39 results about "Pomfret" patented technology

The invention provides an AI-based trachinotus ovatus juvenile fish culture water parasite prediction and prevention method and system, and the method comprises the steps: collecting multi-source data from a culture water body through a sensor array, including a nucleic acid signal, a fish school behavior track and an environment parameter sequence, and obtaining an original data set; unifying the data format and the acquisition frequency according to the original data set by adopting a time sequence alignment method, and obtaining an aligned feature flow; aiming at the aligned feature flow, applying a convolutional neural network to extract a space-time pattern of a nucleic acid signal and an abnormal index of a fish school behavior, and determining an extracted feature set; if the abnormal index in the extracted feature set exceeds a preset threshold value, a causal association between environmental parameters and parasitic risks is classified through a support vector machine, and a preliminary risk level is judged; simulating a water body change scene according to the comprehensive causal model to obtain a predicted trajectory of parasite propagation; and determining a quantitative score of the parasite risk and generating a prevention and control instruction sequence by comparing the predicted trajectory with the real-time monitoring data.

The invention belongs to the technical field of biology, and particularly discloses a high-temperature-resistant antibacterial protein Cystatin B sourced from marine fish as well as a preparation method and application of the high-temperature-resistant antibacterial protein Cystatin B. The high-temperature-resistant antibacterial protein Cystatin B is derived from pomfret, and the amino acid sequence of the high-temperature-resistant antibacterial protein Cystatin B is as shown in SEQ ID NO. 1; the nucleotide sequence of the gene for coding the high-temperature-resistant antibacterial protein Cystatin B is as shown in SEQ ID NO.2, and the sequences of primers adopted for cloning the gene are as shown in SEQ ID NO.3-4. The high-temperature-resistant antibacterial protein Cystatin B has a remarkable antibacterial effect on gram-negative bacteria such as escherichia coli, vibrio parahaemolyticus and edwardsiella tarda as well as gram-positive bacteria such as staphylococcus aureus; and after being treated at the temperature of 100 DEG C, the antibacterial agent still can keep relatively high antibacterial activity. The antibacterial protein is simple in preparation method, can replace traditional antibiotics, and can quickly and efficiently prevent and treat various common bacterial diseases in aquaculture.

The invention relates to the field of trachinotus ovatus swarm sound production identification, and discloses a method for identifying sound production of a trachinotus ovatus swarm in a net cage based on dual-channel time-varying Wiener filtering. According to the method, modeling and target signal counteracting are respectively carried out according to the conditions of the left sound channel and the right sound channel of the input signal so as to analyze the noise signal-to-noise ratio characteristics, enhancement of target biological sound and suppression of noise signals are realized in combination with a Wiener filtering method, trachinotus ovatus sound production can be identified in a complex noise environment, and the method has the target positioning capability. According to the method, by introducing a dual-channel signal equalization and interference compensation mechanism and fusing posterior and prior signal-to-noise ratio estimation, the sound production of the trachinotus ovatus swarm can be effectively identified under the complex noise condition without training data, the problems that the performance is poor and a large amount of training data is needed when non-stationary noise is separated by a traditional noise separation method are solved, and the method is suitable for popularization and application. Technical prerequisites are provided for net cage acoustic monitoring work, and possibility is created for rapid, efficient and accurate sound production analysis of subsequent net cage fish schools.

The invention belongs to the technical field of aquaculture intelligent detection, and discloses a golden pomfret malformation and injury automatic detection method based on deep learning. According to the method, a complete data set containing normal fishes, deformed fishes and injured fishes is constructed, a fin removal labeling strategy is adopted, and an optimized YOLO11 model is combined, so that synchronous detection of the malformation and the injury of the trachinotus ovatus is realized; and (2) aiming at the optimization of the small target defect and sample imbalance problem, the tiny damage detection capability is improved: a Focal Loss loss function and a 160 * 160 high-resolution segmentation head are introduced on a basic YOLO11 structure, and key optimization of small target damage and minority class defect samples is realized. The Focal Loss dynamically adjusts the weight of the sample, so that the model pays more attention to difficult-to-detect tiny damage samples and few damage samples in training; the newly-added high-resolution segmentation head can retain more shallow detail features, and the detection capability of tiny damage on the surface of the fish body is remarkably enhanced.

