1345 results about "Choline" patented technology

The invention provides a composition beneficial to improvement of brain cognition, memory and sleep and prevention and improvement of Alzheimer's disease, and belongs to the technical field of health food. The composition comprises L-alpha-glycerophosphorylcholine (GPC), phosphatidylserine (PS), docosahexaenoic acid (DHA), tea leaf theanine (L-theanine), vitamin B groups, vitamin D3 and the like, and is prepared into a health care product, a dietary supplement, a functional food, a medical food or a nutritional supplement by adopting the existing technology. The traditional Chinese medicine composition has the effects of effectively nourishing nerve cells, improving the brain sensitivity and improving the cognitive function. The composition is particularly directed at age-related cognitive decline and neurodegenerative diseases. Repair and regeneration of nerve cells can be promoted, the transmission efficiency of neural signals is enhanced, and the overall working efficiency of the brain is improved. Daily life challenges caused by cognitive decline can be relieved, and individual life quality can be improved.

The present invention relates to a novel association or combination of active ingredients useful in the prevention and repair of neuronal damage, both centrally and peripherally. In greater detail, the present invention relates to an association or combination of i) an extract of Gastrodia elata and ii) at least one ingredient selected from the group comprising or, alternatively, consisting of citicoline, L-acetylcarnitine and, preferably, at least one vitamin of group D, and / or mixtures thereof, and / or at least one vitamin of group B, and / or mixtures thereof. Furthermore, the invention also relates to the pharmaceutical compositions and / or compositions for medical devices (Regulation (EU) 2017 / 745) and / or nutraceutical and / or food compositions comprising that association and to their use in therapy, in particular in the protection of the functionality of the nervous system and in the prevention and / or treatment of central and peripheral neuropathies.

The invention relates to the technical field of biological extraction, in particular to a green synergistic extraction system and an extraction method and application thereof.The green synergistic extraction system comprises a deep eutectic solvent system, an enzymolysis system and an ultrasonic-assisted extraction system, and the deep eutectic solvent system comprises a DES solvent; the DES solvent is composed of a hydrogen bond acceptor and a hydrogen bond donor, and the hydrogen bond acceptor is choline, choline chloride or L-proline; the hydrogen bond donor is lactic acid, urea or ethylene glycol; enzyme preparations in the enzymolysis system are at least two of cellulase, pectinase and beta-glucosidase; the problems that a traditional organic solvent is high in toxicity and poor in biodegradability and synthetic deep eutectic solvent raw materials are non-renewable are solved, a green synergistic extraction system which is environmentally friendly, low in cost and excellent in performance is developed, the mass transfer efficiency and the extraction yield are improved, the used DES and enzyme preparations can be recycled, and the cost is reduced while the environment is protected.

The invention relates to a recombinant pichia pastoris strain capable of producing astaxanthin at high yield as well as a construction method and application of the recombinant pichia pastoris strain. The recombinant pichia pastoris strain is obtained by expressing ATP (adenosine triphosphate) citrate lyase (ACL) and acetyl-CoA synthase (ACS) or phosphoketolase (PK) and phosphotransacetylase (PTA), choline kinase (CK), inositol polyphosphate kinase (IPK) and vitreoscilla hemoglobin (VHB) in host bacteria. Wherein the host bacterium is a pichia pastoris gene modified strain PP-LC2. The astaxanthin production performance of the recombinant strain is verified on the basis of comparison of precursor supply pathways, IUP pathway construction and improvement of the oxygen supply capacity of the engineering strain, and the astaxanthin production capacity of the pichia pastoris is further improved. The construction method of the recombinant pichia pastoris is simple, the synthesis of astaxanthin can be better promoted, the engineering strain can efficiently synthesize the astaxanthin by utilizing methanol through amplification fermentation of a 5L fermentation tank, and industrial production is facilitated.

