70 results about "Sperm" patented technology

The present technology relates to a systems for quantifying rheotaxis in a sperm-containing sample. The system includes a microfluidic system having an inlet for charging fluids into a passage. One or more probes defining a confinement region suitable for retaining motile sperm are within the passage. The system further comprises an image processing computing device for obtaining a sequence of images of the confinement region of at least one of the one or more probes having motile sperm retained therein. The sequence of images of the confinement region is processed to determine a signal intensity value for said sequence of images, wherein said signal intensity value is based on a concentration of the motile sperm located in the confinement region at the flow rate. A rheotaxis quality value is determined for said sperm-containing sample based on the signal intensity value. Methods for quantifying rheotaxis in a sperm-containing sample are also disclosed.

The invention relates to the field of distant hybridization breeding, and discloses an artificial breeding method for hybrid species of humpback perches and epinephelus fuscoguttatus, which comprises the following steps: selecting healthy humpback perches as female parent fishes, and screening healthy epinephelus fuscoguttatus as male parent fishes; carrying out intensive cultivation on the parents by means of nutrient enrichment and temperature regulation and control, and promoting synchronous development and maturation of gonads of the parents; after the parents reach the breeding state, eggs of the female parent fishes and seminal fluid of the male parent fishes are collected respectively, fertilized eggs are obtained through artificial insemination, incubation management such as temperature control and continuous oxygenation is conducted on the fertilized eggs, and finally hybrid grouper fries are obtained. The crossbreeding application of the humpback perch as the female parent and the epinephelus fuscoguttatus is realized, and the problems that in the traditional hybridization process, parent gonad development is asynchronous, and filial generations cannot develop are effectively solved; the bred hybrid provides technical guarantee for standardization and precision promotion of grouper crossbreeding, and creates favorable conditions for breeding new hybrid lines with excellent characters of parents.

The invention provides a preparation method and application of a high-activity salmon salmon sperm PDRN (DNA sodium) raw material. The preparation method comprises the following steps: crushing and freezing a salmon testis at an ultralow temperature, adding a mixed solution of a Tris-HCl buffer solution and sodium chloride, and dispersing at a low temperature; adding a compound enzyme preparation with the total enzyme activity of more than or equal to 350KU / L, and carrying out low-temperature appropriate pH enzymolysis; and adjusting the pH value to a suitable range, and carrying out frequency conversion ultrasonic and high-pressure homogenization alternate synergistic treatment in an ice-water bath to obtain the DNA fragmentation liquid, so that the defects of low PDRN activity retention rate, insufficient purity and high protein residue in the prior art are overcome.

The application discloses six boar sperm motility miRNA markers, which are ssc-miR-486, ssc-miR-10386, ssc-miR-708-5p, ssc-miR-122-5p, ssc-miR-199a-3p and ssc-miR-31, wherein the ssc-miR-486, ssc-miR-10386, ssc-miR-708-5p, ssc-miR-122-5p and ssc-miR-199a-3p are highly expressed in low-motility semen sperm plasma exosomes and lowly expressed in high-motility semen sperm plasma exosomes, and the ssc-miR-31 is lowly expressed in low-motility semen sperm plasma exosomes and highly expressed in high-motility semen sperm plasma exosomes. The application has good prospects for identifying boar sperm motility, and the disclosed miRNA markers have accuracy and reliability.

This invention discloses a method for large-scale breeding of all-female triploid mandarin fish, comprising the following steps: (1) Preparation of all-female diploid fertilized eggs: using male mandarin fish pseudo-males (XX) induced by androgens and female mandarin fish (XX) selected from superior strains as parent fish, collecting sperm and eggs, mixing the two, adding water at a temperature of 28℃ to activate sperm for fertilization, and obtaining all-female diploid fertilized eggs; (2) Heat shock induction of triploids: after the all-female diploid fertilized eggs are fertilized in the above-mentioned water at a temperature of 28℃ for 8 minutes, they are immediately transferred to water at a temperature of 39~41℃ for 1~2 minutes for heat shock induction, and all-female triploid fertilized eggs are obtained; (3) Seedling hatching and cultivation: the fertilized eggs induced by heat shock in step (2) are transferred to the hatching system for hatching, and then conventional seedling cultivation is carried out to obtain all-female triploid mandarin fish seedlings. The mandarin fish fry produced by this method have a high female rate, triploid rate, and survival rate.