The invention discloses a culture method based on posthypoxia gastric intima separation treatment of freshwater white pomfret, and relates to the technical field of aquaculture and disease prevention and control. The method comprises the following steps: step 1, identifying hypoxia symptoms; 2, medicine compatibility feeding is carried out; 3, regulating and controlling the water body environment; 4, breeding environment management; and step 5, continuous monitoring. According to the invention, the food maternal, the endothelium corneum gigeriae galli and the pipemidic acid are combined for the separation treatment of the endogastric membranes of the aquatic animals for the first time, the thinking limitation of traditional disease prevention and treatment is broken through, the traditional Chinese and western medicine theories are organically combined, and a new disease prevention and treatment thought is provided for the aquaculture industry; compared with a traditional pure antibiotic treatment or water quality improvement method, the scheme is high in pertinence and directly aims at a core pathological link of gastric intima separation after hypoxia; the comprehensive treatment effect is good, and meanwhile, anti-infection, mucous membrane repair and function regulation are considered; and the used medicines are all common medicines, have small side effects and low residues, and meet the safety requirements of aquatic products.

The invention belongs to the technical field of aquaculture, and particularly relates to a golden pomfret sectional type culture method. According to the sectional type breeding method, nutrition is accurately supplied according to the requirements of the trachinotus ovatus in different growth stages, and the growth speed is remarkably increased; the growth speed of the trachinotus ovatus is obviously increased by optimizing the water temperature and illumination conditions in stages, so that the culture cycle is shortened; the licorice powder and the melatonin are added into the feed, so that the immunity of the trachinotus ovatus is further enhanced, and the use of medicines is reduced. According to the embodiment of the invention, the unit yield and the survival rate of the trachinotus ovatus are improved through sectional culture, and the economic benefit is remarkably improved.

The invention relates to a preparation method of a complete puffed compound feed for trachinotus ovatus. Belongs to the technical field of expanded feed for marine fish. The pregelatinized starch is used for lowering the temperature of the puffing and tempering section, and the water vapor is used for adjusting the initial water content to 19-23%, so that the high-temperature inactivation is reduced; the passing time of the screw section is controlled, and mechanical shearing and heat accumulation are reduced; the instant high-temperature pressure of the puffing section is suddenly reduced to form a porous structure, and the dissolution rate control of crude protein and crude fat is promoted by matching with the proper proportion of the pre-gelatinized corn starch and the cassava starch and the tackifying effect of the poultry eggs; probiotics and metabolites thereof in the fermentation liquor are reserved to the maximum extent; the viable count of probiotics in the puffed granules is remarkably increased and is far higher than that of conventional high-temperature granulation; butyric acid, lactic acid, antibacterial peptide and the like generated by fermentation are blended into the feed, so that growth of pathogenic bacteria is inhibited, and intestinal health of the trachinotus ovatus is enhanced.

The invention belongs to the technical field of disease prevention and control in aquaculture, and discloses application of arecoline derivatives in killing Bowden worms. A coarse screening experiment, a soaking toxicity test and a dose effect experiment prove that in coarse screening, 0.5 mg / L of the arecoline derivative is soaked for 90 min, so that the killing rate of the arecoline derivative on the bodworm reaches 75%, and the arecoline derivative is obviously superior to the other five kinds of arecoline and derivatives thereof such as triclabendazole and arecoline; a soaking toxicity test shows that after the arecoline derivative with the concentration of 0.7 mg / L is soaked for 90 min, the survival rate of the trachinotus ovatus is 100%, and no remarkable toxicity exists; a dose effect experiment further proves that the killing rate of 0.7 mg / L of the arecoline derivative on the boxworm is increased to 95.35%. The arecoline derivative has a remarkable effect of killing the boxworm, is high in safety to host fishes, has no obvious residual risk, can be further researched and developed to be special for preventing and controlling the boxworm in aquaculture, provides an effective scheme for solving culture diseases caused by the boxworm, and has a wide application prospect.

