111 results about "Cyanocobalamin" patented technology

A dietary supplement of mitochondrial nutrients is designed for relieving stress, preventing and improving stress-related disorders, such as chronic fatigue syndrome, diabetes, age-associated cognitive dysfunction and diseases (Parkinson's and Alzheimer's disease). The supplement composition has the following nutrients: B vitamins (cyanocobalamin 2-1,000 ug, thiamin 1-1,000 mg, niacin 15-2,000 mg, pyridoxine 1-1,000 mg, Pantothenate 5-150 mg, folic acid 400-40,000 ug), alpha-tocopherol 10-800 mg, ascorbic acid 50-10,000 mg, calcium 20-2,000 mg, vitamin A 200-10,000 ug, alpha-lipoic acid 100-1,000 mg, N-acetyl cysteine 100-3,000 mg, L-carnosine 100-9,000 mg, tyrosine 100-9,000 mg, vanillin 10-100 mg, phosphatidylserine 10-800 mg, resveratrol 10-50 mg, dehydroepiandrosterone 1-50 mg, and melatonin 0.1-3 mg, all of which have been individually used experimentally or clinically for relieving stress, preventing and treating age- and stress-related disorders and diseases but no combination of these compounds has been used. Many embodiments also contain at least one adjunct ingredient such as coenzyme Q 10-200 mg, acetyl-L-carnitine 100-2,000 mg, choline 50-1,000 mg, and creatine 100-2,000 mg.

A method for administering an antioxidant composition to humans according to their age and sex is disclosed wherein the method comprises administering to said humans a daily dose of a multiple antioxidant micronutrient composition comprising vitamin A (palmitate), beta carotene (from natural d. salina), vitamin C (calcium ascorbate), vitamin D-3 (cholecalciferol), natural source vitamin E including both d-alpha tocopheryl and d-alpha tocopheryl acid succinate, thiamine mononitrate, riboflavin, niacinamide ascorbate, d-calcium pantothenate, pyridoxine hydrochloride, cyanocobalamin, folic acid (folacin), d-biotin, selenium (1-seleno methionine), chromium picolinate, zinc glycinate, calcium citrate, and magnesium citrate. For persons over the age of about 51, the composition preferably further comprises one or more of co-enzyme Q10, N-acetyl cysteine, and alpha lipoic acid. Preferably, also, vitamin D is added for women over the age of about 36.

The invention relates to a liposome lyophilized preparation prepared from twelve compound vitamins. The lyophilized preparation is characterized in that the lyophilized preparation is prepared from a liposome which consists of soybean lecithin and glycocholic acid and is encapsulated with twelve vitamins of retinyl palmitate, cocarboxylase tetrahydrate, riboflavin sodium phosphate, pyridoxine hydrochloride, cyanocobalamin, cholecalciferol, ascorbic acid, racemization-alpha tocopherol, D-biotin, niacinamide, folacin and dexpanthenol.

A stable pharmaceutical mercury-free aqueous solution of cyanocobalamin comprised of cyanocobalamin and water wherein said solution of cyanocobalamin is suitable for intranasal administration, has a viscosity less than about 1000 cPs, and wherein said solution of cyanocobalamin has a bioavailability of cyanocobalamin when administered intranasally of at least about 7% relative to an intramuscular injection of cyanocobalamin with the proviso that the solution is essentially free of mercury and mercury-containing compounds. The present invention is also directed towards a method for elevating the vitamin B12 levels in the cerebral spinal fluid (CSF) comprising administering intranasally a sufficient amount of a mercury-free cyanocobalamin solution so as to increase the average ratio of vitamin B12 in the CSF to that in the blood serum (B12 CSF / B12 Serum×100) to at least about 1.1 comprising intranasally administering an aqueous solution of a cyanocobalamin, wherein said solution of cyanocobalamin has a bioavailability of at least 7% relative to an intramuscular injection of a cyanocobalamin.

