1832 results about "Choline" patented technology

The invention provides a composition beneficial to improvement of brain cognition, memory and sleep and prevention and improvement of Alzheimer's disease, and belongs to the technical field of health food. The composition comprises L-alpha-glycerophosphorylcholine (GPC), phosphatidylserine (PS), docosahexaenoic acid (DHA), tea leaf theanine (L-theanine), vitamin B groups, vitamin D3 and the like, and is prepared into a health care product, a dietary supplement, a functional food, a medical food or a nutritional supplement by adopting the existing technology. The traditional Chinese medicine composition has the effects of effectively nourishing nerve cells, improving the brain sensitivity and improving the cognitive function. The composition is particularly directed at age-related cognitive decline and neurodegenerative diseases. Repair and regeneration of nerve cells can be promoted, the transmission efficiency of neural signals is enhanced, and the overall working efficiency of the brain is improved. Daily life challenges caused by cognitive decline can be relieved, and individual life quality can be improved.

The present invention relates to a novel association or combination of active ingredients useful in the prevention and repair of neuronal damage, both centrally and peripherally. In greater detail, the present invention relates to an association or combination of i) an extract of Gastrodia elata and ii) at least one ingredient selected from the group comprising or, alternatively, consisting of citicoline, L-acetylcarnitine and, preferably, at least one vitamin of group D, and / or mixtures thereof, and / or at least one vitamin of group B, and / or mixtures thereof. Furthermore, the invention also relates to the pharmaceutical compositions and / or compositions for medical devices (Regulation (EU) 2017 / 745) and / or nutraceutical and / or food compositions comprising that association and to their use in therapy, in particular in the protection of the functionality of the nervous system and in the prevention and / or treatment of central and peripheral neuropathies.

The invention belongs to the technical field of extraction of traditional Chinese medicinal materials, and relates to a preparation method of an astragalus polysaccharide extract. The method comprises the following steps: adding astragalus membranaceus powder into a deep-eutectic solvent water solution containing cellulase, carrying out ultrasonic extraction at 25-75 DEG C for 15-60 minutes, centrifuging, taking supernatant and carrying out alcohol precipitation to obtain astragalus membranaceus polysaccharide; the eutectic solvent in the eutectic solvent aqueous solution comprises one of choline chloride and glycerol, choline chloride and lactic acid, and choline chloride and urea. The astragalus polysaccharide can be efficiently extracted by adopting the method, and the method is obviously higher than a traditional water extraction method. According to the method, a natural deep-eutectic solvent method, an enzymatic extraction method and an ultrasonic-assisted extraction method are fused into an enzyme-assisted natural deep-eutectic solvent-ultrasonic-assisted extraction method for extracting the polysaccharide from the traditional Chinese medicinal material astragalus membranaceus, the extraction rate is high, the method needs short time, energy consumption is low, and the method is a high-efficiency, green and convenient extraction method for the astragalus membranaceus polysaccharide and is suitable for large-scale industrial production. The method is expected to be used as an extraction method of novel traditional Chinese medicines and other natural medicines.

The invention relates to the technical field of biological extraction, in particular to a green synergistic extraction system and an extraction method and application thereof.The green synergistic extraction system comprises a deep eutectic solvent system, an enzymolysis system and an ultrasonic-assisted extraction system, and the deep eutectic solvent system comprises a DES solvent; the DES solvent is composed of a hydrogen bond acceptor and a hydrogen bond donor, and the hydrogen bond acceptor is choline, choline chloride or L-proline; the hydrogen bond donor is lactic acid, urea or ethylene glycol; enzyme preparations in the enzymolysis system are at least two of cellulase, pectinase and beta-glucosidase; the problems that a traditional organic solvent is high in toxicity and poor in biodegradability and synthetic deep eutectic solvent raw materials are non-renewable are solved, a green synergistic extraction system which is environmentally friendly, low in cost and excellent in performance is developed, the mass transfer efficiency and the extraction yield are improved, the used DES and enzyme preparations can be recycled, and the cost is reduced while the environment is protected.