A quick-freezing method for Trachinotus ovatus. The method comprises the following steps: putting Trachinotus ovatus into a liquid nitrogen flash freezer, the cavity temperature of which is A, and whe

The invention belongs to the technical field of aquaculture intelligent detection, and discloses a trachinotus ovatus weight prediction method. An original image of the trachinotus ovatus is obtained through target design and image acquisition, and accurate segmentation of a trachinotus ovatus body is realized by adjusting a YOLOv11 model. A Shii-Tomasi corner detection algorithm is adopted to extract center coordinates of a target, a perspective transformation technology is utilized to correct an image, and a proportional relation between a pixel size and an actual physical size is established. And inputting the corrected fish body image into the adjusted YOLOv11 model, performing segmentation to obtain a fish body contour coordinate point set, measuring the length and the width of the fish body through a minimum bounding rectangle method, and calculating key parameters such as the perimeter and the area of the fish body contour. Based on the obtained size parameters of the trachinotus ovatus body, a regression model between the size parameters and the weight is established, the adjusted R2 serves as an optimization index, nonlinear fitting and iterative parameter solving are conducted through 1stOpt software, a quality prediction model is established, and non-contact prediction of the trachinotus ovatus weight is achieved.

The invention belongs to the technical field of pomfret breeding, and particularly relates to wet compound feed for breeding juvenile pomfret and a preparation method, a feeding method and a device of the wet compound feed. The wet compound feed for breeding the juvenile pomfret is obtained by mixing powdery feed for the juvenile pomfret and water according to the mass ratio of 1: (1.2-1.5). The powdery feed for the juvenile pomfret is prepared from the following raw materials in parts by weight: 40-50 parts of white fish meal, 8-11 parts of mussel meat powder, 3-6 parts of enzymolysis squid paste, 6-9 parts of yolk powder, 4-8 parts of wheat protein powder, 10-17 parts of puffed corn flour, 4-7 parts of anchovy oil, 2-5 parts of schizochytrium limacinum powder, 0.4-1.0 part of a compound adhesive, 0.2-0.5 part of a compound enzyme preparation and 0.2-0.5 part of a compound microecological preparation. The feed is prepared from the following raw materials in parts by weight: 1.0-2.5 parts of a macroelement premix, 0.8-1.5 parts of a microelement premix and 0.4-1.0 part of a vitamin premix. The feed provided by the invention can solve the problems of low feeding rate, low survival rate and slow growth speed of the existing feed for feeding the pomfret in the prior art.

The present application discloses a device and a production process for puffed compound feed for golden pomfret, relating to the field of aquaculture technology and improving the problem of low processing efficiency. The device comprises an extruder and a discharging device, wherein the extruder is provided with a discharging port, the discharging device comprises a screening seat, a leveling seat, and a collecting seat, the screening seat is provided with a separation device for separating debris, a connecting pipe is provided between the screening seat and the leveling seat, the connecting pipe is provided with a vibration plate, the leveling seat comprises a conveying pipe, a leveling member slides on the conveying pipe, a cooling plate is rotatably connected to the leveling seat, the leveling seat is provided with a cooling assembly, the leveling member is provided with several push plates, and the conveying pipe is provided with a moving assembly; the discharging device is also provided with a control assembly that simultaneously controls the shaking of the vibration plate, the sliding of the leveling member, and the rotation of the cooling plate. The present application can improve processing efficiency.

