22 results about "Cyanocobalamin" patented technology

The invention relates to the technical field of chemiluminescence immunoassay, in particular to a simple and efficient vitamin B12 detection kit and a detection method.The simple and efficient vitamin B12 detection kit comprises a pretreatment agent, a magnetic particle system and a marker system, an extracted serum sample is mixed with the protein releasing agent and the complexing agent in the pretreatment agent, and the vitamin B12 in the sample is released from the binding protein; the complexing agent is a complex with cyanide ions, the cyanide ions separated from the complex in an aqueous solution are combined with the released vitamin B12 to obtain stable cyanocobalamin for subsequent detection of the vitamin B12, and the vitamin 12 in a to-be-detected sample is converted into a stable state for detection by the method, so that the detection stability is improved.

The invention relates to a method for producing mecobalamin by using a micro-channel reactor, which comprises the following steps: firstly, introducing a reaction mixed solution of cyanocobalamin, cobalt chloride, sodium borohydride and purified water into a first micro-channel reactor for reduction reaction; the obtained cyanocobalamin solution in the reduction state is introduced into a second microchannel reactor to be subjected to a methylation reaction with a trimethylsulfoxide iodide solution, and finally, an obtained mecobalamin solution is subjected to post-treatment to obtain a finished product. Compared with the prior art, the micro-channel reactor is used for continuous production, the mass and heat transfer effect is good, the reaction temperature can be accurately controlled, side reactions are further reduced, and the product quality is effectively improved.

The application provides a method for detecting the titer of vitamin B12 in a fermentation liquor. The method comprises the following steps: preparing a potassium dichromate solution, a hydrochloric acid solution and a potassium dihydrogen phosphate solution, mixing the three solutions with the vitamin B12 fermentation liquor under certain conditions to generate cyanocobalamin, and then testing the titer of the cyanocobalamin by using a high performance liquid chromatography. The titer of the vitamin B12 detected by using the method is consistent with the titer detected by using a sodium cyanide method. The operation method greatly reduces the safety risk of an analyst caused by using the sodium cyanide, and the operation method is simple, easy to manage in a laboratory and can save the testing cost.

The present invention provides an ophthalmic composition that can directly reach the extraocular muscles and treat or prevent their fatigue. [Solution] An ophthalmic composition containing one or more compounds selected from the group consisting of azulene sulfonic acid, cyanocobalamin, and salts thereof, wherein the compounds reach the extraocular muscles. An ophthalmic composition containing one or more compounds selected from the group consisting of azulene sulfonic acid, cyanocobalamin, and salts thereof, used for the treatment and / or prevention of fatigue of the extraocular muscles.

The present invention relates to a nutritional vitamin complex for pets intended to improve the fur and skin quality of pets such as dogs and cats. The composition of the complex contains omega-3, curcumin, piperine, biotin (vitamin H or vitamin B7), vitamin B2 (riboflavin), vitamin B3 (nicotinic acid or niacin), vitamin B5 (pantothenic acid), vitamin B6 (pyridoxine), vitamin B12 (cyanocobalamin), folic acid (vitamin B9), vitamin E (alpha-tocopherol), vitamin C, zinc (zinc glycine chelate), coenzyme Q10, hydrolysed type II collagen, astaxanthin, DL-methionine, selenium, L-glycine, L-proline, L-phenylalanine, L-leucine, L-isoleucine, L-histidine, L-arginine, L-lysine, L-tyrosine, resveratrol, and a flavouring agent.

The present invention relates to a nutritional vitamin complex for pets intended to improve the fur and skin quality of pets such as dogs and cats. The composition of the complex contains omega-3, curcumin, piperine, biotin (vitamin H or vitamin B7), vitamin B2 (riboflavin), vitamin B3 (nicotinic acid or niacin), vitamin B5 (pantothenic acid), vitamin B6 (pyridoxine), vitamin B12 (cyanocobalamin), folic acid (vitamin B9), vitamin E (alpha-tocopherol), vitamin C, zinc (zinc glycine chelate), coenzyme Q10, hydrolysed type II collagen, astaxanthin, DL-methionine, selenium, L-glycine, L-proline, L-phenylalanine, L-leucine, L-isoleucine, L-histidine, L-arginine, L-lysine, L-tyrosine, resveratrol, and a flavouring agent.