1H-NMR spectroscopic molecular markers are provided for identifying medical risk signatures such as SARS-CoV-2 infection, acute inflammation, or a cardiovascular risk condition. The markers use a combination of NMR intensity signals, including a Glyc signal from at least one N-acetyl (—NCOCH3) glycoprotein and an SPC signal from a choline head group (+N—(CH3)3) of a supramolecular phospholipids cluster (SPC) present in HDL and LDL lipoprotein subfractions. The Glyc signal is in a chemical shift region from #=2.00 ppm to #=2.20 ppm, and includes signals GlycA (2.00 ppm to 2.09 ppm) and GlycB (2.09 ppm to 2.2 ppm). The SPC signal is in a chemical shift region from #=3.20 ppm to #=3.30 ppm, and includes signals SPC1 (3.2 ppm to 3.235) ppm, SPC2 (3.235 ppm to 3.26 ppm), and SPC3 (3.26 ppm to 3.3 ppm). A system for identifying the markers is also provided.

The invention relates to a recombinant pichia pastoris strain capable of producing astaxanthin at high yield as well as a construction method and application of the recombinant pichia pastoris strain. The recombinant pichia pastoris strain is obtained by expressing ATP (adenosine triphosphate) citrate lyase (ACL) and acetyl-CoA synthase (ACS) or phosphoketolase (PK) and phosphotransacetylase (PTA), choline kinase (CK), inositol polyphosphate kinase (IPK) and vitreoscilla hemoglobin (VHB) in host bacteria. Wherein the host bacterium is a pichia pastoris gene modified strain PP-LC2. The astaxanthin production performance of the recombinant strain is verified on the basis of comparison of precursor supply pathways, IUP pathway construction and improvement of the oxygen supply capacity of the engineering strain, and the astaxanthin production capacity of the pichia pastoris is further improved. The construction method of the recombinant pichia pastoris is simple, the synthesis of astaxanthin can be better promoted, the engineering strain can efficiently synthesize the astaxanthin by utilizing methanol through amplification fermentation of a 5L fermentation tank, and industrial production is facilitated.

The invention provides a nano photosensitizer modified by outer membrane vesicles of genetically engineered bacteria as well as a preparation method and application of the nano photosensitizer. The nano photosensitizer is a nano material with the particle size of 80-120 nm, and the nano photosensitizer modified by the genetically engineered bacterium outer membrane vesicle comprises dipalmitic acid phosphatidylcholine (DPPC), distearoyl phosphatidylcholine (DSPC), dioleoyl lecithin (DOPC), cholesterol, a small molecule dye and CD47nb-OMV membrane protein. The chemical structure of the small molecule dye is shown as a formula I or a formula II. The nano photosensitizer OBDlp modified by outer membrane vesicles of genetically engineered bacteria serves as a novel nano photosensitizer, tumor targeting PTT is mediated through NIR II FL / PA imaging, tumor immunogenicity is improved, antigen presenting cells are activated, and infiltration and functionalization of CD8 + T cells are caused. And OBDlp and laser are combined to induce release of a large amount of DAMPs and trigger ICD to promote maturation of cDC1s. Meanwhile, a CD47 nano antibody carried by the nano photosensitizer induces polarization of M1 phenotype macrophages by blocking a CD47-SIRP alpha signal transduction axis, and an immunosuppression tumor microenvironment is reversed.

The invention relates to the technical field of cosmetics, and particularly discloses a composition with after-sun repairing and whitening effects and a preparation method thereof. According to the present invention, various efficient components such as the ergothioneine compound, the acetylchitosamine, the nicotinamide, the sodium hyaluronate, the inositol, the aloe vera extract, the glycerophosphoinositide choline salt, the tetrahydromethylpyrimidine carboxylic acid, the oat beta-glucan compound, the citrus peel extract compound and the skin brightening agent are scientifically matched; according to the present invention, the excellent after-sun repairing and whitening effect is achieved, the comprehensive after-sun skin care is achieved, the skin state can be improved, and the skin health degree can be improved. The application creatively finds that when the glycerophosphoinositide choline salt, the citrus peel extract compound and the skin brightening agent are compounded, the effects of the composition in the aspects of after-sun repairing and whitening can be improved at the same time, and the comprehensive effect is relatively good.