The invention belongs to the field of food processing, and particularly relates to a method for improving the taste and flavor of pickled trachinotus ovatus. The method comprises the following steps: killing trachinotus ovatus in an ice bath, removing viscera and gills, cleaning, uniformly smearing table salt on the inner and outer surfaces of the trachinotus ovatus, placing the trachinotus ovatus in a high-voltage electrostatic field refrigerator for pickling, cleaning the pickled trachinotus ovatus, and drying to obtain the flavored trachinotus ovatus. Wherein the drying modes comprise heat pump drying, hot air drying, natural airing and combined drying, the natural airing and drying can remarkably shorten the transverse relaxation time of the trachinotus ovatus which is not easy to flow water, and the color and the elasticity are improved; the hot air drying endows the fish meat with better hardness, chewiness and rich volatile flavor; the heat pump drying is fewest in volatile substances and optimal in microbial safety, and the improvement method provided by the invention can improve the elasticity, flavor complexity and microbial safety of the salted golden pomfret, and is beneficial to production of salt-reduced half-dry salted fish products with excellent quality.

The application provides a kind of gold pompano heat stress resistance cultivation method, using domestication pool I, II, III, according to juvenile stage, juvenile stage, adult fish stage, gold pompano is domesticated, in juvenile stage, using bait domestication, through the specific bait formula in nutrition or pharmacological means, the fat utilization ability of fish body can improve the heat stress tolerance of fish, temperature domestication is carried out in juvenile stage, four-stage temperature domestication is adopted, the heat tolerance of fish body is gradually trained, the defense ability of non-specific immunity of fish body is improved, sound wave domestication is carried out in adult fish stage, the sound information is transmitted to the motor-related neuron in hindbrain, so as to promote the tail movement of itself, improve the immune function of fish body, the application domesticates the feeding, enemy, circadian rhythm regulation ability of fish body, improves the physical fitness of fish body to enhance the heat reaction ability, reduces the probability of heat reaction, at the same time, can better, faster adapt to harsh breeding environment, the survival rate reaches 98.8% after stress test, and the weight loss rate is-0.02%.

The utility model provides a kind of fish egg sorting device. When needing to sort gold pomfret fish egg, first, the fish egg is put into the box, then the first valve is opened, and the live egg in the box rises with the water level. When the water level is higher than the first end of the live egg conveying pipe, the live egg floating on the water surface will follow the water into the first end of the live egg conveying pipe, and then be conveyed to the live egg collecting box through the live egg conveying pipe. Finally, the water pump is opened, and the dead egg at the bottom of the box is conveyed to the dead egg collecting box through the dead egg conveying pipe. This application not only has high efficiency compared to manual sorting, but also can effectively save a lot of manpower.

The invention provides an efficient breeding method suitable for trachinotus ovatus, which comprises the following steps: after 8-10cm fries are bred in circulating water, transferring the fries to a deepwater net cage for breeding. In the breeding process, artificial feed containing fish meal, alginic acid, astaxanthin microcapsules and other components in different proportions is accurately fed in the larva fish stage, the juvenile fish stage and the juvenile fish stage, and the feeding amount is adjusted according to the growth stage. Meanwhile, water quality indexes such as salinity and ammonia nitrogen of the breeding environment are controlled, and acousto-optic domestication of 800-1200 Hz sound waves and 470 nm blue LED light is conducted regularly every day. According to the method, fry pretreatment, precise nutrition supply and environment regulation and control are combined, the survival rate and growth speed of the trachinotus ovatus are effectively increased, the feed coefficient is reduced, the meat quality is improved, diseases are reduced, and an innovative scheme is provided for large-scale efficient breeding of the trachinotus ovatus.

The present invention provides a method for improving the water retention of golden pomfret. The method first bleeds unconscious fresh golden pomfret for 4 to 10 minutes, then soaks the fish in a polyphenol solution at a concentration of 100 to 200 mg / L for 8 to 12 minutes. Specifically, by combining two main processes—specific bleeding (blending during coma) and soaking in a polyphenol solution—and by specifically setting process parameters such as the bleeding time, polyphenol solution concentration, and soaking time, the process complements each other, synergistically improving the water retention of the golden pomfret and allowing it to maintain high edible quality even after prolonged frozen storage and transportation.