The present invention discloses a preparation method of yellow azoxy and azo stilbene dyes. Using 4-nitrotoluene-2-sulfonic acid as a raw material and water as a reaction solvent, in the presence of choline hydroxide, dye yellow 11 is obtained through a condensation reaction. The molecular structure of choline hydroxide contains a quaternary ammonium salt group and a hydroxyl group. The hydroxyl group provides additional hydrophilicity, which helps to improve the solubility and dispersibility of the dye in an aqueous medium and enhance the affinity between the dye and the fiber. Choline hydroxide can effectively provide the alkaline environment required for the reaction, promote the synthesis reaction of the dye, and better control the selectivity and yield of the reaction. Since the choline hydroxide molecule contains a hydroxyl group, it can form hydrogen bonds with the sulfonic acid groups in the dye molecule, enhance the stability between dye molecules, and improve the light fastness and acid and alkali resistance of the dye. Using choline hydroxide not only optimizes the reaction conditions, improves the purity and yield of the dye, but also significantly improves the performance of dye yellow 11.

The invention discloses a brassica rapa imago feed and a preparation method thereof, and belongs to the technical field of insect breeding. The Eye spot Brassica rapa adult feed comprises 11 to 15 parts of casein, 1 to 3 parts of Webster salt, 4 to 6 parts of cane sugar, 9 to 12 parts of cellulose powder, 0.2 to 0.4 part of L-cysteine, 0.2 to 0.3 part of choline chloride, 0.08 to 0.1 part of inositol, 0.325 to 0.4 part of composite vitamin, 2 to 3 parts of a nutritional ingredient ethanol solution, 12 to 14 parts of a nutritional ingredient aqueous solution, 1 to 2 parts of honey, 2 to 3 parts of agar powder, 0.03 to 0.04 part of cholesterol, 33 to 67 parts of apple jam, 0.3 to 4 parts of royal jelly and 80 to 120 parts of water. In addition, the invention also provides a preparation method of the feed for the Eye spot Brassica rapa imagoes. The feed for the Eye spot Brassica rapa adults can prolong the life of the adults and improve the egg laying amount at the same time.

The invention provides an inhalable tanshinone IIA lipid nanocrystal as well as a preparation method and application thereof, and the inhalable tanshinone IIA lipid nanocrystal is mainly formed by entrapping tanshinone IIA by using dipalmitoyl phosphatidylcholine DPPC and dipalmitoyl phosphatidyl ethanolamine DPPE as mixed phospholipids in the presence of a stabilizer. Compared with the prior art, the inhalable tanshinone IIA lipid nanocrystal disclosed by the invention has the advantages that by improving the physicochemical property and drug release behavior of TA, the lung retention volume of TA after aerosol inhalation is obviously increased, the retention time of TA in the lung is prolonged, and the risk of toxic and side effects caused by whole-body escape of drugs is reduced; the treatment effect on idiopathic pulmonary fibrosis is improved, and the compliance of a patient is improved.

The invention belongs to the technical field of biology, and particularly relates to an SLC44A1 gene and / or SLC44A5 gene knockout animal model as well as a construction method and application thereof. The construction method of the animal model comprises the following steps: deleting exon regions from the fourth exon region to the thirteenth exon region of the SLC44A1 gene and / or the second exon region of the SLC44A5 gene in mouse brain oligodendroglia cells in a high-efficiency manner by utilizing gene editing and a Cre-loxP recombinase system, so as to realize the knockout of a target gene. The animal model constructed by the invention can be used for researching the regulation mechanism of the choline transporters (SLC44A1 and SLC44A5) on myelin sheath formation, deeply knowing the biological functions of the choline transporters (SLC44A1 and SLC44A5), simulating the occurrence and development processes of human related diseases, revealing the pathogenesis of the related diseases, providing a new thought for prevention and treatment of brain myelin sheath dysplasia, and providing a new foundation for the prevention and treatment of brain myelin sheath dysplasia. And an effective drug or a treatment mode aiming at the disease is screened by utilizing a gene knockout animal model, so that a new choice is provided for clinical treatment of the disease.

