50 results about "Acetylcholinesterase" patented technology

The application relates to a preparation method of a biosensor. The application relates to a preparation method of a portable pesticide detection biosensor based on acetylcholinesterase (AChE)@poly(allylamine hydrochloride)-hydrogen bond organic framework (PAH-HOF) and application of the biosensor in on-site rapid chlorpyrifos detection, and belongs to the technical field of biosensors. According to the method, AChE is combined with poly(allylamine hydrochloride) (PAH) to change the surface charge and promote self-assembly of the AChE and the hydrogen bond organic framework (HOF), so that an AChE@PAH-HOF composite material with high catalytic activity, high embedding rate and high stability is formed; then the material is integrated into a water-retaining glycerol-sodium alginate hydrogel (G-SA) matrix to construct a disc-shaped sensor. The sensor can be attached to the surface of plants and used for long-term in-situ monitoring of organophosphorus pesticides. In combination with an image processing algorithm, the system realizes accurate tracking of the chlorpyrifos degradation process on the surface of tomato plants, and the detection limit reaches 1 ng / mL. The application provides non-invasive and low-cost technical support for crop health management and sustainable precision agriculture.

The present application belongs to the technical field of pharmaceutical therapy, and particularly relates to an amide-substituted tryptophan ketone derivative, a preparation method and application thereof. The structural formula of the tryptophan ketone derivative is shown in formula (A) or formula (B). In the formula, n=1 or 2, and R is any one of dimethylamine group, diethylamine group, morpholine group, 1-methylpiperazine group, cyclopropylamine group and 4-hydroxypiperidyl group. The compound can selectively inhibit the activity of acetylcholinesterase, and has the potential to develop into a drug for treating Alzheimer's disease due to the abilities of self-aggregation and the like. 1‑42 ​

本发明公开了一种基于静电驱动的双酶级联放大荧光传感器及其制备方法和应用，所述纳米酶荧光传感器由探针Azo‑Bodipy 685、胆碱氧化酶（CHO）和氯化乙酰胆碱（ACh）组成。本发明基于静电吸附原理，提出静电络合共固定化‑酶级联反应协同策略，构建一种新的静电驱动荧光传感器，利用ACh的季铵盐正电荷与CHO表面负电荷的静电作用，在特异性荧光探针Azo‑Bodipy 685表面构建纳米尺度空间限域的酶反应微环境。当有机磷农药抑制乙酰胆碱酯酶（AChE）时，氯化胆碱生成减少导致CHO催化产生的过氧化氢量下降，荧光信号强度减弱，一系列荧光强度的变化可以实现检测AChE活性和测定有机磷农药残留含量。

The invention discloses a benzofuran glycoside compound as well as a separation and extraction method, a pharmaceutical composition and application thereof. The compound 1 is separated from an eupatorium chinense extract. And separating and purifying chemical components of the n-butyl alcohol part of the eupatorium chinense root to obtain the benzofuran compound. The inhibition activity of target cyclooxygenase-2 of inflammatory related diseases of all the separated compounds 1 is tested, and the compounds 1 have good inhibition activity on COX-2 enzyme and have good binding energy with target protein. In addition, the compound also has a good NLRP3-related inhibition effect, can be independently used or combined with other medicines to regulate NLRP3-related proteins, and is used for treating NLRP3-related mediated diseases and / or diseases and preparing medicines for preventing or treating the diseases or diseases. The compound 1 also has a good inhibition effect on acetylcholin esterase, and can be used for treating Alzheimer's disease, myasthenia gravis, Parkinson's disease and other diseases.

This application relates to the field of medicinal chemistry, specifically disclosing a piperidine derivative, a pharmaceutical composition, its uses, and a method for preparation. The piperidine derivative has the structure of general formula (I). This piperidine derivative can significantly improve the learning and memory abilities of Aβ-induced Alzheimer's disease model rats and effectively reduce acetylcholinesterase activity in brain tissue, providing a potential candidate for the development of novel Alzheimer's disease treatments.