The invention provides a pomfret-sourced protein with a jellyfish toxin inhibiting effect, which can inhibit cell apoptosis generated by jellyfish toxin phospholipase stimulation, and comprises a complete nucleic acid sequence and an amino acid sequence. The obtained protein can play a role in detoxifying when juvenile fishes eat jellyfish in the breeding process of the pomfret, the effect of inhibiting apoptosis generated by jellyfish toxin stimulation is verified on the cell level through gene overexpression and infection, and the effect is remarkable. The pomfret-sourced protein can be used for producing a jellyfish toxin detoxification preparation, the jellyfish toxin poisoning phenomenon caused by the fact that fish fries eat jellyfish is reduced, the survival rate of the fish fries is increased, and economic benefits are increased for farmers.

The application provides a method for breeding golden pompano, which comprises the following steps: setting a net cage, selecting and releasing fry, bait enhancement and daily management, selecting a suitable sea area as the breeding environment of the net cage according to the living habit of the golden pompano, ensuring a healthy breeding environment, enhancing the bait according to three stages of the golden pompano with different ages, and adjusting the bait according to the growth of the fish body in each stage, so that the fish body reaches a micro-ecological balance in nutrition, immunity, growth stimulation and biological antagonism, and the reproduction of bacterial enteritis is inhibited; the fry is irradiated by visible light with a wavelength of 597-3400 nm while the net cage is cleaned, which can promote the release of cytokines on the surface of the fish body, prevent the infection of vibrio campbellii, tropical pseudomonas aeruginosa and gram-negative bacilli, and the survival rate of the method for breeding the golden pompano is 96.5-98.1%, the incidence of bacterial colitis is 0.3-1.2%, and the incidence of furuncle disease is 0.2-0.9%.

The utility model discloses a fertilized egg hatching device of trachinotus ovatus, which relates to the technical field of aquaculture and comprises a double-layer cylinder body, the double-layer cylinder body comprises an outer-layer peripheral wall and an inner-layer peripheral wall, the inner-layer peripheral wall extends along the axial direction and surrounds to form a conical cylinder body, and the small-caliber end of the conical cylinder body is arranged downwards; a hatching cavity is formed between the inner side surfaces of the inner-layer peripheral wall; the top end of the outer-layer peripheral wall is higher than that of the inner-layer peripheral wall, and an overflow space is formed between the outer-layer peripheral wall and the inner-layer peripheral wall; an overflow pipeline is arranged on the peripheral wall of the outer layer; the water inlet assembly comprises a water inlet pipeline and a water inlet switch; the water inlet pipeline is communicated with a water inlet at the small-caliber end of the conical barrel; and a water inlet switch is arranged on the water inlet pipeline. According to the technical scheme, through the arrangement of the double-layer barrel and the water inlet assembly, incubation liquid in the incubation cavity can be continuously updated, the concentration of harmful substances in water can be reduced, a good water quality environment can be maintained, and favorable conditions are created for the development of fertilized eggs.

A quick-freezing method for Trachinotus ovatus. The method comprises the following steps: putting Trachinotus ovatus into a liquid nitrogen flash freezer, the cavity temperature of which is A, and whe

The invention belongs to the field of multi-nutrition-level polyculture ponds, and particularly relates to a trachinotus ovatus-penaeus monodon-blue crab multi-nutrition-level polyculture pond which comprises a pond body, a lower-layer separation net is slidably connected to the lower middle portion of the inner wall of the pond body, and an upper-layer separation net is slidably connected to the upper middle portion of the inner wall of the pond body. The lower end of the upper-layer separation net is fixedly connected with a plurality of evenly-distributed supporting legs, and the upper end of the upper-layer separation net is fixedly connected with a supporting rod. The upper-layer separation net, the lower-layer separation net, the dismounting and mounting mechanism and other structures are additionally arranged in the pond body, in the using process, the pond body can be divided into three layers for breeding through the upper-layer separation net and the lower-layer separation net, independent species can be separated, mutual predation among bred objects can be effectively avoided, and the breeding efficiency is improved. In addition, in the breeding process, the bottom of the pond body can be oxygenated through an air conveying pipe, a rotary oxygenation frame and other structures at the bottom, and oxygenation of the water body in the pond body can be more uniform.

