2852 results about "Edible mushroom" patented technology

The invention discloses an intelligent temperature and humidity regulation and control fungus cultivation method for edible fungus sticks, and relates to the technical field of agricultural Internet of Things. The problems that in the prior art, multi-variety / multi-stage adaptability is poor, sudden environment response lags behind, and equipment cooperation efficiency is low are solved. Cross-variety control strategy migration is realized through a multi-strain dynamic migration matrix and a migration learning algorithm, and a three-dimensional microenvironment map is generated in combination with a multi-source sensor network and Kriging interpolation; identifying a strain growth stage by using a time sequence convolutional network, loading a corresponding parameter set, and dynamically generating an anti-interference instruction and a power distribution sequence based on a double-delay depth deterministic strategy gradient algorithm and model predictive control; scheduling a conflict-free instruction queue by adopting a heterogeneous equipment collaborative framework, and updating model parameters through federated learning and a Shapley value evaluation closed loop; according to the invention, the intelligence, self-adaptability and precision of edible mushroom growth environment regulation and control are greatly improved, and the robustness of the system to environment mutation is enhanced.

The invention relates to the technical field of edible mushroom culture, in particular to an edible mushroom culture environment multi-dimensional monitoring system, which comprises an environment parameter acquisition module, a dynamic data preprocessing module, a multi-dimensional environment characteristic parameter extraction module, an environment abnormity evaluation module, a regulation and control decision generation module and an execution mechanism control module, the environmental parameter acquisition module acquires an original environmental parameter set; the dynamic data preprocessing module generates preprocessing environment data; the multi-dimensional environment characteristic parameter extraction module is used for extracting multi-dimensional environment characteristic parameters; the environment abnormity evaluation module outputs an evaluation result; the regulation and control decision generation module generates a regulation and control instruction sequence including priority ranking; and the execution mechanism control module converts the regulation and control instruction sequence into an execution mechanism driving signal. According to the method, the response efficiency of environmental regulation and the resource utilization rate are improved, and stable growth of edible mushrooms under variable environmental conditions is guaranteed.

The invention relates to the technical field of edible fungus cultivation and soilless culture, in particular to a preparation method and application of an organic ecological soilless culture substrate for dictyophora rubrovolvata. Auxiliary materials such as perlite, vermiculite, river sand and wood chips are mixed according to the proportion, and the soilless culture substrate is prepared. The matrix is completely used for soil-covered cultivation of dictyophora rubrovolvata, compared with a traditional cultivation mode, dictyophora rubrovolvata hyphae germinate quickly and grow vigorously, the conversion rate of mushroom buds (mushroom eggs) is high, the fruiting amount is large, meanwhile, the content of nutritional ingredients is increased, limited indexes such as heavy metal are obviously reduced, and the quality of dictyophora rubrovolvata products is effectively improved. According to the method, a new mode of soilless culture of edible mushrooms is constructed.

The invention relates to the technical field of biological control, in particular to bacillus velezensis JNJK-1, a microbial agent and application of the bacillus velezensis JNJK-1 and the microbial agent in edible fungus disease control, the bacillus velezensis JNJK-1 is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CMCC No.31075. The novel bacillus velezensis JNJK-1 is screened out for the first time, and the bacillus velezensis can be applied to biological control of edible mushroom diseases and has a broad-spectrum inhibition effect on pathogenic bacteria of edible mushrooms. Meanwhile, the inventor also finds that the bacillus velezensis JNJK-1, curcumin and sodium alginate are compounded into the microbial agent, so that the inhibition effect on the pathogenic bacteria of the edible fungi can be improved, and the microbial activity can be maintained.

The invention belongs to the technical field of edible mushroom cultivation, and particularly relates to a high-brittleness white flammulina velutipes HJ120 strain and application thereof. The technical problem to be solved by the invention is to cultivate a flammulina velutipes variety with better quality. According to the technical scheme of the invention, the high-brittleness white flammulina velutipes HJ120 is obtained, and the preservation number of the flammulina velutipes HJ120 is CCTCC (China Center for Type Culture Collection) NO: M 20242218. The invention also provides a food which comprises the white flammulina velutipes, spores of the white flammulina velutipes or / and an extract of the white flammulina velutipes.

