440 results about "Acetylcholinesterase" patented technology

The invention relates to an antisense oligonucleotide targeted to the coding region of the human acetylcholinesterase (AChE), which selectively suppresses the AChE-R isoform of the enzyme. The antisense oligonucleotide is intended for use in the treatment and / or prevention of neuromuscular disorders, preferably myasthenia gravis. In addition, it can penetrate the blood-brain barrier (BBB) and destroy AChE-R within central nervous system neurons, while also serving as a carrier to transport molecules across the BBB.

RNA interference is provided for inhibition of ocular hypertension target mRNA expression for lowering elevated intraocular pressure in patients with open-angle glaucoma or ocular hypertension. Ocular hypertension targets include carbonic anhydrase II, IV, and XII; β1- and β2 adrenergic receptors; acetylcholinesterase; Na+ / K+-ATPase; and Na—K-2Cl cotransporter. Ocular hypertension is treated by administering interfering RNAs of the present invention.

RNA interference is provided for inhibition of ocular hypertension target mRNA expression for lowering elevated intraocular pressure in patients with open-angle glaucoma or ocular hypertension. Ocular hypertension targets include carbonic anhydrase II, IV, and XII; β1- and β2 adrenergic receptors; acetylcholinesterase; Na+ / K+-ATPase; and Na—K-2Cl cotransporter. Ocular hypertension is treated by administering interfering RNAs of the present invention.

The present invention provides a novel compound having an excellent acetylcholinesterase inhibitory effect. That is, it provides a 4-substituted piperidine compound fluoride represented by the following formula, a pharmaceutically acceptable salt thereof or hydrates thereof (provided that 1-benzyl-4-[(5,6-dimethoxy-2-fluoro-1-indanon)-2-yl]methylpiperidine, a pharmaceutically acceptable salt thereof and hydrates thereof are excluded).wherein R<1 >and R<2 >represent substituents.

A sensor and method for detecting biological and chemical agents comprising metal interdigitized electrodes coated with hybrid polymer-based conducting film and an instrument for applying electrical voltage to the electrodes and registering the change in electrical current. The hybrid film also comprises indicator biomolecules encapsulated within the film or attached to it. The bioindicator molecules preferably comprise enzyme acetylcholinesterase. When these indicator biomolecules come in a contact with a pathogen, chemical and / or morphological changes occur in the film and electrical current flowing through the electrodes is modulated. The pathogen comprise inhibitors of enzymes, preferably organophosphates, thiophosphates or phosphonates. The change in current indicates the presence of a biological and chemical agent and is registered.

Agents for improving potentcy of the urinary bladder which comprises an amine compound of non-carbamate-type having an acetylcholinesterase-inhibiting action. Particularly, crystals of a tricyclic, condensed, heterocyclic derivative are provided, which possess an excellent action to inhibit acetylcholine esterase and an action to improve the excretory potency of urinary bladder. As an example, crystals of of 8-[3-[1-[(3-fluorophenyl)-methyl]-4-piperidinyl]-1-oxopropyl]-1,2,5,6-tetrahydro-4H-pyrrolo[3,2,1-ij]quinolin-4-one or a salt thereof and pharmaceutical compositions containing them are disclosed.

The present invention relates to methods and compositions for reactivating Acetylcholinesterase in living organisms using contents from the Morinda citrifolia L. plant. More particularly, the present invention relates to methods and compositions involving the reactivation of Acetylcholinesterase using fruit juice obtained from the Morinda citrifolia L. plant.

RNA interference is provided for inhibition of ocular hypertension target mRNA expression for lowering elevated intraocular pressure in patients with open-angle glaucoma or ocular hypertension. Ocular hypertension targets include carbonic anhydrase II, IV, and XII; β1- and β2 adrenergic receptors; acetylcholinesterase; Na+ / K+-ATPase; and Na—K-2Cl cotransporter. Ocular hypertension is treated by administering interfering RNAs of the present invention.