25HC3S choline and crystalline 25HC3S choline are described herein. Pharmaceutical formulations of 25HC3S choline such as with crystalline 25HC3S choline and methods of treating or preventing disease with same such as hypercholesterolemia, hypertriglyceridemia, and conditions related to fat accumulation and inflammation (e.g., non-alcoholic fatty liver disease (NAFLD), non-alcoholic steatohepatitis (NASH), alcoholic hepatitis, acute kidney injury (AKI), psoriasis, and atherosclerosis) are further disclosed herein. Methods for preparing 25HC3S, including crystalline 25HC3S choline, are also provided.

Provided herein are pharmaceutical compositions and formulations of a conjugate comprising a first protein, such as an anti-VEGF antibody, conjugated to a phosphorylcholine-containing polymer, and a second protein that is not conjugated to a phosphorylcholine-containing polymer. The second protein can be the same as the first protein, or can be different. In some embodiments, the pH of the formulation is different from the isoelectric point (pI) of the second protein so as to prevent or reduce turbidity of the formulation. Also provided are methods of preparing the formulations, methods of use thereof, and kits with pre-filled syringes containing the formulations.

The present invention discloses a preparation method of yellow azoxy and azo stilbene dyes. Using 4-nitrotoluene-2-sulfonic acid as a raw material and water as a reaction solvent, in the presence of choline hydroxide, dye yellow 11 is obtained through a condensation reaction. The molecular structure of choline hydroxide contains a quaternary ammonium salt group and a hydroxyl group. The hydroxyl group provides additional hydrophilicity, which helps to improve the solubility and dispersibility of the dye in an aqueous medium and enhance the affinity between the dye and the fiber. Choline hydroxide can effectively provide the alkaline environment required for the reaction, promote the synthesis reaction of the dye, and better control the selectivity and yield of the reaction. Since the choline hydroxide molecule contains a hydroxyl group, it can form hydrogen bonds with the sulfonic acid groups in the dye molecule, enhance the stability between dye molecules, and improve the light fastness and acid and alkali resistance of the dye. Using choline hydroxide not only optimizes the reaction conditions, improves the purity and yield of the dye, but also significantly improves the performance of dye yellow 11.

The invention provides universal functional formula milk powder for cats and dogs and a preparation method thereof, and relates to the field of pet formula food. The formula milk powder consists of goat milk powder, colostrum powder, whey protein powder, lactoferrin powder, yolk powder, immune globulin powder, compound grease, compound minerals, compound vitamins, choline, taurine, compound prebiotics, compound probiotics, docosahexaenoic acid, arachidonic acid, casein phosphopeptides, L-glutamine and beta-galactosidase. And the goat milk powder, colostrum powder, whey protein powder, lactoferrin powder, yolk powder and immune globulin powder are subjected to low-temperature sterilization treatment by adopting an induced electric field or high-pressure microjet. The cat and dog formula milk powder overcomes the defects in the prior art, through compounding of basic nutritional ingredients and functional ingredients, a novel low-temperature sterilization technology is adopted for sterilization treatment, on the premise that the functionality of the formula milk powder is guaranteed, the nutritional value of the formula milk powder is reserved to a greater extent, and healthy growth and development of cats and dogs are promoted.

The Lactobacillus plantarum VB165 strain, which was collected in the China General Microbiological Culture Collection Center on 28 September 2022 under preservation number CGMCC NO. 25839. When simulating in intestinal environment, the strain can inhibit the activity of α-glucosidase, can degrade bile salts, inhibits the activity of primary pathogenic gut bacteria, and possesses good tolerance to gastric acid and choline; said strain can be applied in the preparation of products to control blood glucose.