The invention discloses an intelligent edible mushroom house environment control method, and belongs to the technical field of edible mushroom house environment control. According to the edible mushroom house environment intelligent control method, an acquisition module, a theoretical analysis module, a data comparison module, a generation module and an adjustment module are included, and the acquisition module, the theoretical analysis module, the data comparison module, the generation module and the adjustment module are connected. According to the intelligent control method for the environment of the edible mushroom house, the data comparison module compares the environment parameters monitored in real time with the optimal environment parameters according to the real-time environment change in the edible mushroom house, the change trend of the future environment is accurately predicted, and the environment parameters are optimized and adjusted, so that the intelligent control for the environment of the edible mushroom house is faster and more accurate; the defect of hysteresis of an existing system is overcome, the growth environment of the edible mushrooms is guaranteed, and then the yield and quality of the edible mushrooms are improved.

The invention discloses edible mushroom culture medium mixing equipment, and belongs to the technical field of edible mushroom culture medium processing, the edible mushroom culture medium mixing equipment comprises a shell, a discharge valve is fixedly connected to the lower surface of the shell, a motor is fixedly connected to the surface of the shell, a transverse plate is fixedly connected to the inner wall of the shell, and a connecting shaft is rotatably arranged in the transverse plate; a pipe sleeve is movably arranged on the lower surface of the connecting shaft, and mixing blades are fixedly connected to the surface of the pipe sleeve; a connecting rod is slidably arranged in the transverse plate, and the lower surface of the connecting rod is fixedly connected with a shifting plate moving in the horizontal direction. The upper portion of the shell is of an open structure, during working, raw materials are added into the shell, the motor is started at the same time, when the motor works, the belt pulley drives the connecting shaft, the pipe sleeve and the mixing blades to rotate, meanwhile, the pipe sleeve and the mixing blades move in the vertical direction, the mixing effect is optimized, and the finished product quality is better.

The invention discloses a method for preparing an organic fertilizer from livestock and poultry manure, which comprises the following steps: mixing cow dung and plant ash, pig manure and edible mushroom waste, chicken manure and vegetable waste, and duck manure and corn bran or mushroom stick residue, inoculating a corresponding mixed inoculant, fermenting and decomposing by using a trough type aerobic fermentation process, crushing, and screening to obtain the organic fertilizer. The cow dung bio-organic fertilizer, the pig manure bio-organic fertilizer, the chicken manure bio-organic fertilizer and the duck manure bio-organic fertilizer are obtained. According to the invention, rapid decomposition in 28 days in winter and 18 days in summer can be realized, meanwhile, the decomposition degree is remarkably improved, the killing rate of harmful germs reaches 98.93%, the humus content is greater than or equal to 58%, and the product is rich in microbial beneficial bacteria, rich in organic matters, mild in fertilizer efficiency and free of root burning.

The invention discloses an edible mushroom cultivation device, and relates to the technical field of agricultural planting, the edible mushroom cultivation device comprises two bases and a greenhouse frame, the greenhouse frame is fixedly connected to the upper surfaces of the two bases, a moving assembly is arranged on the bases, and the moving assembly comprises rails fixedly connected to the upper surfaces of the two bases; and four symmetrical moving plates are slidably connected to the two sides of the rail, four moving wheels are rotatably connected to the inner side of each moving plate, a moving block is fixedly connected between every two opposite moving plates through bolts, and a framework is fixedly connected to the tops of the moving blocks. Edible mushrooms are irrigated by moving and spraying on the framework, the situation that the load of a greenhouse frame is increased due to the fact that a spraying device needs to be arranged on the greenhouse frame in the prior art can be avoided, then the greenhouse frame is prevented from collapsing due to too large pressure, and the spraying device can be conveniently moved to the position where maintenance is convenient through the independent framework; and the maintenance difficulty and time cost are reduced.