The invention relates to the technical field of bioengineering, and discloses a genetically engineered bacterium and a method for producing icaritin through whole-cell catalysis. By constructing double plasmids, choline kinase, isopentenyl phosphokinase, flavonoid 3-hydroxylase, flavonol synthase, isopentenyl transferase and oxymethyltransferase are simultaneously overexpressed in escherichia coli, so that a genetically engineered bacterium is constructed, and the engineering bacterium is used as a whole-cell catalyst to prepare a whole-cell engineering bacterium. The naringenin and the 3-methyl-2-butene-1-ol are used as substrates to be converted into the icaritin in a whole-cell catalysis mode, only one-step reaction is needed, the method is simple, rapid and high in efficiency, other chemical substances do not need to be additionally added in the conversion process, the cost is reduced, pollution to the environment is relieved, and the method is suitable for industrial production. And the biological production from low-price naringenin to high-added-value icaritin is realized.

The invention provides a method for preparing high-purity phosphatidylserine by a batch enzyme catalysis method based on dynamic choline removal, and belongs to the technical field of enzymatic reaction. According to the method disclosed by the invention, in a reaction system for preparing phosphatidylserine, a byproduct choline is selectively adsorbed by adopting resin. Choline in a reaction system is removed in real time through the integrated adsorption separation module, the enzyme inhibition problem is solved, and meanwhile, the PC conversion rate is larger than or equal to 98% and the PS purity is larger than or equal to 96% in combination with the immobilized enzyme circulation technology; the enzyme can be reused for more than or equal to 20 times (activity retention is more than or equal to 90%); and the choline residual concentration is less than or equal to 0.5 mM (an inhibition threshold value or less).

The invention provides a medical hydrophilic coating and a preparation method and application thereof.The coating comprises a substrate layer and a functional layer, the substrate layer is arranged on the surface of a medical base material, the functional layer is arranged on the surface of the side, away from the medical base material, of the substrate layer, and the substrate layer comprises a substrate modification material containing a first reactive functional group; the functional layer comprises a phosphorylcholine compound containing a second reactive functional group; the substrate layer and the functional layer are connected through a chemical bond formed by the first reactive functional group and the second reactive functional group; the substrate modification material contains a third reactive functional group, and the substrate layer is connected with the medical base material through a chemical bond formed by the third reactive functional group and hydroxyl or amino on the surface of the medical base material; when the first reactive functional group is an azide group, the second reactive functional group is an alkynyl group; or when the first reactive functional group is the alkynyl group, the second reactive functional group is the azide group. Chemical bonds are formed through azido and alkynyl, multiple chemical reactions are not needed, and the uniformity of the coating is improved.

The invention relates to the technical field of bacterium enrichment nutrient solutions, in particular to a bacterium enrichment nutrient solution for fermentation and a preparation method of the bacterium enrichment nutrient solution. The culture medium is prepared from the following raw materials in parts by weight: maltodextrin, crystalline glucose, low-pigment yeast extract powder, refined soy peptone, dipotassium phosphate, monopotassium phosphate, magnesium sulfate heptahydrate, sodium chloride, phytic acid, L-ascorbic acid, manganese sulfate monohydrate, biotin, thiamine hydrochloride and choline chloride. Through a double-carbon-source structure, maltodextrin and crystalline glucose cooperate, metabolic oscillation is avoided, and the energy utilization rate is increased; the low-pigment yeast extract powder and the refined soy peptone are complementary, so that the rapid growth requirement of thalli is met; phytic acid is chelated with metal ions to prevent precipitation, L-ascorbic acid is used for resisting oxidation, and biotin and thiamine activate a coenzyme pathway to realize accurate regulation and control of a metabolic network.

The invention belongs to the technical field of agricultural biology, and discloses a method for inducing curcuma wenyujin tissue culture seedling test-tube micro rootstocks, which comprises the following steps: inoculating terminal bud cluster proliferation buds of curcuma wenyujin obtained by conventional tissue culture into a rootstock induction culture medium for rooting culture for 30 days to obtain tissue culture seedlings with rootstocks, the rhizome induction culture medium is prepared from 1 / 2MS, 0.1-1.0 mg / L of NAA, 30 g / L of cane sugar, 1-3 mg / L of choline chloride and 1-3 mg / L of compound sodium nitrophenolate, the pH is 5.8, and the weight ratio of choline chloride to compound sodium nitrophenolate is 1: (0.3-2). According to the method, the formation of the curcuma wenyujin test-tube micro rhizomes is induced in vitro, the sterile micro rhizomes are directly transplanted, and the micro rhizome artificial seeds are manufactured for sowing, so that a green seedling hardening and temporary planting link is omitted, and the seedling loss caused by strict requirements of green seedlings on humidity is reduced, and the test-tube micro rhizome seedlings are beneficial to storage and long-distance transportation; therefore, the seedling breeding efficiency of the rhizome plants is improved.