The invention discloses a brassica rapa imago feed and a preparation method thereof, and belongs to the technical field of insect breeding. The Eye spot Brassica rapa adult feed comprises 11 to 15 parts of casein, 1 to 3 parts of Webster salt, 4 to 6 parts of cane sugar, 9 to 12 parts of cellulose powder, 0.2 to 0.4 part of L-cysteine, 0.2 to 0.3 part of choline chloride, 0.08 to 0.1 part of inositol, 0.325 to 0.4 part of composite vitamin, 2 to 3 parts of a nutritional ingredient ethanol solution, 12 to 14 parts of a nutritional ingredient aqueous solution, 1 to 2 parts of honey, 2 to 3 parts of agar powder, 0.03 to 0.04 part of cholesterol, 33 to 67 parts of apple jam, 0.3 to 4 parts of royal jelly and 80 to 120 parts of water. In addition, the invention also provides a preparation method of the feed for the Eye spot Brassica rapa imagoes. The feed for the Eye spot Brassica rapa adults can prolong the life of the adults and improve the egg laying amount at the same time.

The invention provides an inhalable tanshinone IIA lipid nanocrystal as well as a preparation method and application thereof, and the inhalable tanshinone IIA lipid nanocrystal is mainly formed by entrapping tanshinone IIA by using dipalmitoyl phosphatidylcholine DPPC and dipalmitoyl phosphatidyl ethanolamine DPPE as mixed phospholipids in the presence of a stabilizer. Compared with the prior art, the inhalable tanshinone IIA lipid nanocrystal disclosed by the invention has the advantages that by improving the physicochemical property and drug release behavior of TA, the lung retention volume of TA after aerosol inhalation is obviously increased, the retention time of TA in the lung is prolonged, and the risk of toxic and side effects caused by whole-body escape of drugs is reduced; the treatment effect on idiopathic pulmonary fibrosis is improved, and the compliance of a patient is improved.

The invention belongs to the field of bioengineering, and particularly relates to phosphorylcholine cytidyltransferase and application thereof in preparation of citicoline, and the specific application is that the phosphorylcholine cytidyltransferase can efficiently catalyze phosphorylcholine and CTP to react to generate citicoline. The phosphorylcholine cytidyltransferase is prepared by the following steps: synthesizing a coding gene of the phosphorylcholine cytidyltransferase, selecting pHT01 as an expression vector, co-expressing with choline kinase, transforming into host bacteria, and inducing protein expression. According to the choline phosphate cytidyltransferase disclosed by the invention, under the same reaction condition and within the same reaction time, the yield of a citicoline product obtained by conversion is higher than that of choline phosphate cytidyltransferase (ScCCT) which is most commonly used in the market at present and is derived from saccharomyces cerevisiae S288c. Specifically, the yield of the MsCCT001 is 10 times higher than that of the ScCCT, the yield of the MbCCT006 is 6 times higher than that of the ScCCT, and the yield of the MeCCT008 is 8 times higher than that of the ScCCT.

The invention belongs to the technical field of biology, and particularly relates to an SLC44A1 gene and / or SLC44A5 gene knockout animal model as well as a construction method and application thereof. The construction method of the animal model comprises the following steps: deleting exon regions from the fourth exon region to the thirteenth exon region of the SLC44A1 gene and / or the second exon region of the SLC44A5 gene in mouse brain oligodendroglia cells in a high-efficiency manner by utilizing gene editing and a Cre-loxP recombinase system, so as to realize the knockout of a target gene. The animal model constructed by the invention can be used for researching the regulation mechanism of the choline transporters (SLC44A1 and SLC44A5) on myelin sheath formation, deeply knowing the biological functions of the choline transporters (SLC44A1 and SLC44A5), simulating the occurrence and development processes of human related diseases, revealing the pathogenesis of the related diseases, providing a new thought for prevention and treatment of brain myelin sheath dysplasia, and providing a new foundation for the prevention and treatment of brain myelin sheath dysplasia. And an effective drug or a treatment mode aiming at the disease is screened by utilizing a gene knockout animal model, so that a new choice is provided for clinical treatment of the disease.

