1324 results about "Nicotinamide" patented technology

The invention relates to the technical field of biomarkers, in particular to a blood biomarker combination for prediction and auxiliary diagnosis of ovarian reserve hypofunction (DOR), a corresponding detection kit and application of the blood biomarker combination. According to the invention, the content change of the nicotinamide mononucleotide (NMN) and the metabolite combination thereof in serum or plasma is screened for the first time and can be used as a detection index for predicting the ovarian reserve function decline. Based on the biomarker combination, the invention further provides an in-vitro detection kit containing a related standard substance, an internal standard solution and an extracting solution, and a data analysis method combined with a random forest model, so that an individual with a normal ovarian reserve function and an individual with hypofunction can be accurately distinguished. The method is noninvasive, simple, convenient, high in sensitivity and suitable for early recognition and layered screening of clinical ovarian reserve hypofunction.

The invention relates to the technical field of cosmetics, in particular to an acne-removing composition, lipidosome and a preparation method and application thereof. The acne-removing composition disclosed by the invention is prepared from the following components in parts by weight: 2 to 8 parts of nicotinamide, 1 to 5 parts of cortex magnoliae officinalis extract, 0.15 to 2.01 parts of glycyrrhizic acid, 0.1 to 2 parts of totarol and 0.01 to 0.15 part of hyssopus officinalis extract. The nano-carrier is adopted to wrap and deliver the acne removal composition, so that the solubility of the functional components is increased, the bioavailability of the functional components is improved, meanwhile, the irritation of the functional components is also improved, multi-target co-delivery and multi-mechanism acne removal are realized, and the acne removal composition has a remarkable acne removal effect.

Provided are a whitening and freckle-removing composition, a gel, a preparation method therefor, and a use thereof, relating to the technical field of skin-care products. The whitening and freckle-removing composition comprises tranexamic acid, nicotinamide, 4-butylresorcinol, licorice extract, 3-o-ethyl ascorbic acid, and glutathione. For the whitening composition, by inhibiting the activity of tyrosinase, blocking the transfer of melanin from melanocytes to keratinocytes, and inhibiting the formation and accumulation of melanin by means of multiple mechanisms including oxidation resistance, and by means of the synergistic action of all components, cosmetic benefits of whitening and freckle-removing are achieved. The whitening and freckle-removing gel has a simple preparation method, has a mild and non-sensitizing formula, is safe and non-irritating, and is suitable for large-scale production and application.

Provided herein is a process for preparing compound A comprising (a) admixing 2-isopropyl-4-methylpyridin-3-amine (Compound B), or a salt thereof, a first base, and a reactive compound comprising phosgene or a phosgene equivalent in an organic solvent to form 3-isocyanato-2-isopropyl-4-methylpyridine (Compound C); (b) admixing Compound C and 2,6-dichloro-5-fluoronicotinamide (Compound D) to form 2,6-dichloro-5-fluoro-N-((2-isopropyl-4-methylpyridin-3-yl)carbamoyl)nicotinamide (Compound E); and (c) admixing Compound E and a second base to form a product mixture comprising Compound A and the second base. Also provided herein is a process for synthesizing AMG 510 comprising using Compound A prepared according to the disclosed processes

The invention discloses an anti-aging face cream suitable for sensitive skin and a preparation method of the anti-aging face cream. The anti-aging face cream comprises 1-10 wt% of a supramolecular eutectic compound, 10-30 wt% of an auxiliary material and the balance of deionized water, the supramolecular eutectic compound comprises an anti-aging component, a supramolecular eutectic agent and Neosol-Aqulio, wherein the supramolecular eutectic agent is used for forming a supramolecular eutectic with the anti-aging component, and the Neosol-Aqulio is dispersed in the supramolecular eutectic; wherein the anti-aging component comprises oleuropein and hydroxytyrosol, and the supramolecular eutectic agent is nicotinamide, or a mixture of capryloyl glycine and betaine. Neosol-Aqulio, a supramolecular eutectic agent and an anti-aging component are utilized to form a supramolecular eutectic, so that active component delivery is facilitated, efficient transdermal absorption is realized, permeability, stability and mildness of the anti-aging active component are improved, the problem of sensitive skin cuticle permeation is solved, irritation of a traditional penetration enhancer is avoided, and the penetration enhancer is suitable for clinical application. The double effects of efficient permeation and mild anti-aging are realized.