The present disclosure provides unshielded lipid nanoparticles and a process that enables the production of such unshielded lipid nanoparticles, thereby overcoming previous challenges of making particles without causing aggregation thereof. The lipid nanoparticles comprise a nucleic acid cargo molecule; a sterol or a derivative thereof present at a content of at least 12 mol%; a neutral lipid, such as a phospholipid having a choline head group present at a content of between 22 mol% and 65 mol%; and an ionizable cationic amino lipid present at a content of between 15 mol% and 45 mol%; wherein the lipid nanoparticle is non-sterically stabilized with a hydrophilic polymer-lipid conjugate, or otherwise unshielded and wherein each mol% content is relative to total lipid present in the lipid nanoparticle.

The invention provides a lignin extraction-sulfonic acid modification integrated preparation method based on eutecticevaporate solvent coupling microwaves, lignin sulfonate and application, and belongs to the technical field of oilfield chemistry. The preparation method comprises the following steps: mixing choline chloride, epichlorohydrin and 4-hydroxybenzenesulfonic acid, heating and stirring to obtain a deep eutectic solvent ternary system; the method comprises the following steps: adding biomass powder into a deep eutectic solvent ternary system, and reacting under a microwave condition; after the reaction is completed, filtering, and adjusting the pH value of the obtained filtrate to be neutral to obtain a mixed solution; and then adding an organic solvent into the obtained mixed solution for precipitation, filtering, washing and drying to complete lignin extraction-sulfonic acid modification. According to the method, in-situ grafting of sulfonic acid functional groups is realized in the lignin separation process through the ternary eutectic solvent in combination with microwave-assisted extraction, so that sulfonation modification of lignin is completed. After sulfonation modification, the obtained sulfonated lignin is applied to the drilling fluid, and the viscosity reduction effect can be achieved.

The present disclosure provides unshielded lipid nanoparticles and a process that enables the production of such unshielded lipid nanoparticles, thereby overcoming previous challenges of making particles without causing aggregation thereof. The lipid nanoparticles comprise a nucleic acid cargo molecule; a sterol or a derivative thereof present at a content of at least 12 mol %; a neutral lipid, such as a phospholipid having a choline head group present at a content of between 22 mol % and 65 mol %; and an ionizable cationic amino lipid present at a content of between 15 mol % and 45 mol %; wherein the lipid nanoparticle is non-sterically stabilized with a hydrophilic polymer-lipid conjugate, or otherwise unshielded and wherein each mol % content is relative to total lipid present in the lipid nanoparticle.

The metabolic marker can be used for early diagnosis of heart failure, has the advantages of high sensitivity, rapidness, convenience and accurate and reliable result, provides a basis for clinical decision, provides a certain basis for subsequent fundamental research and clinical research, and has potential application and research values. The metabolic marker comprises one or more of 1-(4-nitrophenyl) piperidine, sphingolipid d19: 3 / 23: 0, phosphatidyl ethanolamine 16: 1e / 22: 5, phosphatidyl ethanolamine 16: 1e / 22: 6, sphingolipid d14: 0 / 22: 1, 3-phosphoglyceric acid, phosphatidylcholine 15: 0 / 20: 4, phosphatidylcholine o-16: 1 / 18: 0, phosphatidylcholine 14: 0e / 20: 1, phosphatidylcholine 17: 0 / 18: 5, betaine, phosphatidylcholine 15: 0 / 18: 2 and phosphatidylcholine 18: 5e / 20: 4.