The application discloses a kind of photoactivation manganese ion bridging type deoxyribozyme probe preparation and its application in Cistanche inhibiting acetylcholinesterase activity.The probe is pSD@MnO2 Composite structure, which is composed of manganese dioxide nanosheet and functional nucleic acid assembly adsorbed on its surface, the assembly includes S chain labeled with fluorescence / quenching group and containing deoxyribozyme cleavage site, and pD chain partially hybridized with S chain and containing photocleavage site.Its preparation includes nucleic acid probe assembly, MnO2 Nanosheet complexing and purification steps.When applied, AChE catalytic product decomposes MnO2, releases Mn 2+ And nucleic acid chain; after centrifugation, pD chain is cut by ultraviolet light activation trigger, and released DNAzyme is cut under the assistance of Mn 2+ It produces amplified fluorescence signal; by comparing signal change before and after adding inhibitor, inhibition activity can be quantitatively evaluated.The application combines photocontrol activation with enzyme cascade amplification, realizes high sensitivity and operation synchronism of detection process, and is especially suitable for high-throughput rapid screening of AChE inhibitor in Cistanche and other natural products.

The invention discloses a 2, 4-dihydroxy-6-benzyl formic acid polyol ester compound in a gold wire brush and an extraction and separation method and application thereof, the structure of the 2, 4-dihydroxy-6-benzyl formic acid polyol ester compound adopts 1H-NMR (nuclear magnetic resonance), 13C-NMR (nuclear magnetic resonance) and two-dimensional nuclear magnetic spectrum analysis methods to determine that compounds I-1-I-3 are new compounds, and the 2, 4-dihydroxy-6-benzyl formic acid polyol ester compound has the following structure. The 2, 4-dihydroxy-6-benzyl formic acid polyol ester compound can effectively inhibit the activity of acetylcholin esterase (AChE), so that the compound disclosed by the invention can be used as a lead compound for resisting the acetylcholin esterase, and a material basis and a theoretical basis are provided for developing new drugs and new components.

This invention discloses a biosensor membrane for the detection of organophosphorus pesticides, comprising a base membrane on which multiple glucose oxidase catalytic modules and multiple acetylcholinesterase catalytic modules are loaded. Each glucose oxidase catalytic module includes a first carrier immobilized on the base membrane and glucose oxidase loaded thereon. Each acetylcholinesterase catalytic module includes a second carrier immobilized on the base membrane and acetylcholinesterase loaded thereon. The weight ratio of glucose oxidase to acetylcholinesterase is 1:1 to 1:4. This invention reveals the structure-activity relationship of enzyme ratio-enzyme load synergistic regulation of sensing interface performance. By establishing a competitive inhibition advantage through changing the enzyme ratio, the enzyme load is optimized to achieve a balance between high activity and high mass transfer, thereby synergistically improving signal change and inhibition rate, achieving highly sensitive detection of organophosphorus pesticides. The biosensor membrane provided by this invention is suitable for the detection of various organophosphorus pesticides in food (especially crops and dairy products).

This invention belongs to the field of pharmaceutical technology and relates to eight glycoside compounds extracted and isolated from the dried leaves of *Picrasma quassioides* Benn, a plant belonging to the genus *Picrasma* in the family Simaroubaceae. The compounds were obtained using chromatographic methods including silica gel chromatography, HP20 column chromatography, ODS column chromatography, and HPLC. This invention also relates to the acetylcholinesterase inhibitory activity of compounds 1-8 and their pharmaceutical uses. The preparation method of this invention is simple, reproducible, and of high purity.

The application discloses a FRET fluorescent probe with dual response to acetylcholinesterase and viscosity, a preparation method and application thereof, wherein the structure of the FRET fluorescent probe is shown in the following formula: The fluorescent probe is based on a fluorescence resonance energy transfer (FRET) mechanism, responds to acetylcholinesterase (AChE) at a short wave of 520 nm, and the detection limit is as low as 0.104 U / L, and responds to viscosity at a long wave of 680 nm, and the wavelength difference is as high as 160 nm, and the dual-channel response has no crosstalk. Cell toxicity test shows that the probe has good biocompatibility, and confocal fluorescence microscopic imaging experiment shows that the probe has good light stability for cells, has the ability of simultaneously monitoring the concentration change of AChE in cells and the viscosity fluctuation of cells, and is suitable for confocal fluorescence imaging for tracing the concentration change of AChE in cells and the viscosity fluctuation.