The present disclosure describe a simple optimized method that allows the combined manufacture of five active substances: cyanocobalamin, pyridoxine, thiamine, pregabalin and celecoxib in a single solid, stable, immediate release pharmaceutical form for pain. The combined use of drugs that act at distinct levels is associated with greater pain relief and, as they can be used at lower doses, with a reduced risk of adverse effects. In this context, the combined use of B vitamins plus pregabalin and celecoxib would be associated with greater analgesic activity through complementary action mechanisms.

This invention provides a method for producing adenosylcobalamin. First, cyanocobalamin, cobalt chloride, adenine, nucleoside triphosphate, and water are mixed and added to a reaction vessel to prepare an adenosylcobalamin mixture. High-purity nitrogen and a NaBH4-NaOH aqueous solution are added during the reaction. Then, the adenosylcobalamin filtrate is purified by passing it through a purification column. The purified solution is then subjected to adsorption and elution on a resin column, and the eluent is crystallized to obtain pure adenosylcobalamin. This purification process effectively removes impurities introduced during the synthesis of adenosylcobalamin, with total impurities ≤0.5%, ensuring the safety and controllability of product quality.

This invention discloses a strain of *Lactobacillus paracasei* capable of converting cyanocobalamin and promoting its absorption, thereby enhancing brain nerve repair and improving cognition, and its postbiotic, belonging to the fields of microbial technology and pharmaceutical technology. The *Lactobacillus paracasei* CCFM1527 and its prepared postbiotic provided by this invention promote the growth of vitamin B1 in the host. 12 The neuroprotective effects of upregulating the expression of absorption receptors and converting CN-CbI to methylcobalamin include: (1) promoting the upregulation of Amnionless expression in intestinal cells (Caco-2); (2) converting CN-CbI to methylcobalamin; and (3) promoting the upregulation of Amnionless expression in mouse ileum. AMN Upregulation of gene expression (4) significantly increased the levels of vitamin B in mice. 12 (5) Significantly promotes the expression of MBP and PSD-95 proteins in mice; (6) Improves the decline in cognitive ability in mice, and has great application prospects.

The invention is applicable to the technical field of feeds, and provides a special nutrient enrichment premix for boars and a preparation method thereof. The compound feed additive is prepared by compounding beta-carotene, 25-hydroxyvitamin D3, dl-alpha-tocopheryl acetate, menadione sodium hydrogen sulfite, ammonium sulfate nitrate, riboflavin, pyridoxine hydrochloride, cyanocobalamin, nicotinamide, D-calcium pantothenate, folic acid, D-biotin, L-ascorbic acid, DL-methionine, zinc methionine chelate, selenium yeast, chromium picolinate, L-carnitine and glucose according to a specific proportion. A dynamic batching and high-precision mixing process is adopted for preparation. Through the synergistic effect of various nutrients, the sow milk quality can be remarkably improved, the birth weight of piglets is increased by 50 g or above, the survival rate is increased by 6-8% or above, the weaning weight is increased by 500 g or above, meanwhile, the sow estrus rate is increased by 5% or above, the problems that sows are poor in body condition, low in reproductive performance, low in piglet survival rate and the like in existing boar breeding are effectively solved, and extremely high breeding application value is achieved.

To provide cosmetics that are aesthetically superior by suppressing discoloration over time and the precipitation of ingredients contained in the cosmetics. [Solution] By including (A) hydrolyzed gardenia extract, (B) cyanocobalamin, and (C) cyclohexane-1,4-dicarboxylic acid bisethoxydiglycol, the cosmetic composition can suppress discoloration over time and precipitation of components contained in the cosmetic composition, resulting in superior aesthetics. The cosmetic composition may also contain (A) hydrolyzed gardenia extract in an amount of 0.0005 to 0.05% by mass, (B) cyanocobalamin in an amount of 0.0005 to 0.005% by mass, and (C) cyclohexane-1,4-dicarboxylic acid bisethoxydiglycol in an amount of 0.05 to 0.5% by mass.