The invention relates to the technical field of whitening essence, and discloses whitening essence suitable for sensitive skin and a preparation method thereof.The whitening essence suitable for sensitive skin is prepared from, by mass, 2%-3% of prinsepia utilis royle oil, 1%-2% of portulaca oleracea extract, 1.5% of camellia yunnanensis flower extract, 0.5% of panax notoginseng root extract, 3% or less of nicotinamide, 1% or less of 1, 2-propylene glycol and the balance deionized water. 1%-1.5% of 1, 2-hexanediol, 0.1%-0.3% of sodium hyaluronate and 60%-70% of deionized water. According to the whitening essence suitable for the sensitive skin and the preparation method of the whitening essence, through the synergistic combination of prinsepia utilis royle oil (barrier repair), purslane (anti-inflammatory), camellia japonica (tyrosinase inhibition) and pseudo-ginseng (microcirculation improvement), melanogenesis is inhibited through multiple channels, the sensitive skin is relieved synchronously, nicotinamide (3%) is compounded with the prinsepia utilis royle oil, and the skin whitening effect is achieved. The synergism of whitening and barrier repair is enhanced through a dual mechanism of promoting ceramide synthesis and inhibiting melanin transport, and the problems that a traditional whitening agent has different degrees of irritation, allergy phenomena such as skin redness, swelling and pain are easily caused by improper use, and a skin intolerance phenomenon exists are solved.

The invention relates to the technical field of cosmetics, in particular to moisturizing and anti-aging nanoemulsion, a preparation method thereof and moisturizing essence. The moisturizing and anti-aging nanoemulsion comprises the following raw materials in percentage by mass: 90-99% of an anti-aging component and 1-10% of a moisturizing component, and the anti-aging component comprises the following raw materials in percentage by mass: 1-20% of a glycosylglycerol solution, 0.5-3% of nicotinamide adenine dinucleotide, 0.1-5% of sodium coramate and the balance of a solvent; the moisturizing component comprises ceramide lipidosome. The raw materials in the moisturizing and anti-aging nanoemulsion synergistically exert effects, the moisturizing and anti-aging effects of the moisturizing and anti-aging nanoemulsion are improved, meanwhile, the stability and applicability of the moisturizing and anti-aging nanoemulsion are enhanced, and the moisturizing and anti-aging nanoemulsion is mild and non-irritant to skin and safe to use. The preparation method of the moisturizing anti-aging nanoemulsion is simple in process, high in production efficiency and beneficial to industrial production. The moisturizing essence has more remarkable moisturizing and anti-aging effects by adding the moisturizing and anti-aging nanoemulsion, and is safe to use.

The invention provides anti-aging moisturizing essence and a preparation method thereof, and belongs to the technical field of cosmetics. The essence is prepared from a plant extract, sodium pyrrolidone carboxylate, hexapeptide, nicotinamide mononucleotide, vitamin B5, galactose yeast fermentation product filtrate, b-glucan, sodium hyaluronate, glycerol, tranexamic acid, blue copper peptide, arbutin, nicotinamide, vitrin, a bifidus yeast fermentation product, a bifidus yeast fermentation lysate and the like. Wherein the plant extract is prepared by mixing rosemary, frankincense resin, tea flowers, sandalwood and jasmine flowers through ultrasonic extraction, comprehensive skin care is achieved from multiple angles of anti-aging, whitening, moisturizing and repairing, the overall health of the skin is guaranteed, and the skin care cream is suitable for various skin types.