The invention relates to a novel scutellarin aglycon 4 (1)-position carbamate derivant (1), a preparation method and the application thereof. A pharmacological experiment proves that the compounds have obvious inhibitory activity of acetylcholinesterase and have protective effect with different degrees on PC12 cell trauma induced by H2O2, so the compounds can be used for preparing the drugs for treating neurodegenerative diseases such as vascular dementia, AD (presenile dementia), etc.

The invention provides construction of a surface plasma resonance sensor based on the amplification effect of a magnetic molecularly imprinted polymer and the application of the surface plasma resonance sensor in pesticide detection, and belongs to the technical field of surface plasma resonance sensing. Chlorpyrifos is regarded as a template molecule firstly; the magnetic molecularly imprinted polymer which has recognition action on the chlorpyrifos is compounded by adopting the self-polymerization performance of dopamine; after elution is performed on the chlorpyrifos, the magnetic molecularly imprinted polymer containing imprinted cavities has good superparamagnetism and high selectivity; the specific interaction between acetylcholinesterase fixed on the surface of a gold sheet and the chlorpyrifos is adopted to construct a pesticide residue recognition, detection and sensing interface on the basis of the amplification effect of the magnetic molecularly imprinted polymer. Compared with the conventional pesticide residue detection technology, the surface plasma resonance sensor on the basis of the amplification effect of the magnetic molecularly imprinted polymer has high detection sensitivity and good selectivity to chlorpyrifos detection, and has an excellent application prospect.

The invention relates to a preparation method and application of 9-bit substituent double-functional group berberine derivatives. In-vitro biological activity assay research shows that the 9-bit substituent double-functional group berberine derivatives provided by the invention has acetylcholinesterase inhibition activity higher than that of berberine serving as a leading compound, and also has higher inhibition activity on butyrylcholine esterase. After being further optimized and screened, the derivatives have favorable foreground of being developed into medicaments for treating diseases such as AD disease, vascular dementia, cognitive dysfunction and the like. The general formula of the derivatives is shown as the following formula, wherein X, Y and Z are defined in specifications.

A toxic material detection apparatus includes a sample collection portion including a sample inlet and a sample concentrator adapted to concentrate an environmental sample on a substrate. A sample distributing system transfers portions of the substrate to a color sensor and an ion mobility spectrometer for simultaneously analysis and toxin detection, particularly cholinesterase inhibitor detection. Optionally, a portion of the substrate may be directed to an archive for possible analysis at a later time. Reagents utilized include the enzyme acetylcholinesterase (AChE), and the reactants acetylthiocholine iodide (ATCI) and 5,5′-dithio-bis-(2-nitrobenzoic acid) (DTB). A data management unit provides for near-real-time analysis of the samples in under 5 minutes. Simultaneous “hits” by both analysis methods indicate the presence of a cholinesterase inhibitor.

The reagent kit for detecting pesticide residue includes acetylcholinesterase, protecting agent, primer and developing agent. The single or purified monopolymer acetylcholinesterase highly sensitive to pesticide is obtained from sensitive housefly line head. The reagent kit may be maintained at normal temperature and has high sensitivity and high measurement accuracy in detecting pesticide residue in vegetable and fruits.

The invention relates to an acetylcholinesterase biosensor and an application thereof, and belongs to the technical field of electrochemical detection. The biosensor is produced through the following steps: 1, preparing a MnO2-nanoflakes composite nano-material and a Pd-Pt@MnO2-nanoflakes composite nano-material from KMnO4, CTAB, PdCl2, PEG400, H2PtCl6, sodium citrate and sodium borohydride used as initial raw materials through a water bath technology; and 2, immobilizing acetylcholinesterase by using chitosan (CS), and immobilizing acetylcholinesterase (AChE) on the surface of a Pd-Pt@MnO2 modified glassy carbon electrode through adopting physical adsorption to construct the electrochemical biosensor. The biosensor adopting an electrochemistry and enzyme sensing combination technology realizes high-sensitivity detection of methyl parathion and carbofuran pesticides, has the advantages of small amount of a required sample, short detection time, high sensitivity and low cost, and is suitable for analyzing and detecting pesticide residual.