A new electrochemical method for determining dissolved oxygen concentration is developed. It employs cobalt based oxide or complex to modify a conductive electrode surface. This method can be applied to determine dissolved oxygen in regard to medical and environmental demands. The chemical formula of the cobalt based oxide or complexes can be shown as CoxOy or CoaLb. Both “x” and “a” represent the number of cobalt atom; while “y” and “b” represent the number of oxygen atom and the number of coordinating ligand, respectively. For instance, the best known example of cobalt oxide is CO3O4, and the best known examples of cobalt complexes are cobalt phthalocyanine (CoPC) and cyanocobalamin (vitamin B12), or other macrocyclic complexes with a metallic nucleus of cobalt. The present invention determines the concentration of dissolved oxygen in solution in potentiostatic mode with three electrodes. As a result, the dissolved oxygen can be determined between 0.0V and −0.3V vs. Ag / AgCl, thereby significantly reducing the interferences from the easily oxidized compounds in water solutions.

The present disclosure concerns methods and compositions to inhibit insects from biting a subject. In preferred embodiments, the compositions may be administered orally, for example using a spray bottle to deliver to the mouth. In certain embodiments, the compositions and methods are effective to reduce swelling, itching, redness and / or inflammation of the local area of an insect bite. The compositions may include one or more herbs selected from the group consisting of rice bran, peppermint, barley grass, lobelia; chlorella, watercress, alfalfa and parsley and one or more vitamins selected from the group consisting of thiamin (B-1), riboflavin (B-2), niacin (B-3), pantothenic acid (B-5), pyridoxine (B-6), folic acid (B-9), cyanocobalamin (B-12), choline, inositol, d-biotin, para-aminobenzoic acid, and lecithin. Administration of effective amounts of the compositions is sufficient to inhibit insects from biting and / or treat insect affected areas of a subject.

The present invention relates generally to the use of vitamin B12 (cobalamin or cyanocobalamin) alone or in combination with other photoprotective agents, including specifically other vitamins such as vitamin B9 (folic acid or folate) and vitamin B3 (niacin or niacinamide), or any chemical derivative of these vitamins and their salts, as a filter to protect cells against the damaging effects of ultraviolet (UV) light. The invention is, in one aspect, a method of reducing the rate of UV damage to cells exposed to a UV light source, by treating the cells with the vitamin composition, either alone or in combination with other photoprotective agents. Other aspects of the invention are compositions comprising effective amounts of vitamin B12 alone or in combination with other photoprotective agents including vitamin B9 and vitamin B3 and a pharmaceutically-acceptable carrier, that are useful in protecting cells, particularly skin cells, against the burning, genotoxic (mutagenic and carcinogenic), immunosuppressive and photoaging effects of UV light, especially sunlight. The invention has application as a UV light filter in oral preparations including tablets and drinks, topical creams, lotions, sprays, wipes and cosmetics. The invention also has application as a medicinal treatment for dermatological conditions caused by exposure to sunlight, such as actinic keratoses, photodermatitis, photo-induced (discoid) lupus erythematosus and the photosensitizing effects of a variety of drugs used commonly in clinical practice (e.g. certain antihistamines, ACE inhibitors, and antibiotics such as tetracycline).

A supplement composition for enhancement of methylation process is provided, which contains vitamin B6 (as pyridoxine HCl), folic acid, vitamin B12 (as cyanocobalamin), betaine HCl, and methylsulfonylmethane; and also contains S-adenosylmethionine. The supplement composition further includes silymarin (from milk thistle seed extract), N-acetyl L-cysteine, and cruciferious blend which includes broccoli (brassica oleracea var. talica), kale (brassica oleracea var. acephala), and radish (raphanus sativus). Further provided is a method of using the supplement composition for enhancement of methylation process.

A method for optimizing the health of humans according to their age and sex is disclosed wherein the method comprises administering to said humans a daily dose of a multiple antioxidant micronutrient composition comprising vitamin A (palmitate), beta-carotene (from natural d. salina), vitamin C (calcium ascorbate), vitamin D-3 (cholecalciferol), natural source vitamin E including both d-alpha tocopherol and d-alpha tocopheryl acid succinate, thiamine mononitrate, riboflavin, niacinamide ascorbate, d-calcium pantothenate, pyridoxine hydrochloride, cyanocobalamin, folic acid (folacin), d-biotin, selenium (l-seleno methionine), chromium picolinate, zinc glycinate, calcium citrate, and magnesium citrate. For persons over the age of about 51, the composition preferably further comprises one or more of co-enzyme Q10, N-acetyl cysteine, and alpha lipoic acid. Preferably, also, vitamin D is added for women over the age of about 36.