The invention belongs to the technical field of stem cell biological preparations, and particularly relates to an anti-aging compound preparation based on stem cells and application of the anti-aging compound preparation in delaying skin aging. The composition is composed of an inner water phase, a middle oil phase and an outer water phase, wherein the inner water phase is prepared from the following components: stem cell exosomes, hyaluronic acid, nicotinamide ribose, trehalose, mannitol, glycine and a citric acid buffer solution; the medium oil phase is prepared from the following components: medium chain triglyceride, shea butter, vitamin E succinate, PEG (Polyethylene Glycol) modified ceramide NP and cholesterol succinic acid monoester; the external water phase is prepared from the following components: polydatin, cellulose nanocrystals and an emulsifying agent. On the basis of a W / O / W emulsion system, a multi-layer anti-aging mechanism is constructed by utilizing a three-dimensional delivery framework of an inner water phase, an oil phase and an outer water phase and space-time synergy of active ingredients, the exosome permeation efficiency is improved through microneedle introduction, and the anti-oxidation and barrier repair effects are achieved by using multiple ingredients.

The invention relates to the technical field of daily chemicals, and particularly discloses a scalp oil control composition for preventing alopecia seborrhoeica as well as a preparation method and application of the scalp oil control composition. The scalp oil control composition is prepared from the following main components: nicotinamide, capryloyl glycine, piralinone ethanolamine salt, betaine salicylic acid, lemon peel extract, white willow bark extract and rice fermentation filtrate. All the components have a synergistic effect, and the effects of controlling oil, protecting hair and preventing alopecia seborrhoeica are achieved. The preparation method disclosed by the invention is simple and convenient, and the obtained product is high in bioavailability of active ingredients, good in hair care effect and low in irritation, has the effects of caring hair, strengthening hair roots, regulating scalp micro-ecology and preventing seborrheic alopecia, and can be widely applied to related washing and care products for preventing seborrheic alopecia.

The invention discloses a xanthophyll-nicotinamide eutectic crystal and a preparation method and application thereof, the eutectic crystal is formed by xanthophyll and nicotinamide according to the molar ratio of 1: 1 through intermolecular interaction of hydrogen bonds, Van der Waals force and the like, and the molecular formula is C46H62N2O3. According to the invention, xanthophyll and nicotinamide are combined through a co-crystal technology, a novel co-crystal with good water solubility and high bioavailability is formed, and the solubility and absorption efficiency of xanthophyll are significantly improved.

The invention relates to the related technical field of daily chemical skin care products, in particular to a water aqua solution for scalp care and a preparation method thereof.The water aqua solution is prepared by mixing multiple raw materials in sequence, and the multiple raw materials comprise, by concentration, 0.005% of mixed cytokines, 0.005% of auxiliary agents and the balance water. The addition amount of the vitamin B12 is 0.00006%; the addition amount of allantoin is 0.2%; the addition amount of nicotinamide is 0.7%; the adding amount of the chamomile extracting solution is 5%; the adding amount of the aloe extracting solution is 5%; the addition amount of the ginkgo biloba extract is 5%; the addition amount of the lucid ganoderma extracting solution is 5%; the addition amount of the polyol preservative is 1%; the total percentage is 100%; according to the application, the mixed cell factors, the vitamin B12, the allantoin, the nicotinamide, the chamomile extracting solution, the aloe, the gingko, the lucid ganoderma, the polyol preservative and the water are mixed, so that the scalp absorption efficiency is improved, the safety of the product can be improved, and the maintenance effect is more obvious; the problems that an existing aqueous solution for skin curing is low in component action efficiency and insufficient in adaptability and safety are solved.

The invention relates to a nicotinamide ribose phosphate transferase mutant, a genetically engineered bacterium and application of the nicotinamide ribose phosphate transferase mutant and the genetically engineered bacterium. Compared with a wild enzyme strain NMN, the production capacity of a mutant enzyme strain is greatly improved. The saccharomyces cerevisiae genetically engineered bacterium capable of stably inheriting and efficiently synthesizing the NMN is obtained, and safe, efficient and green synthesis of the NMN is achieved.