The invention discloses a high-flux toxicity pre-evaluation substitution method based on an acetylcholin esterase target. By integrating an SH-SY5Y nerve cell endogenous AChE and recombinant exogenous AChE dual detection system and relying on an automatic screening system constructed by integrating core equipment such as an automatic pipetting workstation, a constant-temperature incubator and a multifunctional microplate reader, a high-flux toxicity pre-evaluation substitution method based on an AChE target is successfully established and is used for evaluating the neurotoxicity of a compound, and the method has the advantages of high sensitivity, high sensitivity, high sensitivity and the like. The method solves the limitation that a single screening method in the prior art cannot evaluate factors such as target inhibition, cell permeability and metabolic transformation at the same time, and the method is simple, convenient, rapid, high in accuracy and suitable for large-scale primary screening of the AChE inhibitor and early warning of neurotoxicity risks.

The invention provides a phenolic compound with an effect of accelerating alcohol metabolism and a preparation method of the phenolic compound. The structural formula of the polyphenol compound 1-(2-hydroxyl, 4-O-glucosylphenyl)-2-(4-hydroxyphenyl) ethanone is as shown in formula (I), and the polyphenol compound 1-(2-hydroxyl, 4-O-glucosylphenyl)-2-(4-hydroxyphenyl) ethanone is separated from radix puerariae. In-vitro pharmacological experiments prove that the compound 1-(2-hydroxyl, 4-O-glucosylphenyl)-2-(4-hydroxyphenyl) ethanone has good acetylcholin esterase inhibitory activity, and the EC50 value of the compound 1-(2-hydroxyl, 4-O-glucosylphenyl)-2-(4-hydroxyphenyl) ethanone is 1.41 + / -0.12 mM, which indicates that the compound 1-(2-hydroxyl, 4-O-glucosylphenyl)-2-( The polyphenol compound 1-(2-hydroxyl, 4-O-glucosylphenyl)-2-(4-hydroxyphenyl) ethanone is expected to be used as a lead compound to develop a novel alcohol effect dispelling and liver protecting medicine.

The application belongs to the technical field of biosensing and specifically relates to a biosensor for detecting active acetylcholinesterase and application thereof. The biosensor comprises nanotubes with nanometer apertures at tips for generating current blocking signals; and a ring bundle DNA tetrahedron carrier for loading a solution containing active acetylcholinesterase to be detected, wherein the ring bundle DNA tetrahedron carrier is TDN1 or TDN4, the sequences of the four DNA single strands in TDN1 are shown as SEQ ID NO. 1 to SEQ ID NO. 4, and the sequences of the four DNA single strands in TDN4 are shown as SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 5 and SEQ ID NO. 6. The application utilizes a closed-loop DNA tetrahedron nanostructure anchored at two ends of an aptamer to generate a difference in current signals in a nanometer hole before and after the active AChE is combined, so that high-sensitivity and high-selectivity detection of the active AChE at a single-molecule level is achieved.

The present application relates to the technical field of medicine, and specifically discloses a mangrove sediment mud-derived fungal fermentation extract, a preparation method and application thereof. Pseudallescheria angusta SYSU-M4 is inoculated into a liquid culture medium to obtain a spore liquid; the preservation number of the mangrove sediment mud-derived fungus is GDMCC No: 66263; (2) the spore liquid is inoculated into a solid culture medium to obtain a fermentation product; (3) the fermentation product is extracted by an extraction solvent, extracted by an extraction solvent, and separated by a separation solvent, and a component with a detection relative migration value of 0.4 in thin layer chromatography is collected, and the fermentation extract is obtained. The fermentation extract has obvious acetylcholinesterase and NO inhibitory activity, and has a good application prospect in the preparation of anti-inflammatory drugs and anti-inflammatory health products. The preparation method is simple in operation and suitable for large-scale production.

The present application relates to nematode disease prevention and treatment agent technical field, especially to a kind of pine wood nematode disease prevention and treatment agent and preparation method thereof, by weight parts, including the following components: sophocarpidine 10-30 parts;Azadirachtin 5-20 parts;Turpentine 40-70 parts;Emulsifier 5-15 parts;Efficiency agent 1-5 parts;Stabilizer 0.5-3 parts, the synergistic effect of sophocarpidine and azadirachtin constitutes the core of the present application;Quinolone ring and pyridine ring structure in sophocarpidine molecule endow it with strong insecticidal activity, mainly by inhibiting acetylcholinesterase to interfere with the nerve conduction of nematode;And the complex triterpenoid structure of azadirachtin can interfere with the hormone system of nematode, inhibit its growth and development and reproduction.The two different mechanisms of action form complementation at molecular level, both can quickly kill nematode, and can long-term inhibit its population growth;Second, turpentine as natural terpene compound, the permeability of other active ingredients is enhanced.