This invention discloses a method for identifying the parentage between assisted reproductive embryos and sperm or egg donors, belonging to the field of paternity testing technology. The method includes the following steps: S101: Obtaining a single-cell amplification product sample S and sperm / egg donor samples F / M; S102: Sequencing the dimorphic loci of the two samples to obtain DNA data N(S) and N(F / M); S103: Using the loci in the statistical locus set N(S)∩N(F / M) as valid loci; S104: Calculating the mismatch rate and correlation between the two samples based on the valid loci; S105: Determining the parentage between the two samples based on the mismatch rate and correlation; if the correlation ∈ [0.45, 0.60] and the mismatch rate ∈ [0, 3.125%], then the two samples are determined to be parent-child. This patent can accurately determine the parentage between the embryo and the sperm / egg donor even when the genome may be lost.

The present disclosure provides a microscopic insemination support device having a simple configuration. The present disclosure relates to a microscopic insemination support device that supports microscopic insemination in which sperm held by a sperm injection tool is injected into an egg held by an egg holding device. The microscopic insemination support device comprises an acquisition unit configured to acquire information including a relative positional relationship between the sperm injection tool and the egg, and a control unit configured to, on the basis of the information acquired by the acquisition unit, control movement of a stage on which the egg holding device is disposed so as to perform microscopic insemination by injecting sperm into the egg.

The invention relates to a real-time testis rare sperm auxiliary identification method and device which are used for testis incision sperm extraction under a microscope, and the method comprises the following steps: obtaining first real-time image dynamic data; inputting the first real-time image dynamic data to a rare testis sperm auxiliary identification model to obtain rare testis sperm data; and updating the first real-time image dynamic data based on the rare testis sperm data to obtain second real-time image dynamic data. The method has the advantages that due to the fact that the auxiliary identification model for the rare sperms of the testis is high in response speed, the first real-time image dynamic data can be rapidly identified and updated, and the problems of labeling dislocation, repeated identification and the like cannot occur.

This invention discloses a method for extracting high-purity PDRN from salmon testes and its application in improving skin condition, belonging to the field of bioactive ingredient extraction technology. Frozen salmon testis tissue is cleaned of blood vessels and fatty impurities, then cryogenically pulverized at -20~-25℃. The pulverized tissue is added to a pre-treatment dispersion buffer and homogenized under high pressure to obtain a DNA fragmentation solution. The precipitate is then separated by centrifugation. The precipitate is dispersed in a dedicated enzyme digestion buffer, and enzymatic digestion with protease and RNase A is performed to inactivate the enzyme. DNase I is added to the digestion solution for enzymatic cleavage. After the reaction, a stop solution is added to terminate the reaction, and the supernatant is separated by centrifugation. Ethanol is added to the supernatant for precipitation, and centrifugation yields PDRN from salmon testes. This invention, through specific buffer systems and enzymatic digestion conditions, increases the PDRN content by 40~60 kDa, allowing for better absorption and utilization by the skin, improving facial hydration and elasticity, effectively reducing facial wrinkles, and improving facial skin condition. It can be used to prepare functional cosmetics.

The utility model provides an animal sperm injector convenient to use, and relates to the technical field of animal artificial insemination. The animal semen injection device convenient to use comprises a semen injection supporting rod body, a semen injection elastic outer sleeve, an easily-folded leakage-proof piece and a blocking piece. The sperm injection elastic outer sleeve is arranged on the sperm injection supporting rod body in a sleeving mode, the two ends of the sperm injection elastic outer sleeve are connected with the sperm injection supporting rod body in a sealed mode, and a containing cavity used for containing sperm is formed between the sperm injection supporting rod body and the sperm injection elastic outer sleeve; the front end of the sperm injection supporting rod body is provided with a first mounting hole for mounting the easy-to-break leakage-proof piece, and the first mounting hole is communicated with the accommodating cavity; a second mounting hole for mounting the blocking piece is formed in the tail end of the semen injection supporting rod body, and the second mounting hole is communicated with the containing cavity; and temperature control wax is arranged in the easy-to-fold leakage-proof piece. The technical effect that the fertilization device is convenient to use is achieved.

