This invention belongs to the field of microalgal
cell motility analysis and detection, specifically relating to a method and application for assessing the
spore motility activity of the marine parasitic
dinoflagellate *
Heliotropium indicum* using
trajectory analysis. The method includes steps such as
microscopic image acquisition, video tag re-identification for
image sequence grouping, image preprocessing (
grayscale conversion, filtering and
noise reduction,
thresholding, hole filling, and overlap segmentation), trajectory detection based on the nearest
neighbor algorithm, and
visualization of trajectory and velocity data output. The
spore cell motility activity detection method proposed in this invention can be used to evaluate the spread and infective capacity of *
Heliotropium indicum*, as well as assess the killing effect of different
drug types and dosages, providing
technical support for taking corresponding control measures. This detection technology is practical, efficient, accurate, and reliable, and is expected to be widely applied in practice, promoting the healthy and
sustainable development of
aquaculture.