtaining high-quality fertilized eggs of wild pseudosciaena crocea

The application discloses a method for obtaining high-quality fertilized eggs of wild Pseudosciaena crocea of Lutjanus, and belongs to the technical field of aquatic animal breeding. Wild parent fishes are captured in the breeding season of Pseudosciaena crocea of Lutjanus and are transported to a breeding farm; the wild parent fishes are temporarily raised and intensively cultured, and male and female wild parent fishes with mature gonads are selected, and artificial parturition mixed hormones are injected after anesthesia; after the injection of the artificial parturition mixed hormones, ova and sperm are collected, artificial insemination is performed, and high-quality fertilized eggs are obtained. The technical steps in the application are correlated with each other to form a whole, high-quality fertilized eggs of wild Pseudosciaena crocea of Lutjanus can be successfully obtained, and the proportion of the high-quality fertilized eggs is more than 45%. The high-quality fertilized eggs obtained by the application are used for fry hatching, the hatching rate of the fertilized eggs is more than 62%, and the high-quality fertilized eggs can be used for constructing a basic population of wild Pseudosciaena crocea of Lutjanus, so that artificial breeding of the wild Pseudosciaena crocea of Lutjanus is realized.
Owner:GUANGDONG OCEAN UNIVERSITY

Intracytoplasmic sperm injection (ICSI) support device, intracytoplasmic sperm injection (ICSI) support system, intracytoplasmic sperm injection (ICSI) support program, and egg retention device

To provide an intracytoplasmic sperm injection support device with a simple device configuration. [Solution] Sperm held in sperm injection tool 3 is held by egg holding device An intracytoplasmic sperm injection (ICSI) support device 2 that assists in intracytoplasmic sperm injection (ICSI) in which sperm is injected into an egg, comprising a sperm injection tool 3 and an egg An acquisition unit that acquires information including the relative positional relationship with the child, and the information acquired by the acquisition unit Based on this, an egg retention device is positioned to perform intracytoplasmic sperm injection (ICSI) by injecting sperm into the egg. An intracytoplasmic sperm injection support device 2 comprises a control unit that controls the movement of stage 11.
Owner:ARCS CO LTD

Medical sperm collector with telescopic rotating function

The medical sperm taking device comprises a shell and a flexible rubber body which is arranged in the shell and arranged in the axial direction of the shell, a containing cup is arranged in the shell, the flexible rubber body is installed in the containing cup, a supporting frame connected with the containing cup is arranged in the shell, the supporting frame is fixedly connected with a telescopic rotating shaft, and the telescopic rotating shaft is fixedly connected with the flexible rubber body. The telescopic rotating shaft drives the support frame to rotate and axially reciprocate along the shell; a power source and a motor are arranged in the shell, an output shaft of the motor is connected with a gear shaft, the telescopic rotating shaft is arranged in the gear shaft in a reciprocating motion mode, and the gear shaft drives the telescopic rotating shaft to rotate. The sperm extraction device is reasonable in structural design, and the telescopic rotating shaft is arranged to realize the actions of stretching and rotating the flexible colloid, so that the sperm extraction is smoother when the sperm extraction is performed on the male, and the negative emotion of the male when the sperm extraction is performed can be effectively relieved.
Owner:WENZHOU ANCONI ELECTRONIC TECH CO LTD

sperm guide

ActiveCN309796348SBiotechnologyZoology
1. The name of the design product: powder receiving ring. 2. The use of the design product: for coffee powder receiving. 3. The design points of the design product: in shape. 4. The picture or photo that best shows the design points: design 1 perspective view. 5. Design 1 is designated as the basic design.
Owner:HUIZHOU GUANGYI KITCHEN INTELLIGENT PRODUCTS TECHNOLOGY CO LTD