The invention relates to the technical field of bioengineering, and discloses a genetically engineered bacterium and a method for producing icaritin through whole-cell catalysis. By constructing double plasmids, choline kinase, isopentenyl phosphokinase, flavonoid 3-hydroxylase, flavonol synthase, isopentenyl transferase and oxymethyltransferase are simultaneously overexpressed in escherichia coli, so that a genetically engineered bacterium is constructed, and the engineering bacterium is used as a whole-cell catalyst to prepare a whole-cell engineering bacterium. The naringenin and the 3-methyl-2-butene-1-ol are used as substrates to be converted into the icaritin in a whole-cell catalysis mode, only one-step reaction is needed, the method is simple, rapid and high in efficiency, other chemical substances do not need to be additionally added in the conversion process, the cost is reduced, pollution to the environment is relieved, and the method is suitable for industrial production. And the biological production from low-price naringenin to high-added-value icaritin is realized.

The invention provides a method for preparing high-purity phosphatidylserine by a batch enzyme catalysis method based on dynamic choline removal, and belongs to the technical field of enzymatic reaction. According to the method disclosed by the invention, in a reaction system for preparing phosphatidylserine, a byproduct choline is selectively adsorbed by adopting resin. Choline in a reaction system is removed in real time through the integrated adsorption separation module, the enzyme inhibition problem is solved, and meanwhile, the PC conversion rate is larger than or equal to 98% and the PS purity is larger than or equal to 96% in combination with the immobilized enzyme circulation technology; the enzyme can be reused for more than or equal to 20 times (activity retention is more than or equal to 90%); and the choline residual concentration is less than or equal to 0.5 mM (an inhibition threshold value or less).

The invention provides a medical hydrophilic coating and a preparation method and application thereof.The coating comprises a substrate layer and a functional layer, the substrate layer is arranged on the surface of a medical base material, the functional layer is arranged on the surface of the side, away from the medical base material, of the substrate layer, and the substrate layer comprises a substrate modification material containing a first reactive functional group; the functional layer comprises a phosphorylcholine compound containing a second reactive functional group; the substrate layer and the functional layer are connected through a chemical bond formed by the first reactive functional group and the second reactive functional group; the substrate modification material contains a third reactive functional group, and the substrate layer is connected with the medical base material through a chemical bond formed by the third reactive functional group and hydroxyl or amino on the surface of the medical base material; when the first reactive functional group is an azide group, the second reactive functional group is an alkynyl group; or when the first reactive functional group is the alkynyl group, the second reactive functional group is the azide group. Chemical bonds are formed through azido and alkynyl, multiple chemical reactions are not needed, and the uniformity of the coating is improved.

The invention relates to the technical field of bacterium enrichment nutrient solutions, in particular to a bacterium enrichment nutrient solution for fermentation and a preparation method of the bacterium enrichment nutrient solution. The culture medium is prepared from the following raw materials in parts by weight: maltodextrin, crystalline glucose, low-pigment yeast extract powder, refined soy peptone, dipotassium phosphate, monopotassium phosphate, magnesium sulfate heptahydrate, sodium chloride, phytic acid, L-ascorbic acid, manganese sulfate monohydrate, biotin, thiamine hydrochloride and choline chloride. Through a double-carbon-source structure, maltodextrin and crystalline glucose cooperate, metabolic oscillation is avoided, and the energy utilization rate is increased; the low-pigment yeast extract powder and the refined soy peptone are complementary, so that the rapid growth requirement of thalli is met; phytic acid is chelated with metal ions to prevent precipitation, L-ascorbic acid is used for resisting oxidation, and biotin and thiamine activate a coenzyme pathway to realize accurate regulation and control of a metabolic network.

The invention provides a lactobacillus plantarum VB165, which is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on September 28, 2022, and the preservation number is CGMCC NO.25839. The lactobacillus plantarum VB165 is named as VB165. The lactobacillus plantarum VB165 has the advantages that the lactobacillus plantarum VB165 is named as VB165; under the condition of simulating the intestinal environment, the strain can inhibit the activity of alpha-glucosidase, also has the activity of degrading cholate and inhibiting main intestinal pathogens, has good tolerance to gastric acid and choline, and can be applied to preparation of blood sugar control products.