The present application belongs to the technical field of fish breeding, and discloses a method for efficiently inducing and rapidly identifying homologous tetraploid crucian male-nuclear development offspring, comprising the following steps: inducing parturition of female hybrid crucian and male homologous tetraploid crucian, obtaining hybrid crucian ovum, placing the ovum in ovary liquid, inactivating the ovum through ultraviolet lamp irradiation, stirring and uniformly mixing the ovum with sperm of the homologous tetraploid crucian to make the ovum "fertilized", without chromosome doubling treatment, and obtaining survival offspring after hatching and feeding, wherein a pair of oral papillae in the survival offspring is the male-nuclear development offspring. The hybrid crucian ovum selected by the present application contains a heterologous genome itself, has better compatibility, is beneficial to improving the hatching rate, can efficiently induce homologous tetraploid crucian male-nuclear development, and simultaneously utilizes the appearance characteristic of the male-nuclear development individual inheriting a pair of oral papillae from the father to rapidly identify the male-nuclear development offspring, so that the goal of rapidly identifying homologous tetraploid crucian male-nuclear development offspring is achieved, and the present application has important significance in fish genetic breeding.

The invention discloses Lap3a, Lap3b and Lap3c genes of bactrocera cucurbitae and an application of the Lap3a, Lap3b and Lap3c genes. The space-time and tissue expression profiles of the Lap3a, Lap3b and Lap3c genes of the bactrocera cucurbitae show that the three genes are specifically and highly expressed in testis of male bactrocera cucurbitae, especially in transformation regions in testis tissues of adult bactrocera cucurbitae. The Lap3a, Lap3b and Lap3c genes play important functions in the process of participating in maintaining the male reproduction of the bactrocera cucurbitae, when the Lap3a, Lap3b or Lap3c genes of male bactrocera cucurbitae are inhibited, the number of male sperms of the bactrocera cucurbitae is reduced, the reproductive power of the male bactrocera cucurbitae is reduced, the treated male bactrocera cucurbitae is mated with normal female bactrocera cucurbitae, and the egg laying amount and egg hatching rate of the female bactrocera cucurbitae are reduced. The three genes are expected to become SIT potential targets to be applied to pest control, and a new thought is provided for green control of bactrocera cucurbitae.

This invention discloses a highly efficient method for hatching fish adhesive fertilized eggs, specifically including the following steps: Adhesive eggs and sperm are mixed with feathers to form fertilized eggs; 20-40 times their volume of 0.65%-0.9% physiological saline is added, and the mixture is further mixed with feathers to form a fertilized egg mixture; the egg-collecting part is horizontally placed in the hatching tank using a control mechanism, with the egg-collecting part 10-20 cm above the water surface; the fertilized egg mixture is then poured into a funnel, and the funnel is moved back and forth and side to side to ensure the fertilized egg mixture flows evenly onto the egg-collecting part; the egg-collecting part is rotated using a control mechanism to ensure that all positions on the egg-collecting part are covered with fertilized eggs; and finally, the egg-collecting part with the fertilized eggs attached is vertically hung in the hatching tank using a control mechanism. The egg-collecting part of this invention includes a straight rod and multiple sets of filaments. The multiple sets of filaments are distributed vertically along the straight rod to form a columnar structure. The columnar structure has a three-dimensional spatial shape, good air permeability, and can collect eggs in different areas, resulting in a high egg carrying capacity. Furthermore, it prevents the fertilized eggs from piling up and squeezing each other, effectively avoiding cross-infection.