The present invention relates to a metal complex of the general formula M(L)n, wherein each L is independently selected and represents a ligand and at least one L is vitamin B12 (cyanocobalamin) or a derivative thereof bound through the nitrogen atom of its cyanide group to M, which is an element selected from the transition metals, thus, forming a M-NC-[Co] moiety with [Co] representing vitamin B12 without cyanide and wherein n is 1, 2, 3, 4, 5 or 6. The complex can be prepared by mixing a precursor molecule with vitamin B12. The metal complexes can be used for radiodiagnostics, chemotherapy and radionuclide therapy.

The invention relates to an oral multi-vitamin nano liposome, belonging to the technical field of nutrient supplements. In multi-vitamin nano liposome oral liquid, nano liposome prepared from lecithin, cholesterol and tween-80 is used as an embedding agent, and the liposome contains vitamin A1, vitamin D3, vitamin E, vitamin K, vitamin C, vitamin B1 (thiamine), vitamin B2 (riboflavin), vitamin B3 (nicotinic acid), vitamin B5 (pantothenic acid), vitamin B6 (pyridoxine), vitamin B7 (biotin), vitamin B9 (folic acid) and vitamin B12 (cyanocobalamin). The oral multi-vitamin nano liposome can be directly absorbed and utilized by small intestines, so that the absorption rate and the bioavailability of the oral multi-vitamin nano liposome are improved; and the oral multi-vitamin nano liposome can be widely applied to the industries of health-care food, functional beverage, cosmetics, medicine and the like, and is suitable for large-scale production.

The invention discloses a novel chemical synthesis method for preparing mecobalamine. The novel chemical synthesis method comprises the steps of: dissolving cyanocobalamin and cobalt chloride hexahydrate in deionized water to obtain reaction liquid, simultaneously dropwise adding a sodium borohydride aqueous solution and a methanol / formaldehyde solution to the reaction liquid, and generating mecobalamine in one step through reductive methylation. According to the novel chemical synthesis method, the cyanocobalamin is taken as a raw material, and the mecobalamine is synthesized in one step; and therefore, the novel chemical synthesis method is simple in operation and has low requirements on equipment, little pollution and wide application prospect, and conditions are easy to control.

The present invention discloses a vitamin B12 preparation method, VB12 fermentation broth is directly heated for hydrolysis to release intracellular VB12, cyanocobalamin salt can be obtained by secondary membrane filtration, drum centrifuging protein removal, protein adsorption, and other impurity removal processes, and nanofiltration concentration dehydration, and sodium cyanide convertion, and pure product cyanocobalamin, namely VB12 can be obtained by three-level resin adsorption column impurity removal and crystallization separation. According to the method, filtration-aid-free proteins accounting to more than 80% of the total protein can be obtained, protein and mycelium can be used for further processing to produce high value-added products, the dosage of sodium cyanide can be reduced by more than 90% compared that of a traditional process, production cost is reduced, due to high removal rate of proteins and other impurities in macromolecular solutions, no poisonous cyanide-containing mycelium is produced, the cation exchange resin adsorption step is eliminated, one- time investment is reduced, production time is shortened, productivity is improved, and VB12 total yield reaches 83%.

The invention provides a pharmaceutical composition of 12 complex vitamins for injection and a preparation method thereof. The pharmaceutical composition of 12 complex vitamins for injection provided by the invention comprises the active ingredients of vitamin A palmitate, cholecalciferol, racemic alpha-tocopherol, ascorbic acid, nicotinamide, dexpanthenol, pyridoxine hydrochloride, riboflavin sodium phosphate, tetrahydrate thiamine pyrophosphate, folic acid, D-biotin and cyanocobalamin, and auxiliary materials namely polysorbate 80 and mannitol. The prescription provided by the invention does not contain auxiliary material glycocholic acid, and can be clinically used for people with over-high glycocholic acid.