The invention discloses a fungus extract capable of effectively regulating uric acid metabolism and relieving inflammation as well as a preparation method and application of the fungus extract, and belongs to the technical field of deep processing of edible fungi. Schizophyllumcommuneh and poplar phellinus sporocarp are mixed and extracted, and the prepared fungus extract can effectively inhibit activity of xanthine oxidase in serum and liver of mice, reduce content of uric acid, creatinine, urea nitrogen and the like in serum and promote excretion of uric acid, creatinine, urea nitrogen and the like in urine; meanwhile, the content of inflammatory markers such as IL-6 and TNF-alpha in mouse serum can be effectively reduced, inflammatory infiltration of liver and kidney cells is relieved, and the problem that an existing edible mushroom extract is not ideal in treatment effect on hyperuricemia is solved.

The invention belongs to the field of edible mushroom planting control, and particularly relates to a robot collaborative operation control method and system in the edible mushroom planting process, and the method comprises the steps: an inspection robot scans a mushroom bed, judges the maturity of edible mushrooms in combination with an image recognition algorithm, calculates the regional to-be-picked density, and generates a to-be-picked task list; according to the list, a preset parameter table and a curve, dynamically generating a coordinated picking task control instruction including the target coordinate, the number of the robots and the specific clamping posture, force and lifting speed of each robot; after the picking robot responds to the instruction to execute operation, a signal is fed back to the nearest inspection robot, secondary imaging confirmation is triggered, if residues exist, a supplementary picking list is generated according to the residue density, execution is repeated, and therefore efficient, accurate and lossless automatic cooperative harvesting of the edible mushrooms is achieved.

The invention provides a method for preparing an edible mushroom culture medium from agricultural waste biomass and application, and belongs to the technical field of agricultural resource utilization and edible mushroom culture. The edible fungus culture medium provided by the invention is prepared from the following components in parts by mass: 10 to 20 parts of wood dust, 15 to 30 parts of sawdust, 15 to 30 parts of corn straw, 20 to 30 parts of algae waste, 30 to 50 parts of cottonseed hull, 3 to 10 parts of chitosan, 3 to 10 parts of humic acid, 1 to 3 parts of gypsum, 1 to 3 parts of lime and 0.3 to 1 part of compound strain. By adopting the edible mushroom culture medium disclosed by the invention, the yield and the quality of mushrooms can be remarkably improved, and the quality of the mushrooms is effectively improved due to the addition of the algae waste, the humic acid and the chitosan. Meanwhile, the edible mushroom culture medium can also be used for cultivating other types of edible mushrooms such as oyster mushrooms and pleurotus eryngii, and the yield of the edible mushrooms is increased.

The utility model belongs to the technical field of yak lighting roofs, and relates to a multi-layer three-dimensional edible mushroom cultivation frame which comprises an installation base, the upper end face of the installation base is fixedly connected with four sets of supporting sliding rails, and the interiors of the four sets of supporting sliding rails are connected with connecting blocks in a sliding mode. A threaded rod is rotated through a hand wheel, a sliding block slides in a sliding block support through the threaded rod, and then a supporting frame is rotationally connected with the sliding block through a second rotating shaft, a supporting rod and a first rotating shaft and used for pushing the supporting frame, so that the supporting frame slides on a supporting sliding rail through a connecting block; the supporting frames are symmetrically arranged in two groups relative to the sliding rail support, are used for adjusting and controlling the distance between the supporting frames, and are suitable for cultivation work of different types of edible mushrooms in different growth stages, so that the cultivation frame can continuously provide a suitable growth environment for the edible mushrooms, and cultivation management can be more conveniently carried out by adjusting the distance between the supporting frames.