The disclosed invention relates to a chemical composition that can be taken orally and supplements an unhealthy diet and lifestyle. In one aspect of the invention the chemical composition includes Retinol Palmitate, Thiamine, Riboflavin, Niacin, Nicotinamide mononucleotide (NMN), Pyridoxal, Folic Acid, Cyanocobalamin, Cholecalciferol, Zinc, Manganese, Copper, Calcium, Magnesium, Iron, L-Selenomethionine, Lysine, Phenylalanine, L-Threonine, L-Methionine, L-Histidine, L-Tryptophan, L-Leucine, L-Isoleucine, L-Valine, Cinnamon Bark Powder, and Curcumin.

The present invention relates to a nutritional composition comprising one or more micronutrients, wherein the one or more micronutrients comprise vitamin A, pyridoxine (vitamin B6), folic acid (vitamin B9), cyanocobalamin (vitamin B12), ascorbic acid (vitamin C), cholecalciferol (vitamin D3), vitamin E, calcium, and / or selenium. Also provided are corresponding kits, methods of manufacturing and using the same, and methods of determining a subject's nutritional requirements.

A composition of the present invention is used for oral administration. The composition incudes thiamine at least 5% by weight, pyridoxine at least 5 wt. %; cyanocobalamin at least 0.1 wt. %, polysorbate 40 at least 1.8 wt. %, and water for the rest, up to 100 wt. %. The composition provides high concentrations of pyridoxine, thiamine, cyanocobalamin, stability for a long period of storage under standard conditions.

The present disclosure relates to a composition for enhancing skin elasticity or alleviating skin wrinkles. The composition for enhancing skin elasticity or alleviating skin wrinkles of the present disclosure exhibits a remarkable synergistic effect on enhancing skin elasticity or alleviating skin wrinkles by means of a combination of adenosine triphosphate and cyanocobalamin.

The invention relates to the technical field of coffee drinks, and discloses a refreshing coffee drink containing vitamin B. The preparation method comprises the following steps: S1, stirring and mixing vitamin B1 hydrochloride, vitamin B6 pyridoxine hydrochloride, vitamin B12 cyanocobalamin and beta-cyclodextrin, and carrying out ultrasonic treatment in a water bath for 20-25 minutes to form microcapsule particles; s2, stirring and mixing Arabic gum, vegetable protein and coffee components, adding ascorbyl palmitate, and performing spray drying at 60-65 DEG C to obtain particles A; s3, adding the microcapsule particles, caramel color and nicotinamide into deionized water, stirring at 75-80 DEG C, adding the particles A, mixing, and adding Arabic gum to obtain a mixed emulsion; and S4, carrying out ultra-high temperature instantaneous sterilization (UHT, 130-135 DEG C, sterilization), cooling to room temperature, and carrying out sterile filling. The coffee disclosed by the invention has a relatively good refreshing effect.

The invention relates to the technical field of food nutrition, and particularly discloses a composition for strengthening physique and improving resistance, which is prepared from the following raw materials by weight: 40 to 100 g of L-ascorbic acid, 0.5 to 2 g of cyanocobalamin, 0.2 to 1.2 g of thiamine hydrochloride, 0.2 to 1.2 g of riboflavin, 0.1 to 0.8 g of pyridoxine hydrochloride, 10 to 30 g of zinc lactate, 0.5 to 2 g of 2% selenized carrageenan, 15500 to 21500 g of milk powder, 600 to 1400 g of lactose and 5 to 15 g of stevioside. Especially, the plant extract, the amino acid and the antioxidant are further added for combination, so that the immunity of the composition can be remarkably improved; the formula is designed according to the principles of modern nutriology and traditional herbal medicine, can provide various vitamins, minerals, amino acids and herbal extracts required by the human body in daily life, and is helpful for enhancing the defense mechanism of the body and improving the reaction of the immune system.