The invention relates to the technical field of sensors, in particular to an acetylcholinesterase biological sensor. A method for manufacturing an electrochemical acetylcholinesterase biological sensor comprises the following steps of: manufacturing an enzyme membrane; modifying a glassy carbon electrode by using functionalized multi-wall carbon, namely functionalizing a multi-wall carbon nanotube, cleaning, testing and modifying; and fixing the enzyme membrane. Due to the technical scheme, the acetylcholinesterase biological sensor manufactured by the method has the advantages of simple structure, high sensitivity, repeated detection and the like.

The present invention provides a quick reflection test card and making method of pesticide residue, which can adapt the acetylcholinesterase with extracting the serum of human as enzyme piece and measure the sensibility of organophosphorus and carbamate pesticide ranged from 0.001 to 1.2mg / kg, wherein the quick reflection test card is a rather sensitive enzyme source. This invention discloses a good heat-stability enzyme source, which lasts 7-8 hours by adding gelatin stabler or trehalose in more or less 90 deg.c circumstances.

The invention discloses a magnetic electrode based on Fe3O4-AuNPs magnetic composite nanoparticle immobilized acetylcholinesterase, belonging to the technical field of biosensors. Fe3O4-AuNPs magnetic composite nanoparticles are modified on a magnetic glassy carbon electrode through a magnetic force, and the magnetic glassy carbon electrode is used as a carrier to immobilize the acetylcholinesterase. A preparation method of the magnetic electrode comprises the following steps: firstly, preparing Fe3O4-AuNPs, then, preparing the magnetic glassy carbon electrode, dripping and coating 0.10 g / L CNTs (Carbon Nanotubes) turbid liquid, airing, then, putting the MGCE (Magnetic Glassy Carbon Electrode) in Fe3O4-AuNPs turbid liquid, adsorbing, washing, airing, and dripping and coating glutaraldehyde and AChE. The electrode disclosed by the invention has good conductivity and electrochemical properties.

The invention relates to a kit for quickly detecting toxicity of pesticide residue, in particular to a kit for quickly detecting the toxicity of pesticide residue containing a mixture of acetylcholinesterase and butyryl cholinesterase. The kit consists of choline esterase, a substrate, a color-developing agent 5,5'-dithio-2-2'-dinitrobenzoic acid (DTNB) and phosphate buffer solution, wherein the choline esterase is the mixture containing the acetylcholinesterase and the butyryl cholinesterase, and the substrate contains butyrylthiocholine iodide.

A circulatory long-lived cholinesterase (ChE) protein, such as acetylcholinesterase (AChE) or butyrylcholinesterase (BChE), which is a ChE protein modified with a non-antigenic polymer. The ChE may be AChE, such as native AChE of mammalian origin or of non-mammalian origin, or recombinant AChE. The recombinant AChE may be mutated at one or more amino-acid residues. The BChE may be native BChE of mammalian origin or of non-mammalian origin.

The invention relates to a method and a device for detecting pesticide by using cholinesterase. More specifically, the invention relates to a novel pesticide detection method based on acetylcholinesterase-fixed magnetic nanoparticles, wherein the magnetic nanoparticles serve as carriers to collect the acetylcholinesterase in solution. The invention also relates to a portable pen-shaped detector adopting the method for detecting pesticide.

A process for preparing the acetylcholinesterase used to detect the content of residual agricultural chemical in fruit and vegetable includes such steps as extracting the coarse liquid extract from animal blood, purifying by ammonium sulfate deposition method, removing ammonium sulfate by dialysis method, concentrating and drying. Its advantages are widely available raw material, low cost and high purity and sensitivity of product.

The present invention is directed to a quantum dot nanoparticle-based universal neurotoxin biosensor having three components (a) a dendrimer-encapsulated quantum dot nanoparticle; (b) acetylcholinesterase; and (c) an acceptor fluorophore, wherein the quantum dot nanoparticle and the acceptor fluorophore linked to the acetylcholinesterase.

