The application discloses a method for evaluating cross-generation effect of dietary intervention through vertical transmission of
flora, and belongs to the technical field of the cross of
nutrition and microbiomics. The method comprises the following steps: subjecting female experimental animals to at least three different
dietary interventions; collecting
feces as a maternal sample before intervention and before
mating; after
pregnancy, continuing to give the corresponding dietary intervention until natural delivery, and collecting the
offspring feces as an
offspring sample on the day when the
offspring is weaned; performing high-
throughput sequencing on the collected samples, analyzing the
flora structure by
bioinformatics, identifying the genera that are common to the maternal and offspring, have significant differences in abundance between different dietary groups, and have biological correlation between the genera function and the evaluated health
phenotype as the diet-dependent vertical transmission core genera; and then performing
correlation analysis with the offspring health
phenotype data, if there is a correlation, it is judged that the material to be evaluated affects the offspring health through the vertical transmission of
flora. The application provides a general methodological tool for studying the diet-flora-cross-generation
health effect.