The present invention provides a skin cosmetic containing cyanocobalamin that provides a moisturizing effect while also improving skin elasticity, caring for pores, and enhancing skin texture and radiance. [Solution] The skin cosmetic comprises (A) cyanocobalamin, (B) sugar alcohol, and (C) nonionic surfactant. Furthermore, the skin cosmetic may have at least one component (B) selected from inositol, maltitol, and sorbitol, and component (C) may have at least one component selected from polyoxyethylene sorbitan fatty acid ester and polyoxyethylene hydrogenated castor oil.

The invention belongs to novel medical application of known medicines, and particularly relates to application of cyanocobalamine in preparation of a Brucella bacteriostatic agent. In the specific embodiment of the invention, firstly, four main chemical forms (cyanocobalamin, hydroxycobalamin, methyl cobalamin and adenosine cobalamin) of the vitamin B12 are added into a Brucella culture medium to culture Brucella, and experimental results show that the four forms of vitamin B12 can obviously inhibit the growth of Brucella; wherein the inhibition effect of the cyanocobalamine is most obvious. Then, cyanocobalamin with different concentrations is added into a Brucella culture medium to culture Brucella, and the experimental result shows that the CFU of the Brucella is obviously reduced along with the increase of the concentration of the cyanocobalamin. Similarly, cyanocobalamine can also significantly inhibit intracellular proliferation of Brucella at the cellular level. The cyanocobalamine is a clinically common medicine, and no side effect is reported at present, so that the cyanocobalamine can be used for preparing the Brucella bacteriostatic agent and is clinically used for preventing and treating Brucella diseases.

The present invention relates to a medium composition for culturing Faecalibacterium prausnitzii composed of vegetable peptone, yeast extract, a phosphate compound, a carbonate compound, cyanocobalamin (B12), L-cysteine, ammonium acetate, and maltose, and a method of mass-culturing Faecalibacterium prausnitzii using the medium composition. When the medium composition and the method for culturing Faecalibacterium prausnitzii according to the present invention are used, high-density culture and mass production of strains of Faecalibacterium prausnitzii phylogroup II are possible. In addition, when Faecalibacterium prausnitzii is mass-cultured in the medium composition free of animal components, it may be cultured at high density even in high-concentration anaerobic nitrogen gas, and thus it is possible to provide a culture method which is more economical and suitable for industrialization.

The present invention relates to a simple optimized method that allows the combined manufacture of five active substances: cyanocobalamin, pyridoxine, thiamine, pregabalin and celecoxib in a single solid, stable, immediate release pharmaceutical form for pain. The combined use of drugs that act at distinct levels is associated with greater pain relief and, as they can be used at lower doses, with a reduced risk of adverse effects. In this context, the combined use of B vitamins plus pregabalin and celecoxib would be associated with greater analgesic activity through complementary action mechanisms.

This invention discloses a bergamot effervescent tablet based on microencapsulation technology and its preparation method, relating to the field of solid beverage technology. The invention comprises, based on a total feed weight of 100 kg, the following components: bergamot extract; 3 kg; anhydrous citric acid; 18 kg; sodium bicarbonate; 15 kg; dextrin; 45 kg; gum arabic; 0.6 kg; tragacanth gum; 0.15 kg; taurine; 1.58 kg; zinc gluconate; 0.75 kg; zinc citrate; 0.25 kg; calcium gluconate; 0.25 kg; ascorbic acid C; 0.1 kg; ferrous gluconate; 0.11 kg; thiamine hydrochloride; 0.058 kg; riboflavin; 0.03 kg; pyridoxine hydrochloride; 0.02 kg; cyanocobalamin; 0.001 kg; folic acid; 0.001 kg; isomaltitol; 15 kg; sucralose; 0.1 kg. The advantages are: through systematic screening of key factors such as the inclusion of aromatic oil components in bergamot extract, the ratio of acid-base disintegrants, the ratio of flavoring agents and food fortifiers, the type of fillers, the preparation method of acid-base particles, and the tableting process parameters.