The application discloses a benzofuran glycoside compound, a separation and extraction method thereof, a pharmaceutical composition and application, and the compound 1 is separated from a macleaya cordata extract. A benzofuran compound is obtained by separating and purifying a n-butanol part chemical component of a macleaya cordata root. The inhibitory activity of all separated compounds 1 on a target point cyclooxygenase-2 of an inflammatory related disease is tested, the compound 1 has good inhibitory activity on COX-2 enzyme, and the compound 1 has better binding energy with the target point protein. The compound also has good NLRP3 related inhibitory effect, can be used alone or in combination with other drugs to regulate NLRP3 related proteins, is used for treating NLRP3 related mediated diseases and / or diseases, and is used for preparing drugs for preventing or treating the diseases. The compound 1 also has better inhibitory effect on acetylcholinesterase, and can be used for treating diseases such as Alzheimer's disease, myasthenia gravis and Parkinson's disease.

The application discloses a kind of Ascophyllum nodosum fucoidan ANFS and its preparation method and application in preparation for preventing and treating alzheimer's disease preparation, belong to marine biological medicine technical field.The main chain of the Ascophyllum nodosum fucoidan ANFS is composed of (1→3) connected α-L fucose residue, and branch is monosaccharide or sulfate group.Sulfated modification occurs at C-2 and C-4 of fucose residue, and its molecular weight is 15-30 kDa.The application proves by experiment, ANFS can significantly improve the motion behavior defect of zebra fish, inhibit acetylcholinesterase activity, improve catalase and glutathione peroxidase activity, reduce intracellular reactive oxygen species level, reduce brain neuron apoptosis, and effectively protect the integrity of blood-brain barrier.The polysaccharide is rich in source, and preparation process is simple, and safety is high, has wide application prospect in the development of marine medicine and functional product for treating alzheimer's disease.

This invention discloses the use of cyanidin anthocyanins or their pharmaceutically acceptable salts, esters, and solvates in the preparation of drugs for the prevention and / or treatment of neurodegenerative diseases. The cyanidin anthocyanins are selected from cyanidin-3-O-rutin glycoside, cyanidin-3-O-sophoroside, or cyanidin-3-O-xyloside. These compounds can inhibit acetylcholinesterase activity, upregulate α7 nicotinic acetylcholine receptor expression, and activate the PI3K / AKT / GSK3β signaling pathway, thereby inhibiting Tau protein hyperphosphorylation and reducing neuroinflammatory responses. Furthermore, it has been confirmed that cyanidin-3-O-rutin glycoside directly binds to acetylcholinesterase, verifying AChE as its key target, providing new natural active compounds and their mechanisms of action for the prevention and treatment of neurodegenerative diseases.

The invention discloses a biosensing membrane for organophosphorus pesticide detection, which comprises a base membrane, a plurality of glucose oxidase catalysis modules and a plurality of acetylcholin esterase catalysis modules are loaded on the base membrane, each glucose oxidase catalysis module comprises a first carrier fixed on the base membrane and glucose oxidase loaded on the first carrier, and each acetylcholin esterase catalysis module comprises a second carrier fixed on the base membrane. The acetylcholin esterase catalysis module comprises a second carrier fixed on the base membrane and acetylcholin esterase loaded on the second carrier, and the weight ratio of glucose oxidase to acetylcholin esterase is (1: 1)-(1: 4). The invention discloses a structure-function relationship of the performance of an enzyme ratio-enzyme loading quantity coordinated regulation sensing interface, and optimizes the enzyme loading quantity to pursue the balance of high activity and high mass transfer on the basis of establishing a certain competitive inhibition advantage by changing the enzyme ratio, so that the signal change and inhibition ratio are synergistically improved, and the high-sensitivity detection of the organophosphorus pesticide is realized. The biosensing film provided by the invention is suitable for detecting various organophosphorus pesticides in food (especially crops and dairy products).