The invention relates to the field of skin care products, in particular to a skin activating and whitening essence liquid and a preparation method thereof. The skin activating and whitening essence liquid comprises 1,3-propylene glycol, butylene glycol, nicotinamide, panthenol, hydrolyzed collagen, trehalose, tocopherol, glycerin, 3-o-ethyl ascorbic acid and cyanocobalamin. The nicotinamide, the 3-o-ethyl ascorbic acid, the panthenol, the cyanocobalamin, the tocopherol and multivitamins synergistically cooperate with the butanediol, the hydrolyzed collagen, the trehalose and other raw materials to prepare the skin activating and whitening essence liquid, and the skin activating and whitening essence liquid can improve skin metabolism, promote the absorption of skin care raw materials, effectively improve the self-protection of the skin, and improve skin dullness, declined elasticity, pigmentation and other poor skin conditions; vitamin B12 and vitamin C complement with each other to improve the skin metabolism, promote the absorption of nutrient raw materials, effectively improve the self-protection of the skin and improve skin dullness, declined elasticity and other poor skin conditions. Long term use of the skin activating and whitening essence liquid can dilute spots, make the skin color uniform, whiten and brighten the skin, clear the skin, remove skin imperfections and make the skin shining, permeable, bright and white.

The invention discloses an anti-stress regulator used for promoting growth and improving metabolism of pigs. The regulator is prepared from the following ingredients by weight: retinyl acetate, vitamin D3, d1-alpha-tocopheryl acetate, menadione, thiamine hydrochloride, riboflavin, pyridoxine hydrochloride, cyanocobalamin, L-ascorbic acid, d-biotin, nicotinamide, pantothenic acid, folic acid, methionine, lysine, xylooligosaccharide, citric acid, sodium glutamate and glucose as balance. The invention also relates to a preparation method of the anti-stress regulator used for promoting growth and improving metabolism of pigs. The regulator prepared by the method can stimulate the self-metabolism regulation, can improve the feed utilization rate, can improve the food consumption of the growing-finishing pigs and the reproductive performance of the sows, can improve the anti-stress ability, can reduce the death rate and the diarrhea occurrence rate of piglets, has a remarkable growth promotion effect, can improve the growth speed of the weaned pigs, the growing-finishing pigs and the suckling pigs, and has remarkable economic benefit.

A sports drink mixture for making a sports drink that remains palatable while providing nutritional and energy benefits without causing negative physiological responses in the human body includes compositions of: tapioca starch, further including compositions of unprocessed and pregelatinized tapioca starch; bromelain; natural flavors; citric acid; vitamin E oil; stevia; potassium phosphate; sodium citrate; magnesium chloride; ascorbic acid; niacinamide; d-calcium pantothenate; pyridoxine hydrochloride; riboflavin; thiamine hydrochloride; folic acid; biotin; and cyanocobalamin. A sports drink includes compositions of the sports drink mixture with an aqueous liquid.

A new electrochemical method for determining dissolved oxygen concentration is developed. It employs cobalt based oxide or complex to modify a conductive electrode surface. This method can be applied to determine dissolved oxygen in regard to medical and environmental demands. The chemical formula of the cobalt based oxide or complexes can be shown as CoxOy or CoaLb. Both “x” and “a” represent the number of cobalt atom; while “y” and “b” represent the number of oxygen atom and the number of coordinating ligand, respectively. For instance, the best known example of cobalt oxide is Co3O4, and the best known examples of cobalt complexes are cobalt phthalocyanine (CoPC) and cyanocobalamin (vitamin B12), or other macrocyclic complexes with a metallic nucleus of cobalt. The present invention determines the concentration of dissolved oxygen in solution in potentiostatic mode with three electrodes. As a result, the dissolved oxygen can be determined between 0.0V and −0.3V vs. Ag / AgCl, thereby significantly reducing the interferences from the easily oxidized compounds in water solutions.

Provided is a kit comprising a compound having vitamin B-12 activity in a form that is suitable for injection. The kit can be in the form of a box with a lid with a number of components placed in the box. The compound having vitamin B-12 activity can be cyanocobalamin, typically in form of a solution in a vial. The kit can contain an alcohol pad. The kit can contain a pair of gloves. The kit can contain a csr wrap. The concentration of the cyanocobalamin can be 1000 mcg / ml. In one embodiment, the kit is a box with a lid and following components placed inside of the box: a) a container having 1 milliliter (mL) of a solution of cyanocobalamin with a concentration of 1000 mcg / ml; b) isopropyl pad; c) a pair of gloves; and d) a csr wrap.

The invention discloses a whitening microemulsion containing multiple vitamins. Synergistic action of multivitamins such as 3-o-ethyl ascorbic acid is used, tocopherol, nicotinamide, panthenol and cyanocobalamin to scavenge free radicals and block melanin release. Accelerate melanin metabolism to achieve deep whitening and effectively improve melanin deposition. At the same time, the present invention incorporates a microemulsion for microemulsification of vitamins, which makes the vitamins more easily absorbed, thereby better acting on the skin to whiten and freckle.