The invention provides a choline chloride: urea deep eutectic solvent modified ZIF-8 high-temperature proton exchange membrane as well as a preparation method and application thereof, and belongs to the technical field of preparation of fuel cells and proton exchange membranes. The deep eutectic solvent and the zinc hydroxide nanowire membrane are compounded in situ through a suction filtration method, and then the deep eutectic solvent modified ZIF-8 high-temperature proton exchange membrane is prepared. In the ZIF-8 high-temperature proton exchange membrane, a zinc hydroxide nanowire membrane bears a choline chloride-urea deep eutectic solvent, so that the choline chloride-urea deep eutectic solvent is compounded into pores of ZIF-8 in situ, and the choline chloride-urea deep eutectic solvent is blocked in a ZIF-8 membrane to modify the inner wall; the preparation method is simple to operate, low in energy consumption and free of pollution, and the prepared ZIF-8 high-temperature proton exchange membrane can maintain stability, has extremely high proton conduction capacity and has very good practicability.

The invention belongs to the technical field of agricultural biology, and discloses a method for inducing curcuma wenyujin tissue culture seedling test-tube micro rootstocks, which comprises the following steps: inoculating terminal bud cluster proliferation buds of curcuma wenyujin obtained by conventional tissue culture into a rootstock induction culture medium for rooting culture for 30 days to obtain tissue culture seedlings with rootstocks, the rhizome induction culture medium is prepared from 1 / 2MS, 0.1-1.0 mg / L of NAA, 30 g / L of cane sugar, 1-3 mg / L of choline chloride and 1-3 mg / L of compound sodium nitrophenolate, the pH is 5.8, and the weight ratio of choline chloride to compound sodium nitrophenolate is 1: (0.3-2). According to the method, the formation of the curcuma wenyujin test-tube micro rhizomes is induced in vitro, the sterile micro rhizomes are directly transplanted, and the micro rhizome artificial seeds are manufactured for sowing, so that a green seedling hardening and temporary planting link is omitted, and the seedling loss caused by strict requirements of green seedlings on humidity is reduced, and the test-tube micro rhizome seedlings are beneficial to storage and long-distance transportation; therefore, the seedling breeding efficiency of the rhizome plants is improved.

The present disclosure provides unshielded lipid nanoparticles and a process that enables the production of such unshielded lipid nanoparticles, thereby overcoming previous challenges of making particles without causing aggregation thereof. The lipid nanoparticles comprise a nucleic acid cargo molecule; a sterol or a derivative thereof present at a content of at least 12 mol%; a neutral lipid, such as a phospholipid having a choline head group present at a content of between 22 mol% and 65 mol%; and an ionizable cationic amino lipid present at a content of between 15 mol% and 45 mol%; wherein the lipid nanoparticle is non-sterically stabilized with a hydrophilic polymer-lipid conjugate, or otherwise unshielded and wherein each mol% content is relative to total lipid present in the lipid nanoparticle.

The invention provides a lignin extraction-sulfonic acid modification integrated preparation method based on eutecticevaporate solvent coupling microwaves, lignin sulfonate and application, and belongs to the technical field of oilfield chemistry. The preparation method comprises the following steps: mixing choline chloride, epichlorohydrin and 4-hydroxybenzenesulfonic acid, heating and stirring to obtain a deep eutectic solvent ternary system; the method comprises the following steps: adding biomass powder into a deep eutectic solvent ternary system, and reacting under a microwave condition; after the reaction is completed, filtering, and adjusting the pH value of the obtained filtrate to be neutral to obtain a mixed solution; and then adding an organic solvent into the obtained mixed solution for precipitation, filtering, washing and drying to complete lignin extraction-sulfonic acid modification. According to the method, in-situ grafting of sulfonic acid functional groups is realized in the lignin separation process through the ternary eutectic solvent in combination with microwave-assisted extraction, so that sulfonation modification of lignin is completed. After sulfonation modification, the obtained sulfonated lignin is applied to the drilling fluid, and the viscosity reduction effect can be achieved.