The invention belongs to the technical field of edible mushroom cultivation, and particularly relates to a high-brittleness yellow flammulina velutipes HJ277 strain and application thereof. The technical problem to be solved by the invention is to cultivate a flammulina velutipes variety with better quality. According to the technical scheme, the yellow flammulina velutipes HJ277 with the high brittleness is obtained, and the preservation number of the yellow flammulina velutipes HJ277 is CCTCC (China Center for Type Culture Collection) No: M 20242219. The invention also provides a food which comprises the yellow flammulina velutipes, spores of the yellow flammulina velutipes or / and an extract of the yellow flammulina velutipes.

The invention belongs to the field of agricultural engineering, and particularly relates to an intelligent edible mushroom incubator based on an artificial intelligence internet of things. Comprising an edible fungus culture box body, and an edible fungus growth frame module, a spray atomization module, a carbon dioxide module, a fresh air circulation module, a visual detection module, a growth light module, a temperature sensing module and an intelligent disinfection module which are arranged on the edible fungus culture box body in an assembling manner, the upper surface, the lower surface, the left surface, the right surface and the rear surface of the edible mushroom incubator body are provided with heat preservation interlayers, and the front side surface is of a double-open cold storage door structure. By means of the Internet of Things technology, the artificial intelligence technology and the like, real-time monitoring of the growth environment of the edible mushrooms and accurate mastering of the growth condition of the edible mushrooms are achieved, so that the production efficiency and yield of the edible mushrooms are improved, and meanwhile the production cost and risk are reduced.

The invention discloses a mushroom yield estimation method based on three-dimensional reconstruction lossless phenotype parameter extraction, belongs to the technical field of agricultural informatization and intelligentization, and solves the problem of low mushroom yield estimation accuracy. The method comprises the following steps: performing point cloud three-dimensional reconstruction on shiitake mushrooms by using a region of interest to generate dense point cloud data; preprocessing the dense point cloud data; based on an improved CP-PointNet + + model and a clustering algorithm, segmenting mushroom point cloud, extracting multi-dimensional key phenotypic parameters of pileus diameter, thickness and stipe height, and covering main features of mushroom phenotypic information; the improved PointNet + + network comprises the following steps: adding a CBAM module to an MLP layer of the PointNet + + network, and replacing common convolution with partial convolution PConv; and taking the phenotypic parameters of the shiitake mushrooms as input variables of the neural network model, and predicting the shiitake mushroom yield. The method is suitable for phenotypic character analysis and breeding research of edible mushrooms such as shiitake mushrooms and the like.

The invention provides actinomycetes and application thereof in prevention and control of fungal diseases of edible fungi. The actinomycetes are streptomyces cellulosae, the strain number of the actinomycetes is P8-2, and the registration number of the actinomycetes in the China General Microbiological Culture Collection Center is CGMCC No.32350, hereinafter referred to as streptomyces cellulosae CGMCC No.32350. Experimental results show that the streptomyces cellulosae CGMCC No.32350 disclosed by the invention has an inhibition effect on the fungal diseases of the edible mushrooms, including Trichoderma koningii, T.harzianum and Fusarium equiseti, and the streptomyces cellulosae CGMCC No.32350 has no influence on the growth of the edible mushrooms.

The utility model discloses an edible fungus strain constant temperature incubator, which relates to the technical field of incubators, and comprises an incubator body, a limiting block, a thermal insulation layer, an auxiliary frame, a heating device, a fixed plate, a partition plate and a rotating block, the rotating block is positioned in a sliding block, side plates are arranged on two sides of the sliding block, and a top cover is connected above the incubator body. A ventilation opening is further formed in the upper portion of the top cover, a movable block is further arranged in the limiting block, an ultraviolet lamp is connected to the front side of the movable block, adsorption magnets are connected to the two sides of the movable block, and an elastic belt is arranged above the movable block. The multiple sliding blocks and the rotating blocks are arranged in the cultivation box body, the multiple fixing plates can be taken out by taking out the sliding blocks when the fixing plates need to be taken out, strains above the fixing plates are prevented from being damaged, meanwhile, the multiple rotating blocks can change the height of the fixing plates to adapt to different types of strains, and the practicability is high. And the culture work of the strain is ensured to be carried out.