The invention relates to the field of skin care products, in particular to a mineral spring rejuvenating lotion and a preparation method thereof. The mineral spring rejuvenating lotion comprises water, 1,3-propylene glycol, glycerinum, trehalose, nicotinamide, cyanocobalamin, phenoxyethanol, allantoin, sodium hyaluronate, 3-o-ethyl ascorbic acid, panthenol and hydrolyzed collagen. Through a synergistic effect of multiple vitamins of the nicotinamide, 3-o-ethyl ascorbic acid, panthenol, cyanocobalamin and tocopherol, in cooperation with raw materials such as hydrolyzed collagen, trehalose and sodium hyaluronate, the mineral spring rejuvenating lotion is prepared, skin pores are purified, convenience is provided for the skin to absorb nutrient components, the water flocculation texture of the skin is restored, the translucent feeling and softness of the cuticle are maintained, and a mineral spring skin care lotion is supplemented, so that the skin feels active, moisturized and transparent; the vitamin B12 and sodium hyaluronate supplement each other, the skin metabolism is improved, absorption is promoted, the self-protection force of the substrate is effectively improved, and the conditions of dull skin and lowered elasticity are reduced; the spots are faded, the skin color is balanced, whiting and shining are achieved, the skin is transparent and free of speckles, and the skinis lustrous, transparent, bright and white.

The invention provides a diabetes full-nutrition formula food and a preparation method thereof. The formula food comprises maltodextrin, resistant dextrin, xylitol, fruit and vegetable powder, gingerpowder, soy isolate protein, whey protein, vegetable fat powder, inulin, fructo-oligosaccharide, konjaku flour, probiotic powder, retinyl acetate, vitamin D3 powder, D-alpha-tocopheryl acetate powder,vitamin K1, thiamine hydrochloride, riboflavin, pyridoxine hydrochloride, cyanocobalamin, nicotinamide, folic acid, D-calcium pantothenate, L-sodium ascorbate, D-biotin, copper sulfate, magnesium sulfate, ferrous sulfate, zinc citrate, manganese sulfate, calcium hydrophosphate, potassium iodide, sodium selenite and potassium chloride. The diabetes full-nutrition formula food is rich in nutrition,can improve the microenvironment in the body of a patient, can resist oxidative stress of hyperglycemia on the body, and reduces body injury. The preparation method of the formula food has the advantages that the process is simple and easy to control and the formula food is suitable for wide popularization.

The invention relates to a detection method of vitamin B12 and a sample pretreatment method of vitamin B12, and belongs to the technical field of detection of biological samples. The pretreatment method comprises the following steps of obtaining a to-be-detected sample solution, and adding a protein releasing agent and a complexing agent, wherein the protein releasing agent is used for releasing the vitamin B12 of a to-be-detected sample from binding protein; the complexing agent is a complex with cyanide ions, and the total stability constant beta of the complex in a water solution is 1010 to 1045; dissociating the cyanide ions from the complex in the solution, binding the cyanide ions and the released vitamin B12, and forming stable cyanocobalamine for measuring. The detection method and sample pretreatment method have the advantages that the hypertoxic cyanide is replaced with the low-toxic complex with the cyanide ions, the dissociated trace cyanide ions can react with the vitamin B12 in the to-be-detected sample, and the stable cyanocobalamine is produced; the requirement of sample treatment is met, the sample can be fully treated, and the larger toxicity is not produced.

The invention belongs to the technical field of medicine, and particularly relates to a target composition capable of improving light cognition impairment and a preparation method thereof. The targetcomposition is prepared from the following components in percentage by weight: 20 to 30 percent of L-carnitine, 15 to 30 percent of fructus alpiniae oxyphyllae extract, 10 to 30 percent of folium ginkgo extract, 10 to 30 percent of boxthorn leaf extract, 0.005 to 0.02 percent of cyanocobalamin, 0.01 to 0.03 percent of L-5-methyl-tetrahydrofolic acid and 10 to 30 percent of auxiliary material. Thetarget composition has a remarkable effect when used for improving light cognitive impairment.