The invention belongs to the technical field of medicines, and particularly relates to a composition for improving obesity and promoting cardiovascular health as well as a preparation method and application thereof. The composition is prepared from the following components in parts by weight: 5 to 20 parts of glycine betaine, 1 to 5.5 parts of choline chloride, 0.5 to 4 parts of L-theanine, 1 to 15 parts of alpha-ketoglutarate calcium, 0.5 to 3 parts of black ginger extract, 0.05 to 0.25 part of black pepper extract, 0.5 to 3 parts of ginkgo leaf extract and 1 to 6 parts of black garlic juice powder. According to the invention, the eight components are selected and are scientifically combined in an optimal quantity ratio range, and the prepared composition can better reduce the inflammation level of a body, regulate abnormal blood fat, improve the lipid metabolism disorder state and effectively reduce impulsive diet behaviors, so that comprehensive improvement of obesity and cardiovascular related risks is realized, and the health-care effect of a human body is improved. The body is effectively assisted to maintain metabolic health.

The invention discloses a method for extracting active ingredients of traditional Chinese medicinal materials based on ultralow-temperature crushing and deep eutectic solvent synergy, a tableted candy for dispelling effects of alcohol and protecting liver and a preparation method of the tableted candy. The ultralow-temperature crushing and deep eutectic solvent synergy extraction technology is combined, and the active ingredients of the traditional Chinese medicinal materials with homology of medicine and food are extracted at low temperature; the method comprises the following steps: firstly, quickly freezing a raw material by using liquid nitrogen and mechanically crushing the raw material to improve the cell wall breaking rate, then stirring and mixing a deep eutectic solvent based on choline chloride and glycerol with water to reduce the viscosity of the solvent for extraction, and carrying out ultrasonic extraction, filtration, macroporous adsorption resin recovery and other steps to obtain a high-purity extract. According to the preparation method, the extraction rate of active substances in the traditional Chinese medicinal materials is increased, and extracts of multiple medicinal and edible Chinese herbal medicines such as lucid ganoderma, radix puerariae, hawthorn and olive are compounded into the tablet candy, so that liver injury caused by alcohol is effectively improved, and side effects caused by long-term administration of medicines can be effectively avoided.

Methods for treating Wilson Disease with bis-choline tetrathiomolybdate therapy are provided. The methods may include administering 15 mg or between 30 and 90 mg of bis-choline tetrathiomolybdate once daily to a patient exhibiting NCCcorrected, alanine aminotransferase (ALT), hemoglobin, platelets, or neutrophils levels meeting specified criteria. The methods may include modifying treatment by decreasing or increasing the daily dose of bis-choline tetrathiomolybdate or discontinuing treatment for a period of time.

The present invention discloses a biomass-based ionic liquid-modified carbon quantum dots, a preparation method thereof, and a composite lubricant. The preparation method of the biomass-based ionic liquid-modified carbon quantum dots uses biomass raw materials, choline chloride, and amino acids to prepare carbon quantum dots and choline amino acid ionic liquid. The choline amino acid ionic liquid is used to modify the carbon quantum dots to produce a biomass composite lubricant. This uses renewable biomass resources instead of traditional petrochemical resources, is environmentally friendly, can be decomposed by nature, achieves clean production, and eliminates environmental pollution. The CQDs / ILs composite lubricant can function as "nanoballs," thereby improving its load-bearing capacity and reducing wear. In addition to having good lubricating properties, the choline amino acid ionic liquid also has good solubility. Modifying the carbon quantum dots with the choline amino acid ionic liquid not only improves the dispersibility of the carbon quantum dots but also further enhances their tribological properties.

The invention relates to the technical field of biological pharmacy, and discloses a QS-21 saponin adjuvant as well as a preparation method and application thereof, the adjuvant is a composite liposome and comprises a liposome skeleton composed of distearoyl phosphatidylcholine and cholesterol, and QS-21 saponin, monophosphoryl lipid A and a local anesthetic are jointly entrapped in the liposome skeleton. The problem that high reactogenicity and immunogenicity of potent adjuvants are difficult to consider at the same time is solved, the immunostimulation component and the pain inhibition component are jointly entrapped in the same nano-carrier, collaborative delivery in injection local is achieved, and therefore pain and swelling caused by the adjuvants are accurately inhibited. The co-entrapment structure avoids the potential inhibition effect of the free anesthetic on the immune system, the potent body fluid and cellular immune enhancement activity of the adjuvant is completely reserved, and a new technical scheme is provided for developing vaccines with high safety and strong immune efficacy.

