The invention relates to a cavern environment-based
tricholoma matsutake artificial cultivation method, and relates to the technical field of
edible fungus cultivation, and the method comprises the following steps:
mushroom stick preparation: mixing
pine sawdust,
corncob,
bran, rice
bran and an additive, adding water, bagging, sterilizing with high-pressure steam or normal-pressure steam, and inoculating
tricholoma matsutake strains into each bag; environment construction: installing temperature and
humidity regulation and
light scattering equipment in
cave depots, natural caves and air-
raid shelters with the soil
layer thickness of more than 50 m, the constant temperature of 10-20 DEG C and the
humidity of 70-90%; in the
hypha stage, the temperature is controlled to be 15-18 DEG C, the
humidity is controlled to be 70-80%, and culture is conducted for 40-60 days; in the fruiting stage, the temperature is lowered to 12-16 DEG C in a stepped mode, the humidity is increased to 85-90%, and harvesting is conducted after fruiting bodies are induced to be formed. And recycling resources: crushing the waste
mushroom sticks, and mixing the crushed waste
mushroom sticks with soil according to a ratio of 1: 3 to prepare a
fertilizer or mixing 10-30% of a new material for reuse. The produced
tricholoma matsutake has the
tricholoma matsutake alcohol content of 77.21 mg / kg and the
polysaccharide content of 2.67%, the
active components of the
tricholoma matsutake are equal to those of wild
tricholoma matsutake, the
hypha germination rate is greater than or equal to 95%, the growth period is shortened to 40-60 days, and the
raw material consumption is reduced by