The invention discloses nutritional supplement food containing isolated soybean protein. The isolated soybean protein and milk powder are taken as raw materials, soybean milk powder, non-dairy creamer, glucose and edible salt are taken as auxiliary materials, vitamin A, vitamin D3, tocopheryl acetate, thiamine hydrochloride, riboflavin, pyridoxine hydrochloride, cyanocobalamin, L-sodium ascorbate, folic acid, ethylenediaminetetraacetic acid ferric sodium salt, zinc gluconate and tricalcium phosphate are added and taken as nutritional ingredients, and the nutritional supplement food containing the isolated soybean protein is prepared. The functional food can meet the demands of a human body for intake of balanced nutritional ingredients, comprehensive, appropriate and targeted supplementation of nutritional substances required by the human body is realized, and the nutritional supplement food is appropriate functional food which has complete nutrition and is particularly suitable for children, pregnant women, lactating women and the elderly with weaker body functions.

A radioactive protection micronutrient formulation system is provided and the system comprises: a formulation consisting essentially of antioxidants, the antioxidants are selected from the group consisting essentially of vitamin C, vitamin E, N-acetyl cysteine, natural mixed carotenoids, and alpha-lipoic acid, vitamin A (palmitate), vitamin D-3 (cholecalciferol), thiamine mononitrate, riboflavin, niacinamide ascorbate, d-calcium pantothenate, pyridoxine hydrochloride, cyanocobalamin, folic acid, D-Biotin, selenium (1-seleno-methionine), chromium picolinate, zinc glycinate, calcium citrate and magnesium citrate and mixtures thereof; and plus a booster formulation selected from a group consisting essentially of vitamin C, d-alpha tocopheryl acid succinate, alpha tocopherol, N-acetyl cysteine, natural mixed carotenoids and alpha lipoic acid, the formulation is designed to reduce the risk in humans exposed to doses of ionizing radiation of becoming subjected to at least one condition selected from the group consisting essentially of radiation-induced acute leukemia, breast cancer, thyroid cancer and other somatic and heritable mutations.

The invention relates to an age-related complementary food nutrition bag rich in hypoallergenic heterologous immunocompetence peptides and a preparation method thereof, and belongs to the technical field of age-related nutrition supplement food. Twelve nutrient supplement factors are formed on three low-sensitization protein-based carriers, namely sea cucumber protein peptide powder, terrestrial plant source-soybean protein peptide powder and terrestrial animal source high-quality protein-skimmed milk powder for the first time; I-level steady-state protection of nutrient supplementation factors, II-level steady-state protection formed on a low-sensitization protein-based carrier and a III-level steady-state protection technology of double quantitative precise filling are designed, and the senile complementary food nutrition bag rich in the low-sensitization heterologous immunocompetence peptide is created; and the symbolic characteristics of low sensitization, high immunocompetence and high stability of the compound are confirmed through inspection of three indexes including a BALB / C mouse sensitization evaluation model, a mouse macrophage RAW264.7 cell model and the decay rate of vitamin A acetate, cholecalciferol and cyanocobalamin, and technical support is provided for development of series products.

The invention is applicable to the technical field of cosmetics, and provides an anti-blue-light and anti-oxidation composition, a preparation method and application thereof. The composition compriseslycopene liquid, glucosylrutin, a nutritional supplement and a complexing agent, wherein the nutritional supplement comprises at least one of serine, threonine, tryptophan, tyrosine, valine, vitaminH, folic acid, cyanocobalamin, vitamin B3, pantothenic acid, vitamin B6, vitamin B2, vitamin B1 and yeast polypeptides; the complexing agent comprises EDTA-2Na and an emulsifying agent. The composition has strong blue light filtering capability, can block harm of blue light to skin, effectively removes free radicals generated by blue light irradiation, reduces skin damage caused by excessive freeradicals, and can play roles in inhibiting inflammation, supplementing skin nutrients and brightening skin.

Mesophilic methane bacteria obtained from digested sludge are acclimatized in an H2 / CO2 medium and the acclimatized methane bacteria are grown on a support inclusive of inorganic nutritional salts of trace metal elements by using an immobilized bed bioreactor. The grown bacteria are allowed to metabolize a mixed gas, prepared by adding hydrogen to at least one of coal gas and biogas, into methane and at the same time, cobalamin contained in the fermented liquor is recovered as an extracellular product in the form of cyanocobalamin by using potassium cyanide to thereby produce vitamin B12 efficiently in a high content and high yield.