The invention discloses a pleurotus cornucopiae-planting cultivation medium containing camellia oleifera shells and a preparation method thereof. The preparation method comprises the following steps: selecting mildew-free camellia oleifera shells, enabling moisture content of the mildew-free camellia oleifera shells to be lower than 15% after sufficiently drying in the sun, crushing until the grain size of the camellia oleifera shells is 2 millimeters-5 millimeters, and taking the prepared camellia oleifera shells as one of components of the cultivation medium; the cultivation medium comprises the following components in parts by weight: 10-30 parts of camellia oleifera shells, 20-40 parts of bagasse, 20-40 parts of mulberry sawdust, 10 parts of biogas residue, 4 parts of corn flour, 2 parts of wheat bran, 2 parts of rice bran, 1 part of gypsum powder and 1 part of lime powder. The culture medium is bagged, sealed and sterilized to prepare the pleurotus cornucopiae-planting cultivation medium. The cultivation medium disclosed by the invention is low in cost of ingredients, easy in getting materials, simple in formula and preparation method, and can overcome a problem that production benefits of the pleurotus cornucopia are not high as cottonseed hulls rise in price; and moreover, the pleurotus cornucopiae-planting cultivation medium has the advantages of being low in pollution rate, quick to grow bacteria, good in quality of grown bacteria sticks, pure white and strong, white, strong and thick in mycelial growth, strong in growing power, uniform in growth speed, good in disease resistance, and the like.