The invention discloses a derivative with a benzo-nitrogen heterocyclic structure and application thereof, and relates to the technical field of new drug development, and the structure of the derivative is shown as a general formula (I). The compound can inhibit beta-amyloid protein aggregation and acetylcholin esterase activity at the same time, also has excellent antioxidant and neuroprotective effects, and is mainly used for treating and preventing Alzheimer's disease and other nervous system diseases. (I).

The application provides a preparation method of metal-doped carbon dots, comprising the following steps: drying an aqueous solution of reactants containing ethylenediaminetetraacetate, Cu 2+ and Fe 3+ , high-temperature pyrolysis, acid washing, water washing, methanol purification and filtering, and drying at 60 DEG C to obtain the metal-doped carbon dots. The phenol measurement steps provided by the application are as follows: mixing a sample solution with a buffer solution, a 4-aminoantipyrine solution and a carbon dot solution, incubating to obtain a solution A, and measuring the absorbance of the solution A at 509 nm for quantitative analysis. The measurement steps of dimethoate provided by the application are as follows: mixing a sample solution with a acetylcholinesterase solution, incubating for half an hour, adding a acetylthiocholine chloride solution and incubating for another half an hour, adding a solution A, and incubating for half an hour before fluorescence measurement. The synthesis method provided by the application is simple, the detection method has high sensitivity and small interference, and is suitable for simultaneous detection of phenol and dimethoate in actual samples.

The present application relates to a kind of steroid compound and its application in treating neurodegenerative diseases, the steroid compound has the structure shown in formula I: The steroid compound of structure I is isolated from the whole grass of Solanum nigrum by yeast fermentation for the first time, it shows better neurotrophic factor (NGF) activity and acetylcholinesterase inhibitory activity, is expected to be developed as the drug or functional food of neurotrophic and neuroprotective function, for treating neurodegenerative diseases (such as senile dementia).

The invention relates to a piperidine alkoxy cotinine compound as well as a preparation method and application thereof. The general formula of the chemical structure of the piperidine alkoxy cotinine compound is shown as a formula (I), in the formula (I), R1 is selected from one of C1-6 alkyl, benzyl, substituted benzyl and pyridylmethyl, the substituted benzyl is benzyl of which the benzene ring is substituted by 1-4 substituents, and the substituents are respectively and independently selected from one of halogen, C1-4 alkyl, C1-4 alkoxy, N (CH3) 2, NHCOCH3 and CN; r2 is selected from H or halogen; and n is an integer selected from 1-4. The piperidine alkoxy cotinine compound or the pharmaceutically acceptable salt of the piperidine alkoxy cotinine compound has relatively strong inhibitory activity on the formation of acetylcholin esterase and A beta 1-42 oligomer, and also has anti-neuroinflammation activity at the same time.

The application discloses a fluorescent probe and a preparation method and application thereof, and belongs to the technical field of fluorescent detection and rapid detection of pesticide residues. The fluorescent probe provided by the application takes hydroxyl semi-florocyanine as a fluorescent mother nucleus and acetyl as a recognition site; an intermediate NS-OH is subjected to a substitution reaction with acetyl bromide; the obtained fluorescent probe NS-A can detect acetylcholinesterase and can also be combined with an enzyme inhibition method to detect pesticide residues in traditional Chinese medicinal materials; a standard curve is drawn by a fluorescence spectrum method to quantitatively determine the pesticides, and the result shows that the standard curve error of the pesticide chlorpyrifos is small, the pesticide standard addition recovery rate in the traditional Chinese medicinal materials is between 96.03% and 102.57%, and the detection lower limit of the pesticide can reach the required detection standard; the fluorescent probe has the advantages of low cost, high sensitivity, good selectivity and the like, and has a good application prospect.

The application belongs to the technical field of medicine, and particularly relates to a steroidal compound in medicinal fungus Shandong ganoderma, a preparation method of the steroidal compound and application of the steroidal compound in resisting Alzheimer's disease. The structures of the steroidal compounds 1-4 are shown in the following; the application further provides application of the compounds in preparing medicines for resisting Alzheimer's disease. The preparation method is simple, reproducible, the obtained compound has high purity, and has good inhibitory activity on acetylcholinesterase.