The invention discloses chitosan-pDNA nanoparticles and application thereof in induction of pseudo male salmon of Atlantic salmon, and belongs to the technical field of induction of Atlantic salmon strains. The method comprises the following steps: cloning Amh genes of Atlantic salmon to a pIRES2-EGFP vector to obtain plasmids containing the Amh genes; the preparation method comprises the following steps: dissolving chitosan in a 25mM sodium acetate solution according to the concentration of 0.1% (w / v), and adjusting the pH value to 5.0-5.5 by using acetic acid; diluting the concentration of the obtained plasmid to 200ng / mu l to prepare a plasmid solution containing 0.5% (w / v) sodium sulfate; and respectively preheating the two obtained solutions at 55 DEG C, carrying out isovolumetric mixing, carrying out rapid and violent vortex for 55-60 seconds, and standing at room temperature for more than 30 minutes to obtain the chitosan-pDNA nanoparticles. The chitosan-pDNA nano-particles are used for preparing XX type Atlantic salmon pseudo male fish, mature X type sperms are obtained, the X type sperms are fertilized with normal Atlantic salmon X type ova, and Atlantic salmon all-female offspring seeds are obtained.

The utility model discloses a medical sperm extractor with flexible rolling, which comprises a shell and a flexible colloid arranged in the shell and arranged along the axial direction of the shell, a push cylinder capable of reciprocating along the axial direction of the shell is arranged in the shell, and the flexible colloid is fixedly arranged in the push cylinder along the axial direction of the push cylinder and is linked with the push cylinder. The push cylinder is provided with an opening for exposing the flexible colloid, guide sections are formed on two sides of the opening of the push cylinder, the rolling piece is arranged in the shell, can reciprocate along the axial direction of the flexible colloid and elastically abuts against the flexible colloid, the rolling piece elastically abuts against the flexible colloid at the opening, and the rolling piece is in sliding fit with the guide sections. The sperm collector is reasonable in structural design, the internal structure of the sperm collector is improved, the rolling piece elastically abuts against the flexible rubber body, the flexible rubber body can be continuously clamped, negative emotion of a male during sperm collection is better relieved, and it is guaranteed that the male succeeds in sperm collection.

1. The name of the design product: powder receiving support (1). 2. The use of the design product: for coffee bean grinder powder receiving. 3. The design features of the design product: in shape. 4. The picture or photo that best shows the design features: perspective view.

The application discloses a method for obtaining high-quality fertilized eggs of wild Pseudosciaena crocea of Lutjanus, and belongs to the technical field of aquatic animal breeding. Wild parent fishes are captured in the breeding season of Pseudosciaena crocea of Lutjanus and are transported to a breeding farm; the wild parent fishes are temporarily raised and intensively cultured, and male and female wild parent fishes with mature gonads are selected, and artificial parturition mixed hormones are injected after anesthesia; after the injection of the artificial parturition mixed hormones, ova and sperm are collected, artificial insemination is performed, and high-quality fertilized eggs are obtained. The technical steps in the application are correlated with each other to form a whole, high-quality fertilized eggs of wild Pseudosciaena crocea of Lutjanus can be successfully obtained, and the proportion of the high-quality fertilized eggs is more than 45%. The high-quality fertilized eggs obtained by the application are used for fry hatching, the hatching rate of the fertilized eggs is more than 62%, and the high-quality fertilized eggs can be used for constructing a basic population of wild Pseudosciaena crocea of Lutjanus, so that artificial breeding of the wild Pseudosciaena crocea of Lutjanus is realized.

To provide an intracytoplasmic sperm injection support device with a simple device configuration. [Solution] Sperm held in sperm injection tool 3 is held by egg holding device An intracytoplasmic sperm injection (ICSI) support device 2 that assists in intracytoplasmic sperm injection (ICSI) in which sperm is injected into an egg, comprising a sperm injection tool 3 and an egg An acquisition unit that acquires information including the relative positional relationship with the child, and the information acquired by the acquisition unit Based on this, an egg retention device is positioned to perform intracytoplasmic sperm injection (ICSI) by injecting sperm into the egg. An intracytoplasmic sperm injection support device 2 comprises a control unit that controls the movement of stage 11.