The invention provides a nicotinamide nucleotide transhydrogenase mutant and application thereof, valine at the 169th site of the nicotinamide nucleotide transhydrogenase mutant is designed to be mutated into isoleucine, the isoleucine is used for constructing a lysine production strain, and the new strain does not contain plasmids, does not have growth defects, does not need induction, and has the advantages of good genetic stability, high fermentation yield and the like. The lysine can be efficiently and stably produced from the beginning by taking glucose as a substrate.

A nutraceutical composition, consisting of Nicotinamide Mononucleotide in a range 250-1000 mg, Spermidine in a range 0.5-10 mg, Rhodiola Rosea in a range 200-600 mg, Cacao in a range 1000-10,000 mg, L-Theanine in a range 50-200 mg, Glucosamine in a range 500-3000 mg, and Hyaluronic acid in a range 50-240 mg, or consisting of Epigallocatechin-3-gallate in a range 100-500 mg, Quercetin in a range 250-1000 mg, Resveratrol in a range 150-1000 mg, Sulforaphane in a range 1-50 mg, and Calcium Alphaketoglutarate in a range 300-2000 mg, or consisting of Microdose Lithium in a range 1-5 mg, Trimethylglycine in a range 500-2000 mg, Glutathione in a range 250-1000 mg, Fisetin in a range 100-1000 mg, Urolithin A in a range 250-2000 mg, Sodium Butyrate in a range 100-3000 mg, Berberine in a range 500-1500 mg, Apigenin in a range 50-500 mg, Cinnamon in a range 1000-6000 mg, and Malate in a range 1000-6000 mg.

The invention discloses an essence composition containing lipidosome with an acne removing effect as well as a preparation method and application of the essence composition. The essence composition comprises the following components: 2-30% of lipidosome with the acne removing effect, 0.5-15% of a skin conditioner, 5-40% of a humectant, 0.1-3% of a preservative, 0.15-10% of a thickening agent, 0.05-5% of a chelating agent, 0.01-3% of a pH regulator and deionized water. The lipidosome with the acne removing effect is prepared from the following components in percentage by weight: 1.5 to 2.5 percent of white willow bark extract, 0.3 to 0.7 percent of Pinosine, 2 to 4 percent of rhizoma bletillae root extract, 4 to 6 percent of nicotinamide, 0.15 to 0.35 percent of oligopeptide-3, 7 to 12 percent of lecithin and 1 to 2 percent of cholesterol. In the preparation process, the lipidosome with the acne removal effect is reasonably compounded with other auxiliary material matrixes, so that efficient transmission and absorption of active ingredients can be promoted, irritation to skin can be effectively relieved, meanwhile, the overall effect of the essence is enhanced, the essence has mildness and high efficiency, and the high-efficiency requirement of consumers for the acne removal skin care product is met.

The present invention relates to the technical field of daily chemical technology, and specifically provides a whitening and freckle-removing cream. The freckle-removing cream comprises the following components in parts by weight: water, glycerin, mineral oil, antioxidant microcapsules, polydimethylsiloxane, sodium ascorbyl phosphate, niacinamide, a plant compound extract, essence, and cetearyl alcohol. The present invention adds natural plant extract ingredients, which is safe and effective. A circulation device is used to promote the effective combination of active ingredients, so that the active ingredients can quickly penetrate into the inner layer of the skin, thereby improving the transdermal absorption ability of the active ingredients. A unique encapsulation technology is used to firmly adhere the active ingredients to the skin surface, thereby effectively lightening facial spots, removing dullness from the skin, and restoring luster.