The invention belongs to the technical field of edible fungus cultivation, and discloses an automatic bag removing device for edible fungus matrixes, which comprises a processing table, a six-edge support is fixedly mounted on the processing table, lifting cylinders are fixed on the upper and lower end faces of the six-edge support, and cutting knife rests are fixed at the output ends of the lifting cylinders. Six groups of tearing structures for clamping and expanding the bag body are mounted on the six-edge bracket at equal angles; electric sliding rails are symmetrically fixed to the two sides of the upper surface of the machining table, a sliding support is slidably installed on the electric sliding rails, an outer positioning ring is fixed to the sliding support, a rotating frame is rotatably installed in the outer positioning ring, and a limiting structure for clamping and limiting a bag body filled with edible fungus matrixes is arranged on the rotating frame. The bag body can be clamped, expanded and cut apart, so that the bag body is effectively torn and separated from a mushroom stick, damage to hyphae in the mushroom stick is avoided in the separation process, and the bag removing effect is good.

The utility model discloses an edible mushroom compost stirring device, and particularly relates to the technical field of edible mushroom compost, the edible mushroom compost stirring device comprises a containing hopper, a stirring barrel and a mounting plate, the stirring barrel is arranged at the top of the containing hopper, the mounting plate is mounted at the top end of the stirring barrel, and a center rod is arranged in the stirring barrel in a penetrating manner; an adjusting cylinder is sleeved with the top end of a stirring cylinder used for containing, the interior of the adjusting cylinder is closed, connecting blocks are fixedly connected to the outer walls of the two sides of the top ends of the stirring cylinder and the adjusting cylinder, and the bottom end of the stirring cylinder is slidably sleeved with a bottom plate; fixing plates are fixedly connected to the two ends of a mounting plate used for supporting, a plurality of electric push rods are arranged in the two ends of the mounting plate in a penetrating mode, and the bottom extending ends of the electric push rods are fixedly connected with connecting blocks. The stirring device has the advantages that the stirring operation does not need to be paused during discharging, the accommodating size is convenient to adjust, and the stirring device is suitable for stirring different materials.

The utility model relates to the technical field of edible fungi, and discloses a fungus cultivation box capable of controlling temperature, which comprises a thermotank body, a control end and a box door. Through cooperative use of the adjusting assembly and the fixing mechanism, a user opens a box door, pulls out a fixing rod, leaves the fixing rod from an inner cavity of a clamping groove, places a container on the top of a placing plate, and then starts an electric push rod, the electric push rod drives a movable rod to move, and the movable rod extrudes a movable block; after being stressed, the movable blocks drive the clamping plates to move and extrude the springs, the clamping plates make contact with the containers, then the fixing rods are reset, the fixing rods enter inner cavities of the clamping grooves, limiting is completed, and the problems that according to most existing edible mushroom culture constant-temperature boxes, the containers containing edible mushrooms are directly placed on the placing plates, the containers are not limited, and the edible mushroom culture constant-temperature boxes are not limited are solved. And when the constant-temperature box is subjected to external force, the constant-temperature box deviates and even topples over, so that the cultivation process is influenced.

The invention relates to the technical field of edible mushrooms, and discloses an auricularia polytricha strain and application thereof. The Auricularia polytricha Ji 5 disclosed by the invention is preserved in the China Center for Type Culture Collection on October 23, 2024, and the preservation number of the Auricularia polytricha Ji 5 is CCTCC (China Center for Type Culture Collection) NO: M 20242312. The auricularia polytricha Ji 5 sporocarp has excellent agronomic characters such as less fluff, regular auricular piece edge, small auricular base and high rehydration rate, can be used for screening breeding, cultivation planting and fermentation extraction of auricularia polytricha, and can also be used for deep processing of food or health care products.