1. The name of the design product: powder receiving ring. 2. The use of the design product: for coffee powder receiving. 3. The design points of the design product: in shape. 4. The picture or photo that best shows the design points: design 1 perspective view. 5. Design 1 is designated as the basic design.

The invention provides a preparation method of a high-activity salmon salmon sperm polynucleotide PN (DNA sodium) extraction raw material and application of the high-activity salmon salmon sperm polynucleotide PN (DNA sodium) extraction raw material. Frozen salmon salmon sperm tissue, pretreated anabaena variabilis and fermented sake lactobacillus are used as ternary composite raw materials, mixed according to a specific proportion, subjected to impurity removal and then subjected to ultralow-temperature crushing, a composite buffer solution and composite salt are added, and the high-activity salmon salmon sperm polynucleotide PN (DNA sodium) extraction raw material is obtained. Performing ultrasonic-assisted stirring and low-temperature dispersion; then carrying out segmented enzymolysis at gradient pH and proper temperature by adopting a pH response type immobilized double-enzyme system, and automatically controlling the pH; the enzymatic hydrolysate is subjected to low-temperature multi-field coupling synergistic fragmentation to realize efficient release and homogenization of DNA, and then is subjected to low-temperature centrifugation, multistage membrane separation, specific capture and refining decoloration to finish graded purification; and finally adding a composite low-temperature protective agent and hyaluronic acid derivative microspheroidizing modification, and carrying out gradient heating and freeze-drying to obtain the product. The cyclic utilization rate of the enzymatic hydrolysate is greater than or equal to 75%, and the method is environment-friendly and efficient.

The invention discloses a method for inducing gynogenesis of Sinilabeo notabilis by white crucian carp sperms and application thereof, and the method for inducing gynogenesis of Sinilabeo notabilis by white crucian carp sperms comprises the following steps: (1) collecting and diluting white crucian carp sperms, and then carrying out inactivation treatment by ultraviolet lamp irradiation; (2) mixing the inactivated seminal fluid of the carassius auratus and the inactivated mature eggs of the sinilabeo nolatus, performing dry insemination, then pouring the mixture into cold water for treatment, putting fertilized eggs into a normal-temperature water incubation tank for incubation after cold treatment is finished, and keeping running water to enable the fertilized eggs to float along with water flow and not to sink to the bottom; and (3) after fry are hatched from the fertilized eggs, culturing to obtain gynogenetic sinilabeo nolabeo. According to the method, the carassius auratus sperms are adopted to induce the gynogenesis of the semilabeo nolabeo to successfully breed and obtain the gynogenesis semilabeo nolabeo offspring, which is the first time in the artificial induction gynogenesis and germplasm improvement of the semilabeo nolabeo. In the breeding process, it is found that the 6-month-old semilabeo notabilis has the advantages that the growth speed is high, and the low-temperature resistance is remarkably higher than that of the parent semilabeo notabilis.

The application discloses a sperm extractor, which comprises a main body, a first stimulating part and a driving assembly. The main body is formed with a sperm extraction space and a mounting space which is arranged at intervals with the sperm extraction space; the first stimulating part is arranged in the sperm extraction space, and a stimulating cavity is formed in the first stimulating part; the driving assembly is arranged in the mounting space; the driving assembly comprises a driving motor, a first transmission part which is fixedly matched with the first stimulating part, a second transmission part which is slidingly matched with the first transmission part, a first transmission unit which comprises a worm wheel and a worm, a second transmission unit which is connected between the worm wheel and the first transmission part, and a third transmission unit which is connected between the worm wheel and the second transmission part. The worm is connected with the driving motor, the second transmission unit converts the rotating motion of the worm wheel into the linear motion of the first transmission part along a first axis, and the third transmission unit comprises two gears which are used for driving the second transmission part to rotate around the first axis. The sperm extractor has the advantages of compact structure, composite stimulating effect and improved sperm extraction efficiency.

