The utility model relates to the technical field of isocarbophos detection card accessory devices, in particular to a rapid isocarbophos detection card and an isocarbophos detection kit with two groups of detection holes and detection test paper, and provides the rapid isocarbophos detection card which has the advantages that the resource waste is reduced, the detection time is shortened, and the practicability is enhanced. Comprising a first detection bottom box, a second detection bottom box, a first detection top cover, a second detection top cover, a clamping rod, a limiting block, a supporting block and a clamping rod, the first detection bottom box and the second detection bottom box are connected with the first detection top cover and the second detection top cover through clamping assemblies respectively, and the right end of the first detection top cover is connected with two first sleeves; the left end of the second detection top box is connected with the second sleeve, the clamping rod is slidably sleeved with the two first sleeves and the two second sleeves, the top end of the clamping rod is connected with the limiting block, the top end of the supporting block is connected with the clamping rod, and a clamping hole is formed in the bottom end of the clamping rod.