This invention relates to a red tide early warning net cage for pomfret farming, comprising a net cage, a waterproof cover, support rods, and a control center. The net cage contains a water quality monitoring module and side pipes. The net cage is connected to floats 1 and 2 via support rods 1 and 2. Electric rotating rings 1 and 2 are mounted on support rods 1 and 2, respectively. The two ends of the support rods are respectively connected to support rods 1 and 2. The support rods are connected to the waterproof cover at intervals according to their radius from largest to smallest, allowing the support rods to be distributed in a descending radius order to open the waterproof cover and cover the net cage. The control center can send red tide early warning signals to the user terminal, control the isolation of the net cage, supply oxygen, and treat the aquaculture water based on the water quality parameters monitored by the water quality monitoring module. This invention can not only predict the occurrence and arrival of red tides and provide early warning information to aquaculture personnel, but also selectively isolate the aquaculture water inside the net cage from the outside and treat the internal aquaculture water, reducing the impact of red tides on pomfret.

The invention discloses an out-of-season breeding method for trachinotus ovatus, and relates to the technical field of aquaculture. The method comprises the following steps: selecting a parent for out-of-season breeding of the trachinotus ovatus, performing ovary strengthening on the female parent by utilizing a strengthening agent, performing low-temperature temperature accumulation on the parent, performing fin cutting treatment on the parent, regulating and controlling the gonad development state of the parent by utilizing hormone, performing fertilization, obtaining a fertilized egg, hatching the fertilized egg and the like. According to the method, the parent fish is subjected to strengthening, low-temperature temperature accumulation, fin cutting and other treatment, the gonad development state of the parent fish is accurately regulated and controlled, out-of-season breeding of the trachinotus ovatus is achieved, the breeding efficiency and fry quality of the trachinotus ovatus are improved, the problem that existing trachinotus ovatus breeding is limited by seasons is solved, and good economic benefits and application prospects are achieved.

The application discloses a kind of gold pompano reverse season breeding method, it is related to aquaculture technical field.The method includes selecting the parent for gold pompano reverse season breeding, using reinforcing agent to the ovary of female parent is strengthened, to the parent is low temperature accumulated temperature, to the parent is handled, using hormone to control the gonad development state of parent, fertilization is carried out, obtains fertilized egg, fertilized egg hatches etc.Steps.By the treatment such as strengthening, low temperature accumulated temperature, cutting fin of parent fish, the gonad development state of parent fish is accurately controlled, the reverse season breeding of gold pompano is realized, the reproductive efficiency and fry quality of gold pompano are improved, solve the problem that existing gold pompano breeding is seasonally limited, with good economic benefits and application prospect.

The invention provides a rapid breeding method for trachinotus ovatus. The rapid breeding method comprises the following steps: (1) treatment of a breeding pond: aquatic plants are cultivated in the breeding pond; (2) fertilizer and water treatment: putting a fertilizer and water agent and composite algae species; (3) fry putting: putting the hatched fry into the breeding pond; (4) fry management, wherein fry management comprises a fry stage and a juvenile stage; in the fry stage, initial feed is put, and lamplight irradiation is adopted; in the juvenile fish stage, initial feed and juvenile fish mixed feed are put, and lamplight irradiation is adopted. The juvenile fish mixed feed comprises the following components in parts by weight: 25-40 parts of fish meal, 10-13 parts of corn pollen, 1-3 parts of sodium gluconate, 1-2 parts of galactooligosaccharide, 2-4 parts of theanine, 6-8 parts of mulberry leaf powder and 0.5-1 part of bacillus compound bacteria. By means of the rapid breeding method for the trachinotus ovatus, the breeding time of the trachinotus ovatus fries can be shortened, and the trachinotus ovatus fries with the body length of about 4 cm can be obtained within 20 days.