The present invention relates to the technical field of cosmetics, and discloses a whitening and freckle-removing essence and a preparation method thereof. The whitening and freckle-removing essence is formed by mixing additives such as nicotinamide, EDTA, L-lactic acid, citric acid, baicalein inclusion complex, etc. The baicalein inclusion complex has a coated structure. Since the skin surface carries a negative charge and the coating layer of the baicalein inclusion complex contains chitosan, it can be adsorbed on the skin surface through electrostatic action, producing a sustained-release effect, and then targeted release, so as to improve the whitening effect of the essence. In addition, after being encapsulated, baicalein can have good compatibility with other substances in the formula. Moreover, the linolenic fatty acid chain in the structure of the chitosan derivative has excellent antioxidant, anti-inflammatory and other effects, which can reduce the phenomenon of scar formation on the skin due to inflammation.

The invention provides a nicotinamide phosphoribosyltransferase mutant and an application of the nicotinamide phosphoribosyltransferase mutant in preparation of beta-nicotinamide mononucleotide. According to the nicotinamide phosphoribosyltransferase mutant, 259th lysine (K) of an amino acid sequence shown in SEQ ID NO: 2 is mutated into 259th valine (V). The invention overcomes the technical problems of slow reaction, low yield, high energy consumption and NMN feedback inhibition of the product in the process of producing NMN by escherichia coli, and the constructed nicotinamide phosphoribosyltransferase mutant expression system is used for fermentation production of nicotinamide mononucleotide, and slows down the feedback inhibition effect.

The invention discloses a beautifying essence which comprises the following components in percentage by weight: 1% of bifidus yeast fermentation liquor, 3% of boine, 2% of nicotinamide, 0.3% of gallnut extract, 1% of purslane extract, 0.3% of allantoin, 0.2% of centella asiatica extract, 0.3% of tocopherol extract, 0.5% of aesculus chinensis extract, 2% of vitamin E, 0.05% of xanthan gum, 0.05% of sodium hyaluronate, 0.2% of carbomer, 0.5% of p-hydroxyacetophenone, 0.5% of 1, 2-propylene glycol and the balance of water. The cleaning agent is prepared from, by weight, 0.5% of 1, 2-hexanediol, 5% of glycerin, 4% of PEG / PPG-14 / 7 dimethyl ether, 2.2% of glycosylglycerol, 0.2% of tetrahydroxypropyl ethylenediamine, 3% of polyglycerol-3, 0.05% of PDRN powder and the balance water. Effect evaluation test results show that the beauty essence can inhibit the activity of elastase and increase the content of type I collagen in human primary fibroblasts, so that the firming and anti-wrinkle effects are achieved.

The invention relates to the field of skincare products, and particularly discloses skincare gel loaded with ganoderma lucidum exosome and a preparation method of the skincare gel. The skin care gel loaded with the lucid ganoderma exosome is prepared from the following raw materials in parts by weight: 20 to 40 parts of hydrogel matrix, 2 to 4 parts of lucid ganoderma exosome nanoparticles, 2 to 6 parts of niobium carbide nanosheets, 1 to 3 parts of nicotinamide, 0.1 to 2 parts of phenethyl resorcinol and 0.1 to 3 parts of green tea extract, the Mailing exosome nanoparticles are of a core-shell structure, a core layer comprises Mailing exosome and papain in a mass ratio of 1: (0.1-0.2), and a shell layer comprises a polylactic acid-glycolic acid copolymer and a penetration enhancer in a mass ratio of 1: (0.1-0.3). The skin-care gel provided by the invention has remarkable whitening, moisturizing and skin brightening effects.