This invention discloses a method for preparing sodium DNA from salmon sperm and its application, belonging to the field of biomedical technology. The preparation method includes the following steps: S1. Raw material pretreatment: Frozen salmon or salmon testis tissue is lysed at -30 to -20℃, then a mixed solution containing Tris-HCl buffer, NaOH, and NaCl is added, followed by the addition of a chitosan-embedded complex enzyme with a total enzyme activity ≥350KU / L. The enzyme is then hydrolyzed at 37±1℃ for 26-30 hours, maintaining a pH of 7.8-8.0 to obtain the enzymatic hydrolysate; S2. DNA synergistic lysis: The pH of the enzymatic hydrolysate is adjusted to 7.8, and the mixture is synergistically treated in an ice-water bath at ≤10℃ by alternating cycles of 25-35kHz variable frequency ultrasound and 100-120MPa high-pressure homogenization for 40 minutes to obtain the DNA lysate. This method solves the problems of high protein residue, uneven molecular weight distribution, low transdermal absorption, high cost, and poor environmental friendliness in traditional extraction processes, and has the advantages of high product purity, strong activity, low cost, and green environmental protection.

The application discloses an artificial breeding method of Epinephelus fuscoguttatus and Plectropomus leopardus hybrids, which comprises the following steps: screening healthy Epinephelus fuscoguttatus as female parents, screening healthy Plectropomus leopardus as male parents, intensively breeding the male and female parents, the intensive breeding comprising nutrition intensification and / or temperature induction to promote gonadal maturation of the parents; collecting egg liquid of the female parents and sperm of the male parents, fertilizing to obtain fertilized eggs, incubating the fertilized eggs to obtain hybrids, and using one or more sequences in sequences SEQ ID NO. 1-8 to identify the authenticity of the hybrids, so as to determine that the hybrids inherit the genetic information of the female and male parents. The application fills the technical blank of using Plectropomus leopardus as a parent Epinephelus to carry out hybrid breeding, and makes the authenticity of the hybrids obtained by hybridization of Epinephelus fuscoguttatus and Plectropomus leopardus reliable, and the hybrids inherit the genetic information of the parents at the molecular level.

The invention relates to a method for increasing the lifespan of animal sperm comprising contacting said sperm with an inhibitor of Slo3 potassium channel.The invention also relates to a use of an inhibitor of Slo3 potassium channel, for increasing the lifespan of animal sperm or motility of capacitated animal sperm, comprising contacting an inhibitor of Slo3 potassium channel with said sperm. Moreover, the invention relates to an artificial insemination instrument for use in artificial insemination of an animal, comprising animal sperm in contact with an inhibitor of Slo3 potassium channel.The invention also relates to a method for artificially inseminating an animal using said artificial insemination instrument.Eventually, the invention relates to a method for increasing the fertility of an animal, comprising contacting sperm of said animal with an inhibitor of Slo3 potassium channel; then artificially inseminating said animal with said sperm.

This invention discloses a compound moxa stick for warming yang and unblocking obstruction, its preparation method, and its application in improving reproductive system function. It belongs to the field of external preparations of traditional Chinese medicine. Addressing the problems of limited improvement effects of pure moxa sticks, lack of systematic design in compound moxa stick formulations, unclear synergistic effects between components, large fluctuations in conditioning effects, and difficulty in balancing conditioning effects and safety, this invention uses specific weight proportions of Artemisia argyi, Cyperus rotundus, Alpinia galanga, Cinnamomum cassia, Zingiber officinale, Illicium verum, Cinnamomum cassia twig, Spatholobus suberectus, Caesalpinia sappan, Smilax glabra, Euonymus fortunei, Citrus aurantium, Amomum villosum, Citrus reticulata peel, and Atractylodes lancea to prepare a compound moxa stick. The components synergistically enhance each other, and this product is used to prepare a moxibustion intervention product for improving the reproductive function of mammals. It can stably improve female ovarian reserve function and male asthenospermia, simultaneously regulate reproductive-related hormone levels, repair pathological damage to reproductive organs, and improve the quality of reproductive cells.