The invention provides a lactobacillus gasseri strain A21120 with anti-oxidation, anti-inflammatory and anti-tumor functions as well as a probiotic agent and application of the lactobacillus gasseri strain A21120, and belongs to the technical field of functional microorganisms. The strain A21120 is separated and screened from intestinal feces of healthy old people of 100 years old, and belongs to lactobacillus gasseri through morphological identification and molecular identification. The strain has good gastrointestinal fluid resistance and bovine choline resistance and has the potential of colonizing intestinal tracts. The lactobacillus gasseri strain A21120 provided by the invention has bacteriostatic activity, anti-inflammatory activity, anti-oxidation activity and anti-tumor activity, and provides a new way for treatment of clinical related diseases, drug development and preparation of in-vitro preparations or daily necessities.

The present disclosure provides unshielded lipid nanoparticles and a process that enables the production of such unshielded lipid nanoparticles, thereby overcoming previous challenges of making particles without causing aggregation thereof. The lipid nanoparticles comprise a nucleic acid cargo molecule; a sterol or a derivative thereof present at a content of at least 12 mol %; a neutral lipid, such as a phospholipid having a choline head group present at a content of between 22 mol % and 65 mol %; and an ionizable cationic amino lipid present at a content of between 15 mol % and 45 mol %; wherein the lipid nanoparticle is non-sterically stabilized with a hydrophilic polymer-lipid conjugate, or otherwise unshielded and wherein each mol % content is relative to total lipid present in the lipid nanoparticle.

The invention discloses a method for extracting enteromorpha polysaccharide by using an ultrasonic-assisted deep-eutectic solvent, which comprises the following steps: rinsing silt on the surface of fresh enteromorpha with clear water, drying, freeze-drying, crushing, filling enteromorpha powder into a sealed bag, and refrigerating in a refrigerator for later use; the preparation method comprises the following steps: by taking 1, 2-propylene glycol as a hydrogen bond donor and choline chloride as a hydrogen bond receptor, adding deionized water to prepare a deep-eutectic solvent; the method comprises the following steps: weighing enteromorpha powder, mixing with a deep eutectic solvent, carrying out water bath and ultrasonic-assisted extraction, and centrifuging to obtain an enteromorpha polysaccharide leaching solution; and adding absolute ethyl alcohol into the enteromorpha polysaccharide extract, uniformly mixing, standing at 4 DEG C for 12 hours, centrifuging, taking precipitate, redissolving, carrying out rotary evaporation on the precipitate, deproteinizing by adopting a Sevage method, dialyzing, decoloring, taking supernate, and freeze-drying, so as to obtain the enteromorpha polysaccharide. The method disclosed by the invention has the advantages of high efficiency, short time consumption, high yield, simplicity in operation and the like, is mild in extraction condition, safe, green and environment-friendly, and lays a foundation for extraction of enteromorpha polysaccharide in food industry.

The metabolic marker can be used for early diagnosis of heart failure, has the advantages of high sensitivity, rapidness, convenience and accurate and reliable result, provides a basis for clinical decision, provides a certain basis for subsequent fundamental research and clinical research, and has potential application and research values. The metabolic marker comprises one or more of 1-(4-nitrophenyl) piperidine, sphingolipid d19: 3 / 23: 0, phosphatidyl ethanolamine 16: 1e / 22: 5, phosphatidyl ethanolamine 16: 1e / 22: 6, sphingolipid d14: 0 / 22: 1, 3-phosphoglyceric acid, phosphatidylcholine 15: 0 / 20: 4, phosphatidylcholine o-16: 1 / 18: 0, phosphatidylcholine 14: 0e / 20: 1, phosphatidylcholine 17: 0 / 18: 5, betaine, phosphatidylcholine 15: 0 / 18: 2 and phosphatidylcholine 18: 5e / 20: 4.