The invention relates to the technical field of cosmetics, and particularly discloses emulsion type foaming cleaning scrub cream with a skin care effect and a preparation method of the emulsion type foaming cleaning scrub cream. The scrub cream is prepared from the following raw material components in percentage by mass: 8 to 15 percent of glycerol, 0.2 to 0.9 percent of sodium hydroxide, 0.3 to 0.8 percent of citric acid, 0.03 to 0.2 percent of EDTA (Ethylene Diamine Tetraacetic Acid) disodium, 0.5 to 1.5 percent of an auxiliary agent, 1.5 to 3.0 percent of a thickening agent A, 1.5 to 3.0 percent of a thickening agent B, 5.86 to 1457 percent of an emollient, 12 to 22 percent of a surface active foaming agent, 0.8 to 3.0 percent of an emulsifying agent, 2.5 to 13 percent of a cutin stripping agent, 0.7 to 1.3 percent of a preservative, 0.32 to 0.92 percent of a skin conditioning agent and the balance of water, the skin conditioner is a mixture prepared from sodium hyaluronate, tocopherol, papain, nicotinamide, carnosine, panthenol and phytosterol oleate.

The invention relates to application of a molecular marker related to pork flavor. The molecular marker is related to the content of alpha-nicotinamide adenine dinucleotide (NAD < + >) in longissimus dorsi of a pig. The invention discloses a method for identifying pork flavor and a breeding method for breeding pigs with better pork flavor. According to the method, the 131363728th nucleotide site of a pig to be detected on a chromosome 8 of a pig reference genome Sscrofa11.1 and GCF000003025.6 is detected, the genotype of the pig is judged, the pork flavor is identified, the content of alpha-nicotinamide adenine dinucleotide (NAD +) in longissimus dorsi muscle of a TT genotype at the site is higher than that of a pig individual of a CT genotype, and the content of the pig of the CT genotype is higher than that of a pig individual of a CC genotype. In actual breeding work, to-be-detected pigs with the 131363728 site genotype being TT can be selected as parents for breeding, and the method has important significance on breeding pigs with better pork flavor.

The invention relates to the technical field of skin care products, and discloses an anti-aging and whitening composition and a preparation method thereof.The anti-aging and whitening composition comprises anti-aging and whitening components, whitening synergistic components, moisturizing and repairing components and deionized water, and the anti-aging and whitening components comprise botryine, ergothioneine and Ectoine; wherein the mass ratio of the glassine to the ergothioneine to the ectoine is (1.2 to 2.0) to (0.4 to 0.7) to (1.0 to 1.5); the whitening synergistic component comprises one or more of nicotinamide, glabridin and tranexamic acid; the moisturizing and repairing component is prepared from one or more of sodium hyaluronate, ceramide NP, glycerol and 1, 3-butanediol. Experiments show that the mass ratio of the glassine to the ergothioneine to the ectoine is (1.2-2.0): (0.4-0.7): (1.0-1.5), so that the glassine, the ergothioneine and the ectoine show a remarkable synergistic effect (in some cases, the synergistic index CIlt is 0.85), and the anti-aging and whitening functions of the composition containing the glassine, the ergothioneine and the ectoine are improved.

The invention discloses a repairing mask containing Chinese rose hydrolat, which is prepared from the following components: the Chinese rose hydrolat, pure water, 1, 3-butanediol, allantoin, carbomer, sodium hyaluronate, xanthan gum, arginine, nicotinamide, 1, 2-pentanediol, 1, 2-hexanediol, octylene glycol, menthol, a carbohydrate gum composition, squalane nanoemulsion and a wrapping freshener. The invention discloses a production process of a repairing mask containing Chinese rose hydrolat. The repairing mask containing the Chinese rose hydrolat has the advantages of moisturizing, soothing and calming, nourishing skin, whitening and fading spots.

The invention belongs to the technical field of functional food and health food, and particularly relates to a composition with skin whitening and brightening effects and a preparation method thereof. The composition comprises rice peptide, orange osmanthus pollen, white tomato powder, tremella polysaccharide, pomegranate concentrated powder and nicotinamide. Experimental results show that the composition reduces synthesis of melanin by inhibiting the activity of tyrosinase and achieves the effects of whitening and brightening the skin.