The invention provides a method for increasing the hatching rate of fertilized eggs of Epinephelus yunnanensis, and belongs to the technical field of fish culture. The method comprises the following steps: S1, parent cultivation and male transformation: selecting five-year-old Epinephelus lanceolatus without seminal fluid as a male parent to perform indoor circulating water cultivation, and inducing male transformation in a cooperative mode of feeding a feed containing methyltestosterone, injecting follicle-stimulating hormone and periodically increasing and decreasing water temperature in the cultivation process; s2, male fish screening: regularly checking the male conversion rate in the parents in the step S1, screening the male fishes with qualified maturity, and screening the male fishes with qualified sperm maturity from the male fishes with qualified maturity; s3, insemination and incubation: collecting the seminal fluid of the male fishes screened in the step S2 for artificial insemination of the eggs of the epinephelus moara, and incubating the fertilized eggs. According to the method, the problems of small male fish quantity, insufficient seminal fluid and low fertilized egg hatching rate in artificial breeding of groupers are solved.

A method for human sperm vitrification is disclosed, which includes providing liquefied human sperm; having a vitrification medium, where the vitrification medium has (a) a sperm buffer, and (b) a cryoprotectant mixture including a permeable cryoprotectant and a non-permeable cryoprotectant; mixing the liquefied sperm with the vitrification medium; providing a sperm straw and loading the straw with 0.25 to 0.5 mL of the obtained mixture; sealing the straw with heat; and placing the straw vertically in a container with liquid nitrogen to vitrify the mixture. A portable kit is also disclosed, which makes it easy to implement the method. The kit includes a vitrification medium, some straws, a container, usage instructions, among others.

The application discloses a method for inducing tetraploid of Haliotis discus hannai Ino, and relates to a method for cultivating tetraploid of shellfishes, which comprises the following steps: 1) selecting and accelerating maturation of seed abalones: selecting healthy and active male and female seed abalones without external damage, and placing the seed abalones in a seed abalone cultivation pool to accelerate maturation simultaneously; 2) artificially inducing spawning: selecting diploid abalones with well-developed gonads to induce spawning, and stopping until the male and female seed abalones spawn and ejaculate; 3) selecting and mixedly fertilizing sperm and eggs: filtering impurities in sperm and eggs respectively, and synchronously mixedly fertilizing the sperm and the eggs by adding the sperm into the egg liquid; 4) cold shock induction: when 60% to 80% of the fertilized eggs are ready to start the first cleavage and elongation, the fertilized eggs are immediately collected into pre-cooled seawater with a temperature of 2-8 DEG C to be treated for 10-15 min, so as to inhibit the first cleavage; and 5) incubation: immediately placing the fertilized eggs into an incubation water tank with a temperature of 18-22 DEG C to be incubated after the cold shock is finished. The method for inducing tetraploid of Haliotis discus hannai Ino realizes efficient and stable obtaining of tetraploid of Haliotis discus hannai Ino by improving the method for inducing spawning of seed abalones and optimizing the inducing conditions.

The invention relates to the technical field of sperm enrichment, in particular to a method for separating and enriching sperms by adopting IgY antibody immunomagnetic beads, which comprises the steps of sample carrier separation and sperm enrichment, and is characterized in that the sample carrier separation comprises the steps of digestion, low-position centrifugation and high-position centrifugation, and the sperm enrichment comprises the steps of immunomagnetic bead combination, magnetic separation and cleaning. The immunomagnetic bead coupled with the IgY antibody has the characteristics of strong specificity, high temperature resistance and acid and alkali resistance, belongs to a food-grade safety reagent, and is low in production cost, complete DNA can be detected only by injecting the immunomagnetic bead coupled with the IgY antibody for immunization of a hen for multiple times, separating and purifying the antibody after regular egg taking, wrapping the immunomagnetic bead coupled with the IgY antibody and tightening sperms, and 30 sperms. Compared with the prior art, an obvious experiment effect is achieved, laboratory operation is more convenient, the immunomagnetic beads can be combined with an instrument to achieve automatic operation, and sperm enrichment operation of mixed spot detection materials is greatly simplified.