According to the edible mushroom bud detection and collection system, method, medium and device, the advanced sensing technology and the image recognition technology are adopted, the growth state and the production orientation of mushroom buds can be accurately detected, the collection action is accurately controlled, the collection process is optimized, damage to edible mushrooms is reduced, and the collection efficiency and quality are improved. Through an accurate detection technology and an efficient collection means, accurate identification and automatic collection of mushroom buds are realized. Therefore, intelligent and standardized operation of mushroom bud detection and collection in edible mushroom production is realized, and powerful guarantee is provided for high-quality production of edible mushrooms.

The invention relates to the technical field of edible mushroom cultivation, in particular to a culture medium for cultivating stropharia rugoso-annulata and a preparation method and application thereof. The culture medium for cultivating the stropharia rugoso-annulata is prepared from the following components: rape straw, soybean meal, rice chaff, pepper straw, rice straw, loofah sponge, livestock and poultry manure, corncob, sawdust, calcium lignosulphonate and gypsum powder. The time from sowing to fruiting is 40-55 days by adopting the stropharia rugoso-annulata culture medium provided by the invention, and the fruiting time is obviously shortened compared with that of a contrast. By adopting the culture medium for cultivating the stropharia rugoso-annulata, the average yield of the stropharia rugoso-annulata reaches 8.15-8.64 kg / m < 2 >, and compared with a contrast, the average yield of the stropharia rugoso-annulata is remarkably improved. Therefore, the oilseed rape straw, the soybean meal, the rice chaff, the pepper straw, the rice straw, the loofah sponge and the calcium lignosulphonate are compounded, the fruiting time of the stropharia rugoso-annulata is shortened, and the yield of the stropharia rugoso-annulata is increased.

The invention relates to the technical field of cultivation equipment, and discloses an automatic edible mushroom cultivation device which comprises a cultivation box which serves as a basic assembly and bearing component of the automatic edible mushroom cultivation device and is used for assembling and bearing all treatment mechanisms. The intelligent sprinkling irrigation mechanism is arranged at the upper part of the cultivation box and is used for performing intelligent sprinkling irrigation treatment on the edible mushrooms cultivated in the device; the growth assisting mechanism is arranged in the cultivation box and used for conducting auxiliary treatment on the edible mushrooms cultivated in the device in the growth process. By adding and arranging the intelligent sprinkling irrigation mechanism, the intelligent sprinkling irrigation mechanism can accurately collect temperature and humidity data in equipment in real time by means of a sensing technology during cultivation of edible mushrooms, deeply analyzes the collected data through a built-in intelligent analysis system, accurately judges the current growth requirements of the edible mushrooms, and then determines the growth requirements of the edible mushrooms on the basis of analysis results. The sprinkling irrigation operation can be carried out in a targeted manner.

The invention discloses a yellow flammulina velutipes ZJJZG002 and InDel marker with high resistance and high brittleness and application of the yellow flammulina velutipes ZJJZG002 and InDel marker, and relates to the technical field of edible mushroom cultivation, the preservation number of the yellow flammulina velutipes is CGMCC NO.41174, and the yellow flammulina velutipes is classified and named as flammulina velutipes. The strain ZJJZG002 takes yellow flammulina velutipes F24 and white flammulina velutipes W1 as parents, the parent materials are subjected to ultraviolet mutagenesis treatment and then are subjected to single spore isolation, hybridization (a hybrid combination XB51 * YU19) and fruiting tests, and the strain ZJJZG002 is not prone to umbrella opening, rich in mushroom fragrance, crisp and refreshing in taste, neat in fruiting, suitable for production in various cultivation modes and capable of injecting a new seed source for the domestic flammulina velutipes market.