The invention discloses an intelligent partitioned oil control composition specially researched for oil sensitive skin and application of the intelligent partitioned oil control composition, and belongs to the technical field of cosmetics. The oil control composition provided by the invention is prepared from the following components in parts by mass: 0.5 to 10 parts of a phellinus linteus source active ingredient, 0.5 to 10 parts of nicotinamide, 0.01 to 5 parts of panthenol, 0.1 to 10 parts of acetylchitosamine and 0.01 to 5 parts of glycosylglycerol, the active component of the medicinal phellinus igniarius source comprises at least one of a medicinal phellinus igniarius extract and a medicinal phellinus igniarius-lactobacillus plantarum bidirectional fermentation product. According to the invention, the components with proper mass parts are selected, and the components cooperate with one another to act together, so that oil control and moisture retention can be effectively realized, the skin barrier ability can be improved, and full-face intelligent partitioned oil control and moisture retention can be realized; the composition provided by the invention is mild and non-irritant, and is suitable for sensitive skin.

The invention relates to the technical field of cosmetics, in particular to preparation and application of a cyclodextrin-coated azelaic acid oil-control skin-care product. Comprising the following steps: S1, adding deionized water into a mixer, adding a cyclodextrin-azelaic acid inclusion compound, nicotinamide and allantoin, mixing to obtain a water phase, mixing glycerol, caprylic / capric triglyceride, polydimethylsiloxane, polysorbate-80 and tocopheryl acetate to obtain an oil phase, slowly adding the oil phase into the water phase, and shearing and emulsifying to obtain a nanoemulsion; and S2, cooling the nano-emulsion, adding carbomer while stirring, continuously cooling to room temperature, sequentially adding phenoxyethanol, ferulic acid, menthol and essence, adjusting the pH value of the emulsion with triethanolamine, homogenizing, defoaming, disinfecting and filling. By adjusting the proportion of the components in the formula, the skin feeling of the product can be further optimized, so that the product is more comfortable and not greasy, and a lasting oil control effect is provided.

The invention discloses a human small cell lung cancer brain metastasis organoid culture medium and a culture method. According to the culture medium, an advanced DMEM / F12 culture medium is used as a basic culture medium, and the following components are added: 2 to 5 [mu] M of GlutaMax, 8 to 10 [mu] M of HEPES, 1 * to 2 * of B27, 500 [mu] M of SB202190, 5 to 10 [mu] M of Y-27632, 0.5 [mu] M of A83-01, 0.5 to 2 [mu] M of N-Acetyl-L-cysteine, 5 mM of Nicotinamide, 25 to 100 ng / ml of recombinant human FGF7, 100 to 500 ng / ml of recombinant human FGF10, 100 to 200 ng / ml of Human Noggin, 50 to 500 ng / ml of Human R-Spondin-1, 50 to 100 ng / ml of According to the culture method, TryPLE Express is used for dissociation, the time is short, the activity of dissociated single cells is high, and the success rate of organoid culture can be increased to the maximum extent. Meanwhile, by combining with a culture medium with an optimized formula proportion, formation and growth of brain metastatic organs of the human small cell lung cancer can be promoted, and the construction success rate is increased.

The invention discloses a multifunctional face and chest care mask and a preparation method thereof, and belongs to the technical field of beauty and skin care. The mask adopts a split type design, comprises a face membrane and a chest membrane, and realizes six functions of moisturizing, cleaning pores, whitening and brightening, soothing and repairing, controlling oil and removing acnes, and resisting aging and tightening through innovative active component combination and a multi-layer structure. The face membrane comprises an adsorption layer, a slow release layer and a water locking layer; and the chest membrane adopts an elastic fiber substrate and a permeation-promoting microneedle structure, so that the absorption of active ingredients is enhanced. The active ingredients comprise a hyaluronic acid compound, nano-scale bentonite, nicotinamide, a centella asiatica extract, a salicylic acid derivative, collagen polypeptide and the like, and the comprehensive nursing effect is improved through the synergistic effect. The preparation method is combined with electrostatic spinning, micro-capsule wrapping and 3D printing technologies, and precise controlled release and personalized adaptation are achieved.