The invention discloses diglyceride prepared by an enzymic method as well as a preparation method and application thereof, and belongs to the technical field of deep processing of grease. The method comprises the following steps: adding fatty acid into a choline chloride-glycerol-urea ternary eutectic solvent, uniformly mixing, and then adding candida antarctica lipase B for esterification reaction to obtain a reaction mixture; separating the reaction mixture, and recovering an upper-layer oil phase; and separating and purifying the upper-layer oil phase to obtain the diglyceride. According to the method, the reaction rate is high, the selectivity of the diglyceride in the esterification process is high, the yield of the diglyceride can reach 65% or above by reacting for 1 h, the selectivity of the diglyceride is 75% or above, and the purity of the separated and purified diglyceride is 90% or above; in addition, the method is simple to operate, and the reaction product and the eutectic solvent can be separated through simple centrifugation after the reaction is finished; in addition, the monoglyceride content in the product is low, and the subsequent separation and purification difficulty is greatly reduced.

The invention belongs to the field of pharmaceutical preparations, and particularly relates to nitrogen hydrochloride # imgabs0 # stine eye drops and a preparation process thereof, the nitrogen hydrochloride # imgabs0 # stine eye drops comprise 0.05% of nitrogen hydrochloride # imgabs1 # stine, 0.2-0.3% of hydroxypropyl methylcellulose, 0.1-0.2% of edetate disodium, 0.12-0.15% of an isoosmotic adjusting agent, 0.04-0.07% of a preservative and 0.005-0.0075% of choline aromatic folic acid; the nitrogen hydrochloride # imgabs2 # stine eye drops prepared by the preparation method disclosed by the invention have the advantages that the adsorption of nitrogen hydrochloride # imgabs3 # stine on a low-density polyethylene bottle is well inhibited under the condition of visible light, and the reduction of the concentration of nitrogen hydrochloride # imgabs4 # stine in the eye drops is remarkably prevented, so that the medication safety is improved. Besides, the added cholinofolic acid and hydroxypropyl methylcellulose have small irritation to eyes under a specific ratio, and can also have a large enough contact angle with the eyes of rabbits at high temperature, so that the retention property of the eye drops on the eyes can be improved, and the drug effect can be favorably exerted.

The present invention belongs to the technical field of fly ash resource recycling and utilization, and relates to a method for the synergistic extraction of aluminum, lithium, gallium, and scandium from fly ash. The method comprises the following steps: (S1) pretreatment: adding fly ash to an aqueous solution of an inorganic base, subjecting it to microwave irradiation, and then pressurizing and activating it to obtain a pretreated product; (S2) graded acid leaching: subjecting the pretreated product to primary acid leaching in hydrochloric acid to obtain leachate I and a Si-rich residue I; subjecting the Si-rich residue I to secondary acid leaching in a mixed solution of hydrochloric acid and choline chloride-urea to obtain leachate II and a Si-rich residue II; and (S3) separation and purification: comprising selective adsorption of Ga / Sc / Fe by ion exchange resin, Ca removal and Al / Li separation, step-by-step resin elution, Ga / Sc extraction and separation, Ga stripping, and electrolysis. The present invention achieves efficient synergistic extraction and full resource utilization of aluminum, lithium, gallium, and scandium from fly ash, and the entire process has low energy consumption.

The invention discloses a special ultralow-temperature preservative for blood T cells and application of the special ultralow-temperature preservative, and the special ultralow-temperature preservative comprises the following components in percentage by mass and volume: an osmotic pressure regulating system (5-10% of glycerol and 10-20% of glycolalcohol which are compounded); an antioxidant protection system (10%-20% of human serum albumin and 0.1%-1% of glutathione); a nutrition supply agent (a cell culture medium containing glucose, amino acid, vitamin and electrolyte); a pH stable buffer pair (0.5%-3% of HEPES and 0.1%-1% of sodium bicarbonate); and a cell membrane enhancer (1%-1% of cholesterol and 0.05%-0.5% of phosphatidylcholine). The pH value of the preservative is 7.2-7.4, the preservative is suitable for long-time cryopreservation of blood T cells at the ultralow temperature of-80 DEG C to-196 DEG C, and the biological activity and functional integrity of the T cells can be effectively maintained. The invention also relates to an application of the preservative in preparation of T cell preparations preserved at ultra-low temperature, and the survival rate and functional integrity of cells after cryopreservation can be obviously improved.