The invention discloses a method for cultivating coprinus comatus by using white spirit vinasse, and belongs to the technical field of edible mushroom production. In order to realize production of coprinus comatus by using vinasse instead of cottonseed hulls, the invention provides a method for cultivating coprinus comatus by using liquor vinasse, which comprises the following steps: uniformly mixing oyster mushroom bran, vinasse with the pH value of 9-10 and bran, and adjusting the moisture to 55-65% to obtain a cultivation material; after sterilization, coprinus comatus strains are inoculated, and the coprinus comatus strains are cultured under the conditions that the temperature is 25-31 DEG C, the relative air humidity is 65-75% and no light exists until hyphae grow fully; then adding a soil covering material, and culturing for 8-10 days; and irradiating with scattered light at the temperature of 16-22 DEG C and the relative air humidity of 85-90%, culturing for 7-13 days, and picking. By optimizing the cultivation material formula and the mycelium culture temperature, the growth cycle of the coprinus comatus is shortened, the fruiting rate of the coprinus comatus is increased, the mushroom quality is improved, the production cost is reduced, and a new way is provided for edible mushroom production.

The invention discloses a control method of a full-automatic single-bag tying all-in-one machine for edible mushrooms. The method comprises the following steps: step 1, making a bag; 2, bag moving and bag opening are carried out; step 3, bagging; after the clamping jaw assembly opens the fungus bag, the clamping jaw mechanism and the fungus bag are driven by a servo motor to move to the opening of the discharging barrel along the Y axis, at the moment, gaps between the two sides of the clamping jaw and the discharging barrel must be uniform and do not interfere with each other, then the clamping jaw drives the fungus bag to move to a bag removing position from the opening of the discharging barrel along the X axis, and at the moment, the bagging action is completed; 4, resetting the clamping jaw; after bagging is completed, the clamping jaw air cylinder retracts, meanwhile, the clamping jaw mechanism moves to the bag opening axis along the Y axis, and a rolling wheel on the movable clamping jaw moves to the outside of the discharging barrel along an annular ring on the discharging barrel assembly; step 5, bagging; and 6, tying the opening. The production line has the advantages of being high in automation degree, high in equipment applicability, good in produced mushroom stick quality, high in production efficiency, good in equipment stability, convenient to maintain and easy and convenient to operate.

The invention belongs to the technical field of genetic engineering of edible mushrooms, and particularly relates to a construction method of an efficient and stable genetic transformation system of flammulina velutipes. The construction method comprises the following steps: culturing in an MM culture medium by taking a flammulina velutipes strain 0747 as a receptor; the pFgnpt vector is converted into agrobacterium AGL1 (AGL1); inoculating hyphae into an IM liquid culture medium containing acetosyringone and MES, mixing with agrobacterium tumefaciens, culturing, and transferring the hyphae to an IM solid flat plate paved with filter paper; hyphae are transferred to a PDA resistance plate containing G418 and cephalosporin for resistance screening, and an efficient and stable flammulina velutipes genetic transformation system is obtained. The new selection marker G418 is developed and applied, limitation of a traditional selection marker is broken, more choices are provided for flammulina velutipes genetic transformation, a new thought is provided for genetic transformation research of other edible mushrooms, and the application field of an agrobacterium tumefaciens-mediated transformation technology is expanded.

The invention discloses an elastic claw reel type edible mushroom stick picking device, and particularly relates to the technical field of mushroom stick rotary picking, the elastic claw reel type edible mushroom stick picking device comprises a picking box, a rotating shaft, two bearing sleeves and an inclination adjusting rotary picking mechanism; the inclined adjusting rotary picking mechanism comprises a plurality of reels, a plurality of picking elastic racks, a linkage block, a sleeving sliding block, a sliding frame and a picking limiting plate, and further comprises a shoveling picking adjusting mechanism and a limiting picking adjusting assembly. According to the invention, through the inclination adjustment rotary picking mechanism, a plurality of picking elastic racks can be obliquely embedded into a picking position with a specified depth, mushroom sticks can be thoroughly and rotatably picked and taken out from a soil depth position in the picking process, the adjustable accuracy of mushroom stick picking is greatly improved, the mushroom sticks are picked more thoroughly, and the picking effect is better; therefore, the problems that the mushroom stick picking adjustable accuracy is poor, picking is not thorough, and the picking effect is poor are solved.