The invention discloses a specific InDel molecular marker primer for identifying the sex of a new variety of trachinotus ovatus and application, the primer is InDel-W or InDel-ZW, and the invention also discloses a method for identifying the sex of the new variety of trachinotus ovatus. According to the application of the primer or the method in sex identification of the trachinotus ovatus new variety and distinguishing of the trachinotus ovatus new variety and wild trachinotus ovatus, the primer is good in specificity and high in accuracy, does not harm trachinotus ovatus living bodies, can rapidly and accurately identify the sex of the trachinotus ovatus rapid growth new variety strain in batches at a time, and is high in practicability. The new strain group and other source groups can be distinguished, support is provided for identifying and distinguishing trachinotus ovatus group sources, and the method has important scientific research and application value.

This invention discloses a net cage for aquaculture of oval pomfret, comprising several floats, several filter plates, and a filter bottom plate. Each filter plate includes a frame and a filter plate, with the filter plate fixedly mounted on the frame. A sliding box is fixedly mounted on one side of the frame, and a turbine box is fixedly mounted on one end of the sliding box. The turbine box is fixedly connected to an inlet pipe and a drain pipe. The filter bottom plate is fixedly mounted on the bottom of the turbine box. Several filter plates and the filter bottom plate together form the net cage. A cleaning component for cleaning the filter plates is provided on the sliding box. During use, the operator turns on the water pump, and the water flow drives the turbine blades in the turbine box to rotate. The rotation of the turbine disk drives the rotating shaft to rotate, which in turn drives the bidirectional screw in the sliding box to rotate. The bidirectional screw drives a slider to reciprocate linearly on an anti-rotation rod via a swing block. During this reciprocating linear motion, the slider drives a brush plate fixedly mounted on the slider to clean the filter plates.

This invention relates to a fish interferon regulator IRF4 gene, protein, expression vector, and their applications, belonging to the field of fish molecular immunology technology. The fish interferon regulator IRF4 is derived from the oval pomfret, and the cDNA nucleotide sequence of the oval pomfret IRF4 gene is shown in SEQ ID NO.1. The amino acid sequence of the protein encoded by the oval pomfret IRF4 gene is shown in SEQ ID NO.2. This invention also provides an expression vector containing the oval pomfret IRF4 gene, and an anti-Vibrio harveyi infection preparation containing the protein or a eukaryotic expression vector. The oval pomfret IRF4 protein of this invention can promote the respiratory burst activity and phagocytic capacity of oval pomfret head kidney macrophages. Overexpression in oval pomfret... Believe After IRF4 is activated, the body's antibacterial ability can be significantly improved, and it can be used as an immune enhancer.

The invention discloses a targeted gill immunopotentiator for high-density cultured trachinotus ovatus as well as a preparation method and application of the targeted gill immunopotentiator, and belongs to the technical field of aquaculture. According to the invention, centella asiatica, dried orange peel, dandelion, common carpesium fruit, dried ginger, mangnolia officinalis, vitamin and guanidinoacetic acid are used as a formula to prepare the immunopotentiator capable of enhancing fish immunity. The immunopotentiator can protect the completeness of the gill, acts on the gill part, plays an immunoregulation role, and reduces the occurrence rate of gill diseases; the immunopotentiator contains bacteriostatic and anti-inflammatory components, and can inhibit reproduction of pathogenic bacteria and reduce inflammatory injury. Vitamin C, vitamin E and guanidinoacetic acid in the immunopotentiator can further enhance the ability of fishes to resist pathogenic microorganisms; the guanidinoacetic acid can also reduce the energy supply demand of protein decomposition, indirectly promote muscle protein synthesis, and improve the nutritional value of the trachinotus ovatus.