The invention discloses a method for biological high-density synthesis of retinaldehyde and a synthetic strain thereof. The synthetic strain is obtained by expressing ATP citrate lyase ACL, acetyl-CoA synthase ACS, choline kinase CK, inositol polyphosphate kinase IPK and vitreoscilla hemoglobin VHb in host bacteria. Wherein the host bacterium is a pichia pastoris gene modified bacterial strain PP-B2C. The retinaldehyde production performance of the recombinant strain is verified on the basis of a precursor supply pathway, an IUP pathway and a dissolved oxygen level, and the retinaldehyde production capacity of the pichia pastoris is further improved. The construction method of the recombinant pichia pastoris is simple, the synthesis of retinaldehyde can be better promoted, and the retinaldehyde can be efficiently synthesized by the engineering strain by utilizing glucose and methanol through amplification fermentation of a 5L fermentation tank, so that the recombinant pichia pastoris is beneficial to industrial production.

The invention discloses a compound environment-friendly corrosion inhibitor as well as a preparation method and application thereof. The choline ionic liquid comprises choline ionic liquid and amino acid, the choline ionic liquid is choline cations; the amino acid is one or more of cysteine and tryptophan. The compound environment-friendly corrosion inhibitor has high corrosion inhibition efficiency, and the compound structure of the compound environment-friendly corrosion inhibitor further improves the protection performance of the compound environment-friendly corrosion inhibitor through a dual adsorption mechanism of the synergistic effect of physical adsorption and chemical adsorption on the metal surface. According to the compound environment-friendly corrosion inhibitor, a full-green ionic liquid corrosion inhibition system is constructed, the environment-friendly requirement is met, and a metal protection solution can be provided for industrial acid pickling and other strong acid working conditions. Not only is the progress of a corrosion inhibition technology promoted, but also more economic and environment-friendly anti-corrosion application can be provided for actual industrial scenes.

A method for extracting lignin from lignocellulosic biomass using a non-choline chloride based natural deep eutectic solvent (NADES) formed from Citric Acid (CA) and Fructose (Fr) comprises mixing the biomass with the NADES, obtaining a lignin-NADES suspension, lyophilizing, rehydrating, and drying the suspension, and repeating the rehydration and drying steps to obtain lignin particles. The biomass used can be Ghaf biomass waste. The NADES preparation includes adding distilled water to reduce viscosity, mixing CA and Fr with distilled water in specific molar ratios of CA:Fr:distilled water of: 1:1:1, or 3:1:1, or 5:1:1, adjusting pH, stirring, and heating. The method also includes specific conditions for mixing, stirring, heating, and terminating the reaction. The lignin-NADES suspension is obtained through vacuum filtering and lyophilized by vacuum freeze-drying. The method avoids the use of acid-precipitation techniques, ensuring an efficient and environmentally friendly lignin extraction process.

The invention discloses a lipid composition for preparing extracellular vesicles, which is prepared from the following lipid substances in percentage by mass: 2 to 18 percent of phosphatidylcholine, 2 to 14 percent of phosphatidylethanolamine, 7 to 27 percent of sphingomyelin, 0.5 to 7 percent of ceramide, 1.5 to 2.5 percent of phosphatidylinositol, 30 to 70 percent of cholesterol and 5 to 8 percent of phosphatidylserine, the fatty acid carbon number of the lipid substance satisfies the following conditions: the fatty acid carbon number of phosphatidylcholine is 16-20, the fatty acid carbon number of phosphatidyl ethanolamine is 16-18, the fatty acid carbon number of sphingomyelin is 16-24, the fatty acid carbon number of ceramide is 16-24, the fatty acid carbon number of phosphatidylinositol is 16-20, the fatty acid carbon number of phosphatidylserine is 18-24, and the molar ratio of cholesterol to sphingomyelin is 4-7. The extracellular vesicles are prepared from the lipid composition and used for biomarkers or drug carriers, and efficient oral drug delivery is achieved.