The invention discloses a method for purifying ginger polysaccharide and application thereof, and belongs to the technical field of natural product extraction. The preparation method comprises the following steps: mixing ginger powder with hot water, carrying out ultrasonic treatment, carrying out reflux extraction, filtering to obtain a crude extract, carrying out trichloroacetic acid precipitation to remove proteins, carrying out ethanol precipitation to enrich polysaccharides, carrying out dialysis according to a specific molecular weight cut-off and other key steps in sequence, and finally dissolving and freeze-drying to obtain the refined ginger polysaccharides. The ginger polysaccharide prepared by the method shows remarkable and excellent activity in the aspects of oxidation resistance, xanthine oxidase inhibition, acetylcholin esterase inhibition and the like, and a high-value raw material is provided for precise development of the ginger polysaccharide in the fields of functional foods, health care products or medicines.

The present application relates to the field of microorganisms, and provides a propionibacterium acidifaciens BL8 with functions of reducing blood sugar and resisting aging, and application thereof, the propionibacterium acidifaciens BL8 is preserved in China General Microbiological Culture Collection Center on December 20, 2024, and the preservation number is CGMCC No.33134.The propionibacterium acidifaciens BL8 of the present application can inhibit DPP4 activity, promote INS-1 insulin secretion, inhibit acetylcholinesterase activity and superior cellulase activity.Can reduce blood sugar;Improve memory, prevent and relieve Alzheimer's disease;Promote cellulose absorption, improve constipation, and have high research and application value in reducing blood sugar and resisting aging.

The application discloses a kind of chess nan linaloe leaf flavonoids compound extraction purification method and application.It belongs to flavonoids extraction technical field.The application extracts flavonoids compound in chess nan linaloe leaf by ultrasonic wave auxiliary ethanol extraction method, and is determined by single factor test and response surface method optimization optimum extraction process;Further, macroporous adsorption resin is used to purify crude extract, and obtain purified flavonoids compound.Flavonoids compound contains many active ingredients such as acacia leaf glycoside, quercetin, sakura flower element by identification.The method of the application is high in extraction efficiency, simple in operation, environmentally friendly and safe, and the obtained flavonoids compound has significant antioxidant activity and inhibitory activity of acetylcholinesterase and alpha-glucosidase, which provides technical support for the development and utilization of chess nan linaloe leaf.

The invention discloses a preparation method of alcohol-free extracted lavender polysaccharide and application of the alcohol-free extracted lavender polysaccharide in anti-aging products. The lavender polysaccharide (LAPC) is extracted from a lavender raw material by adopting a hot water extraction method in combination with a membrane separation technology, the yield is 4.8-8.6%, the polysaccharide content is 15-30%, the uronic acid content is 15-30%, and the pH value is 6.0-6.7. An anti-aging activity experiment in an aging mouse model induced by D-galactose shows that the LAPC prepared by the invention can remarkably improve the activity of antioxidant enzyme in an aging mouse body; the cell morphology of liver and kidney tissues is effectively improved; multiple serum biochemical indexes are recovered to be normal; the expression level of proinflammatory factors is reduced; the activity of acetylcholin esterase and nitric oxide synthase in brain tissues is inhibited; and the expression of a p53 / p21 protein signal channel is down-regulated. The LAPC provided by the invention has remarkable anti-aging activity. The provided preparation method of the lavender polysaccharide is simple, convenient and efficient, does not need complex chemical reagents, and has the potential of being developed into low-toxicity and efficient anti-aging cosmetics or medicine raw materials.

Provided in the present invention are a biological enzyme preparation which rapidly reduces pesticide residues and a preparation method therefor, belonging to the technical field of pesticide residue degradation. The biological enzyme preparation which rapidly reduces pesticide residues provided in the present invention comprises the following components in parts by weight: 10-20 parts of a phytase, 10-50 parts of acetylcholinesterase, 5-10 parts of a protease, 10-20 parts of tyrosinase, 1-3 parts of a Platycodon grandiflorus extract, and 1-3 parts of an Aloe vera extract. Provided in the present invention is a biological enzyme preparation which rapidly and efficiently reduces pesticide residues; the biological enzyme preparation has a removal rate of 95% or higher with respect to organophosphorus pesticides on fruits and vegetables and a removal rate of 70% or higher with respect to pyrethroid pesticides on fruits and vegetables; moreover, the biological enzyme preparation provided in the present invention has a simple formulation, is easy to wash off, and does not form a secondary residue.