The invention relates to marine fungi aspergillus terreus C23-3, a fermentation liquor active extract as well as a preparation method and application thereof. The marine fungi aspergillus terreus C23-3 is collected in the Guangdong Microbiological Culture Collection Center (GDMCC) on Jan 22th, 2018 with the collection number GDMCC No. 60316. Active substances of standing fermentation liquor of theaspergillus terreus C23-3 are subjected to extraction and purification; a fermentation liquor total extract of a bacterial strain can well resist oxidization and restrain the activity of acetylcholinesterase; an active extract prepared by purifying the fermentation liquor of the bacterial strain has very high oxidization resistance and / or acetylcholin esterase restraining activity, and has a very good application prospect on the aspect of preparation of an anti-senile dementia medicament and on the aspect of preparation of an anti-oxidization and / or acetylcholin esterase activity restrainingmedicament.

The invention provides reagents for detecting AChE (acetylcholinesterase), comprising deionized water, rhodamine B-modified gold nanoparticles, thioacetylcholine, and material for preventing aggregation of gold nanoparticles. The invention further provides a preparation method of the reagents for detecting AChE.

The invention belongs to the technical field of food safety, and particularly relates to a quick detection card for pesticide residues based on naked eye visual colorimetric determination. The quick detection card comprises fixed carrier membranes, a sample adding piece, a substrate piece and a detection strip, wherein acetylcholinesterase is immobilized on the sample adding piece; acetylthiocholine iodide is immobilized on the substrate piece; equal-interval colored tapes are arranged on the detection strip. The colored tapes on the detection strip can be faded through a product obtained after the acetylthiocholine iodide is hydrolyzed by the acetylcholinesterase, and certain pesticides have an inhibiting effect on the activity of the acetylcholinesterase. The quick detection card is based on the above principle, and pesticide residues can be semiquantitatively detected through counting the number of faded colored tapes on the detection strip; the quick detection card can be used for detecting common pesticide residues, is high in sensitivity, low in cost, and simple and quick to operate, improves the qualitative detection of the conventional quick detection card into semiquantitative detection, can be used for determining pesticide residues in samples such as vegetables, fruits and soil.

The present invention relates to novel alternative forms of human acetylcholinesterase (AChE) and nucleotide sequences encoding the same. The genes encoding the novel forms of human AChE have been identified in various malignant tumor cells. In a further aspect, the invention relates to a transgenic animal assay system for evaluating efficacy of drugs against cholinergic proteins, prior to or in the course of therapeutic treatment. Transgenic animals, preferably developing tadpole of Xenopus or mice which express human AChE, are used. The transgenic animal assay system is also useful for evaluating the toxicity of substances which potentially block human AChE (e.g. organophosphorous compounds).

The invention discloses a preparation method of a micro-enzyme reactor based on magnetic functionalized graphene oxide, and application of the micro-enzyme reactor in pesticide detection, belonging to the technical field of micro-fluidic control chips. The micro-enzyme reactor which can be applied to pesticide detection is prepared by firstly, loading Fe3O4 magnetic nano particles on the surface of graphene oxide by using a one-step in-situ synthesis method, so as to prepare a graphene oxide / Fe3O4 nano composite material with both good magnetism and biocompatibility, secondly, fixing acetyl choline esterase on the surface of the graphene oxide / Fe3O4 through action of pi-pi, hydrogen bonds and water repelling and the like, and fixing a graphene oxide / Fe3O4 / acetyl choline esterase compound inside a PDMS (Polydimethylsiloxane) micro chip channel through the action of an external magnetic field. Based on the inhibition principle of pesticide to acetyl choline esterase, the rapid quantitative detection on dimethoate is realized. The micro-enzyme reactor which is prepared based on a graphene oxide / Fe3O4 / acetyl choline esterase composite material is rapid in action, simple to operate and good in reproducibility, and an effective means is provided for rapid and sensitive detection on pesticide.

The invention relates to a rapid detection method for residual pesticides. The invention reveals an acetylcholinesterase-based color-developing method for the first time, and in the method, a special color-developing agent is adopted, so that negative and positive color-developing results are extremely obvious, and can be directly judged by naked eyes without using a spectrophotometer, therefore, the method has a good application prospect in the field of rapid detection on residual pesticides.